A stem cell culture vessel with an interchangeable cylinder

Abstract

This invention discloses a stem cell culture vessel with an adjustable cylinder, specifically relating to the field of stem cell culture vessel technology. It includes a culture chamber, a controller, a micro-electric cylinder, and a distribution and adjustment assembly. The distribution and adjustment assembly comprises multiple collars, multiple connecting ropes, a linkage column, and a tilting counterweight plate. Through this distribution and adjustment assembly, the tops of multiple connecting ropes can move down synchronously, allowing multiple culture vessels to quickly and synchronously tilt and move down to the outside of the culture chamber. This significantly saves operation time and effort, enabling the removal of multiple culture vessels from the culture chamber at once. This facilitates centralized adjustment by operators, effectively improving work efficiency and meeting the needs of replacing multiple culture vessels on a large scale. It achieves the advantage of high-efficiency replacement, thus solving the problems of difficulty in simultaneously removing and adjusting a large number of internal stem cell culture vessels for replacement, and the low efficiency of synchronous replacement.

Description

Technical Field

[0001] This invention relates to the field of stem cell culture vessel technology, and more specifically, to a stem cell culture vessel with an interchangeable cylinder. Background Technology

[0002] Cell research projects vary greatly in scale, ranging from basic research with small numbers of cells to large-scale cell production for clinical treatment, requiring different culture volumes. The interchangeable tank design allows the culture vessel to easily adapt to different volumes. Whether it's small-scale laboratory research requiring only a small number of cells for mechanism exploration or drug screening, or large-scale clinical-grade cell production needing a large number of cells to meet patient treatment needs, the appropriate tank can be used instead of replacing the entire culture vessel, reducing equipment purchase costs.

[0003] Among existing published documents, patent publication number CN219079552U discloses a combined stem cell culture vessel. This technology includes a limiting component inside the outer vessel for maintaining a stable connection between a first connecting member and a second connecting member, as well as a driving component for rotating the first connecting member. This application has the effect of stirring the culture vessel and improving its adaptability. However, this technology still has the following problems: when replacing stem cell culture vessels, due to the large number of culture vessels, it is difficult to simultaneously remove and adjust a large number of internal stem cell culture vessels for replacement. Therefore, the efficiency of simultaneous replacement of stem cell culture vessels is low, making it difficult to achieve efficient replacement. Summary of the Invention

[0004] To overcome the aforementioned deficiencies of the prior art, the present invention provides the following technical solution: a stem cell culture tank with an adjustable cylinder, comprising a culture chamber and a controller. A miniature electric cylinder is fixedly connected to the upper surface of the culture chamber, and a distribution and adjustment assembly is installed inside the culture chamber. The distribution and adjustment assembly includes multiple collars installed inside the culture chamber. Multiple connecting ropes are fixedly connected to the lower surface of each collar. A linkage column is provided on one side of one of the connecting ropes. Two collars are fixedly connected to the linkage column. An inclined counterweight plate is fixedly connected to the bottom end of each connecting rope. Multiple support blocks are fixedly connected to one inclined surface of the inclined counterweight plate. A culture tank body is slidably connected to the inner wall of each support block. A stopper block is fixedly installed at the bottom end of the inclined counterweight plate, and the stopper block is slidably connected to the culture chamber.

[0005] Preferably, the plurality of collars are arranged sequentially from top to bottom, with a gap between two collars. The bottom surface of the inclined counterweight plate is lower than the top surface of the inclined counterweight plate, the outer wall of the plug is a smooth surface, and the plurality of inclined counterweight plates are arranged in a circular ring at equal intervals.

[0006] The controller is electrically connected to the miniature electric cylinder. A guide post is slidably connected to the inner wall of the collar, and a fixing post is fixedly connected to the top of the guide post; a protective inner plate is fixedly connected to the bottom of the inner wall of the incubator, and a connecting support block is fixedly installed at the top of the miniature electric cylinder.

[0007] The bottom of the incubator is fixedly connected to a base plate, which is used to support the incubator. The bottom of the connecting strip is fixedly connected to a base block.

[0008] When this technology is used, the controller starts the micro electric cylinder. The output end of the micro electric cylinder drives the top collar to move down, the linkage column drives the other collars to move down synchronously, the bottom end of the connecting rope drives the tilting counterweight plate to tilt down, the support block drives the culture tank to tilt down, and the tilting counterweight plate drives the plug block to tilt down. Multiple culture tanks can be quickly moved to the outside of the incubator, and the operator can swap multiple culture tanks.

[0009] Preferably, the outer wall of the incubator is slidably connected to two retaining rings; the outer wall of each retaining ring is provided with a connecting strip, and both retaining rings are fixedly connected to the connecting strip. A sleeve block is slidably connected to the upper surface of one of the retaining rings, the sleeve block is fixedly connected to the incubator, and the sleeve block is slidably connected to the connecting strip. A positioning post is fixedly installed at the top of the connecting strip, and a sleeve strip is rotatably connected to the outer wall of the positioning post. A limiting ring is rotatably connected to the upper surface of the sleeve strip, and the limiting ring is fixedly connected to the positioning post. A gripping post is provided on one side of the limiting ring, and the gripping post is fixedly connected to the sleeve strip. A limiting block with a polygonal cross-sectional shape is fixedly installed at the top of the gripping post. The sleeve strip is rotatably connected to the incubator, and the cross-sectional area of ​​the limiting block is larger than the cross-sectional area of ​​the gripping post.

[0010] When using this technology, the user holds the device on the outer wall of the holding column, and the limiting block limits the upper surface of the hand. The connecting strip rotates along the upper surface of the incubator. The connecting strip rotates on the outer wall of the positioning column. When the lower surface of the connecting strip no longer contacts the upper surface of the incubator, the connecting strip slides down along the inner wall of the sleeve block under the action of gravity. The connecting strip drives the bottom block to move down, and the retaining ring no longer blocks the plug.

[0011] Preferably, an inclined column is provided below the inclined counterweight plate; the inclined column is fixedly connected to the incubator, and an inclined slider is slidably connected to the outer wall of the inclined column, and the inclined slider is fixedly connected to the inclined counterweight plate. A connecting block is fixedly installed on the lower surface of the inclined slider, and a connecting column is fixedly connected to the bottom end of the connecting block. A counterweight block is welded to the bottom end of the connecting column. A gap is provided between the inclined column and the inclined counterweight plate, and the outer wall of the inclined column and the inner wall of the inclined slider are both smooth surfaces; the vertical cross-sectional shape of the inclined slider is circular. The counterweight block is used to counterweight the connecting column, and the cross-sectional area of ​​the counterweight block is larger than the cross-sectional area of ​​the connecting column.

[0012] When this technology is used, as the tilting counterweight plate tilts and moves downward, the tilting slider moves downward along the outer wall of the tilting column, and the connecting column drives the counterweight block to tilt and move downward, so that the top position of the tilting counterweight plate achieves strong gravity tilting counterweight, stably realizing the tilting and downward movement of the tilting counterweight plate. Multiple culture tanks can be stably tilted and moved downward to the outside of the incubator, and multiple culture tanks can be swapped.

[0013] The technical effects and advantages of this invention are as follows:

[0014] 1. This invention utilizes a distributed replacement component. A controller activates a micro electric cylinder, and through the linkage of a collar and a linkage column, the tops of multiple connecting ropes move down synchronously, thereby driving multiple culture tanks to quickly and synchronously tilt and move down to the outside of the incubator. This greatly saves operation time and effort. Secondly, it facilitates batch replacement, allowing multiple culture tanks to be moved out of the incubator at once, making it convenient for operators to perform centralized replacement, effectively improving work efficiency, meeting the needs of replacing multiple culture tanks on a large scale, and achieving efficient replacement.

[0015] 2. This invention uses a hand-held gripping column to drive the connecting strip to rotate in multiple directions, easily separating the connecting strip from the upper surface of the incubator. Secondly, the incubator support block provides a stable foundation for the connecting strip to slide down, ensuring that the connecting strip can stably drive the bottom block and the retaining ring to move down. The function is precise. After the retaining ring moves down, it no longer obstructs the plug, accurately achieving the unobstructed operation. The entire peripheral separation process is coherent and efficient, with all components working together to separate.

[0016] 3. The present invention uses an inclined counterweight plate to drive an inclined slider to move downwards at an angle. The inclined slider moves downwards at an angle along the outer wall of the inclined column. The connecting block drives the connecting column to move downwards at an angle. The counterweight block and the connecting column can achieve counterweight processing and stably achieve the downward movement of the inclined counterweight plate. Multiple culture tanks can be stably moved downwards at an angle to the outside of the incubator, thereby realizing the replacement of multiple culture tanks. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure of the stem cell culture tank with an adjustable cylinder according to the present invention.

[0018] Figure 2 This is a schematic diagram of the vertical cross-sectional structure of the stem cell culture tank with an adjustable cylinder according to the present invention.

[0019] Figure 3 This is a partial structural diagram of the connection between the fixed column and the guide column of the present invention.

[0020] Figure 4 This is a partial structural diagram of the vertical cross-section at the connection point between the connecting rope and the inclined counterweight plate of the present invention.

[0021] Figure 5 This is a schematic diagram of a partial section of the structure at the connection between the retaining ring and the connecting strip of the present invention.

[0022] Figure 6 This is a bottom view of the stem cell culture tank with an adjustable cylinder according to the present invention.

[0023] Figure 7 For the present invention Figure 5 Enlarged structural diagram at point A in the middle.

[0024] Figure 8 For the present invention Figure 4 Enlarged structural diagram at point B.

[0025] The attached diagram is labeled as follows: 1. Incubator; 2. Miniature electric cylinder; 3. Collar; 4. Connecting rope; 5. Linkage column; 6. Inclined counterweight plate; 7. Support block; 8. Culture tank body; 9. Guide column; 10. Controller; 11. Retaining ring; 12. Plug block; 13. Sleeve block; 14. Connecting strip; 15. Positioning column; 16. Sleeve strip; 17. Limiting ring; 18. Holding column; 19. Limiting block; 20. Inclined column; 21. Inclined slider; 22. Connecting block; 23. Connecting column; 24. Counterweight block; 25. Fixing column; 26. Base plate; 27. Base block; 28. Inner protective plate; 29. ​​Connecting support block. Detailed Implementation

[0026] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0027] As attached Figure 1 - Appendix Figure 8The diagram shows a stem cell culture tank with an adjustable cylinder. The adjustable stem cell culture tank is equipped with a distribution and replacement component. The distribution and replacement component can remove multiple culture tanks 8 from the culture chamber 1 at one time, which is convenient for operators to replace them in a centralized manner, effectively improving work efficiency and meeting the replacement needs of multiple culture tanks 8 on a large scale, thus achieving efficient replacement. The specific structural configuration of the distribution and replacement component is as follows.

[0028] In this embodiment, as shown in the appendix Figure 1 - Appendix Figure 4 As shown, a miniature electric cylinder 2 is fixedly connected to the upper surface of the incubator 1, and a distribution and switching assembly is installed inside the incubator 1. The distribution and switching assembly includes multiple collars 3 installed inside the incubator 1. Multiple connecting ropes 4 are fixedly connected to the lower surface of each collar 3. A linkage column 5 is provided on one side of one of the connecting ropes 4. Both collars 3 are fixedly connected to the linkage column 5. An inclined counterweight plate 6 is fixedly connected to the bottom end of each connecting rope 4. Multiple support blocks 7 are fixedly connected to one inclined surface of the inclined counterweight plate 6. A culture tank body 8 is slidably connected to the inner wall of each support block 7. A stopper block 12 is fixedly installed at the bottom end of the inclined counterweight plate 6, and the stopper block 12 is slidably connected to the incubator 1. The multiple collars 3 are arranged sequentially from top to bottom, with a gap between two collars 3. The bottom surface of the inclined counterweight plate 6 is lower than the top surface of the inclined counterweight plate 6. The outer wall of the stopper block 12 is a smooth surface. The multiple inclined counterweight plates 6 are arranged in a circular ring with equal spacing. The controller 10 is electrically connected to the miniature electric cylinder 2.

[0029] In this embodiment, as shown in the appendix Figure 1 - Appendix Figure 6 As shown, a guide post 9 is slidably connected to the inner wall of the collar 3, and a fixing post 25 is fixedly connected to the top of the guide post 9 so that the fixing post 25 can be supported on the top of the guide post 9; a protective inner plate 28 is fixedly connected to the bottom of the inner wall of the incubator 1 so that the protective inner plate 28 can provide reinforcement to the bottom of the inner wall of the incubator 1. A connecting support block 29 is fixedly installed on the top of the micro electric cylinder 2 so that the connecting support block 29 can support the top of the micro electric cylinder 2. A base plate 26 is fixedly connected to the bottom of the incubator 1, and the base plate 26 is used to support the incubator 1. A base block 27 is fixedly connected to the bottom of the connecting strip 14 so that the base plate 26 can provide reinforcement support to the bottom of the incubator 1, increasing the stability of the incubator 1 during use, while the base block 27 provides reinforcement to the bottom of the connecting strip 14.

[0030] In this embodiment, as shown in the appendix Figure 4 - Appendix Figure 7As shown, the outer wall of the incubator 1 is slidably connected with two retaining rings 11; the outer wall of the retaining ring 11 is provided with a connecting strip 14, and both retaining rings 11 are fixedly connected to the connecting strip 14. A sleeve block 13 is slidably connected to the upper surface of one of the retaining rings 11. The sleeve block 13 is fixedly connected to the incubator 1 and slidably connected to the connecting strip 14. A positioning post 15 is fixedly installed at the top of the connecting strip 14, and a sleeve strip 16 is rotatably connected to the outer wall of the positioning post 15.

[0031] A limiting ring 17 is rotatably connected to the upper surface of the socket strip 16, and the limiting ring 17 is fixedly connected to the positioning post 15. A holding post 18 is provided on one side of the limiting ring 17, and the holding post 18 is fixedly connected to the socket strip 16. A limiting block 19 with a polygonal cross-sectional shape is fixedly installed at the top of the holding post 18. The socket strip 16 is rotatably connected to the incubator 1, and the cross-sectional area of ​​the limiting block 19 is larger than the cross-sectional area of ​​the holding post 18.

[0032] In this embodiment, as shown in the appendix Figure 8 As shown, an inclined column 20 is provided below the inclined counterweight plate 6; the inclined column 20 is fixedly connected to the incubator 1, and an inclined slider 21 is slidably connected to the outer wall of the inclined column 20, and the inclined slider 21 is fixedly connected to the inclined counterweight plate 6. A connecting block 22 is fixedly installed on the lower surface of the inclined slider 21, and a connecting column 23 is fixedly connected to the bottom end of the connecting block 22. A counterweight block 24 is welded to the bottom end of the connecting column 23. There is a gap between the inclined column 20 and the inclined counterweight plate 6, and the outer wall of the inclined column 20 and the inner wall of the inclined slider 21 are both smooth surfaces; the vertical cross-section of the inclined slider 21 is circular, and the counterweight block 24 is used to counterweight the connecting column 23. The cross-sectional area of ​​the counterweight block 24 is larger than the cross-sectional area of ​​the connecting column 23.

[0033] The working principle of the stem cell culture tank with an adjustable cylinder of the present invention is as follows:

[0034] Step 1: When using the culture, in a pollution-free laboratory environment, operators must wear sterile clothing and protective clothing. All equipment must be thoroughly disinfected before entering the laboratory. Place the stem cell culture dishes inside multiple culture tanks 8, which are located inside the incubator 1, so that multiple stem cell culture dishes can be placed and cultured inside the incubator 1.

[0035] Step 2: During peripheral separation, the hand is gripped on the outer wall of the gripping column 18, with the limiting block 19 limiting the upper surface of the hand. Then, the gripping column 18 is rotated, causing the connecting strip 16 to rotate. The connecting strip 16 rotates along the upper surface of the incubator 1, and simultaneously rotates along the lower surface of the limiting ring 17. The connecting strip 16 also rotates on the outer wall of the positioning column 15, and at the same time, it rotates at the top of the connecting strip 14. When the connecting strip 16... When the lower surface of the incubator 1 no longer contacts the upper surface of the incubator 1, the connecting strip 14 slides down along the inner wall of the sleeve block 13 under the action of gravity. At the same time, the incubator 1 supports the sleeve block 13, increasing the stability of the sleeve block 13. The connecting strip 14 also drives the bottom block 27 to move down, and the connecting strip 14 also drives the two retaining rings 11 to move down. The retaining rings 11 slide between the retaining rings 11 and the plug block 12. The retaining rings 11 no longer block the plug block 12, making it convenient for the plug block 12 to discharge from the area blocked by the retaining rings 11.

[0036] Step 3: During the distribution and replacement, the micro electric cylinder 2 is activated by the controller 10. The bottom block 27 is fixed to the bottom of the micro electric cylinder 2. The output end of the micro electric cylinder 2 drives the topmost collar 3 to move down. The collar 3 drives the linkage column 5 to move down. The linkage column 5 drives the other collars 3 to move down synchronously. In this way, the collars 3 drive the top ends of multiple connecting ropes 4 to move down synchronously. The bottom ends of the connecting ropes 4 drive the tilting counterweight plate 6 to tilt down. The tilting counterweight plate 6 drives multiple support blocks 7 to tilt down. At the same time, the support blocks 7 drive the culture tank 8 to tilt down. In this way, the tilting counterweight plate 6 drives the plug block 12 to tilt down. The plug block 12 presses against the inner wall of the incubator 1 and tilts down. In this way, multiple culture tanks 8 can be quickly moved to the outside of the incubator 1. The operator can then replace multiple culture tanks 8. This allows multiple culture tanks 8 inside the incubator 1 to be quickly moved to the outside of the incubator 1, thus realizing the replacement operation of culture tanks 8 outside the incubator 1.

[0037] Step 4: During the tilting guide counterweight operation, when the tilting counterweight plate 6 tilts downward, it drives the tilting slider 21 to tilt downward. The tilting slider 21 tilts downward along the outer wall of the tilting column 20, which in turn drives the connecting block 22 to tilt downward. The connecting block 22 then drives the connecting column 23 to tilt downward, which in turn drives the counterweight block 24 to tilt downward. The counterweight block 24 and the connecting column 23 achieve counterweight processing, thereby enabling a strong gravity tilting counterweight at the top of the tilting counterweight plate 6, stably achieving the tilting downward movement of the tilting counterweight plate 6. This allows multiple culture tanks 8 to stably tilt downward to the outside of the incubator 1, thus enabling the multiple culture tanks 8 to be stably tilted downward. The process is now reversed. A new culture tank 8 is placed inside the support block 7, and a new culture dish is placed inside the new culture tank 8. After use, the controller 10 starts the micro electric cylinder 2, which drives the collar 3 to move the linkage column 5 upward. The bottom end of the connecting rope 4 drives the tilting counterweight plate 6 to tilt upward. The tilting counterweight plate 6 drives multiple support blocks 7 to tilt upward. At the same time, the support blocks 7 drive the culture tank 8 to tilt upward. In this way, the tilting counterweight plate 6 drives the plug block 12 to tilt upward. After the hand pulls the holding column 18 upward, the holding column 18 is rotated. The holding column 18 drives the lower surface of the connecting strip 16 to contact the upper surface of the culture box 1 to complete the snap-fit ​​installation. This allows the retaining ring 11 to block and seal the plug block 12, and the culture process can then begin.

[0038] All contents not described in detail in the specification are existing technologies known to those skilled in the art, and the model parameters of each electrical appliance are not specifically limited; conventional equipment can be used. Electrical control components not mentioned in this technical solution are not shown in the figures because they are existing technologies, and will not be described here.

[0039] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A stem cell culture tank with an adjustable cylinder, comprising a culture chamber (1) and a controller (10), wherein a miniature electric cylinder (2) is fixedly connected to the upper surface of the culture chamber (1), characterized in that: The incubator (1) is equipped with a distribution exchange component; The distribution and switching assembly includes multiple collars (3) installed inside the incubator (1). Multiple connecting ropes (4) are fixedly connected to the lower surface of each collar (3). A linkage column (5) is provided on one side of one of the connecting ropes (4). Both collars (3) are fixedly connected to the linkage column (5). An inclined counterweight plate (6) is fixedly connected to the bottom end of each connecting rope (4). Multiple support blocks (7) are fixedly connected to one inclined surface of the inclined counterweight plate (6). A culture tank (8) is slidably connected to the inner wall of each support block (7). A stopper (12) is fixedly installed at the bottom end of the inclined counterweight plate (6), and the stopper (12) is slidably connected to the incubator (1); The bottom surface of the inclined counterweight plate (6) is lower than the top surface of the inclined counterweight plate (6), the outer wall of the plug (12) is a smooth surface, and the multiple inclined counterweight plates (6) are arranged in a circular ring at equal intervals. The controller (10) is electrically connected to the miniature electric cylinder (2); The inner wall of the collar (3) is slidably connected to a guide post (9), and the top end of the guide post (9) is fixedly connected to a fixing post (25). An inclined column (20) is provided below the inclined counterweight plate (6). The inclined column (20) is fixedly connected to the incubator (1). An inclined slider (21) is slidably connected to the outer wall of the inclined column (20). The inclined slider (21) is fixedly connected to the inclined counterweight plate (6). A connecting block (22) is fixedly installed on the lower surface of the inclined slider (21). A connecting column (23) is fixedly connected to the bottom end of the connecting block (22). A counterweight block (24) is welded to the bottom end of the connecting column (23).

2. The stem cell culture vessel with an interchangeable cylinder as described in claim 1, characterized in that: Multiple collars (3) are arranged sequentially from top to bottom, with a gap between two collars (3).

3. The stem cell culture vessel with an adjustable cylinder according to claim 1, characterized in that: The bottom of the inner wall of the incubator (1) is fixedly connected to a protective inner plate (28), and the top of the micro electric cylinder (2) is fixedly installed with a connecting support block (29).

4. The stem cell culture vessel with an adjustable cylinder according to claim 1, characterized in that: The outer wall of the incubator (1) is slidably connected to two retaining rings (11); The outer wall of the retaining ring (11) is provided with a connecting strip (14). Both retaining rings (11) are fixedly connected to the connecting strip (14). A sleeve block (13) is slidably connected to the upper surface of one of the retaining rings (11). The sleeve block (13) is fixedly connected to the incubator (1). The sleeve block (13) is slidably connected to the connecting strip (14). A positioning post (15) is fixedly installed at the top of the connecting strip (14). A sleeve strip (16) is rotatably connected to the outer wall of the positioning post (15). The upper surface of the socket strip (16) is rotatably connected to a limiting ring (17), and the limiting ring (17) is fixedly connected to a positioning post (15). A gripping post (18) is provided on one side of the limiting ring (17), and the gripping post (18) is fixedly connected to the socket strip (16). A limiting block (19) with a polygonal cross-sectional shape is fixedly installed at the top of the gripping post (18).

5. The stem cell culture vessel with an adjustable cylinder according to claim 4, characterized in that: The socket strip (16) is rotatably connected to the incubator (1), and the cross-sectional area of ​​the limiting block (19) is greater than the cross-sectional area of ​​the holding column (18).

6. The stem cell culture vessel with an interchangeable cylinder according to claim 1, characterized in that: There is a gap between the inclined column (20) and the inclined counterweight plate (6), and the outer wall of the inclined column (20) and the inner wall of the inclined slider (21) are both smooth surfaces; The vertical cross-section of the inclined slider (21) is circular.

7. The stem cell culture vessel with an interchangeable cylinder according to claim 1, characterized in that: The counterweight (24) is used to counterweight the connecting column (23), and the cross-sectional area of ​​the counterweight (24) is greater than the cross-sectional area of ​​the connecting column (23).

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