A bio-enzyme preparation cleaning method and system

Abstract

The application discloses a kind of biological enzyme preparation cleaning method and system, method includes the following steps: step 1, composite enzyme premix tank is added with glucose and is prepared into mixed solution, and is sprayed into granulating dryer, composite enzyme premix tank contains glucose oxidase, to make glucose and glucose oxidase react under aerobic condition to produce hydrogen peroxide, and make hydrogen peroxide with to the inside of granulating dryer is disinfected and sterilized treatment, step 2, catalase solution in catalase tank is sprayed into the inside of granulating dryer, to carry out the second spray washing of granulating dryer, to make catalase effectively decompose hydrogen peroxide;Step 3, alkaline protease solution in alkaline protease tank is sprayed into the inside of granulating dryer, to carry out the third spray washing of granulating dryer;Step 4, reverse osmosis water in reverse osmosis water tank is sprayed into the inside of granulating dryer, to carry out the fourth spray washing of granulating dryer.The application aims at realizing high-efficiency cleaning granulating dryer.

Description

Technical Field

[0001] This invention relates to the field of equipment cleaning technology, and in particular to a method and system for cleaning with biological enzymes. Background Technology

[0002] Currently, granulation dryers are widely used in the pharmaceutical and food industries. Their complex internal structure makes them prone to retaining contaminants such as sugars, oils, and proteins. Traditional cleaning methods rely on manual operation and use single alkaline proteases or chemical agents. Existing liquid enzyme mixtures often contain proteases, but these proteases decompose other biological enzymes (such as lipases and amylases), leading to the inactivation of the complex enzyme cleaning system and low cleaning efficiency. Current enzyme cleaning methods can only decompose organic matter and cannot kill bacteria, spores, and other microorganisms, easily resulting in excessive total bacterial counts in the product and a lack of sterilization function. Furthermore, the internal piping of granulation dryers is convoluted, and the nozzles are densely distributed. Traditional low-pressure, fixed-angle nozzles cannot cover dead corners such as the collection chamber and base, leaving cleaning blind spots. The cleaning process requires manual reagent preparation, pipeline switching, and time monitoring, which is time-consuming, prone to oversights, and results in poor cleaning consistency, making it highly dependent on manual operation.

[0003] Existing patent CN109234045A discloses a medical multi-enzyme cleaning solution and its preparation method. This cleaning solution uses a combination of components to allow proteases and other enzymes such as lipases, catalases, ligninases, and glucose oxidases to coexist, while reducing the overall enzyme usage and lowering costs. Although surfactants (such as polyoxypropylene glycerol ether and glycerol polyoxypropylene ether) and stabilizers (pullulan) inhibit the decomposition of other enzymes by the proteases, enzyme activity may gradually decrease during long-term storage due to oxidation, temperature changes, or microbial contamination. Furthermore, this solution primarily targets medical contaminants such as blood and human secretions; its cleaning effect on other common industrial contaminants such as oils, sugars, and proteins has not been clearly verified, potentially limiting its applicability. Summary of the Invention

[0004] The main objective of this invention is to provide a method and system for cleaning biological enzyme preparations, aiming to solve the technical problem that existing cleaning methods cannot efficiently clean granulation dryers.

[0005] To achieve the above objectives, the present invention provides a method for cleaning biological enzyme preparations. The method is applied to a biological enzyme preparation cleaning system, the system comprising a granulation dryer and a biological enzyme preparation tank connected to the granulation dryer. The granulation dryer is connected to the biological enzyme preparation tank via a main pipeline. The biological enzyme preparation tank includes a compound enzyme premix tank, a catalase tank, an alkaline protease tank, and a reverse osmosis water tank. The compound enzyme premix tank, catalase tank, alkaline protease tank, and reverse osmosis water tank are each connected to the main pipeline. The method includes the following steps:

[0006] Step 1: Add glucose to the compound enzyme premix tank and prepare a mixed solution. Spray the mixed solution into each area inside the granulator dryer through the main pipeline for the first spray cleaning of the granulator dryer. This allows the glucose and glucose oxidase inside the granulator dryer to react and produce hydrogen peroxide, which can then disinfect and sterilize the inside of the granulator dryer. The activity ratio of liquid amylase, cellulase, lipase, and glucose oxidase in the compound enzyme premix tank is (1-2):(1-2):(3-5):1, and the ratio of the total mass of the mixed solution of amylase, cellulase, lipase, and glucose oxidase to the mass of glucose is (8-10):1.

[0007] Step 2: The catalase solution in the catalase tank is sprayed into various areas inside the granulator dryer through the main pipeline to perform a second spraying of the granulator dryer so that the catalase can effectively decompose hydrogen peroxide. The ratio of the activity of the catalase solution in the catalase tank to that of glucose oxidase is (20-40):3.

[0008] Step 3: The alkaline protease solution in the alkaline protease tank is sprayed into various areas inside the granulator dryer through the main pipeline for the third spray washing of the granulator dryer. The activity ratio of the alkaline protease solution to the catalase solution is 3:(2-4).

[0009] Step 4: Reverse osmosis water from the reverse osmosis water tank is sprayed into various areas inside the granulator dryer through the main pipeline to perform the fourth spray washing of the granulator dryer.

[0010] Optionally, the granulation dryer includes, from top to bottom, a collection chamber, a granulation chamber, and a base, wherein the collection chamber, the granulation chamber, and the base are respectively connected to the biological enzyme preparation tank through a main pipeline.

[0011] Optionally, a first branch pipe, a second branch pipe, and a third branch pipe are respectively arranged between the collection chamber, the granulation chamber, and the base and the main pipeline, and a rotating nozzle is installed at the end of the first branch pipe, the second branch pipe, and the third branch pipe.

[0012] Optionally, the compound enzyme premixing tank, catalase tank, alkaline protease tank, and reverse osmosis water tank are equipped with corresponding first, second, third, and fourth switching valves between themselves and the main pipeline. Each of the first, second, third, and fourth switching valves is connected to the PLC system electrical signal.

[0013] Optionally, a booster pump is also installed in the main pipeline, and the booster pump is electrically connected to the PLC system.

[0014] Optionally, the first branch pipe, the second branch pipe, and the third branch pipe are also equipped with corresponding first regulating valves, second regulating valves, and third regulating valves, respectively, and the first regulating valves, second regulating valves, and third regulating valves are connected to the PLC system for electrical signal connection.

[0015] Optionally, in step 1, the liquid amylase activity is 600-1200 U / ml, the cellulase activity is 600-1200 U / ml, the lipase activity is 1800-3000 U / ml, and the glucose oxidase activity is 600 U / ml. The four are mixed in proportion to prepare a 20 kg mixture, and 2 kg of glucose is added.

[0016] Optionally, in steps 1-3, spray and wash for 10 minutes each, then pause, soak and react for 15 minutes, and in step 4, wash for 5 minutes.

[0017] Furthermore, to achieve the above objectives, the present invention also provides a biological enzyme preparation cleaning system, the system comprising a granulation dryer and a biological enzyme preparation tank connected to the granulation dryer. The granulation dryer includes a collection chamber, a granulation chamber, and a base, arranged from top to bottom. The collection chamber, granulation chamber, and base are respectively connected to the biological enzyme preparation tank via a main pipeline. A booster pump is installed in the main pipeline. The biological enzyme preparation tank includes a compound enzyme premix tank, a catalase tank, an alkaline protease tank, and a reverse osmosis water tank. The compound enzyme premix tank, catalase tank, alkaline protease tank, and reverse osmosis water tank are respectively connected to the main pipeline via corresponding first, second, third, and fourth switching valves.

[0018] Optionally, the booster pump, the first switch valve, the second switch valve, the third switch valve, and the fourth switch valve are respectively connected to the PLC system electrical signals.

[0019] Beneficial effects:

[0020] The biological enzyme cleaning method of this invention uses liquid amylase, cellulase, lipase, glucose oxidase, and glucose to spray and wash the collection chamber, feeding chamber, and base of the granulation dryer. It then uses catalase for separate spraying, and finally alkaline protease for separate spraying. This step-by-step cleaning in a specific order and timing avoids the alkaline protease from decomposing other enzymes, thus achieving thorough decomposition of sugars, oils, and proteins. Furthermore, during the first enzymatic wash, the all-around spraying of glucose oxidase and glucose, under aerobic conditions, causes the glucose and glucose oxidase to react and produce a low concentration of hydrogen peroxide. This in-situ hydrogen peroxide generation provides timely and comprehensive disinfection and sterilization of the granulation dryer's interior. Simultaneously, the addition of polysaccharide hydrolytic enzymes such as amylase and cellulase in the first enzymatic wash allows the small-molecule sugars generated from the hydrolysis of sugary contaminants by these enzymes to serve as substrates for subsequent glucose oxidase action. Increasing the substrate concentration promotes hydrogen peroxide generation and enhances the disinfection effect. After sterilization, catalase is sprayed to decompose residual hydrogen peroxide, preventing it from entering the product. Finally, reverse osmosis water is sprayed to thoroughly clean the inside of the equipment, achieving a complete cleaning effect. The entire cleaning process is precisely controlled, using minimal water and electricity, and taking only a short time, making it environmentally friendly.

[0021] The system also includes a biological enzyme preparation cleaning system. This system connects the granulation dryer to the biological enzyme preparation tank. The granulation dryer comprises a collection chamber, a granulation chamber, and a base (from top to bottom). The collection chamber, granulation chamber, and base are connected to the biological enzyme preparation tank via main pipelines. A booster pump is installed in the main pipelines. The biological enzyme preparation tank includes a compound enzyme premix tank, a catalase tank, an alkaline protease tank, and a reverse osmosis water tank. This system allows the compound enzyme premix tank, catalase tank, alkaline protease tank, and reverse osmosis water tank to clean the collection chamber, granulation chamber, and base respectively, preventing the protease from decomposing other enzymes. This method is cost-effective. Furthermore, each tank and nozzle is equipped with a pneumatic diaphragm valve and connected to the granulation dryer's PLC system. This allows for individual setting of the spraying time for each tank and automatic cleaning as required, achieving one-button cleaning and intelligent cleaning. Attached Figure Description

[0022] Figure 1 This is a flowchart of an embodiment of a biological enzyme cleaning method according to the present invention;

[0023] Figure 2 This is a schematic diagram of an embodiment of a biological enzyme preparation cleaning system according to the present invention.

[0024] Explanation of icon numbers

[0025] 1. Compound enzyme premixing tank; 2. Catalase tank; 3. Alkaline protease tank; 4. Reverse osmosis water tank; 5. Main pipeline; 6. First branch pipeline; 7. Second branch pipeline; 8. Third branch pipeline; 101. First switch valve; 201. Second switch valve; 301. Third switch valve; 401. Fourth switch valve; 501. Variable frequency booster pump; 601. First regulating valve; 701. Second regulating valve; 801. Third regulating valve; a. Base; c. Collection chamber; b. Granulation chamber

[0026] The realization of the objective, functional features and advantages of the present invention will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0027] It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

[0028] See Figure 1-2 This invention provides a method for cleaning biological enzyme preparations, which is applied to a biological enzyme preparation cleaning system, such as... Figure 2 As shown, the system includes a granulation dryer and a bio-enzyme preparation tank connected to the granulation dryer. The granulation dryer is connected to the bio-enzyme preparation tank via a main pipeline 5. The dryer body includes a collection chamber c, a granulation chamber b, and a base a, distributed from top to bottom. The collection chamber c, granulation chamber b, and base a are respectively connected to the main pipeline 5 via corresponding branch pipelines. A variable frequency booster pump 501 is installed in the main pipeline 5. The bio-enzyme preparation tank includes a compound enzyme premix tank 1, a catalase tank 2, an alkaline protease tank 3, and a reverse osmosis water tank 4. The compound enzyme premix tank 1, catalase tank 2, alkaline protease tank 3, and reverse osmosis water tank 4 are respectively connected to the main pipeline 501. The compound enzyme premix tank 1 contains liquid amylase, cellulase, lipase, and glucose oxidase.

[0029] Furthermore, the compound enzyme premixing tank 1, catalase tank 2, alkaline protease tank 3, and reverse osmosis water tank 4 are respectively connected to the main pipeline 501 via corresponding first switch valve 101, second switch valve 201, third switch valve 301, and fourth switch valve 401. Preferably, the variable frequency booster pump 501, the first switch valve 101, the second switch valve 201, the third switch valve 301, and the fourth switch valve 401 are respectively connected to the PLC system via electrical signals, thereby realizing intelligent control of each tank through the PLC system. Preferably, the PLC system is equipped with a timing module and a sequence control module, which are respectively connected to the electrical signals of each switch valve to trigger the opening and closing operations of each tank sequentially according to preset time nodes.

[0030] Furthermore, the compound enzyme premixing tank 1, catalase tank 2, alkaline protease tank 3, and reverse osmosis water tank 4 are also equipped with corresponding liquid level sensors. Each liquid level sensor is connected to the PLC system to provide real-time feedback on the liquid level inside each tank, so as to further adjust the opening of the corresponding switch valve.

[0031] Furthermore, the PLC system also includes a feedback control module. This module receives real-time signals from the level and pressure sensors of each receiving tank, dynamically adjusting the speed of the variable frequency booster pump 501 and the opening of the corresponding regulating valve. For example, if nozzle blockage causes a sudden pressure surge, and the pressure sensor 51 detects that the pressure exceeds the maximum value, the PLC triggers an emergency response, controlling the variable frequency booster pump 501 to immediately reduce its frequency to the minimum speed and simultaneously controlling the corresponding regulating valve to open to 100%, thus reducing the pressure in the main pipeline through diversion. If the pressure still exceeds the limit, the PLC closes the switching valve and triggers an alarm. Similarly, if the tank level is too low, and the corresponding level sensor detects that the level is below 20%, the PLC executes protection logic, controlling the variable frequency booster pump 501 to reduce its frequency to the minimum speed required to maintain pressure and closing the corresponding switching valve of the tank. Through PLC timing control and pressure feedback regulation, manual intervention is reduced, and operational errors are minimized. Furthermore, variable frequency speed control and valve fine-tuning reduce energy consumption by 30% and simultaneously reduce mechanical wear on the equipment caused by high-pressure flushing.

[0032] Furthermore, a first branch pipe 6, a second branch pipe 7, and a third branch pipe 8 are respectively arranged between the collection chamber, the granulation chamber, and the base and the main pipeline. Rotary nozzles are respectively installed at the ends of the first branch pipe 6, the second branch pipe 7, and the third branch pipe 8. Preferably, each rotary nozzle is a multi-degree-of-freedom oscillating nozzle with a coverage angle of 120-360° and a nozzle diameter of 0.5-1.2mm. More preferably, each rotary nozzle is also electrically linked to a PLC system; that is, the PLC system indirectly controls the start / stop, speed, or angle of the rotary nozzle by driving the motor and angle adjustment structure of the rotary nozzle through an output module. Additionally, each rotary nozzle is externally fitted with a corrosion-resistant, anti-static, and heat-dissipating outer shell.

[0033] Furthermore, the first branch pipe 6, the second branch pipe 7, and the third branch pipe 8 are each equipped with a corresponding first regulating valve 601, a second regulating valve 701, and a third regulating valve 801. These valves are electrically operated, and their opening and closing states are independently controlled by the PLC system via a set of solenoid valves according to a preset sequence. This allows for individual setting of the spraying time for the solution in each tank, enabling automatic cleaning as required, achieving one-button cleaning. Specifically, when the granulation dryer needs cleaning, the user enters the PLC control panel, clicks the one-button cleaning button, and various materials, valves, nozzles, booster pumps, etc., work together according to set parameters to clean the interior of the equipment.

[0034] Furthermore, the opening degree of the electric regulating valve is dynamically adjusted by the PLC based on the pressure (P) and flow rate (Q) of the main pipeline 5, which satisfies:

[0035] (k is the pipeline characteristic coefficient).

[0036] Furthermore, the first switch valve 101, the second switch valve 201, the third switch valve 301, and the fourth switch valve 401 are all pneumatic diaphragm valves, and their opening and closing states are controlled by the PLC system.

[0037] Furthermore, the method includes the following steps:

[0038] Step 1: Glucose is added to the compound enzyme premix tank 1 to prepare a mixed solution. This solution is then sprayed into various areas inside the granulator dryer via the main pipeline 5 for the first cleaning. Glucose and glucose oxidase react in situ on the surface of the dirt on the inner wall of the granulator dryer to produce hydrogen peroxide, which disinfects and sterilizes the interior. The concentration of hydrogen peroxide produced in this step is 0.2-0.6%. This step uses a bio-enzymatic method to prepare hydrogen peroxide in situ, ensuring stability at low concentrations and guaranteeing its disinfection and sterilization effect. This solves the problems caused by excessively high concentrations of traditional hydrogen peroxide disinfectant solutions. The stability of hydrogen peroxide is affected by several factors: ① Temperature: Temperature is a crucial factor affecting hydrogen peroxide decomposition. The decomposition rate increases with temperature. At room temperature, the decomposition rate is slow, but at high temperatures, the decomposition reaction proceeds rapidly. ② Light: Light also promotes the decomposition of hydrogen peroxide. Exposure to strong light, such as ultraviolet light, can break the chemical bonds in hydrogen peroxide molecules, thus initiating a decomposition reaction. ③ Catalysts: Certain substances can accelerate the decomposition of hydrogen peroxide. These substances are called catalysts, such as metal ions and enzymes. Under the action of a catalyst, the decomposition rate of hydrogen peroxide will be significantly increased. ④ Pressure: Pressure also has a certain impact on the decomposition of hydrogen peroxide. Under high pressure, the decomposition rate of hydrogen peroxide may decrease. Conversely, under reduced pressure or vacuum conditions, the decomposition of hydrogen peroxide may be accelerated. ⑤ Impurities: Impurities in hydrogen peroxide may also trigger its decomposition. Some impurities can act as catalysts, promoting the decomposition of hydrogen peroxide. Therefore, these factors need to be carefully controlled during storage and use. Furthermore, the low-concentration hydrogen peroxide obtained in the above steps, after sterilization, can also reduce the amount of catalase used subsequently, reducing cleaning costs. Therefore, the bio-enzymatic method for preparing hydrogen peroxide has the advantages of high efficiency, specificity, and safety.

[0039] Preferably, the activity ratio of liquid amylase, cellulase, lipase and glucose oxidase in the compound enzyme premix tank 1 is (1-2):(1-2):(3-5):1, and the ratio of the total mass of the mixed solution of amylase, cellulase, lipase and glucose oxidase to the mass of glucose is (8-10):1.

[0040] Step 2: The catalase solution in the catalase tank 2 is sprayed into various areas inside the granulator dryer through the main pipeline 501 to perform a second spraying of the granulator dryer so that the catalase can effectively decompose hydrogen peroxide. The activity ratio of the catalase solution in the catalase tank 2 to glucose oxidase is (20-40):3.

[0041] Step 3: The alkaline protease solution in the alkaline protease tank 3 is sprayed into various areas inside the granulator dryer through the main pipeline 501 to perform the third spray washing of the granulator dryer. The activity ratio of the alkaline protease solution to the catalase solution is 3:(2-4).

[0042] Step 4: Reverse osmosis water from the reverse osmosis water tank 4 is sprayed into various areas inside the granulator dryer through the main pipeline 501 to perform the fourth spray washing of the granulator dryer.

[0043] Further, in step 1, the liquid amylase has an activity of 1200 U / ml, the cellulase has an activity of 1200 U / ml, the lipase has an activity of 3000 U / ml, and the glucose oxidase has an activity of 600 U / ml. These four are mixed in a specific ratio to prepare a 20 kg solution, and 2 kg of glucose is added. In step 2, the catalase solution has an activity of 8000 U / ml and a mass of 12 kg, and in step 3, the alkaline protease solution has an activity of 6000 U / ml and a mass of 15 kg.

[0044] Furthermore, in actual use, the spraying process is controlled to last 10 minutes in step 1, and 5 minutes in steps 2-4 respectively, with a 15-minute pause between adjacent steps for soaking reaction. The entire cleaning process is precisely controlled, using less water and electricity, taking less time, and is environmentally friendly.

[0045] Granulation dryers are specialized stainless steel equipment used continuously in the production of industrial enzyme preparations. Due to the numerous internal structural components, cleaning is time-consuming, labor-intensive, and often incomplete. The inner walls are heavily fouled, with an average thickness of 0.2-1 mm. The fouling layer is light yellow and primarily consists of dextrin, starch, cellulose, fats, proteins, and other biomolecules commonly used as excipients in biological enzymes. To address this heavily fouled inner wall, the following examples and comparative embodiments employ a one-button enzymatic automatic descaling and cleaning method.

[0046] Example 1

[0047] Step 1, Ingredient Preparation: First, mix 1200U / ml liquid amylase, 1200U / ml cellulase, 3000U / ml liquid lipase, and 600U / ml glucose oxidase in an activity ratio of 2:2:5:1 to prepare 20kg of mixed product. Clean the No. 1 material cart of the compound enzyme premix tank and add it. Set aside. Add 2kg of glucose before starting the spraying process and dissolve it. Prepare 12kg of liquid catalase with an activity of 8000U / ml. Clean the No. 2 material cart of catalase and add it. Set aside. Prepare 15kg of liquid alkaline protease with an activity of 6000U / ml. Clean the No. 3 material cart of alkaline protease and add it. Set aside. Clean the No. 4 material cart of reverse osmosis water and add 180kg of reverse osmosis water. Set aside.

[0048] Step 2, Parameter Setting: On the PLC control panel of the granulation dryer, set a time of 10 minutes to spray the material of car #1, a pause time of 15 minutes, a time of 5 minutes to spray the material of car #2, a time of 5 minutes to spray the material of car #3, a pause time of 15 minutes, and a time of 5 minutes to spray the material of car #4. All the above times should be set once before cleaning.

[0049] Step 3: When cleaning the granulator dryer is required, access the PLC control panel and click the one-button cleaning button. Various materials, valves, nozzles, booster pumps, etc., will be sprayed in sequence according to the set time: 10 minutes to finish spraying material from cart #1, 15 minutes pause, 5 minutes to finish spraying material from cart #2, 5 minutes to finish spraying material from cart #3, 15 minutes pause, and 5 minutes to finish spraying material from cart #4. This coordinated process cleans the interior of the equipment. The cleaned granulator dryer's internal dirt removal and microbial data will then be monitored.

[0050] Example 2

[0051] Step 1, Ingredient Preparation: First, mix 1200U / ml liquid amylase, 1200U / ml cellulase, 3000U / ml liquid lipase, and 600U / ml glucose oxidase in an activity ratio of 2:2:3:1 to prepare 20kg of mixed product. Clean the No. 1 material cart of the compound enzyme premix tank and add it. Set aside. Add 2kg of glucose before starting the spraying process and dissolve it. Prepare 12kg of liquid catalase with an activity of 8000U / ml. Clean the No. 2 material cart of catalase and add it. Set aside. Prepare 15kg of liquid alkaline protease with an activity of 6000U / ml. Clean the No. 3 material cart of alkaline protease and add it. Set aside. Clean the No. 4 material cart of reverse osmosis water and add 180kg of reverse osmosis water. Set aside.

[0052] Step 2, Parameter Setting: On the PLC control panel of the granulation dryer, set a time of 10 minutes to spray the material of car #1, a pause time of 15 minutes, a time of 5 minutes to spray the material of car #2, a time of 5 minutes to spray the material of car #3, a pause time of 15 minutes, and a time of 5 minutes to spray the material of car #4. All the above times should be set once before cleaning.

[0053] Step 3: When cleaning the granulator dryer is required, access the PLC control panel and click the one-button cleaning button. Various materials, valves, nozzles, booster pumps, etc., will be sprayed in sequence according to the set time: 10 minutes to finish spraying material from cart #1, 15 minutes pause, 5 minutes to finish spraying material from cart #2, 5 minutes to finish spraying material from cart #3, 15 minutes pause, and 5 minutes to finish spraying material from cart #4. This coordinated process cleans the interior of the equipment. The cleaned granulator dryer's internal dirt removal and microbial data will then be monitored.

[0054] Example 3

[0055] Step 1, Ingredient Preparation: First, mix 1200U / ml liquid amylase, 1200U / ml cellulase, 3000U / ml liquid lipase, and 600U / ml glucose oxidase in an activity ratio of 1:2:3:1 to prepare 20kg of mixed product. Clean the No. 1 material cart of the compound enzyme premix tank and add it. Set aside. Add 2kg of glucose before starting the spraying process and dissolve it. Prepare 12kg of liquid catalase with an activity of 8000U / ml. Clean the No. 2 material cart of catalase and add it. Set aside. Prepare 15kg of liquid alkaline protease with an activity of 6000U / ml. Clean the No. 3 material cart of alkaline protease and add it. Set aside. Clean the No. 4 material cart of reverse osmosis water and add 180kg of reverse osmosis water. Set aside.

[0056] Step 2, Parameter Setting: On the PLC control panel of the granulation dryer, set a time of 10 minutes to spray the material of car #1, a pause time of 15 minutes, a time of 5 minutes to spray the material of car #2, a time of 5 minutes to spray the material of car #3, a pause time of 15 minutes, and a time of 5 minutes to spray the material of car #4. All the above times should be set once before cleaning.

[0057] Step 3: When cleaning the granulator dryer is required, access the PLC control panel and click the one-button cleaning button. Various materials, valves, nozzles, booster pumps, etc., will be sprayed in sequence according to the set time: 10 minutes to finish spraying material from cart #1, 15 minutes pause, 5 minutes to finish spraying material from cart #2, 5 minutes to finish spraying material from cart #3, 15 minutes pause, and 5 minutes to finish spraying material from cart #4. This coordinated process cleans the interior of the equipment. The cleaned granulator dryer's internal dirt removal and microbial data will then be monitored.

[0058] Furthermore, to better demonstrate the cleaning effect of the present invention, the following comparative experiment was conducted. Specifically:

[0059] Comparative Example 1

[0060] Similar to steps 1 and 2 of Example 1, in step 3, the material from carts 1-3 is simultaneously fed into the granulation dryer via the PLC control panel. The spraying time is controlled to be 10 minutes, followed by a 15-minute pause, and finally, the material from cart 4 is sprayed for the last 5 minutes. The removal of dirt and microbial data inside the granulation dryer after cleaning are then monitored.

[0061] Comparative Example 2

[0062] The process is essentially the same as steps 2 and 3 of Example 1, except that glucose oxidase is not added to the No. 1 material car of the compound enzyme premixing tank in step 1. After cleaning, the removal of dirt and microbial data inside the granulation dryer are checked.

[0063] Comparative Example 3

[0064] The process is essentially the same as steps 2 and 3 of Example 1, except that in step 1, the amount of amylase used in the No. 1 material cart of the compound enzyme premixing tank is reduced. 600 U / ml liquid amylase, 1200 U / ml cellulase, 3000 U / ml liquid lipase, and 1200 U / ml glucose oxidase are mixed and prepared at an activity ratio of 0.5:2:5:1. After cleaning, the removal of dirt and microbial data inside the granulation dryer are tested.

[0065] Comparative Example 4

[0066] The process is essentially the same as steps 2 and 3 of Example 1, except that in step 1, the amount of cellulase used in the No. 1 material cart of the compound enzyme premixing tank is reduced. 1200 U / ml liquid amylase, 600 U / ml cellulase, 3000 U / ml liquid lipase, and 1200 U / ml glucose oxidase are mixed and prepared at an activity ratio of 2:0.5:5:1. After cleaning, the removal of dirt and microbial data inside the granulation dryer are tested.

[0067] Comparative Example 5

[0068] The process is essentially the same as steps 2 and 3 of Example 1, except that in step 1, the amount of lipase used in the No. 1 material cart of the compound enzyme premixing tank is reduced. 1200 U / ml liquid amylase, 1200 U / ml cellulase, 1800 U / ml liquid lipase, and 1200 U / ml glucose oxidase are mixed and prepared in an activity ratio of 2:2:3:1. After cleaning, the removal of dirt and microbial data inside the granulation dryer are tested.

[0069] Comparative Example 6

[0070] The process is essentially the same as steps 2 and 3 of Example 1, except that in step 1, a mixture of liquid amylase, cellulase, lipase, and 3% hydrogen peroxide solution is directly added to the No. 1 material cart of the compound enzyme premix tank. After cleaning, the removal of dirt and microbial data inside the granulation dryer are tested.

[0071] Comparative Example 7

[0072] The process is essentially the same as steps 2 and 3 in Example 1, except that catalase is not added to the catalase tank #2 in step 1. After cleaning, the removal of dirt and microbial data inside the granulator dryer are checked.

[0073] Comparative Example 8

[0074] The process is essentially the same as steps 2 and 3 in Example 1, except that in step 1, alkaline protease is not added to the alkaline protease tank #3. After cleaning, the removal of dirt and microbial data inside the granulator dryer are checked.

[0075] For each embodiment and comparative example, the waste liquid from the final reverse osmosis water cleaning step was collected. Referring to the standard "Test Methods for Lactic Acid Bacteria in Imported and Exported Foods" (SN / T 1941.1-2017), viable bacteria in the cleaning waste liquid were isolated and counted using a gradient dilution method. The removal of dirt from the inner wall of the granulator was evaluated by observation. The results of the cleaning and disinfection / sterilization effects of each embodiment and comparative example are shown in Table 1.

[0076] Table 1

[0077]

[0078]

[0079] As described above, Examples 1-3 employ stepwise cleaning, while in Comparative Example 1, all enzymes were simultaneously added to the granulator dryer. The final cleaning result showed no significant change in the scale layer, with dirt adhering to the inner wall, and an average microbial count of 11899 CFU / ml. This demonstrates that the composite enzyme stepwise cleaning method of the present invention avoids the degradation of other enzymes by proteases, effectively removing dirt from the stainless steel granulator. Furthermore, Comparative Example 2 did not add glucose oxidase, thus failing to achieve the reaction between glucose oxidase and glucose to generate hydrogen peroxide. Although the scale layer disappeared after cleaning, the inner wall retained the original metallic color of stainless steel. However, a certain amount of microorganisms still remain inside. This indicates that the glucose oxidase of the present invention reacts with glucose, effectively improving the in-situ disinfection and sterilization effect of low-concentration hydrogen peroxide. Meanwhile, in Comparative Examples 3-5, the dosage of amylase, cellulase, and lipase was reduced accordingly, resulting in sticky sugary or greasy contaminants on the inner wall, which easily breeds a certain amount of microorganisms. In Comparative Example 6, a mixture of 3% hydrogen peroxide solution was directly used for cleaning; after cleaning with high-concentration hydrogen peroxide alone, the scale layer showed no significant change, and contaminants adhered to the inner wall, also containing a certain amount of microorganisms. In Comparative Example 7, no catalase was added; some residual hydrogen peroxide in the tank denatured the subsequently added protease, reducing enzyme activity, leading to incomplete degradation of protein contaminants and enzymes introduced in the previous cleaning steps, resulting in a certain amount of microorganisms remaining inside. In Comparative Example 8, no alkaline protease was added; protein contaminants could not be thoroughly cleaned during the cleaning process, resulting in contaminants adhering to the inner wall, ultimately also containing a certain amount of microorganisms.

[0080] It should be noted that, in this document, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or system that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or system. Unless otherwise specified, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or system that includes that element.

[0081] The sequence numbers of the above embodiments of the present invention are for descriptive purposes only and do not represent the superiority or inferiority of the embodiments.

[0082] The above are merely preferred embodiments of the present invention and do not limit the scope of the patent. Any equivalent structural or procedural transformations made based on the description and drawings of the present invention, or direct or indirect applications in other related technical fields, are similarly included within the scope of patent protection of the present invention.

Claims

1. A method for cleaning with a biological enzyme preparation, characterized in that, The method is applied to a biological enzyme preparation cleaning system. The system includes a granulation dryer and a biological enzyme preparation tank connected to the granulation dryer. The granulation dryer is connected to the biological enzyme preparation tank via a main pipeline. The biological enzyme preparation tank includes a compound enzyme premix tank, a catalase tank, an alkaline protease tank, and a reverse osmosis water tank. The compound enzyme premix tank, catalase tank, alkaline protease tank, and reverse osmosis water tank are each connected to the main pipeline. The method includes the following steps: Step 1: Add glucose to the compound enzyme premix tank and prepare a mixed solution. Spray the mixed solution into each area inside the granulator dryer through the main pipeline for the first spray cleaning of the granulator dryer. The compound enzyme premix tank is filled with liquid amylase, cellulase, lipase and glucose oxidase with an activity ratio of (1-2):(1-2):(3-5):1, so that the glucose and glucose oxidase inside the granulator dryer react to produce hydrogen peroxide, and the hydrogen peroxide disinfects and sterilizes the inside of the granulator dryer. The ratio of the total mass of the mixed solution of amylase, cellulase, lipase and glucose oxidase to the mass of glucose is (8-10):

1. Step 2: The catalase solution in the catalase tank is sprayed into various areas inside the granulator dryer through the main pipeline to perform a second spraying of the granulator dryer so that the catalase can effectively decompose hydrogen peroxide. The ratio of the activity of the catalase solution in the catalase tank to that of glucose oxidase is (20-40):

3. Step 3: The alkaline protease solution in the alkaline protease tank is sprayed into various areas inside the granulator dryer through the main pipeline for the third spray washing of the granulator dryer. The activity ratio of the alkaline protease solution to the catalase solution is 3:(2-4). Step 4: Reverse osmosis water from the reverse osmosis water tank is sprayed into various areas inside the granulator dryer through the main pipeline to perform the fourth spray washing of the granulator dryer.

2. The biological enzyme preparation cleaning method as described in claim 1, characterized in that, The granulation dryer includes, from top to bottom, a collection chamber, a granulation chamber, and a base. The collection chamber, granulation chamber, and base are respectively connected to the biological enzyme preparation tank through a main pipeline.

3. The biological enzyme preparation cleaning method as described in claim 2, characterized in that, The collection chamber, granulation chamber, and base are respectively connected to the main pipeline by a first branch pipe, a second branch pipe, and a third branch pipe, and a rotating nozzle is installed at the end of each of the first branch pipe, the second branch pipe, and the third branch pipe.

4. The biological enzyme preparation cleaning method as described in claim 1, characterized in that, The premixed compound enzyme tank, catalase tank, alkaline protease tank, and reverse osmosis water tank are equipped with corresponding first, second, third, and fourth switching valves between themselves and the main pipeline. Each of the first, second, third, and fourth switching valves is connected to an external PLC system for electrical signal transmission.

5. The biological enzyme preparation cleaning method as described in claim 1, characterized in that, A booster pump is also installed in the main pipeline, and the booster pump is electrically connected to an external PLC system.

6. The biological enzyme preparation cleaning method as described in claim 3, characterized in that, The first branch pipe, the second branch pipe, and the third branch pipe are each equipped with a corresponding first regulating valve, a second regulating valve, and a third regulating valve, which are connected to the external PLC system for electrical signals.

7. The biological enzyme preparation cleaning method according to any one of claims 1 to 6, characterized in that, In step 1, the liquid amylase activity is 600-1200 U / ml, the cellulase activity is 600-1200 U / ml, the lipase activity is 1800-3000 U / ml, and the glucose oxidase activity is 600 U / ml. The four are mixed in proportion to prepare a 20 kg mixture, and 2 kg of glucose is added.

8. The biological enzyme preparation cleaning method as described in claim 7, characterized in that, Spray wash for 10 minutes in step 1, and spray wash for 5 minutes in steps 2, 3 and 4 respectively, with a 15-minute pause between each adjacent step to allow for soaking reaction.

9. A biological enzyme preparation cleaning system for use with the method according to any one of claims 1 to 8, characterized in that, The system includes a granulation dryer and a biological enzyme preparation tank connected to the granulation dryer. The granulation dryer includes a collection chamber, a granulation chamber, and a base distributed from top to bottom. The collection chamber, granulation chamber, and base are respectively connected to the biological enzyme preparation tank through a main pipeline. A booster pump is installed in the main pipeline. The biological enzyme preparation tank includes a compound enzyme premix tank, a catalase tank, an alkaline protease tank, and a reverse osmosis water tank. The compound enzyme premix tank, catalase tank, alkaline protease tank, and reverse osmosis water tank are respectively connected to the main pipeline through corresponding first, second, third, and fourth switching valves.

10. The biological enzyme preparation cleaning system according to claim 9, characterized in that, The booster pump, the first switch valve, the second switch valve, the third switch valve, and the fourth switch valve are respectively connected to the PLC system electrical signals.

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