Efficient high-yield cultivation method of cinnamomum subavenium

Abstract

The application discloses a high-efficiency high-yield cultivation method of Cinnamomum lebrunii, belongs to the technical field of Cinnamomum lebrunii cultivation, and through the treatment of top pruning and branch pulling on Cinnamomum lebrunii plants at specific times and the application of growth promoters for promoting root growth of the Cinnamomum lebrunii plants and regulating nutrient absorption at different stages, the Cinnamomum lebrunii plants are jointly promoted to form more branches, the tree body morphology is transformed to be short, strong and thick, the branch and leaf growth of the Cinnamomum lebrunii is improved, and the production of old eagle tea is improved, so that the high-efficiency high-yield cultivation method has a good application prospect in the field of old eagle tea production.

Description

Technical Field

[0001] This invention relates to the field of camphor tree cultivation technology, and in particular to a high-efficiency and high-yield cultivation method for camphor tree. Background Technology

[0002] Cinnamomum camphora, a small tree belonging to the genus Litsea in the family Lauraceae. Eagle tea, made from the fresh leaves of Cinnamomum camphora, has been a popular beverage since ancient times, possessing health benefits such as quenching thirst, clearing heat, and detoxifying. It is highly favored in the market, demonstrating significant application and economic value. The widening market supply-demand gap and increasing health awareness have also accelerated the rapid development of the Cinnamomum camphora industry.

[0003] Eagle Tea production relies on harvesting the tender leaves of the *Cinnamomum camphora*. The primary purpose of cultivating *Cinnamomum camphora* is to obtain high-yield, high-quality fresh leaf raw materials. However, current research on *Cinnamomum camphora* mainly focuses on its morphological characteristics and geographical distribution, chemical composition and pharmacological effects, physiological characteristics, and molecular biology, with less research on how to obtain high-yield, high-quality fresh leaf raw materials. In conventional cultivation, to increase Eagle Tea yield, fertilization is generally used to promote the growth of *Cinnamomum camphora* to obtain more fresh leaves. However, *Cinnamomum camphora* is a species with strong apical dominance; indiscriminate fertilization not only fails to effectively increase Eagle Tea yield but also leads to excessive plant growth, making harvesting difficult, reducing wind resistance, and increasing the plant's susceptibility to lodging.

[0004] Therefore, there is a need to find a high-efficiency and high-yield cultivation method for *Cinnamomum camphora* to effectively increase the yield of *Eagle Tea* and solve the problem that conventional fertilization methods can only increase the yield of *Eagle Tea* and can easily lead to excessive plant growth, making them difficult to harvest and prone to lodging. Summary of the Invention

[0005] Therefore, the purpose of this invention is to provide a high-efficiency and high-yield cultivation method for *Camellia sinensis*, which solves the problem that conventional fertilization methods have limited yield improvement for *Camellia sinensis* and easily lead to excessive plant growth, making them difficult to harvest and prone to lodging.

[0006] The present invention solves the above-mentioned technical problems through the following technical means: A high-efficiency and high-yield cultivation method for *Cinnamomum camphora*, the method comprising the following steps: (1) Seedling planting: In November and December, select 2-3 year old seedlings with a ground diameter of 0.4-0.5 cm and the first layer of branches at a distance of 40-50 cm from the ground for transplanting and planting. Prepare root-setting water with 0.1% methyl thiophanate and potassium humate and water thoroughly after transplanting. (2) Pinching: In July or August of the second year after the seedlings are planted, pinch off the terminal buds of the middle dominant branches at a length of 3-4 cm, leaving 2-3 pairs of buds to cultivate the second layer of lateral branches. The remaining unpinched lateral branches are the first layer of lateral branches. (3) Fertilization: Starting from the second year after transplanting, apply organic fertilizer and compound fertilizer after turning over the soil and removing weeds in November each year. Specifically, the amount of organic fertilizer applied is 2-2.5 kg / plant and the amount of compound fertilizer applied is 100 g / plant. Starting from the third year after transplanting, apply growth promoter at a rate of 1-2 kg / plant in May each year. (4) Branch pulling: In the third year after planting, the first layer of lateral branches grow into main branches and sprout upright branches. In July to September, the branches are pulled to keep the upright branches parallel to the ground. At the same time, the buds retained on the middle dominant branches after the topping in step (2) grow into the second layer of lateral branches and sprout new buds. Then, the terminal bud of the sprouted new buds is pinched off, and the remaining new buds are retained to continue to grow. The second layer of lateral branches are pulled to keep them parallel to the ground. (5) Pruning: When the height of the leopard skin camphor tree reaches 130-140cm and the crown width reaches 50-60cm, the top is removed and leaves are left for light harvesting. The tree is then pruned to ensure that the height of the tree does not exceed 150cm. The number of buds on each inner branch is controlled to be within the range of 4-5, and weak branches and residual branches are removed in time. (6) Harvesting: The tender leaves are harvested every spring to obtain Eagle Tea. When harvesting, the leaves are pinched off 1-2 cm below the leaf.

[0007] Furthermore, in step (1), the row spacing for seedling planting is 1.75m and the plant spacing is 1.5m.

[0008] Furthermore, in step (1), base fertilizer is applied when the seedlings are planted. Fertilizer is applied at the vertical position of the crown, and the fertilizer is mixed evenly with the soil at a rate of 1.5-2 kg / plant. Then, it is applied at a depth of 20-30 cm and a width of 25-40 cm. The base fertilizer is a mixture of well-rotted manure and compound fertilizer at a mass ratio of 20:1. The compound fertilizer contains 15% nitrogen, phosphorus, and potassium.

[0009] Furthermore, the growth promoter in step (3) includes the following raw materials: Sodium alginate, calcium chloride, konjac glucomannan, indolebutyric acid, diammonium hydrogen phosphate, potassium sulfate, n-tetranol, urea, xanthan gum, ethyl glycolate, carbodiimide, N-hydroxysuccinimide, diatomaceous earth, catechol.

[0010] Furthermore, the method for preparing the growth promoter is as follows: A: Add xanthan gum to water and stir to disperse. Then add carbodiimide and N-hydroxysuccinimide. Adjust the pH to 6-7 and stir at room temperature for 30 min. Then dissolve ethyl glycolate in ethanol and add it. Continue stirring to react for 2-6 h. After the reaction is complete, vacuum dry at 40-60℃ and 10-40 mbar for 12 h to obtain modified xanthan gum. B: Add modified xanthan gum to water and stir to disperse. Then add catechol and stir continuously at room temperature for 30 minutes. Then add urea and mix evenly. Adjust the pH to 7, then add diatomaceous earth and stir to mix evenly. Then put it into a granulator to granulate and dry it to constant weight to obtain core layer particles. C: Heat n-tetranol to 40°C and stir to melt it. Then, spray it onto the surface of the core layer particles while it is hot and refrigerate it overnight at 4°C to obtain the isolation layer embedded particles. D: Dissolve sodium alginate in water to prepare a 3wt% sodium alginate solution. Add konjac glucomannan to the sodium alginate solution and mix well. Then add indolebutyric acid, diammonium hydrogen phosphate, and potassium sulfate and stir thoroughly to obtain a suspension. Add the isolation layer-embedded particles to the suspension and stir slowly for 3-5 minutes. Then remove them and place them in a 2wt% calcium chloride solution to allow the surface suspension to gel. After the surface suspension has fully gelled, remove them and place them back into the suspension. Stir slowly for 3-5 minutes and remove them again and place them in a 2wt% calcium chloride solution to allow the surface suspension to gel again. Finally, remove them and wash them with clean water to obtain the growth promoter.

[0011] Furthermore, in step A, the mass ratio of xanthan gum, carbodiimide, N-hydroxysuccinimide, and ethyl glycolate is (0.5-1):(0.01-0.02):(0.02-0.03):(0.05-0.1).

[0012] Furthermore, in step B, the mass ratio of modified xanthan gum, catechol, urea, and diatomaceous earth is (0.5-1):(0.05-0.1):(8-15):(3-5).

[0013] Furthermore, in step B, the particle size of the core layer particles is 4-6 mm.

[0014] Furthermore, in the suspension of step D, the mass ratio of sodium alginate, konjac glucomannan, indolebutyric acid, diammonium hydrogen phosphate, and potassium sulfate is (0.5-1):(0.01-0.02):(0.001-0.002):(2-3):(1-2).

[0015] Plant growth can be divided into two stages: vegetative growth and reproductive growth, generally with flower bud differentiation as the dividing point. Before flower bud differentiation, it is mainly the vegetative growth stage, which focuses on the growth of vegetative organs such as roots, stems, and leaves. After flower bud differentiation, it enters the reproductive growth stage, which focuses on the growth and development of reproductive organs such as flowers, fruits, and seeds. Reproductive growth requires a large amount of nutrients, which compete with the nutrients needed to maintain the continuous growth of vegetative organs such as leaves. Vigorous reproductive growth will preferentially allocate nutrients, thereby inhibiting vegetative growth. In order to promote the growth of branches and leaves of *Cinnamomum camphora* and obtain more high-quality fresh leaves for *Eagle Tea*, this invention, in addition to the management operations such as topping and branch pulling of *Cinnamomum camphora*, also applies a growth promoter to treat *Cinnamomum camphora*. The growth promoter promotes the growth of the root system of *Cinnamomum camphora* and regulates the nutrient absorption between the vegetative and reproductive growth stages, thereby inhibiting reproductive growth and allowing more nutrients to flow to the vegetative growth stage to achieve high-yield cultivation of *Cinnamomum camphora*.

[0016] Since flower bud differentiation is the starting point of reproductive growth, inhibiting flower bud differentiation in *Cinnamomum camphora* can effectively inhibit its later reproductive growth. Phosphorus is a key element for flower bud differentiation, and a lack of phosphorus can inhibit flower bud differentiation in *Cinnamomum camphora*. At the same time, nitrogen is an important nutrient for the growth of *Cinnamomum camphora*, but high nitrogen content during the flower bud differentiation stage will inhibit flower bud differentiation. This invention reduces the supply of phosphorus during the flower bud differentiation period by applying growth promoters and creates a high-nitrogen environment to inhibit flower bud differentiation, thereby achieving the purpose of inhibiting later reproductive growth.

[0017] Specifically, the outermost layer of the growth promoter is made of sodium alginate gel loaded with indolebutyric acid, diammonium hydrogen phosphate, and potassium sulfate. When the growth promoter is applied to the soil, the diammonium hydrogen phosphate loaded in the sodium alginate gel releases hydrogen phosphate ions, which compete with the sodium alginate gel for calcium ions. This causes the sodium alginate gel structure formed by calcium ion cross-linking to disintegrate, rapidly releasing the loaded indolebutyric acid, diammonium hydrogen phosphate, and potassium sulfate. Indolebutyric acid, combined with other components, promotes the root growth of *Cinnamomum camphora*. Strong roots can absorb more water and nutrients, promote photosynthesis in the above-ground leaves, and increase dry matter accumulation. Sufficient photosynthetic products will provide the necessary nutrients for root growth and development. The two complement each other, effectively promoting the vegetative growth of *Cinnamomum camphora*. At the same time, a strong root system can significantly improve the lodging resistance of *Cinnamomum camphora*.

[0018] In July, *Cinnamomum camphora* enters the flower bud differentiation stage and then begins reproductive growth. This coincides with summer, when temperatures rise. The outermost layer of the growth promoter decomposes and releases, exposing the insulating layer containing particles. Due to the high soil temperature, the tridecanoic acid in the insulating layer reaches its melting point and melts away, exposing the core particles. These core particles release a high amount of urea embedded within them, creating a high-nitrogen environment for the *Cinnamomum camphora* roots. This high-nitrogen environment inhibits flower bud differentiation. Furthermore, this invention adds catechol to treat the xanthan gum containing urea in the core particles. By adjusting its surface chemical properties, it promotes the adsorption and fixation of phosphorus in acidic soil by xanthan gum, thereby inhibiting phosphorus absorption during flower bud differentiation. The combined effect of the high-nitrogen and phosphorus-deficient environments inhibits flower bud differentiation in *Cinnamomum camphora*, thus suppressing later reproductive growth.

[0019] To ensure the timely release of urea embedded in the core layer particles and to create a high-nitrogen environment to inhibit flower bud differentiation in *Cinnamomum camphora*, this invention uses ethyl glycolate to treat xanthan gum to obtain modified xanthan gum, which then embeds urea. Ethyl glycolate reacts and binds to the xanthan gum molecular chain, introducing acid-sensitive ester bonds. When the isolation layer of n-tetranol melts and is lost, the core layer particles come into contact with the acidic soil of *Cinnamomum camphora*, promoting the hydrolysis and bond breaking of the ester bonds, thereby promoting the rapid chain breaking and decomposition of xanthan gum, allowing it to release urea in a timely manner to form a high-nitrogen environment. Beneficial effects

[0020] This invention describes a specific method for removing terminal buds and branching of *Cinnamomum camphora* seedlings, combined with the application of growth promoters that regulate nutrient supply and absorption at different stages of plant growth. This alters the original branching habits of the plant, thereby increasing the number of effective branches, promoting a rational branching pattern, maximizing the canopy area, and efficiently promoting vegetative growth. The result is a short, strong, and wide tree, effectively increasing the yield of *Cinnamomum camphora* tea and showing promising application prospects in the production of Lao Ying tea. Attached Figure Description

[0021] Figure 1 : This is a picture showing the growth status of camphor trees cultivated in experimental group 1 of this invention. Detailed Implementation

[0022] The present invention will now be described in detail with reference to specific embodiments and accompanying drawings: Example 1: Preparation of growth promoter A: Add 0.7 kg xanthan gum to 14 kg of water and stir to disperse. Then add 0.015 kg carbodiimide and 0.025 kg N-hydroxysuccinimide, adjust the pH to 6.5, and stir at room temperature for 30 min. Then dissolve 0.07 kg ethyl glycolate in 0.7 kg of ethanol and add it to the mixture. Continue stirring for 4 h. After the reaction is complete, vacuum dry at 50 °C and 25 mbar for 12 h to obtain modified xanthan gum. B: Add 0.7 kg of modified xanthan gum to 14 kg of water and stir to disperse. Then add 0.07 kg of catechol and stir continuously at room temperature for 30 min. Then add 12 kg of urea and mix evenly. Adjust the pH to 7, then add 4 kg of diatomaceous earth and stir to mix evenly. Then put it into a granulator to prepare particles with a particle size of about 5 mm and dry them to constant weight to obtain the core layer particles. C: Heat n-tetranol to 40°C and stir to melt it. Then spray it onto the surface of the core layer particles while it is hot. The amount of spraying is 2% of the mass of the core layer particles. After spraying, immediately transfer it to 4°C and refrigerate it overnight to obtain the isolation layer embedded particles. D: Dissolve 0.7 kg of sodium alginate in water to prepare a 3 wt% sodium alginate solution. Add 0.015 kg of konjac glucomannan to the sodium alginate solution and mix well. Then add 0.0015 kg of indolebutyric acid, 2.5 kg of diammonium hydrogen phosphate, and 1.5 kg of potassium sulfate and stir thoroughly to obtain a suspension. Add the isolation layer-embedded particles to the suspension and stir slowly for 4 minutes. Then remove them and place them in a 2 wt% calcium chloride solution to allow the surface suspension to gel. After the surface suspension has fully gelled, remove them and place them back into the suspension. Stir slowly for 4 minutes and remove them again and place them in a 2 wt% calcium chloride solution to allow the surface suspension to gel again. Finally, remove them and wash them with clean water to obtain the growth promoter.

[0023] Example 2: Preparation of growth promoter II A: Add 0.5 kg xanthan gum to 10 kg of water and stir to disperse. Then add 0.01 kg carbodiimide and 0.02 kg N-hydroxysuccinimide. Adjust the pH to 6 and stir at room temperature for 30 min. Then dissolve 0.05 kg ethyl glycolate in 0.5 kg of ethanol and add it to the mixture. Continue stirring for 2 h. After the reaction is complete, vacuum dry at 40 °C and 10 mbar for 12 h to obtain modified xanthan gum. B: Add 0.5 kg of modified xanthan gum to 10 kg of water and stir to disperse. Then add 0.05 kg of catechol and stir continuously at room temperature for 30 min. Then add 8 kg of urea and mix evenly. Adjust the pH to 7, then add 3 kg of diatomaceous earth and stir to mix evenly. Then put it into a granulator to prepare particles with a particle size of about 5 mm and dry to constant weight to obtain core layer particles. C: Heat n-tetranol to 40°C and stir to melt it. Then spray it onto the surface of the core layer particles while it is hot. The amount of spraying is 2% of the mass of the core layer particles. After spraying, immediately transfer it to 4°C and refrigerate it overnight to obtain the isolation layer embedded particles. D: Dissolve 0.5 kg of sodium alginate in water to prepare a 3 wt% sodium alginate solution. Add 0.01 kg of konjac glucomannan to the sodium alginate solution and mix well. Then add 0.001 kg of indolebutyric acid, 2 kg of diammonium hydrogen phosphate, and 1 kg of potassium sulfate and stir thoroughly to obtain a suspension. Add the isolation layer-embedded particles to the suspension and stir slowly for 3 minutes. Then remove them and place them in a 2 wt% calcium chloride solution to allow the surface suspension to gel. After the surface suspension has fully gelled, remove them and place them back into the suspension. Stir slowly for 3 minutes and remove them again and place them in a 2 wt% calcium chloride solution to allow the surface suspension to gel again. Finally, remove them and wash them with clean water to obtain the growth promoter.

[0024] Example 3: Preparation of growth promoters A: Add 1 kg xanthan gum to 20 kg of water and stir to disperse. Then add 0.02 kg carbodiimide and 0.03 kg N-hydroxysuccinimide. Adjust the pH to 7 and stir at room temperature for 30 min. Then dissolve 0.1 kg ethyl glycolate in 1 kg of ethanol and add it. Continue stirring for 6 h. After the reaction is complete, vacuum dry at 60 °C and 40 mbar for 12 h to obtain modified xanthan gum. B: Add 1 kg of modified xanthan gum to 20 kg of water and stir to disperse. Then add 0.1 kg of catechol and stir continuously at room temperature for 30 min. Then add 15 kg of urea and mix evenly. Adjust the pH to 7, then add 5 kg of diatomaceous earth and stir to mix evenly. Then put it into a granulator to prepare particles with a particle size of about 5 mm and dry to constant weight to obtain core layer particles. C: Heat n-tetranol to 40°C and stir to melt it. Then spray it onto the surface of the core layer particles while it is hot. The amount of spraying is 2% of the mass of the core layer particles. After spraying, immediately transfer it to 4°C and refrigerate it overnight to obtain the isolation layer embedded particles. D: Dissolve 1 kg of sodium alginate in water to prepare a 3 wt% sodium alginate solution. Add 0.02 kg of konjac glucomannan to the sodium alginate solution and mix well. Then add 0.002 kg of indolebutyric acid, 3 kg of diammonium hydrogen phosphate, and 2 kg of potassium sulfate and stir thoroughly to obtain a suspension. Add the isolation layer-embedded particles to the suspension and stir slowly for 5 minutes. Then remove them and place them in a 2 wt% calcium chloride solution to allow the surface suspension to gel. After the surface suspension has fully gelled, remove them and place them back into the suspension. Stir slowly for 5 minutes and remove them again and place them in a 2 wt% calcium chloride solution to allow the surface suspension to gel again. Finally, remove them and wash them with clean water to obtain the growth promoter.

[0025] Comparative Example 1: Preparation of Growth Promoter Compared with Example 1, the only difference is that the original step A was omitted in the preparation of the growth promoter in Comparative Example 1, that is, no modification treatment was performed on xanthan gum, but conventional xanthan gum was used, as shown below: A: Add 0.7 kg xanthan gum to 14 kg of water and stir to disperse. Then add 0.07 kg catechol and stir continuously at room temperature for 30 min. Then add 12 kg urea and mix evenly. Adjust the pH to 7, then add 4 kg diatomaceous earth and stir to mix evenly. Then put it into a granulator to prepare particles with a particle size of about 5 mm and dry them to constant weight to obtain the core layer particles. B: Same as step C in Example 1; C: Same as step D in Example 1.

[0026] Comparative Example 2: Preparation of Growth Promoter Compared with Example 1, the only difference is that catechol was not added in step B of the preparation of the growth promoter in Comparative Example 2, while the other steps were the same as in Example 1.

[0027] Comparative Example 3: Preparation of Growth Promoter Compared with Example 1, the only difference is that in Comparative Example 3, the original step C was omitted in the preparation of the growth promoter. Instead of using n-tetranol to treat the core layer particles, the core layer particles were directly added to the suspension to prepare the growth promoter. All other steps were the same as in Example 1.

[0028] Comparative Example 4: Preparation of Growth Promoter Compared with Example 1, the only difference is that in Comparative Example 4, the original step D was omitted in the preparation of the growth promoter, that is, the particles were directly embedded in the isolation layer as the growth promoter. All other steps are the same as in Example 1.

[0029] Comparative Example 5: Preparation of Growth Promoter In contrast to Example 1, the only difference is that in step B of Comparative Example 5, the pH was adjusted to 6; all other steps were the same as in Example 1.

[0030] Comparative Example 6: Preparation of Growth Promoter Compared with Example 1, the only difference is that in Comparative Example 6, the pH was adjusted to 8 in step B during the preparation of the growth promoter; all other steps were the same as in Example 1.

[0031] Comparative Example 7: Preparation of Growth Promoter In contrast to Example 1, the difference lies in the preparation of the growth promoters in Comparative Example 7, where isolation layer-embedded particles and sodium alginate-embedded particles were prepared as growth promoter A and growth promoter B, respectively, as detailed below: A-B: Same as in Example 1; C: Heat n-tetranol to 40°C and stir to melt it. Then, while still hot, spray it onto the surface of the core layer particles. The spraying amount is 2% of the mass of the core layer particles. After spraying, immediately transfer it to 4°C and refrigerate overnight to obtain the isolation layer embedded particles, which is the growth promoter A. D: Dissolve 0.7 kg of sodium alginate in water to prepare a 3 wt% sodium alginate solution. Add 0.015 kg of konjac glucomannan to the sodium alginate solution and mix well. Then add 0.0015 kg of indolebutyric acid, 2.5 g of diammonium hydrogen phosphate and 1.5 kg of potassium sulfate and stir thoroughly to obtain a suspension. Add the suspension dropwise to a 2 wt% calcium chloride solution for gelation. Then remove the suspension and wash it with water to obtain sodium alginate-encapsulated particles, which is growth promoter B.

[0032] Example 4: High-yield cultivation method of Cinnamomum camphora. (1) Seedling planting: In November, select 2-year-old seedlings with a ground diameter of about 0.4 cm and the first layer of branches about 40 cm from the ground for transplanting. The row spacing for transplanting is 1.75 m and the plant spacing is 1.5 m. When transplanting, base fertilizer should be applied. Specifically, mix well-rotted manure and compound fertilizer in a mass ratio of 20:1 to obtain base fertilizer. After mixing the base fertilizer with the soil, apply it at a depth of about 25 cm and a width of about 30 cm at the vertical position of the crown. The amount applied is about 1.5 kg / plant. Weigh methyl thiophanate and add it to water to prepare a 0.1% methyl thiophanate solution. Then add 50% of the mass of methyl thiophanate potassium humate and mix well to obtain root water. Water thoroughly after transplanting the seedlings. (2) Pinching: In July of the second year after planting, pinch off the terminal bud of the dominant branch in the middle at a length of about 4cm, leaving 2 to 3 pairs of buds to cultivate the second layer of lateral branches. The remaining unpinched lateral branches are the first layer of lateral branches. (3) Fertilization: Starting from the second year after transplanting, apply organic fertilizer and compound fertilizer after turning over the soil and removing weeds in November each year. Specifically, the amount of organic fertilizer applied is 2 kg / plant and the amount of compound fertilizer applied is 100 g / plant. Starting from the third year after transplanting, apply the growth promoter prepared according to the method in Example 1 in May each year at a rate of 1 kg / plant. (4) Branch pulling: In the third year after planting, the first layer of lateral branches grow into main branches and sprout upright branches. In July, the branches are pulled to keep the upright branches parallel to the ground. At the same time, the buds retained on the middle dominant branches after the topping in step (2) grow into the second layer of lateral branches and sprout new buds. Then, the terminal bud of the sprouted new buds is pinched off, and the remaining new buds are retained to continue to grow. The second layer of lateral branches are pulled to keep them parallel to the ground. (5) Pruning: When the height of the leopard skin camphor reaches 130cm and the crown width is 50cm, lightly harvest the top leaves and prune the plant to ensure that the plant height does not exceed 150cm, control the number of buds on each inner branch to within 5, and promptly remove weak branches and dead branches. (6) Harvesting: The tender leaves are harvested every spring to obtain Eagle Tea. When harvesting, the leaves are pinched off 1-2 cm below the leaf.

[0033] Experiment: Cultivation Experiment of Camphor Tree with Leopard Skin 1. A cultivation experiment of *Cinnamomum camphora* was conducted at the Chongqing Forestry Science Research Institute Experimental Base in Gaodianzi, Gele Mountain, Shapingba District, Chongqing. The experiment was divided into 9 groups: experimental group 1, control groups 1-7, and blank control group. The cultivation methods for each group are as follows: Experimental group 1: The growth promoter prepared in Example 1 and the cultivation method of Example 4 were used; Control groups 1-7: The growth promoters of comparative examples 1-7 and the cultivation method of example 4 were used respectively. In control group 7, growth promoter B was applied in May and growth promoter A was applied in July. The application rate of growth promoter A was 0.3 kg / plant and the application rate of growth promoter B was 0.7 kg / plant. Blank control group: No growth promoter was used, and the remaining methods and steps were the same as in Example 4.

[0034] Each group used seedlings with a ground diameter of 0.4 cm and consistent growth of the first layer of branches at about 40 cm above the ground as experimental seedlings. Each group had 8 experimental plants. Then, in the fourth year after planting, the yield (fresh weight) of tea plants in each group was recorded, and the data are shown in Table 1.

[0035] Table 1

[0036] Based on the data analysis in Table 1, we can conclude that: (1) Cultivating camphor trees according to the method of the present invention can effectively increase the yield of eagle tea. In experimental group 1, the fresh weight of a single camphor tree can reach 1.84 kg / tree; compared with the blank control group, the yield per tree increased by 0.37 kg / tree.

[0037] (2) In control group 1, xanthan gum was not modified during the preparation of the growth promoter, but conventional xanthan gum was used. As a urea embedding material, xanthan gum has good acid resistance. Therefore, the release of nitrogen during the later flower bud differentiation period was not timely, which affected the inhibitory effect on reproductive growth and further affected the growth of branches and leaves, resulting in a decrease in yield. In control group 2, catechol was not added during the preparation of the growth promoter, which failed to effectively adsorb and fix phosphorus. Consequently, the absorption and utilization of phosphorus during the reproductive growth stage was promoted, which reduced vegetative growth and resulted in a decrease in tea yield. In control group 3, n-tetaneol was not added during the preparation of the growth promoter, which had poor isolation performance for the inner layer of urea. This led to the early release of urea, which affected the creation of a high-nitrogen environment in the later stage. The inhibitory effect on reproductive growth was poor, which affected the vegetative growth of branches and leaves and resulted in a decrease in yield. (3) In control group 4, the particles embedded in the isolation layer were used directly as growth promoters. The lack of diammonium hydrogen phosphate and potassium sulfate promoted vegetative growth. At the same time, the lack of sodium alginate gel embedding layer inhibited the release of urea in the particles embedded in the isolation layer. Vegetative growth was poor and the inhibitory effect on reproductive growth was not obvious, resulting in a serious reduction in yield. In control group 5, the pH was adjusted to 6 in step B, and in control group 6, the pH was adjusted to 8 in step B. The acidic and alkaline environments caused the degradation of the modified xanthan gum of the core layer particle embedding material, and the premature release of urea reduced the inhibitory effect on reproductive growth, thereby affecting the growth of branches and leaves and reducing yield. (4) In control group 7, the growth promoter was prepared into isolation layer embedded particles and sodium alginate particles and applied at the corresponding time. The yield of eagle tea was not significantly different from that of experimental group 1, but the application of growth promoter was increased, which increased labor costs.

[0038] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the present invention, and all such modifications and substitutions should be covered within the scope of the claims of the present invention. Technical aspects, shapes, and structures not described in detail in this invention are all well-known technologies.

Claims

1. A high-efficiency and high-yield cultivation method for *Cinnamomum camphora*, characterized in that, The method includes the following steps: (1) Planting of seedlings; (2) Topping; (3) Fertilization: Starting from the second year after transplanting, apply organic fertilizer and compound fertilizer after turning over the soil and removing weeds in November each year. Specifically, the amount of organic fertilizer applied is 2-2.5 kg / plant and the amount of compound fertilizer applied is 100 g / plant. Starting from the third year after transplanting, apply growth promoter at a rate of 1-2 kg / plant in May each year. (4) Branch pulling; (5) Pruning; (6) Picking; The growth promoter in step (3) includes the following raw materials: Sodium alginate, calcium chloride, konjac glucomannan, indolebutyric acid, diammonium hydrogen phosphate, potassium sulfate, n-tetranol, urea, xanthan gum, ethyl glycolate, carbodiimide, N-hydroxysuccinimide, diatomaceous earth, catechol; The method for preparing the growth promoter is as follows: A: Add xanthan gum to water and stir to disperse. Then add carbodiimide and N-hydroxysuccinimide. Adjust the pH to 6-7 and stir at room temperature for 30 min. Then dissolve ethyl glycolate in ethanol and add it. Continue stirring to react for 2-6 h. After the reaction is complete, vacuum dry at 40-60℃ and 10-40 mbar for 12 h to obtain modified xanthan gum. B: Add modified xanthan gum to water and stir to disperse. Then add catechol and stir continuously at room temperature for 30 minutes. Then add urea and mix evenly. Adjust the pH to 7, then add diatomaceous earth and stir to mix evenly. Then put it into a granulator to granulate and dry it to constant weight to obtain core layer particles. C: Heat n-tetranol to 40°C and stir to melt it. Then, spray it onto the surface of the core layer particles while it is hot and refrigerate it overnight at 4°C to obtain the isolation layer embedded particles. D: Dissolve sodium alginate in water to prepare a 3wt% sodium alginate solution. Add konjac glucomannan to the sodium alginate solution and mix well. Then add indolebutyric acid, diammonium hydrogen phosphate, and potassium sulfate and stir thoroughly to obtain a suspension. Add the isolation layer-embedded particles to the suspension and stir slowly for 3-5 minutes. Then remove them and place them in a 2wt% calcium chloride solution to allow the surface suspension to gel. After the surface suspension has fully gelled, remove them and place them back into the suspension. Stir slowly for 3-5 minutes and remove them again and place them in a 2wt% calcium chloride solution to allow the surface suspension to gel again. Finally, remove them and wash them with clean water to obtain the growth promoter.

2. The method for high-efficiency and high-yield cultivation of *Cinnamomum camphora* according to claim 1, characterized in that, In step (1), the row spacing for seedling planting is 1.75m and the plant spacing is 1.5m. The specific steps for seedling planting, topping, branch pulling, pruning, and harvesting are as follows: Seedling planting: In November and December, select 2-3 year old seedlings with a ground diameter of 0.4-0.5 cm and the first layer of branches at a distance of 40-50 cm from the ground for transplanting. Prepare a root-setting water solution of 0.1% methyl thiophanate and potassium humate and water it thoroughly after transplanting. Pinching: In July or August of the second year after the seedlings are planted, pinch off the terminal buds of the dominant branches in the middle at a length of 3-4 cm, leaving 2-3 pairs of buds to cultivate the second layer of lateral branches. The remaining unpinched lateral branches are the first layer of lateral branches. Branch pulling: In the third year after planting, the first layer of lateral branches grow into main branches and sprout upright branches. In July to September, the branches are pulled to keep the upright branches parallel to the ground. At the same time, the buds retained on the middle dominant branches after the topping in step (2) grow into the second layer of lateral branches and sprout new buds. Then pinch off the terminal bud of the sprouted new buds, retain the remaining new buds to continue to grow, and pull the second layer of lateral branches to keep them parallel to the ground. Pruning: When the height of the camphor tree reaches 130-140cm and the crown width reaches 50-60cm, lightly prune the top, leaving leaves, and prune the plant to ensure that the height of the plant does not exceed 150cm. Control the number of buds on each inner branch to within the range of 4-5, and promptly remove weak and damaged branches. Harvesting: The tender leaves are harvested every spring to obtain Eagle Tea. When harvesting, the leaves are pinched off 1-2 cm below the leaf.

3. The method for high-efficiency and high-yield cultivation of *Cinnamomum camphora* according to claim 2, characterized in that, In step (1), base fertilizer needs to be applied when the seedlings are planted. Fertilizer should be applied at the vertical position of the crown. The fertilizer should be mixed evenly with the soil at a rate of 1.5-2 kg / plant, and then applied at a depth of 20-30 cm and a width of 25-40 cm. The base fertilizer is a mixture of well-rotted manure and compound fertilizer at a mass ratio of 20:

1. The compound fertilizer contains 15% nitrogen, phosphorus and potassium.

4. The method for high-efficiency and high-yield cultivation of *Cinnamomum camphora* according to claim 3, characterized in that, In step A, the mass ratio of xanthan gum, carbodiimide, N-hydroxysuccinimide, and ethyl glycolate is (0.5-1):(0.01-0.02):(0.02-0.03):(0.05-0.1).

5. The method for high-efficiency and high-yield cultivation of *Cinnamomum camphora* according to claim 4, characterized in that, In step B, the mass ratio of modified xanthan gum, catechol, urea, and diatomaceous earth is (0.5-1):(0.05-0.1):(8-15):(3-5).

6. The method for high-efficiency and high-yield cultivation of *Cinnamomum camphora* according to claim 5, characterized in that, In step B, the particle size of the core layer particles is 4-6 mm.

7. The method for high-efficiency and high-yield cultivation of *Cinnamomum camphora* according to claim 6, characterized in that, In step D, the mass ratio of sodium alginate, konjac glucomannan, indolebutyric acid, diammonium hydrogen phosphate, and potassium sulfate in the suspension is (0.5-1):(0.01-0.02):(0.001-0.002):(2-3):(1-2).

Images