CpG site combinations, methods, and systems for cross-tissue individual age inference

Abstract

The invention discloses a CpG site combination, method and system for cross-tissue individual age inference, and belongs to the technical field of forensic medicine age inference, and the CpG site combination comprises chr6: 11044628, chr1: 207823723, chr7: 130734355, chr3: 160450189, chr19: 18233127, chr6: 25652377, chr2: 105399282, chr7: 33895826, chr1: 236394370 and chr3: 9552580; according to the present invention, by detecting the methylation level of the CpG site combination in the blood, the saliva, the oral swab or the mixture thereof derived from the individual biological sample, and combining with a specific age inference model, the high-precision age inference can be achieved. The CpG site combination and the method show low error and high robustness in cross-tissue application, have good tolerance to low initial DNA amount and common storage conditions, verify application potential in simulated forensic physical evidence, and provide important biomarker combination and technical scheme for forensic age inference.

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