A fermented Chinese herbal medicine microecological preparation for preventing and treating sow mastitis, and a preparation method and application thereof

Abstract

The application discloses a kind of fermented Chinese herbal medicine microecological preparation for preventing and treating sow mastitis and a preparation method and application thereof, and belongs to the technical field of fermented traditional Chinese medicine preparation.The fermented Chinese herbal medicine microecological preparation for preventing and treating sow mastitis of the application is composed of pure traditional Chinese medicine and amino acid, and the microtoxicity substances in natural plant medicine are decomposed by microbial fermentation, the efficacy is enhanced, the safety is high, and the preparation is added in sow feed at 1-3%, which can effectively prevent the occurrence of sow mastitis and has a certain therapeutic effect on sow mastitis.The fermented Chinese herbal medicine microecological preparation provided by the application has no acute and chronic toxicity, is a safe preparation, can significantly reduce the incidence of sow mastitis or promote the recovery of sow mastitis, is an ideal functional preparation for preventing and treating sow mastitis, and has good application prospect.

Description

Technical Field

[0001] This invention relates to the field of fermented traditional Chinese medicine preparation technology, and in particular to a fermented traditional Chinese herbal microecological preparation for the prevention and treatment of mastitis in sows, its preparation method and application. Background Technology

[0002] Mastitis in sows is a relatively common reproductive disorder with a high incidence rate and is difficult to prevent and treat. After developing mastitis, sows experience elevated body temperature, decreased appetite, a significant drop in milk production, and pathological changes in milk composition, which harms the growth and development of piglets and can even lead to the death of the entire litter. If mastitis is not treated promptly and effectively, it often progresses from acute to chronic, or becomes subclinical or purulent mastitis, leading to premature culling of sows, causing significant economic losses to farmers, and seriously hindering the healthy development of the pig farming industry.

[0003] Traditional Chinese medicine (TCM) herbs are rich in protein, vitamins, and minerals, as well as bioactive substances such as alkaloids, glycosides, and sterols, which can enhance immunity and reduce inflammation. Microbial fermentation is a traditional processing method for TCM herbs. After fermentation with probiotics, the utilization rate of active ingredients is improved, the efficacy is enhanced, and the toxicity is reduced. It also offers advantages such as improved immunity, low residue, and green safety, making it a promising candidate for application in animal husbandry. However, there are currently no reports of using amino acids in conjunction with fermented TCM herbs to prevent mastitis in sows. Summary of the Invention

[0004] The purpose of this invention is to provide a fermented traditional Chinese medicine microecological preparation for the prevention and treatment of sow mastitis, its preparation method and application, in order to solve the problems existing in the prior art. The fermented traditional Chinese medicine microecological preparation provided by this invention can effectively prevent the occurrence of sow mastitis and play a certain therapeutic role in sow mastitis.

[0005] To achieve the above objectives, the present invention provides the following solution: This invention provides a fermented traditional Chinese medicine microecological preparation for the prevention and treatment of mastitis in sows, which is prepared by fermentation of traditional Chinese medicine and amino acid components through compound microorganisms; By weight, the herbal medicines and amino acid components include: honeysuckle 30-90 parts, forsythia 20-60 parts, rehmannia glutinosa 25-75 parts, scrophularia ningpoensis 25-75 parts, dandelion 20-60 parts, viola yedoensis 20-60 parts, angelica sinensis 15-45 parts, frankincense 10-40 parts, myrrh 10-40 parts, vaccaria segetalis 15-45 parts, licorice 10-30 parts, echinops latifolius 15-45 parts, and 5-hydroxytryptophan 100-200 parts; The composite microorganisms include Bacillus subtilis, Clostridium truncatum, Lactobacillus bulgaricus, and Enterococcus faecalis.

[0006] Optionally, the Bacillus subtilis, Clostridium truncatum, Lactobacillus bulgaricus and Enterococcus faecalis are mixed in the form of bacterial solution at a volume ratio of 1:(1-3):(0.5-1):(2-3); The bacterial concentrations of Bacillus subtilis, Clostridium tenuifolium, Lactobacillus bulgaricus, and Enterococcus faecalis were all 1×10⁻⁶. 9 -1×10 10 CFU / mL.

[0007] Preferably, the Bacillus subtilis, Clostridium tenuifolium, Lactobacillus bulgaricus, and Enterococcus faecalis are in a bacterial solution with a volume ratio of 1:2:0.5:2.

[0008] Preferably, by weight, the herbal and amino acid components include: 60 parts honeysuckle, 40 parts forsythia, 50 parts rehmannia root, 50 parts scrophularia root, 40 parts dandelion, 40 parts violet, 30 parts angelica, 20 parts frankincense, 20 parts myrrh, 30 parts vaccaria seed, 20 parts licorice, 30 parts elm bark, and 150 parts 5-hydroxytryptophan.

[0009] This invention provides a method for preparing the fermented traditional Chinese medicine microecological preparation, comprising the following steps: Bacillus subtilis, Clostridium tenuifolium, Lactobacillus bulgaricus, and Enterococcus faecalis were mixed in bacterial solution at a volume ratio of 1:(1-3):(0.5-1):(2-3) to obtain a composite microorganism. After drying and pulverizing honeysuckle, forsythia, rehmannia, scrophularia, dandelion, viola, angelica, frankincense, myrrh, vaccaria, licorice, and elm, the mixture is passed through a 60-80 mesh sieve and mixed with 5-hydroxytryptophan to obtain traditional Chinese medicine and amino acid components. Then, compound microorganisms are added for fermentation, drying, and micro-pulverization to obtain the final product.

[0010] Optionally, the mass ratio of the traditional Chinese medicine and amino acid components to the compound microorganisms is 20:(1-2). Optionally, the bacterial concentrations of the Bacillus subtilis, Clostridium tenuifolium, Lactobacillus bulgaricus, and Enterococcus faecalis bacterial solutions are all 1×10⁻⁶. 9 -1×10 10 CFU / mL.

[0011] Optionally, the fermentation conditions are 30-35℃ for 36-48 hours.

[0012] This invention provides the application of the fermented herbal microecological preparation in the preparation of drugs for the prevention and / or treatment of mastitis in sows.

[0013] This invention provides a product for the prevention and / or treatment of mastitis in sows, comprising the aforementioned fermented herbal microecological preparation.

[0014] The present invention discloses the following technical effects: The fermented herbal microecological preparation for preventing and treating sow mastitis of the present invention is composed of pure Chinese medicine and amino acids. It decomposes the slightly toxic substances in natural plant medicines through microbial fermentation, enhances the efficacy, and has a high safety. When added to sow feed at 1% to 3%, it can effectively prevent the occurrence of sow mastitis and play a certain therapeutic role in sow mastitis.

[0015] To examine the safety and efficacy of this product, an experiment lasting more than three months was conducted. The results showed that the fermented herbal microecological preparation provided by this invention has no acute or chronic toxicity and is a safe preparation. It can significantly reduce the incidence of mastitis in sows or promote the cure of mastitis in sows. It is an ideal functional preparation for the prevention and treatment of mastitis in sows and has good application prospects. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 To investigate the effects of fermented traditional Chinese medicine microecological preparations on the incidence of mastitis in sows, average daily feed intake during lactation, weaning litter weight, IgA, IgG, and IgM; Figure 2 The effects of fermented traditional Chinese medicine microecological preparations on the cure rate, TNF-α, IL-1β and IL-6 in sows with mastitis. Detailed Implementation

[0018] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0019] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any stated value or intermediate value within a stated range, as well as each smaller range between any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0020] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0021] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0022] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0023] Honeysuckle: It is sweet and cold in nature, and enters the lung, heart and stomach meridians. It has the effects of clearing heat and detoxifying, and cooling and dispersing wind-heat. It is mainly used to clear heat and detoxify, and cool and disperse wind-heat. Forsythia: It is bitter and slightly cold in nature, and enters the lung, heart and small intestine meridians. It has the effects of clearing heat and detoxifying, reducing swelling and dissipating nodules, and dispersing wind-heat. It is mainly used to treat carbuncles, scrofula, mastitis, erysipelas, wind-heat cold, the initial stage of febrile disease, high fever and thirst, and painful urination. Rehmannia glutinosa: It is sweet, bitter, and cold in nature, and enters the heart, liver, and kidney meridians. It has the effects of clearing heat and cooling blood, nourishing yin and promoting body fluids. It is mainly used to treat heat entering the blood, fever due to yin deficiency, thirst due to fluid depletion, internal heat and thirst, bleeding due to blood heat, and constipation due to intestinal dryness. Scrophularia: It is bitter, sweet, salty, and cold in nature. It enters the lung, stomach, and kidney meridians. It has the effects of nourishing yin and reducing fire, detoxifying and dispersing nodules. It is mainly used to treat febrile diseases with heat entering the blood level, sore throat, rashes, carbuncles and boils, scrofula and phlegm nodules. Dandelion: It is sweet, bitter, and cold in nature, and enters the liver and stomach meridians. It has the effects of clearing heat and detoxifying, reducing swelling and dissipating nodules, and promoting diuresis and relieving strangury. It is mainly used to treat carbuncles, boils, mastitis, jaundice due to heat strangury, and red, swollen and painful eyes. Viola yedoensis: It is bitter, pungent, and cold in nature, and enters the heart and liver meridians. It has the effects of clearing heat and detoxifying, cooling blood and reducing swelling. It is mainly used to treat boils, carbuncles, erysipelas, and snake bites. Angelica sinensis: sweet, pungent, and warm in nature, enters the liver, heart, and spleen meridians. It has the effects of nourishing blood and promoting blood circulation, regulating menstruation and relieving pain, and moistening the intestines and relieving constipation. It is mainly used to treat blood deficiency and chlorosis, irregular menstruation, dysmenorrhea, traumatic injuries, and constipation due to intestinal dryness. Frankincense: It is pungent, bitter, and warm in nature, and enters the heart, liver, and spleen meridians. It has the effects of promoting blood circulation, relieving pain, reducing swelling, and promoting tissue regeneration. It is mainly used to treat traumatic injuries, carbuncles and sores, dysmenorrhea, and various pains in the heart and abdomen. Myrrh: It is bitter, pungent, and neutral in nature. It enters the heart, liver, and spleen meridians. It has the effects of dispersing blood stasis and relieving pain, reducing swelling and promoting tissue regeneration. It is mainly used to treat traumatic injuries, non-healing sores, dysmenorrhea, and abdominal masses. Wang Bu Liu: It is bitter and neutral in nature, and enters the liver and stomach meridians. It has the effects of promoting blood circulation and regulating menstruation, promoting lactation and reducing swelling. It is mainly used to treat amenorrhea, dysmenorrhea, insufficient lactation, and mastitis. Licorice: It is sweet and neutral in nature, and enters the heart, lung, spleen and stomach meridians. It has the effects of tonifying the spleen and replenishing qi, clearing heat and detoxifying, and harmonizing the properties of other medicines. It is mainly used to treat spleen and stomach weakness, cough with phlegm, carbuncles and boils, and harmonize the properties of other medicines. Loulu: It is bitter, salty, and cold in nature, and enters the stomach and large intestine meridians. It has the effects of clearing heat and detoxifying, eliminating carbuncles and dissipating nodules, and promoting menstruation and lactation. It is mainly used to treat mastitis, scrofula, and insufficient lactation.

[0024] The core principle of this traditional Chinese medicine formula for treating mastitis in sows is to primarily clear heat and detoxify, eliminate carbuncles and dissipate nodules, while secondarily promoting blood circulation, clearing the meridians, reducing swelling and relieving pain, and simultaneously nourishing yin and preventing damage to the body's vital energy. Targeting the core pathological aspect of mastitis—milk stasis—honeysuckle and forsythia are used as the principal herbs to powerfully clear heat and toxins, and dissipate carbuncles and nodules; dandelion, violet, lophatherum, rehmannia, and scrophularia are used as the assistant herbs to target clearing blood heat, nourishing yin and fluids, eliminating mastitis, and promoting lactation; angelica, frankincense, myrrh, and vaccaria are used as adjuvant herbs to relieve pain and swelling, promote blood circulation, clear the meridians, and relieve pain in the mammary ducts; and licorice is used as the guiding herb to harmonize the other herbs and protect the stomach.

[0025] The Bacillus subtilis, Clostridium truncatum, Lactobacillus bulgaricus, and Enterococcus faecalis used in this invention were all provided by Shandong Taishan Shengluyuan Group Co., Ltd.

[0026] Example 1: A fermented traditional Chinese medicine microecological preparation for preventing and treating mastitis in sows and its preparation method. By weight, the fermented herbal microecological preparation contains the following herbal and amino acid components: 60 parts honeysuckle, 40 parts forsythia, 50 parts rehmannia root, 50 parts scrophularia root, 40 parts dandelion, 40 parts violet, 30 parts angelica root, 20 parts frankincense, 20 parts myrrh, 30 parts vaccaria seed, 20 parts licorice root, 30 parts echinops, and 15 parts 5-hydroxytryptophan; and compound microecological preparation components: Bacillus subtilis, Clostridium tenuifolium, Lactobacillus bulgaricus, and Enterococcus faecalis, each mixed in bacterial solution with a concentration of 1×10⁻⁶. 9 CFU / mL, with a volume ratio of 1:2:0.5:2.

[0027] Preparation method: A compound microbial preparation was prepared by mixing Bacillus subtilis, Clostridium perfringens, Lactobacillus bulgaricus, and Enterococcus faecalis in a volume ratio of 1:2:0.5:2. Honeysuckle, Forsythia suspensa, Rehmannia glutinosa, Scrophularia ningpoensis, Taraxacum mongolicum, Viola yedoensis, Angelica sinensis, Boswellia carterii, Commiphora myrrha, Vaccaria segetalis, Glycyrrhiza uralensis, and Echinops latifolius according to the specified proportions, then washing, drying, and pulverizing them. The pulverized herbs were passed through a 60-mesh sieve and mixed with 5-hydroxytryptophan to obtain the herbal medicine and amino acid components. Then, 35 parts of the compound microbial preparation were added and fermented at 32℃ for 48 h. After fermentation, the mixture was dried at 55℃ for 12 h, then micronized and passed through a 300-mesh sieve to obtain the fermented herbal medicine microecological preparation.

[0028] Example 2: A fermented traditional Chinese medicine microecological preparation for preventing and treating mastitis in sows and its preparation method. By weight, the fermented herbal microecological preparation contains the following herbal and amino acid components: 90 parts honeysuckle, 30 parts forsythia, 25 parts rehmannia root, 75 parts scrophularia root, 30 parts dandelion, 60 parts violet, 15 parts angelica root, 40 parts frankincense, 10 parts myrrh, 20 parts vaccaria seed, 30 parts licorice root, 25 parts echinops latifolius, and 180 parts 5-hydroxytryptophan; and compound microecological preparation components: Bacillus subtilis, Clostridium tenuifolium, Lactobacillus bulgaricus, and Enterococcus faecalis, all mixed in bacterial solution form, with a bacterial concentration of 1.5 × 10⁻⁶. 9 CFU / mL, with a volume ratio of 1:3:1:2.5.

[0029] Preparation method: A compound microbial preparation was prepared by mixing Bacillus subtilis, Clostridium perfringens, Lactobacillus bulgaricus, and Enterococcus faecalis in a volume ratio of 1:3:1:2.5. Honeysuckle, Forsythia suspensa, Rehmannia glutinosa, Scrophularia ningpoensis, Taraxacum mongolicum, Viola yedoensis, Angelica sinensis, Boswellia carterii, Commiphora myrrha, Vaccaria segetalis, Glycyrrhiza uralensis, and Echinops latifolius according to the specified proportions, then washing, drying, and pulverizing them. The pulverized herbs were passed through an 80-mesh sieve and mixed with 5-hydroxytryptophan to obtain the herbal medicine and amino acid components. Then, 50 parts of the compound microbial preparation were added and fermented at 33℃ for 40 h. After fermentation, the mixture was dried at 57℃ for 10 h, then micronized and passed through a 300-mesh sieve to obtain the fermented herbal medicine microecological preparation.

[0030] Example 3: A fermented traditional Chinese medicine microecological preparation for preventing and treating mastitis in sows and its preparation method. By weight, the fermented herbal microecological preparation contains the following herbal and amino acid components: honeysuckle 30 parts, forsythia 60 parts, rehmannia 75 parts, scrophularia 25 parts, dandelion 60 parts, violet 30 parts, angelica 45 parts, frankincense 10 parts, myrrh 40 parts, vaccaria segetalis 15 parts, licorice 10 parts, echinops latifolius 15 parts, and 5-hydroxytryptophan 100 parts; and compound microecological preparation components: Bacillus subtilis, Clostridium tenuifolium, Lactobacillus bulgaricus, and Enterococcus faecalis, each mixed in bacterial solution, with a bacterial concentration of 1×10⁻⁶. 9 CFU / mL, with a volume ratio of 1:1:0.5:2.

[0031] Preparation method: A compound microbial preparation was prepared by mixing Bacillus subtilis, Clostridium tenuifolium, Lactobacillus bulgaricus, and Enterococcus faecalis in a volume ratio of 1:1:0.5:2. Honeysuckle, Forsythia suspensa, Rehmannia glutinosa, Scrophularia ningpoensis, Taraxacum mongolicum, Viola yedoensis, Angelica sinensis, Boswellia carterii, Commiphora myrrha, Vaccaria segetalis, Glycyrrhiza uralensis, and Echinops latifolius according to the specified proportions, then washing, drying, and pulverizing them. The pulverized herbs were passed through a 70-mesh sieve and mixed with 5-hydroxytryptophan to obtain the herbal medicine and amino acid components. Then, 36 parts of the compound microbial preparation were added and fermented at 35℃ for 36 h. After fermentation, the mixture was dried at 60℃ for 8 h, then micronized and passed through a 300-mesh sieve to obtain the fermented herbal microecological preparation.

[0032] Example 4: A fermented traditional Chinese medicine microecological preparation for preventing and treating mastitis in sows and its preparation method. By weight, the fermented herbal microecological preparation contains the following herbal and amino acid components: honeysuckle 45 parts, forsythia 20 parts, rehmannia 60 parts, scrophularia 30 parts, dandelion 20 parts, violet 20 parts, angelica 20 parts, frankincense 30 parts, myrrh 30 parts, vaccaria 45 parts, licorice 25 parts, echinops 45 parts, and 5-hydroxytryptophan 150 parts; and compound microecological preparation components: Bacillus subtilis, Clostridium truncatum, Lactobacillus bulgaricus, and Enterococcus faecalis, each mixed in bacterial solution, with a bacterial concentration of 1×10⁻⁶. 10 CFU / mL, with a volume ratio of 1:2.5:1:3.

[0033] Preparation method: A compound microbial preparation was prepared by mixing Bacillus subtilis, Clostridium perfringens, Lactobacillus bulgaricus, and Enterococcus faecalis in a volume ratio of 1:2.5:1:3. Honeysuckle, Forsythia suspensa, Rehmannia glutinosa, Scrophularia ningpoensis, Taraxacum mongolicum, Viola yedoensis, Angelica sinensis, Boswellia carterii, Commiphora myrrha, Vaccaria segetalis, Glycyrrhiza uralensis, and Echinops latifolius according to the specified proportions, then washing, drying, and pulverizing them. The pulverized herbs were passed through a 60-mesh sieve and mixed with 5-hydroxytryptophan to obtain the herbal medicine and amino acid components. Then, 50 parts of the compound microbial preparation were added and fermented at 30℃ for 48 h. After fermentation, the mixture was dried at 58℃ for 10 h, then micronized and passed through a 300-mesh sieve to obtain the fermented herbal medicine microecological preparation.

[0034] Application Example 1: 0.2kg-100kg, 0.002kg-1kg, 2g-1kg A feeding trial for pregnant sows was conducted at a large pig farm in Dongying, Shandong Province. One thousand hundred healthy Landrace sows in their second to fourth parity were randomly divided into a control group and an experimental group, with 500 sows in each group. The control group was fed a normal commercial diet (purchased from Shandong Bangji Technology Co., Ltd.), while the experimental group received the fermented herbal microecological preparation prepared in Example 1 of this invention at a ratio of 1% added to their commercial diet. The trial period lasted from 90 days of gestation to 21 days of lactation, ending at weaning. Daily feed intake and litter weight at weaning were recorded during the trial. The concentrations of IgA, IgG, and IgM in the plasma of sows on the day of farrowing were measured using an ELISA kit (purchased from Beijing Solarbio Science & Technology Co., Ltd.), and the incidence of mastitis in sows was also recorded.

[0035] turn out( Figure 1 Compared with the control group, the experimental group showed a 18.8% decrease in mastitis incidence throughout the experimental period, an increase of 0.83 kg / day in average daily feed intake during lactation, and an increase of 8.25 kg / litter weight in weaned piglets. Furthermore, the plasma concentrations of IgA, IgG, and IgM in the experimental group on the day of farrowing were significantly higher than those in the control group (45.03, 482.47, and 39.78 μg / mL, respectively) (35.25, 422.50, and 32.25 μg / mL, respectively). These results indicate that the fermented herbal microecological preparation provided by this invention can effectively enhance the immune function of sows, reduce the incidence of mastitis, and improve their lactation performance.

[0036] Application Example 2 A feeding trial was conducted on sows at a large pig farm in Dongying, Shandong Province. Fifty lactating sows with varying degrees of mastitis were randomly divided into two treatments. The control group was fed a normal commercial diet (purchased from Shandong Bangji Technology Co., Ltd.), while the experimental group received the fermented herbal microecological preparation prepared in Example 1 of this invention at a ratio of 3% added to the commercial diet. The trial lasted for 7 days. Other treatments for mastitis in sows during the trial were performed according to the farm's standard procedures. Colostrum and normal milk were collected from each group of sows, and the concentrations of inflammatory factors TNF-α, IL-1β, and IL-6 were measured using an ELISA kit (purchased from Beijing Solarbio Science & Technology Co., Ltd.). The cure rate of mastitis in each group was calculated after the trial.

[0037] turn out( Figure 2Compared with the control group, the cure rate of mastitis in the experimental group was increased by 72%. The concentrations of inflammatory factors TNF-α (59.0 and 62.1 ng / L), IL-1β (17.7 and 15.3 ng / L), and IL-6 (722.5 and 745.0 ng / L) in the colostrum and normal milk of the experimental group were significantly lower than those in the control group (TNF-α (66.5 and 88.3 ng / L), IL-1β (25.5 and 27.8 ng / L), and IL-6 (870.0 and 909.2 ng / L). These results indicate that the fermented herbal microecological preparation provided by this invention can have a therapeutic effect on mastitis in sows.

[0038] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A fermented traditional Chinese medicine microecological preparation for the prevention and treatment of mastitis in sows, characterized in that, It is prepared by fermentation of traditional Chinese medicine and amino acid components using compound microorganisms. The herbal medicines and amino acid components, by weight, are: honeysuckle 30-90 parts, forsythia 20-60 parts, rehmannia glutinosa 25-75 parts, scrophularia ningpoensis 25-75 parts, dandelion 20-60 parts, viola yedoensis 20-60 parts, angelica sinensis 15-45 parts, frankincense 10-40 parts, myrrh 10-40 parts, vaccaria segetalis 15-45 parts, licorice 10-30 parts, echinops latifolius 15-45 parts, and 5-hydroxytryptophan 100-200 parts; The composite microorganisms are Bacillus subtilis, Clostridium capillaris, Lactobacillus bulgaricus, and Enterococcus faecalis; The preparation method of the fermented herbal microecological preparation includes the following steps: Bacillus subtilis, Clostridium tenuifolium, Lactobacillus bulgaricus, and Enterococcus faecalis were mixed in bacterial suspension at a volume ratio of 1:(1-3):(0.5-1):(2-3) to obtain a composite microorganism; the bacterial concentrations of Bacillus subtilis, Clostridium tenuifolium, Lactobacillus bulgaricus, and Enterococcus faecalis were all 1×10⁻⁶. 9 -1×10 10 CFU / mL; After drying and pulverizing honeysuckle, forsythia, rehmannia, scrophularia, dandelion, viola, angelica, frankincense, myrrh, vaccaria, licorice, and elm, the mixture is passed through a 60-80 mesh sieve and mixed with 5-hydroxytryptophan to obtain traditional Chinese medicine and amino acid components. Then, compound microorganisms are added for fermentation, drying, and micro-pulverization to obtain the final product. The weight ratio of the traditional Chinese medicine and amino acid components to the composite microorganisms is 20:(1-2). The fermentation conditions are 30-35℃ for 36-48 hours.

2. The fermented herbal microecological preparation as described in claim 1, characterized in that, The Bacillus subtilis, Clostridium tenuifolium, Lactobacillus bulgaricus, and Enterococcus faecalis were mixed in a bacterial solution at a volume ratio of 1:2:0.5:

2.

3. The fermented herbal microecological preparation as described in claim 1, characterized in that, By weight, the herbal medicines and amino acid components include: 60 parts honeysuckle, 40 parts forsythia, 50 parts rehmannia root, 50 parts scrophularia root, 40 parts dandelion, 40 parts violet, 30 parts angelica, 20 parts frankincense, 20 parts myrrh, 30 parts vaccaria seed, 20 parts licorice, 30 parts elm bark, and 150 parts 5-hydroxytryptophan.

4. The method for preparing fermented herbal microecological preparations as described in claim 1, characterized in that, Includes the following steps: Bacillus subtilis, Clostridium tenuifolium, Lactobacillus bulgaricus, and Enterococcus faecalis were mixed in a bacterial solution at a volume ratio of 1:(1-3):(0.5-1):(2-3) to obtain a composite microorganism. After drying and pulverizing honeysuckle, forsythia, rehmannia root, scrophularia root, dandelion, viola, angelica, frankincense, myrrh, vaccaria seed, licorice, and elm, the mixture is passed through a 60-80 mesh sieve and mixed with 5-hydroxytryptophan to obtain traditional Chinese medicine and amino acid components. Then, compound microorganisms are added for fermentation, drying, and micro-pulverization to obtain the final product.

5. The preparation method according to claim 4, characterized in that, The weight ratio of the herbal medicine and amino acid components to the compound microorganisms is 20:(1-2).

6. The preparation method according to claim 4, characterized in that, The bacterial concentrations of the Bacillus subtilis, Clostridium tenuifolium, Lactobacillus bulgaricus, and Enterococcus faecalis bacterial suspensions were all 1×10⁻⁶. 9 -1×10 10 CFU / mL.

7. The preparation method according to claim 4, characterized in that, The fermentation conditions are 30-35℃ for 36-48 hours.

8. The use of the fermented herbal microecological preparation as described in claim 1 in the preparation of drugs for the prevention and / or treatment of mastitis in sows.

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