MOF loaded copper single atom SERS biosensor and application thereof in detection of A beta1-42

Abstract

The invention belongs to the technical field of biological detection, and relates to an SERS (Surface Enhanced Raman Scattering) biosensor with MOF (Metal Organic Framework) loaded copper single atoms and application of the SERS biosensor in detection of Abeta1-42. According to the application of the SERS biosensor in detection of A beta 1-42, the SERS biosensor comprises a substrate and a Cu1 / MOF-808 layer located on the substrate, and the Cu1 / MOF-808 layer is formed by loading Cu single atoms on MOF-808. The detection limit of the Cu1 / MOF-808 sensor prepared by the invention on A beta 1-42 is as low as 10 <-13 > mol / L, the Cu1 / MOF-808 sensor has ultra-sensitive detection capability on A beta 1-42, the Raman characteristic peak intensity presented when the Cu1 / MOF-808 sensor detects the A beta 1-42 is in strong linear correlation with the concentration, and quantitative detection of the A beta 1-42 can be accurately realized; the Raman spectrum of A beta 1-42 and A beta 1-40 and the Raman spectrum of hemoglobin can be distinguished, the average accuracy rate of ten-time five-fold cross validation is 95.6%, and 100% classification accuracy rate is achieved through confusion matrix analysis.

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