一种心肌细胞内ATP水平动态监测方法及重组心肌细胞和应用
By constructing a recombinant cardiomyocyte line with iATPSnFR1, and utilizing the iATPSnFR1 sensor to specifically bind ATP within cardiomyocytes to generate fluorescence signals, the problem of traditional methods being unable to monitor ATP levels in real time was solved. This enabled non-invasive, real-time dynamic monitoring of ATP within cardiomyocytes, providing a more precise research tool.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SOUTHWEST MEDICAL UNIV
- Filing Date
- 2025-06-12
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies cannot achieve real-time, dynamic monitoring of ATP levels in cardiomyocytes. Traditional methods are highly invasive and prone to errors, failing to meet the research needs for dynamic changes in cardiomyocyte energy metabolism, especially in terms of real-time monitoring under pathophysiological conditions.
A cardiomyocyte cell line stably expressing iATPSnFR1 was constructed. The iATPSnFR1 gene was cloned into a plasmid and transfected into cardiomyocytes. The iATPSnFR1 sensor was used to specifically bind to ATP in the cells to generate a fluorescent signal, enabling non-invasive, real-time, and dynamic monitoring of ATP levels.
It enables real-time, dynamic monitoring of ATP levels in cardiomyocytes, is non-invasive and has high spatial resolution, providing a more accurate and reliable research tool suitable for studying cardiac ATP metabolism.
Smart Images
Figure CN120591343B_ABST