A test reaction vessel for evaluating the anaerobic toxicity of a homogenized material

Abstract

The application discloses an anaerobic toxicity evaluation test reaction dish for homogenized materials, which is used to solve the technical problem of how to shake the sample uniformly during the test process. The reaction dish comprises a water bath pot, a pot cover and a plurality of reaction bottles for containing the sample. The pot cover is provided with a driving mechanism and a loading mechanism for loading the reaction bottles. The driving mechanism comprises a power assembly, a vibration assembly and a rotating assembly. The rotating assembly comprises a middle shaft. The loading mechanism comprises a supporting plate arranged at the bottom end of the middle shaft and a plurality of hoops arranged outside the middle shaft. The vibration assembly comprises a pendulum. The device can simultaneously accommodate a plurality of reaction bottles. Therefore, the blank sample, the test sample and the parallel sample provided in the test can be loaded on the supporting plate. The reciprocating rotation and vibration of the supporting plate can simultaneously shake and oscillate all the reaction bottles to homogenize the samples in the bottles, so that the materials are fully homogenized, the consistency and reliability of the test are improved, and the test operation is more convenient.

Description

Technical Field

[0001] This invention relates to the field of anaerobic toxicity evaluation test equipment, and in particular to an anaerobic toxicity evaluation test reaction vessel for homogenizable materials. Background Technology

[0002] With the accelerating pace of industrialization and urbanization, the discharge of industrial wastewater containing organic pollutants is increasing daily, seriously threatening aquatic ecological security and human health. Anaerobic biological treatment technology, with its advantages of low energy consumption, low sludge production, and applicability to high-concentration organic wastewater, has been widely applied in wastewater treatment. In practical applications and research, accurately assessing the toxicological impact of organic matter or potentially toxic substances on anaerobic microbial systems is a crucial prerequisite for ensuring the stable operation of anaerobic treatment systems and optimizing process design. Therefore, conducting anaerobic toxicity evaluation experiments has become an important aspect of environmental monitoring and water treatment research.

[0003] Existing anaerobic toxicity assessment tests mainly employ batch reactors or anaerobic flasks. These devices have limitations in ensuring uniform material mixing, especially when conducting experiments containing particulate or highly viscous substances. Material sedimentation or stratification can easily occur, reducing the repeatability and reliability of experimental data. Furthermore, traditional reaction vessels typically lack effective material homogenization capabilities. Insufficient stirring during the reaction process often affects the microbial response to toxic substances, thus impacting the accuracy of toxicity assessment results.

[0004] Therefore, there is an urgent need for an anaerobic toxicity evaluation test vessel that can achieve full homogenization of materials and improve experimental consistency and reliability, so as to meet the current urgent needs of environmental monitoring and wastewater treatment research for efficient and accurate toxicity assessment. Summary of the Invention

[0005] In view of the above-mentioned prior art, the present invention provides a reaction vessel for homogenizing materials in the process of anaerobic toxicity evaluation test, and the main technical problem to be solved is how to facilitate the operation of shaking the sample during the test.

[0006] To achieve the above objectives, the technical solution of this invention is implemented as follows:

[0007] An anaerobic toxicity evaluation test vessel for homogenizable materials includes a water bath, a lid, and several reaction flasks for holding samples. The lid is equipped with a drive mechanism and a loading mechanism for mounting the reaction flasks. The drive mechanism includes a power component, a vibration component, and a rotation component.

[0008] The rotating assembly includes a central shaft, the loading mechanism includes a support plate at the bottom of the central shaft and several clamps on the outside of the central shaft, the vibration assembly includes a pendulum, the power assembly is used to drive the pendulum to rotate continuously and drive the central shaft to rotate in both directions, the central shaft shakes the reaction bottle by rotating in both directions, the pendulum causes vibration by striking the central shaft to agitate the water in the reaction bottle and the water bath, and the bottom of the support plate is provided with several baffles for stirring the water in the water bath.

[0009] Furthermore, the power assembly includes a cover fixedly connected to the top of the pot lid. A first through hole is provided at one end of each side of the cover. The same reciprocating screw is rotatably connected to the cover at the two first through holes. A motor is fixedly connected to the top of the pot lid at one side of the cover. The output shaft of the motor is fixedly connected to one end of the reciprocating screw. A housing is fixedly connected to the top of the pot lid outside the motor.

[0010] Furthermore, the pot lid has a second through hole in the middle of the inner side of the cover. The central shaft is rotatably connected to the pot lid in the second through hole. The central shaft is connected to a gear by a key on the outside of the rod in the cover. The reciprocating screw is connected to a nut by a thread on the outside. A rack is fixedly connected to one side of the nut. The rack meshes with the gear.

[0011] Furthermore, a third through hole is provided on one side of the cover, and a rotating shaft is rotatably connected to the cover at the third through hole. The rotating shaft is arranged parallel to the reciprocating screw. Both the rotating shaft and the reciprocating screw are connected to pulleys by keys at their outer ends, and the same belt is fitted around the two pulleys.

[0012] Furthermore, a connecting shaft is rotatably connected to the inner wall of the top of the casing. The bottom end of the connecting shaft and the outside of the rod body inside the casing are both connected to bevel gears by keys. The two bevel gears mesh. A turntable is fixedly connected to the outside of the connecting shaft. The pendulum is fixedly connected to the outside of the turntable. The material of the pendulum is rubber.

[0013] Furthermore, several collars are fixedly connected at equal intervals on the outer top of the pallet. The number of collars is the same as the number of clamps and they correspond one to one above the other. The clamp includes a housing and a limiting plate. A support rod is fixedly connected between the housing and the central shaft. A sliding groove is opened inside the housing, and the limiting plate is slidably connected to the housing at the sliding groove.

[0014] Furthermore, both the housing and the limiting piece are semi-circular rings. A lever is fixedly connected to the outer side of the limiting piece at one end outside the slide groove. Fixed posts are fixedly connected to the top and bottom of the limiting piece at the outer end outside the slide groove. A connecting piece of latex material is fixedly connected between the two fixed posts and the housing.

[0015] Furthermore, the limiting plate has lock holes at the top and bottom of the outer end of the slide groove. A lock plate is rotatably connected to the side of the housing away from the slide groove. The top and bottom of the inner side of the lock plate are fixedly connected with lock tongues that are compatible with the lock holes. The lock tongues are dog teeth shaped.

[0016] Furthermore, the reaction flask is equipped with a stopper at the mouth, and a fourth through hole is opened in the middle of the stopper. A latex tube is installed at the through hole of the stopper. Several through slots are opened on the pot lid. One end of the latex tube extends to the outside of the pot lid at the through slot. A column is fixedly connected to the four corners of the bottom outer side of the pot lid. The length of the column is equal to the depth of the water bath.

[0017] Furthermore, rubber gaskets are fixedly connected to the inner sides of both the housing and the limiting plate.

[0018] The beneficial effects of this invention are as follows:

[0019] 1. This device can accommodate multiple reaction flasks simultaneously, so blank samples, test samples, and parallel samples can all be installed on the tray. By using the reciprocating rotation and vibration of the tray, all reaction flasks can be shaken synchronously to homogenize the samples in the flasks, ensuring that the materials are fully homogenized. This improves the consistency and reliability of the experiment and makes the experimental operation more convenient. In addition, the reciprocating rotation and vibration of the tray can also stir and agitate the water in the water bath, making the contact between the water and the reaction flasks more thorough. This ensures the heating effect of the water bath during the experiment, thereby ensuring the full reaction of the test samples and promoting the discharge of gas, so as to accurately record the cumulative gas production at different times.

[0020] 2. By setting up a clamp and extending the limiting plate to the outside of the casing, the mouth of the reaction flask and the stopper can be simultaneously wrapped and fixed to ensure the stability of the reaction flask installation. When the limiting plate extends, it will also pull the two connecting plates to wrap around the outside of the reaction flask and the stopper respectively, thus ensuring the connection between the two during shaking, so as to maintain the airtightness of the reaction flask and ensure that the test process is stable and smooth.

[0021] 3. By setting rubber gaskets on the inside of both the casing and the limiting plate, the gaskets not only increase the friction between the reaction flask and the two, but also provide a protective effect for the reaction flask, thereby further ensuring the stability of the reaction flask installation. Attached Figure Description

[0022] Figure 1 This is a perspective view of a reaction vessel for an anaerobic toxicity evaluation test of a homogenizable material according to Example 1 of this application;

[0023] Figure 2 This is a cross-sectional view of the casing of a test vessel for anaerobic toxicity evaluation of a homogenizable material, as described in Example 1 of this application.

[0024] Figure 3 This is a bottom view of the tray of a test vessel for anaerobic toxicity evaluation of a homogenizable material, as described in Example 1 of this application.

[0025] Figure 4 This is a perspective view of the clamp of a reaction vessel for evaluating the anaerobic toxicity of a homogenizable material, as described in Example 1 of this application.

[0026] Figure 5 This is a perspective view of the clamp of a reaction vessel for evaluating the anaerobic toxicity of a homogenizable material, as described in Example 2 of this application.

[0027] Explanation of icon numbers:

[0028] 1. Water bath; 2. Column; 3. Boiler lid; 301. Through groove; 4. Housing; 5. Cover; 6. Belt; 7. Pulley; 8. Central shaft; 9. Support rod; 10. Clamp; 1001. Sleeve; 1002. Limiting plate; 11. Reaction bottle; 12. Collar; 13. Support plate; 1301. Paddle; 14. Reciprocating screw; 15. Rack; 16. Nut; 17. Rotating shaft; 18. Bevel gear; 19. Pendulum; 20. Connecting shaft; 21. Turntable; 22. Gear; 23. Connecting plate; 24. Paddle; 25. Fixing post; 26. Lock hole; 27. Locking plate; 28. Lock tongue; 29. ​​Washer. Detailed Implementation

[0029] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the invention.

[0030] Example 1

[0031] See attached document Figure 1-4 This application provides a test vessel for anaerobic toxicity evaluation of homogenizable materials, including a water bath 1, a lid 3, and several reaction bottles 11 for holding samples. The reaction bottles 11 are 250mL ground glass conical flasks. The lid 3 is provided with a driving mechanism and a loading mechanism for mounting the reaction bottles 11. The driving mechanism includes a power component, a vibration component, and a rotation component.

[0032] When conducting anaerobic toxicity evaluation tests, multiple concentration series of test solutions can be prepared using 2-butenal wastewater of different concentrations, 10 mL of sodium acetate solution with an LCD of 60 g / L, and 150 mL of nutrients. Then, 40 mL of anaerobic granular sludge and 210 mL of the above-mentioned test solutions of different concentration series are inoculated into different reaction flasks 11, and the pH value is adjusted to 6.8-7.2 with 2 mol / L NaOH solution. In this process, two parallel samples are prepared for each different concentration series of wastewater, and the series without wastewater is used as a blank sample.

[0033] Preferably, a number of collars 12 are fixedly connected at equal intervals on the outer side of the top of the pallet 13. The number of collars 12 is the same as the number of clamps 10 and they correspond one by one vertically. The clamp 10 includes a housing 1001 and a limiting piece 1002. A support rod 9 is fixedly connected between the housing 1001 and the central shaft 8. A sliding groove is opened inside the housing 1001, and the limiting piece 1002 is slidably connected to the housing 1001 at the sliding groove.

[0034] Preferably, the reaction flask 11 is provided with a stopper at the mouth of the flask. The stopper is a rubber stopper with a fourth through hole in the middle. The stopper is provided with a latex tube at the through hole. The other end of the latex tube is connected to a 50mL Schwarz fermentation tube. The lid 3 is provided with several through grooves 301. One end of the latex tube extends to the outside of the lid 3 at the through groove 301. The bottom of the lid 3 is fixedly connected to four corners of the bottom. The length of the column 2 is equal to the depth of the water bath 1. The column 2 can support the lid 3 so that the tray 13 will not fall to the bottom in the water bath 1. Therefore, the rotation of the lever 1301 provides space to move the water.

[0035] The reaction flask 11 can be sealed by using a stopper with a latex tube inserted inside. Then, different reaction flasks 11 are placed in several collars 12 on the support plate 13, with the mouth of the reaction flask 11 inside the casing 1001.

[0036] Preferably, the limiting piece 1002 has locking holes 26 at the top and bottom of the outer end of the slide groove. The side of the housing 1001 away from the slide groove is rotatably connected to a locking piece 27. The top and bottom of the inner side of the locking piece 27 are fixedly connected to a locking tongue 28 that matches the locking hole 26. The locking tongue 28 is dog-tooth shaped to prevent the locking tongue 28 from falling out of the locking hole 26 and to ensure a stable connection to the limiting piece 1002.

[0037] Afterwards, the limiting piece 1002 can be pulled out by moving the lever 24, and after its end contacts the other side of the housing 1001, the locking piece 27 is fastened to the outside of the limiting piece 1002. At this time, the locking tongue 28 can be inserted into the locking hole 26, thus fixing the limiting piece 1002. Therefore, the limiting piece 1002 can be fixed around the mouth of the reaction bottle 11.

[0038] Preferably, both the housing 1001 and the limiting piece 1002 are semi-circular rings. A paddle 24 is fixedly connected to the outer side of the limiting piece 1002 at one end outside the slide groove. Fixing posts 25 are fixedly connected to the top and bottom of the limiting piece 1002 at one end outside the slide groove. A connecting piece 23 of latex material is fixedly connected between the two fixing posts 25 and the housing 1001.

[0039] Furthermore, during the movement of the limiting piece 1002, the limiting piece 1002 can also stretch the two connecting pieces 23 to wrap around the outside of the reaction bottle 11 and the stopper by moving the two fixed posts 25. Since the connecting pieces 23 made of latex material tend to retract after being stretched, the elasticity of the two connecting pieces 23 is used to tightly clamp the reaction bottle 11 and the stopper to ensure a tight connection between the two and the airtightness of the reaction bottle 11.

[0040] Preferably, the power assembly includes a cover 5 fixedly connected to the top of the pot lid 3. A first through hole is provided at one end of each side of the cover 5. The cover 5 is rotatably connected to the same reciprocating screw 14 at the two first through holes. A motor is fixedly connected to one side of the cover 5 at the top of the pot lid 3. The output shaft of the motor is fixedly connected to one end of the reciprocating screw 14. A housing 4 is fixedly connected to the top of the pot lid 3 outside the motor. The housing 4 is also provided with a power supply and a controller electrically connected to the motor. The controller can control the oscillation frequency and shaking speed by adjusting the speed of the motor.

[0041] Preferably, the pot lid 3 has a second through hole in the middle of the inner side of the cover 5. The central shaft 8 is rotatably connected to the pot lid 3 at the second through hole. The central shaft 8 is connected to the gear 22 by a key on the outside of the rod in the cover 5. The reciprocating screw 14 is connected to the nut 16 by an external thread. A rack 15 is fixedly connected to one side of the nut 16. The rack 15 meshes with the gear 22.

[0042] After all the reaction flasks 11 are installed, the reaction flasks 11 and the tray 13 can be placed into the water bath 1 by moving the lid 3. Then, the temperature of the water bath 1 is adjusted to maintain at 36±1℃. Then, the motor in the housing 4 is started. The motor drives the reciprocating screw 14 to rotate, which causes the screw nut 16 to move back and forth in the horizontal direction. The screw nut 16 drives the rack 15 to move, which drives the gear 22 to rotate continuously in both directions. The gear 22 drives the tray 13 to rotate through the central shaft 8, so the reaction flasks 11 can be shaken.

[0043] Preferably, the rotating assembly includes a central shaft 8, the loading mechanism includes a support plate 13 disposed at the bottom of the central shaft 8 and several clamps 10 disposed on the outside of the central shaft 8, the vibration assembly includes a pendulum 19, the power assembly is used to drive the pendulum 19 to rotate continuously and drive the central shaft 8 to rotate forward and backward, the central shaft 8 shakes the reaction bottle 11 by rotating forward and backward, the pendulum 19 causes vibration by striking the central shaft 8 to agitate the water in the reaction bottle 11 and the water bath 1, and the bottom of the support plate 13 is provided with several baffles 1301 for stirring the water in the water bath 1.

[0044] Preferably, a third through hole is provided on one side of the cover 5, and a rotating shaft 17 is rotatably connected to the cover 5 at the third through hole. The rotating shaft 17 is arranged parallel to the reciprocating screw 14. Both the rotating shaft 17 and the reciprocating screw 14 are connected to pulleys 7 by keys at one end outside the cover 5. The same belt 6 is sleeved on the outside of the two pulleys 7.

[0045] Meanwhile, the rotation of the reciprocating screw 14 can also drive the rotating shaft 17 to rotate via the belt 6 and two pulleys 7. The rotating shaft 17 drives the connecting shaft 20 to rotate via two bevel gears 18. The connecting shaft 20 drives the pendulum 19 to rotate via the turntable 21, which can continuously strike the shaft 8, thereby causing the tray 13 to vibrate. Therefore, the tray 13 can vibrate the sample in the reaction bottle 11, thus cooperating with the shaking action to homogenize the sample inside, making the reaction more complete and more conducive to accurately recording the cumulative gas production at different times.

[0046] Preferably, a connecting shaft 20 is rotatably connected to the inner wall of the top of the cover 5. The bottom end of the connecting shaft 20 and the outside of the rod body of the rotating shaft 17 in the cover 5 are both connected by a key to bevel gears 18. The two bevel gears 18 mesh. A turntable 21 is fixedly connected to the outside of the connecting shaft 20. A pendulum 19 is fixedly connected to the outside of the turntable 21. The material of the pendulum 19 is rubber.

[0047] In addition, the vibration of the tray 13 can also agitate the water in the water bath 1, and its reciprocating rotation can also drive the lever 1301 to move the water, thus causing the water to flow and vibrate, ensuring sufficient contact between the water and the reaction bottle 11, thereby further ensuring the fullness of the reaction; the gas produced by the reaction will be discharged from the latex tube on the bottle stopper, so it is only necessary to connect one end of the latex tube to the fermentation tube to facilitate the collection and measurement of gas.

[0048] Example 2

[0049] See attached document Figure 5 This application provides an anaerobic toxicity evaluation test reaction vessel for homogenizable materials. Compared with Example 1, in order to further ensure the stability of the reaction bottle installation, rubber gaskets 29 are fixedly connected to the inner sides of the casing 1001 and the limiting piece 1002.

[0050] When the reaction flask 11 is fixed in the clamp 10, the gasket 29 on the inner side of the sleeve 1001 and the limiting piece 1002 can not only increase the friction between the two and the reaction flask 11, but also play a protective role for the reaction flask 11, thus further ensuring the stability of the reaction flask installation.

[0051] The above are merely specific embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention should be included within the scope of protection of the present invention. The scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A reaction vessel for evaluating the anaerobic toxicity of homogenizable materials, comprising a water bath (1), a lid (3), and several reaction flasks (11) for holding samples, characterized in that, The lid (3) is provided with a drive mechanism and a loading mechanism for mounting the reaction flask (11). The drive mechanism includes a power component, a vibration component and a rotation component. The rotating assembly includes a central shaft (8), the loading mechanism includes a support plate (13) at the bottom of the central shaft (8) and several clamps (10) on the outside of the central shaft (8), the vibration assembly includes a pendulum (19), the power assembly is used to drive the pendulum (19) to rotate continuously and drive the central shaft (8) to rotate in both directions, the central shaft (8) shakes the reaction bottle (11) by rotating in both directions, the pendulum (19) causes vibration by striking the central shaft (8) to agitate the water in the reaction bottle (11) and the water bath (1), and the bottom of the support plate (13) is provided with several baffles (1301) for stirring the water in the water bath (1). A number of collars (12) are fixedly connected at equal intervals on the outer side of the top of the pallet (13). The number of collars (12) is the same as the number of clamps (10) and they correspond one by one. The clamp (10) includes a housing (1001) and a limiting piece (1002). A support rod (9) is fixedly connected between the housing (1001) and the central shaft (8). A sliding groove is opened inside the housing (1001). The limiting piece (1002) is slidably connected to the housing (1001) at the sliding groove. Both the housing (1001) and the limiting piece (1002) are semi-circular rings. A paddle (24) is fixedly connected to the outer side of the limiting piece (1002) at one end outside the slide groove. Fixed posts (25) are fixedly connected to the top and bottom of the limiting piece (1002) at one end outside the slide groove. A connecting piece (23) of latex material is fixedly connected between the two fixed posts (25) and the housing (1001).

2. The anaerobic toxicity evaluation test vessel for homogenizable materials according to claim 1, characterized in that, The power assembly includes a cover (5) fixedly connected to the top of the pot lid (3). A first through hole is provided at one end of each side of the cover (5). The cover (5) is rotatably connected to the same reciprocating screw (14) at the two first through holes. A motor is fixedly connected to the top of the pot lid (3) at one side of the cover (5). The output shaft of the motor is fixedly connected to one end of the reciprocating screw (14). A housing (4) is fixedly connected to the top of the pot lid (3) outside the motor.

3. The anaerobic toxicity evaluation test vessel for homogenizable materials according to claim 2, characterized in that, The pot lid (3) has a second through hole in the middle of the inner side of the cover (5). The central shaft (8) is rotatably connected to the pot lid (3) at the second through hole. The central shaft (8) is connected to a gear (22) by a key on the outside of the rod body in the cover (5). The reciprocating screw (14) is connected to a nut (16) by a thread on the outside. A rack (15) is fixedly connected to one side of the nut (16). The rack (15) meshes with the gear (22).

4. The anaerobic toxicity evaluation test vessel for homogenizable materials according to claim 2, characterized in that, A third through hole is provided on one side of the cover (5). A rotating shaft (17) is rotatably connected to the cover (5) at the third through hole. The rotating shaft (17) is arranged parallel to the reciprocating screw (14). Both the rotating shaft (17) and the reciprocating screw (14) are connected to pulleys (7) by a key at one end outside the cover (5). The two pulleys (7) are fitted with the same belt (6).

5. The anaerobic toxicity evaluation test vessel for homogenizable materials according to claim 4, characterized in that, A connecting shaft (20) is rotatably connected to the inner wall of the top of the cover (5). The bottom end of the connecting shaft (20) and the outside of the rotating shaft (17) in the cover (5) are connected to bevel gears (18) by keys. The two bevel gears (18) mesh. A turntable (21) is fixedly connected to the outside of the connecting shaft (20). The pendulum (19) is fixedly connected to the outside of the turntable (21). The material of the pendulum (19) is rubber.

6. The anaerobic toxicity evaluation test vessel for homogenizable materials according to claim 1, characterized in that, The limiting piece (1002) has a lock hole (26) at the top and bottom of the outer end of the slide groove. The sleeve (1001) is rotatably connected to the side away from the slide groove. The top and bottom of the inner side of the lock piece (27) are fixedly connected to a lock tongue (28) that matches the lock hole (26). The lock tongue (28) is dog-tooth shaped.

7. The anaerobic toxicity evaluation test vessel for homogenizable materials according to claim 1, characterized in that, The reaction flask (11) is provided with a stopper at the mouth of the flask, and a fourth through hole is provided in the middle of the stopper. A latex tube is provided at the through hole of the stopper. Several through grooves (301) are provided on the pot lid (3). One end of the latex tube extends to the outside of the pot lid (3) at the through groove (301). A column (2) is fixedly connected to the four corners of the bottom of the pot lid (3). The length of the column (2) is equal to the depth of the water bath (1).

8. The anaerobic toxicity evaluation test vessel for homogenizable materials according to claim 1, characterized in that, The inner sides of the housing (1001) and the limiting piece (1002) are both fixedly connected with rubber gaskets (29).

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