An effective repellent for protecting pine trees from pine wilt disease and its application method.

Abstract

This application relates to the technical field of pest control, and in particular to an effective repellent for protecting pine trees from pine wilt disease and its application method. The method for applying the repellent includes the following steps: dividing the forest into circular areas to create multiple circular zones; placing the repellent at the center point of each circular zone; and within each circular zone, setting up a pine sawyer beetle trap in a direction spreading outwards from the center of the repellent towards the circumference of the zone, luring the pine sawyer beetles into the traps; the distance between the repellent and the pine sawyer beetle trap is 0-40 meters. This application discloses an effective repellent for protecting pine trees from pine wilt disease and its application method. By optimizing the hanging distance between the repellent and the pine sawyer beetle trap, and the interval for replacing the repellent, the method effectively protects pine trees from pine wilt disease.

Description

Technical Field

[0001] This application relates to the technical field of pest control, and in particular to a repellent and its application method for effectively protecting pine trees from pine wilt disease. Background Technology

[0002] Pine wilt disease is a devastating disease of pine trees, commonly known as pine wilt. It is highly virulent, has a long incubation period, and infected pine trees can wither and die within months, earning it the reputation of being the "cancer" of pine trees. Currently, Asia is the region most severely affected by pine wilt disease.

[0003] Judging from the development trend in recent years, the pine wilt disease epidemic is not optimistic and is showing an intensifying trend. Many famous scenic spots, world natural and cultural heritage sites, ancient pines, and key ecological areas are facing disaster crises, seriously threatening the development of pine forest areas and related forestry economies. It is the most dangerous number one forest disease.

[0004] Pine wilt disease is primarily transmitted by the pine sawyer beetle. Currently, prevention and protection of famous and ancient pines from pine wilt nematode infection mainly rely on trunk injection of pesticides, pesticides to control the pine sawyer beetle, and physical trapping of the beetle. However, because famous and ancient pines are important heritage sites with immense cultural value, the current control methods may increase the risk of further infection, directly or indirectly causing secondary damage to these trees. Furthermore, publicly available methods for protecting pine trees using repellents do not consider the practical effectiveness and cost of application.

[0005] Therefore, optimizing the distance between the repellent and the pine wilt disease trap, as well as the interval for replacing the repellent, to effectively protect famous and ancient pines from pine wilt disease has become an urgent problem to be solved. Summary of the Invention

[0006] To address the shortcomings of existing technologies, this application provides an effective repellent for protecting pine trees from pine wilt disease and a method for its application. When using the repellent, simply hang it on the pine tree to be protected or on surrounding trees and buildings to prevent the pine sawyer beetle from feeding on the protected pine tree and thus spreading pine wilt disease.

[0007] Firstly, this application provides a method for applying a repellent to effectively protect pine trees from pine wilt disease, employing the following technical solution: A method for applying a repellent to effectively protect pine trees from pine wilt disease includes the following steps: The forest is divided into circular areas, thus establishing multiple circular areas; Place a repellent agent at the center point of each circular area; Within each circular area, starting from the repellent placed at the center point, traps for the pine beetle are set up in a direction that spreads outward from the center to the circumference of the circular area, and the pine beetles are lured into the traps.

[0008] Preferably, the pine beetle trap contains a pine beetle attractant.

[0009] By adopting the above technical solution, this application uses multiple circular areas to drive the pine sawyer beetle into the pine sawyer beetle trap, which can more thoroughly trap the pine sawyer beetle in the area. While saving on the cost of pesticides, economic costs and labor costs, it can make the repellent effect of the repellent the best, thereby effectively protecting pine trees from pine wilt disease.

[0010] Preferably, the distance between the repellent and the pine beetle trap is 0-40 meters.

[0011] More preferably, the distance between the repellent and the pine beetle trap is 0-20 meters.

[0012] By adopting the above technical solution and limiting the distance between the repellent and the longhorn beetle trap, the repellent effect can be optimized while saving on drug and labor costs.

[0013] Preferably, the repellent is replaced in the following steps: the repellent is replaced every 15-45 days.

[0014] A further preferred option is an interval of 15-30 days.

[0015] By adopting the above technical solution and limiting the interval between repellent replacements, the repellent's repellent effect can be optimized while saving on drug and labor costs.

[0016] This application limits the distance between the repellent and the longhorn beetle trap, as well as the interval between repellent replacements, thus avoiding the subjective notion that a smaller spacing between repellent placements and a more frequent replacement frequency would achieve a protective effect. This reduces the economic cost of application and the waste of reagents.

[0017] Preferably, the repellent comprises the following raw material components in parts by weight: 10-30 parts of 4-allyl anisole, 10-30 parts of beta-benzyl acrolein, 60-80 parts of ethanol, 2-8 parts of Tween-80, and 10-20 parts of linalool.

[0018] Preferably, the repellent further includes 10-16 parts by weight of eugenol.

[0019] Preferably, the repellent further includes 5-15 parts by weight of benzoyl acetate and 5-15 parts by weight of vanillin.

[0020] Preferably, the repellent further comprises 3.5-4.5 parts by weight of stearic acid and 2.5-3.5 parts by weight of isopropyl palmitate.

[0021] By adopting the above technical solution, this application can significantly improve the repellency effect of the repellent through the improvement of raw materials and the synergistic effect between the raw materials.

[0022] Preferably, the repellent further includes 30-50 parts by weight of a coating agent; the coating agent comprises the following raw material components in parts by weight: 30-50 parts gelatin, 5-15 parts quaternized chitosan, 7-13 parts glycerol, 1.6-2.5 parts linalool, 0.45-0.6 parts Tween-80, and 10-15 parts rice starch.

[0023] By adopting the above technical solution, the added film-forming agent encapsulates the drug and forms a dense protective film on the drug surface, which can effectively reduce drug dosage fluctuations, enhance drug efficacy, improve drug utilization efficiency, and reduce the potential impact of the drug on the environment. The added film-forming agent can slow down the release of the drug, prolong the action time of the active ingredient, reduce the number of applications, and reduce the total amount of drug applied. It also mitigates or even avoids the degradation of the active ingredient caused by environmental and other factors.

[0024] In this application, by improving the raw materials, the addition of quaternized chitosan and linalool creates a complex mixture. The positive charge of the quaternized chitosan combines with the volatile components of linalool to form a dual effect of positive charge interference and odor interference, which can effectively improve the repellency of the repellent. At the same time, the synergistic effect between the added quaternized chitosan, gelatin, rice starch, and linalool can form a denser protective layer.

[0025] Preferably, the preparation method of the coating agent includes the following steps: Gelatin and quaternized chitosan were mixed, glycerol was added and mixed, then the first portion of linalool was added and mixed, and finally Tween-80 was added and mixed to obtain a complex. Mix rice starch with water, heat at 90-100℃ for 1-2 hours, cool down to 20-30℃, add the second part of linalool and mix, then heat to 30-50℃, add the complex and mix to obtain the coating agent. The mass ratio of the first linalool to the second linalool is (1-2):(0.1-1.5).

[0026] By adopting the above technical solution, this application uses linalool, quaternized chitosan and gelatin as raw materials, and uses the surfactant Tween-80 to coat linalool to form an emulsion; then adds a mixture obtained by blending rice starch and linalool to prepare a coating agent with good stability. The prepared coating agent forms a dense protective layer on the surface of the drug, effectively extending the service life of the drug. At the same time, the coating agent has good degradability and is environmentally friendly.

[0027] This application discloses an effective repellent for protecting pine trees from pine wilt disease and its application method, which has the following advantages: First, it does not use chemical pesticides; the repellent itself does not kill longhorn beetles, and its special odor can cause longhorn beetles to evade or repel them, without harming the protected pine trees or other non-target organisms. Second, it avoids the risk of pine wilt disease transmission caused by the aggregation of longhorn beetles when using pine sawyer beetle traps alone. Third, it avoids the risk of infection when using chemical control of pine sawyer beetles in existing technologies, where not all longhorn beetles are killed and surviving longhorn beetles still pose a risk of pine wilt disease infection. Fourth, it optimizes the hanging distance between the repellent and the pine sawyer beetle trap, as well as the interval for replacing the repellent, avoiding the subjective idea that a smaller hanging distance and a faster replacement frequency of the repellent will achieve the protective effect, thus reducing the economic cost of application and the waste of pesticides.

[0028] In summary, this application includes at least one of the following beneficial technical effects: This application discloses an effective repellent for protecting pine trees from pine wilt disease and its deployment method. By limiting the hanging distance between the repellent and the pine sawyer beetle trap and the interval for replacing the repellent, the repellent can prevent and protect pine trees from pine wilt disease, while effectively improving the repellent's repellency and saving economic costs. In this application, by improving the raw materials of the repellent, a coating agent is added to form a dense protective layer on the surface of the agent, which effectively improves the repellency effect of the repellent and extends the service life of the agent. Detailed Implementation

[0029] The technical solutions of this application are further illustrated by specific embodiments below. These specific embodiments do not represent a limitation on the scope of protection of this application. Any non-essential modifications and adjustments made by others based on the concept of this application still fall within the scope of protection of this application.

[0030] All raw materials involved in this application are commercially available products, including: The indica rice was purchased from Hefei Jinyuchao Food Co., Ltd. Gelatin, purchased from Hebei Huafeng Adhesives Co., Ltd.; Quaternized chitosan, with a degree of substitution of 90%, was purchased from Qingdao Haipu Biotechnology Co., Ltd. Glycerin, purchased from Jiangsu Xinhua Glycerin Technology Co., Ltd.; 4-Allyl anisole, also known as Artemisia capillaris; beta-benzenecrolein, also known as cinnamon aldehyde; Pine wood nematode trap, photoelectric intelligent trap for pine wood nematode vector longhorn beetle, model SYYZ-8035A, SYYZ-8035B, purchased from Nanjing Shengxing Pest Control Technology Co., Ltd. Pine wood nematode attractant, model SY3, purchased from Nanjing Shengxing Pest Control Technology Co., Ltd. The present application will be further described in detail below with reference to embodiments and comparative examples.

[0031] Raw material source: The preparation method of indica rice starch is as follows: (1) Grind the rice into powder with a particle size of no more than 80 mesh and defatt it with petroleum ether at a ratio of 1:2 (g / mL). (2) Disperse the defatted sample in 0.2% NaOH solution (1:4, g / mL) and soak it. Discard the supernatant. Then wash the rice flour several times until white starch is obtained from the precipitate. (3) Dry in an oven at 40°C and grind to obtain rice starch through a 100-mesh sieve.

[0032] Preparation Example 1: Preparation of the coating agent: The coating agent comprises the following raw material components: 40 kg of gelatin, 10 kg of quaternized chitosan, 10 kg of glycerin, 2 kg of linalool, 0.5 kg of Tween-80, and 13 kg of rice starch.

[0033] The preparation method of the coating agent is as follows: After mixing gelatin and quaternized chitosan, glycerol was added and mixed at 60°C for 1 hour. Then, the first portion of linalool was added and mixed for 10 minutes. Finally, Tween-80 was added and mixed for 20 minutes to obtain the complex. Add 500 mL of water to rice starch and mix. Heat at 95°C for 1.5 hours, then cool to 25°C. Add the second part of linalool and mix at 40°C for 30 minutes. Then add the complex and mix for another 30 minutes to obtain the coating agent.

[0034] The mass ratio of the first linalool to the second linalool is 1.5:0.5.

[0035] Preparation Example 2: Preparation of the coating agent: The coating agent comprises the following raw material components: 30 kg of gelatin, 5 kg of quaternized chitosan, 7 kg of glycerin, 1.6 kg of linalool, 0.45 kg of Tween-80, and 10 kg of rice starch.

[0036] The preparation method of the coating agent is as follows: After mixing gelatin and quaternized chitosan, glycerol was added and mixed at 60°C for 1 hour. Then, the first portion of linalool was added and mixed for 10 minutes. Finally, Tween-80 was added and mixed for 20 minutes to obtain the complex. Add 500 mL of water to rice starch and mix. Heat at 95°C for 1.5 hours, then cool to 25°C. Add the second part of linalool and mix at 40°C for 30 minutes. Then add the complex and mix for another 30 minutes to obtain the coating agent.

[0037] The mass ratio of the first linalool to the second linalool is 1:0.6.

[0038] Preparation Example 3: Preparation of the coating agent: The coating agent comprises the following raw material components: 50 kg of gelatin, 15 kg of quaternized chitosan, 13 kg of glycerol, 2.5 kg of linalool, 0.6 kg of Tween-80, and 15 kg of rice starch.

[0039] The preparation method of the coating agent is as follows: After mixing gelatin and quaternized chitosan, glycerol was added and mixed at 60°C for 1 hour. Then, the first portion of linalool was added and mixed for 10 minutes. Finally, Tween-80 was added and mixed for 20 minutes to obtain the complex. Add 500 mL of water to rice starch and mix. Heat at 95°C for 1.5 hours, then cool to 25°C. Add the second part of linalool and mix at 40°C for 30 minutes. Then add the complex and mix for another 30 minutes to obtain the coating agent.

[0040] The mass ratio of the first linalool to the second linalool is 2:0.5.

[0041] Example 1: The preparation method of the liquid repellent is as follows: 20 kg of 4-allyl anisole, 20 kg of beta-benzenepropenal, 70 kg of anhydrous ethanol, 5 kg of Tween-80, and 15 kg of linalool were stirred and mixed to obtain a liquid repellent.

[0042] Example 2: The preparation method of the liquid repellent is as follows: 10 kg of 4-allyl anisole, 10 kg of beta-benzyl acrolein, 60 kg of anhydrous ethanol, 2 kg of Tween-80, and 10 kg of linalool were stirred and mixed to obtain a liquid repellent.

[0043] Example 3: The preparation method of the liquid repellent is as follows: Mix 30 kg of 4-allyl anisole, 30 kg of beta-benzenepropenal, 80 kg of anhydrous ethanol, 8 kg of Tween-80, and 20 kg of linalool to obtain a liquid repellent.

[0044] Example 4: The difference from Example 1 is that the liquid repellent also includes 13 kg of eugenol.

[0045] Example 5: The difference from Example 2 is that the liquid repellent also includes 10 kg of eugenol.

[0046] Example 6: The difference from Example 3 is that the liquid repellent also includes 16 kg of eugenol.

[0047] Example 7: The difference from Example 1 is that the liquid repellent also includes 10 kg of benzoyl acetate and 10 kg of vanillin.

[0048] Example 8: The difference from Example 2 is that the liquid repellent also includes 5 kg of benzoyl acetate and 5 kg of vanillin.

[0049] Example 9: The difference from Example 3 is that the liquid repellent also includes 15 kg of benzoyl acetate and 15 kg of vanillin.

[0050] Example 10: The difference from Example 4 is that the liquid repellent also includes 40 kg of coating agent.

[0051] The coating agent was prepared in Preparation Example 1.

[0052] Example 11: The difference from Example 5 is that the liquid repellent also includes 30 kg of coating agent.

[0053] The coating agent was prepared in Preparation Example 2.

[0054] Example 12: The difference from Example 6 is that the liquid repellent also includes 50 kg of coating agent.

[0055] The coating agent was prepared in Preparation Example 3.

[0056] Example 13: The difference from Example 10 is that the liquid repellent also includes 30 kg of coating agent.

[0057] Example 14: The difference from Example 10 is that the liquid repellent also includes 50 kg of coating agent.

[0058] Example 15: The difference from Example 7 is that the liquid repellent also includes 40 kg of coating agent.

[0059] The coating agent was prepared in Preparation Example 1.

[0060] Example 16: The difference from Example 8 is that the liquid repellent also includes 30 kg of coating agent.

[0061] The coating agent was prepared in Preparation Example 2.

[0062] Example 17: The difference from Example 9 is that the liquid repellent also includes 50 kg of coating agent.

[0063] The coating agent was prepared in Preparation Example 3.

[0064] Example 18: The difference from Example 15 is that the liquid repellent also includes 30 kg of coating agent.

[0065] Example 19: The difference from Example 15 is that the liquid repellent also includes 50 kg of coating agent.

[0066] Example 20: The preparation method of solid repellents includes the following steps: Mix 20 kg of 4-allyl anisole, 20 kg of beta-benzyl acrolein, 70 kg of anhydrous ethanol, 5 kg of Tween-80, 15 kg of linalool, and 13 kg of eugenol thoroughly. After adding 4 kg of stearic acid and 3 kg of isopropyl palmitate and mixing, the mixture is heated to 60°C. Under stirring conditions, 10 mL of alkaline solution (10% sodium hydroxide solution) is added and stirred continuously to obtain a solid repellent.

[0067] Example 21: The preparation method of solid repellents includes the following steps: Mix 10 kg of 4-allyl anisole, 10 kg of beta-benzyl acrolein, 60 kg of anhydrous ethanol, 2 kg of Tween-80, 10 kg of linalool, and 10 kg of eugenol thoroughly. After adding 3.5 kg of stearic acid and 2.5 kg of isopropyl hexadecanoate and mixing, the mixture was heated to 60°C. Under stirring conditions, 10 mL of alkaline solution (10% sodium hydroxide solution) was added and stirred continuously to obtain a solid repellent.

[0068] Example 22: The preparation method of solid repellents includes the following steps: Mix 30 kg of 4-allyl anisole, 30 kg of beta-benzyl acrolein, 80 kg of anhydrous ethanol, 8 kg of Tween-80, 20 kg of linalool, and 16 kg of eugenol thoroughly. After adding 4.5 kg of stearic acid and 3.5 kg of isopropyl palmitate and mixing, the mixture was heated to 60°C. Under stirring conditions, 10 mL of alkaline solution (10% sodium hydroxide solution) was added and stirred continuously to obtain a solid repellent.

[0069] Example 23: The difference from Example 20 is that the solid repellent also includes 40 kg of coating agent.

[0070] The coating agent was prepared in Preparation Example 1.

[0071] Example 24: The difference from Example 21 is that the solid repellent also includes 30 kg of coating agent.

[0072] The coating agent was prepared in Preparation Example 2.

[0073] Example 25: The difference from Example 22 is that the solid repellent also includes 50 kg of coating agent.

[0074] The coating agent was prepared in Preparation Example 3.

[0075] Example 26: The difference from Example 23 is that the solid repellent also includes 30 kg of coating agent.

[0076] Example 27: The difference from Example 23 is that the solid repellent also includes 50 kg of coating agent.

[0077] Example 28: The preparation method of solid repellents includes the following steps: Mix 20 kg of 4-allyl anisole, 20 kg of beta-benzyl acrolein, 70 kg of anhydrous ethanol, 5 kg of Tween-80, 15 kg of linalool, 10 kg of benzyl acetate, and 10 kg of vanillin. After adding 4 kg of stearic acid and 3 kg of isopropyl palmitate and mixing, the mixture was heated to 60°C. Under stirring conditions, 10 mL of alkaline solution (10% sodium hydroxide solution) was added and stirred continuously to obtain a solid repellent.

[0078] Example 29: The preparation method of solid repellents includes the following steps: Mix 10 kg of 4-allyl anisole, 10 kg of beta-benzyl acrolein, 60 kg of anhydrous ethanol, 2 kg of Tween-80, 10 kg of linalool, 5 kg of benzyl acetate, and 5 kg of vanillin. After adding 3.5 kg of stearic acid and 2.5 kg of isopropyl hexadecanoate and mixing, the mixture was heated to 60°C. Under stirring conditions, 10 mL of alkaline solution (10% sodium hydroxide solution) was added and stirred continuously to obtain a solid repellent.

[0079] Example 30: The preparation method of solid repellents includes the following steps: Mix 30 kg of 4-allyl anisole, 30 kg of beta-benzyl acrolein, 80 kg of anhydrous ethanol, 8 kg of Tween-80, 20 kg of linalool, 15 kg of benzyl acetate, and 15 kg of vanillin. After adding 4.5 kg of stearic acid and 3.5 kg of isopropyl palmitate and mixing, the mixture was heated to 60°C. Under stirring conditions, 10 mL of alkaline solution (10% sodium hydroxide solution) was added and stirred continuously to obtain a solid repellent.

[0080] Example 31: The difference from Example 28 is that the solid repellent also includes 40 kg of coating agent.

[0081] The coating agent was prepared in Preparation Example 1.

[0082] Example 32: The difference from Example 29 is that the solid repellent also includes 30 kg of coating agent.

[0083] The coating agent was prepared in Preparation Example 2.

[0084] Example 33: The difference from Example 30 is that the solid repellent also includes 50 kg of coating agent.

[0085] The coating agent was prepared in Preparation Example 3.

[0086] Example 34: The difference from Example 31 is that the solid repellent also includes 30 kg of coating agent.

[0087] Example 35: The difference from Example 31 is that the solid repellent also includes 50 kg of coating agent.

[0088] Comparative Example 1: The difference from Example 10 is that the liquid repellent also includes 29 kg of coating agent.

[0089] Comparative Example 2: The difference from Example 10 is that the liquid repellent also includes 51 kg of coating agent.

[0090] Comparative Example 3: The difference from Example 15 is that the liquid repellent also includes 29 kg of coating agent.

[0091] Comparative Example 4: The difference from Example 15 is that the liquid repellent also includes 51 kg of coating agent.

[0092] Comparative Example 5: The difference from Example 23 is that the solid repellent also includes 29 kg of coating agent.

[0093] Comparative Example 6: The difference from Example 23 is that the solid repellent also includes 51 kg of coating agent.

[0094] Comparative Example 7: The difference from Example 31 is that the solid repellent also includes 29 kg of coating agent.

[0095] Comparative Example 8: The difference from Example 31 is that the solid repellent also includes 51 kg of coating agent.

[0096] Performance testing: 1. Laboratory avoidance test 1.1 Preparation of test insects Healthy adult pine sawyer beetles (male to female ratio 1:1) were collected and reared for 3-5 days under laboratory conditions of 25±1℃, relative humidity 70%±5%, and photoperiod 14L:10D. Individuals with consistent activity and similar body size were selected as test insect sources.

[0097] 1.2 Preparation of repellent Liquid repellent: The repellents prepared in Examples 1-19 and Comparative Examples 1-4 were dissolved in ethanol to prepare a repellent with a concentration of 0.6%.

[0098] Solid repellent: Weigh 20g of the solid repellent prepared in Examples 20-35 and Comparative Examples 5-8.

[0099] 1.3 Experimental Setup A three-chamber avoidance box (3m×3m×3m) was used; chamber A of the avoidance box was the treatment area, and chamber C of the avoidance box was the control area. Maintain an airflow velocity of 0.3-0.5 m / s inside the chamber, a temperature of 25 ± 2℃, and a humidity of 70% ± 5%. 1.4. Experimental Material Processing Cut fresh pine branches into 14cm long sections and hang them in repellent boxes A and C respectively. Hang the repellent (liquid or solid) in the treatment area, 10cm away from the test branch; In the control area, blank carriers were hung 10cm away from the test branches; 1.5 Experimental Procedure: Place one male and one female adult pine brown longhorn beetle into repellency box B and observe for 7 days. Each day, branches are taken out, and feeding patches are rubbed onto sulfuric acid paper and the feeding area is calculated. At the same time, the health of the longhorn beetles must be observed, and any deaths must be promptly addressed by replenishing the beetles. While the longhorn beetles are being selectively fed, non-selective feeding experiments are also being conducted.

[0100] Each experiment was set up with 10 replicates.

[0101] 1.6 Calculation of Evasion Effect The calculation method for the repellency effect of repellents is as follows: The repellency effect of repellents is calculated based on the size of the feeding area during the rearing process. The calculation model is as follows: Model: Avoidance Rate (RI) = (CK - T) / CK × 100; Note: In the calculation model, CK represents the feeding area of ​​the longhorn beetle on branches in the control area; T represents the feeding area of ​​the longhorn beetle on branches in the treatment area.

[0102] Table 1. Results of Avoidance Rate Detection Based on the test results of Examples 1-3 and Examples 4-6, it can be seen that the repellency effect of Examples 4-6 is better than that of Examples 1-3. This indicates that the composition of the repellent was improved, and the synergistic effect between the added eugenol and the raw materials further improved the repellency effect of the repellent on the pine sawyer beetle.

[0103] Based on the test results of Examples 1-3 and Examples 7-9, it can be seen that the repellency effect of Examples 7-9 is better than that of Examples 1-3. This indicates that the composition of the repellent was improved, and the synergistic effect between the added benzyl acetate, vanillin and other raw materials further improved the repellency effect of the repellent on the pine beetle.

[0104] The test results of Examples 4-6 and Examples 10-12 show that the repellency effect of Examples 10-12 is better than that of Examples 4-6, indicating that the composition of the repellent was improved and the added coating agent effectively improved the repellency effect of the repellent on the pine sawyer beetle.

[0105] Based on the test results of Examples 10, 13, 14, Comparative Example 1, and Comparative Example 2, it can be seen that the amount of coating agent added has a certain impact on the repellency effect of the repellent, and the repellent effect against the pine beetle is best when the amount of coating agent added is 30-50 kg.

[0106] Based on the test results of Examples 7-9 and Examples 15-17, it can be seen that the repellency rate of Examples 15-17 is better than that of Examples 7-9, indicating that the composition of the repellent was improved and the added coating agent effectively improved the repellency effect of the repellent on the pine longhorn beetle.

[0107] Based on the test results of Examples 15, 18, 19, Comparative Example 3, and Comparative Example 4, it can be seen that the amount of coating agent added has a certain impact on the repellency effect of the repellent, and the repellent effect against the pine sawyer beetle is best when the amount of coating agent added is 30-50 kg.

[0108] Based on the test results of Examples 20-22 and Examples 23-25, it can be seen that the repellency effect of Examples 23-25 ​​is better than that of Examples 20-22, indicating that the composition of the repellent was improved and the added coating agent effectively improved the repellency effect of the repellent on the pine longhorn beetle.

[0109] Based on the test results of Examples 23, 26, 27, Comparative Example 5, and Comparative Example 6, it can be seen that the amount of coating agent added has a certain impact on the repellency effect of the repellent, and the repellent effect against the pine sawyer beetle is best when the amount of coating agent added is 30-50 kg.

[0110] Based on the test results of Examples 28-30 and Examples 31-33, it can be seen that the repellency effect of Examples 31-33 is better than that of Examples 28-30, indicating that the composition of the repellent was improved and the added coating agent effectively improved the repellency effect of the repellent on the pine longhorn beetle.

[0111] Based on the test results of Examples 31, 34, 35, Comparative Example 7, and Comparative Example 8, it can be seen that the amount of coating agent added has a certain impact on the repellency effect of the repellent, and the repellent effect on the pine beetle is best when the amount of coating agent added is 30-50 kg.

[0112] 2. Forest avoidance experiment 2.1 Selection of Experimental Area: Select small plots of pine wilt disease outbreak, divide the sample plots into circular areas, and thus set up multiple circular areas; 2.2 Treatment with repellents: Liquid repellent: The repellents prepared in Examples 1-19 and Comparative Examples 1-4 were dissolved in ethanol to prepare 10L of repellent with a concentration of 0.6%.

[0113] Solid repellent: Weigh 20 kg of the repellent prepared in Examples 20-35 and Comparative Examples 5-8.

[0114] 2.3 Experimental Procedure: During the peak emergence period of the pine sawyer beetle, repellent and traps were suspended. The specific steps are as follows: Hang a repellent (liquid or solid) at the center point of each circular area; Within each circular area, starting from the repellent placed at the center point, pine beetle traps are set out in a direction that spreads outward from the center to the circumference of the circular area, luring the pine beetles into the traps; each pine beetle trap contains a matching pine beetle attractant; the distance between the repellent and the pine beetle trap is 20 meters.

[0115] The control group was equipped only with pine beetle traps.

[0116] The area where the bait was placed was unaffected by the repellent. The number of pine beetles in the traps was counted to evaluate the repellent's effect on the beetles, i.e., its protective effect.

[0117] Calculation method for repellency effect (protective effect): The field application effect of the repellent can be evaluated by measuring the number of pine beetles trapped by the attractant near the repellent. The calculation model is as follows: Where: CK is the number of adult insects captured by the trap in the blank control group; Tr represents the number of adult insects captured by the traps in the treatment group; 2.4 The repellency effect was tested 15 days and 30 days after the application of the repellent. The results of the repellency test are shown in Table 2.

[0118] Table 2. Avoidance Rate Test Results As shown in the table above, the test results of Examples 4-6 and Examples 10-12 indicate that the repellent prepared in Examples 4-6 showed a significant decrease in repellency after 30 days, while the repellent prepared in Examples 10-12 showed no change in repellency after 30 days. This suggests that the added coating agent formed a dense protective layer on the outside of the agent, thereby effectively prolonging the repellent effect on the pine sawyer beetle.

[0119] Based on the test results of Examples 10, 13, 14, Comparative Example 1, and Comparative Example 2, it can be seen that the amount of coating agent added has a certain impact on the effect of the repellent, and the repellent effect on the pine beetle is best when the amount of coating agent added is 30-50 kg.

[0120] Based on the test results of Examples 7-9 and Examples 15-17, it can be seen that the repellent prepared in Examples 7-9 showed a significant decrease in repellency after 30 days, while the repellent prepared in Examples 15-17 showed no change in repellency after 30 days. This indicates that the added coating agent formed a dense protective layer on the outside of the agent, thereby effectively prolonging the repellent effect of the repellent on the pine sawyer beetle.

[0121] Based on the test results of Examples 15, 18, 19, Comparative Example 3, and Comparative Example 4, it can be seen that the amount of coating agent added has a certain impact on the effect of the repellent, and the repellent effect on the pine beetle is best when the amount of coating agent added is 30-50 kg.

[0122] Based on the test results of Examples 20-22 and Examples 23-25, it can be seen that the repellent prepared in Examples 20-22 showed a significant decrease in repellency after 30 days, while the repellent prepared in Examples 23-25 ​​showed no change in repellency after 30 days. This indicates that the added coating agent formed a dense protective layer on the outside of the agent, thereby effectively prolonging the repellent effect of the repellent on the pine sawyer beetle.

[0123] Based on the test results of Examples 23, 26, 27, Comparative Example 5, and Comparative Example 6, it can be seen that the amount of coating agent added has a certain impact on the effect of the repellent, and the repellent effect on the pine sawyer beetle is best when the amount of coating agent added is 30-50 kg.

[0124] Based on the test results of Examples 28-30 and Examples 31-33, it can be seen that the repellent prepared in Examples 28-30 showed a significant decrease in repellency after 30 days, while the repellent prepared in Examples 31-33 showed no change in repellency after 30 days. This indicates that the added coating agent formed a dense protective layer on the outside of the agent, thereby effectively prolonging the repellent effect of the repellent on the pine sawyer beetle.

[0125] Based on the test results of Examples 31, 34, 35, Comparative Example 7, and Comparative Example 8, it can be seen that the amount of coating agent added has a certain impact on the effect of the repellent, and the repellent effect on the pine sawyer beetle is best when the amount of coating agent added is 30-50 kg.

[0126] Example 36: To demonstrate the effect of the distance between the repellent and the pine beetle trap on the repellency rate, the distance between the repellent and the trap was adjusted. With other reaction conditions remaining constant, the effect of this distance on the repellency rate was tested using the following method: 3.1 Selection of Experimental Area Select small plots of pine wilt disease outbreak, divide the sample plots into circular areas, and thus set up multiple circular areas; 3.2 Treatment with repellents: The solid repellent prepared in Example 31 was used as the repellent in this example, and the mass of the repellent was 20 kg.

[0127] The pine beetle trap contains a matching pine beetle attractant.

[0128] 3.3 Experimental Procedure: During the peak emergence period of the pine sawyer beetle, hang repellent and pine sawyer beetle traps at the intervals shown in Table 3. The specific steps are as follows: A repellent agent is suspended at the center point of each circular area; Within each circular area, starting from the repellent placed at the center point, traps for the pine beetle are set up in a direction that spreads outward from the center to the circumference of the circular area, and the pine beetles are lured into the traps.

[0129] The control group was equipped only with pine beetle traps.

[0130] The area where the bait was placed was unaffected by the repellent. The number of pine beetles in the traps was counted to evaluate the repellent's effect on the beetles, i.e., its protective effect.

[0131] Calculation method for repellency effect (protective effect): The field application effect of the repellent can be evaluated by measuring the number of pine beetles trapped by the attractant near the repellent. The calculation model is as follows: Where: CK is the number of adult insects captured by the trap in the blank control group; Tr represents the number of adult insects captured by the traps in the treatment group; The repellency rate of the repellent was calculated by recording data in the forest for 30 days. The results are shown in Table 3.

[0132] Table 3. Effect of the distance between the repellent and the pine beetle trap on the repellency rate. 36.1 0-10 100 36.2 10-20 100 36.3 20-30 79.55 36.4 30-40 72.73 36.5 40-50 47.06 36.6 50-60 18.5 As shown in Table 3, the repellency rate of the repellent exhibits a regular variation at different distances. In particular, the repellency rate against the pine sawyer beetle reaches 100% within the 0-10m and 10-20m installation distances, meaning there is no adult pine sawyer beetle activity within this range, effectively protecting healthy pine trees in that area. Considering both the cost of the repellent and labor costs, this embodiment selects 10-20m as the optimal installation distance for the repellent.

[0133] Example 37: When repellents are suspended on pine trees in the forest, the dosage gradually decreases over time due to continuous volatilization. Below a certain dosage, they lose their repellent effect on pine sawyer beetles. To demonstrate the impact of the application time of the repellent on its repellency effect, the application time was adjusted. Under otherwise constant reaction conditions, the effect of the application time on the repellency rate was tested. The specific steps are as follows: 4.1 Selection of Experimental Area Select small plots of pine wilt disease outbreak, divide the sample plots into circular areas, and thus set up multiple circular areas; 4.2 Treatment with repellents: The solid repellent prepared in Example 31 was used as the repellent in this example, and the mass of the repellent was 20 kg.

[0134] The pine beetle trap contains a matching pine beetle attractant.

[0135] 4.3 Experimental Procedure: During the peak emergence period of the pine sawyer beetle, repellents and beetle traps were suspended, as detailed below: A repellent agent is suspended at the center point of each circular area; Within each circular area, starting from the repellent placed at the center point, traps for the pine beetle are set up in a direction that spreads outward from the center to the circumference of the circular area, and the pine beetles are lured into the traps; the distance between the repellent and the pine beetle traps is 20 meters.

[0136] The control group was equipped only with pine beetle traps.

[0137] The area where the bait was placed was unaffected by the repellent. The number of pine beetles in the traps was counted to evaluate the repellent's effect on the beetles, i.e., its protective effect.

[0138] Calculation method for repellency effect (protective effect): The field application effect of the repellent can be evaluated by measuring the number of pine beetles trapped by the attractant near the repellent. The calculation model is as follows: Where: CK is the number of adult insects captured by the trap in the blank control group; Tr represents the number of adult insects captured by the traps in the treatment group; The results of the repellency test for the repellent agent used in the forest are shown in Table 4.

[0139] Table 4. The effect of repellent application time on repellency rate As shown in Table 4, the repellency rate of the repellent varies regularly over different hanging times. In particular, the repellency rate against pine sawyer beetles reaches 100% within 15 and 30 days after hanging, effectively protecting healthy pine trees within this timeframe. However, after 45 days, the repellency rate in the forest drops significantly, reducing the protective effect. Considering the costs of the agent and labor during use, this invention selects 30 days as the optimal application time for the repellent.

[0140] In summary, the repellent should be placed 10-20 meters away from the pine sawyer beetle trap, and the repellent should be replaced every 30 days. This will achieve a 100% repellency rate against the pine sawyer beetle, meaning that there will be no adult pine sawyer beetle activity within this range and time period. This will provide a more economical and effective protection for healthy pine trees.

Claims

1. A method for applying a repellent to effectively protect pine trees from pine wilt disease, characterized in that: Includes the following steps: The forest is divided into circular areas, thus establishing multiple circular areas; Place a repellent agent at the center point of each circular area; Within each circular area, starting from the repellent placed at the center point, pine beetle traps are set in a direction that spreads outward from the center to the circumference of the circular area, and the pine beetles are lured into the traps. The distance between the repellent and the pine beetle trap is 0-40 meters. The pine beetle trap contains a pine beetle attractant. The repellent comprises the following raw material components in parts by weight: 10-30 parts of 4-allyl anisole, 10-30 parts of beta-benzyl acrolein, 60-80 parts of ethanol, 2-8 parts of Tween-80, 10-20 parts of linalool, and 30-50 parts of coating agent. The coating agent comprises the following raw material components in parts by weight: 30-50 parts gelatin, 5-15 parts quaternized chitosan, 7-13 parts glycerol, 1.6-2.5 parts linalool, 0.45-0.6 parts Tween-80, and 10-15 parts rice starch; The preparation method of the coating agent includes the following steps: Gelatin and quaternized chitosan were mixed, glycerol was added and mixed, then the first portion of linalool was added and mixed, and finally Tween-80 was added and mixed to obtain a complex. Mix rice starch with water, heat at 90-100℃ for 1-2 hours, cool down to 20-30℃, add the second part of linalool and mix, then heat to 30-50℃, add the complex and mix to obtain the coating agent. The mass ratio of the first part of linalool to the second part of linalool is (1-2):(0.1-1.5).

2. The method for applying a repellent to effectively protect pine trees from pine wilt disease according to claim 1, characterized in that: The distance between the repellent and the pine beetle trap is 0-20 meters.

3. The method for applying a repellent to effectively protect pine trees from pine wilt disease according to claim 1, characterized in that: The repellent should be replaced periodically, following these steps: replace the repellent every 15-45 days.

4. The method for applying a repellent to effectively protect pine trees from pine wilt disease according to claim 3, characterized in that: The repellent should be replaced periodically, following these steps: replace the repellent every 15-30 days.

5. The method for applying a repellent to effectively protect pine trees from pine wilt disease according to claim 1, characterized in that: The repellent also includes 10-16 parts by weight of eugenol.

6. The method for applying a repellent to effectively protect pine trees from pine wilt disease according to claim 5, characterized in that: The repellent also includes 5-15 parts by weight of benzoyl acetate and 5-15 parts by weight of vanillin.

7. The method for applying a repellent to effectively protect pine trees from pine wilt disease according to claim 6, characterized in that: The repellent also includes 3.5-4.5 parts by weight of stearic acid and 2.5-3.5 parts by weight of isopropyl palmitate.

Images