一种鸡FSHR多克隆抗体、制备方法及应用
By preparing polyclonal antibodies against chicken FSHR, the problem of a lack of antibodies for detecting chicken FSHR protein in the market has been solved. This has enabled the preparation of antibodies with high specificity and sensitivity, supporting research on poultry reproductive physiology and reproductive performance, and providing new ideas for breeding technology.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- YANGZHOU UNIV
- Filing Date
- 2025-07-25
- Publication Date
- 2026-07-17
AI Technical Summary
The lack of antibodies on the market for detecting chicken FSHR protein has severely hampered research on the function of chicken FSHR protein and affected the improvement of poultry production and breeding technologies.
Chicken FSHR polyclonal antibodies were prepared by expressing the target sequence (SEQ ID NO: 1) in prokaryotes, amplifying the chicken FSHR gene using PCR, constructing a prokaryotic recombinant expression plasmid, inducing expression and purification, separating and purifying serum after animal immunization, and preparing specific polyclonal antibodies.
This improves the sensitivity and specificity of the antibody, enabling it to effectively recognize endogenous chicken FSHR antigens in cells. This provides a theoretical basis for studying the structure and function of chicken FSHR proteins, and supports research on poultry reproductive physiology and reproductive performance, as well as breeding improvements.
Smart Images
Figure CN120829512B_ABST