一种紫花苜蓿自交不亲和相关蛋白及其编码基因与应用

By regulating the S-RNase-77 protein and its encoding gene in alfalfa, the problem of self-incompatibility limiting breeding in alfalfa was solved, and plants with altered self-incompatibility were cultivated, which improved the pod setting rate and seed number, thus promoting the precision and efficiency of breeding.

CN120796236BActive Publication Date: 2026-07-17INST OF BOTANY CHINESE ACAD OF SCI +3

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF BOTANY CHINESE ACAD OF SCI
Filing Date
2025-07-24
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Self-incompatibility of alfalfa has limited its breeding progress, and existing research has not yet clarified its molecular mechanism, which affects the accuracy and efficiency of breeding.

Method used

An S-RNase-77 protein with the amino acid sequence SEQ ID No:1 and its encoding gene are provided. Plant self-incompatibility can be regulated by genetic engineering methods, including constructing a recombinant vector and introducing it into plant cells, to inhibit or enhance the expression of S-RNase-77 to change self-incompatibility.

Benefits of technology

We successfully bred alfalfa plants with altered self-incompatibility, increasing pod setting rate and seed number, providing a foundation for breeding new varieties, and elucidating the molecular mechanism of alfalfa self-incompatibility.

✦ Generated by Eureka AI based on patent content.

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Abstract

本发明公开了一种紫花苜蓿自交不亲和相关蛋白及其编码基因与应用。本发明属于生物技术领域,具体涉及一种紫花苜蓿自交不亲和相关蛋白及其编码基因与应用。本发明的蛋白质是如下任一种的蛋白质:A1)氨基酸序列如SEQ ID No:1所示的蛋白质;A2)将A1)的蛋白质经过氨基酸残基的取代和 / 或缺失和 / 或添加得到的与A1)所示的蛋白质具有80%以上的同一性且具有相同功能的蛋白质;A3)在A1)或A2)的N末端或 / 和C末端连接蛋白质标签得到的融合蛋白质。与受体材料相比,利用干扰载体将S‑RNase‑77的靶标片段导入中苜1号,得到的转基因植株单株部分自交结实,结荚数提高,种子数增加。
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