Method for cultivating cordyceps militaris by using turtles and soft-shelled turtles as parasitic bodies and wine product thereof

Abstract

The application discloses a method for cultivating cordyceps militaris by taking turtles and soft-shelled turtles as parasitic bodies and a wine product thereof. The method is characterized by taking turtles or soft-shelled turtles as carriers, taking food materials as auxiliary materials, and taking cordyceps militaris strains as a fungus source. Fresh and complete turtles or soft-shelled turtles are treated, and the internal organs and fat are removed. The internal organs are subjected to fat removal and odor removal treatment, combined with the auxiliary materials, and sterilized to obtain culture materials. The cordyceps militaris strains are combined with the culture materials to obtain culture medium. The culture medium is divided and packed into the shells of the turtles or soft-shelled turtles, and then placed in a culture device for cultivation. The cultivation is performed until the turtle or soft-shelled turtle shells are covered with cordyceps militaris fruiting bodies to obtain turtle or soft-shelled turtle cordyceps militaris complexes. The turtle or soft-shelled turtle cordyceps militaris complexes are soaked in baijiu to obtain turtle or soft-shelled turtle cordyceps militaris wine products. The method realizes cultivation of cordyceps militaris by taking turtles and soft-shelled turtles as parasitic bodies. The cultivated turtle or soft-shelled turtle cordyceps militaris complexes are novel and characteristic, and are also combined with various natural nutritional components such as turtles or soft-shelled turtles, cordyceps militaris and traditional Chinese medicinal materials.

Description

Technical Field

[0001] This invention relates to the technical field of Cordyceps militaris cultivation, specifically to a method for cultivating Cordyceps militaris using turtles and tortoises as parasites, and its alcoholic beverage products. Background Technology

[0002] Cordyceps militaris, also known as caterpillar fungus, is a complex of the stroma of the fungus Cordyceps sinensis (family Clavicipitaceae) parasitizing the larvae of moths (family Mothidae) and the larval corpse. It is a fungus with various active substances, including cordycepin, cordyceps polysaccharides, cordycepic acid, ergosterol, selenium, and nucleosides. It is a medicinal and edible fungus, considered a superior tonic. Currently, artificially cultivated Cordyceps militaris mainly falls into two categories: one is produced by artificially raising insect pupae using the fungus, and the second, the most common type on the market, is produced by artificially raising grains or wheat through fermentation. Cordyceps militaris is 5-8 cm long, golden or orange-yellow in color, and belongs to the same genus Cordyceps as Cordyceps sinensis (family Cordyceps genus) in the phylum of fungi, sharing similar medicinal and tonic effects.

[0003] Domestic and international research on species that have become induced cordyceps fruiting bodies suggests that *Cordyceps militaris* has comparable edible and medicinal value to traditional *Cordyceps sinensis*, with artificially cultivated *Cordyceps militaris* containing dozens of times more cordycepin than traditional *Cordyceps sinensis*. Modern medical research has proven that *Cordyceps militaris* contains 18 amino acids, 17 trace elements, and 12 vitamins (A, B1, B2, B6, B12, C, D, and E) in higher amounts than mushrooms, and possesses effects such as nourishing the lungs and kidneys, stopping bleeding and resolving phlegm, dilating the trachea, sedation, antibacterial activity, and lowering blood pressure. Research and testing worldwide have proven that *Cordyceps militaris* is the most ideal substitute for traditional *Cordyceps sinensis*.

[0004] Currently, most cultivation of Cordyceps militaris uses plants as carriers, utilizing the carbon and nitrogen sources found in plants to provide nutrients for its growth and development. By employing biotechnology, Cordyceps militaris and agricultural products can be organically integrated, leveraging the efficacy, value, and influence of Cordyceps militaris to significantly enhance the added value of agricultural products – a process of great significance. The primary animal carriers for Cordyceps militaris cultivation are silkworms, including tussah silkworms and mulberry silkworms. However, there are few reports on technologies using other animal bodies as carriers for Cordyceps militaris cultivation. Cultivating Cordyceps militaris using animal bodies is of great significance and application value for exploring valuable traditional Chinese medicine resources, developing new uses for animal resources, and increasing their added value. Therefore, research and development of technologies for cultivating Cordyceps militaris using other animal bodies as carriers is also of paramount importance.

[0005] Taking turtles and soft-shelled turtles as examples, turtles are amphibious animals. Due to their lifestyle and structural characteristics, turtle meat is rich in protein, fat, minerals, and other substances, making it highly nutritious and medicinal. The protein content of turtle muscle reaches 16.64%, with essential amino acids and umami amino acids accounting for 49.16% and 43.39% of the total amino acids, respectively. Glutamate (Glu) is the most abundant amino acid. The fat content of turtle muscle is 1.51%, with C18:1 as the main fatty acid composition, reaching 39.32%. This is followed by C16:0 and C22:6, with unsaturated fatty acids accounting for 76.83% of the total fatty acids. Turtle meat, especially the skirt-like part of the shell, is rich in collagen protein and has excellent yin-nourishing effects. Because turtle meat is high in protein and low in fat, it is very suitable for nourishing the elderly. The turtle shell is rich in various nutrients, which are the material basis for its efficacy. It contains collagen, an important component of human bones and connective tissue, which helps maintain bone toughness and elasticity. Meanwhile, tortoise shells also contain various amino acids, such as glycine, alanine, and proline. These amino acids are the basic raw materials for the human body to synthesize proteins and participate in the body's metabolism and physiological function regulation. In addition, tortoise shells also contain various minerals such as calcium and phosphorus. Calcium is a key element for maintaining bone health, while phosphorus plays an important role in energy metabolism and acid-base balance. Traditional Chinese medicine believes that tortoises and deer are spiritual and long-lived. Tortoises, born with the Qi of the North, are the most Yin of all creatures, circulating along the Ren meridian, connecting the heart Qi above and the kidney meridian below. Therefore, they can nourish Yin and invigorate blood, relax muscles and treat fatigue, moisten the lungs and stop coughing. They can treat contractures of the four limbs or long-term paralysis that cannot be cured, and are also used to treat gout. Tortoise meat is sweet, sour, and warm in nature, entering the liver, lung, and kidney meridians. It can be used for tuberculosis, cough, hemoptysis, hematemesis, dysentery, polyuria, enuresis, intestinal bleeding, muscle and bone pain, uterine prolapse, diabetes, and other conditions. Turtle shell, when boiled into turtle paste, has even greater effects in nourishing yin and suppressing yang, making it an important tonic and health supplement suitable for many symptoms such as yin deficiency anemia, uterine bleeding, neurasthenia, and general weakness. Turtle gallbladder is bitter and cold in nature, with effects of clearing heat and detoxifying, and promoting bile secretion and improving eyesight. Furthermore, turtle meat can treat headaches and dizziness caused by post-concussion syndrome; turtle blood can invigorate blood circulation and heal injuries, and is used to treat blood deficiency and weakness, chronic cough with hemoptysis, chronic diarrhea with bloody stools, feverish bone steaming, and lower back and leg pain. The turtle, commonly known as the soft-shelled turtle or terrapin, is an oviparous amphibian. In traditional Chinese medicine, turtle shell (the shell of the soft-shelled turtle) is considered to have multiple medicinal values ​​and can be used to treat various diseases, such as malaria, blood stasis and lower back pain, and infantile hypochondriac pain. Turtle shell is salty and neutral in nature, non-toxic, and is often used as a yin-tonifying and qi-tonifying herb. Soft-shelled turtles are rich in protein, with up to 16.5 grams of protein per 100 grams of meat. They also contain high levels of fat, vitamins, calcium, phosphorus, iron, and other trace elements, as well as nutrients such as animal collagen and keratin. They are considered to have the effects of nourishing yin and reducing internal heat, fighting cancer, purifying blood and lowering blood pressure, promoting blood circulation and removing blood stasis, clearing heat and detoxifying, softening and dispersing nodules, tonifying the kidneys and strengthening yang, nourishing blood and calming the mind, and beautifying the skin. Therefore, they are regarded as a highly nutritious tonic food.Ancient Chinese records state that turtles can "replenish injuries, strengthen yang energy, and greatly replenish yin deficiency," and they have long been regarded as a nourishing and health-promoting food. The nutritional value of turtles is universally recognized; they are a prized aquatic product, a delicacy at high-end banquets, and a popular and beloved food. Not only are they delicious, high in protein, and low in fat, but they are also a nourishing delicacy containing various vitamins and trace elements. They possess the flavor of five types of meat—chicken, venison, beef, pork, and fish—and are known as "five-flavored meats." Currently, there is a considerable scale and market for tonics and strengthening agents made primarily from turtles both domestically and internationally, including products such as Chinese Turtle Essence, Chinese Turtle Active Nutritional Dew, Chinese Turtle Tonic Pills, and Whole Turtle Powder. If Cordyceps militaris can be cultivated using turtles or tortoises as a medium, a novel and unique product with outstanding efficacy will be created. Turtles and tortoises themselves have certain medicinal value in traditional medicine, while Cordyceps militaris has the effects of tonifying the lungs and kidneys, stopping bleeding and resolving phlegm. The combined turtle and tortoise Cordyceps militaris product will have a wider range of effects in food, health care and medicine.

[0006] However, research has revealed significant differences between plant and animal-based materials in terms of morphology, tissue structure, nutritional value, and efficacy. Furthermore, the physiological characteristics of animals differ greatly from traditional culture media, resulting in complex internal environments. Accurately simulating suitable conditions for the growth of Cordyceps militaris fungus presents a challenge. If turtles and tortoises are used as carriers to cultivate Cordyceps militaris in the same way as plant-based methods, it is difficult to cultivate abundant Cordyceps militaris mycelia. Therefore, it is necessary to overcome the technical difficulties arising from using animal-based carriers to cultivate Cordyceps militaris. Summary of the Invention

[0007] The purpose of this invention is to address the problems existing in the prior art by providing a method for cultivating Cordyceps militaris using turtles and tortoises as parasites, and its wine products. This technology uses bio-fermentation to organically combine the components of turtles or tortoises, Cordyceps militaris, and various other ingredients. The resulting turtle or tortoise Cordyceps militaris complex is not only novel and unique, but also combines various natural nutrients from turtles or tortoises, Cordyceps militaris, and traditional Chinese medicine. The prepared turtle and tortoise Cordyceps militaris wine contains abundant cordycepin, cordyceps polysaccharides, proteins, amino acids, total flavonoids, and other effective components, resulting in significant health benefits.

[0008] This invention is achieved using the following technical solution: A method for cultivating Cordyceps militaris using turtles or tortoises as parasites involves using turtles or tortoises as carriers, food ingredients as supplementary materials, and Cordyceps militaris spores as the inoculum. First, fresh, intact turtle or tortoise bodies are processed to remove internal organs and fat. Then, the internal organs are degreased and deodorized before being combined with the supplementary materials and sterilized to obtain a culture medium. Next, the Cordyceps militaris spores and the culture medium are combined to form a culture substrate. The culture substrate is then dispensed into turtle or tortoise shells and placed in a cultivation device for cultivation until the turtle or tortoise shells are covered with Cordyceps militaris fruiting bodies, thus obtaining a turtle or tortoise Cordyceps militaris complex.

[0009] Further preferred: The ingredients include one or more of the following: grains, miscellaneous grains, fruits, vegetables, animal meat, and Chinese medicinal herbs.

[0010] A further preferred method for processing fresh, intact turtle or tortoise carcasses is as follows: (1) Select a fresh and complete turtle or tortoise body, then drill a few venting holes and air holes on the back, and drill a few air holes on the abdomen. Then remove the internal organs and fat from the venting holes.

[0011] (2) Place the turtle or tortoise shell with the internal organs and fat removed into a cooking container, add water to submerge the turtle or tortoise shell, then heat until the water boils and continue cooking for 5-15 minutes to remove the fat remaining in the turtle's body for later use.

[0012] (3) Place the water and star anise together in a cooking container at a ratio of 10 kg water and 0.5-1 kg star anise. Then heat the water in the container until it boils and continue heating for 5-10 minutes to obtain a star anise solution. Then soak the turtle or tortoise shell that has been degreased in the star anise solution for 10-30 minutes before use.

[0013] (4) Place the turtle or tortoise shell from the previous step into a sterilization device for sterilization.

[0014] A further preferred embodiment is that the turtle body is placed in a refrigeration device and refrigerated until it is frozen before the bacterial venting and ventilation vents are made. This makes the turtle body hard and allows the bacterial venting and ventilation vents to be made on the back and abdomen of the turtle body. This ensures that the venting is intact and does not damage the surrounding tissue, thus maintaining the firmness and overall appearance of the turtle body and not affecting the bacterial venting layout.

[0015] A further preferred embodiment: The preparation process of the culture medium is as follows: (1) Clean the internal organs of the turtle or tortoise to remove blood, and then remove the gallbladder and the fat adhering to the internal organs for later use.

[0016] (2) Place the offal in a cooking container, submerge it in water, heat it until the water boils, and continue cooking for 5-10 minutes to remove any remaining fat from the offal.

[0017] (3) Place the water and star anise together in a cooking container at a ratio of 10 kg water and 0.5-1 kg star anise. Then heat the water in the container until it boils and continue heating for 5-10 minutes to obtain a star anise solution. Then soak the degreased viscera in the star anise solution for 10-30 minutes before use.

[0018] (4) According to the weight ratio of viscera and water of 1:3-5, put the viscera and water together in a pulping machine to pulp the viscera into a pulp for later use.

[0019] (5) Combine the above materials according to the ratio of 400-600 parts water, 200-400 parts visceral slurry, 90-100 parts tomatoes, and 40-60 parts potatoes and grind them into a raw material sauce. Then add 6-10 parts brown sugar and 1-3 parts peptone to the raw material sauce and stir well to obtain a nutrient solution for later use.

[0020] (6) Combine the above materials into auxiliary materials according to the ratio of 80-90 parts of ingredients, 8-12 parts of star anise seed bran, and 3-6 parts of silkworm pupa powder. Then, place the auxiliary materials and nutrient solution in a container according to the ratio of 1:1.2-1.5. Then, sterilize the auxiliary materials and nutrient solution according to conventional methods to obtain culture medium.

[0021] (7) In a sterile environment, mix the Cordyceps militaris liquid culture into the culture medium at a ratio of (3-5):(100-120) parts, and then stir evenly to obtain the culture medium. The above ratio is a weight fraction ratio.

[0022] Further preferred method: The steps for dispensing the culture medium into the shell of a turtle or tortoise are as follows: (1) Under sterile conditions, spray the liquid Cordyceps militaris inoculum evenly over the entire shell of the turtle or tortoise. (2) Under sterile conditions, dispense the culture medium mixed with the liquid Cordyceps militaris inoculum into the shell of the turtle or tortoise through the inoculum outlets set on the shell of the turtle or tortoise.

[0023] A further preferred embodiment: The cultivation device comprises an incubator, a cultivation rack, a light strip, an ozone generator, an ultrasonic atomizing humidifier, an exhaust fan, a humidity control instrument, a time relay, a time control switch, and an air humidity regulating instrument; wherein, the cultivation rack is placed inside the incubator, the ultrasonic atomizing humidifier is placed at the bottom of the incubator, the light strip is placed on the inner walls of the front, back, left, and right sides of the incubator, the oxygen outlet pipe of the ozone generator is connected to the inside of the incubator, the exhaust fan is installed at the top of the incubator, the operation of the ultrasonic atomizing humidifier is controlled by the humidity control instrument, the opening and closing of the light strip is controlled by the time relay, the operation of the ozone generator is controlled by the time control switch, and the operation of the exhaust fan is controlled by the air humidity regulating instrument.

[0024] Further preferred method: The cultivation process of the turtle or tortoise Cordyceps militaris complex in the cultivation device is as follows: (1) Device disinfection: Start the time control switch to run the ozone generator and disinfect the inside of the cultivation box. (2) Dark light cultivation: Place the turtle or tortoise shell containing the culture medium on the cultivation rack, and then carry out dark light cultivation until the Cordyceps militaris mycelium fills the culture medium inside the turtle or tortoise shell and even the entire turtle or tortoise shell is covered with mycelium. (3) Light cultivation: Start the time relay and turn on the light strip to make the turtle or tortoise shell uniformly exposed to light until all the mycelium on the surface of the turtle or tortoise shell changes color. (4) Scratching: 2-3 days after the mycelium changes color, scratch the mycelium to induce the germination of fruiting bodies. (5) Device water injection and disinfection: Inject clean water into the bottom of the cultivation box so that the water level submerges the ultrasonic atomizing humidifier, and then start the time control switch to run the ozone generator so that the ozone is disinfected by the ozone ventilation pipe through the ozone ventilation pipe. (6) Fruiting body cultivation: When the culture medium on the turtle or tortoise shell begins to germinate fruiting bodies, set the opening and closing time control parameters of the time relay according to the requirements, so that the time relay can effectively control the light strip according to the set opening and closing time. Set the humidity control parameters of the humidity control instrument according to the requirements, so that the humidity control instrument can effectively control the ultrasonic atomizing humidifier according to the set humidity control parameters, so that the humidity environment in the incubator meets the requirements. Set the opening and closing time control parameters of the time control switch according to the requirements, so that the time control switch can effectively control the ozone generator according to the set parameters, so that the ozone disinfection can be used to keep the inside of the incubator clean to meet the requirements. Set the humidity control parameters of the air humidity regulating instrument according to the requirements, so that the air humidity regulating instrument can effectively control the exhaust fan according to the set humidity control parameters, so that the humidity environment in the incubator meets the requirements. Through intermittent ozone disinfection and reasonable control of light and humidity, the turtle or tortoise is covered with Cordyceps militaris fruiting bodies to obtain a turtle or tortoise Cordyceps militaris complex.

[0025] A further preferred method is to soak the turtle or tortoise pupa cordyceps complex in baijiu (Chinese liquor) to obtain a turtle or tortoise pupa cordyceps wine product. The soaking steps are as follows: remove the mycelium and mycelium from the head, feet, and tail of the turtle or tortoise pupa cordyceps complex and set aside; then place the processed turtle or tortoise pupa cordyceps complex in a liquor bottle, and then pour 30-60 degree baijiu into the bottle to soak the turtle or tortoise pupa cordyceps wine product for more than 30 days.

[0026] The aforementioned octagon ( Illicium verum Hook.f.Star anise, also known as anise, is a plant belonging to the genus *Illicium* in the family Illicaceae. It is a globally renowned spice, especially important in Southeast Asia. Star anise primarily contains volatile oil (star anise oil), including trans-anetinonal acid, anisaldehyde, and anethole. Its aroma mainly comes from volatile anisaldehyde, which has a rich fragrance. Adding star anise to stewed or braised meat not only removes the gamey smell but also makes the meat more tender. Furthermore, star anise is a spice used both as food and medicine. The fruit is used medicinally, generally harvested in autumn or the following spring, steamed, and then dried. It is believed to have warming, dispersing, and qi-regulating effects. The *Medical Essentials* records that star anise nourishes the kidneys, soothes the liver, relieves stagnation, relaxes muscles, and treats beriberi. In addition, star anise is rich in nutrients, containing protein, fat, dietary fiber, and dehydrated pentose. Meanwhile, star anise contains various components that give it the ability to inhibit bacteria. For example, fennel oil, one of the main components of star anise, has antibacterial properties, particularly its main component, anethole. Star anise extract, a natural aromatic agent from the star anise plant, inhibits various fungi, including Candida, Aspergillus, Fusarium, Candida albicans, filamentous fungi, and yeasts. Furthermore, the volatile oil in star anise inhibits Staphylococcus aureus, Escherichia coli, and Proteus, while its decoction inhibits Mycobacterium tuberculosis and Bacillus subtilis. In addition, star anise contains active ingredients such as anethole and anisaldehyde, all of which have antifungal activity. Therefore, both the components naturally present in star anise and its various extracts exhibit antiseptic and antibacterial functions. Star anise contains special aromatic substances, such as anethole and fatty oils, which can emit a rich aroma. Moreover, fennel contains aldehydes, ethers, ketones, alkenes, and other components, and its unique and rich aroma can, to some extent, mask the original odor, gamey smell, and other fishy smell of meat and other ingredients.

[0027] The aforementioned star anise seed bran is a residue left over from extracting star anise oil using star anise seeds as raw material. Star anise seeds, also known as fennel seeds, are a common spice and traditional Chinese medicine with a unique aroma and flavor. They are rich in nutrients such as protein, fat, carbohydrates, vitamins, and minerals. According to testing by a qualified third-party professional institution, star anise seed bran contains 21.6% protein and 5.2% fat. Furthermore, it is rich in star anise components, which also possess significant antibacterial and preservative properties.

[0028] The application principle and process of the aforementioned culture device are as follows: 1. Application of a combination of humidity control instrument and ultrasonic atomizing humidifier: The humidity control instrument consists of a humidity controller and a humidity probe. The humidity probe is placed inside the incubator, and the humidity parameters inside the incubator are fed back to the humidity controller by the humidity probe. When using the humidity control instrument to control the humidity inside the incubator, first, the required humidity parameters are set on the humidity control instrument according to the humidity requirements of the incubator. When the humidity in the incubator is lower than the set parameters, the humidity controller will automatically activate the ultrasonic atomizing humidifier. The ultrasonic atomizing humidifier will then spray a mist-like water vapor that spreads throughout the space inside the incubator, causing the humidity in the incubator to rise continuously. When the humidity rises to the set humidity parameters... When the humidity level drops below the set value, the humidity control instrument automatically stops the ultrasonic atomizing humidifier. When the humidity inside the incubator falls below the set value, the instrument automatically restarts the ultrasonic atomizing humidifier to increase the ambient humidity. In short, the humidity inside the incubator is automatically controlled within the set range. This achieves a high degree of automation in humidity management during incubator production, significantly improving work efficiency. The ultrasonic atomizing humidifier uses a high-frequency oscillation of 17,000 times per second to atomize water into 1-5 micron ultrafine particles and negative oxygen ions, which are then diffused into the air, humidifying the air and generating abundant negative oxygen ions, thus quickly achieving uniform humidification of the environment.

[0029] 2. Application of Time Relay and LED Strip Combination: Time parameters are set on the time relay to control the switching state of the circuit. Through the control of the electromagnetic relay and the delay effect of the timing element, the circuit is closed or opened within the set time. The time relay effectively controls the LED strip according to the set on and off durations, using the light from the LED strip to induce and promote the growth and development of Cordyceps militaris, achieving optimal cultivation results. Since excessive or insufficient light is detrimental to the healthy growth of fruiting bodies, the time relay is set to automatically control the opening and closing of the LED strip based on a normal daily light cycle of approximately 8-12 hours to meet the growth needs of Cordyceps militaris, thus achieving automated and precise control of the cultivation environment's lighting.

[0030] 3. Application of Time Switch and Ozone Generator Combination: Based on requirements such as disinfection duration, relevant parameters are set for the time switch. Utilizing the built-in microcomputer chip and internal circuitry of the time switch, connected to the power supply and controlled electrical equipment, the circuit executes the switching operation according to the preset time, achieving precise control of the on / off time. Based on this principle, the time switch automatically and effectively controls the operation of the ozone generator. The ozone generator disinfects the space environment inside the incubator and the clean water injected into the bottom of the incubator, ensuring the cleanliness of the incubator meets requirements. This ensures that the turtles or tortoises cultivating Cordyceps militaris complex placed in the incubator are not contaminated, and achieves automated and precise control.

[0031] 4. Application of the combination of air humidity control instrument and exhaust fan: The air humidity control instrument consists of a humidity controller and a humidity probe. The humidity probe is placed inside the incubator, and the humidity parameters inside the incubator are fed back to the air humidity control instrument by the humidity probe. The air humidity inside the incubator is obtained by controlling the operation of the ultrasonic atomizing humidifier under the control of the humidity controller. When the ultrasonic atomizing humidifier controlled by the humidity controller operates, it brings the air humidity in the incubator to the user-set value. Although the humidity controller has stopped the operation of the ultrasonic atomizing humidifier, because the incubator is closed and there is water at the bottom, the air humidity in the incubator often continues to rise. For example, the user-set air humidity is 85%, but the air humidity in the incubator will continue to rise to 90% or higher and will not drop for a long time. Once the ambient humidity in the incubator is too high... High humidity levels can negatively impact the normal growth of Cordyceps militaris, so it's crucial to restore the humidity in the incubator to the manually set value as quickly as possible. An air humidity controller is used to operate the exhaust fan, which removes excessive humidity from the incubator. When using an air humidity controller to regulate the humidity in the incubator, first set the required humidity parameters on the controller. When the humidity in the incubator exceeds the set parameter, the controller will automatically activate the exhaust fan to remove excess humidity. When the humidity drops to the set parameter, the controller will immediately and automatically stop the exhaust fan. This achieves the ideal humidity level inside the incubator, which is more conducive to the growth of Cordyceps militaris and enables automated and precise control.

[0032] The beneficial effects of this invention are as follows: 1. The method for cultivating Cordyceps militaris fruiting bodies using whole turtles or tortoises as parasites proposed in this invention is novel in conception, scientifically and rationally laid out in terms of technology and process, and easy to implement.

[0033] 2. By implementing this invention, biotechnology is used to allow Cordyceps militaris to grow on the bodies of turtles or tortoises, enabling the organic optimization of various substances and resulting in a richer concentration of nutrients.

[0034] 3. By implementing this invention, several venting holes are drilled into the back of the turtle or tortoise. This not only facilitates the removal of the turtle's or tortoise's internal organs and fat through these venting holes, but also facilitates the dispensing of culture medium and the growth of Cordyceps militaris fruiting bodies. Furthermore, the rational distribution of these venting holes ensures that the Cordyceps militaris fruiting bodies grow evenly on the turtle or tortoise's body. Aerobic properties are a characteristic developed by Cordyceps militaris mycelium to adapt to its natural growth environment. During germination, the mycelium requires a small amount of air to meet its oxygen needs for respiration and other physiological activities. Drilling several ventilation holes into the back and abdomen of the turtle provides the necessary oxygen flow for mycelial growth, which is crucial for its healthy development. In addition, from a physiological and metabolic perspective, aerobic conditions are conducive to the aerobic respiration of Cordyceps militaris mycelium. Under aerobic conditions, the mycelium can more efficiently decompose and utilize nutrients in the culture medium, such as carbon sources (glucose, sucrose, etc.) and organic nitrogen (amino acids, peptone, etc.), converting them into energy and substances required for growth and development, thereby enabling the growth and development of the mycelium and ultimately producing healthy and robust Cordyceps militaris fruiting bodies.

[0035] 4. Unlike turtles, the back and belly of soft-shelled turtles are not as hard. Because soft-shelled turtles are relatively soft, directly drilling fungal and venting pores into their bodies can easily damage the surrounding tissue, affecting not only the fungal growth pattern but also the turtle's overall stability and appearance. By implementing this invention, the turtle that has been scalded to death is first placed in a refrigeration unit until it freezes and hardens. Only then are fungal and venting pores drilled into the back and belly. This ensures the pores are intact and avoids damaging the surrounding tissue, thus maintaining the turtle's stability and overall appearance without affecting the fungal growth pattern.

[0036] 5. By implementing this invention, the turtle or tortoise carcass with its internal organs removed is placed in a cooking container, then heated to boiling and continuously boiled for 5-15 minutes. The boiling water dissolves and removes any remaining fat in the turtle or tortoise carcass. Excessive fat content in turtles or tortoises not only leads to nutritional imbalance in the culture environment, affecting the absorption and utilization of other nutrients by the mycelium and thus inhibiting mycelial growth, but also increases the viscosity of the culture medium, alters the physical properties of the culture environment, and affects the diffusion of oxygen and other nutrients in the culture medium, resulting in localized hypoxia or insufficient nutrient supply to the mycelium, adversely affecting mycelial growth. Furthermore, fat affects the metabolic pathways of Cordyceps militaris mycelium because fatty acids act as signaling molecules, regulating the expression of specific genes within the mycelium, thereby affecting the synthesis and accumulation of active ingredients in the mycelium, such as promoting or inhibiting the synthesis of cordycepin and cordyceps polysaccharides. Furthermore, animal fat attracts the growth of some microorganisms. These microorganisms compete with Cordyceps militaris mycelium for nutrients and living space, which has a negative impact on the growth of Cordyceps militaris mycelium. These microorganisms also produce substances that are harmful to Cordyceps militaris mycelium and inhibit its growth.

[0037] 6. Both turtle and soft-shelled turtle meat have a very strong fishy odor, which significantly inhibits the growth of Cordyceps militaris mycelium. When the mycelium cannot grow normally, other harmful microorganisms will proliferate. These harmful microorganisms compete with the Cordyceps militaris mycelium for nutrients and living space, and the toxins they produce can affect the normal physiological functions of the mycelium and erode already grown mycelium, hindering its growth and development. Furthermore, the fishy odor can alter the pH of the culture environment, and Cordyceps militaris mycelium requires a suitable pH range for growth; exceeding this range can affect mycelial growth and metabolism. In addition, this distinctive fishy odor attracts pests or insects, which may also feed on the Cordyceps militaris mycelium or carry other pathogens, damaging the mycelium and further affecting its growth and development. By implementing this invention, an octagonal solution is prepared using water and octagonal powder. Then, the turtle or tortoise carcass that has undergone fat reduction is placed in the octagonal solution and soaked for 10-30 minutes. The aroma of octagonal powder is very strong and long-lasting, and does not easily dissipate. Soaking the turtle or tortoise carcass in the octagonal solution effectively intervenes in the fishy smell of the turtle or tortoise by utilizing the aroma of octagonal powder, allowing the Cordyceps militaris mycelium to have an affinity with the turtle or tortoise, which is conducive to the Cordyceps militaris mycelium feeding well, germinating and growing on the turtle or tortoise meat.

[0038] 7. By implementing this invention, the internal organs of turtles or tortoises are placed in a cooking container, then heated to boiling and continuously boiled for 5-10 minutes. The heat of the boiling water dissolves and removes the residual fat in the internal organs. Excessive fat content in the internal organs of turtles or tortoises can lead to nutritional imbalance in the culture environment, affecting the absorption and utilization of other nutrients by the mycelium, thus inhibiting mycelial growth. It also increases the viscosity of the culture medium, alters the physical properties of the culture environment, and affects the diffusion of oxygen and other nutrients in the culture medium, leading to localized hypoxia or insufficient nutrient supply to the mycelium, adversely affecting mycelial growth. Furthermore, fat affects the metabolic pathways of Cordyceps militaris mycelium because fatty acids act as signaling molecules, regulating the expression of specific genes within the mycelium, thereby affecting the synthesis and accumulation of active ingredients in the mycelium, such as promoting or inhibiting the synthesis of cordycepin and cordyceps polysaccharides. Furthermore, animal fat attracts the growth of some microorganisms. These microorganisms compete with Cordyceps militaris mycelium for nutrients and living space, which has a negative impact on the growth of Cordyceps militaris mycelium. These microorganisms also produce substances that are harmful to Cordyceps militaris mycelium and inhibit its growth.

[0039] 9. By implementing this invention, an anise solution is prepared using water and anise. Then, the degreased turtle or tortoise viscera are soaked in the anise solution for 10-30 minutes. The aroma of anise effectively intervenes in the fishy smell of the turtle or tortoise viscera, allowing the Cordyceps militaris mycelium to have affinity with the turtle or tortoise viscera. The Cordyceps militaris mycelium can then feed, germinate, and grow well in the culture medium containing the turtle or tortoise viscera.

[0040] 10. The complex body structure of turtles or tortoises, and the varying sizes of each individual, make it difficult to use their bodies as cultivation containers and precisely measure the amount of culture medium and nutrient solution inside. If too little culture medium and nutrient solution are added, the culture medium will not reach the pores when distributed within the turtle or tortoise, and the fruiting bodies will grow inside the turtle or tortoise. If too much culture medium and nutrient solution are added, the culture medium will overflow from the pores when distributed within the turtle or tortoise, and the culture medium distributed elsewhere in the turtle or tortoise will be compressed, which is not conducive to mycelial germination and growth. The mycelial growth cycle will be long, and the mycelium will become old, affecting the bioconversion rate. By implementing this invention, the turtle or tortoise body and the culture medium are sterilized separately. Then, in a sterile environment, the liquid Cordyceps militaris culture medium and the culture medium are mixed in proportion and stirred evenly to obtain a culture medium. Then, in a sterile environment, the culture medium containing the liquid Cordyceps militaris culture medium is dispensed into the turtle or tortoise body through the mycelial pores on its back. This allows for precise and appropriate placement of the culture medium into the turtle or tortoise body. During dispensing, the culture medium remains neither too tight nor too loose within the turtle or tortoise body, ensuring a reasonable distribution of the culture medium. This promotes rapid mycelial germination and growth, optimized fruiting body transformation, and... The invention ensures the integrity of the complex; simultaneously, it involves mixing the liquid Cordyceps militaris spawn into the culture medium for uniform mixing, ensuring complete contact between the spawn and the culture medium. Once the spawn germinates, the mycelium quickly fills the culture medium. Cordyceps mycelium is aerobic, and its distribution within the turtle or tortoise body results in a loose and non-compact culture medium. Furthermore, the vents on the turtle or tortoise body allow for good air circulation. The Cordyceps militaris mycelium distributed within the turtle or tortoise body can have excellent contact with the air, resulting in rapid mycelial growth, a short mycelial age, and thick, dense mycelium. This lays a solid foundation for cultivating high-quality Cordyceps militaris fruiting bodies with a high bioconversion rate.

[0041] 11. By implementing this invention, turtles or tortoises containing culture medium are placed in an incubator for cultivation. This releases Cordyceps militaris from its usual narrow cultivation containers such as bottles and boxes. In the spacious incubator, the mycelium and fruiting bodies of Cordyceps militaris have sufficient oxygen supply and growth space, effectively avoiding the defects of poor ventilation and excessive carbon dioxide accumulation during the cultivation and growth of Cordyceps militaris, which can lead to the inability of the stroma to differentiate normally and affect its growth and development. The incubator is equipped with LED light strips, an ozone generator, an ultrasonic humidifier, an exhaust fan, and an air filter. The LED light strips are precisely controlled by a time relay to induce and promote the growth and development of Cordyceps militaris, achieving optimal cultivation results. Since excessive or insufficient light is detrimental to the healthy growth of fruiting bodies, the time relay intermittently controls the LED light strips, providing approximately 8-12 hours of light per day to meet the growth requirements of Cordyceps militaris, achieving automated and precise control. A humidity control instrument controls the operation of the ultrasonic humidifier, ensuring it operates according to a set humidity value. When the humidity inside the incubator falls below the set value, the humidity control instrument... The ultrasonic atomizing humidifier automatically activates, generating a high-frequency oscillation of 17,000 times per second when powered on. This atomizes water into fine water particles of 1-5μm, humidifying the air and creating an optimal humidity environment for Cordyceps militaris growth within the incubator. This process is automated and precisely controlled. A timer switch controls the ozone generator, which disinfects the environment inside the incubator and the water injected at the bottom, ensuring the incubator remains clean and uncontaminated. This automated and precise control ensures that the turtles or tortoises in the incubator are not contaminated. Through intermittent ozone disinfection and precise control of light and humidity, the turtles or tortoises in the incubator safely and efficiently develop Cordyceps militaris fruiting bodies.

[0042] 12. The shells of turtles and soft-shelled turtles are hard, making them difficult for humans to eat directly. Even after being crushed, they are difficult for the body to absorb. This invention utilizes bio-fermentation technology to allow Cordyceps militaris mycelium to germinate and grow on turtle or soft-shelled turtle shells. During its growth, the mycelium penetrates the shell, effectively breaking down the internal components and converting large molecules into smaller ones, making them easily absorbed by the body. The essential components of the turtle or soft-shelled turtle shell are fully utilized. Furthermore, soaking turtles or soft-shelled turtles fermented with Cordyceps militaris in high-proof alcohol can quickly extract most or all of the internal components from the shell. Cooking also easily releases most or all of the components from the fermented Cordyceps militaris, making them even easier for the body to absorb and utilize.

[0043] 13. According to the tests conducted by the National Institute for Drug Control of the Ministry of Health, fermented traditional Chinese medicine requires only one twenty-eighth of the amount to achieve the efficacy of one dose of ordinary water extract. By implementing this invention, and utilizing bio-fermentation technology, the medicinal efficacy of fermented turtles or tortoises is significantly improved compared to traditional turtles or tortoises.

[0044] 14. By implementing this invention, small turtles or tortoises with poor commercial performance can be used as parasitic raw materials, enabling turtles or tortoises to expand their value without limit. This solves the problem of the post-production outlet for turtles or tortoises with poor commercial performance, drives the development of related industries, forms new economic growth points, and has huge economic and social benefits.

[0045] 15. By implementing this invention, Cordyceps militaris grows on the body of a turtle or tortoise. The turtle or tortoise retains its original shape under the intertwining of mycelia, forming a novel, unique, and beautiful combination of turtle and tortoise with Cordyceps militaris. This combination is placed in a bottle and then soaked in high-proof liquor, resulting in a golden-yellow liquor with a novel and distinctive shape, rich in nutrients, and eye-catching appeal. Because the turtle or tortoise's body has been penetrated by Cordyceps militaris mycelia, although it appears intact from the outside, the mycelia have actually penetrated all the tissues of the turtle or tortoise, effectively decomposing the turtle or tortoise itself, transforming large molecules into smaller molecules. After soaking in high-proof liquor, not only is a bottle of liquor with excellent color, aroma, taste, nutrition, and aesthetic appeal formed, but the high-proof liquor also quickly extracts the effective components of the turtle or tortoise and dissolves them in the liquor. When people drink the liquor, it's equivalent to effectively absorbing the effective components of the turtle or tortoise and Cordyceps militaris, resulting in better health benefits.

[0046] 16. Turtles and soft-shelled turtles themselves possess certain medicinal value in traditional medicine. Turtles can nourish yin and subdue yang, benefit the kidneys and strengthen bones, while soft-shelled turtles can nourish yin and clear heat, subdue yang and calm wind. Cordyceps militaris has the effects of tonifying the lungs and kidneys, stopping bleeding and resolving phlegm. Furthermore, the optimized culture medium combines several traditional Chinese medicinal materials, resulting in turtle or soft-shelled turtle cordyceps militaris products with broader applications in food, health care, and medicine. By implementing this invention, multiple natural nutrients from turtle or soft-shelled turtles, cordyceps militaris, and traditional Chinese medicinal materials are combined. Long-term consumption of the turtle or soft-shelled turtle cordyceps militaris obtained by this invention can maintain youthful vitality and delay aging.

[0047] 17. The meat, internal organs, and shell of turtles or tortoises are rich in nutrients such as protein, fat, and minerals, which provide abundant nutrition for the Cordyceps militaris growing on the turtle or tortoise and ensure a high bioconversion rate and high content of core components such as cordycepin, adenosine, and cordyceps polysaccharides.

[0048] 18. By implementing this invention, the turtle or tortoise pupa and Cordyceps militaris complex is soaked in baijiu (Chinese liquor), perfectly blending the turtle or tortoise, Cordyceps militaris, and other Chinese medicinal herbs with the baijiu. The resulting Cordyceps militaris liquor is smooth on the palate and has a delicate Cordyceps aroma, representing a significant innovation in the health-preserving liquor industry. The method for cultivating Cordyceps militaris and its liquor products using turtles or tortoises as host organisms, provided by this invention, is simple and easy to implement. The turtle or tortoise pupa and Cordyceps militaris liquor prepared using this method is not only excellent in color, aroma, taste, and nutrition, but also has a good mouthfeel. Furthermore, it contains abundant cordycepin, adenosine, cordyceps polysaccharides, protein, amino acids, and total flavonoids. Specifically, the cordycepin content is 432 mg / kg, adenosine 24.5 mg / kg, cordyceps polysaccharide content is 0.14%, protein 2.16%, amino acids 432%, and total flavonoids 1.27%. These high levels of active ingredients result in significant health benefits. Attached Figure Description

[0049] Figure 1 It is a real-life image of the fungal pores and ventilation pores made on the back of the turtle's body; Figure 2 This is a real-life image showing the locations of ventilation holes made on the underside of a turtle's body. Figure 3 It is a real-life image of the fungal pores and air pores made on the back of the turtle's body; Figure 4 This is a picture of a turtle with ventilation holes made on its abdomen. Figure 5 It is a real picture of the fruiting body of Cordyceps militaris growing on the body of a turtle; Figure 6 It is a real picture of the fruiting body of Cordyceps militaris growing on the body of a turtle; Figure 7 It is a real picture of the fruiting body of Cordyceps militaris growing on the body of a turtle; Figure 8 It is a real picture of the fruiting body of Cordyceps militaris growing on the body of a turtle; Figure 9 This is a picture of an alcoholic beverage made from a complex of Cordyceps militaris and Cordyceps sinensis. Detailed Implementation

[0050] The technical solutions of the invention will be clearly and completely described below with reference to the embodiments. The described embodiments are only a part of the present invention, and not all of the embodiments.

[0051] Example 1

[0052] The following method can be used to cultivate Cordyceps militaris using turtles as a parasite. The turtle body is used as a carrier, food is used as an auxiliary material, and Cordyceps militaris inoculum is used as the inoculum source. First, the fresh and intact turtle body is processed to remove the internal organs and fat. Then, the internal organs are degreased and deodorized, and combined with the auxiliary materials and sterilized to obtain the culture medium. Then, the Cordyceps militaris inoculum and the culture medium are combined to form a culture substrate. The culture substrate is then divided into turtle shells and placed in a culture device for cultivation. The turtle or tortoise shell is cultivated until it is covered with Cordyceps militaris fruiting bodies to obtain a turtle or tortoise Cordyceps militaris complex.

[0053] The above steps for processing fresh and intact turtle bodies are as follows: (1) Select fresh and intact turtle bodies with an age of 30 years. Then, according to the size of the turtle body, make 5-10 venting holes with a diameter of 20-40 mm and ventilation holes with a diameter of 3-5 mm on the back of the turtle body. Make 5-10 ventilation holes with a diameter of 3-5 mm on the abdomen of the turtle body. Then, remove the turtle's internal organs and fat from the venting holes for later use. (2) Place the turtle shell with the internal organs and fat removed in a cooking container, add water to submerge the turtle shell, then heat until the water boils and continue to cook for 10 minutes to remove the fat remaining in the turtle body for later use. (3) According to the ratio of 10 kg of clean water and 1 kg of star anise, put the clean water and star anise together in a cooking container, then heat until the water in the container boils and continue to heat for 10 minutes to obtain a star anise solution. Then, soak the turtle shell that has been degreased in the star anise solution for 30 minutes for later use. (4) Place the turtle body from the previous step into a high-pressure sterilizer and sterilize it for 25 minutes at a temperature of 121°C and a pressure of 0.15 MPa.

[0054] The preparation process of the above culture medium is as follows: (1) Clean the internal organs of the turtle to remove blood, and then remove the gallbladder and the fat adhering to the internal organs for later use. (2) Place the internal organs in a cooking container, add water to submerge the internal organs, then heat until the water boils and continue to cook for 10 minutes to remove the fat remaining in the internal organs for later use. (3) According to the ratio of 10 kg of water to 1 kg of star anise, place the water and star anise together in a cooking container, then heat until the water in the container boils and continue to heat for 10 minutes to obtain a star anise solution, and then place the degreased internal organs in the star anise solution to soak for 20 minutes for later use. (4) According to the weight ratio of internal organs to water of 1:5, place the internal organs and water together in a pulping machine to pulp the internal organs for later use. (5) Combine the above materials in the ratio of 550 parts water, 300 parts visceral fluid, 90 parts tomatoes, and 50 parts potatoes and grind them into a raw material sauce. Then add 8 parts brown sugar and 2 parts peptone to the raw material sauce and stir well to obtain a nutrient solution for later use. (6) Combine the above materials in the ratio of 85 parts wheat, 10 parts star anise seed bran, and 5 parts silkworm pupa powder to form an auxiliary material. Then place the auxiliary material and nutrient solution in a container in a ratio of 1:1.3. Then sterilize the auxiliary material and nutrient solution at a temperature of 121℃ and a pressure of 0.15 MPa for 25 minutes to obtain a culture medium. (7) In a sterile environment, mix the Cordyceps militaris liquid inoculum into the culture medium in a ratio of 5 parts:100 parts and stir well to obtain a culture medium. The above ratios are weight fractions.

[0055] The steps for dispensing the culture medium into the turtle shell are as follows: (1) Under sterile conditions, spray the liquid Cordyceps militaris inoculum evenly over the entire turtle shell. (2) Under sterile conditions, dispense the culture medium mixed with the liquid Cordyceps militaris inoculum into the turtle shell through the inoculum outlet holes set in the turtle shell. The culture medium in the turtle shell is level with the inoculum outlet holes, and the culture medium in the turtle shell is compacted without excessive compression.

[0056] The aforementioned cultivation device comprises an incubator, a cultivation rack, LED strip lights, an ozone generator, an ultrasonic humidifier, an exhaust fan, a humidity control instrument, a time relay, a time control switch, and an air humidity regulating instrument. The cultivation rack is placed inside the incubator; the ultrasonic humidifier is placed at the bottom of the incubator; the LED strip lights are placed on the inner walls of the incubator on the front, back, left, and right sides; the oxygen outlet pipe of the ozone generator is connected to the inside of the incubator; and the exhaust fan is installed at the top of the incubator. The humidity control instrument controls the operation of the ultrasonic humidifier; the time relay controls the on / off state of the LED strip lights; the time control switch controls the operation of the ozone generator; and the air humidity regulating instrument controls the operation of the exhaust fan.

[0057] The cultivation process of the Cordyceps militaris complex in the above-mentioned cultivation device is as follows: (1) Device disinfection: Start the time control switch to run the ozone generator and disinfect the inside of the cultivation box for 20 minutes. (2) Dark light cultivation: Place the turtle shell containing the culture medium on the cultivation rack, and then carry out dark light cultivation until the Cordyceps militaris mycelium fills the culture medium inside the turtle shell and even the entire turtle shell is covered with mycelium. (3) Light cultivation: Intelligent start time relay, according to the requirement of turning on the light strip for 10 hours every 12 hours, set the corresponding parameters in the time relay, so that the time relay automatically closes or opens every 12 hours, so that the light strip turns on for 10 hours and then automatically turns off, so that the turtle is evenly and reasonably exposed to light until all the mycelium on the surface of the turtle changes color. (4) Scratching: 2-3 days after the mycelium changes color, scratch the mycelium to induce the germination of fruiting bodies. (5) Water filling and disinfection of the device: Fill the bottom of the incubator with clean water so that the water level submerges the ultrasonic atomizing humidifier. Then, start the time control switch intelligently. According to the requirement that the ozone generator is turned on for 2 minutes every 24 hours and then automatically turned off, set the corresponding parameters on the time control switch. The time control switch will automatically execute the ozone generator's on and off operation so that the ozone can scientifically and reasonably disinfect the clean water filled at the bottom of the incubator.(6) Fruiting body cultivation: When fruiting bodies begin to germinate on the culture medium on the turtle's body, according to the requirement of turning on the light strip every 12 hours, turning it on for 12 hours and off for 12 hours a day, set the corresponding parameters on the time relay so that the time relay can effectively control the light strip according to the set on and off times; set the humidity control parameter of the humidity control instrument to 80% so that the humidity control instrument can effectively control the ultrasonic atomizing humidifier according to the set humidity control parameter so that the humidity environment in the incubator meets the requirements; according to the requirement that the ozone generator is turned on for 2 minutes and turned off for 24 hours, set the on and off time parameters on the time control switch so that the time control switch can effectively and reasonably control the ozone generator according to the set parameters, and use ozone disinfection to keep the inside of the incubator clean to meet the requirements; set the humidity control parameter of the air humidity control instrument to 85% so that the air humidity control instrument can effectively control the exhaust fan according to the set humidity control parameter. The humidity inside the incubator is controlled by a humidity control instrument that operates an ultrasonic atomizing humidifier. When the ultrasonic atomizing humidifier reaches the set humidity level, even after the instrument has stopped operating, the humidity level often continues to rise due to the enclosed design and the presence of water at the bottom of the incubator. For example, a set humidity level of 80% might rise to 85% or higher and remain high for an extended period. Excessive humidity negatively impacts the growth of Cordyceps militaris, so it's crucial to restore the humidity to the set value as quickly as possible. Using a humidity control instrument to operate an exhaust fan removes excess humidity, achieving an ideal humidity level that promotes Cordyceps militaris growth and allows for automated and precise control. Through intermittent ozone disinfection and proper control of light and humidity, a Cordyceps militaris complex is created by allowing Cordyceps militaris fruiting bodies to grow on the turtle's body.

[0058] The turtle and tortoise pupa cordyceps complex is soaked in baijiu (Chinese liquor) to obtain turtle and tortoise pupa cordyceps wine products. The soaking steps are as follows: remove the mycelium and mycelium from the turtle head, feet, and tail of the turtle and tortoise pupa cordyceps complex and set aside; then place the processed turtle and tortoise pupa cordyceps complex in a liquor bottle, and then pour 53-degree baijiu into the bottle to soak the turtle and tortoise pupa cordyceps wine products for more than 30 days.

[0059] Example 2

[0060] The following method can be used to cultivate Cordyceps militaris using turtles as a parasite. The turtle body is used as a carrier, food is used as an auxiliary material, and Cordyceps militaris inoculum is used as the inoculum source. First, the fresh and intact turtle body is processed to remove the internal organs and fat. Then, the internal organs are degreased and deodorized, and combined with the auxiliary materials and sterilized to obtain the culture medium. Then, the Cordyceps militaris inoculum and the culture medium are combined to form a culture substrate. The culture substrate is then divided into turtle shells and placed in a culture device for cultivation. The turtle or tortoise shell is cultivated until it is covered with Cordyceps militaris fruiting bodies to obtain a turtle or tortoise Cordyceps militaris complex.

[0061] The above steps for processing fresh and intact turtle bodies are as follows: (1) Select fresh and intact turtle bodies with an age of 20 years. Then, according to the size of the turtle body, make 5-10 venting holes with a diameter of 20-40 mm and ventilation holes with a diameter of 3-5 mm on the back of the turtle body. Make 5-10 ventilation holes with a diameter of 3-5 mm on the abdomen of the turtle body. Then remove the turtle's internal organs and fat from the venting holes for later use. (2) Place the turtle shell with the internal organs and fat removed in a cooking container, fill it with water to submerge the turtle shell, heat it until the water boils, and continue to cook for 10 minutes to remove the fat remaining in the turtle body for later use. (3) According to the ratio of 10 kg of water and 1.5 kg of star anise, put the water and star anise together in a cooking container, then heat until the water in the container boils, and continue heating for 10 minutes to obtain star anise solution. Then soak the turtle body that has been degreased in the star anise solution for 30 minutes to eliminate the fishy smell of the turtle with the aroma of star anise. Finally, take it out and let it dry for later use. (4) Place the turtle body that has been treated to remove the fishy smell in a high pressure sterilizer and sterilize it for 25 minutes at a temperature of 121℃ and a pressure of 0.15 MPa.

[0062] The preparation process of the above culture medium is as follows: (1) Turtle viscera treatment: Wash the collected turtle viscera with clean water to remove blood, and then remove the gallbladder and the fat adhering to the viscera for later use. (2) Visceral fat removal treatment: Place the viscera in a cooking container, add water to submerge the viscera, then heat until the water boils and continue to cook for 10 minutes to remove the fat remaining in the viscera. (3) Visceral odor removal treatment: Place the water and star anise together in a cooking container according to the ratio of 10 kg water to 1 kg star anise, then heat until the water in the container boils and continue to heat for 10 minutes to obtain a star anise solution. Then place the viscera that has been degreased into the star anise solution and soak for 20 minutes to remove the fishy smell of the viscera with the aroma of star anise. (4) Visceral pulping: Place the viscera and water together in a pulping machine according to the ratio of 1:5 and pulp the viscera into a pulp for later use. (5) Preparation of nutrient solution: Combine the above materials according to the ratio of 550 parts water, 300 parts visceral fluid, 90 parts tomatoes and 50 parts potatoes and grind them into a raw material sauce. Then add 8 parts brown sugar and 2 parts peptone to the raw material sauce and stir well to obtain the nutrient solution for later use. (6) Preparation of culture medium: Combine the above materials into auxiliary materials according to the ratio of 55 parts wheat, 15 parts ginseng, 10 parts polygonatum, 10 parts cistanche, 5 parts lotus root powder and 5 parts silkworm pupa powder. Then, according to the ratio of auxiliary materials to nutrient solution of 1:1.3, place the auxiliary materials and nutrient solution in a container. Then, sterilize the auxiliary materials and nutrient solution at a temperature of 121℃ and a pressure of 0.15 MPa for 25 minutes to obtain culture medium. (7) Preparation of culture medium: In a sterile environment, mix the liquid inoculum of Cordyceps militaris into the culture medium according to the ratio of 10 parts Cordyceps militaris liquid bacteria to culture medium of 100 parts. Then, stir evenly to obtain culture medium for later use.

[0063] The steps for dispensing the culture medium into the turtle shell are as follows: (1) Covering the turtle with fungal culture: Spray the entire turtle body with Cordyceps militaris liquid culture under sterile conditions; (2) Dispensing the substrate into the turtle body: Dispense the culture medium mixed with Cordyceps militaris liquid culture into the turtle body through the venting holes set on the back of the turtle body under sterile conditions, so that the culture medium fills the entire turtle body, and the culture medium in the turtle body is level with the venting holes, and the culture medium in the turtle body is compact and does not form excessive compression.

[0064] The aforementioned cultivation device comprises an incubator, a cultivation rack, LED strip lights, an ozone generator, an ultrasonic humidifier, an exhaust fan, a humidity control instrument, a time relay, a time control switch, and an air humidity regulating instrument. The cultivation rack is placed inside the incubator; the ultrasonic humidifier is placed at the bottom of the incubator; the LED strip lights are placed on the inner walls of the incubator on the front, back, left, and right sides; the oxygen outlet pipe of the ozone generator is connected to the inside of the incubator; and the exhaust fan is installed at the top of the incubator. The humidity control instrument controls the operation of the ultrasonic humidifier; the time relay controls the on / off state of the LED strip lights; the time control switch controls the operation of the ozone generator; and the air humidity regulating instrument controls the operation of the exhaust fan.

[0065] The cultivation process of the Cordyceps militaris complex in the above-mentioned cultivation device is as follows: (1) Device disinfection: Manually start the time control switch to run the ozone generator and disinfect the inside of the cultivation box for 20 minutes; (2) Dark light cultivation: After the inside of the cultivation box is disinfected, place the turtle containing the culture medium into the turtle or tortoise body placement rack provided in the cultivation rack, and then carry out dark light cultivation until the Cordyceps militaris mycelium fills the culture medium in the turtle body and even the entire turtle body is covered with mycelium; (3) Light cultivation: Intelligent start time relay, turn on every 12 hours. To meet the requirement of the LED strip lighting for 10 hours, the corresponding parameters are set in the time relay so that the time relay automatically closes or opens every 12 hours, so that the LED strip lights up for 10 hours and then automatically turns off, so that the turtle is evenly and reasonably exposed to light until all the mycelium on the surface of the turtle changes color; (4) Scratching: 2-3 days after the mycelium changes color, scratch the mycelium to induce the germination of fruiting bodies; (5) Water filling and disinfection of the device: Pour clean water into the bottom of the incubator so that the water level submerges the ultrasonic atomizing humidifier, and then start the time control switch intelligently according to each room To meet the requirement of turning on the ozone generator every 24 hours for 2 minutes and then automatically turning it off, set the corresponding parameters on the time control switch. The time control switch will automatically perform the opening and closing operations of the ozone generator, so that the ozone can scientifically and reasonably disinfect the clean water injected at the bottom of the incubator; (6) Fruiting body cultivation: When the culture medium on the turtle body begins to germinate fruiting bodies, according to the requirement of turning on the light strip every 12 hours, turning on for 12 hours and turning off for 12 hours a day, set the corresponding parameters on the time relay, so that the time relay can effectively control the light strip according to the set opening and closing time; set the humidity control parameter of the humidity control instrument to 80%, so that the humidity control instrument can effectively control the ultrasonic atomizing humidifier according to the set humidity control parameter, so that the humidity environment in the incubator meets the requirements; according to the requirement that the ozone generator is turned on for 2 minutes and turned off for 24 hours, set the opening and closing time parameters on the time control switch, so that the time control switch can effectively and reasonably control the ozone generator according to the set parameters, and use ozone disinfection to keep the inside of the incubator clean to meet the requirements;The humidity control parameter of the air humidity regulator is set to 85%. This allows the air humidity regulator to effectively control the exhaust fan according to the set humidity control parameter. This function addresses the issue that the air humidity inside the incubator is obtained by controlling the operation of the ultrasonic atomizing humidifier through the humidity control instrument. When the ultrasonic atomizing humidifier controlled by the humidity control instrument is running, it brings the air humidity inside the incubator to the manually set value. Although the humidity control instrument has stopped the operation of the ultrasonic atomizing humidifier, because the incubator is closed and there is water placed at the bottom, the air humidity in the incubator often continues to rise. For example, the manually set air humidity is 80%, but the air humidity in the incubator will continue to rise to 85% or higher and will not drop for a long time. Once the humidity in the incubator is too high, it will also affect the normal growth of Cordyceps militaris. Therefore, it is very necessary to restore the humidity in the incubator to the manually set value as soon as possible. By using the air humidity regulator to control the operation of the exhaust fan, the exhaust fan will remove the excessive humidity in the incubator, thereby bringing the humidity environment inside the incubator to an ideal value, which is more conducive to the growth of Cordyceps militaris and achieves automated and precise control. Through intermittent ozone disinfection and proper control of light and humidity, a turtle-Cordyceps militaris complex was obtained by allowing Cordyceps militaris fruiting bodies to grow on its body.

[0066] The turtle and tortoise pupa cordyceps complex is soaked in baijiu (Chinese white liquor) to obtain turtle and tortoise pupa cordyceps wine products. The soaking steps are as follows: remove the mycelium and mycelium from the turtle head, feet, and tail of the turtle and tortoise pupa cordyceps complex and set aside; then place the processed turtle and tortoise pupa cordyceps complex in a liquor bottle, and then pour 33-degree baijiu into the bottle to soak the turtle and tortoise pupa cordyceps wine products for more than 30 days.

[0067] Example 3

[0068] The following method can be used to cultivate Cordyceps militaris using turtles as a parasite. The turtle body is used as a carrier, food is used as an auxiliary material, and Cordyceps militaris inoculum is used as the inoculum source. First, the fresh and intact turtle body is processed to remove the internal organs and fat. Then, the internal organs are degreased and deodorized, and combined with the auxiliary materials and sterilized to obtain the culture medium. Then, the Cordyceps militaris inoculum and the culture medium are combined to form a culture substrate. The culture substrate is then divided into turtle shells and placed in a culture device for cultivation. The turtle or tortoise shell is cultivated until it is covered with Cordyceps militaris fruiting bodies to obtain a turtle or tortoise Cordyceps militaris complex.

[0069] The above steps for processing fresh and intact turtle bodies are as follows: Select fresh and intact turtle bodies with a turtle age of 20 years, and then, according to the size of the turtle body, make 5-10 venting holes with a diameter of 20-40 mm and ventilation holes with a diameter of 3-5 mm on the back of the turtle body, and make 5-10 ventilation holes with a diameter of 3-5 mm on the abdomen of the turtle body, and then remove the turtle's internal organs and fat from the venting holes for later use; (2) Fat removal treatment: Place the processed turtle body in a cooking container, fill it with water to submerge the turtle body, then heat it until the water boils, and continue to cook for 10 minutes, using boiling water Remove the fat remaining in the turtle body for later use; (3) According to the ratio of 10 kg of water and 1.5 kg of star anise, put the water and star anise together in a cooking container, then heat until the water in the container boils, and continue heating for 10 minutes to obtain star anise solution. Then soak the turtle body that has been treated to remove fat in the star anise solution for 30 minutes, and use the aroma of star anise to eliminate the fishy smell of the turtle. Finally, take it out and dry it for later use; (4) Place the turtle body that has been treated to remove the fishy smell in a high pressure sterilizer and sterilize it for 25 minutes at a temperature of 121℃ and a pressure of 0.15 MPa.

[0070] The preparation process of the above culture medium is as follows: (1) Wash the collected turtle viscera with clean water to remove blood, and then remove the gallbladder and the fat adhering to the viscera for later use; (2) Fat removal treatment of viscera: Place the viscera in a cooking container, add water to submerge the viscera, then heat until the water boils and continue to cook for 10 minutes to remove the fat remaining in the viscera; (3) Intervention of fishy smell of viscera: According to the ratio of 10 kg of water and 1 kg of star anise, place the water and star anise together in a cooking container, then heat until the water in the container boils and continue to heat for 10 minutes to obtain star anise solution, then place the fat-removed viscera in the star anise solution and soak for 20 minutes to remove the fishy smell of the viscera with the aroma of star anise; (4) Viscera pulping: According to the ratio of viscera and water of 1:5, place the viscera and water together in a pulping machine to pulp the viscera for later use; (5) Preparation of nutrient solution: According to 500 parts (6) Preparation of culture medium: Combine the above materials into a raw material sauce according to the ratio of water, 350 parts of visceral fluid, 85 parts of tomatoes, and 45 parts of potatoes. Then add 9 parts of honey, 8 parts of brown sugar, and 3 parts of peptone to the raw material sauce and stir evenly to obtain a nutrient solution for later use; (7) Preparation of culture medium: Combine the above materials into an auxiliary material according to the ratio of 49 parts of wheat, 12 parts of tremella, 10 parts of donkey-hide gelatin, 8 parts of codonopsis, 6 parts of red dates, 5 parts of wolfberry, 5 parts of star anise seed bran, and 5 parts of silkworm pupa powder. Then place the auxiliary material and nutrient solution in a container according to the ratio of 1:1.3. Then sterilize the auxiliary material and nutrient solution at a temperature of 121℃ and a pressure of 0.15 MPa for 25 minutes to obtain a culture medium; (8) Preparation of culture medium: In a sterile environment, mix the Cordyceps militaris liquid bacteria into the culture medium according to the ratio of 10 parts:100 parts. Then stir evenly to obtain a culture medium for later use.

[0071] The steps for dispensing the culture medium into the turtle shell are as follows: (1) Covering the turtle with fungal culture: Spray the entire turtle body with Cordyceps militaris liquid culture under sterile conditions; (2) Dispensing the substrate into the turtle body: Dispense the culture medium mixed with Cordyceps militaris liquid culture into the turtle body through the venting holes set on the back of the turtle body under sterile conditions, so that the culture medium fills the entire turtle body, and the culture medium in the turtle body is level with the venting holes, and the culture medium in the turtle body is compact and does not form excessive compression.

[0072] The aforementioned cultivation device comprises an incubator, a cultivation rack, LED strip lights, an ozone generator, an ultrasonic humidifier, an exhaust fan, a humidity control instrument, a time relay, a time control switch, and an air humidity regulating instrument. The cultivation rack is placed inside the incubator; the ultrasonic humidifier is placed at the bottom of the incubator; the LED strip lights are placed on the inner walls of the incubator on the front, back, left, and right sides; the oxygen outlet pipe of the ozone generator is connected to the inside of the incubator; and the exhaust fan is installed at the top of the incubator. The humidity control instrument controls the operation of the ultrasonic humidifier; the time relay controls the on / off state of the LED strip lights; the time control switch controls the operation of the ozone generator; and the air humidity regulating instrument controls the operation of the exhaust fan.

[0073] The cultivation process of the Cordyceps militaris complex in the above-mentioned cultivation device is as follows: (1) Device disinfection: Manually start the time control switch to run the ozone generator and disinfect the inside of the cultivation box for 20 minutes; (2) Dark light cultivation: After the inside of the cultivation box is disinfected, place the turtle containing the culture medium into the turtle or tortoise body placement rack provided in the cultivation rack, and then carry out dark light cultivation until the Cordyceps militaris mycelium fills the culture medium in the turtle body and even the entire turtle body is covered with mycelium; (3) Light cultivation: Intelligent start time relay, turn on every 12 hours. To meet the requirement of the LED strip lighting for 10 hours, the corresponding parameters are set in the time relay so that the time relay automatically closes or opens every 12 hours, so that the LED strip lights up for 10 hours and then automatically turns off, so that the turtle is evenly and reasonably exposed to light until all the mycelium on the surface of the turtle changes color; (4) Scratching: 2-3 days after the mycelium changes color, scratch the mycelium to induce the germination of fruiting bodies; (5) Water filling and disinfection of the device: Pour clean water into the bottom of the incubator so that the water level submerges the ultrasonic atomizing humidifier, and then start the time control switch intelligently according to each room To meet the requirement of turning on the ozone generator every 24 hours for 2 minutes and then automatically turning it off, set the corresponding parameters on the time control switch. The time control switch will automatically perform the opening and closing operations of the ozone generator, so that the ozone can scientifically and reasonably disinfect the clean water injected at the bottom of the incubator; (6) Fruiting body cultivation: When the culture medium on the turtle body begins to germinate fruiting bodies, according to the requirement of turning on the light strip every 12 hours, turning on for 12 hours and turning off for 12 hours a day, set the corresponding parameters on the time relay, so that the time relay can effectively control the light strip according to the set opening and closing time; set the humidity control parameter of the humidity control instrument to 80%, so that the humidity control instrument can effectively control the ultrasonic atomizing humidifier according to the set humidity control parameter, so that the humidity environment in the incubator meets the requirements; according to the requirement that the ozone generator is turned on for 2 minutes and turned off for 24 hours, set the opening and closing time parameters on the time control switch, so that the time control switch can effectively and reasonably control the ozone generator according to the set parameters, and use ozone disinfection to keep the inside of the incubator clean to meet the requirements;The humidity control parameter of the air humidity regulator is set to 85%. This allows the air humidity regulator to effectively control the exhaust fan according to the set humidity control parameter. This function addresses the issue that the air humidity inside the incubator is obtained by controlling the operation of the ultrasonic atomizing humidifier through the humidity control instrument. When the ultrasonic atomizing humidifier controlled by the humidity control instrument is running, it brings the air humidity inside the incubator to the manually set value. Although the humidity control instrument has stopped the operation of the ultrasonic atomizing humidifier, because the incubator is closed and there is water placed at the bottom, the air humidity in the incubator often continues to rise. For example, the manually set air humidity is 80%, but the air humidity in the incubator will continue to rise to 85% or higher and will not drop for a long time. Once the humidity in the incubator is too high, it will also affect the normal growth of Cordyceps militaris. Therefore, it is very necessary to restore the humidity in the incubator to the manually set value as soon as possible. By using the air humidity regulator to control the operation of the exhaust fan, the exhaust fan will remove the excessive humidity in the incubator, thereby bringing the humidity environment inside the incubator to an ideal value, which is more conducive to the growth of Cordyceps militaris and achieves automated and precise control. Through intermittent ozone disinfection and proper control of light and humidity, a turtle-Cordyceps militaris complex was obtained by allowing Cordyceps militaris fruiting bodies to grow on its body.

[0074] The turtle and tortoise pupa cordyceps complex is soaked in baijiu (Chinese white liquor) to obtain turtle and tortoise pupa cordyceps wine products. The soaking steps are as follows: remove the mycelium and mycelium from the turtle head, feet, and tail of the turtle and tortoise pupa cordyceps complex and set aside; then place the processed turtle and tortoise pupa cordyceps complex in a liquor bottle, and then pour 45-degree baijiu into the bottle to soak the turtle and tortoise pupa cordyceps wine products for more than 30 days.

[0075] Example 4

[0076] The following method can be used to cultivate Cordyceps militaris using turtles as a parasite. It uses turtles as carriers, food as supplementary materials, and Cordyceps militaris spores as the inoculum. First, the fresh and intact turtle body is processed to remove the internal organs and fat. Then, the internal organs are degreased and deodorized before being combined with the supplementary materials and sterilized to obtain the culture medium. Then, the Cordyceps militaris spores and the culture medium are combined to form a culture substrate. The culture substrate is then dispensed into the turtle shell and placed in a culture device for cultivation. The turtle is cultivated until the shell is covered with Cordyceps militaris fruiting bodies to obtain the turtle-Cordyceps militaris complex.

[0077] The above steps for processing fresh and complete turtle bodies are as follows: (1) Select fresh and complete turtle bodies with a turtle age of 10 years, place them in a refrigeration device and refrigerate them until the turtle bodies are frozen and hardened. Then drill 7 venting holes and venting holes on the back and 3 venting holes on the abdomen. Then remove the internal organs and fat from the venting holes. (2) Place the turtle shell with the internal organs and fat removed in a cooking container, add water to submerge the turtle shell, then heat until the water boils and continue to cook for 10 minutes to remove the fat remaining in the turtle body. (3) According to the ratio of 10 kg of water and 1 kg of star anise, put the water and star anise together in a cooking container, then heat until the water in the container boils and continue to heat for 10 minutes to obtain a star anise solution. Then soak the turtle shell that has been degreased in the star anise solution for 30 minutes. (4) Place the turtle shell from the previous step into a high-temperature sterilizer and sterilize it for 22 minutes at a temperature of 121°C and a pressure of 0.15 MPa.

[0078] The preparation process of the above-mentioned culture medium is as follows: (1) Clean the internal organs of the turtle to remove blood, and then remove the gallbladder and the fat adhering to the internal organs for later use. (2) Place the internal organs in a cooking container, add water to submerge the internal organs, then heat until the water boils and continue to cook for 10 minutes to remove the fat remaining in the internal organs for later use. (3) According to the ratio of 10 kg of water to 1 kg of star anise, place the water and star anise together in a cooking container, then heat until the water in the container boils and continue to heat for 10 minutes to obtain a star anise solution, and then place the degreased internal organs in the star anise solution to soak for 30 minutes for later use. (4) According to the weight ratio of internal organs to water of 1:5, place the internal organs and water together in a pulping machine to pulp the internal organs for later use. (5) Combine the above materials in the ratio of 550 parts water, 300 parts visceral fluid, 90 parts tomatoes, and 50 parts potatoes and grind them into a raw material sauce. Then add 8 parts brown sugar and 2 parts peptone to the raw material sauce and stir well to obtain a nutrient solution for later use. (6) Combine the above materials in the ratio of 85 parts rice, 10 parts star anise seed bran, and 5 parts silkworm pupa powder to form an auxiliary material. Then place the auxiliary material and nutrient solution in a container in a ratio of 1:1.3. Then sterilize the auxiliary material and nutrient solution at a temperature of 121℃ and a pressure of 0.15 MPa for 25 minutes to obtain a culture medium. (7) In a sterile environment, mix the Cordyceps militaris liquid inoculum into the culture medium in a ratio of 5 parts:100 parts and stir well to obtain a culture medium. The above ratios are weight fractions.

[0079] The steps for dispensing the culture medium into the turtle shell are as follows: (1) Under sterile conditions, spray the liquid Cordyceps militaris inoculum evenly over the entire turtle shell. (2) Under sterile conditions, dispense the culture medium containing the liquid Cordyceps militaris inoculum into the turtle shell through the inoculum outlets set on the turtle shell.

[0080] The aforementioned cultivation device comprises an incubator, a cultivation rack, LED strip lights, an ozone generator, an ultrasonic humidifier, an exhaust fan, a humidity control instrument, a time relay, a time control switch, and an air humidity regulating instrument. The cultivation rack is placed inside the incubator; the ultrasonic humidifier is placed at the bottom of the incubator; the LED strip lights are placed on the inner walls of the incubator on the front, back, left, and right sides; the oxygen outlet pipe of the ozone generator is connected to the inside of the incubator; and the exhaust fan is installed at the top of the incubator. The humidity control instrument controls the operation of the ultrasonic humidifier; the time relay controls the on / off state of the LED strip lights; the time control switch controls the operation of the ozone generator; and the air humidity regulating instrument controls the operation of the exhaust fan.

[0081] The cultivation process of the Cordyceps militaris complex in the above-mentioned cultivation device is as follows: (1) Device disinfection: Start the time control switch to run the ozone generator and disinfect the inside of the cultivation box. (2) Dark light cultivation: Place the turtle shell containing the culture medium on the cultivation rack, and then carry out dark light cultivation until the Cordyceps militaris mycelium fills the culture medium inside the turtle shell and even the entire turtle shell is covered with mycelium. (3) Light cultivation: Intelligent start time relay, according to the requirement of turning on the light strip for 10 hours every 12 hours, set the corresponding parameters in the time relay, so that the time relay automatically closes or opens every 12 hours, so that the light strip turns on for 10 hours and then automatically closes, so that the turtle is evenly and reasonably exposed to light until all the mycelium on the surface of the turtle changes color. (4) Scratching: 2-3 days after the mycelium changes color, scratch the mycelium to induce the germination of fruiting bodies. (5) Water filling and disinfection of the device: Fill the bottom of the incubator with clean water so that the water level submerges the ultrasonic atomizing humidifier. Then, start the time control switch intelligently. According to the requirement that the ozone generator is turned on for 2 minutes every 24 hours and then automatically turned off, set the corresponding parameters on the time control switch. The time control switch will automatically perform the on and off operation of the ozone generator so that the ozone can scientifically and reasonably disinfect the clean water injected at the bottom of the incubator (1).(6) Fruiting body cultivation: When the culture medium on the turtle begins to germinate fruiting bodies, according to the requirement of turning on the light strip every 12 hours, turning it on for 12 hours and off for 12 hours a day, set the corresponding parameters on the time relay so that the time relay can effectively control the light strip according to the set on and off times; set the humidity control parameter of the humidity control instrument to 80% so that the humidity control instrument can effectively control the ultrasonic atomizing humidifier according to the set humidity control parameter so that the humidity environment in the incubator meets the requirements; according to the requirement that the ozone generator is turned on for 2 minutes and turned off for 24 hours, set the on and off time parameters on the time control switch so that the time control switch can effectively and reasonably control the ozone generator according to the set parameters, and use ozone disinfection to keep the inside of the incubator clean to meet the requirements; set the humidity control parameter of the air humidity control instrument to 85% so that the air humidity control instrument can effectively control the exhaust fan according to the set humidity control parameter. The humidity inside the incubator is controlled by a humidity control instrument that operates an ultrasonic atomizing humidifier. When the ultrasonic atomizing humidifier reaches the preset humidity level, even after the humidity control instrument has stopped operating, the humidity level often continues to rise due to the enclosed nature of the incubator and the presence of water at the bottom. For example, if the preset humidity is 80%, the humidity may rise to 85% or higher and remain high for a long time. Excessive humidity can negatively impact the growth of Cordyceps militaris. Therefore, it is crucial to restore the humidity to the preset value as quickly as possible. Using a humidity control instrument to operate an exhaust fan removes excess humidity from the incubator, achieving an ideal humidity level that promotes Cordyceps militaris growth and allows for automated and precise control. Through intermittent ozone disinfection and proper control of light and humidity, a Cordyceps militaris complex is obtained by allowing Cordyceps militaris fruiting bodies to grow on the turtle's body.

[0082] The aforementioned Cordyceps militaris complex is soaked in baijiu (Chinese liquor) to obtain Cordyceps militaris liquor products. The soaking steps are as follows: remove the mycelium and mycelium from the head, feet, and tail of the Cordyceps militaris complex and set aside; then place the processed Cordyceps militaris complex in a liquor bottle, and then pour 53-degree baijiu into the bottle to soak the Cordyceps militaris liquor products for more than 30 days.

[0083] Example 5

[0084] The following method can be used to cultivate Cordyceps militaris using turtles as a parasite. It uses turtles as carriers, food as supplementary materials, and Cordyceps militaris spores as the inoculum. First, the fresh and intact turtle body is processed to remove the internal organs and fat. Then, the internal organs are degreased and deodorized before being combined with the supplementary materials and sterilized to obtain the culture medium. Then, the Cordyceps militaris spores and the culture medium are combined to form a culture substrate. The culture substrate is then dispensed into the turtle shell and placed in a culture device for cultivation. The turtle is cultivated until the shell is covered with Cordyceps militaris fruiting bodies to obtain the turtle-Cordyceps militaris complex.

[0085] The above steps for processing fresh and intact turtle bodies are as follows: (1) Select fresh and intact turtle bodies with a turtle age of 10 years, place them in a refrigeration device and refrigerate them until the turtle bodies are frozen and hardened. Then drill 7 venting holes and venting holes on the back and 3 venting holes on the abdomen. Then remove the internal organs and fat from the venting holes. (2) Place the turtle shell with the internal organs and fat removed in a cooking container, fill it with water to submerge the turtle shell, heat it until the water boils, and continue to cook for 10 minutes to remove the fat remaining in the turtle body for later use. (3) Place the water and star anise together in a cooking container according to the ratio of 10 kg water and 1 kg star anise. Then heat the water in the container until it boils and continue heating for 10 minutes to obtain a star anise solution. Then soak the turtle body that has been degreased in the star anise solution for 30 minutes to eliminate the fishy smell of the turtle by using the aroma of star anise. (4) Place the turtle body that has been treated to remove the fishy smell in a high-temperature sterilizer and sterilize it for 22 minutes at a temperature of 121°C and a pressure of 0.15 MPa.

[0086] The preparation process of the above-mentioned culture medium is as follows: (1) Treatment of turtle viscera: Clean the collected turtle viscera to remove blood, and then remove the gallbladder and the fat adhering to the viscera for later use; (2) Fat removal treatment of viscera: Place the viscera in a cooking container, add water to submerge the viscera, then heat until the water boils and continue to cook for 10 minutes. Use the heat of boiling water to dissolve the fat remaining in the viscera to achieve the purpose of eliminating fat; (3) Intervention of fishy smell of viscera: According to the ratio of 10 kg of water and 1 kg of star anise, place the water and star anise together in a cooking container, then heat until the water in the container boils and continue to heat for 10 minutes to obtain star anise solution. Then place the viscera that has been treated with fat removal in the star anise solution and soak for 30 minutes for later use; (4) Viscera pulping: According to the ratio of viscera and water of 1:5, place the viscera and water together in a pulping machine to pulp the viscera for later use; (5) Preparation of nutrient solution: According to the ratio of 520 parts water, 360 parts visceral slurry, 60 parts fresh lotus root, and 50 parts potato, the above materials are combined and blended into a raw material sauce. Then, 8 parts brown sugar and 2 parts peptone are added to the raw material sauce and stirred evenly to obtain a nutrient solution for later use. (6) Preparation of culture medium: According to the ratio of 35 parts rice, 25 parts soybeans, 15 parts lotus seeds, 10 parts white fungus, 10 parts star anise seed bran, and 5 parts silkworm pupa powder, the above materials are combined into an auxiliary material. Then, according to the ratio of auxiliary material to nutrient solution of 1:1.3, the auxiliary material and nutrient solution are placed in a container. Then, the auxiliary material and nutrient solution are sterilized at a temperature of 121℃ and a pressure of 0.15 MPa for 25 minutes to obtain a culture medium. (7) Preparation of culture medium: In a sterile environment, according to the ratio of 5 parts Cordyceps militaris liquid bacteria to culture medium of 100 parts, Cordyceps militaris liquid bacteria are mixed into the culture medium and stirred evenly to obtain a culture medium for later use.

[0087] The above steps for dispensing the culture medium into the turtle shell are as follows: (1) Covering the turtle body with bacteria: spray the whole turtle body with Cordyceps militaris liquid bacteria under sterile conditions; (2) Dispensing the turtle body with the substrate: dispensing the culture medium mixed with Cordyceps militaris liquid bacteria into the turtle body under sterile conditions through the bacteria outlet positions set on the back of the turtle body for later use.

[0088] The aforementioned cultivation device comprises an incubator, a cultivation rack, LED strip lights, an ozone generator, an ultrasonic humidifier, an exhaust fan, a humidity control instrument, a time relay, a time control switch, and an air humidity regulating instrument. The cultivation rack is placed inside the incubator; the ultrasonic humidifier is placed at the bottom of the incubator; the LED strip lights are placed on the inner walls of the incubator on the front, back, left, and right sides; the oxygen outlet pipe of the ozone generator is connected to the inside of the incubator; and the exhaust fan is installed at the top of the incubator. The humidity control instrument controls the operation of the ultrasonic humidifier; the time relay controls the on / off state of the LED strip lights; the time control switch controls the operation of the ozone generator; and the air humidity regulating instrument controls the operation of the exhaust fan.

[0089] The cultivation process of the Cordyceps militaris complex in the above-mentioned cultivation device is as follows: (1) Device disinfection: Manually start the time control switch to run the ozone generator and disinfect the inside of the incubator for 20 minutes; (2) Dark light cultivation: After the inside of the incubator is disinfected, place the turtle body containing the culture medium into the turtle body placement rack provided by the cultivation rack, and then carry out dark light cultivation until the Cordyceps militaris mycelium fills the culture medium in the turtle body and even the entire turtle body is covered with mycelium; (3) Light cultivation: Intelligent start time relay, turn on the light strip every 12 hours. To meet the 10-hour light requirement, set the corresponding parameters in the time relay so that the time relay automatically closes or opens every 12 hours, so that the light strip lights up for 10 hours and then automatically turns off, so that the turtle is evenly and reasonably exposed to light until all the mycelium on the surface of the turtle changes color; (4) Scratching: 2-3 days after the mycelium changes color, scratch the mycelium to induce the germination of fruiting bodies; (5) Water injection and disinfection of the device: inject clean water into the bottom of the incubator so that the water level submerges the ultrasonic atomizing humidifier, and then start the time control switch intelligently every 24 hours. To meet the requirement that the ozone generator automatically shuts off after running for 2 minutes every hour, the corresponding parameters are set on the time control switch. The time control switch automatically performs the ozone generator's on / off operation, so that the ozone can scientifically and reasonably disinfect the clean water injected into the bottom of the incubator (1). (6) Fruiting body cultivation: When the culture medium on the turtle's body begins to germinate fruiting bodies, the light strip is turned on every 12 hours, and the light is turned on for 12 hours and off for 12 hours every day. The corresponding parameters are set on the time relay, so that the time relay can effectively control the light strip according to the set on and off time. The humidity control parameter of the humidity control instrument is set to 80%, so that the humidity control instrument can effectively control the ultrasonic atomizing humidifier according to the set humidity control parameter, so that the humidity environment in the incubator meets the requirements. According to the requirement that the ozone generator is turned on for 2 minutes and turned off for 24 hours, the on and off time parameters are set on the time control switch, so that the time control switch can effectively and reasonably control the ozone generator according to the set parameters, and use ozone disinfection to keep the inside of the incubator clean to meet the requirements.The humidity control parameter of the air humidity regulator is set to 85%. This allows the air humidity regulator to effectively control the exhaust fan according to the set humidity control parameter. This function addresses the issue that the air humidity inside the incubator is obtained by controlling the operation of the ultrasonic atomizing humidifier through the humidity control instrument. When the ultrasonic atomizing humidifier controlled by the humidity control instrument is running, it brings the air humidity inside the incubator to the manually set value. Although the humidity control instrument has stopped the operation of the ultrasonic atomizing humidifier, because the incubator is closed and there is water placed at the bottom, the air humidity in the incubator often continues to rise. For example, the manually set air humidity is 80%, but the air humidity in the incubator will continue to rise to 85% or higher and will not drop for a long time. Once the humidity in the incubator is too high, it will also affect the normal growth of Cordyceps militaris. Therefore, it is very necessary to restore the humidity in the incubator to the manually set value as soon as possible. By using the air humidity regulator to control the operation of the exhaust fan, the exhaust fan will remove the excessive humidity in the incubator, thereby bringing the humidity environment inside the incubator to an ideal value, which is more conducive to the growth of Cordyceps militaris and achieves automated and precise control. Through intermittent ozone disinfection and proper control of light and humidity, a turtle-Cordyceps militaris complex was obtained by allowing Cordyceps militaris fruiting bodies to grow on its body.

[0090] The aforementioned Cordyceps militaris complex is soaked in baijiu (Chinese liquor) to obtain Cordyceps militaris liquor products. The soaking steps are as follows: remove the mycelium and mycelium from the head, feet, and tail of the Cordyceps militaris complex and set aside; then place the processed Cordyceps militaris complex in a liquor bottle, and then pour 55-degree baijiu into the bottle to soak the Cordyceps militaris liquor products for more than 30 days.

[0091] In the process of cultivating Cordyceps militaris and producing its wine products using turtles as parasites in Example 1, a control group was simultaneously implemented: a nutrient solution made of 550 parts water, 300 parts turtle visceral fluid, 90 parts tomatoes, 70 parts potatoes, 8 parts brown sugar, and 2 parts peptone, and a culture medium composed of 85 parts wheat, 10 parts star anise seed bran, and 5 parts silkworm pupa powder were used to cultivate Cordyceps militaris using conventional methods. Then, turtles of the same age and weight as those in Example 1 were collected, dried, and placed in the same amount of white wine as those in Example 1 for 30 days to produce a Cordyceps militaris wine.

[0092] A comparison table of turtle collection, substrate formulation, cultivation, and wine production for the two schemes (Table 1)

[0093] Comparison of component test results of wine products prepared by the two methods (Table 2)

[0094] As shown in Table 1 above, the turtle age, weight, and treatment methods of the turtles collected in the control group and Example 1 were basically the same, and the nutrient solution formula and substrate formula were completely the same. The key difference was that the cultivation container used in Example 1 was a turtle body, while the cultivation container used in the control group was a plastic box. In Example 1, the inoculation method was to directly mix the Cordyceps militaris liquid culture medium and the culture medium evenly, while in the control group, the liquid culture medium was sprayed onto the substrate surface. In the process of soaking in alcohol, in Example 1, the whole turtle Cordyceps militaris complex was placed in the liquor for soaking, while in the control group, the turtle body was dried and the freshly harvested Cordyceps militaris was placed in the liquor for soaking. Regarding mycelial growth, in Example 1, all mycelia germinated in the substrate after 24 hours and completely covered the substrate by day 3; in the control group, mycelia germinated on the surface of the substrate after 24 hours and completely covered the substrate by day 10. Regarding color change and fruiting body germination, in Example 1, color change occurred on day 4, mycelia were scratched on day 8, and fruiting bodies germinated on day 12; in the control group, color change occurred on day 11, mycelia were scratched on day 15, and fruiting bodies germinated on day 19. The fruiting body maturation period in Example 1 was 52 days, while in the control group it was 59 days. Regarding bioconversion rate, in Example 1, the dry weight of the culture medium was 100 grams, and the harvested fresh Cordyceps fruiting bodies were 109 grams, resulting in a bioconversion rate of 109%; in the control group, the dry weight of the culture medium was 100 grams, and the harvested fresh Cordyceps fruiting bodies were 112 grams, resulting in a bioconversion rate of 112%. It is evident that the two technical schemes, with essentially the same turtle body collection and treatment, nutrient solution formulation, and substrate formulation, yielded essentially the same bioconversion rate. However, due to differences in cultivation containers and inoculation methods, there are significant differences in mycelial growth, color change, fruiting body germination, and fruiting body maturity between the two technical schemes. In Example 1, the mycelium fully colonized the substrate by day 3, and the fruiting body germination and maturity were completed 7 days earlier than the control group. The formation of the wine products also differs significantly between the two technical schemes. In Example 1, Cordyceps militaris grew on the turtle's body, and the turtle retained its original shape under the intertwining of mycelium, forming a novel, unique, and beautiful Cordyceps militaris composite. When placed in a wine bottle, this composite presented a wine with a golden color, novel and unique shape, rich nutrition, and an eye-catching appearance. In contrast, in the control group, the turtle's body in the bottle was incomplete, the Cordyceps militaris was irregularly distributed, and the overall product was disorganized, resulting in a wine with poor commercial performance. Therefore, Example 1, by innovatively using the turtle as a cultivation container and changing the inoculation mode, achieved rapid mycelial growth, a short fruiting body cultivation time, and a highly distinctive Cordyceps militaris composite. The final wine product also demonstrated superior quality and a strong market advantage.

[0095] The wine products prepared using both methods were commissioned to a qualified third-party testing institution for testing. The test results are shown in Table 2. Table 2 shows that the tested items included cordycepin, adenosine, crude polysaccharide, total flavonoids (calculated as rutin), protein (Nx6.25), and total hydrolyzed amino acids. The Cordyceps militaris wine provided in Example 1 contained 432 mg / kg cordycepin, 24.5 mg / kg adenosine, 0.14% crude polysaccharide, 21.9 mg / 100g total flavonoids (calculated as rutin), 2.16 g / 100g protein (Nx6.25), and 1.27 g / 100g total hydrolyzed amino acids. The Cordyceps militaris wine provided in the control group contained 177 mg / kg cordycepin, 35.0 mg / kg adenosine, 0.77 g / 100g protein (Nx6.25), 0.056% crude polysaccharide, 21.8 mg / 100g total flavonoids (calculated as rutin), and 0.38 g / 100g total hydrolyzed amino acids. It is evident that, except for the adenosine content of the Cordyceps militaris wine provided in the control group being higher than that in Example 1, the other tested indicators in the control group were significantly lower than those in the Cordyceps militaris wine provided in Example 1. In particular, the core component cordycepin was more than twice as high in Example 1 as in the control group, the protein content in Example 1 was nearly three times higher, and the total hydrolyzed amino acid content in Example 1 was more than three times higher. Therefore, Example 1 innovatively used a turtle as a culture container, allowing Cordyceps militaris mycelia and fruiting bodies to grow on turtles or tortoises. The turtle's nutrients were used for the absorption of the Cordyceps militaris fruiting bodies, resulting in a significantly higher level of cordycepin compared to the control group. During the germination process on the turtle's body, the mycelia simultaneously penetrated the turtle's tissues, effectively decomposing the turtle itself from large molecules into smaller ones. After soaking in high-proof alcohol, not only was a bottle of wine with excellent color, aroma, taste, nutrition, and aesthetic appeal formed, but the high-proof alcohol also quickly extracted the effective components from the turtle and dissolved them in the alcohol. The extracted protein, total hydrolyzed amino acid content, and other components were also significantly higher than those in the control group. When people drink alcohol, it's equivalent to absorbing the effective components of tortoise shell and cordyceps, resulting in better health benefits.

[0096] To verify the safety performance of the Cordyceps militaris wine obtained in the examples, an acute toxicity test was conducted on the Cordyceps militaris wine by a third party with food testing qualifications, and the following experimental results were obtained: 1. Experimental Results No toxic reactions or deaths were observed in mice after administration of the concentrated Cordyceps militaris wine extract. During the experiment, the mice grew well, moved normally, and had good coat luster. At the end of the experiment, the mice were euthanized and gross necropsies were performed; no abnormalities were found in any organs. The oral MTD of the concentrated Cordyceps militaris wine extract in mice was calculated to be >257.2 g of medicinal material per kg⁻¹, equivalent to 1512 times the human daily dose (0.17 g of medicinal material per kg⁻¹). The results are shown in Table 3.

[0097]

[0098] 2. Determination of the maximum tolerable dose of Cordyceps militaris wine Twenty healthy mice, weighing 18–22 g (half male and half female), were used. Before gavage, the mice were fasted for 16 hours but allowed free access to water. The test substance, *Cordyceps militaris* and *Gnaphalium affine* wine (10 ml of concentrated *Cordyceps militaris* and *Gnaphalium affine* wine plus 20 ml of base liquor (48% alcohol content)), was administered via gavage three times within 24 hours. After gavage administration, the mice were allowed free access to food and water. The animals' weight, signs of poisoning, and mortality were observed and recorded over 14 days.

[0099] Experimental Results: 10–15 minutes after administration of the Cordyceps militaris and Tortoise Shell Wine test substance, mice gradually exhibited symptoms such as excitement, unsteady gait, and drowsiness. These symptoms returned to normal after 40–50 minutes, indicating clear signs of intoxication. No other obvious toxic reactions were observed, and no animals died. During the experiment, the mice grew well, moved normally, and had good coat gloss. At the end of the experiment, the mice were euthanized, and a gross anatomical examination revealed no abnormalities in any organs. Calculations showed that the oral MTD of the Cordyceps militaris and Tortoise Shell Wine test substance in mice was >128.4 g of medicinal material per kg⁻¹, equivalent to 755 times the human daily dose (0.17 g of medicinal material per kg⁻¹). The results are shown in Table 4.

[0100]

[0101] 3. Conclusion The oral MTD of Cordyceps militaris and Cordyceps sinensis wine concentrate in mice is >257.2 g·kg⁻¹, which is equivalent to 1512 times the daily human dose; the oral MTD of Cordyceps militaris and Cordyceps sinensis wine in mice is >128.4 g·kg⁻¹, which is equivalent to 755 times the daily human dose. It is generally believed that, based on body weight, the maximum tolerated dose is more than 100 times the daily adult dose to be safe.

[0102] The above description is not intended to limit the present invention, nor is the present invention limited to the above examples. Any changes, modifications, additions or substitutions made by those skilled in the art within the scope of the present invention should be protected by the present invention.

Claims

1. A method for cultivating Cordyceps militaris using turtles and tortoises as parasites, characterized in that, This method uses turtles or tortoises as carriers, food ingredients as supplementary ingredients, and Cordyceps militaris spawn as the inoculum source. First, the fresh and intact turtle or tortoise body is processed to remove the internal organs and fat. Then, the internal organs are degreased and deodorized, combined with the supplementary ingredients, and sterilized to obtain a culture medium. Then, the Cordyceps militaris spawn and the culture medium are combined to form a culture substrate. The culture substrate is then divided into turtle or tortoise shells and placed in a culture device for cultivation. The turtle or tortoise is cultivated until the shells are covered with Cordyceps militaris fruiting bodies to obtain a turtle or tortoise Cordyceps militaris complex. The steps for processing a fresh, intact turtle or tortoise carcass are as follows: (1) Select a fresh and complete turtle or tortoise body, then drill several fungus outlets and ventilation holes on the back and several ventilation holes on the abdomen. Then remove the internal organs and fat from the fungus outlets. Before drilling the fungus outlets and ventilation holes, place the tortoise body in a refrigeration device and freeze it until the tortoise body is frozen and hardened. Then drill the fungus outlets and ventilation holes on the back and abdomen of the tortoise body. (2) Place the turtle or tortoise shell with its internal organs and fat removed into a cooking container, add water to submerge the turtle or tortoise shell, then heat until the water boils and continue cooking for 5-15 minutes to remove the fat remaining in the turtle or tortoise body for later use. (3) According to the ratio of 10 kg of water and 0.5-1 kg of star anise, put the water and star anise together in a cooking container, then heat until the water in the container boils, and continue heating for 5-10 minutes to obtain star anise solution. Then soak the turtle or tortoise shell that has been degreased in the star anise solution for 10-30 minutes for later use. (4) Place the turtle or tortoise shell from the previous step into a sterilization device for sterilization. The preparation process of the culture medium is as follows: (1) Clean the internal organs of the turtle or tortoise to remove the blood, and then remove the gallbladder and the fat adhering to the internal organs for later use. (2) Place the offal in a cooking container, add water to submerge the offal, then heat until the water boils and continue cooking for 5-10 minutes to remove the remaining fat from the offal for later use. (3) According to the ratio of 10 kg of water and 0.5-1 kg of star anise, put the water and star anise into a cooking container, then heat until the water in the container boils, and continue heating for 5-10 minutes to obtain star anise solution. Then soak the viscera that have been degreased in the star anise solution for 10-30 minutes for later use. (4) According to the weight ratio of viscera to water of 1:3-5, put the viscera and water together in a pulping machine and pulp them into a paste for later use. (5) Combine the above materials and blend them into a raw material sauce according to the ratio of 400-600 parts water, 200-400 parts visceral slurry, 90-100 parts tomatoes and 40-60 parts potatoes. Then add 6-10 parts brown sugar and 1-3 parts peptone to the raw material sauce and stir well to obtain a nutrient solution for later use. (6) Combine the above materials into auxiliary materials according to the ratio of 80-90 parts of ingredients, 8-12 parts of star anise seed bran, and 3-6 parts of silkworm pupa powder. Then, place the auxiliary materials and nutrient solution in a container according to the ratio of 1:1.2-1.

5. Then, sterilize the auxiliary materials and nutrient solution according to conventional methods to obtain culture medium. (7) In a sterile environment, mix the Cordyceps militaris liquid inoculum into the culture medium at a ratio of (3-5):(100-120), and then stir evenly to obtain the culture medium. The above ratio is a weight ratio.

2. The method for cultivating Cordyceps militaris using turtles and tortoises as parasites according to claim 1, characterized in that, The steps for dispensing the culture medium into the shells of turtles or tortoises are as follows: (1) Spray the liquid Cordyceps militaris inoculum evenly over the entire turtle or tortoise shell under sterile conditions; (2) Under sterile conditions, the culture medium containing Cordyceps militaris liquid inoculum can be dispensed into the turtle or tortoise shell through the inoculum outlets set in the turtle or tortoise shell.

3. The method for cultivating Cordyceps militaris using turtles and tortoises as parasites according to claim 1, characterized in that, The cultivation device comprises an incubator, a cultivation rack, LED strip lights, an ozone generator, an ultrasonic humidifier, an exhaust fan, a humidity control instrument, a time relay, a time control switch, and an air humidity regulating instrument. The cultivation rack is placed inside the incubator; the ultrasonic humidifier is placed at the bottom of the incubator; the LED strip lights are placed on the inner walls of the incubator on the front, back, left, and right sides; the oxygen outlet pipe of the ozone generator is connected to the inside of the incubator; and the exhaust fan is installed at the top of the incubator. The humidity control instrument controls the operation of the ultrasonic humidifier; the time relay controls the on / off state of the LED strip lights; the time control switch controls the operation of the ozone generator; and the air humidity regulating instrument controls the operation of the exhaust fan.

4. The method for cultivating Cordyceps militaris using turtles and tortoises as parasites according to claim 1, characterized in that, The cultivation process of the turtle or tortoise pupa cordyceps complex in the aforementioned culture device is as follows: (1) Disinfection of the device: Start the timer switch to run the ozone generator and disinfect the inside of the incubator. (2) Dark light cultivation: Place the turtle or tortoise shell containing the culture medium on the cultivation rack, and then carry out dark light cultivation until the Cordyceps militaris mycelium fills the culture medium inside the turtle or tortoise shell and even the entire turtle or tortoise shell is covered with mycelium. (3) Light cultivation: Activate the time relay and turn on the light strip to expose the entire turtle or tortoise shell to light evenly until all the mycelium on the surface of the turtle or tortoise shell changes color; (4) Scratching: After the mycelium changes color for 2-3 days, scratch the mycelium to induce the germination of fruiting bodies; (5) Water filling and disinfection of the device: Fill the bottom of the incubator with clean water so that the water level submerges the ultrasonic atomizing humidifier. Then turn on the timer switch to run the ozone generator so that the ozone can be disinfected by passing through the ozone ventilation pipe to the clean water filled at the bottom of the incubator. (6) Fruiting body cultivation: When the culture medium on the turtle or tortoise shell begins to germinate fruiting bodies, set the opening and closing time control parameters of the time relay according to the requirements, so that the time relay can effectively control the light strip according to the set opening and closing time. Set the humidity control parameters of the humidity control instrument according to the requirements, so that the humidity control instrument can effectively control the ultrasonic atomizing humidifier according to the set humidity control parameters, so that the humidity environment in the incubator meets the requirements. Set the opening and closing time control parameters of the time control switch according to the requirements, so that the time control switch can effectively control the ozone generator according to the set parameters, and use ozone disinfection to keep the inside of the incubator clean to meet the requirements. Set the humidity control parameters of the air humidity regulating instrument according to the requirements, so that the air humidity regulating instrument can effectively control the exhaust fan according to the set humidity control parameters, so that the humidity environment in the incubator meets the requirements; through intermittent ozone disinfection and reasonable control of light and humidity, the turtle or tortoise is covered with Cordyceps militaris fruiting bodies to obtain a turtle or tortoise Cordyceps militaris complex.

5. The method for cultivating Cordyceps militaris using turtles and tortoises as parasites according to claim 1, characterized in that, The aforementioned turtle or tortoise cordyceps complex is soaked in baijiu (Chinese liquor) to obtain turtle or tortoise cordyceps liquor products. The soaking steps are as follows: remove the mycelium and mycelium from the head, feet, and tail of the turtle or tortoise cordyceps complex and set aside; then place the processed turtle or tortoise cordyceps complex in a liquor bottle, and then pour 30-60 degree baijiu into the bottle to soak for more than 30 days to obtain the finished turtle or tortoise cordyceps liquor products.

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