Duck nmbR polypeptide, polyclonal antibody and application thereof

By preparing duck NMBR peptides and conjugating them with carrier proteins to obtain a complete antigen, immunizing animals and detecting the antiserum titer, the prepared polyclonal antibody can specifically bind to NMBR protein in duck tissues, solving the problem of the lack of duck NMBR protein antibodies on the market, and realizing low-cost, high-titer duck NMBR protein detection.

CN120829495BActive Publication Date: 2026-07-21JIANGSU INST OF POULTRY SCI +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGSU INST OF POULTRY SCI
Filing Date
2025-06-27
Publication Date
2026-07-21

AI Technical Summary

Technical Problem

There is a lack of antibodies on the market for detecting duck NMBR protein. The preparation of duck NMBR monoclonal antibodies is a complex, costly, and time-consuming process, which has led to research on NMBR in ducks mainly focusing on the gene level, severely restricting its functional research.

Method used

Duck NMBR polypeptide was prepared and coupled with a carrier protein to obtain a complete antigen, which was then used to immunize animals. The titer of the antiserum was detected by indirect ELISA. Polyclonal antibodies were then prepared to specifically bind to NMBR protein in duck tissues.

Benefits of technology

The prepared polyclonal antibody can specifically bind to NMBR protein in duck tissues, filling the gap in the research on the detection of duck NMBR protein and laying the foundation for the study of NMBR function in ducks. The method is simple, low-cost and highly effective.

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Abstract

The application discloses a duck NMBR polypeptide, a polyclonal antibody and application, and belongs to the field of biochemistry and molecular immunology. The duck NMBR gene is cloned for the first time, and the structure is analyzed; the amino acid at the 341-359 site of the carboxylic end of the duck NMBR protein is used as a hapten, coupled with keyhole limpet hemocyanin to prepare a complete antigen, then animals are immunized, and a polyclonal antibody of the duck NMBR polypeptide is separated and purified from antiserum. It is found through experiments that the polyclonal antibody can specifically combine with the NMBR protein in duck tissues. The application fills the blank in the field of duck NMBR protein detection research, and lays a foundation for the research on the function of NMBR in the duck body.
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