The invention relates to a method for synthesizing Cap2 structure 5apos; combinations and methods of capped RNA
By using a combination of tetranucleotide cap analogs and T7 RNA polymerase promoter sequences, the problems of low Cap2 capping rate and high cost were solved, efficient and economical Cap2 mRNA synthesis was achieved, and the safety and efficacy of mRNA were improved.
Patent Information
- Application Number
- CN202411750439.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-29
- Publication Date
- 2025-09-09
- Estimated Expiration
- 2044-11-29
AI Technical Summary
The existing technology lacks a low-cost and efficient Cap2 capping method. The Cap2 capping rate is low, the preparation is complex and the cost is high, which limits the advancement of Cap2 mRNA in basic research and application development.
A combination of a tetranucleotide cap analogue and a T7 RNA polymerase promoter sequence is used to form an efficient Cap2 structure 5'-capped RNA by in vitro transcription. The tetranucleotide cap analogue is used to form a transcription initiation combination with a DNA template, and the transcription reaction is carried out in the presence of T7 RNA polymerase.
The efficient synthesis of Cap2-capped RNA was achieved, the capping rate was increased, the production cost was reduced, and the safety and effectiveness of mRNA were improved.