Type IIS enzyme-based DNA logic circuit and implementation method thereof

CN120851144APending Publication Date: 2025-10-28SHENYANG AEROSPACE UNIVERSITY
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Patent Information

Application Number
CN202510881614.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-06-27
Publication Date
2025-10-28

AI Technical Summary

Technical Problem

Existing DNA logic gate technologies suffer from challenges such as difficult logic gate structure design, signal attenuation issues, and insufficient response speed and computational accuracy, which limit the practical application of DNA computing.

Method used

By leveraging the specific recognition and efficient cleavage capabilities of Type IIS enzymes, DNA logic gates were constructed to realize YES, AND, and OR gates. Special attention was paid to cascaded AND-AND and cascaded YES-OR gates. Utilizing the specific recognition and cleavage characteristics of Type IIS enzymes, DNA logic circuits were designed to realize logical operations of information through strand substitution reactions.

Benefits of technology

It improves the operation speed and efficiency of DNA logic circuits, enhances the stability and accuracy of the system, simplifies the construction process, promotes the modularity and programmability of biological computing systems, and is suitable for complex biological information processing and decision-making functions.

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Abstract

The invention discloses a DNA logic circuit based on a Type IIS enzyme and an implementation method of the DNA logic circuit, and relates to the field of biological calculation. The Type IIS enzyme participates as a novel restriction endonuclease, so that layered control of a DNA logic gate and a logic circuit can be realized, a non-palindromic DNA sequence can be identified, enzyme digestion is carried out at a specific distance outside a DNA double-strand identification sequence to generate an accurate terminal sequence, the position except an identification site can be any nucleotide combination, and the Type IIS enzyme can be used for identifying the non-palindromic DNA sequence. And more accurate and controllable application can be carried out while the design freedom degree is improved. A DNA substrate is designed to be a four-chain compound, and the sequence of the DNA substrate comprises a recognition site of a specific enzyme. The multi-output DNA strand displacement reaction of the compound is realized through special enzyme digestion, so that the efficiency of DNA logic operation is improved, and the flexibility of a DNA branch migration process and the complexity of molecular self-assembly are improved.
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