一种青稞白粉病抗性相关的KASP分子标记方法及其应用
By developing a KASP molecular marker method for barley powdery mildew resistance, and utilizing specific primers and a real-time PCR instrument, the problem of screening and identifying barley powdery mildew resistant resources in existing technologies has been solved. This has enabled efficient, accurate, and rapid breeding-assisted identification, thereby improving breeding efficiency.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- AGRI RES INST TIBET ACADEMY OF AGRI & ANIMAL HUSBANDRY SCI
- Filing Date
- 2025-08-26
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies make it difficult to efficiently and accurately screen and identify germplasm resources related to powdery mildew resistance in highland barley, affecting breeding efficiency and breeding level.
A KASP molecular marker method for resistance to powdery mildew in barley was developed. Using a combination of molecular marker primers consisting of specific upstream and downstream primers, gene loci were identified by fluorescent probes. Primer sequences targeting key SNP sites on the second chromosome of barley were designed and genotyping was performed using a real-time PCR instrument.
It enables efficient, accurate, rapid, and low-cost identification of powdery mildew resistance in highland barley, simplifies the operation process, and improves breeding efficiency and breeding level.
Smart Images
Figure CN120866566B_ABST