A device and method for rough marking of shrimp fry based on ectopic culture of microbial floc

Abstract

The present application belongs to the technical field of aquaculture, and particularly relates to a shrimp fry marking device and method based on microbial floc ectopic culture. The device comprises a fry marking tank, a heterotrophic microbial purification tank and a carbon source adding device. The inside of the multi-hole anti-escape vertical pipe is communicated with a gravity self-flowing drainage pipe. The gravity self-flowing drainage pipe flows into a floc anti-escape net frame of the heterotrophic microbial purification tank. A circulation pump water inlet pipe is connected between the bottom of the purification tank body and the inner floc anti-escape net frame. Water is sent into the fry marking tank body through the circulation pump. The carbon source adding device is communicated with the inside of the floc anti-escape net frame. Carbon source solution is added into the floc anti-escape net frame. The present application can automatically culture and manage the floc biomass according to daily feeding conditions and brush cleaning frequency, and control the water quality of the fry marking tank at a safe concentration.

Description

Technical Field

[0001] This invention belongs to the field of aquaculture technology, specifically a shrimp larvae raising device and method based on ex-situ culture of microbial flocs. Background Technology

[0002] The rearing process of shrimp larvae mainly employs different techniques, including algal culture, flocculent culture (mixed phase), bacterial culture, and water-change culture. If the first three techniques are properly controlled, water consumption can be significantly reduced, and the rearing process can even be completed without water changes. In recent years, there has been an increasing number of cases of artificial seawater shrimp farming in inland areas of my country, and production problems have arisen accordingly. Especially in inland plateau regions such as Yunnan and Guizhou, due to low atmospheric oxygen levels and high costs of preparing artificial seawater, achieving efficient shrimp larvae cultivation and completing the rearing stage with minimal or no water changes presents a significant challenge.

[0003] The cultivation of shrimp larvae using algal or mixed algal-bacterial floc culture methods is easily affected by cloudy weather and other climatic conditions. Continuous cloudy days can easily cause algal death, resulting in algal toxins that affect the survival rate of shrimp larvae. Water exchange-based aquaculture not only has high heating energy consumption but also high water costs. Therefore, using a microbial culture method for shrimp larvae rearing has become the preferred choice for cost reduction and efficiency improvement.

[0004] Traditional microbial biofloc culture involves adding a balanced blend of carbon sources, heterotrophic bacteria, feed, vitamins, and other components to the water used for shrimp larvae rearing to maintain stable levels of nutrients, ammonia nitrogen, and nitrite. However, this method carries the risk that the rapid growth of the heterotrophic microbial flocs leads to a rapid increase in oxygen consumption. In high-altitude areas where dissolved oxygen levels are already low (saturated dissolved oxygen < 5.5 mg / L), maintaining dissolved oxygen within the optimal range for shrimp larvae growth becomes difficult. Furthermore, the increase in biomass also increases oxygen-consuming organic matter in the water, limiting the stocking density of shrimp larvae and necessitating premature termination of the stocking process, thus impacting overall aquaculture efficiency. Summary of the Invention

[0005] In order to solve the above-mentioned problems in the traditional microbial biofloc culture method for shrimp larvae raising, the present invention aims to provide a shrimp larvae raising device and method based on ex-situ culture of microbial flocs.

[0006] The objective of this invention is achieved through the following technical solution:

[0007] The shrimp larvae rearing device of the present invention includes a rearing pond, a heterotrophic microbial purification pond, and a carbon source addition device;

[0008] The seedling tank includes an automatic feeder, a pressure water pump, a self-moving flushing pipe, an escape-proof net A, a porous escape-proof riser, an aeration disc A, the seedling tank body, a sewage valve, and a gravity-fed drainage pipe. The porous escape-proof riser is installed inside the seedling tank body, and the escape-proof net A covers the porous escape-proof riser. A self-moving flushing pipe, capable of relative lifting and rinsing the escape-proof net A and the porous escape-proof riser, is fitted onto the porous escape-proof riser covering the escape-proof net A. The self-moving flushing pipe is connected to a water source via the pressure water pump. An aeration disc A is located at the bottom of the seedling tank body, surrounding the porous escape-proof riser. An automatic feeder is located above the seedling tank body, and the interior of the porous escape-proof riser is connected to a gravity-fed drainage pipe.

[0009] The heterotrophic microbial purification tank includes a circulating pump, a variable frequency motor, a scrubbing device, a floc collection pipe, an aeration disc B, a floc escape prevention net frame, and a purification tank body. The floc escape prevention net frame is installed inside the purification tank body and has an escape prevention net B on it to intercept flocs in the aquaculture water. The interior of the porous escape prevention riser is connected to the interior of the floc escape prevention net frame via a gravity-fed drainage pipe. A variable frequency motor is installed on the purification tank body, and the output end of the variable frequency motor is connected to the scrubbing device located inside the floc escape prevention net frame, driving the scrubbing device to scrub the floc escape prevention net frame. An aeration disc B is located at the bottom of the floc escape prevention net frame, and excess biological flocs are collected through a floc collection pipe connected to the bottom of the floc escape prevention net frame. The circulating pump's inlet pipe is connected to the interior of the purification tank body, and the circulating pump's outlet pipe is connected to the interior of the seedling tank body.

[0010] The carbon source adding device is connected to the inside of the floc escape prevention net frame, and adds carbon source solution into the floc escape prevention net frame.

[0011] The self-moving flushing pipe is divided into two groups, upper and lower, connected by connecting pipes. Each group includes a flushing pipe, a stainless steel ring, and an annular brush A. The outer ring of the flushing pipe is connected to the stainless steel ring as a counterweight, and the inner ring of the flushing pipe is connected to the brush A. The upper and lower flushing pipes are connected by multiple connecting pipes. The bottom surface of the flushing pipe has multiple water outlet holes evenly opened along the circumferential direction. The axial center line of the water outlet holes is inclined to the axial center line of the flushing pipe. The flushing pipe located at the top is connected to the pressure water pump.

[0012] The carbon source addition device includes a carbon source solution addition pump, a carbon source adder, and a carbon source storage tank. The carbon source storage tank is filled with seawater, and a carbon source adder is provided above the carbon source storage tank. The inlet pipe of the carbon source solution addition pump is connected to the inside of the carbon source storage tank, and the outlet pipe of the carbon source solution addition pump is connected to the inside of the floc escape prevention net frame.

[0013] The bottom of the carbon source storage tank is equipped with an aeration disc C to promote carbon source dissolution. The carbon source storage tank is also equipped with a liquid level sensor. The liquid level sensor, carbon source additive, carbon source solution return pump and automatic feeder are respectively connected to the controller. The carbon source storage tank is equipped with a carbon source storage tank water supply pipe for replenishing seawater into the tank.

[0014] The seedling tank is equipped with a pressure water pump level switch connected to a pressure water pump. The pressure water pump has two modes: timed start and start controlled by the pressure water pump level switch.

[0015] The purification tank is equipped with a liquid level switch. The liquid level switch and the variable frequency motor are respectively connected to the controller. The variable frequency motor has two modes: timed start and liquid level switch controlled start.

[0016] The brushing device includes a rotating shaft and brushes B. The rotating shaft is connected to the output end of a variable frequency motor. Multiple brushes B are connected axially on the rotating shaft. The variable frequency motor drives the rotating shaft to rotate, thereby driving the multiple brushes B to brush the anti-escape net B of the clump anti-escape net frame.

[0017] One end of the floc collection pipe is connected to the bottom of the floc escape prevention net frame, and the other end of the floc collection pipe is connected to a solenoid valve; the circulating pump inlet pipe is connected to the bottom of the space between the purification tank and the floc escape prevention net frame.

[0018] The bottom of the seedling pool slopes downward from the outside to the inside. The gravity-fed drainage pipe is divided into three paths: the first path is connected to the lowest point of the bottom of the seedling pool, the second path is connected to the inside of the floc anti-escape net frame, and the third path is equipped with a sewage discharge valve. The circulating pump outlet pipe is connected to the top of the seedling pool, and a water supply pipe is also connected to the top of the seedling pool.

[0019] The present invention relates to a method for using a shrimp larvae raising device based on ex-situ culture of microbial flocs, comprising the following steps:

[0020] Step A: Before introducing the seedlings, add water to the purification tank to the set water level, add probiotics and carbon source to the floc escape prevention net frame, and aerate through the aeration disc B to make the flocs grow and form granular flocs.

[0021] Step B: After the shrimp larvae are placed into the seedling tank, they are fed by the automatic feeder. The circulation pump is turned on to pump the water between the floc escape prevention net frame and the purification tank into the seedling tank. The water in the porous escape prevention riser flows into the floc escape prevention net frame through the gravity drainage pipe.

[0022] Step C: When the escape-proof net A on the porous escape-proof riser becomes clogged, causing the water level in the seedling pond to rise, the pressure water pump is manually or automatically turned on to pump water into the self-moving flushing pipe connected to the pressure water pump. The dirt on the escape-proof net A and the porous escape-proof riser is cleaned by the up-and-down movement of the self-moving flushing pipe.

[0023] Step D: Based on the daily feeding amount of the automatic feeder, the brushing device is activated by the frequency converter at set time intervals.

[0024] Step E: Add carbon source solution into the floc anti-escape net frame according to the daily feeding amount of the automatic feeder;

[0025] Step F: Control floc biomass. Assess floc biomass through manual inspection or by the frequency of cleaning the floc escape prevention net frame. Collect excess bio-flocs using the floc collection pipe. The discharged bio-flocs can be collected by static sedimentation and then filtered for use as feed additives or bio-fertilizers.

[0026] The advantages and positive effects of this invention are as follows:

[0027] 1. This invention has a high degree of automation. It achieves water recycling through a circulating pump, saving more than 90% of water compared to the traditional seedling method, and significantly reducing heating energy consumption.

[0028] 2. The present invention provides in-situ purification treatment of aquaculture wastewater, with no aquaculture wastewater discharge.

[0029] 3. The bioflocs produced by this invention are good probiotics that can be used for water environment regulation in aquaculture or as a protein additive in feed. Attached Figure Description

[0030] Figure 1 This is a schematic diagram of the overall structure of the shrimp larvae raising device of the present invention;

[0031] Figure 2 This is a bottom view of the structure of the self-moving flushing pipe and the porous escape-proof riser in the shrimp larvae raising device of the present invention.

[0032] Figure 3 This is a front view of the structure of the self-moving flushing pipe and the porous escape-proof riser in the shrimp larvae raising device of the present invention.

[0033] Wherein: 1 is the seedling pond, 101 is the automatic feeder, 102 is the pressure water pump, 103 is the pressure water pump inlet pipe, 104 is the self-moving flushing pipe, 1041 is the flushing pipe, 1042 is the water outlet, 1043 is the stainless steel ring, 1044 is the brush, 1045 is the rubber at the bottom of the brush, 1046 is the connecting pipe, 105 is the water replenishment pipe, 106 is the escape-proof net A, 107 is the multi-hole escape-proof riser, 108 is the dotted aeration disc A, 109 is the seedling pond body, 110 is the sewage valve, 111 is the gravity-fed drainage pipe, 112 is the pressure water pump level switch, and 113 is the air inlet pipe A.

[0034] 2 is a heterotrophic microbial purification tank; 201 is a circulating pump; 202 is a circulating pump inlet pipe; 203 is a circulating pump outlet pipe; 204 is a variable frequency motor; 205 is a liquid level switch; 206 is a fixed bracket; 207 is a brush B; 208 is a solenoid valve; 209 is a rotating shaft; 210 is a floc collection pipe; 211 is an air inlet pipe B; 212 is a dotted aeration disc B; 213 is a floc escape prevention net frame; 214 is the purification tank body.

[0035] 3 is a carbon source addition device, 301 is a controller, 302 is a carbon source solution addition pump, 303 is a carbon source adder, 304 is the inlet pipe of the carbon source solution addition pump, 305 is a liquid level sensor, 306 is a carbon source solution addition pipe, 307 is a water supply pipe for the carbon source storage tank, 308 is a carbon source storage tank, and 309 is a dotted aeration disc C. Detailed Implementation

[0036] The invention will now be described in further detail with reference to the accompanying drawings.

[0037] like Figure 1 As shown, the shrimp larvae rearing device of the present invention includes a rearing pond 1, a heterotrophic microbial purification pond 2, and a carbon source addition device 3.

[0038] Nursery 1 includes an automatic feeder 101, a pressure water pump 102, a self-propelled flushing pipe 104, an escape-proof net A106, a porous escape-proof riser 107, an aeration disc A, a nursery body 109, a drain valve 110, and a gravity-fed drainage pipe 111. The porous escape-proof riser 107 is installed inside the nursery body 109, and the escape-proof net A106 covers the outside of the porous escape-proof riser 107. A self-moving flushing pipe 104 is installed, which can be raised and lowered to flush the escape-proof net A106 and the porous escape-proof riser 107. The self-moving flushing pipe 104 is connected to the water source through a pressure water pump 102. An aeration disc A is provided at the bottom of the seedling tank 109, located around the porous escape-proof riser 107. An automatic feeder 101 is provided above the seedling tank 109. A gravity-fed drainage pipe 111 is connected inside the porous escape-proof riser 107.

[0039] The heterotrophic microbial purification tank 2 includes a circulating pump 201, a variable frequency motor 204, a scrubbing device, a floc collection pipe 210, an aeration disc B, a floc escape prevention net frame 213, and a purification tank body 214. The floc escape prevention net frame 213 is installed inside the purification tank body 214, and is equipped with an escape prevention net B to intercept flocs in the aquaculture water. The interior of the porous escape prevention riser 107 is connected to the interior of the floc escape prevention net frame 213 through a gravity-flow drainage pipe 111. The variable frequency motor 204 is installed on the purification tank body 214, and the output end of the variable frequency motor 204 is connected to the floc escape prevention net frame 213. The internal brushing device is connected to and drives the brushing device to brush the floc escape prevention net frame 213; the bottom of the floc escape prevention net frame 213 is equipped with an aeration disc B, and excess biological flocs are collected through the floc collection pipe 210 connected to the bottom of the floc escape prevention net frame 213; the circulation pump inlet pipe 202 of the circulation pump 201 is connected to the inside of the purification tank 214, specifically to the bottom of the space between the purification tank 214 and the floc escape prevention net frame 213, and the circulation pump outlet pipe 203 of the circulation pump 201 is connected to the inside of the seedling tank 109, and water flows into the seedling tank 109 through the circulation pump 201.

[0040] The carbon source addition device 3 includes a carbon source solution addition pump 302, a carbon source adder 303, and a carbon source storage tank 308. The carbon source storage tank 308 is filled with seawater. The carbon source adder 303 is located above the carbon source storage tank 308. The carbon source solution addition pump inlet pipe 304 of the carbon source solution addition pump 302 is connected to the inside of the carbon source storage tank 308. The carbon source solution addition pump outlet pipe 306 of the carbon source solution addition pump 302 is connected to the inside of the floc escape prevention net frame 213, and carbon source solution is added into the floc escape prevention net frame 213.

[0041] In this embodiment, the porous escape-proof riser 107 is a hollow round tube with openings at both ends. Multiple perforations are provided along the circumference of the round tube. An escape-proof net A106 is wrapped around the outside of the perforations. In this embodiment, the escape-proof net A106 has a mesh count of 20.

[0042] like Figures 1-3As shown, the self-moving flushing pipe 104 of this embodiment is divided into upper and lower groups connected by a connecting pipe 1046. Each group includes a flushing pipe 1041, a stainless steel ring 1043, and a brush A1044. The flushing pipe 1041 is a circular annular pipe made of PVC material. The outer ring of the flushing pipe 1041 is connected to the stainless steel ring 1043 as a counterweight, and the inner ring of the flushing pipe 1041 is connected to the brush A1044. In this embodiment, the brush A1044 is circular, and the outer ring of the brush A1044 is a brush bottom rubber 1045. The brush bottom rubber 1045 is fixed to the inner ring of the flushing pipe 1041, and the inner ring of the brush A1044 is composed of brush bristles. The stainless steel ring 1043 of this embodiment weighs 500g. The bottom surface of the flushing pipe 1041 is evenly provided with multiple water outlet holes 1042 along the circumferential direction. The axial center line of the water outlet hole 1042 is inclined to the axial center line of the flushing pipe 1041. In this embodiment, the inclination angle between the axial center line of the water outlet hole 1042 and the axial center line of the flushing pipe 1041 is 45°. The diameter of the water outlet hole 1042 is 2mm, and the interval between two adjacent water outlet holes 1042 is 2-3mm. The flushing pipe 1041 located at the top is connected to the pressure water pump 102 to realize automatic cleaning and maintain smooth water flow. The upper and lower sets of flushing pipes 1041 are connected by multiple connecting pipes 1046.

[0043] In this embodiment, the bottom of the seedling tank 109 slopes downwards from the outside to the inside. The gravity-fed drainage pipe 111 is divided into three paths: the first path connects to the lowest point of the bottom of the seedling tank 109, the second path connects to the inside of the floc accumulating prevention net frame 213, and the third path is equipped with a drain valve 110. The circulating pump outlet pipe 203 is connected to the seedling tank 109 near the top, and a water supply pipe 105 is also connected to the seedling tank 109 near the top.

[0044] In this embodiment, the inlet of the pressure water pump 102 is connected to the water source through the pressure water pump inlet pipe 103, and the outlet of the pressure water pump 102 is connected to the flushing pipe 1041 located above through a flexible hose. A pressure water pump level switch 112 connected to the pressure water pump 102 is installed inside the seedling tank 109. The pressure water pump level switch 112 can be fixed to the seedling tank 109. The pressure water pump 102 has two modes: timed activation and activation controlled by the pressure water pump level switch 112. After the pressure water pump 102 is activated, the self-moving flushing pipe 104 rises under the reaction force of the water outlet 1042, cleaning the escape-proof net A106 and the porous escape-proof vertical net 107 during the rising process. One cleaning cycle is completed by adjusting the single activation time of the pressure water pump 102. After cleaning, the self-moving flushing pipe 104 returns to the bottom of the porous escape-proof vertical net 107 under the action of gravity.

[0045] The brushing device in this embodiment includes a rotating shaft 209 and brushes B207. A fixed bracket 206 is installed on the top of the purification tank 214. A variable frequency motor 204 is fixedly connected to the fixed bracket 206. The rotating shaft 209 is connected to the output end of the variable frequency motor 204. Multiple brushes B207 are connected axially on the rotating shaft 209. The variable frequency motor 204 drives the rotating shaft 209 to rotate, thereby driving the multiple brushes B207 to brush the anti-escape net B of the floc anti-escape net frame 213. In this embodiment, the brushes B207 are horizontally comb-shaped brushes, that is, each brush B207 is perpendicular to the rotating shaft 209 and has a comb-shaped outer edge.

[0046] In this embodiment, the escape-prevention net B has a mesh size of 100. The floc escape-prevention net frame 213 and the escape-prevention net B trap flocs in the aquaculture water, achieving ex-situ cultivation of biological flocs. A variable frequency motor 204 drives the rotating shaft 209 to rotate, which in turn drives each brush B207 to rotate. The brushes B207 clean the floc escape-prevention net frame 213 and the escape-prevention net B, ensuring smooth water circulation.

[0047] In this embodiment, a liquid level switch 205 is fixed on a fixed bracket 206 inside the purification tank 214. The liquid level switch 205 and the variable frequency motor 204 are respectively connected to the controller 301. The variable frequency motor 204 has two modes: timed opening and liquid level switch 205 controlled opening. According to the daily feeding amount of the automatic feeder 101, the variable frequency motor 204 can be opened at a time or controlled by the liquid level switch 205 to realize the automatic cleaning of the escape-proof net B on the floc escape-proof net frame 213.

[0048] In this embodiment, one end of the floc collection pipe 210 is connected to the bottom of the floc escape prevention net frame 213, and the other end of the floc collection pipe 210 is connected to a solenoid valve 208. The automatic control of floc biomass is achieved by estimating the biomass through the opening frequency of the liquid level switch 205, combined with the opening of the solenoid valve 208.

[0049] In this embodiment, the bottom of the carbon source storage tank 308 is equipped with an aeration disc C to promote carbon source dissolution. The carbon source storage tank 308 also contains a level sensor 305. The level sensor 305, carbon source additive 303, carbon source solution return pump 302, and automatic feeder 101 are all connected to the controller 301. The carbon source storage tank 308 is equipped with a carbon source storage tank water supply pipe 307 for replenishing seawater. Automatic adjustment of the carbon source solution is achieved by the level sensor 205 sensing the remaining water volume and automatically replenishing water through the water supply pipe 307, in conjunction with the carbon source additive 303. Automatic replenishment of the carbon source solution is achieved by estimating the floc biomass through the operating frequency of the variable frequency motor 204, and simultaneously combining this with the daily feeding amount recorded by the automatic feeder 101. The controller 301 then controls the carbon source solution addition pump 302 to replenish the carbon source solution to the purification tank 214. The carbon source solution is automatically replenished by adding 50-80% of the daily feed weight of the automatic feeder 101. Under the same feed weight conditions, the amount of carbon source solution added is increased as the operating frequency of the variable frequency motor 204 increases. In this embodiment, the carbon source can be glucose, sucrose, or molasses. The seawater in the carbon source storage tank 308 is 400L, and the carbon source mass ratio is 100g carbon source / L water.

[0050] In this embodiment, aeration disc A is a dot-distribution aeration disc A108, aeration disc B is a dot-distribution aeration disc B212, and aeration disc C is a dot-distribution aeration disc C309. The carbon source replenishment device 303 in this embodiment is prior art and can be a motor and an auger, enabling the addition of carbon source to the carbon source storage tank 308.

[0051] The present invention relates to a method for using a shrimp larvae raising device based on ex-situ culture of microbial flocs, comprising the following steps:

[0052] Step A: Three days before introducing the seedlings, add water to the purification tank 214 to the set water level, add probiotics and carbon source to the floc escape prevention net frame 213, and aerate through the aeration disc B to make the flocs grow rapidly and form granular flocs; the probiotics can be Bacillus or EM bacteria, add 30-50g of probiotics each time, and add 50-80% of the daily feed weight of the carbon source automatic feeder 101;

[0053] Step B: After the shrimp larvae are placed in the seedling tank 109, the shrimp larvae are fed by the automatic feeder 101. The circulation pump 201 is turned on to pump the water between the floc escape prevention net frame 213 and the purification tank 214 into the seedling tank 109. The water in the porous escape prevention riser 107 flows into the floc escape prevention net frame 213 through the gravity flow drain pipe 111.

[0054] Step C: When the escape-proof net A106 on the porous escape-proof riser 107 becomes clogged, causing the water level in the seedling pool 109 to rise, the pressure water pump 102 is manually or automatically turned on to pump water into the self-moving flushing pipe 104 connected to the pressure water pump 102. The escape-proof net A106 and the dirt on the porous escape-proof riser 107 are cleaned by the up and down movement of the self-moving flushing pipe 104.

[0055] Step D: Based on the daily feeding amount of the automatic feeder 101, the brush B207 is activated by the frequency converter 204 at set time intervals. The timed activation principle of the frequency converter 204 is based on the working conditions of the automatic feeder 101: once every 6 hours when the daily feeding amount is 0-300g, once every 3 hours when the daily feeding amount is 300-600g, once every 1 hour when the daily feeding amount is 600-900g, once every 30 minutes when the daily feeding amount is 900-1200g, once every 20 minutes when the daily feeding amount is 1200-1500g, and once every 10 minutes when the daily feeding amount is 1500-1800g. The duration of each activation is not less than 5 seconds.

[0056] Step E: Add carbon source solution to the floc anti-escape net frame 213 at 50-80% of the daily feeding mass of the automatic feeder 101. Under the same feeding conditions, increase the carbon source addition mass as the frequency of the variable frequency motor 204 increases.

[0057] Step F: Control floc biomass. Assess floc biomass through manual inspection or by the frequency of cleaning the floc escape prevention net frame 213. Collect excess bio-flocs using the floc collection pipe 210. The discharged bio-flocs can be collected by static sedimentation and then collected by pressure filtration for use as feed additives or bio-fertilizers.

Claims

1. A shrimp larvae rearing device based on ex-situ culture of microbial flocs, characterized in that: It includes a seedling pond (1), a heterotrophic microbial purification pond (2), and a carbon source addition device (3); The seedling tank (1) includes an automatic feeder (101), a pressure water pump (102), a self-moving flushing pipe (104), an escape-proof net A (106), a porous escape-proof riser (107), an aeration disc A, a seedling tank body (109), a sewage valve (110), and a gravity-fed drainage pipe (111). The porous escape-proof riser (107) is installed inside the seedling tank body (109), and the escape-proof net A (106) covers the porous escape-proof riser (107). The porous escape-proof riser (104) covered by the escape-proof net A (106) is located inside the seedling tank body (109). 7) A self-moving flushing pipe (104) is fitted on the upper part, which can be raised and lowered to flush the escape-proof net A (106) and the porous escape-proof riser (107). The self-moving flushing pipe (104) is connected to the water source through a pressure water pump (102). An aeration disc A is provided at the bottom of the seedling pool (109) and around the porous escape-proof riser (107). An automatic feeder (101) is provided above the seedling pool (109). A gravity-fed drainage pipe (111) is connected inside the porous escape-proof riser (107). The heterotrophic microbial purification tank (2) includes a circulating pump (201), a variable frequency motor (204), a scrubbing device, a floc collection pipe (210), an aeration disc B, a floc escape prevention net frame (213), and a purification tank body (214). The floc escape prevention net frame (213) is installed inside the purification tank body (214). The floc escape prevention net frame (213) is equipped with an escape prevention net B for intercepting flocs in the aquaculture water. The interior of the porous escape prevention riser (107) is connected to the interior of the floc escape prevention net frame (213) through a gravity-fed drainage pipe (111). The purification tank body (214) is equipped with a variable frequency motor (201). 04), the output end of the variable frequency motor (204) is connected to the brushing device located in the floc escape prevention net frame (213) and drives the brushing device to brush the floc escape prevention net frame (213); the bottom of the floc escape prevention net frame (213) is provided with an aeration disc B, and excess biological flocs are collected through the floc collection pipe (210) connected to the bottom of the floc escape prevention net frame (213); the circulation pump inlet pipe (202) of the circulation pump (201) is connected to the inside of the purification tank (214), and the circulation pump outlet pipe (203) of the circulation pump (201) is connected to the inside of the seedling tank (109); The carbon source adding device (3) is connected to the inside of the floc escape prevention net frame (213) and adds carbon source solution into the floc escape prevention net frame (213); The self-moving flushing pipe (104) is divided into two groups, upper and lower, connected by connecting pipes (1046). Each group includes a flushing pipe (1041), a stainless steel ring (1043), and an annular brush A (1044). The outer ring of the flushing pipe (1041) is connected to the stainless steel ring (1043) as a counterweight. The inner ring of the flushing pipe (1041) is connected to the brush A (1044). The upper and lower flushing pipes (1041) are connected by multiple connecting pipes (1046). The bottom surface of the flushing pipe (1041) is evenly provided with multiple water outlet holes (1042) along the circumferential direction. The axial center line of the water outlet hole (1042) is inclined to the axial center line of the flushing pipe (1041). The flushing pipe (1041) located above is connected to the pressure water pump (102). The seedling pool (109) is equipped with a pressure water pump level switch (112) connected to the pressure water pump (102). The pressure water pump (102) has two modes: timed start and start controlled by the pressure water pump level switch (112).

2. The shrimp larvae raising device based on ex-situ culture of microbial flocs according to claim 1, characterized in that: The carbon source addition device (3) includes a carbon source solution addition pump (302), a carbon source adder (303), and a carbon source storage tank (308). The carbon source storage tank (308) is filled with seawater. The carbon source adder (303) is provided above the carbon source storage tank (308). The carbon source solution addition pump inlet pipe (304) of the carbon source solution addition pump (302) is connected to the inside of the carbon source storage tank (308). The carbon source solution addition pump outlet pipe (306) of the carbon source solution addition pump (302) is connected to the inside of the floc escape prevention net frame (213).

3. The shrimp larvae raising device based on ex-situ culture of microbial flocs according to claim 2, characterized in that: The bottom of the carbon source storage tank (308) is provided with an aeration disc C to promote the dissolution of carbon source. The carbon source storage tank (308) is also provided with a liquid level sensor (305). The liquid level sensor (305), carbon source additive (303), carbon source solution return pump (302) and automatic feeder (101) are respectively connected to the controller (301). The carbon source storage tank (308) is provided with a carbon source storage tank water supply pipe (307) for replenishing seawater into the tank.

4. The shrimp larvae raising device based on ex-situ culture of microbial flocs according to claim 1, characterized in that: The purification tank (214) is equipped with a liquid level switch (205). The liquid level switch (205) and the variable frequency motor (204) are respectively connected to the controller (301). The variable frequency motor (204) has two modes: timed start and start controlled by the liquid level switch (205).

5. The shrimp larvae raising device based on ex-situ culture of microbial flocs according to claim 1, characterized in that: The brushing device includes a rotating shaft (209) and brushes B (207). The rotating shaft (209) is connected to the output end of a variable frequency motor (204). Multiple brushes B (207) are connected axially on the rotating shaft (209). The variable frequency motor (204) drives the rotating shaft (209) to rotate, thereby driving the multiple brushes B (207) to brush the anti-escape net B of the floc anti-escape net frame (213).

6. The shrimp larvae raising device based on ex-situ culture of microbial flocs according to claim 1, characterized in that: One end of the floc collection pipe (210) is connected to the bottom of the floc escape prevention net frame (213), and the other end of the floc collection pipe (210) is connected to a solenoid valve (208); the circulating pump inlet pipe (202) is connected to the bottom of the space between the purification tank (214) and the floc escape prevention net frame (213).

7. The shrimp larvae raising device based on ex-situ culture of microbial flocs according to claim 1, characterized in that: The bottom of the seedling pool (109) slopes downward from the outside to the inside. The gravity-fed drainage pipe (111) is divided into three paths. The first path is connected to the lowest point of the bottom of the seedling pool (109). The second path is connected to the inside of the floc escape prevention net frame (213). The third path is equipped with a sewage valve (110). The circulating pump outlet pipe (203) is connected to the seedling pool (109) near the top. A water supply pipe (105) is also connected to the seedling pool (109) near the top.

8. The method of using the shrimp larvae raising device based on ex-situ culture of microbial flocs according to any one of claims 1 to 7, characterized in that: Includes the following steps Step A: Before introducing the seedlings, add water to the purification tank (214) to the set water level, add probiotics and carbon source to the floc escape prevention net frame (213), and aerate through the aeration disc B to make the flocs grow and form granular flocs. Step B: After the shrimp larvae are placed in the seedling tank (109), the shrimp larvae are fed by the automatic feeder (101). The circulation pump (201) is turned on to pump the water between the floc escape prevention net frame (213) and the purification tank (214) into the seedling tank (109). The water in the porous escape prevention riser (107) flows into the floc escape prevention net frame (213) through the gravity self-flow drainage pipe (111). Step C: When the escape-proof net A (106) on the porous escape-proof riser (107) is blocked, causing the water level in the seedling pool (109) to rise, the pressure water pump (102) is turned on manually or automatically to pump water into the self-moving flushing pipe (104) connected to the pressure water pump (102). The cleanup of dirt on the escape-proof net A (106) and the porous escape-proof riser (107) is achieved by the up and down movement of the self-moving flushing pipe (104). Step D: Based on the daily feeding amount of the automatic feeder (101), the brushing device is activated by the variable frequency motor (204) at set time intervals. Step E: Add carbon source solution into the floc escape prevention net frame (213) according to the daily feeding amount of the automatic feeder (101); Step F: Control floc biomass. Assess floc biomass by manual inspection or by the frequency of cleaning the floc anti-escape net frame (213). Collect excess flocs using the floc collection pipe (210). The discharged flocs can be collected by static sedimentation and then collected by pressure filtration for use as feed additives or bio-fertilizers.

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