Artificial insemination and fertilized egg hatching process for silverfish

Abstract

This invention discloses a process for artificial insemination and hatching of fertilized eggs in silverfish, including an artificial insemination process and a fertilized egg hatching process. One step in the artificial insemination process involves operating a swirling device to create a swirling flow between a mixture of male silverfish sperm and a mixture of female silverfish eggs in two containers. This causes the highly viable male silverfish sperm and plump female silverfish eggs to move towards the edge under pressure, until they enter the channel tangentially along the edge for fertilization. In this invention, by using a swirling flow to move the highly viable male silverfish sperm and plump female silverfish eggs towards the edge and simultaneously move them tangentially into the channel to complete fertilization, this method offers the advantage of matching high-quality silverfish sperm with high-quality silverfish eggs, significantly improving the quality of fertilized eggs and the hatching success rate. Furthermore, the morphology of sperm or eggs under swirling flow can be observed using an electron microscope, allowing for selective fertilization.

Description

Technical Field

[0001] This invention relates to the field of fish breeding technology, specifically a process for artificial insemination of silverfish and hatching of fertilized eggs. Background Technology

[0002] Silverfish, also known as noodle fish or stick fish, are several inches long and have a slightly rounded body. Except for the black spots around their eyes, the whole fish is white and transparent, with soft bones and no scales. The peak season for fishing is from mid-May to late June each year.

[0003] Currently, existing artificial insemination and hatching processes for silverfish mostly involve mixing mature silverfish sperm and eggs for artificial insemination. However, a large quantity of mature silverfish sperm and eggs does not necessarily indicate high quality. This can lead to a lower success rate of fertilization between high-quality silverfish sperm and low-quality silverfish eggs, which is detrimental to improving hatching rates. Therefore, to address these issues, a new artificial insemination and fertilized egg hatching process for silverfish is proposed. Summary of the Invention

[0004] The purpose of this invention is to provide a process for artificial insemination of silverfish and hatching of fertilized eggs in order to solve the above-mentioned problems.

[0005] This invention achieves the above objectives through the following technical solution: a process for artificial insemination of silverfish and hatching of fertilized eggs, comprising an artificial insemination process and a fertilized egg hatching process, specifically as follows: The artificial insemination process includes the following steps: A1. According to the ratio of male silverfish semen to female silverfish eggs of 1:3, take out the male silverfish semen and female silverfish eggs and place them in two containers of the same hydrocyclone device, and inject sufficient 4-8‰ physiological saline into each container. A2. The swirling device is used to put the mixture of male silverfish sperm and female silverfish eggs in two of the containers into a swirling state, which causes the highly active male silverfish sperm and the plump female silverfish eggs to move towards the edge under force until they enter the channel along the edge tangentially for fertilization, and finally flow together into the next container. A3. Use an electron microscope to observe the distribution of the remaining male silverfish sperm and female silverfish eggs inside two of the containers to determine whether to adjust the vortex intensity and continue fertilization. A4. Connect the filtration equipment to the next container to perform water filtration and cleaning on the fertilized eggs; A5. After filtering and cleaning, the fertilized eggs are sent to the incubation room for the fertilized egg incubation process.

[0006] Preferably, the fertilized egg incubation process includes the following steps: B1. Early incubation: Pour the fertilized eggs into the incubation container, add enough clean water to submerge the fertilized eggs and artificial vegetation to facilitate the attachment of the fertilized eggs. Control the incubation temperature at 16-20℃, keep the container sheltered from the wind, provide light, and avoid direct sunlight. Regularly observe and test, change the water and disinfect. The incubation container is equipped with a circulating water purifier. B2. Post-harvest management: After the fertilized eggs begin to hatch, stop changing and disinfecting the water, and add an appropriate amount of clean water at a temperature of 2-18℃. After 2 to 4 days of continued rearing, release the fry that have begun to swim horizontally into the breeding pond.

[0007] Preferably, the circulating water purifier operates once every 30 minutes, and the circulating water purifier has a peristaltic-driven fluid flow structure.

[0008] Preferably, the swirling device includes a male silverfish semen container, a female silverfish egg container, a fertilized egg container, a channel, and a staggered plate located in the middle of the channel, all placed on a carrier. The male silverfish semen container, the female silverfish egg container, and the fertilized egg container are interconnected through the same channel. The bottom of the male silverfish semen container and the bottom of the fertilized egg container are both cone-shaped structures, used to form a height gradient of sperm or egg swirling, moving the highly viable male silverfish sperm and the plump female silverfish eggs to the higher edge.

[0009] Preferably, both the male silverfish semen container and the female silverfish egg container are equipped with magnetic stirring rods, and the magnetic stirring rods agitate the liquid in the container to form a vortex through a configured magnetic attraction drive device.

[0010] Preferably, the specific method for observing the morphology of sperm or eggs in the low-speed swirling saline at the edge and middle regions using an electron microscope to determine whether to adjust the swirling intensity to continue fertilization is as follows: if the sperm or eggs at the edge safely pass through the channel for fertilization into the next container, and the morphology of the sperm or eggs in the middle region meets the fertilization standard, then the swirling intensity is adjusted and enhanced under electron microscope observation so that the sperm or eggs in the middle region enter the channel to complete fertilization.

[0011] The beneficial effects of this invention are as follows: By using a swirling flow method to mix male silverfish sperm and female silverfish eggs, the highly viable male silverfish sperm and plump female silverfish eggs are forced to move towards the edge and simultaneously move out and into the channel along the tangential direction to complete fertilization. This method has the advantage of matching high-quality silverfish sperm with high-quality silverfish eggs for fertilization, significantly improving the quality of fertilized eggs and the hatching success rate. By observing the morphology of sperm or eggs under the swirling flow using an electron microscope, selective fertilization can be carried out, allowing for the grading of fertilized eggs and providing a basis for subsequent hatching control conditions. Attached Figure Description

[0012] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0013] Figure 1 This is a schematic diagram of the cyclone device structure of the present invention; Figure 2 This is a demonstration diagram of the operation of the cyclone device of the present invention.

[0014] In the diagram: 1. Cyclone device; 110. Carrier; 111. Male silverfish semen container; 112. Female silverfish egg container; 113. Fertilized egg container; 114. Channel; 115. Interleaved plate; 120. Magnetic stirring rod. Detailed Implementation

[0015] To make the objectives, features, and advantages of this invention more apparent and understandable, the technical solutions of the embodiments of this invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the embodiments described below are only some embodiments of this invention, and not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention.

[0016] The technical solution of the present invention will be further described below with reference to the accompanying drawings and specific embodiments.

[0017] In the description of this invention, it should be understood that the terms "upper", "lower", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0018] Please see Figure 1-2 As shown, a process for artificial insemination and hatching of fertilized eggs in silverfish includes an artificial insemination process and a fertilized egg hatching process, specifically as follows: The artificial insemination process includes the following steps: A1. According to the ratio of male silverfish semen to female silverfish eggs of 1:3, take out the male silverfish semen and female silverfish eggs and place them in two containers of the same hydrocyclone device, and inject sufficient 4-8‰ physiological saline into each container. A2. The swirling device 1 puts the mixture of male silverfish sperm and female silverfish eggs in two of the containers into a swirling state, which causes the highly active male silverfish sperm and the plump female silverfish eggs to move towards the edge under force until they enter the channel along the edge tangentially for fertilization, and finally flow together into the next container. A3. Use an electron microscope to observe the distribution of the remaining male silverfish sperm and female silverfish eggs inside two of the containers to determine whether to adjust the vortex intensity and continue fertilization. A4. Connect the filtration equipment to the next container to perform water filtration and cleaning on the fertilized eggs; A5. After filtering and cleaning, the fertilized eggs are sent to the incubation room for the fertilized egg incubation process.

[0019] The fertilized egg hatching process includes the following steps: B1. Early incubation: Pour the fertilized eggs into the incubation container, add enough clean water to submerge the fertilized eggs and artificial vegetation to facilitate the attachment of the fertilized eggs. Control the incubation temperature at 16-20℃, keep the container sheltered from the wind, provide light, and avoid direct sunlight. Regularly observe and test, change the water and disinfect. The incubation container is equipped with a circulating water purifier. B2. Post-harvest management: After the fertilized eggs begin to hatch, stop changing and disinfecting the water, and add an appropriate amount of clean water at a temperature of 2-18℃. After 2 to 4 days of continued rearing, release the fry that have begun to swim horizontally into the breeding pond.

[0020] The circulating water purifier runs once every 30 minutes, and the circulating water purifier has a peristaltic-driven fluid flow structure.

[0021] The swirling device 1 includes a male silverfish semen container 111, a female silverfish egg container 112, a fertilized egg container 113, a channel 114, and a spaced staggered plate 115 located in the middle of the channel 114, all placed on a carrier 110. The male silverfish semen container 111, the female silverfish egg container 112, and the fertilized egg container 113 are interconnected through the same channel 114. The bottom of the male silverfish semen container 111 and the bottom of the fertilized egg container 113 are both cone-shaped structures, which are used to form a height gradient of sperm or egg swirling, moving the highly active male silverfish sperm and the plump female silverfish eggs to the higher edge.

[0022] Both the male silverfish semen container 111 and the female silverfish egg container 112 are equipped with magnetic stirring rods 120, and the magnetic stirring rods 120 agitate the liquid in the container to form a vortex through a configured magnetic attraction drive device.

[0023] The specific method for observing the morphology of sperm or eggs in the edge and middle regions of low-speed swirling saline using an electron microscope to determine whether to adjust the swirling intensity to continue fertilization is as follows: If the sperm or eggs at the edge safely pass through the channel and enter the next container for fertilization, and the morphology of the sperm or eggs in the middle region meets the fertilization standard, then the swirling intensity is adjusted and increased under electron microscope observation so that the sperm or eggs in the middle region can enter the channel to complete fertilization.

[0024] Compared with existing technologies, the difference lies in the following: by using a swirling flow method to mix male silverfish sperm and female silverfish eggs, the highly viable male silverfish sperm and plump female silverfish eggs are forced to move towards the edge and simultaneously move out and into the channel along the tangential direction to complete fertilization. This method has the advantage of matching high-quality silverfish sperm with high-quality silverfish eggs, significantly improving the quality of fertilized eggs and the hatching success rate. Furthermore, by using an electron microscope to observe the morphology of sperm or eggs under swirling conditions, selective fertilization can be carried out, allowing for the grading of fertilized eggs and providing a basis for subsequent hatching control conditions.

[0025] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of the equivalent elements of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0026] The above-described embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A process for artificial insemination of silverfish and incubation of fertilized eggs, characterized in that: This includes the artificial insemination process and the fertilized egg hatching process, specifically: The artificial insemination process includes the following steps: A1. According to the ratio of male silverfish semen to female silverfish eggs of 1:3, take out the male silverfish semen and female silverfish eggs and place them in two containers of the same hydrocyclone device, and inject sufficient 4-8‰ physiological saline into each container. A2. Running the vortex device (1) The mixture of male silverfish sperm and female silverfish eggs in two of the containers are in a vortex state, which causes the vigorous male silverfish sperm and the plump female silverfish eggs to move towards the edge under force until they enter the channel for fertilization along the edge tangential direction, and finally flow together into the next container. A3. Use an electron microscope to observe the distribution of the remaining male silverfish sperm and female silverfish eggs inside two of the containers to determine whether to adjust the vortex intensity and continue fertilization. A4. Connect the filtration equipment to the next container to perform water filtration and cleaning on the fertilized eggs; A5. After filtering and cleaning, the fertilized eggs are sent to the incubation room for the fertilized egg incubation process. The swirling device (1) includes a male silverfish semen container (111), a female silverfish egg container (112), a fertilized egg container (113), a channel (114), and a staggered plate (115) located in the middle of the channel (114) placed on a carrier (110). The male silverfish semen container (111), the female silverfish egg container (112), and the fertilized egg container (113) are interconnected through the same channel (114). The bottom of the male silverfish semen container (111) and the bottom of the fertilized egg container (113) are both cone-shaped structures, which are used to form a swirling height gradient of sperm or egg, so as to move the vigorous male silverfish sperm and the plump female silverfish eggs to the higher edge.

2. The artificial insemination and fertilized egg hatching process for silverfish according to claim 1, characterized in that: The fertilized egg hatching process includes the following steps: B1. Early incubation: Pour the fertilized eggs into the incubation container, add enough clean water to submerge the fertilized eggs and artificial vegetation to facilitate the attachment of the fertilized eggs. Control the incubation temperature at 16-20℃, keep the container sheltered from the wind, provide light, and avoid direct sunlight. Regularly observe and test, change the water and disinfect. The incubation container is equipped with a circulating water purifier. B2. Post-harvest management: After the fertilized eggs begin to hatch, stop changing and disinfecting the water, and add an appropriate amount of clean water at a temperature of 2-18℃. After 2 to 4 days of continued rearing, release the fry that have begun to swim horizontally into the breeding pond.

3. The artificial insemination and fertilized egg hatching process for silverfish according to claim 2, characterized in that: The circulating water purifier runs once every 30 minutes, and the circulating water purifier has a peristaltic-driven fluid flow structure.

4. The artificial insemination and fertilized egg hatching process for silverfish according to claim 1, characterized in that: Both the male silverfish semen container (111) and the female silverfish egg container (112) are equipped with magnetic stirring rods (120), and the magnetic stirring rods (120) agitate the liquid in the container to form a vortex through a configured magnetic attraction drive device.

5. The artificial insemination and fertilized egg hatching process for silverfish according to claim 1, characterized in that: The specific method for observing the morphology of sperm or eggs in the edge and middle regions of low-speed swirling saline using an electron microscope to determine whether to adjust the swirling intensity to continue fertilization is as follows: If the sperm or eggs at the edge safely pass through the channel and enter the next container for fertilization, and the morphology of the sperm or eggs in the middle region meets the fertilization standard, then the swirling intensity is adjusted and increased under electron microscope observation so that the sperm or eggs in the middle region can enter the channel to complete fertilization.

Images