Temperature and humidity controlled reaction chamber for inkjet printing of enzymatic DNA synthesis

Abstract

This invention discloses a temperature and humidity controlled reaction chamber for inkjet-printed enzymatic DNA synthesis. It integrates a chamber air inlet connection, a reaction area, a sliding top cover, and a chip fixing cover. The chamber air inlet connection uses multiple rows of parallel or intersecting air inlets to ensure uniform gas flow at a specific temperature and humidity into the reaction area. The DNA synthesis chip is placed in the reaction area, and the chip fixing cover ensures stable clamping of the DNA synthesis chip. The sliding top cover works in conjunction with the inkjet printhead. When the inkjet printhead dispenses the synthesis reagent onto the surface of the DNA synthesis chip, the top cover leaves the reaction area. After reagent dispensing is complete and the DNA enzymatic synthesis reaction begins, the top cover re-covers the reaction area, maintaining a relatively stable temperature, humidity, and gas environment in the synthesis reaction area. The reaction chamber can effectively control the surface temperature and humidity of the DNA synthesis chip, meeting the reaction conditions for enzymatic DNA synthesis, and simultaneously controlling the temperature and humidity environment of multiple chips.

Description

Technical Field

[0001] This invention relates to the field of inkjet-printed DNA synthesis, and in particular to a temperature and humidity controlled reaction chamber for inkjet-printed enzymatic DNA synthesis. Background Technology

[0002] DNA synthesis technology is a bottleneck technology in my country's development of synthetic biology. Currently, the methods for synthesizing DNA on the market are still mainly based on the first-generation chemical synthesis method proposed in the last century. However, the waste liquid generated by chemical DNA synthesis is toxic and produced in large quantities, requiring high standards for laboratory safety and waste liquid treatment, and it can easily have a certain impact on the environment. At present, the second-generation enzymatic DNA synthesis mediated by template-independent terminal deoxynucleotidyl transferases (TdT enzymes) is developing rapidly. Compared with the first-generation chemical synthesis technology, the synthesis reagents of the second-generation enzymatic DNA synthesis are aqueous solutions, which have the advantages of high synthesis efficiency, mild reaction conditions, and less environmental pollution. Currently, enzymatic DNA synthesis usually involves completely immersing the synthesis carrier, such as magnetic beads, in the liquid phase of the synthesis reagent, and the synthesis temperature needs to be controlled to ensure that the enzyme reaction rate reaches the optimal level.

[0003] Inkjet printing-based enzymatic DNA synthesis enables automation, further reducing enzyme and monomer consumption and achieving high-throughput DNA synthesis. This significantly promotes the development of enzymatic DNA synthesis; however, the technology is still in its early stages both domestically and internationally. When dispensing synthesis reagents using inkjet printing, the tiny droplets are prone to evaporation, necessitating strict humidity control of the DNA synthesis environment to minimize droplet evaporation, maintain reagent concentration, and ensure successful enzymatic DNA synthesis. Simultaneously, temperature control is crucial to ensure optimal enzyme activity and improve reaction efficiency. While temperature and humidity control can be achieved using a glove box associated with chemical DNA synthesis inkjet printers, the large size of the glove box makes it difficult to meet the temperature and humidity requirements, and excessive humidity can damage the inkjet printing unit. Currently, there is a lack of miniaturized and automated devices capable of efficiently and stably controlling the temperature and humidity of DNA synthesis chips for inkjet printing-based enzymatic DNA synthesis. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to provide a temperature and humidity controlled reaction chamber for inkjet printing of enzymatic DNA synthesis.

[0005] To solve the above-mentioned technical problems, the technical solution proposed by the present invention is as follows: A temperature and humidity controlled reaction chamber for enzymatic DNA synthesis in inkjet printing includes a chamber air inlet connection 101, a first reaction region 102, a second reaction region 103, a slidable top cover 104, and a chip fixing cover 105. The chamber air inlet connection 101 is fixed between the first reaction region 102 and the second reaction region 103. The slidable top cover 104 is placed above the chamber air inlet connection 101, the first reaction region 102, and the second reaction region 103 and is slidably connected. The chip fixing cover 105 is disposed within the first reaction region 102 and the second reaction region 103.

[0006] The aforementioned temperature and humidity control reaction chamber for inkjet-printed enzymatic DNA synthesis, combined with a humidity generator and other equipment providing gases with specific humidity and temperature, can stabilize the temperature and humidity within the reaction chamber, providing the environmental conditions necessary for inkjet-printed enzymatic DNA synthesis and effectively promoting the further development of enzymatic DNA synthesis and its automation.

[0007] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the chamber air inlet connection 101 includes a two-position three-way solenoid valve 201, a gas conduit 202, an air inlet chamber 203, and an air inlet connecting plate 204. A partition 205 is provided in the middle of the air inlet chamber 203, dividing it into a left chamber and a right chamber. The air inlet chamber 203 is located between the first reaction region 102 and the second reaction region 103. The left chamber is connected to the first reaction region 102 via the air inlet connecting plate 204 on one side. The reaction zone 102 is fixedly connected, and the right chamber is fixedly connected to the second reaction zone 103 through the air inlet connecting plate 204 on one side. The air inlet connecting plate 204 is symmetrically arranged on both sides of the air inlet chamber 203. The two-position three-way solenoid valve 201 includes an air inlet channel and two air outlet channels. The three channels are respectively connected to the humidity generator 5 and the left and right chambers of the air inlet chamber 203 through the gas conduit 202. The two-position three-way solenoid valve controls the gas from the humidity generator 5 to only connect to one side of the air inlet chamber 203 at the same time.

[0008] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing of enzymatic DNA synthesis, the main structure of the air inlet chamber 203 is a rectangular chamber structure. Two circular holes are opened on the front wall of the chamber for connecting gas conduits 202. A partition 205 is set in the center of the chamber to buffer the gas, ensuring that the gas in the two chambers is not interconnected, while being connected to their respective synthesis reaction areas. The air inlet connecting plate is a rectangular plate with a rectangular through hole in the middle. The size of the through hole matches the external size of the air inlet chamber. The air inlet connecting plate can be sealed and fixed to the outer periphery of both sides of the air inlet chamber by adhesive bonding, completing the assembly of the main body of the chamber air inlet connecting part. The air inlet connecting plate 204 is sealed and connected to the first reaction area 102 / second reaction area 103 by clamping gaskets to ensure airtightness.

[0009] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the air inlet connecting plate 204 is a separate cuboid structure installed on both sides of the air inlet chamber 203. The rectangular through hole in the middle of the air inlet connecting plate 204 is the same size as the air inlet chamber 203, which facilitates the air inlet connecting plate 204 to be accurately fitted into both sides of the air inlet chamber 203 and sealed and fixed. The air inlet connecting plate 204 is tightly assembled with the first reaction area 102 / second reaction area 103 through clamping gaskets.

[0010] Preferably, in the temperature and humidity controlled reaction chamber for inkjet-printed enzymatic DNA synthesis, the first reaction area 102 / second reaction area 103 has a scoop-shaped structure, including a perforated connecting wall 301, an upper cover supporting side wall 302, and a lower chamber wall composed of a chip placement platform 303 and a bottom slope 304. The perforated connecting wall 301 is located at one end of the bottom slope 304 of the lower chamber wall. The upper cover supporting side wall 302 is symmetrically arranged on both sides of the bottom slope 304 of the lower chamber wall and fixedly connected to both sides of the perforated connecting wall 301. The bottom slope 304 is adjacent to the upper cover supporting side wall 302, and each side is provided with a chip placement platform 303 for supporting and fixing the DNA synthesis chip. The thickness at the connection position between the bottom slope 304 and the perforated connecting wall 301 gradually decreases along the direction away from the perforated connecting wall 301, so that the bottom slope 304 forms a downward sloping angle with the horizontal plane.

[0011] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the pore array connecting wall 301 is located at one end of the scoop-shaped structure. The pore array connecting wall 301 has an array of air inlets arranged in a parallel or cross pattern in the middle. Each air inlet has the same diameter, and the center-to-center distance between adjacent air inlets in each horizontal and vertical row is equal to ensure that the airflow flows into the reaction area uniformly and stably.

[0012] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, a long strip slide rail is provided above the upper cover support sidewall 302. The slide rail has an isosceles triangle cross section and cooperates with the triangular recess of the slidable upper cover 104 to support the upper cover, ensuring smooth sliding of the upper cover between the first reaction area 102 and the second reaction area 103.

[0013] Preferably, in the temperature and humidity controlled reaction chamber for inkjet-printed enzymatic DNA synthesis, the lower wall of the chamber is composed of a chip placement platform 303 and a bottom slope 304. The width of the lower wall is equal to the length of the perforated connecting wall 301 minus the thickness of the side upper cover support walls 302. A chip placement platform 303 is provided on each lower wall adjacent to the side upper cover support walls to support and fix the DNA synthesis chip. A long protrusion is provided on each of the two chip placement platforms 303 adjacent to the side upper cover support walls to cooperate with the chip fixing cover 10. 5. The DNA synthesis chip is fixed and clamped, ensuring its stability during chamber movement, reagent spraying, and gas purging. Between the two chip placement platforms 303 is a bottom slope 304, which forms a downward angle with the horizontal plane. This ensures that the waste liquid generated by the DNA synthesis reaction can flow smoothly along the bottom slope 304 into the waste liquid tank set below the chamber, achieving waste liquid collection. The bottom slope 304 is coated with a hydrophobic coating to increase its hydrophobicity, improve waste liquid collection efficiency, and reduce the risk of waste liquid accumulation.

[0014] Preferably, in the temperature and humidity controlled reaction chamber for inkjet-printed enzymatic DNA synthesis described above, the chip fixing cover 105 includes two types: a rectangular cover 501 and a rotatable clamping cover 502. The rectangular cover 501 has a larger contact area with the DNA synthesis chip, enabling more stable clamping; the rotatable clamping cover 502 offers advantages such as convenient installation of the DNA synthesis chip without the need for repeated disassembly.

[0015] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the sliding cover structure 104 includes a cover body 401, an air inlet baffle 402, and a cover handle 403. The air inlet baffle 402 is fixed on both sides of the cover body 401, the cover handle 403 is located on the upper surface of the cover body 401, and the cover body 401 is provided with a cover exhaust port 404.

[0016] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the upper cover body 401 serves as the main structure of the sliding upper cover, spanning the two reaction areas and the chamber air inlet connection. When the upper cover completely covers one side of the reaction area for reaction, the gas flowing through the reaction area will be discharged through the upper cover exhaust port 404, preventing the accumulation of gas in the reaction area.

[0017] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the air inlet baffle 402 is located on both sides of the slidable top cover 104, so that the air inlet baffle 402 can completely cover the arrayed air inlets. The air inlet baffle 402 has concave grooves 405 on both sides. The entire slidable top cover 104 has four concave grooves, which are triangular in shape and have the same size as the triangular cross section of the long slide rail, forming a concave-convex fit to ensure that the slidable top cover 104 slides smoothly on the slide rail 305.

[0018] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the upper cover body 401 serves as the main structure of the sliding upper cover 104, spanning the chamber air intake connection 101 and the two reaction areas.

[0019] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the air inlet baffles 402 are located on both sides of the top cover. The area enclosed by the lower edge and the left and right edges of the baffles can completely cover the array of small air inlets. The air inlet baffles are also provided with concave grooves 405 on both sides. The entire slidable top cover 104 has four concave grooves, which are in the shape of concave triangles and have the same size as the triangular cross section of the long slide rail, forming a concave-convex fit to ensure that the slidable top cover 104 slides smoothly on the slide rail.

[0020] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the top cover handle 403 is located at two corners on the same side of the top cover body and is connected to the small lead screw stepper motor 2 by a connecting rod 3.

[0021] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, a small wireless temperature and humidity sensor is provided on the inner surface of the upper cover body 401, and the small wireless temperature and humidity sensor is signal-connected to the humidity generator 5.

[0022] The aforementioned temperature and humidity control reaction chamber for inkjet-printed enzymatic DNA synthesis includes a small wireless temperature and humidity sensor probe located inside the reaction area to detect the temperature and humidity of the gas inside the reaction chamber and facilitate timely feedback and adjustment. This sensor provides temperature and humidity measurement signals for feedback control of the gas inside the chamber and allows for real-time monitoring of the temperature and humidity of the gas.

[0023] Preferably, the temperature and humidity controlled reaction chamber for inkjet printing of enzymatic DNA synthesis is made of transparent acrylic material. In addition, other materials that can be used for manufacturing, such as resin, PEEK, and PLA plastic, can also be used.

[0024] Preferably, in the above-mentioned temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the chamber air inlet connection 101 and the first reaction area 102 / second reaction area 103 are completely sealed by clamping a sealing sheet in the middle and then screwing in a screw. Alternatively, any known gap sealing method, including adhesives, pressure, or tightening, can be used to achieve a complete seal.

[0025] Preferably, the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis has threaded holes on the chamber air inlet connection 101, the first reaction area 102, the second reaction area 103, and the chip fixing cover 105, and a sealing plate of corresponding size is provided on them. The reaction chamber 1 is assembled as a whole by clamping the sealing plate and tightening the screw.

[0026] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the sealing sheet is made of an elastic material, including but not limited to PDMS, silicone, polysilane materials, polyurethane, acrylate, polyesterimide, nitrile rubber, acrylic rubber, etc.

[0027] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the cross-section of the slide rails on the upper cover support sidewalls 302 of the first reaction area 102 and the second reaction area 103 is triangular. In addition, any shape that can form a convex-concave fit and be stable, such as a semi-circular arc, elliptical arc, rectangle, trapezoid, or serrated shape, can also be used.

[0028] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the arrayed air inlets on the pore array connecting wall 301 can have a variety of shapes, including but not limited to one of the following: circular, triangular, square, rectangular, pentagonal, hexagonal, octagonal, other regular polygons, or honeycomb.

[0029] Preferably, the temperature and humidity control reaction chambers for inkjet-printed enzymatic DNA synthesis described above are arranged in series and parallel, making the chambers scalable and capable of synthesizing more DNA synthesis chips simultaneously.

[0030] The aforementioned temperature and humidity controlled reaction chamber for inkjet-printed enzymatic DNA synthesis requires external equipment for operation, including: a small lead screw stepper motor 2, a connecting rod 3, an inkjet printer 4, a humidity generator 5, and a compressed gas cylinder 6. The linear movement of the small lead screw stepper motor 2 drives the connecting rod 3 to automatically move the sliding cover 104. The inkjet printer 4 precisely distributes the four nucleotide monomers (A, T, C, G) for enzymatic DNA synthesis, and provides deprotection and cleaning reagents, as well as gas drying functions. The gas generated by the compressed gas cylinder 6, after passing through the humidity generator 5, carries a certain temperature and humidity. After passing through a gas conduit 202 wrapped with a heating tape, the gas is stably input into the reaction chamber 1 by adjusting the cylinder pressure.

[0031] Technical effect The aforementioned temperature and humidity controlled reaction chamber for inkjet-printed enzymatic DNA synthesis comprises four parts: a chamber air inlet connection, a reaction area, a sliding top cover, and a chip fixing cover. It integrates functions such as stable airflow input, chip fixation, and stable temperature and humidity maintenance. The chamber air inlet connection, through multiple rows of parallel or intersecting air inlets, ensures uniform gas flow at a specific temperature and humidity into the reaction area. The reaction area holds the DNA synthesis chip, and the chip fixing cover ensures stable clamping of the chip. The sliding top cover works in conjunction with the inkjet printhead. When the inkjet printhead dispenses the synthesis reagents onto the surface of the DNA synthesis chip, the top cover leaves the reaction area. After reagent dispensing is complete and the DNA enzymatic synthesis reaction begins, the top cover re-covers the reaction area, maintaining a relatively stable temperature, humidity, and gas environment in the synthesis reaction area. This reaction chamber can effectively control the temperature and humidity on the surface of the DNA synthesis chip, meeting the reaction conditions for enzymatic DNA synthesis. Furthermore, the reaction chamber can be easily expanded by connecting the same device in series and parallel, and the temperature and humidity environment of multiple chips can be controlled simultaneously. It has the advantages of simple operation, automation, and scalability, and solves the problems of easy droplet evaporation, reaction temperature and humidity control, and waste liquid outflow in the inkjet printing enzymatic DNA synthesis process, ensuring the smooth progress of the enzymatic DNA synthesis process. Attached Figure Description

[0032] Figure 1 This is a diagram of the temperature and humidity control reaction chamber and its peripheral control system for inkjet-printed enzymatic DNA synthesis as described in this invention.

[0033] Figure 2A schematic diagram of the temperature and humidity control reaction chamber for inkjet-printed enzymatic DNA synthesis.

[0034] Figure 3 This is a structural schematic diagram of the air intake connection part of the cabin.

[0035] Figure 4 This is a structural schematic diagram of the reaction area in the compartment.

[0036] Figure 5 This is a schematic diagram of the sliding top cover.

[0037] Figure 6 A schematic diagram of the structure for fixing the chip cover.

[0038] Figure 7 This is a schematic diagram showing the assembly of the chamber reaction area, the DNA synthesis chip, and the fixing cover.

[0039] Figure 8 This is a schematic diagram of the air intake chamber and the air intake connecting plate in the cabin air intake connection section.

[0040] Figure 9 This is a schematic diagram of the reaction area in the compartment.

[0041] Figure 10 A schematic diagram of the complex formed by the reaction chamber, the DNA synthesis chip, and the fixation cover plate, as well as the DNA synthesis chip and the fixation cover plate.

[0042] Figure 11 This is a schematic diagram of a sliding top cover.

[0043] In the diagram: 1-Reaction chamber, 2-Small lead screw stepper motor, 3-Connecting rod, 4-Inkjet printer, 5-Humidity generator, 6-Compressed gas cylinder, 101-Chamber air inlet connection, 102-First reaction zone, 103-Second reaction zone, 104-Sliding top cover, 105-Chip fixing cover, 201-Two-position three-way solenoid valve, 202-Gas conduit, 203-Air inlet chamber, 204-Air inlet connecting plate, 205-Block, 301-Perforated array connection 302-Top cover support sidewall, 303-Chip placement platform, 304-Bottom slope, 401-Top cover body, 402-Air inlet baffle, 403-Top cover handle, 404-Top cover exhaust port, 405-Concave groove, 501-Rectangular cover, 502-Rotating clamping cover, A-Length of air inlet chamber, B-Width of air inlet chamber, C-Height of air inlet chamber, D-Length of air inlet connecting plate, E-Width of air inlet connecting plate, F-Length of top cover support sidewall, G- Width of the top cover supporting sidewall, H - Thickness of the connection point between the bottom slope and the aperture array connecting wall, I - Thickness of the aperture array connecting wall, K - Height of the slide rail, L - Width of the lower wall of the chamber, M - Width of the chip placement platform, N - Width of the boss, H1 - Height of the boss, H2 - Thickness of the DNA synthesis chip, H3 - Thickness of the fixing cover, O - Length of the DNA synthesis chip, W1 - Width of the DNA synthesis chip, P - Length of the fixing cover, W2 - Width of the fixing cover, T - Width of the gap between the two DNA synthesis chips. Dimensions: Q - Length of the sliding top cover; R - Length of the lower edge of the air intake baffle; S - Height of the left and right edges of the air intake baffle; Y - Thickness of the air intake baffle; a - Wall thickness of the air intake chamber; d - Diameter of the circular hole; e - Diameter of the arrayed air intake holes; f - Center distance between holes in the horizontal row; g - Center distance between holes in the vertical row; α - Number of air intake holes in the horizontal row; β - Number of air intake holes in the vertical row; p - Center distance between adjacent threaded holes of the boss; u - Length of the exhaust port of the top cover; v - Width of the exhaust port of the top cover; w - Distance from the exhaust port of the top cover to the boundary of the top cover. Detailed Implementation

[0044] The following detailed description, in conjunction with embodiments and accompanying drawings, illustrates the temperature and humidity control reaction chamber for inkjet-printed enzymatic DNA synthesis according to the present invention. In the embodiments, the present invention can employ various materials for chamber fabrication, and the DNA synthesis chip used is a glass slide modified with primer chains.

[0045] Example 1 like Figure 1-11As shown, the temperature and humidity controlled reaction chamber for inkjet-printed enzymatic DNA synthesis includes a chamber air inlet connection 101, a first reaction area 102, a second reaction area 103, a slidable top cover 104, and a chip fixing cover 105. The chamber air inlet connection 101 is fixed between the first reaction area 102 and the second reaction area 103. The slidable top cover 104 is placed above the chamber air inlet connection 101, the first reaction area 102, and the second reaction area 103 and is slidably connected. The chip fixing cover 105 is disposed within the first reaction area 102 and the second reaction area 103. The main functions of each part are as follows: the chamber air inlet... The gas connection part 101 can ensure that the airflow is evenly introduced into the first reaction area 102 and the second reaction area 103 respectively; the first reaction area 102 and the second reaction area 103 are the positions for placing the DNA synthesis chip and carrying out the enzymatic DNA synthesis reaction, and together with the chip fixing cover 105, they can ensure the stable clamping of the DNA synthesis chip; the sliding cover 104 can cooperate with the inkjet printing device 4. When the synthesis reagent is distributed to the surface of the DNA synthesis chip by the inkjet printing device 4, the cover leaves the reaction area. When the reagent is distributed and the DNA enzymatic synthesis reaction is carried out, the cover covers the reaction area again, so that the synthesis reaction area maintains a relatively stable temperature, humidity and gas environment.

[0046] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the chamber air inlet connection 101 includes a two-position three-way solenoid valve 201, a gas conduit 202, an air inlet chamber 203, and an air inlet connecting plate 204. A partition 205 is provided in the middle of the air inlet chamber 203, dividing it into a left chamber and a right chamber. The air inlet chamber 203 is located between the first reaction region 102 and the second reaction region 103. The left chamber is connected to the first reaction region 102 via the air inlet connecting plate 204 on one side. The reaction zone 102 is fixedly connected, and the right chamber is fixedly connected to the second reaction zone 103 through the air inlet connecting plate 204 on one side. The air inlet connecting plate 204 is symmetrically arranged on both sides of the air inlet chamber 203. The two-position three-way solenoid valve 201 includes an air inlet channel and two air outlet channels. The three channels are respectively connected to the humidity generator 5 and the left and right chambers of the air inlet chamber 203 through the gas conduit 202. The two-position three-way solenoid valve controls the gas from the humidity generator 5 to only connect to one side of the air inlet chamber 203 at the same time.

[0047] The main structure of the air intake chamber 203 is a rectangular chamber structure. The external length of the air intake chamber 203 is A, the width is B, and the height is C. The thickness of each wall surface of the chamber is a. Two circular holes with a diameter of d are opened on the front wall of the chamber for connecting the gas conduit 202. A partition 205 is set in the center of the chamber to buffer the gas, ensuring that the gas in the two chambers is not interconnected, while being connected to their respective synthesis reaction areas. The air intake connecting plate is a rectangular plate with a length of D, a width of E, and a thickness of b. A long... A rectangular through hole with a width of B and a height of C is provided. The size of the through hole matches the external dimensions of the air intake chamber. The air intake connecting plate can be sealed and fixed to the outer periphery of both sides of the air intake chamber by adhesive bonding, thus completing the assembly of the main body of the air intake connecting part of the chamber. The outer periphery of the air intake connecting plate is provided with symmetrical threaded holes on the top and bottom and left and right sides. By clamping a gasket between the air intake connecting plate 204 and the first reaction area 102 / second reaction area 103 and tightening it with screws, the airtight connection between the air intake connecting part of the chamber and the reaction area is achieved, ensuring airtightness. The intake connecting plate 204 is a separate cuboid structure installed on both sides of the intake chamber 203. The intake connecting plate 204 is a rectangular plate with a length of D, a width of E, and a thickness of b. The rectangular through hole in the middle is the same size as the intake chamber 203. Its cross-section is B in length and C in width, which allows the intake connecting plate 204 to be accurately fitted into both sides of the intake chamber 203. It can also be further fixed and sealed by adhesive sealing. The intake connecting plate 204 is also provided with threaded holes. It is assembled by clamping a gasket between the intake connecting plate 204 and the first reaction area 102 / second reaction area 103 and tightening it with screws.

[0048] The aforementioned temperature and humidity controlled reaction chamber for inkjet-printed enzymatic DNA synthesis has a sieve-shaped structure in the first reaction region 102 and the second reaction region 103. It includes a perforated connecting wall 301, an upper cover supporting sidewall 302, and a lower chamber wall composed of a chip placement platform 303 and a bottom slope 304. The perforated connecting walls 301 are located at one end of the bottom slope 304 of the lower chamber wall. The upper cover supporting sidewalls 302 are symmetrically arranged on both sides of the bottom slope 304 of the lower chamber wall and fixedly connected to both sides of the perforated connecting walls 301. The bottom slope 304 is adjacent to the upper cover supporting sidewall 302, and each side has a chip placement platform 303 for supporting and fixing the DNA synthesis chip. The thickness of the bottom slope 304 at the connection point with the perforated connecting wall 301 gradually decreases away from the perforated connecting wall 301, creating a downward-sloping angle between the bottom slope 304 and the horizontal plane. Specifically… The perforated connecting wall 301 is located at one end of the scoop-shaped structure, with a length of D, a width of E, and a thickness of I. The connecting wall has an array of air inlets arranged in a parallel or intersecting pattern in its middle section. Each air inlet has the same diameter, and the center-to-center distance between adjacent air inlets in each horizontal and vertical row is equal to ensure uniform and stable airflow into the reaction area. Each air inlet has a diameter of e, each horizontal row has α air inlets with a center-to-center distance of f, and each vertical row has β air inlets with a center-to-center distance of g. When the air inlet connecting plate 204 and the perforated connecting wall 301 are clamped together with a gasket and tightened with screws, the area covered by the air inlet array corresponds to the ventilation area inside the air inlet chamber 203, i.e., satisfying the following conditions:

[0049]

[0050] This ensures effective ventilation of the arrayed air inlets, allowing all gas exiting the air inlet chamber 203 to flow into the first reaction region 102 / second reaction region 103 without accumulation. The upper cover support sidewall 302 has a length of F, a width of G, and a height of E. A long, narrow slide rail with an isosceles triangle cross-section is provided above the sidewall, with a base length of G, a height of K, and a length of F. Its functions are twofold: first, to cooperate with the triangular recess of the sliding upper cover 104 to support the upper cover; and second, to ensure smooth sliding of the upper cover between the first reaction region 102 and the second reaction region 103. The lower wall of the chamber consists of a chip placement platform 303 and a bottom inclined surface 304. The lower wall has a length of F and a width of L. The width of the lower wall is equal to the length of the perforated array connecting wall 301 minus the thickness of the two upper cover support sidewalls 302.

[0051] On the lower wall of the chamber, adjacent to the upper cover support sidewall, are two chip placement platforms 303, each with a length of F and a width of M, for supporting and fixing the DNA synthesis chip. On each of the two chip placement platforms 303, adjacent to the upper cover support sidewall, are two protrusions with a length of F, a width of N, and a height of H1. Threaded holes with a center-to-center distance of p are provided on these protrusions to work with the chip fixing cover 105 to clamp and secure the DNA synthesis chip, ensuring stability during chamber movement, reagent spraying, and gas purging. Between the two chip placement platforms 303 is a space with a length of F and a width of M. The bottom inclined surface 304 has a thickness of H at the connection point with the aperture array connecting wall 301. The thickness of the bottom inclined surface 304 gradually decreases away from the aperture array connecting wall 301, creating a downward-sloping angle between the bottom inclined surface 304 and the horizontal plane. This ensures that the waste liquid generated by the DNA synthesis reaction can flow smoothly along the bottom inclined surface 304 into the waste liquid tank located below the chamber, achieving waste liquid collection. A hydrophobic coating can be applied to the bottom inclined surface 304 to increase its hydrophobicity, improve waste liquid collection efficiency, and reduce the risk of waste liquid accumulation. The structural dimensions of the lower wall of the chamber are determined based on the length O, width W1, and thickness H2 of the DNA synthesis chip. The number of DNA synthesis chips placed side-by-side in the cabin is X (X is greater than or equal to 1). To thoroughly remove the waste liquid generated by the DNA synthesis reaction from the chip surface, a gap of width T is set between the chips. Simultaneously, to avoid interference with the temperature and humidity of the synthesis chip surface by the exhaust vent 404 on the top cover, the DNA synthesis chips are only placed in the area between the aperture connecting wall 301 and the exhaust vent 404 on the top cover. Therefore, the following conditions are met:

[0052] The chip placement platform 303 is designed to support and immobilize the DNA synthesis chip, therefore its dimensions must meet the following requirements:

[0053]

[0054] In addition, the thickness H of the connection point between the inclined surface 304 at the bottom of the chamber and the pore array connecting wall 301 must ensure that, after the DNA synthesis chip is placed, it does not exceed the height of the lower edge of the arrayed air inlet from the bottom of the chamber, i.e.:

[0055] Otherwise, it will affect the temperature and humidity of the gas entering the synthesis reaction zone.

[0056] The aforementioned temperature and humidity controlled reaction chamber for inkjet-printed enzymatic DNA synthesis includes two types of chip fixing covers 105: a rectangular cover 501 and a rotatable clamping cover 502. The chip fixing cover 105 is a rectangular cover 501 with a length W2, width P, and thickness H3. One side of the cover has a threaded hole with a center-to-center distance p, which mates with a threaded hole on the chip placement platform 303. To ensure the cover can firmly press the DNA synthesis chip, its length is equal to the chip width, i.e.:

[0057] Its width is greater than the width of the boss, that is:

[0058] The advantage of this rectangular cover is that it has a large contact area with the DNA synthesis chip, enabling stable clamping. Furthermore, the length of the cover can be adjusted according to the number of chips to achieve single-chip coverage, or a longer cover can be used to cover multiple chips simultaneously.

[0059] The chip fixing cover 105 is a rotatable clamping cover 502, which consists of a flat plate structure and an arc-shaped structure. The flat plate structure is semi-circular to ensure that the cover will not hit the upper cover support side wall 302 during rotation. The rotatable clamping cover is provided with a single threaded hole for fixing it to the protrusion of the chip placement platform 303. This rotatable clamping cover has the advantage of facilitating the installation of DNA synthesis chips without having to disassemble them each time.

[0060] The aforementioned temperature and humidity controlled reaction chamber for inkjet-printed enzymatic DNA synthesis includes a sliding cover structure 104 comprising a cover body 401, an air inlet baffle 402, and a cover handle 403. The cover body 401, as the main structure of the sliding cover, spans the two reaction zones and the chamber air inlet connection. Its total length Q is equal to the total length of the air inlet chamber 203, the thickness of one side of the first reaction zone 102 / second reaction zone 103, and the thickness of the air inlet baffle 402, i.e., satisfying:

[0061] Simultaneously, exhaust ports 404 with length u and width v are respectively provided at both ends of the upper cover body 401 at a distance w from its boundary. When the upper cover completely covers one side of the reaction area for reaction, the gas flowing through the reaction area will be discharged through the exhaust ports 404, preventing gas accumulation in the reaction area. The air inlet baffles 402 are located on both sides of the slidable upper cover 104. The lower edge of the air inlet baffle has a length R, the left and right edges have a height S, and the thickness of the baffle is Y, satisfying:

[0062]

[0063] The purpose is to ensure that the air inlet baffle 402 completely covers the arrayed air inlets, protecting them from contamination by droplet splashes during the cleaning and drying of the DNA synthesis chip; simultaneously, it must prevent collisions with the chip fixing cover on the chip placement platform 303 during the sliding of the top cover. It is worth noting that the top cover exhaust port 404 is located above the area away from the arrayed air inlets within the reaction area. To avoid interference with the temperature and humidity of the synthesis chip surface from the top cover exhaust port 404, the DNA synthesis chip is not placed in this area. The air inlet baffle 402 has concave grooves 405 on both sides. The entire sliding top cover 104 has four concave grooves, forming a triangular shape. Their dimensions are the same as the triangular cross-section of the elongated slide rail, allowing for a convex-concave fit to ensure smooth sliding of the sliding top cover 104 on the slide rail 305. The main body 401 of the upper cover serves as the main structure of the sliding upper cover 104, spanning the air intake connection 101 of the compartment and two reaction zones (first reaction zone 102 and second reaction zone 103). Its total length Q is equal to the sum of the thickness of the air intake chamber 203, one side reaction zone (first reaction zone 102 or second reaction zone 103), and the thickness of the air intake baffle 402. At the same time, upper cover exhaust ports 404 with a length of u and a width of v are respectively provided at both ends of the upper cover main body 401 at a distance w from its boundary. When the upper cover completely covers one side reaction zone (first reaction zone 102 or second reaction zone 103) for reaction, the upper cover exhaust port 404 is located above the tail of the reaction zone. The gas flowing through the reaction zone will be discharged through the upper cover exhaust port 404, which will not cause the gas to accumulate in the reaction zone. The air inlet baffle 402 is located on both sides of the top cover. The area enclosed by the lower edge and the left and right edges of the baffle can completely cover the array of small air inlets. The lower edge of the baffle is R in length and the left and right edges are S in height. During the cleaning and drying of the DNA synthesis chip, the air inlets are protected from contamination caused by droplet splashes. The air inlet baffle is also provided with concave grooves 405 on both sides. The entire sliding top cover 104 has four concave grooves, which are in the shape of concave triangles. Their size is the same as the triangular cross section of the long slide rail, which can form a concave-convex fit with it to ensure the smooth sliding of the sliding top cover 104 on the slide rail. The top cover handle 403 is located at two corners on the same side of the top cover body and is connected to the small lead screw stepper motor 2 by a connecting rod 3. One end of the connecting rod 3 is attached to the protruding handle and the other end is attached to the small lead screw stepper motor 2. The automatic sliding of the top cover is achieved by the lead screw stepper motor driving the connecting rod 3 to move linearly on the lead screw. At the same time, the protruding handle on one side of the top cover can ensure that the inkjet printing droplets are not affected when they are distributed onto the DNA synthesis chip.

[0064] The aforementioned temperature and humidity control reaction chamber for inkjet-printed enzymatic DNA synthesis includes a small wireless temperature and humidity sensor installed on the inner surface of the upper cover body 401. This sensor monitors the temperature and humidity of the gas inside the reaction chamber in real time, providing temperature and humidity measurement signals for feedback control. The small wireless temperature and humidity sensor is connected to a humidity generator 5. To further detect the temperature and humidity of the gas inside the reaction chamber and facilitate timely feedback and adjustment, the probe of the small wireless temperature and humidity sensor is located inside the reaction area to detect the gas temperature and humidity, providing temperature and humidity measurement signals for feedback control.

[0065] The aforementioned temperature and humidity controlled reaction chamber for inkjet-printed enzymatic DNA synthesis is made of transparent acrylic (alternatively, resin, PEEK, PLA plastic, and other suitable materials can also be used). The chamber's air inlet connection 101 and the first reaction area 102 / second reaction area 103 are completely sealed by clamping a sealing sheet in the middle and then screwing in a screw. Alternatively, any known gap sealing method, including adhesives, pressure, or tightening, can be used for complete sealing. The chamber's air inlet connection 101, the first reaction area 102, the second reaction area 103, and the chip fixing cover 105 all have threaded holes with correspondingly sized sealing sheets. The reaction chamber 1 is assembled by clamping the sealing sheet and tightening the screw. The sealing sheet used is an elastic material, including but not limited to PDMS, silicone, polysilane materials, polyurethane, acrylate, polyesterimide, nitrile rubber, and acrylic rubber.

[0066] The temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis described above has a triangular cross-section on the slide rails on the side cover support walls 302 of the first reaction area 102 and the second reaction area 103 (it can also be any shape that can form a concave-convex fit and be stable, such as a semi-circular arc, elliptical arc, rectangle, trapezoid, serrated shape, etc.); the shape of the arrayed air inlets on the pore array connecting wall 301 can be diversified, including but not limited to one of the following: circle, triangle, square, rectangle, pentagon, hexagon, octagon, other regular polygons, honeycomb.

[0067] The aforementioned temperature and humidity controlled reaction chamber for inkjet-printed enzymatic DNA synthesis can be arranged in multiple series and parallel configurations, allowing for scalability and simultaneous synthesis of more DNA synthesis chips. The reaction chamber operation requires external support, including: a small lead screw stepper motor 2, a connecting rod 3, an inkjet printer 4, a humidity generator 5, and a compressed gas cylinder 6. The linear movement of the small lead screw stepper motor 2 drives the connecting rod 3 to automatically move the sliding cover 104. The inkjet printer 4 precisely dispenses the four nucleotide monomers (A, T, C, G) for enzymatic DNA synthesis, and provides deprotection and cleaning reagents, as well as gas drying. The gas generated by the compressed gas cylinder 6, after passing through the humidity generator 5, carries a certain temperature and humidity. After passing through a gas conduit 202 wrapped with a heating tape, the gas is stably introduced into the reaction chamber 1 by adjusting the cylinder pressure.

[0068] Examples of the dimensions of the components in the temperature and humidity control reaction chamber for inkjet-printed enzymatic DNA synthesis are as follows: The DNA synthesis chip used measures 75mm × 25mm × 1mm (length × width × height).

[0069] The diameter of the gas passage of the two-position three-way solenoid valve 201 in the cabin air intake connection is at least 4 mm or wider, the inner diameter of the gas conduit 202 is at least 3 mm or wider, and the outer diameter of the gas conduit 202 is at least 4 mm or wider.

[0070] The intake chamber 203 is 55mm long, 90mm wide, and 16mm high. Each wall of the chamber is 2mm thick, and the diameter of the circular hole on the front wall of the chamber is 8mm. The outer edge of the intake connecting plate 204 is 120mm long and 30mm wide, and the rectangular through hole in the middle is 90mm long and 16mm wide.

[0071] The perforated connecting wall 301 is 120mm long, 30mm high, and 10mm thick. Each small hole in the arrayed air inlet has a diameter of 1.5mm, with 27 holes in each horizontal row and a center-to-center distance of 3mm. Each vertical row has 6 holes and a center-to-center distance of 2mm.

[0072] The upper cover supports the side wall 302 with a length of 180mm, a width of 10mm, and a height of 30mm. The slide rail has a length of 180mm and a triangular cross-section with a base length of 10mm and a height of 5mm.

[0073] The chip placement platform 303 has a length of 180mm, a width of 17.5mm, and a height of 10mm. The boss has a length of 180mm, a width of 12.5mm, a protrusion height of 1mm, and a distance of 75mm between the two bosses. The bottom bevel 304 has a length of 180mm, a width of 65mm, and a thickness of 9mm at the connection point with the aperture array connecting wall 301.

[0074] The main body of the top cover 401 has a total length of 260mm. The exhaust port 404 of the top cover is 20mm long, 100mm wide, and 5mm away from both sides of the top cover. The air intake baffle 402 has a lower edge length of 90mm, a left and right edge height of 13mm, and a thickness of 1mm.

[0075] The upper and lower threaded holes on the outer periphery of the intake connecting plate 204 are the same and symmetrical, with 5 or more holes, and the left and right threaded holes are the same and symmetrical, with 3 or more holes.

[0076] The number of threaded holes on the upper and lower sides of the outer periphery of the hole array connecting wall 301 is the same and symmetrical, five or more, and the position is the same as the threaded hole on the air intake connecting plate 204. The number of threaded holes on the left and right sides is the same and symmetrical, three or more, and the position is the same as the threaded hole on the air intake connecting plate 204.

[0077] The diameter of the arrayed air inlets on the perforated connecting wall 301 is at least 0.5 mm, 0.6 mm, 0.7 mm, 0.8 mm, 0.9 mm, 1 mm, 1.1 mm, 1.2 mm, 1.3 mm, 1.4 mm, 1.5 mm or wider.

[0078] The number of arrayed air inlets on the perforated connecting wall 301 is at least 10, 15, 20, 25 or more in the lateral direction, and at least 5, 6, 7, 8, 9, 10 or more in the longitudinal direction.

[0079] The reaction zones 102 / 103 of the chamber can hold one, two, three, four, five or more synthetic chips.

[0080] The relevant threaded hole diameter used must be at least 1mm, 2mm, 3mm, 4mm, 5mm or wider.

[0081] The screw model used must match the threaded hole, and its specification must be at least one of M1, M2, M3, M4, or M5.

[0082] The temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis described above, the overall working process of the reaction chamber is as follows: (1) The initial position of the reaction chamber 1 is: the sliding cover 104 is completely above the first reaction area 102, and the DNA synthesis chip is fixed on the first reaction area 102 / second reaction area 103 by the chip fixing cover 105. The number of DNA synthesis chips can be determined according to the requirements of this synthesis. (2) A synthesis solution containing deoxyribonucleic acid (dNTP) and terminal deoxyribonucleotidyl transferase (TdT enzyme) is printed on the DNA synthesis chip fixed on the second reaction region 103 by inkjet printing device 4. (3) After inkjet printing is completed, the sliding cover 104 slides to the top of the second reaction area 103 under the action of the small lead screw stepper motor 2. At this time, the two-position three-way solenoid valve 201 opens to the side of the second reaction area 103, and guides the gas after passing through the humidity generator 5 to the second reaction area 103. The second reaction area 103 is filled with gas with a certain temperature and humidity to ensure efficient enzymatic DNA synthesis. (4) After the enzymatic DNA synthesis reaction is completed, the sliding cover 104 slides to the top of the first reaction area 102 under the action of the small screw stepper motor 2. Then, the DNA synthesis chip is subjected to the process of deprotection reagent supply, drying, cleaning reagent supply, and drying through the inkjet printing device 4. The waste liquid will flow into the waste liquid tank through the bottom inclined surface 304 to complete one enzymatic DNA synthesis cycle. (5) During the enzymatic DNA synthesis reaction in the second reaction region 103, the inkjet printer 4 can move to the first reaction region 102 to distribute the synthesis solution. By repeating the above steps (1), (2), (3), and (4), the enzymatic DNA synthesis is alternately carried out in the two reaction regions (first reaction region 102 / second reaction region 103).

[0083] In summary, the temperature and humidity controlled reaction chamber device for inkjet printing enzymatic DNA synthesis described in this invention has the following characteristics: ① By designing two reaction regions (first reaction region 102 / second reaction region 103), the two regions can simultaneously and alternately carry out the enzymatic DNA synthesis cycle, which effectively improves the efficiency of time utilization and can synthesize more DNA in the same amount of time. ② By designing a sliding top cover 104, it can cooperate with the inkjet printing device 4. When inkjet printing is performed, the sliding top cover 104 leaves the reaction area (first reaction area 102 / second reaction area 103) under the action of the small lead screw stepper motor 2. When enzymatic DNA synthesis is performed, it will return to the reaction area to control the temperature and humidity environment of the synthesis area. ③ By compressing the gas cylinder 6, a stable gas flow with a stable pressure is generated to achieve stable gas input. After passing through the chamber air inlet connection 101 and the reaction area (first reaction area 102 / second reaction area 103), the gas can be evenly dispersed. Both ensure that the temperature and humidity are uniform and consistent when the gas enters the reaction area. ④ By designing a chip placement platform 303 with reaction regions (first reaction region 102 / second reaction region 103) and using a chip fixing cover 105, the DNA synthesis chip can be stably clamped and the chip can be easily disassembled and installed. ⑤ By designing an air inlet baffle 402 that can completely cover the arrayed air inlets, the air inlets are prevented from being contaminated by splashing droplets during the cleaning and drying process. ⑥ By designing the bottom slope 304 of the reaction area (first reaction area 102 / second reaction area 103), the waste liquid blown during the inkjet printing process can flow smoothly into the waste liquid tank through the gap between the two chips and the slope, which facilitates the collection of waste liquid.

[0084] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A temperature and humidity controlled reaction chamber for enzymatic DNA synthesis in inkjet printing, characterized in that: The system includes a cabin air intake connection, a first reaction area, a second reaction area, a sliding top cover, and a chip fixing cover. The cabin air intake connection is fixed between the first and second reaction areas. The cabin air intake connection includes a two-position three-way solenoid valve, a gas conduit, an air intake chamber, and an air intake connecting plate. A partition is provided in the middle of the air intake chamber, dividing it into a left chamber and a right chamber. The air intake chamber is located between the first and second reaction areas. The left chamber is fixedly connected to the first reaction area via its side air intake connecting plate, and the right chamber receives air through its side. The connecting plate is fixedly connected to the second reaction area. The air intake connecting plate is symmetrically arranged on both sides of the air intake chamber. The two-position three-way solenoid valve includes one air intake channel and two air outlet channels. The three channels are respectively connected to the humidity generator and the left and right chambers of the air intake chamber through gas conduits. The two-position three-way solenoid valve controls the gas from the humidity generator to only connect to one side of the air intake chamber at any given time. The slidable top cover is placed above the chamber air intake connecting part, the first reaction area and the second reaction area and is slidably connected. The chip fixing cover is set in the first reaction area and the second reaction area.

2. The temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis according to claim 1, characterized in that: The main structure of the air intake chamber is a cuboid chamber structure. Two circular holes for connecting gas conduits are opened on the front wall of the chamber. A baffle for buffering the gas is provided in the center of the chamber. The air intake connecting plate is a rectangular plate with a rectangular through hole in the middle. The size of the through hole matches the external size of the air intake chamber. The air intake connecting plate is sealed to the first reaction area / second reaction area by a clamping gasket.

3. The temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis according to claim 1, characterized in that: The intake connecting plate is a separate cuboid structure, installed on both sides of the intake chamber. The rectangular through hole in the middle of the intake connecting plate is the same size as the intake chamber, which facilitates the intake connecting plate to be accurately fitted into both sides of the intake chamber and sealed and fixed. The intake connecting plate is tightly assembled with the first reaction area / second reaction area through clamping gaskets.

4. The temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis according to claim 1, characterized in that: The first reaction area / second reaction area has a scoop-shaped structure, including a perforated connecting wall, an upper cover supporting side wall, and a lower chamber wall composed of a chip placement platform and a bottom slope. The perforated connecting wall is located at one end of the bottom slope of the lower chamber wall. The upper cover supporting side wall is symmetrically arranged on both sides of the bottom slope of the lower chamber wall and fixedly connected to both sides of the perforated connecting wall. At the position of the bottom slope adjacent to the upper cover supporting side wall, a chip placement platform for supporting and fixing the DNA synthesis chip is provided. The thickness at the connection position of the bottom slope and the perforated connecting wall gradually decreases along the direction away from the perforated connecting wall, so that the bottom slope forms a downward sloping angle with the horizontal plane.

5. The temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis according to claim 4, characterized in that: The perforated connecting wall is located at one end of the scoop-shaped structure. The middle of the perforated connecting wall has an array of air inlets arranged in a parallel or cross pattern. Each air inlet has the same diameter, and the center-to-center distance between adjacent air inlets in each horizontal and vertical row is equal.

6. The temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis according to claim 4, characterized in that: The lower wall of the chamber consists of a chip placement platform and a bottom slope. The width of the lower wall is equal to the length of the perforated array connecting wall minus the thickness of the side walls supporting the upper cover. A chip placement platform is positioned on each side of the lower wall adjacent to the side walls supporting the upper cover to support and fix the DNA synthesis chip. A long protrusion is positioned on each of the two chip placement platforms adjacent to the side walls supporting the upper cover, working in conjunction with the chip fixing cover to clamp and secure the DNA synthesis chip, ensuring stability during chamber movement, reagent spraying, and gas purging. Between the two chip placement platforms is a bottom slope, forming a downward angle with the horizontal plane. This ensures that the waste liquid generated by the DNA synthesis reaction can flow smoothly along the bottom slope into a waste liquid tank located below the chamber for collection. The bottom slope is coated with a hydrophobic coating to increase its hydrophobicity, improve waste liquid collection efficiency, and reduce the risk of waste liquid accumulation.

7. The temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis according to claim 1, characterized in that: The chip fixing cover includes two types: rectangular cover and rotatable clamping cover.

8. The temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis according to claim 1, characterized in that: The slidable top cover includes a top cover body, an air inlet baffle, and a top cover handle. The air inlet baffle is fixed on both sides of the top cover body, the top cover handle is located on the upper surface of the top cover body, and the top cover body is provided with a top cover exhaust port.

9. The temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis according to claim 8, characterized in that: A small wireless temperature and humidity sensor is installed on the inner surface of the upper cover body, and the small wireless temperature and humidity sensor is connected to the humidity generator.

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