一种免加热快速检测枝葡霉的引物、试剂盒、检测方法及其应用
By using specific primers and the ELbCas12a enzyme combined with the RAA reaction system, the problem of rapid detection of Staphylococcus aureus without the need for heating instruments has been solved, achieving highly sensitive and specific detection of edible fungal pathogens, suitable for field applications.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- JILIN AGRICULTURAL UNIV
- Filing Date
- 2025-06-11
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies are insufficient for the rapid, sensitive, and heat-free detection of Staphylococcus aureus pathogens in edible fungi. Traditional methods are time-consuming and require specific testing instruments, which limits the effectiveness of early monitoring and control.
A rapid detection method without heating was developed using specific primers, ELbCas12a enzyme, and crRNA in combination with a RAA reaction system. The pathogen was detected by performing RAA amplification and CRISPR/Cas12a reaction at room temperature, utilizing the trans-cleavage activity of ELbCas12a.
It achieves high sensitivity, strong specificity and low background interference detection of Staphylococcus aureus at room temperature, and can obtain test results within 1 hour, making it suitable for on-site testing with limited resources.
Smart Images
Figure CN120683289B_ABST