一种多功能集成化的单细胞蛋白质组学微流控芯片平台
By designing a three-layer microfluidic chip platform that integrates single-cell capture, cell lysis, and protein reduction functions, the problem of complex and time-consuming sample pretreatment in single-cell proteomics analysis is solved, enabling efficient, miniaturized, automated, and integrated online analysis.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- PEKING UNIV
- Filing Date
- 2024-03-05
- Publication Date
- 2026-07-17
AI Technical Summary
In single-cell proteomics analysis, sample pretreatment is complex, time-consuming, and results in significant losses, making it difficult to achieve efficient, miniaturized, automated, and high-throughput online analysis.
A three-layer microfluidic chip platform was designed, including a liquid channel layer, an isolation film layer, and a gas channel layer. The liquid flow is controlled by a pneumatic valve, and the platform integrates single-cell capture, cell lysis, protein reduction, and enzymatic digestion functions, and enables online sample pretreatment within the chip.
It improves the efficiency and accuracy of single-cell proteomics analysis, reduces sample loss, and enables efficient, miniaturized, automated, and integrated online analysis.
Smart Images
Figure CN120586946B_ABST