Bone-strengthening and foundation-consolidating soup as well as preparation method and application thereof

Abstract

The invention relates to a bone-strengthening and foundation-consolidating soup as well as a preparation method and application thereof. The bone-strengthening and foundation-consolidating soup is prepared from the following traditional Chinese medicines: codonopsis pilosula, angelica sinensis, spina date seeds, ligusticum wallichii, schisandra chinensis, polygala tenuifolia, rhizoma acori graminei, semen cuscutae, radix rehmanniae recen and cortex lycii radicis. The bone strengthening and root consolidating decoction has the beneficial effects that the bone mass, the bone mineral density and the bone mineral content can be remarkably improved, the quantity of bone trabecula is increased, and the bone strengthening and root consolidating decoction has a positive curative effect on prevention and treatment of osteoporosis.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine, and in particular to a bone strengthening and body strengthening soup, a preparation method and an application thereof. Background Art

[0002] Osteoporosis is a systemic bone disease characterized by decreased bone mass and deterioration of bone microarchitecture, significantly increasing the risk of fractures. Traditional treatment relies primarily on calcium supplements, vitamin D supplements, and bisphosphonates. However, long-term use can cause side effects such as gastrointestinal reactions and osteonecrosis of the jaw, and patient compliance is generally low.

[0003] Traditional Chinese Medicine (TCM) attributes osteoporosis to "kidney essence deficiency and bone marrow malnutrition." Prescriptions that tonify the kidneys and strengthen bones, such as Liuwei Dihuang Wan (Liuwei Dihuang Wan) and Gusongbao (Gusongbao), are commonly used clinically. However, existing compound prescriptions often focus solely on kidney or calcium supplementation, resulting in several drawbacks: slow efficacy and inability to rapidly inhibit bone loss; ingredient combinations that fail to adequately nourish the spleen and kidneys, impairing nutrient absorption and bone formation efficiency; and a lack of synergistic mechanisms designed to repair bone microstructure, leaving the overall efficacy of these prescriptions in need of improvement. Summary of the Invention

[0004] The purpose of the present invention is to provide a bone strengthening and body strengthening soup and its preparation method and application, so as to solve the above problems existing in the prior art.

[0005] The technical solution of the present invention to solve the above technical problems is as follows: A bone strengthening and foundation-building soup is prepared from the following Chinese medicinal materials: codonopsis pilosula, angelica sinensis, spinach jujube seeds, ligusticum chuanxiong, schisandra chinensis, polygala tenuifolia, calamus tatarinowii, dodder seed, raw rehmannia root and lycium bark.

[0006] The beneficial effects of the present invention are as follows: it improves bone metabolism by tonifying the kidneys and replenishing essence, strengthening the spleen and replenishing qi, and promoting blood circulation and unblocking meridians. The ingredient combination takes into account the basic principle of "tonifying both the spleen and kidneys." The kidney-tonifying herbs Cuscuta chinensis, Rehmannia root, Schisandra chinensis, and Lycium bark primarily maintain bone metabolism balance by promoting osteoblast differentiation and inhibiting osteoclast formation. The spleen-tonifying herbs Codonopsis pilosula, Angelica sinensis, and Ziziphus jujuba seeds primarily enhance bone metabolism by promoting calcium absorption, improving muscle strength, and regulating immunity. The synergistically enhancing herbs Chuanxiong rhizome, Polygala tenuifolia, and Acorus tatarinowii indirectly affect bone metabolism by promoting blood circulation and removing blood stasis, improving bone microcirculation, and regulating the neuro-endocrine axis. Qiang Gu Gu Ben Tang can significantly increase bone mass, bone density, bone mineral content, and increase the number of trabeculae, showing a positive therapeutic effect in the prevention and treatment of osteoporosis.

[0007] On the basis of the above technical solution, the present invention can also be improved as follows.

[0008] Furthermore, the bone strengthening and foundation strengthening soup is prepared from the following traditional Chinese medicines in parts by weight: 10-20 parts of Codonopsis pilosula, 10-20 parts of Angelica sinensis, 10-20 parts of Ziziphus jujuba seeds, 10-20 parts of Ligusticum chuanxiong, 10-20 parts of Schisandra chinensis, 10-20 parts of Polygala tenuifolia, 10-20 parts of Acorus calamus, 10-20 parts of Cuscuta chinensis, 10-20 parts of Rehmannia glutinosa, and 10-20 parts of Lycium bark.

[0009] Furthermore, the Qiang Gu Gu Ben soup is a decoction.

[0010] Another technical solution of the present invention is as follows: A preparation method of a bone strengthening and foundation-building soup is used for preparing the bone strengthening and foundation-building soup.

[0011] The preparation method comprises the following steps: Step 1: Place the prescribed amount of Codonopsis pilosula, Angelica sinensis, Ziziphus jujuba seeds, Chuanxiong rhizome, Schisandra chinensis, Polygala tenuifolia, Acorus gramineus, Cuscuta chinensis, Rehmannia root and Cortex Lycii into a decoction container, add an appropriate amount of water, soak, boil over high heat, then boil over low heat, filter to obtain a first decoction, and pour the first decoction into a container; Step 2: Add 4 times the weight of water to the filtered medicinal residue, boil over high heat, then simmer over low heat, and filter to obtain a second decoction; combine the two decoctions to concentrate the two decoctions. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 The skeletal structure of Kunming mice's tibia and the horizontal cross-section of the mouse's tibia; Figure 2 is a histogram of bone volume fraction in Kunming mice; Figure 3 is a bar graph of bone mineral density in Kunming mice; Figure 4 is a bar graph of bone mineral content in Kunming mice; Figure 5 This is a bar graph of the number of trabeculae in Kunming mice. DETAILED DESCRIPTION

[0013] The principles and features of the present invention are described below with reference to the accompanying drawings. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.

[0014] Example 1 A bone strengthening and foundation-building soup is prepared from the following Chinese medicinal materials: codonopsis pilosula, angelica sinensis, spinach jujube seeds, ligusticum chuanxiong, schisandra chinensis, polygala tenuifolia, calamus tatarinowii, dodder seed, raw rehmannia root and lycium bark.

[0015] This invention improves bone metabolism by tonifying the kidneys and replenishing essence, strengthening the spleen and replenishing qi, and promoting blood circulation and unblocking meridians. The ingredient combination takes into account the fundamental principle of "tonifying both the spleen and kidneys." Kidney-tonifying herbs like Cuscuta chinensis, Rehmannia root, Schisandra chinensis, and Lycium bark primarily maintain bone metabolism balance by promoting osteoblast differentiation and inhibiting osteoclast formation. Spleen-tonifying herbs like Codonopsis pilosula, Angelica sinensis, and Ziziphus jujuba seeds primarily enhance bone metabolism by promoting calcium absorption, improving muscle strength, and regulating immunity. The synergistically enhancing herbs like Chuanxiong rhizome, Polygala tenuifolia, and Acorus tatarinowii indirectly affect bone metabolism by promoting blood circulation and removing blood stasis, improving bone microcirculation, and regulating the neuroendocrine axis.

[0016] Codonopsis pilosula replenishes Qi and strengthens the spleen, benefits the lungs and produces body fluids. It can improve spleen and stomach function, promote nutrient absorption, and enhance the Qi and blood biochemical processes required for bone metabolism. Codonopsis pilosula polysaccharides can promote osteoblast proliferation and inhibit osteoclast activity.

[0017] Angelica sinensis replenishes Qi and activates blood circulation, regulates menstruation and relieves pain. It can improve bone malnutrition caused by blood deficiency, promote bone marrow hematopoiesis, and enhance bone nutrient supply. It contains ferulic acid, which can inhibit osteoclast differentiation and reduce bone loss.

[0018] Ziziphus jujuba seeds nourish the heart and calm the mind, reduce sweating, and promote salivation. They can also improve insomnia caused by kidney deficiency (the kidneys govern bones, and long-term insomnia can worsen osteoporosis). They also contain saponins, which can indirectly affect bone metabolism by regulating the hypothalamic-pituitary-adrenal axis (HPA axis). Chuanxiong promotes blood circulation and qi, dispels wind and relieves pain. It can promote local blood circulation, improve the bone microenvironment, and reduce the risk of avascular necrosis of bone. It also contains ligustrazine, which inhibits inflammatory factors (such as TNF-α (tumor necrosis factor) and IL-6 (interleukin-6)) and reduces inflammatory bone destruction. Schisandra chinensis has astringent properties, replenishes Qi and promotes fluid production, and nourishes the kidneys and calms the mind. It can indirectly enhance bone health by tonifying the kidneys (the kidneys govern bones). It also contains lignans, which have antioxidant properties and reduce free radical damage to bone tissue. Polygala root soothes the mind, improves intelligence, dispels phlegm, and stimulates the mind. It can improve heart-kidney disharmony (long-term anxiety and insomnia can worsen osteoporosis) and indirectly influence bone metabolism by regulating the neuro-endocrine system. Acorus calamus can open the mind and dispel phlegm, refreshing the mind and improving intelligence. It can improve abnormal bone metabolism caused by phlegm-dampness stagnation (such as obesity-related osteoporosis) and can affect calcium absorption by regulating the intestinal flora.

[0019] Cuscuta seed nourishes the kidneys and improves essence, nourishes the liver, and improves eyesight. As a core kidney-tonifying herb, it directly enhances kidney essence (the kidneys are responsible for bone and marrow production). Cuscuta seed flavonoids can promote osteoblast differentiation and inhibit osteoclast activity. Rehmannia root clears heat and cools the blood, nourishing yin and promoting fluid production. It can improve osteoporosis caused by yin deficiency and internal heat (such as bone loss in menopausal women due to decreased estrogen levels). It also contains iridoids, which can affect bone metabolism by regulating estrogen receptors. Rehmannia root bark cools the blood, reduces steam, clears the lungs, and reduces internal heat. It can improve osteoporosis caused by yin deficiency and excessive internal heat (such as bone loss due to hyperthyroidism and diabetes). It contains goji berries, which inhibit osteoclast activity and reduce bone resorption.

[0020] Qiang Gu Gu Ben Tang can significantly increase bone mass, bone density, bone mineral content, and increase the number of trabeculae, and has a positive therapeutic effect on the prevention and treatment of osteoporosis.

[0021] Example 2 This embodiment is a further improvement on the basis of embodiment 1, and the details are as follows: The bone strengthening and foundation-strengthening soup is prepared from the following traditional Chinese medicines in parts by weight: 10-20 parts of Codonopsis pilosula, 10-20 parts of Angelica sinensis, 10-20 parts of Ziziphus jujuba seeds, 10-20 parts of Chuanxiong, 10-20 parts of Schisandra chinensis, 10-20 parts of Polygala tenuifolia, 10-20 parts of Acorus calamus, 10-20 parts of Cuscuta chinensis, 10-20 parts of Rehmannia glutinosa, and 10-20 parts of Lycium bark.

[0022] The bone strengthening and foundation-strengthening soup is prepared from the following traditional Chinese medicines in parts by weight: 12-18 parts of Codonopsis pilosula, 12-18 parts of Angelica sinensis, 12-18 parts of Ziziphus jujuba seeds, 10-20 parts of Chuanxiong, 12-18 parts of Schisandra chinensis, 12-18 parts of Polygala tenuifolia, 12-18 parts of Acorus calamus, 12-18 parts of Cuscuta chinensis, 12-18 parts of Rehmannia glutinosa, and 12-18 parts of Lycium bark.

[0023] Qiang Gu Gu Ben Tang is a decoction.

[0024] Example 3 A method for preparing a bone strengthening and body strengthening soup, for preparing the above-mentioned bone strengthening and body strengthening soup, comprising the following steps: Step 1: Place the prescribed amount of Codonopsis pilosula, Angelica sinensis, Ziziphus jujuba seeds, Chuanxiong rhizome, Schisandra chinensis, Polygala tenuifolia, Acorus gramineus, Cuscuta chinensis, Rehmannia root and Cortex Lycii into a decoction container, add an appropriate amount of water, soak, boil over high heat, then boil over low heat, filter to obtain a first decoction, and pour the first decoction into a container; Step 2: Add 4 times the weight of water to the filtered medicinal residue, boil over high heat, then simmer over low heat, and filter to obtain a second decoction; combine the two decoctions to concentrate the two decoctions.

[0025] When specifically implemented, step 1, the prescription amount of Codonopsis pilosula, Angelica sinensis, Ziziphus jujuba seed, Chuanxiong, Schisandra chinensis, Polygala tenuifolia, Acorus gramineus, Cuscuta chinensis, Rehmannia glutinosa and Lycium bark are placed in a decoction container, 4-8 times the weight of water is added, soak for 30 minutes, boil over high heat, then simmer for 20 minutes, filter the first decoction, and pour the first decoction into the container; Step 2: add 4 times the weight of water to the filtered medicinal residue, boil over high heat, then simmer over low heat for 20 minutes, filter to obtain a second decoction; combine the two decoctions and concentrate the two decoctions to 100 ml.

[0026] Example 4 The invention discloses an application of a bone strengthening and foundation-building soup, which is used for preventing and treating osteoporosis. Specific embodiment 1 Preparation of Qianggu Guben Decoction Step 1: According to the prescription, place 15 parts of Codonopsis pilosula, 15 parts of Angelica sinensis, 15 parts of Ziziphus jujuba seeds, 15 parts of Chuanxiong, 15 parts of Schisandra chinensis, 15 parts of Polygala tenuifolia, 15 parts of Acorus gramineus, 15 parts of Cuscuta chinensis, 15 parts of Rehmannia glutinosa, and 15 parts of Lycium bark into a decoction container, add 4-8 times the weight of water, soak for 30 minutes, boil over high heat, then simmer for 20 minutes over low heat, filter to obtain the first decoction, and pour the first decoction into a container; Step 2: add 4 times the weight of water to the filtered medicinal residue, boil over high heat, then simmer over low heat for 20 minutes, filter to obtain a second decoction; combine the two decoctions and concentrate the two decoctions to 100 ml.

[0028] Establishing animal models Seventeen 8-week-old male Kunming mice were randomly divided into three groups: a normal group (Control, 5 mice), a model group (Model, Mod, 7 mice), and a Qianggu Guben Tang Intervention (QGTI) group (5 mice). They were adaptively raised for one week. The Kunming mice in the model group and the Qianggu Guben Decoction intervention group were injected subcutaneously with D-galactose 120 mg / kg / day combined with sodium nitrite 90 mg / kg / day at the back of the neck, while the normal group was injected subcutaneously with an equal volume of normal saline at the back of the neck, and the injection was continued for 56 days.

[0029] The Kunming mice in the Qianggu Guben Decoction intervention group were given Qianggu Guben Decoction 19.5 g / kg / day (0.52 ml) by gavage at the beginning of the animal model establishment for 56 days. The Kunming mice in the model group were gavaged with an equal volume of normal saline for 56 days; Kunming mice in the normal group were not treated; After 56 days, all Kunming mice were sacrificed under anesthesia, and the left hind legs of mice in the normal group, model group, and Qianggu Guben Decoction intervention group were taken for MicroCT scanning and data analysis.

[0030] Data Analysis Multi-angle scans of the isolated left leg of a mouse were performed using a micro-CT (microcomputed tomography) scanner (Pingsheng Healthcare, China). The sample was placed between the X-ray source and a CMOS image sensor. During the scan, the sample was rotated 360° along its longitudinal axis while remaining within the scanning area. Images were captured by the CMOS image sensor and transmitted to a computer equipped with image processing software. The "Large In Vitro Standard Scan" protocol was selected, with a tube voltage of 90 kV, a tube current of 0.05 mA, and a lateral field of view (FOV) of 30 mm. Two bed positions were selected, and the scan was initiated by selecting the appropriate position. The total scan time was 15 minutes per sample. Reconstruction Process: 3D reconstruction of the sample (XX) was performed using the FDK reconstruction algorithm / type. The reconstruction dimensions were 2K*2K*2K, and the reconstructed pixels were 0.015*0.015*0.017 mm. Analysis Process: Image segmentation of the experimental results was performed using Avatar 1.6.9.3. The tibial plateau was located and 100 CT slices were taken 0.5 mm below the tibial plateau as the region of interest for analysis. A minimum CT threshold of 1200 was used to obtain a trabecular bone mask (BV) and medullary cavity mask (TV) for bone parameter analysis.

[0031] result: like Figure 1 As shown, the coronal plane shows the structure of the mouse tibia from front to back, and the anterior and posterior morphology and structural characteristics of the bone can be clearly observed. The cross-section shows the horizontal section of the mouse tibia, which can observe the cross-sectional morphology of the bone in detail, including the arrangement of trabeculae and the thickness of cortical bone. Compared with the normal group (Ctrl), the bones of the model group (Mod) mice after β-galactose induction treatment showed typical characteristics of osteoporosis, including a decrease in the number of trabeculae, thinning of trabecular thickness, increased trabecular separation, and reduced cortical bone thickness. Compared with the model group (Mod), the bone structure of the mice in the Qiang Gu Gu Ben Tang intervention group (QGTI) was improved, the number and thickness of trabeculae increased, the trabecular separation decreased, and the cortical bone thickness was restored, indicating that Qiang Gu Gu Ben Tang has a certain intervention effect on osteoporosis; like Figure 2-5 As shown in Figure 2, these parameters include bone volume fraction (Tb.BV / TV), bone mineral density (Tb.BMD), bone mineral content (Tb.BMC), and trabecular number (Tb.N). These parameters are important in the diagnosis, evaluation, and treatment of osteoporosis.

[0032] Compared with the normal group (Ctrl), the bone volume fraction (Tb.BV / TV) of the model group (Mod) was significantly reduced after osteoporosis induction, indicating a significant decrease in bone mass and bone density; bone mineral density (Tb.BMD) was also significantly reduced, further confirming the significant decrease in bone density; bone mineral content (Tb.BMC) was also significantly reduced, indicating a significant decrease in mineral content; and the number of trabeculae (Tb.N) was also significantly reduced, indicating that the trabecular structure was destroyed.

[0033] In contrast, the bone volume fraction (Tb.BV / TV), bone mineral density (Tb.BMD), bone mineral content (Tb.BMC) and trabecular number (Tb.N) of the Qianggu Guben Decoction intervention group (QGTI) were significantly better than those of the model group (Mod), indicating that Qianggu Guben Decoction has a significant preventive and ameliorative effect on osteoporosis.

[0034] In summary, Qiang Gu Gu Ben Tang can significantly increase bone mass, bone density, bone mineral content, and increase the number of trabeculae, and has a positive therapeutic effect on the prevention and treatment of osteoporosis.

[0035] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A bone strengthening and body strengthening soup, characterized in that: The bone strengthening and foundation-strengthening soup is prepared from the following Chinese medicinal materials: codonopsis pilosula, angelica sinensis, spinach seed, ligusticum chuanxiong, schisandra chinensis, polygala tenuifolia, calamus tatarinowii, dodder seed, raw rehmannia root and lycium bark.

2. The bone strengthening soup according to claim 1, characterized in that The bone strengthening and foundation strengthening soup is prepared from the following traditional Chinese medicines in parts by weight: 10-20 parts of Codonopsis pilosula, 10-20 parts of Angelica sinensis, 10-20 parts of Ziziphus jujuba seeds, 10-20 parts of Ligusticum chuanxiong, 10-20 parts of Schisandra chinensis, 10-20 parts of Polygala tenuifolia, 10-20 parts of Acorus calamus, 10-20 parts of Cuscuta chinensis, 10-20 parts of Rehmannia root, and 10-20 parts of Lycium bark.

3. The bone strengthening soup according to claim 1, characterized in that The bone strengthening and foundation strengthening soup is prepared from the following traditional Chinese medicines in parts by weight: 12-18 parts of Codonopsis pilosula, 12-18 parts of Angelica sinensis, 12-18 parts of Ziziphus jujuba seeds, 10-20 parts of Ligusticum chuanxiong, 12-18 parts of Schisandra chinensis, 12-18 parts of Polygala tenuifolia, 12-18 parts of Acorus calamus, 12-18 parts of Cuscuta chinensis, 12-18 parts of Rehmannia root, and 12-18 parts of Lycium bark.

4. The bone strengthening soup according to claim 1, characterized in that The Qiang Gu Gu Ben soup is a decoction.

5. A method for preparing a bone strengthening soup, characterized in that: Used to prepare the bone strengthening soup as described in any one of claims 1 to 4.

6. The method for preparing the bone strengthening soup according to claim 5, characterized in that: The preparation method comprises the following steps: Step 1: Place the prescribed amount of Codonopsis pilosula, Angelica sinensis, Ziziphus jujuba seeds, Chuanxiong rhizome, Schisandra chinensis, Polygala tenuifolia, Acorus gramineus, Cuscuta chinensis, Rehmannia root and Cortex Lycii into a decoction container, add an appropriate amount of water, soak, boil over high heat, then boil over low heat, filter to obtain a first decoction, and pour the first decoction into a container; Step 2: Add 4 times the weight of water to the filtered medicinal residue, boil over high heat, then simmer over low heat, and filter to obtain a second decoction; combine the two decoctions to concentrate the two decoctions.

7. An application of a bone strengthening and body strengthening soup, characterized in that: The Qiang Gu Gu Ben decoction according to any one of claims 1 to 4 is used to prevent and treat osteoporosis.

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