Recombinant vector of high-purity active peptide, recombinant cell and production and purification method

Abstract

The invention provides a recombinant vector, a recombinant cell and a production and purification method of a high-purity active peptide, and belongs to the technical field of genetic engineering. The invention provides a recombinant vector for expressing an active peptide, a coding gene of the active peptide to be expressed is connected with a cleavable self-aggregation tag to form a fusion protein gene, and the fusion protein gene is connected with a basic vector to obtain the recombinant vector. A genetic transformation method is utilized, the recombinant vector is transferred into a host cell to be cultured, an aggregate of the fusion protein can be obtained after cell lysis, the aggregate of the fusion protein is cut, and an obtained product is a crude product of the active peptide. According to the invention, the high-purity active peptide can be obtained only through one-step standardized ultrafiltration of the crude product, and the purity can exceed 99% through verification. The method is simple, convenient and rapid, the process is greatly simplified, the period is greatly shortened, scale amplification is facilitated, and commercialized production is facilitated.

Images