一种热稳定性提高的UDP-糖焦磷酸化酶及其编码基因和应用
By designing mutant UDP-glucose pyrophosphorylase with a specific amino acid sequence, the problem of poor thermostability of UDP-glucose pyrophosphorylase was solved, achieving higher thermostability and enzyme activity, which promoted the synthesis of UDP-Gal and the production of human milk oligosaccharides.
CN120796230BActive Publication Date: 2026-07-17HEBEI UNIV OF SCI & TECH
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- HEBEI UNIV OF SCI & TECH
- Filing Date
- 2025-06-06
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
The poor thermal stability of existing UDP-glucose pyrophosphorylase (AtUSP) limits the synthesis of UDP-Gal and the widespread application of human milk oligosaccharides, and increases production costs.
Method used
The thermostability and enzyme activity of UDP-glucose pyrophosphorylases were improved by designing and expressing mutant UDP-glucose pyrophosphorylases with specific amino acid sequences, such as K561Y, L492F, S99T, N107F, S545T, M1, M2, M3 and M4.
Benefits of technology
The optimal reaction temperature of the mutant enzyme was increased to 55℃, and the half-life was increased to 10.615 hours, which is 53.08 times that of the wild type, significantly improving the enzyme's thermal stability and production efficiency.
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Figure CN120796230B_ABST
Abstract
本发明属于基因工程技术领域，具体地，涉及一种热稳定性提高的UDP‑糖焦磷酸化酶及其编码基因和应用。以野生型UDP‑糖焦磷酸化酶基因序列为模板，进行定点突变，得到了热稳定性显著提高的UDP‑糖焦磷酸化酶，可以应用于UDP‑Gal合成，具有重要价值。
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