Alcohol-precipitable soluble lotus root dietary fiber preparation method and product thereof

Abstract

The invention relates to a preparation method of a lotus root residue extract, which comprises the following steps: S1, gelatinizing lotus root residues at 65-95 DEG C for 5-30 minutes, then adding amylase and saccharifying enzyme, adding the amylase according to 32-3,000U / kg of the lotus root residues, and adding the saccharifying enzyme according to 14-13,000U / kg of the lotus root residues; s2, raising the temperature to be not lower than 80 DEG C until the enzyme is inactivated; s3, cooling to 50-60 DEG C, and then adding lysozyme of 400,000-12,000,000 U / kg of the lotus root residues and / or cellulase of 5,000,000-2,000,000 U / kg of the lotus root residues; and S4, adding ethanol to dissolve soluble components, and drying. According to the method, a specific enzymolysis process is innovatively adopted, so that high-valued utilization of lotus root residues as a lotus root processing byproduct is successfully realized, and the method has unique advantages in the aspects of remarkably improving the yield and functional characteristics of SDFP (alcohol-precipitable soluble dietary fiber polysaccharide). According to the process, a graded enzymolysis strategy is adopted, and in the first stage, the synergistic effect of amylase and saccharifying enzyme is utilized, so that a starch matrix in the lotus root residues is efficiently degraded, and SDFP precursor molecules are preliminarily released through specific binding of enzyme active sites and a substrate;

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