A method for artificial breeding of oysters

Abstract

The present application relates to oyster artificial breeding technical field, especially to a kind of method for artificial breeding oyster.It includes the following steps: (1) selecting oyster parent, after maturation, artificial insemination, hatching, obtain oyster larvae;The maturation uses maturation agent;(2) the oyster larvae obtained in step (1) is cultivated, and feeding opening bait is fed, and oyster seedling is obtained, and after oyster seedling attachment metamorphosis, oyster artificial feed is fed;(3) the oyster seedling obtained in step (2) is cultivated, and juvenile is obtained;(4) the juvenile is moved to the breeding farm and is bred for 12-18 months.The present application realizes the high yield, high efficiency and high quality of oyster culture by innovative maturation technology, precise nutrition allocation and scientific environmental management, provides reliable solution for the sustainable development of oyster industry, and has broad application prospect.

Description

Technical Field

[0001] This invention relates to the field of oyster culture technology, and more particularly to a method for artificially culturing oysters. Background Technology

[0002] Oysters are one of the most common shellfish along the coast, commonly known as oysters, oyster meat, or sea oysters. Their soft body contains 45%–57% protein, 7%–11% fat, and 19%–38% glycogen, as well as various vitamins and ash, making them highly nutritious and earning them the nickname "sea milk." Besides their delicious flavor, oyster meat also has certain medicinal value. Oysters are low in cholesterol and extremely high in zinc, which helps improve male sexual function. Furthermore, oyster shells are a good raw material for making lime, and can also be used to process buttons and handicrafts, or ground into powder as a feed additive for poultry and livestock. Therefore, oysters are in short supply and expensive in some large and medium-sized cities, while current oyster farming technology is not yet perfect, and oyster production cannot fully meet market demand.

[0003] For example, natural seed collection is greatly affected by the environment, resulting in inconsistent seed quality; artificial seed cultivation is costly and requires strict technical expertise. High-density artificial farming can easily lead to slow growth and increased disease, while low-density farming affects economic efficiency. Furthermore, different oyster varieties have different requirements for environmental conditions (such as water temperature and salinity), making existing farming methods difficult to apply universally.

[0004] Therefore, how to provide a method for artificially cultivating oysters to solve the above-mentioned technical problems is a problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0005] The purpose of this invention is to provide a method for artificially cultivating oysters.

[0006] To achieve the above-mentioned objectives, the present invention provides the following technical solution:

[0007] This invention provides a method for artificially cultivating oysters, comprising the following steps:

[0008] (1) Select oyster parents, ripen them, then artificially inseminate and hatch them to obtain oyster larvae; ripening agents are used during ripening; the ripening agents are: L-acetylcarnitine, marine red yeast extract, brown algae extract, Rehmannia glutinosa extract and placenta extract;

[0009] (2) Cultivate the oyster larvae obtained in step (1), feed them with starter feed, and obtain oyster seedlings. After the oyster seedlings undergo metamorphosis, feed them with artificial oyster feed.

[0010] (3) The oyster seedlings obtained in step (2) are cultured in an intermediate stage to obtain juvenile oysters;

[0011] (4) The juvenile clams are transferred to a breeding farm and raised for 12 to 18 months.

[0012] Preferably, in step (1), the oyster parent stock is temporarily kept in an outdoor holding tank for 20-30 days, and then moved to an indoor ripening tank for ripening treatment.

[0013] Preferably, artificial oyster feed is used during both the temporary holding and ripening stages; both the temporary holding pond and the ripening pond use naturally circulating seawater as the aquaculture water.

[0014] Preferably, the initial feed consists of: *Isochrysis var. *zhanjiangensis*, *Chaetoceros*, *Platycodon*, and *Phaeodactylum tricornutum*; the mass ratio of *Isochrysis var. *zhanjiangensis*, *Chaetoceros*, *Platycodon*, and *Phaeodactylum tricornutum* is 1–3:3–1:3–1:3–1.

[0015] Preferably, during the larval rearing process described in step (2), before the eye spots appear, 3 / 5 of the water is changed daily at a temperature of 21-22°C. After the eye spots appear, the water is changed twice daily, with 1 / 2 of the water changed each time at a temperature of 24-26°C.

[0016] Preferably, in step (2), the proportion of artificial oyster feed fed is 4-6% of the larvae's body weight; in step (3), artificial oyster feed is fed during intermediate rearing at a proportion of 5-7% of the larvae's body weight.

[0017] Preferably, the intermediate cultivation time in step (3) is 65 to 85 days.

[0018] Preferably, the mass ratio of L-acetylcarnitine, marine red yeast extract, brown algae extract, prepared rehmannia root extract, and placenta extract in step (1) is 4-6:9-13:20-30:10-16:10-16.

[0019] Preferably, the oyster artificial feed is prepared from the following components in parts by weight: 25-35 parts wheat flour, 8-12 parts puffed soybean flour, 6-10 parts corn flour, 20-40 parts protein raw material, 20-40 parts algae raw material, 3-5 parts calcium dihydrogen phosphate, 2-4 parts zeolite powder, 1-2 parts choline chloride, 2-3 parts compound vitamins, and 0.8-1.2 parts fermentation agent; the protein raw material is: black soldier fly powder, earthworm powder, fly larvae powder, and silkworm pupa powder; the algae raw material is: Chaetoceros, Cyclosporium, Seaweed, and Chlorella; the fermentation agent is: Bacillus coagulans, Bacillus licheniformis, Aspergillus niger, Candida albicans, Pediococcus pentosaceus, and Lactobacillus rhamnosus.

[0020] Preferably, the compound vitamins are vitamin D, vitamin E, vitamin K, vitamin B2 and vitamin B12; the mass ratio of vitamin D, vitamin E, vitamin K, vitamin B2 and vitamin B12 is 12-16:8-12:12-16:5-9:6-10;

[0021] The mass ratio of Bacillus coagulans, Bacillus licheniformis, Aspergillus niger, Candida albicans, Pediococcus pentosus, and Lactobacillus rhamnosus is 16–20:15–17:10–14:7–11:6–10:6–10;

[0022] The mass ratio of black soldier fly powder, earthworm powder, fly larvae powder, and silkworm pupa powder is 20-30:10-20:5-9:3-7; the mass ratio of Chaetoceros, Cyclostomata, Seaweed, and Chlorella is 8-12:8-12:8-12:8-12.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] 1. Improved reproductive efficiency: This invention significantly improves the ripening effect of oyster parent oysters by using a specially formulated ripening agent, resulting in increased egg diameter, significantly improved fertilization rate and hatching rate, and solving the problem of unstable quality of naturally collected oysters.

[0025] 2. Optimized growth performance: This invention uses a scientifically formulated starter feed and artificial feed to promote the rapid growth of oyster larvae and seedlings. The experimental group reached a shell length of 13.4 mm at 60 days of age, significantly better than the control group, and the survival rate increased to 73.8%.

[0026] 3. Wide environmental adaptability: This invention adapts to the needs of oysters at different growth stages by adjusting parameters such as water temperature, water exchange frequency and salinity in stages, reducing the impact of environmental fluctuations on oysters. At the same time, it regulates and promotes oyster growth through the external environment, making it suitable for a variety of aquaculture scenarios.

[0027] 4. Standardized operation: This invention adopts quantitative management throughout the entire process from parent stock rearing and ripening to intermediate cultivation. The method is simple, suitable for large-scale promotion, and effectively solves the problem of the difficulty in standardizing traditional breeding methods.

[0028] In summary, this invention achieves high yield, high efficiency, and high quality in oyster farming through innovative ripening technology, precise nutrient supply, and scientific environmental management, providing a reliable solution for the sustainable development of the oyster industry and possessing broad application prospects. Detailed Implementation

[0029] This invention provides a method for artificially cultivating oysters, comprising the following steps:

[0030] (1) Select oyster parents, ripen them, then artificially inseminate and hatch them to obtain oyster larvae; ripening agents are used during ripening; the ripening agents are: L-acetylcarnitine, marine red yeast extract, brown algae extract, Rehmannia glutinosa extract and placenta extract;

[0031] (2) Cultivate the oyster larvae obtained in step (1), feed them with starter feed, and obtain oyster seedlings. After the oyster seedlings undergo metamorphosis, feed them with artificial oyster feed.

[0032] (3) The oyster seedlings obtained in step (2) are cultured in an intermediate stage to obtain juvenile oysters;

[0033] (4) The juvenile clams are transferred to a breeding farm and raised for 12 to 18 months.

[0034] In this invention, step (4) preferably involves transferring the juvenile clams to a farm for cultivation for 13 to 17 months; more preferably for 14 to 16 months; and even more preferably for 15 months.

[0035] In this invention, step (1) involves temporarily raising oyster parents in an outdoor holding tank for 20-30 days, and then transferring them to an indoor ripening tank for ripening treatment; preferably 22-28 days; more preferably 24-26 days; and even more preferably 25 days.

[0036] In this invention, artificial oyster feed is used during both the temporary holding and ripening stages; both the temporary holding pond and the ripening pond use naturally circulating seawater as the aquaculture water.

[0037] In this invention, the initial feed consists of: *Isochrysis var. *zhanjiangensis*, *Chaetoceros*, *Platycodon*, and *Phaeodactylum tricornutum*; the mass ratio of *Isochrysis var. *zhanjiangensis*, *Chaetoceros*, *Platycodon*, and *Phaeodactylum tricornutum* is 1–3:3–1:3–1:3–1; preferably 2:2:3–1:3–1; further preferably 2:2:2:3–1; and more preferably 1:1:1:1.

[0038] In this invention, during the larval rearing process described in step (2), before the eye spots appear, 3 / 5 of the water is changed daily at a temperature of 21-22°C. After the eye spots appear, the water is changed twice daily, with 1 / 2 of the water changed each time, at a temperature of 24-26°C. Preferably, during the larval rearing process, before the eye spots appear, 3 / 5 of the water is changed daily at a temperature of 21.5°C. After the eye spots appear, the water is changed twice daily, with 1 / 2 of the water changed each time, at a temperature of 25°C.

[0039] In this invention, the proportion of artificial oyster feed fed in step (2) is 4-6% of the larvae's body weight; preferably 5%.

[0040] In step (3), during intermediate rearing, oysters are fed artificial feed at a ratio of 5-7% of their body weight; preferably 6%.

[0041] In this invention, the intermediate cultivation time in step (3) is 65 to 85 days; preferably 70 to 80 days; and more preferably 75 days.

[0042] In this invention, the mass ratio of L-acetylcarnitine, marine red yeast extract, brown algae extract, Rehmannia glutinosa extract, and placenta extract in step (1) is 4-6:9-13:20-30:10-16:10-16; preferably 5:10-12:22-28:11-15:11-15; further preferably 5:11:24-26:12-14:12-14; and even more preferably 5:11:25:13:13.

[0043] This invention provides an artificial oyster farming feed, prepared from the following components in parts by weight: 25-35 parts wheat flour, 8-12 parts extruded soybean flour, 6-10 parts corn flour, 20-40 parts protein raw material, 20-40 parts algae raw material, 3-5 parts calcium dihydrogen phosphate, 2-4 parts zeolite powder, 1-2 parts choline chloride, 2-3 parts compound vitamins, and 0.8-1.2 parts fermentation agent; preferably, 27-33 parts wheat flour, 9-11 parts extruded soybean flour, 7-9 parts corn flour, 25-35 parts protein raw material, 25-35 parts algae raw material, 4 parts calcium dihydrogen phosphate, and 0.8-1.2 parts fermentation agent. The mixture comprises 3 parts stone powder, 2 parts choline chloride, 3 parts compound vitamins, and 0.9-1.1 parts fermentation agent; more preferably, it comprises 29-31 parts flour, 10 parts puffed soybean flour, 8 parts corn flour, 27-33 parts protein raw material, 27-33 parts algae raw material, 4 parts calcium dihydrogen phosphate, 3 parts zeolite powder, 2 parts choline chloride, 3 parts compound vitamins, and 1 part fermentation agent; even more preferably, it comprises 30 parts flour, 10 parts puffed soybean flour, 8 parts corn flour, 30 parts protein raw material, 30 parts algae raw material, 4 parts calcium dihydrogen phosphate, 3 parts zeolite powder, 2 parts choline chloride, 3 parts compound vitamins, and 1 part fermentation agent.

[0044] In this invention, the protein raw materials are: black soldier fly powder, earthworm powder, fly maggot powder, and silkworm pupa powder.

[0045] In this invention, the algae raw materials are: Chaetoceros, Cyclostomata, Seaweed, and Chlorella.

[0046] In this invention, the fermentation agent is: Bacillus coagulans, Bacillus licheniformis, Aspergillus niger, Candida utilis, Pediococcus pentosus, and Lactobacillus rhamnosus.

[0047] In this invention, the compound vitamins are vitamin D, vitamin E, vitamin K, vitamin B2, and vitamin B12; the mass ratio of vitamin D, vitamin E, vitamin K, vitamin B2, and vitamin B12 is 12-16:8-12:12-16:5-9:6-10; preferably 13-15:9-11:13-15:6-8:7-9; more preferably 14:10:14:7:8.

[0048] In this invention, the mass ratio of Bacillus coagulans, Bacillus licheniformis, Aspergillus niger, Candida utilis, Pediococcus pentosus, and Lactobacillus rhamnosus is 16-20:15-17:10-14:7-11:6-10:6-10; preferably 17-19:16:11-13:8-10:7-9:7-9; and more preferably 18:16:12:9:8:8.

[0049] In this invention, the mass ratio of black soldier fly powder, earthworm powder, fly larvae powder, and silkworm pupa powder is 20-30:10-20:5-9:3-7; preferably 22-28:12-18:6-8:4-6; further preferably 24-26:14-16:7:5; and even more preferably 25:15:7:5.

[0050] In this invention, the mass ratio of Chaetoceros, Cyclostomata, Cynostomata and Chlorella is 8-12:8-12:8-12:8-12; preferably 9-11:9-11:9-11:9-11; and more preferably 1:1:1:1.

[0051] The present invention also provides a method for preparing the aforementioned oyster culture feed, comprising the following steps:

[0052] (1) Mix Bacillus coagulans, Bacillus licheniformis, Aspergillus niger and algae raw materials, adjust the moisture content and ferment to obtain material 1;

[0053] (2) Mix the material 1, Candida utilis, Pediococcus pentosus, Lactobacillus rhamnosus and protein raw materials, adjust the moisture content and ferment to obtain material 2;

[0054] (3) Mix the materials 2, flour, puffed soybean flour, corn flour, calcium dihydrogen phosphate, zeolite powder, choline chloride and compound vitamins, granulate and dry to obtain oyster artificial breeding feed.

[0055] In this invention, the fermentation time in steps (1) and (2) is 3 to 7 days; preferably 4 to 6 days; and more preferably 5 days.

[0056] In this invention, the water content is adjusted to 55-75% in steps (1) and (2); preferably 57-73%; more preferably 59-71%; and more preferably 65%.

[0057] In this invention, the particle size of the granulation in step (4) is 50-100 μm; preferably 60-90 μm; more preferably 70-80 μm; and more preferably 80 μm.

[0058] In this invention, the material is dried to a moisture content of ≤8%; preferably 6-8%; more preferably 7%.

[0059] The present invention also provides the application of the oyster artificial culture feed or the oyster artificial culture feed prepared by the preparation method of the oyster artificial culture feed in oyster farming.

[0060] In this invention, the bacterial counts of Bacillus coagulans, Bacillus licheniformis, Aspergillus niger, Candida utilis, Pediococcus pentosus, and Lactobacillus rhamnosus are all 500 million / g.

[0061] In this invention, the Aspergillus niger preservation number is CGMCC No. 8640; Bacillus coagulans GDMCC 1.420; Bacillus licheniformis GDMCC 1.182; Candida utilis CICC 1422; Pediococcus pentosaceus CICC 22726; and Lactobacillus rhamnosus CICC 22825 are all from Shanghai Morning Microbiology.

[0062] In this invention, the L-acetylcarnitine (CAS: 3040-38-8); the marine red yeast extract, prepared by the same method as CN201811018876.3; the brown algae extract from Shaanxi Xintianyu Biotechnology Co., Ltd.; the Rehmannia glutinosa extract from Henan Wokas Biotechnology Co., Ltd.; and the placenta extract from Xi'an Runxue Biotechnology Co., Ltd.

[0063] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0064] Example 1

[0065] An oyster aquaculture feed is prepared from the following components in parts by weight: 25 parts wheat flour, 8 parts puffed soybean flour, 6 parts corn flour, 20 parts protein raw material, 20 parts algae raw material, 3 parts calcium dihydrogen phosphate, 2 parts zeolite powder, 1 part choline chloride, 2 parts compound vitamins, and 0.8 parts fermentation agent.

[0066] The protein raw materials are: black soldier fly powder, earthworm powder, fly larvae powder, and silkworm pupa powder, in a mass ratio of 20:10:5:3;

[0067] The algae raw materials are: Chaetoceros, Cyclostomata, Seaweed, and Chlorella, in a mass ratio of 8:8:8:8.

[0068] The fermentation agent consists of Bacillus coagulans, Bacillus licheniformis, Aspergillus niger, Candida utilis, Pediococcus pentosaceus, and Lactobacillus rhamnosus, in a mass ratio of 16:15:10:7:6:6.

[0069] The compound vitamin consists of vitamin D, vitamin E, vitamin K, vitamin B2, and vitamin B12 in a mass ratio of 12:8:12:5:6.

[0070] The preparation method of the oyster culture feed is as follows:

[0071] (1) Mix Bacillus coagulans, Bacillus licheniformis, Aspergillus niger and algae raw materials, adjust the moisture content to 55% and ferment for 3 days to obtain material 1;

[0072] (2) Mix the material 1, Candida utilis, Pediococcus pentosus, Lactobacillus rhamnosus and protein raw materials, adjust the moisture content to 55% and ferment for 3 days to obtain material 2;

[0073] (3) Mix the material 2, flour, puffed soybean flour, corn flour, calcium dihydrogen phosphate, zeolite powder, choline chloride and compound vitamins, granulate (particle size of 50μm) and dry (moisture content of 8%) to obtain oyster artificial culture feed.

[0074] Example 2

[0075] An oyster aquaculture feed is prepared from the following components in parts by weight: 35 parts wheat flour, 12 parts puffed soybean flour, 10 parts corn flour, 40 parts protein raw material, 40 parts algae raw material, 5 parts calcium dihydrogen phosphate, 4 parts zeolite powder, 2 parts choline chloride, 3 parts compound vitamins, and 1.2 parts fermentation agent.

[0076] The protein raw materials are: black soldier fly powder, earthworm powder, fly larvae powder, and silkworm pupa powder, in a mass ratio of 30:20:9:7;

[0077] The algae raw materials are: Chaetoceros, Cyclostomata, Seaweed, and Chlorella, in a mass ratio of 12:12:12:12.

[0078] The fermentation agent consists of Bacillus coagulans, Bacillus licheniformis, Aspergillus niger, Candida utilis, Pediococcus pentosus, and Lactobacillus rhamnosus, in a mass ratio of 20:17:14:11:10:10.

[0079] The compound vitamin consists of vitamin D, vitamin E, vitamin K, vitamin B2, and vitamin B12 in a mass ratio of 16:12:16:9:10.

[0080] The preparation method of the oyster culture feed is as follows:

[0081] (1) Mix Bacillus coagulans, Bacillus licheniformis, Aspergillus niger and algae raw materials, adjust the moisture content to 75% and ferment for 7 days to obtain material 1;

[0082] (2) Mix the material 1, Candida utilis, Pediococcus pentosus, Lactobacillus rhamnosus and protein raw materials, adjust the moisture content to 75% and ferment for 7 days to obtain material 2;

[0083] (3) Mix the material 2, flour, puffed soybean flour, corn flour, calcium dihydrogen phosphate, zeolite powder, choline chloride and compound vitamins, granulate (particle size of 100μm) and dry (moisture content of 7%) to obtain oyster artificial culture feed.

[0084] Example 3

[0085] An oyster aquaculture feed is prepared from the following components in parts by weight: 30 parts wheat flour, 10 parts puffed soybean flour, 8 parts corn flour, 30 parts protein raw material, 30 parts algae raw material, 4 parts calcium dihydrogen phosphate, 3 parts zeolite powder, 2 parts choline chloride, 3 parts compound vitamins, and 1 part fermentation agent.

[0086] The protein raw materials are: black soldier fly powder, earthworm powder, fly larvae powder, and silkworm pupa powder, in a mass ratio of 25:15:7:5;

[0087] The algae raw materials are: Chaetoceros, Cyclostomata, Seaweed, and Chlorella, in a mass ratio of 10:10:10:10.

[0088] The fermentation agent consists of Bacillus coagulans, Bacillus licheniformis, Aspergillus niger, Candida utilis, Pediococcus pentosus, and Lactobacillus rhamnosus, in a mass ratio of 18:16:12:9:8:8.

[0089] The complex vitamins consist of vitamin D, vitamin E, vitamin K, vitamin B2, and vitamin B12 in a mass ratio of 14:10:14:7:8.

[0090] The preparation method of the oyster culture feed is as follows:

[0091] (1) Mix Bacillus coagulans, Bacillus licheniformis, Aspergillus niger and algae raw materials, adjust the moisture content to 65% and ferment for 5 days to obtain material 1;

[0092] (2) Mix the material 1, Candida utilis, Pediococcus pentosus, Lactobacillus rhamnosus and protein raw materials, adjust the moisture content to 65% and ferment for 8 days to obtain material 2;

[0093] (3) Mix the material 2, flour, puffed soybean flour, corn flour, calcium dihydrogen phosphate, zeolite powder, choline chloride and compound vitamins, granulate (particle size of 80μm) and dry (moisture content of 8%) to obtain oyster artificial culture feed.

[0094] Example 4

[0095] A method for artificially cultivating oysters, the steps are as follows:

[0096] (1) Select oyster parents and place them in an outdoor temporary rearing pond for 20 days. Use naturally circulating seawater as the culture water, with a water temperature of 19℃, salinity of 28, and pH of 8.10. During this period, feed them artificial oyster feed (prepared by the method in Example 1, the same below, with a feeding ratio of 5% of the oyster body weight).

[0097] (2) After temporary rearing, the oysters were transferred to an indoor maturation tank. Natural circulating seawater was used as the aquaculture water, with a water temperature of 22℃, salinity of 28, and pH of 8.11. During the period, the oysters were fed artificial feed (5% of body weight) + maturation agent (mass ratio of 10:1) for maturation treatment. The maturation agent was L-acetylcarnitine, marine red yeast extract, brown algae extract, Rehmannia glutinosa extract and placenta extract, with a mass ratio of 4:9:20:10:10.

[0098] (3) Artificial insemination was performed on the obtained oysters, and after the fertilized eggs hatched, oyster larvae were obtained;

[0099] (4) Oyster larvae are cultured in a water tank and fed with starter feed to obtain oyster seedlings. After the oyster seedlings undergo metamorphosis, they are fed with artificial oyster feed (4% of body weight).

[0100] The initial feed consists of: *Isochrysis galbana*, *Chaetoceros*, *Platycodon grandiflorus*, and *Phaeodactylum tricornutum*, in a mass ratio of 3:1:1:1.

[0101] During the oyster larval rearing process, before the eye spots appear, 3 / 5 of the water is changed daily at a temperature of 21°C. After the eye spots appear, 1 / 2 of the water is changed twice daily at a temperature of 24°C.

[0102] (5) The oyster seedlings were cultured in an outdoor pond for 85 days, using naturally circulating seawater as the culture water and feeding them artificial oyster feed (5% of body weight) to obtain juvenile oysters;

[0103] (6) The obtained juvenile clams are transferred to the breeding farm and cultured on floating rafts for 12 months.

[0104] Example 5

[0105] A method for artificially cultivating oysters, the steps are as follows:

[0106] (1) Select oyster parents and place them in an outdoor temporary rearing pond for 30 days. Use naturally circulating seawater as the aquaculture water, with a water temperature of 25℃, salinity of 32, and pH of 8.22. During this period, feed them artificial oyster feed (prepared by the method in Example 2, the same below, with a feeding ratio of 5% of the oyster body weight).

[0107] (2) After temporary rearing, the oysters were transferred to an indoor maturation tank. Natural circulating seawater was used as the aquaculture water, with a water temperature of 27℃, salinity of 32, and pH of 8.22. During the period, the oysters were fed artificial feed (5% of body weight) + maturation agent (mass ratio of 8:1) for maturation treatment. The maturation agent was L-acetylcarnitine, marine red yeast extract, brown algae extract, Rehmannia glutinosa extract and placenta extract, with a mass ratio of 6:13:30:16:16.

[0108] (3) Artificial insemination was performed on the obtained oysters, and after the fertilized eggs hatched, oyster larvae were obtained;

[0109] (4) Oyster larvae are cultured in a water tank and fed with starter feed to obtain oyster seedlings. After the oyster seedlings undergo metamorphosis, they are fed with artificial oyster feed (6% of body weight).

[0110] The initial feed consists of: *Isochrysis galbana*, *Chaetoceros*, *Platycodon grandiflorus*, and *Phaeodactylum tricornutum*, in a mass ratio of 3:1:1:1.

[0111] During the oyster larval rearing process, before the eye spots appear, 3 / 5 of the water is changed daily at a temperature of 22°C. After the eye spots appear, 1 / 2 of the water is changed twice daily at a temperature of 26°C.

[0112] (5) The oyster seedlings were cultured in an outdoor pond for 85 days, using naturally circulating seawater as the culture water and feeding them artificial oyster feed (7% of body weight) to obtain juvenile oysters;

[0113] (6) The obtained juvenile clams were transferred to the aquaculture farm and cultured on floating rafts for 18 months.

[0114] Example 6

[0115] A method for artificially cultivating oysters, the steps are as follows:

[0116] (1) Select oyster parents and place them in an outdoor temporary rearing pond for 25 days. Use naturally circulating seawater as the aquaculture water, with a water temperature of 23℃, salinity of 30, and pH of 8.20. During this period, feed them artificial oyster feed (prepared by the method in Example 3, the same below, with a feeding ratio of 5% of the oyster body weight).

[0117] (2) After temporary rearing, the oysters were transferred to an indoor maturation tank. Natural circulating seawater was used as the aquaculture water, with a water temperature of 25℃, salinity of 30, and pH of 8.20. During the period, the oysters were fed artificial feed (5% of body weight) + maturation agent (mass ratio of 10:1) for maturation treatment. The maturation agent was L-acetylcarnitine, marine red yeast extract, brown algae extract, Rehmannia glutinosa extract and placenta extract, with a mass ratio of 5:11:25:13:13.

[0118] (3) Artificial insemination was performed on the obtained oysters, and after the fertilized eggs hatched, oyster larvae were obtained;

[0119] (4) Oyster larvae are cultured in a water tank and fed with starter feed to obtain oyster seedlings. After the oyster seedlings undergo metamorphosis, they are fed with artificial oyster feed (5% of body weight).

[0120] The initial feed consists of: *Isochrysis galbana*, *Chaetoceros*, *Platycodon grandiflorus*, and *Phaeodactylum tricornutum*, in a mass ratio of 1:1:1:1.

[0121] During the oyster larval rearing process, before the eye spots appear, 3 / 5 of the water is changed daily at a temperature of 21.5℃. After the eye spots appear, 1 / 2 of the water is changed twice daily at a temperature of 25℃.

[0122] (5) The oyster seedlings were cultivated in an outdoor pond for 75 days, with natural circulating seawater as the culture water and oyster artificial feed (6% of body weight) to obtain juvenile oysters;

[0123] (6) The obtained juvenile clams were transferred to the breeding farm and cultured on floating rafts for 16 months.

[0124] Comparative Example 1

[0125] The other methods are the same as in Example 6, except that no ripening agent is used in step (2).

[0126] Comparative Example 2

[0127] The other methods are the same as in Example 6, except that in step (2), the Rehmannia glutinosa extract and placenta extract are replaced with L-acetylcarnitine in equal amounts.

[0128] Comparative Example 3

[0129] The other methods are the same as in Example 6, except that Chaetoceros, Flat Algae, and Brown Finger Algae are replaced with Isochrysis zhanjiangensis in equal amounts.

[0130] Experimental Example 1

[0131] Four hundred healthy and undamaged oyster parents were selected for an indoor artificial breeding experiment of oysters in Laizhou Bay and were divided into four groups on average.

[0132] The method of Example 6 was used as the experimental group;

[0133] Comparative examples 1, 2, and 3 served as control group 1, control group 2, and control group 3, respectively.

[0134] Each experimental group underwent artificial insemination of the artificially matured oysters. The specific methods were as follows:

[0135] Sperm from males with high sperm motility and eggs from females with high egg maturity were collected for artificial insemination. The sperm and eggs were thoroughly mixed, and microscopic examination revealed a sperm-to-egg ratio of approximately 15:1. After mixing for 20 minutes, the fertilized eggs were washed and transferred to an incubator at a density of 30 eggs / mL.

[0136] 1. During the experiment, relevant indicators, including egg diameter, fertilization rate and hatching rate, were measured in the experimental group, control group 1 and control group 2. The experiment was set up with two replicates and the average value of the results was taken, as shown in Table 1.

[0137] Table 1. Statistics on Egg Diameter, Fertilization Rate, and Hatching Rate

[0138] experimental group 53.38 97.52 93.65 Control group 1 48.23 89.53 83.72 Control group 2 50.66 91.24 88.67

[0139] As shown in Table 1, the eggs obtained by the method of the present invention have a large diameter, a high fertilization rate, and a high hatching rate, which are significantly higher than those of control group 1 and control group 2. This indicates that the ripening agent has achieved the desired results.

[0140] 2. After the fertilized eggs hatched, the shell height and survival rate of oysters at 10, 15, 30 and 60 days of age were recorded in the experimental group and control group 3. The experiment was set up with two replicates, and the average value of the results was taken, as shown in Tables 2 and 3.

[0141] Table 2 Shell Height Statistics

[0142] experimental group 126.8μm 212.5μm 2.1mm 13.4mm Control group 3 105.4μm 187.4μm 1.8mm 11.7mm

[0143] Table 3 Survival Rate Statistics

[0144] experimental group 93.5％ 90.2％ 85.2％ 73.8％ Control group 3 91.4％ 84.7％ 78.4％ 65.5％

[0145] It can be seen that the oysters cultured by the method of this invention have a faster growth rate and a higher survival rate, with all data being superior to the control group 3. However, failure to use the starter feed provided by this invention significantly affects the growth of the oysters.

[0146] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A method for artificially cultivating oysters, characterized in that, Includes the following steps: (1) Select oyster parents, ripen them, then artificially inseminate and hatch them to obtain oyster larvae; ripening agents are used during ripening; the ripening agents are: L-acetylcarnitine, marine red yeast extract, brown algae extract, Rehmannia glutinosa extract and placenta extract; (2) Cultivate the oyster larvae obtained in step (1), feed them with starter feed, and obtain oyster seedlings. After the oyster seedlings undergo metamorphosis, feed them with artificial oyster feed. (3) The oyster seedlings obtained in step (2) are cultured in an intermediate stage to obtain juvenile oysters; (4) The juvenile clams are transferred to a breeding farm and raised for 12 to 18 months; The initial feed consists of: *Isotrichum chinense*, *Chaetoceros*, *Platycodon grandiflorus*, and *Phaeodactylum tricornutum*; the mass ratio of *Isotrichum chinense*, *Chaetoceros*, *Platycodon grandiflorus*, and *Phaeodactylum tricornutum* is 1~3:3~1:3~1:3~1. The mass ratio of L-acetylcarnitine, marine red yeast extract, brown algae extract, prepared rehmannia root extract, and placenta extract in step (1) is 4~6:9~13:20~30:10~16:10~16; The artificial oyster feed is prepared from the following components in parts by weight: 25-35 parts wheat flour, 8-12 parts puffed soybean flour, 6-10 parts corn flour, 20-40 parts protein raw material, 20-40 parts algae raw material, 3-5 parts calcium dihydrogen phosphate, 2-4 parts zeolite powder, 1-2 parts choline chloride, 2-3 parts compound vitamins, and 0.8-1.2 parts fermentation agent; the protein raw material is: black soldier fly powder, earthworm powder, fly larvae powder, and silkworm pupa powder; the algae raw material is: Chaetoceros, Cyclosporium, Seaweed, and Chlorella; the fermentation agent is: Bacillus coagulans, Bacillus licheniformis, Aspergillus niger, Candida utilis, Pediococcus pentosaceus, and Lactobacillus rhamnosus; The compound vitamins are vitamin D, vitamin E, vitamin K, vitamin B2 and vitamin B12; the mass ratio of vitamin D, vitamin E, vitamin K, vitamin B2 and vitamin B12 is 12~16:8~12:12~16:5~9:6~10; The mass ratio of Bacillus coagulans, Bacillus licheniformis, Aspergillus niger, Candida utilis, Pediococcus pentosus, and Lactobacillus rhamnosus is 16~20:15~17:10~14:7~11:6~10:6~10; The mass ratio of black soldier fly powder, earthworm powder, fly larvae powder, and silkworm pupa powder is 20~30:10~20:5~9:3~7; the mass ratio of Chaetoceros, Cyclostomata, Seaweed, and Chlorella is 8~12:8~12:8~12:8~12.

2. The method for artificially cultivating oysters according to claim 1, characterized in that, Step (1) The oyster parent stock is temporarily kept in an outdoor holding tank for 20-30 days, and then moved to an indoor ripening tank for ripening treatment.

3. The method for artificially cultivating oysters according to claim 2, characterized in that, During both the temporary rearing and ripening stages, artificial oyster feed was used; both the temporary rearing and ripening ponds used naturally circulating seawater as the aquaculture water.

4. The method for artificially cultivating oysters according to claim 1, characterized in that, During the larval rearing process described in step (2), before the eye spots appear, change 3 / 5 of the water every day at a water temperature of 21-22℃. After the eye spots appear, change 1 / 2 of the water twice a day at a water temperature of 24-26℃.

5. The method for artificially cultivating oysters according to claim 1, characterized in that, In step (2), the proportion of artificial oyster feed fed is 4-6% of the larvae's body weight; in step (3), artificial oyster feed is fed during intermediate rearing, with a feeding ratio of 5-7% of the larvae's body weight.

6. The method for artificially cultivating oysters according to claim 1, characterized in that, The intermediate cultivation time in step (3) is 65~85 days.