一种鸡HSPA8多克隆抗体、制备方法及应用
Chicken HSPA8 recombinant protein was prepared using a prokaryotic expression system, and a high-titer, high-specificity polyclonal antibody was successfully prepared, solving the problem of the lack of chicken HSPA8 protein detection antibodies on the market and providing support for research on the function of chicken HSPA8 protein.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- YANGZHOU UNIV
- Filing Date
- 2025-07-28
- Publication Date
- 2026-07-17
AI Technical Summary
The lack of effective antibodies for detecting chicken HSPA8 protein on the market limits in-depth research on the function of chicken HSPA8 protein.
Chicken HSPA8 recombinant protein was prepared using a prokaryotic expression system, and high-titer, high-specificity polyclonal antibodies were prepared by immunizing animals with this protein. The process included recombining the HSPA8 gene into a prokaryotic expression vector, constructing a recombinant plasmid, purifying the host protein, immunizing animals and separating serum, and finally purifying and determining the antibody titer.
A polyclonal antibody that can specifically recognize chicken HSPA8 protein was successfully prepared, which can be used to identify the expression and cellular localization of endogenous protein, filling a market gap and providing a reliable tool for studying the function of chicken HSPA8 protein.
Smart Images
Figure CN120842382B_ABST