Environment-friendly automatic implementation of gene editing system without transgenic elements and application thereof

By introducing toxic elements driven by promoters specific to transgenic male and female gametes into plants through the pTGAC system, the problem of transgenic pollen escape was solved, enabling autonomous isolation of transgenic plants and environmentally friendly gene editing.

CN120796337BActive Publication Date: 2026-06-19WUHAN UNIV
4 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
WUHAN UNIV
Filing Date
2024-10-30
Publication Date
2026-06-19

AI Technical Summary

⚠Technical Problem

Existing technologies are insufficient to effectively prevent environmental pollution caused by the escape of genetically modified pollen, especially since it is difficult to isolate genetically modified elements in polyploid plants, and traditional methods have failed to completely remove genetically modified pollen.

⚗Method used

The pTGAC system was used to kill transgenic male and female gametes by introducing toxic elements driven by promoters specific to transgenic male and female gametes into plants, ensuring that no transgenic gametes participated in fertilization and obtaining transgenic-free plants.

🎯Benefits of technology

It achieves autonomous isolation of non-GMO plants, avoiding pollen escape and exogenous gene contamination, and is particularly suitable for polyploid plants, making it environmentally friendly.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
Patent Text Reader

Abstract

This invention discloses an environmentally friendly, automated gene editing system for achieving transgenic element-free selection and its applications, belonging to the field of genetic engineering technology. The gene editing system of this invention comprises a gene editing vector containing transgenic male gamete-killing elements and transgenic female gamete-killing elements. The transgenic male gamete-killing element contains the AtDUO1 promoter and a modified Barnase gene; the transgenic female gamete-killing element contains the AtDD22 promoter and a modified Barnase gene. When transfected into plants, this gene editing system can kill transgenic male and female gametes, enabling transgenic-free male and female gametes to survive and participate in fertilization, resulting in transgenic-free plants. The selection of transgenic-free gametes in this gene editing system is automatic, eliminating the problems of pollen escape and exogenous gene pollution, making it an environmentally friendly gene editing system.
Need to check novelty before this filing date? Find Prior Art