A method for cultivating gamma-aminobutyric acid-enriched carex fructicosus sprout

Abstract

The present application relates to the technical field of Salicornia bigelovii cultivation, and discloses a method for cultivating Salicornia bigelovii rich in gamma-aminobutyric acid, which comprises the following steps: selecting Salicornia bigelovii seeds, soaking the seeds with clean water, and then disinfecting the seeds with sodium hypochlorite solution to obtain treated Salicornia bigelovii seeds; sowing the treated Salicornia bigelovii seeds, covering the seeds with a layer of gauze after sowing, and carrying out first-stage cultivation; carrying out second-stage cultivation after the first-stage cultivation is completed, and harvesting the Salicornia bigelovii when the sprouts grow to a height of 8-10 cm to obtain Salicornia bigelovii rich in gamma-aminobutyric acid. The present application makes full use of the nutritional characteristics of Salicornia bigelovii, and the cultivated Salicornia bigelovii is rich in gamma-aminobutyric acid, protein, dietary fiber, vitamins and minerals, has high nutritional value, and can meet the demand of people for healthy food.

Description

Technical Field

[0001] This invention relates to the field of *Solanum tuberosum* sprout cultivation technology, and more specifically, to a method for cultivating *Solanum tuberosum* sprouts rich in γ-aminobutyric acid (GABA). Background Technology

[0002] Sand rice (Agriophyllum squarrosum) is a typical drought- and salt-tolerant plant, widely distributed in arid and semi-arid regions. Its seeds are rich in nutrients, including protein, dietary fiber, unsaturated fatty acids, various vitamins, and minerals, and have high nutritional value.

[0003] With increasing health awareness, the demand for healthy foods is growing, and sand rice and its derivative products are gradually coming into the public eye. However, traditional sand rice cultivation methods are limited by environmental conditions, resulting in low yields and low utilization of the seeds after harvest, failing to fully realize its potential value.

[0004] Sprouts, as a highly nutritious and short-cycle vegetable, are very popular with consumers and have a broad market prospect. However, as of now, there are no reports on the cultivation technology of *Solanum tuberosum* sprouts. Gamma-aminobutyric acid (GABA), as an important functional component, has various physiological functions such as lowering blood pressure, calming the nerves, and improving sleep.

[0005] Based on this, a method for cultivating *Salvia sponge* sprouts rich in γ-aminobutyric acid is provided, which has important economic and social value for making full use of *Salvia sponge* resources and meeting people's demand for healthy food. Summary of the Invention

[0006] In view of this, the present invention proposes a method for cultivating *Solanum tuberosum* sprouts rich in γ-aminobutyric acid (GABA), aiming to solve at least one of the aforementioned background technical problems.

[0007] This invention proposes a method for cultivating *Solanum tuberosum* sprouts rich in γ-aminobutyric acid (GABA), comprising the following steps:

[0008] Select sand rice seeds, soak them in clean water, and then disinfect them with sodium hypochlorite solution to obtain treated sand rice seeds;

[0009] The treated sand rice seeds were sown in a culture medium, and then covered with a thin gauze for the first stage of cultivation.

[0010] After the first stage of cultivation is completed, the second stage of cultivation is carried out. When the sprouts grow to 8-10 cm in height, they are harvested to obtain sand pea sprouts rich in γ-aminobutyric acid.

[0011] Preferably, the soaking time is 6 to 8 hours.

[0012] Preferably, the disinfection specifically involves disinfecting the sand rice seeds with a 0.1% sodium hypochlorite solution for 10 minutes, and then rinsing them thoroughly with clean water.

[0013] Preferably, the concentration of *Salix matsudana* seeds during sowing is 4-5 seeds / cm³. 2

[0014] Preferably, the conditions for the first stage of cultivation are: temperature of 20-25℃, humidity of 80%-90%, and shading treatment.

[0015] Preferably, the conditions for the second stage of cultivation are: temperature of 15-25℃, humidity of 70%-80%, and sunlight exposure.

[0016] Preferably, both the first and second stage cultivation stages use neutral purified water for spraying, with the pH value controlled between 6.5 and 7.0. The spraying frequency is once every 2 days, and the spraying volume is 200-300 mL / m³. 2 .

[0017] Preferably, the culture medium is a mixture of coconut coir, perlite and vermiculite in a ratio of 3:1:1.

[0018] Preferably, the selection of sand rice seeds specifically refers to selecting sand rice seeds that are plump and free from pests and diseases.

[0019] The present invention also provides the application of the above-described method for cultivating *Solanum tuberosum* sprouts rich in γ-aminobutyric acid in the cultivation of *Solanum tuberosum* sprouts.

[0020] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0021] (1) This invention makes full use of the nutritional characteristics of sand rice. The sand rice sprouts cultivated are rich in γ-aminobutyric acid, as well as protein, dietary fiber, vitamins and minerals. They have high nutritional value and can meet people's demand for healthy food.

[0022] (2) The present invention uses soilless cultivation technology and uses a specific ratio of coconut coir, perlite and vermiculite as cultivation substrate, which avoids soil pollution and improves the hygiene and safety of sprouts.

[0023] (3) By optimizing the cultivation environment, such as temperature, humidity, light and other conditions, and reasonable water control, this invention shortens the growth cycle of sand sprigs, increases yield, and thus improves economic benefits.

[0024] (4) The method of the present invention is simple and easy to understand, and the parameters of each step are clear. It is suitable for large-scale production and has good prospects for promotion. Attached Figure Description

[0025] Various other advantages and benefits will become apparent to those skilled in the art upon reading the following detailed description of preferred embodiments. The accompanying drawings are for illustrative purposes only and are not intended to limit the invention. Furthermore, the same reference numerals denote the same parts throughout the drawings. In the drawings:

[0026] Figure 1 This is a flowchart of the method for cultivating *Solanum tuberosum* sprouts rich in γ-aminobutyric acid (GABA) according to the present invention. Detailed Implementation

[0027] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention. It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the present invention.

[0028] Furthermore, regarding the numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Every smaller range between any stated value or intermediate value within a stated range, and any other stated value or intermediate value within said range, is also included within this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0029] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0030] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0031] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0032] This invention provides a method for cultivating *Solanum tuberosum* sprouts rich in γ-aminobutyric acid (GABA), characterized by comprising the following steps:

[0033] Select sand rice seeds, soak them in clean water, and then disinfect them with sodium hypochlorite solution to obtain treated sand rice seeds;

[0034] The treated sand rice seeds were sown in a culture medium, and then covered with a thin gauze for the first stage of cultivation.

[0035] After the first stage of cultivation is completed, the second stage of cultivation is carried out. When the sprouts grow to 8-10 cm in height, they are harvested to obtain sand pea sprouts rich in γ-aminobutyric acid.

[0036] Select sand rice seeds, soak them in clean water, and then disinfect them with sodium hypochlorite solution to obtain treated sand rice seeds;

[0037] High-quality Shami seeds are selected. The Shami strain is one of the varieties from Minqin, Ulan Buhe, Naiman, Erxiang, and Haolaihure. After soaking, screening, and disinfection, the seeds are given a good foundation for subsequent cultivation. Soaking allows the seeds to absorb enough water, which is beneficial for germination; screening removes inferior seeds and ensures seed quality; and disinfection reduces the impact of pests and diseases.

[0038] In this invention, the soaking time is preferably 6 to 8 hours.

[0039] In this invention, the disinfection is preferably carried out by disinfecting the seeds of *Salix matsudana* with a 0.1% sodium hypochlorite solution for 10 minutes, and then rinsing them with clean water.

[0040] The treated sand rice seeds were sown, and then covered with a thin gauze for the first stage of cultivation.

[0041] After the first stage of cultivation is completed, the second stage of cultivation is carried out. When the sprouts grow to 8-10 cm in height, they are harvested to obtain sand pea sprouts rich in γ-aminobutyric acid.

[0042] Sow the treated seeds at a certain density in the cultivation trays, and cover them with gauze about 0.5 cm thick to create a suitable germination and growth environment. Temperature, humidity, and light conditions are specifically controlled at different growth stages to promote healthy growth of the sprouts. Shading during the initial cultivation stage is beneficial for seed germination, while subsequent light exposure is sufficient to meet the needs of photosynthesis.

[0043] In this invention, the preferred concentration of *Salix matsutake* seeds during sowing is 4-5 seeds / cm³. 2

[0044] In this invention, the preferred conditions for the first stage of cultivation are: a temperature of 20-25°C, a humidity of 80%-90%, and shading treatment.

[0045] The first stage of cultivation of the corresponding sand rice seeds in this invention takes place from day 1 to day 3.

[0046] In this invention, the preferred conditions for the second stage of cultivation are: a temperature of 15-25°C, a humidity of 70%-80%, and sunlight exposure.

[0047] The second stage of cultivation of the corresponding sand rice seeds in this invention takes place from day 4 to day 7 after germination.

[0048] In this invention, both the first and second stage cultivation stages utilize neutral purified water spraying, with the pH value controlled between 6.5 and 7.0. The spraying frequency is once every 2 days, and the spraying volume is 200–300 mL / m³. 2 .

[0049] Use neutral purified water for spraying, and control the pH value, spraying frequency and spraying amount to provide suitable water conditions for the growth of sprouts and avoid affecting the growth and quality of sprouts due to improper water.

[0050] In this invention, the culture medium is preferably a mixture of coconut coir, perlite and vermiculite in a ratio of 3:1:1.

[0051] In this invention, the selection of sand rice seeds specifically refers to selecting sand rice seeds that are plump and free from pests and diseases.

[0052] The present invention also provides the application of the above-described method for cultivating *Solanum tuberosum* sprouts rich in γ-aminobutyric acid in the cultivation of *Solanum tuberosum* sprouts.

[0053] Example 1

[0054] (1) Select high-quality sand rice seeds of the Minqin strain, soak them in clean water for 6 hours, remove inferior seeds floating on the surface of the water during this time, then disinfect them with 0.1% sodium hypochlorite solution for 10 minutes, and then rinse them clean with water.

[0055] (2) Prepare the culture substrate by mixing coconut coir, perlite and vermiculite in a ratio of 3:1:1. Put the sterilized culture substrate into the culture tray, and then sow the treated seeds evenly on the culture substrate at a density of 4 seeds per square centimeter. Cover with a thin gauze of about 0.5 cm and press it down gently.

[0056] (3) In the early stage of cultivation (1-3 days), the ambient temperature is controlled at 22℃ and the humidity is kept at 85%. Shading treatment is carried out to promote seed germination.

[0057] (4) After the seeds germinate (4-7 days), adjust the temperature to 20℃, the humidity to 75%, and provide 12 hours of light per day.

[0058] Spray with neutral purified water with a pH of 6.5 every 2 days, at a rate of 200 ml per square meter. Harvest the sprouts after 7 days when they reach a height of 9 cm, and cut them off at the base with scissors.

[0059] Example 2

[0060] (1) Select the seeds of the Naiman strain of samosa, soak them in water for 8 hours to remove floating seeds, disinfect them with 0.1% sodium hypochlorite solution for 10 minutes, and then rinse them with water.

[0061] (2) Use a culture substrate made of coconut coir, perlite and vermiculite in a ratio of 3:1:1, fill it into a culture tray, sow the treated seeds on the substrate at a density of 5 seeds per square centimeter, cover with a thin gauze and compact it.

[0062] (3) In the early stage of cultivation (1-3 days), maintain a temperature of 25℃ and a humidity of 90%, and carry out shading treatment.

[0063] (4) After germination, the temperature is controlled at 25℃, the humidity at 70%, and 12 hours of light are provided every day.

[0064] Spray with neutral purified water with a pH of 7.0 every 2 days, at a rate of 300 ml per square meter. Harvest the sprouts when they grow to 10 cm in height after 8 days.

[0065] Example 3

[0066] (1) Select the seeds of the Ulanbuhe strain of sand rice, soak them in water for 7 hours, remove inferior seeds, disinfect them with 0.1% sodium hypochlorite solution for 10 minutes, and rinse them with water.

[0067] (2) The cultivation substrate is prepared by mixing coconut coir, perlite and vermiculite in a ratio of 3:1:1. The substrate is then placed in the cultivation tray, and the seed density is 4.5 seeds per square centimeter. The tray is then covered with a thin gauze and compacted.

[0068] (3) The initial temperature during cultivation is 23℃, the humidity is 88%, and the light is shading for 1-3 days.

[0069] (4) After germination, the temperature is 22℃, the humidity is 78%, and the light is 12 hours a day.

[0070] Spray with purified water with a pH of 6.8 every 2 days, 250 ml per square meter, and harvest after 7 days. The sprouts will grow to 8.5 cm tall.

[0071] Example 4

[0072] (1) Select high-quality sand rice seeds of the Erxiang strain, soak them in clean water for 8 hours, remove inferior seeds floating on the surface of the water during this period, then disinfect them with 0.1% sodium hypochlorite solution for 10 minutes, and then rinse them clean with water.

[0073] (2) Prepare the culture substrate by mixing coconut coir, perlite and vermiculite in a ratio of 3:1:1. Put the sterilized culture substrate into the culture tray, and then sow the treated seeds evenly on the culture substrate at a density of 4 seeds per square centimeter. Cover with a thin gauze of about 0.5 cm and press it down gently.

[0074] (3) In the early stage of cultivation (1-3 days), the ambient temperature is controlled at 22℃ and the humidity is kept at 85%. Shading treatment is carried out to promote seed germination.

[0075] (4) After the seeds germinate (4-7 days), adjust the temperature to 20℃, the humidity to 80%, and provide 12 hours of light per day.

[0076] Spray with neutral purified water with a pH of 6.5 every 2 days, at a rate of 200 ml per square meter. Harvest the sprouts after 7 days when they reach a height of 9 cm, and cut them off at the base with scissors.

[0077] Example 5

[0078] (1) Select high-quality sand rice seeds of the Haolaihure strain, soak them in clean water for 7 hours, remove inferior seeds floating on the surface of the water during this period, then disinfect them with 0.1% sodium hypochlorite solution for 10 minutes, and then rinse them clean with water.

[0079] (2) Prepare the culture substrate by mixing coconut coir, perlite and vermiculite in a ratio of 3:1:1. Put the sterilized culture substrate into the culture tray, and then sow the treated seeds evenly on the culture substrate at a density of 5 seeds per square centimeter. Cover with a thin gauze of about 0.5 cm and press it down gently.

[0080] (3) In the early stage of cultivation (1-3 days), the ambient temperature is controlled at 22℃ and the humidity is kept at 85%. Shading treatment is carried out to promote seed germination.

[0081] (4) After the seeds germinate (4-7 days), adjust the temperature to 20℃, the humidity to 75%, and provide 12 hours of light per day.

[0082] Spray with neutral purified water with a pH of 6.5 every 2 days, at a rate of 200 ml per square meter. Harvest the sprouts after 7 days when they reach a height of 9 cm, and cut them off at the base with scissors.

[0083] test

[0084] The content of γ-aminobutyric acid (GABA) in the sprouts obtained in Examples 1-5 was detected by the following methods:

[0085] Accurately weigh 0.1g each of the buds and seedlings from Examples 1-5, grind them thoroughly, add 1ml of 60% ethanol solution, and extract on a shaker at 40℃ for 2h. Centrifuge at 4000r / min for 10min, then take 0.5ml of the supernatant, add 0.2ml of 0.2mmol / L pH9.0 borate buffer and 1ml of 6% phenol solution, mix well, and let stand at room temperature for 5min. Then add 0.2ml of NaClO solution with an effective chlorine content of 5.6%, shake thoroughly, boil in a water bath for 10min, and then immediately in an ice water bath for 20min. After the solution turns blue-green, add 0.2ml of 60% ethanol solution, shake again to mix well, and let stand. Then measure the absorbance value at 645nm, and the standard curve is y = 1.8016x + 0.0052R² = 0.9992.

[0086] The test results are shown in Table 1.

[0087] Table 1. γ-aminobutyric acid (GABA) content (dw / g) in sprouts and seedlings of different local varieties of *Salix matsudana*.

[0088]

[0089] Note: DW: dry weight.

[0090] As shown in Table 1, the sprouts obtained by this invention have a high content of γ-aminobutyric acid (GABA).

[0091] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the specific implementation of the present invention. Any modifications or equivalent substitutions that do not depart from the spirit and scope of the present invention should be covered within the scope of protection of the claims of the present invention.

Claims

1. A method for cultivating *Solanum tuberosum* sprouts rich in γ-aminobutyric acid (GABA), characterized in that, Includes the following steps: Select sand rice seeds, soak them in clean water, and then disinfect them with sodium hypochlorite solution to obtain treated sand rice seeds; The treated sand rice seeds were sown in a culture medium, and then covered with a thin gauze for the first stage of cultivation. After the first stage of cultivation is completed, the second stage of cultivation is carried out. When the sprouts grow to 8-10 cm in height, they are harvested to obtain sand bonsai sprouts rich in γ-aminobutyric acid. The concentration of sand rice seeds during sowing is 4-5 seeds / cm³. 2 ; The conditions for the first stage of cultivation are: temperature 20-25℃, humidity 80%-90%, and shading. The conditions for the second stage of cultivation are: temperature of 15-25℃, humidity of 70%-80%, and sunlight exposure.

2. The method for cultivating *Solanum tuberosum* sprouts rich in γ-aminobutyric acid according to claim 1, characterized in that, The soaking time is 6 to 8 hours.

3. The method for cultivating *Solanum tuberosum* sprouts rich in γ-aminobutyric acid according to claim 1, characterized in that, The disinfection process involves disinfecting the seeds of *Salix matsudana* with a 0.1% sodium hypochlorite solution for 10 minutes, followed by rinsing them thoroughly with clean water.

4. The method for cultivating *Solanum tuberosum* sprouts rich in γ-aminobutyric acid according to claim 1, characterized in that, Both the first and second stages of cultivation involved spraying with neutral purified water, maintaining a pH between 6.5 and 7.

0. The spraying frequency was once every two days, with a spray volume of 200-300 mL / m³. 2 .

5. The method for cultivating *Solanum tuberosum* sprouts rich in γ-aminobutyric acid according to claim 1, characterized in that, The culture medium is composed of coconut coir, perlite and vermiculite in a 3:1:1 ratio.

6. The method for cultivating *Solanum tuberosum* sprouts rich in γ-aminobutyric acid according to claim 1, characterized in that, The selection of sand rice seeds specifically refers to selecting sand rice seeds that are plump and free from pests and diseases.

7. The application of the method for cultivating *Solanum tuberosum* sprouts rich in γ-aminobutyric acid as described in any one of claims 1 to 6 in the cultivation of *Solanum tuberosum* sprouts.

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