Object recognition device and method for detecting cognitive function of vascular cognitive impairment rats

Abstract

The application relates to an object recognition device and method for detecting the cognitive function of a vascular cognitive impairment rat, which comprises a mounting column, the lower end of the mounting column is fixed with a bottom plate, and the upper end of the mounting column is fixed with a top plate; a new object recognition box is arranged on the upper surface of the bottom plate, a movable door is arranged on one side of the new object recognition box, a rotating disc and a rotating mechanism are arranged on the mounting column, two telescopic units are arranged on the rotating disc, a group of new objects are arranged at the front end of the telescopic units, and only one common new object is contained between the two groups of new objects; a temporary breeding cage and a fixing groove are arranged on the top plate, a through hole is formed in the bottom of the fixing groove, a plurality of iron pieces are arranged around the edge of the floor, a plurality of magnets corresponding to the adsorbed iron pieces are arranged on the cage body, and a camera is detachably arranged on the inner top of the cage body; full-automatic operation of the movement, cleaning and disinfection, and switching of new objects of the rat during the experiment can be realized, manual operation is not needed, and the intervention operation caused by human beings is reduced.

Description

Technical Field

[0001] This invention relates to the field of vascular cognitive technology, and more specifically, to an object recognition device and method for detecting cognitive function in rats with vascular cognitive impairment. Background Technology

[0002] The test for detecting new object recognition in vascular cognition involves administering medication to animals and conducting a new object recognition experiment to assess the cognitive memory level of rats. An animal motion tracking system is used to track the nose tip of the rats in detail to achieve the new object recognition experiment, automatically measuring and analyzing a large number of parameters. In the new object recognition ability test, the insertion and removal of new and familiar objects by animals is measured and compared. The greatest advantage of this experiment is that it allows animals to conduct learning and memory tests in a completely natural state, better simulating the learning and memory behavior of humans and primates.

[0003] Experiments are typically divided into three phases:

[0004] Phase 1:

[0005] 1. Gently remove the laboratory animal from its cage and place it in a corner of a new object recognition box where there are no other objects, allowing it to explore freely.

[0006] 2. After the rats have adapted for 10 minutes, record their numbers and return them to their original cages.

[0007] 3. Before conducting the next rat experiment, spray the new object recognition box with 75% alcohol and water, and wipe it with paper towels to eliminate odors.

[0008] Phase Two:

[0009] 1. The second phase will take place one day after the first phase.

[0010] 2. Place two identical objects on the ground 5cm away from the wall of the experimental setup box, and fix their bottoms with double-sided tape to prevent them from moving.

[0011] 3. Gently remove the experimental animal from its cage and place it in the corner of the new object recognition box, allowing the rat to explore freely for 5 minutes.

[0012] 4. After the time is up, remove the rats to a temporary rearing cage.

[0013] Spray the new object recognition box with 5.75% alcohol and water, and then wipe it with paper towels to eliminate odors.

[0014] Phase Three:

[0015] 1. The third stage can begin one hour after the end of the second stage. The longer the stage, the greater the demands on the rat's memory.

[0016] 2. Replace one of the objects from the second stage with a new object and place it on the ground 5cm away from the wall of the experimental setup box. Secure the bottom with double-sided tape to prevent movement.

[0017] 3. Gently remove the experimental animal from its cage and place it in the corner of the new object recognition box, allowing the rat to explore freely for 5 minutes.

[0018] 4. The experimenter moves to a location where the rat is not visible and clicks to record the video. The recording time is 5 minutes, and the recorded video is saved.

[0019] 5. After the time is up, remove the rats to a temporary rearing cage.

[0020] Spray the new object recognition box with 6.75% alcohol and water, and then wipe it with paper towels to eliminate odors.

[0021] The experiment requires repeated handling of rats, disinfection of the new object recognition box, and fixing of the new object. Currently, these actions are all performed manually, which is labor-intensive and prone to errors due to human factors, affecting the final experimental results. A more intelligent object recognition device and method for detecting the cognitive function of rats with vascular cognitive impairment is needed. Summary of the Invention

[0022] The technical problem to be solved by the present invention is to provide an object recognition device for detecting the cognitive function of rats with vascular cognitive impairment, and to provide a method for detecting the cognitive function of rats with vascular cognitive impairment, in order to address the above-mentioned deficiencies of the prior art.

[0023] The technical solution adopted by this invention to solve its technical problem is:

[0024] An object recognition device for detecting cognitive function in rats with vascular cognitive impairment is constructed, comprising a mounting column, a base plate fixed to the lower end of the mounting column, and a top plate fixed to the upper end of the mounting column; a new object recognition box is disposed on the upper surface of the base plate, and a movable door is disposed on the side of the new object recognition box near the mounting column; a turntable and a rotating mechanism for rotating the turntable are disposed on the mounting column; two telescopic units are disposed on the turntable, and a set of new objects is disposed at the front end of the telescopic units, with only one common new object between the two sets of new objects; a temporary feeding cage and a fixing groove for fixing the temporary feeding cage are disposed on the top plate, and a through hole is opened at the bottom of the fixing groove; multiple [unclear text - possibly related to the temporary feeding cage] are arranged around the edge of the floor of the temporary feeding cage. The cage contains several iron components, and multiple magnets are installed on the cage body to attract these components. The floor can pass longitudinally through the through-hole, and a camera is detachably installed on the inner top of the cage body. A floor replacement unit is installed on the top plate to replace the floor. The bottom of the new object recognition box is hollowed out, and the bottom plate is equipped with a movable plate corresponding to the new object recognition box and a lifting unit that drives the movable plate to rise and fall. Electromagnets are installed on the movable plate to attract multiple iron components. An alcohol atomizing unit and a first nozzle assembly and a second nozzle assembly connected to the alcohol atomizing unit are installed on the mounting column. The first nozzle assembly is used to spray the new object, and the second nozzle assembly is partially installed on the movable plate to spray the inner wall of the new object recognition box.

[0025] The object recognition device for detecting cognitive function in rats with vascular cognitive impairment according to the present invention includes a movable groove on the lower end surface of the temporary feeding cage, both ends of which are connected to the outer surface of the cage; a floor is movably disposed within the movable groove; a plurality of magnets are embedded in the inner wall of the movable groove; and a plurality of iron pieces are longitudinally inserted through both ends of the floor.

[0026] The object recognition device for detecting cognitive function in rats with vascular cognitive impairment according to the present invention includes a floor replacement unit comprising a stacking cylinder for stacking floorboards disposed on a top plate, a replacement slot communicating with the fixed slot on the top plate, and the stacking cylinder mounted on the replacement slot; the floor replacement unit further includes a first cylinder for pushing the bottommost floorboard of the stack into a movable slot to replace the current floorboard.

[0027] The object recognition device for detecting cognitive function in rats with vascular cognitive impairment according to the present invention includes a receiving box for receiving replaced floorboards on the top plate.

[0028] The object recognition device for detecting cognitive function in rats with vascular cognitive impairment according to the present invention includes a telescopic unit comprising a second cylinder, the movable end of which is provided with a horizontal plate for mounting a set of new objects; the inner wall of the new object recognition box is provided with a slot that cooperates with the movable door, the upper end of the movable door is rotatably connected to the inner top of the slot via a rotating shaft, and a torsion spring is sleeved on the rotating shaft.

[0029] The object recognition device for detecting cognitive function in rats with vascular cognitive impairment according to the present invention includes a lifting unit comprising a third cylinder, wherein the movable end of the third cylinder is fixed with the movable plate.

[0030] The object recognition device for detecting cognitive function in rats with vascular cognitive impairment according to the present invention includes a second nozzle assembly comprising a multi-port tube, wherein a solenoid valve is provided on the main pipe of the multi-port tube and is connected to the mist outlet of the alcohol atomizing unit, and the sub-tubes of the multi-port tube are arranged in a ring on the bottom surface of the movable plate; the electromagnet is fixed on the surface of the movable plate.

[0031] A method for detecting object recognition in rats with vascular cognitive impairment, using the object recognition device described above, wherein the method includes the following steps:

[0032] Phase 1:

[0033] Rats were placed in temporary feeding cages, and then the temporary feeding cages were placed in the fixing slots on the top plate to fix the cages.

[0034] The lifting unit raises the movable plate until it is in contact with the lower surface of the floor of the temporary feeding cage. The electromagnet is energized to attract iron parts. Then the lifting unit lowers the movable plate to reset, completing the operation of moving the rat into the new object recognition box. The electromagnet is de-energized, and the rat is allowed to explore freely after 10 minutes.

[0035] Once the familiarization time is up, the lifting unit drives the movable plate to rise to the magnet on the cage body to attract the iron parts. Then the lifting unit drives the movable plate to fall back to its original position, completing the operation of removing the rat from the new object recognition box.

[0036] The alcohol atomization unit operates, and the second nozzle assembly is activated to disinfect and deodorize the new object recognition box.

[0037] Phase Two:

[0038] The floor replacement unit replaces the flooring of temporary rearing cages;

[0039] The lifting unit raises the movable plate until it is in contact with the lower surface of the floor of the temporary feeding cage. The electromagnet is energized to attract iron parts. Then the lifting unit lowers the movable plate to reset, completing the operation of moving the rat into the new object recognition box. The electromagnet is then de-energized.

[0040] The telescopic unit operates, pushing a group of new objects through the movable door and extending them into the open space 5cm away from the inner wall of the new object recognition box;

[0041] Allow the rats to explore freely for 5 minutes;

[0042] When the time is up, the telescopic unit operates to reset a new set of objects. The lifting unit then lifts the movable plate to the magnet on the cage to attract the iron parts. After that, the lifting unit lowers the movable plate to reset, completing the operation of removing the rat from the new object recognition box.

[0043] The alcohol atomization unit operates, and the first and second nozzle assemblies are activated simultaneously to disinfect and deodorize a group of new objects and the new object recognition box.

[0044] Phase Three:

[0045] The third phase can begin one hour after the second phase ends;

[0046] The floor replacement unit replaces the flooring of temporary rearing cages;

[0047] The lifting unit raises the movable plate until it is in contact with the lower surface of the floor of the temporary feeding cage. The electromagnet is energized to attract iron parts. Then the lifting unit lowers the movable plate to reset, completing the operation of moving the rat into the new object recognition box. The electromagnet is then de-energized.

[0048] The rotating mechanism operates, driving the turntable to switch to a new set of adjacent objects facing the movable door;

[0049] The telescopic unit operates, causing a group of new objects currently facing the movable door to push open the movable door and extend into the open space 5cm away from the inner wall of the new object recognition box;

[0050] Allow the rats to explore freely for 5 minutes;

[0051] The camera starts recording for 5 minutes and saves the recorded video.

[0052] When the time is up, the telescopic unit operates to reset the current set of new objects. The lifting unit then lifts the movable plate to the magnet on the cage to attract the iron parts. After that, the lifting unit lowers the movable plate to reset, completing the operation of removing the rat from the new object recognition box.

[0053] The alcohol atomization unit operates, and the first and second nozzle assemblies are activated simultaneously to disinfect and deodorize a group of new objects and the new object identification box.

[0054] The beneficial effects of this invention are as follows:

[0055] Phase 1:

[0056] Rats are placed in temporary enclosures and then secured to the mounting slots on the top panel. A lifting unit raises a movable plate until it aligns with the lower surface of the enclosure floor. An electromagnet is energized to attract iron components, and then the lifting unit lowers the plate back to its original position, completing the transfer of the rat into the new object recognition box. The electromagnet is then de-energized, and the rat is allowed to explore freely for 10 minutes. After the familiarization period, the lifting unit raises the movable plate again until it attracts iron components to the cage. The lifting unit then lowers the plate back to its original position, completing the removal of the rat from the new object recognition box. Simultaneously, the alcohol atomization unit activates, and the second nozzle assembly is turned on to disinfect and deodorize the new object recognition box.

[0057] Phase Two:

[0058] The floor replacement unit replaces the floor of the temporary feeding cage; the lifting unit raises the movable plate until it is flush with the lower surface of the temporary feeding cage floor, the electromagnet is energized to attract iron parts, and then the lifting unit lowers the movable plate to its original position, completing the transfer of the rat into the new object recognition box. The electromagnet is then de-energized. The telescopic unit operates, pushing a set of new objects through the movable door and extending them into the open space 5cm from the inner wall of the new object recognition box; the rats are allowed to explore freely for 5 minutes; after 5 minutes, the telescopic unit operates, resetting the set of new objects, and the lifting unit raises the movable plate to the magnet on the cage body to attract iron parts, and then the lifting unit lowers the movable plate to its original position, completing the transfer of the rat out of the new object recognition box; the alcohol atomization unit operates, with the first and second nozzle assemblies activating simultaneously to disinfect and deodorize the set of new objects and the new object recognition box.

[0059] Phase Three:

[0060] The third stage can begin one hour after the end of the second stage. The floor replacement unit replaces the floor of the temporary feeding cage. The lifting unit raises the movable plate until it is flush with the lower surface of the temporary feeding cage floor. An electromagnet is energized to attract iron parts, and then the lifting unit lowers the movable plate to its original position, completing the transfer of the rat into the new object recognition box. The electromagnet is then de-energized. The rotating mechanism operates, driving the turntable to switch to an adjacent set of new objects facing the activity door. The telescopic unit operates, causing the set of new objects currently facing the activity door to push open the door and extend into the open space 5cm from the inner wall of the new object recognition box. The rat is allowed to explore freely for 5 minutes. The camera begins recording for 5 minutes and saves the recorded video. After the time is up, the telescopic unit operates, causing the current set of new objects to return to its original position. The lifting unit raises the movable plate to the magnet on the cage body to attract iron parts, and then the lifting unit lowers the movable plate to its original position, completing the transfer of the rat out of the new object recognition box. The alcohol atomization unit operates, and the first and second nozzle assemblies are simultaneously activated to disinfect and deodorize the set of new objects and the new object recognition box.

[0061] By applying the methods of this application, the operation of moving rats, cleaning and disinfection, and switching to new objects during the experiment can be fully automated without manual operation, reducing human intervention and making the experimental results more accurate. At the same time, it avoids the burden of frequent manual operation and can greatly improve experimental efficiency. Attached Figure Description

[0062] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the present invention will be further described below in conjunction with the accompanying drawings and embodiments. The drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0063] Figure 1 This is a cross-sectional view of an object recognition device for detecting cognitive function in rats with vascular cognitive impairment, according to a preferred embodiment of the present invention.

[0064] Figure 2 This is a schematic diagram of the bottom structure of a temporary feeding cage for an object recognition device for detecting the cognitive function of rats with vascular cognitive impairment, according to a preferred embodiment of the present invention. Detailed Implementation

[0065] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, a clear and complete description will be provided below in conjunction with the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the protection scope of the present invention.

[0066] A preferred embodiment of the object recognition device for detecting cognitive function in rats with vascular cognitive impairment according to the present invention, such as... Figure 1 As shown, see also Figure 2 The system includes a mounting column 1, a base plate 2 fixed to the lower end of the mounting column 1, and a top plate 3 fixed to the upper end of the mounting column 1. A new object recognition box 4 is mounted on the upper surface of the base plate 2. A movable door 40 is located on the side of the new object recognition box 4 closest to the mounting column 1. A turntable 10 and a rotating mechanism 11 that drives the turntable 10 are mounted on the mounting column 1. Two telescopic units 12 are mounted on the turntable 10. A set of new objects 13 is located at the front end of each telescopic unit 12, and the two sets of new objects 13 contain only one common new object. A temporary feeding cage 5 and a fixing groove 30 for fixing the temporary feeding cage 5 are mounted on the top plate 3 (the cage can be fixed by clamping a fixing component, by interference fit with the cage, or by other existing methods; a limit block 520 can be installed on the cage 52). A through hole 31 is opened at the bottom of the fixing groove 30 for temporary feeding. The cage 5 has multiple iron pieces 51 arranged around the edge of the floor 50, and multiple magnets 53 corresponding to attract the iron pieces are arranged on the cage body 52. ​​The floor 50 can pass through the through hole 31 longitudinally. A camera 54 is detachably installed on the inner top of the cage body 52. ​​The top plate 3 is equipped with a floor replacement unit 6 for replacing the floor. The bottom of the new object recognition box 4 is hollowed out. The bottom plate 2 is equipped with a movable plate 20 corresponding to the new object recognition box 4 and a lifting unit 21 for driving the movable plate 20 to rise and fall. The movable plate 20 is equipped with an electromagnet 22 corresponding to attract the multiple iron pieces 51. The mounting column 1 is equipped with an alcohol atomizing unit 14 and a first nozzle assembly 15 and a second nozzle assembly 16 connected to the alcohol atomizing unit 14. The first nozzle assembly 15 is used to spray the new object, and the second nozzle assembly 16 is partially set on the movable plate 20 for spraying the inner wall of the new object recognition box 4.

[0067] Phase 1:

[0068] Rats are placed into temporary rearing cage 5, and then the temporary rearing cage 5 is placed into the fixing groove 30 on the top plate 3 to fix the cage body 52 (it can be fixed by clamping parts or by interference fit). The lifting unit 21 drives the movable plate 20 to rise to fit against the lower surface of the floor 50 of the temporary rearing cage 5. The electromagnet 22 is energized to attract the iron parts 51. Then the lifting unit 21 drives the movable plate 20 to fall back to its original position (relying on the stronger attraction of the electromagnet 22 to the iron parts 51, the floor 50 is lifted from the cage). (Tear it off from body 52) to complete the operation of moving the rat into the new object recognition box 4. The electromagnet 22 is de-energized, and the rat is allowed to explore freely for 10 minutes. After the familiarization time is up, the lifting unit 21 drives the movable plate 20 to be lifted to the magnet 53 on the cage body 52 to attract the iron part 51. Then the lifting unit 21 drives the movable plate 20 to descend and reset, completing the operation of moving the rat out of the new object recognition box 4. The alcohol atomization unit 14 is activated, and the second nozzle assembly 16 is turned on to disinfect and deodorize the inner wall of the new object recognition box 4.

[0069] Phase Two:

[0070] The floor replacement unit 6 replaces the floor 50 of the temporary feeding cage 5; the lifting unit 21 drives the movable plate 20 to rise to fit against the lower surface of the floor 50 of the temporary feeding cage 5, the electromagnet 22 is energized to attract the iron part 51, and then the lifting unit 21 drives the movable plate 20 to fall back to its original position, completing the operation of moving the rat into the new object recognition box 4, and the electromagnet 22 is de-energized; the telescopic unit 12 operates, pushing a set of new objects 13 open the movable door 40 and extending them into the open space 5cm away from the inner wall of the new object recognition box 4; the rat is allowed to explore freely for 5 minutes; after the time is up, the telescopic unit 12 operates to move the set of new objects 13 back to its original position, the lifting unit 21 drives the movable plate 20 to rise to the magnet 53 on the cage body 52 to attract the iron part 51, and then the lifting unit 21 drives the movable plate 20 to fall back to its original position, completing the operation of moving the rat out of the new object recognition box 4; the alcohol atomization unit 14 operates, and at the same time the first nozzle assembly 15 and the second nozzle assembly 16 are turned on to disinfect and deodorize the set of new objects 13 and the new object recognition box 4.

[0071] Phase Three:

[0072] The third stage can begin one hour after the end of the second stage; the floor replacement unit 6 replaces the floor 50 of the temporary feeding cage 5; the lifting unit 21 drives the movable plate 20 to be lifted to fit against the lower surface of the floor 50 of the temporary feeding cage 5, the electromagnet 22 is energized to attract the iron part 51, and then the lifting unit 21 drives the movable plate 20 to descend and reset, completing the operation of moving the rat into the new object recognition box 4, and the electromagnet 22 is de-energized; the rotating mechanism 11 operates, driving the turntable 10 to operate, switching to an adjacent set of new objects 13 (there is one new object that is different from the previous set of new objects, such as two squares before, now there is one square and one sphere) facing the movable door 40; the telescopic unit 12 operates, driving the current object facing the movable door 40 to move towards the movable door. A new set of objects 13 facing the rat pushes open the movable door 40 and extends into the open space 5cm away from the inner wall of the new object recognition box 4; the rat is allowed to explore freely for 5 minutes; the camera 54 starts recording for 5 minutes and saves the recorded video; after the time is up, the telescopic unit 12 operates to reset the current set of new objects 13, and the lifting unit 21 drives the movable plate 20 to be lifted to the magnet 53 on the cage body 52 to attract the iron part 51. Then the lifting unit 21 drives the movable plate 20 to descend and reset, completing the operation of removing the rat from the new object recognition box 4; the alcohol atomization unit 14 operates, and at the same time the first nozzle assembly 15 and the second nozzle assembly 16 are turned on to disinfect and deodorize the set of new objects 13 and the new object recognition box 4.

[0073] By applying the method of this application, the operation of moving rats, cleaning and disinfection, and switching to new objects during the experiment can be fully automated without manual operation, reducing human intervention and making the experimental results more accurate. At the same time, it avoids the burden of frequent manual operation and can greatly improve experimental efficiency.

[0074] Understandably, the units composed of the above modules can be set up in multiple groups side by side (the bottom plate and the top plate can share the same one) to realize synchronous experiments at multiple experimental stations and further improve experimental efficiency.

[0075] Preferably, the lower end surface of the cage body 52 of the temporary feeding cage 5 is provided with a movable groove 521, and both ends of the movable groove 521 are connected to the outer surface of the cage body 52; the floor 50 is movably disposed in the movable groove 521; multiple magnets 53 are embedded in the inner wall of the movable groove 521; multiple iron pieces 51 are longitudinally inserted through both ends of the floor 50; the floor replacement unit 6 includes a stacking cylinder 60 for stacking floorboards disposed on the top plate 3, and a replacement groove 32 connected to the fixed groove 30 is provided on the top plate 3, and the stacking cylinder 60 is mounted on the replacement groove 32; the floor replacement unit 6 also includes a first cylinder for pushing the bottommost floorboard of the stack into the movable groove 521 to replace the current floorboard. 61; When replacing the floor, the first cylinder 61 pushes out the bottommost floor stacked in the stacking cylinder 60. This floor will squeeze the floor 50 currently installed on the cage 52 and slowly squeeze the floor 50 out (this process needs to be slowed down appropriately to avoid startling the rats). After the replacement is completed, a brand new clean floor is obtained. By replacing the floor, the process of disinfecting the floor is omitted. Preferably, the top plate 3 is provided with a receiving box 33 to receive the replaced floor. The dirty floor will be collected by the receiving box. After a certain number are collected, they will be cleaned, disinfected and dried and then put back into the stacking cylinder 60 for reuse.

[0076] Preferably, the telescopic unit 12 includes a second cylinder 120, and the movable end of the second cylinder 120 is provided with a horizontal plate 121 for installing a set of new objects; the inner wall of the new object identification box 4 is provided with a slot that cooperates with the movable door 40, and the upper end of the movable door 40 is rotatably connected to the inner top of the slot through a rotating shaft 41, and a torsion spring is sleeved on the rotating shaft; the structure is simple and the cost is low; preferably, the lower end of the movable door 40 is also provided with a clearance groove to avoid the rod of the second cylinder 120.

[0077] Preferably, the lifting unit 21 includes a third cylinder, and the movable end of the third cylinder is fixed with a movable plate 20; the second nozzle assembly 16 includes a multi-port pipe 160, the main pipe of the multi-port pipe 160 is provided with a solenoid valve 161 and is connected to the mist outlet of the alcohol atomizing unit 14, and the sub-pipes of the multi-port pipe 160 are arranged in a ring on the bottom surface of the movable plate 20; the electromagnet 22 is fixed on the surface of the movable plate 20; the structure is simple, and the second nozzle assembly 16 can spray alcohol mist on the inner wall of the new object recognition box 4 relatively evenly to remove odors; preferably, the outer surface of the movable plate 20 can also be provided with a brush to wipe the small alcohol droplets on the inner wall of the cage.

[0078] A method for detecting object recognition in rats with vascular cognitive impairment, using the object recognition device described above, comprising the steps of:

[0079] Phase 1:

[0080] Rats were placed in temporary feeding cages, and then the temporary feeding cages were placed in the fixing slots on the top plate to fix the cages.

[0081] The lifting unit raises the movable plate until it is in contact with the lower surface of the floor of the temporary feeding cage. The electromagnet is energized to attract iron parts. Then the lifting unit lowers the movable plate to reset, completing the operation of moving the rat into the new object recognition box. The electromagnet is de-energized, and the rat is allowed to explore freely after 10 minutes.

[0082] Once the familiarization time is up, the lifting unit drives the movable plate to rise to the magnet on the cage body to attract the iron parts. Then the lifting unit drives the movable plate to fall back to its original position, completing the operation of removing the rat from the new object recognition box.

[0083] The alcohol atomization unit operates, and the second nozzle assembly is activated to disinfect and deodorize the new object recognition box.

[0084] Phase Two:

[0085] The floor replacement unit replaces the flooring of temporary rearing cages;

[0086] The lifting unit raises the movable plate until it is in contact with the lower surface of the floor of the temporary feeding cage. The electromagnet is energized to attract iron parts. Then the lifting unit lowers the movable plate to reset, completing the operation of moving the rat into the new object recognition box. The electromagnet is then de-energized.

[0087] The telescopic unit operates, pushing a group of new objects through the movable door and extending them into the open space 5cm away from the inner wall of the new object recognition box;

[0088] Allow the rats to explore freely for 5 minutes;

[0089] When the time is up, the telescopic unit operates to reset a new set of objects. The lifting unit then lifts the movable plate to the magnet on the cage to attract the iron parts. After that, the lifting unit lowers the movable plate to reset, completing the operation of removing the rat from the new object recognition box.

[0090] The alcohol atomization unit operates, and the first and second nozzle assemblies are activated simultaneously to disinfect and deodorize a group of new objects and the new object recognition box.

[0091] Phase Three:

[0092] The third phase can begin one hour after the second phase ends;

[0093] The floor replacement unit replaces the flooring of temporary rearing cages;

[0094] The lifting unit raises the movable plate until it is in contact with the lower surface of the floor of the temporary feeding cage. The electromagnet is energized to attract iron parts. Then the lifting unit lowers the movable plate to reset, completing the operation of moving the rat into the new object recognition box. The electromagnet is then de-energized.

[0095] The rotating mechanism operates, driving the turntable to switch to a new set of adjacent objects facing the movable door;

[0096] The telescopic unit operates, causing a group of new objects currently facing the movable door to push open the movable door and extend into the open space 5cm away from the inner wall of the new object recognition box;

[0097] Allow the rats to explore freely for 5 minutes;

[0098] The camera starts recording for 5 minutes and saves the recorded video.

[0099] When the time is up, the telescopic unit operates to reset the current set of new objects. The lifting unit then lifts the movable plate to the magnet on the cage to attract the iron parts. After that, the lifting unit lowers the movable plate to reset, completing the operation of removing the rat from the new object recognition box.

[0100] The alcohol atomization unit operates, and the first and second nozzle assemblies are activated simultaneously to disinfect and deodorize a group of new objects and the new object recognition box.

[0101] By applying the methods of this application, the operation of moving rats, cleaning and disinfection, and switching to new objects during the experiment can be fully automated without manual operation, reducing human intervention and making the experimental results more accurate. At the same time, it avoids the burden of frequent manual operation and can greatly improve experimental efficiency.

[0102] It should be understood that those skilled in the art can make improvements or modifications based on the above description, and all such improvements and modifications should fall within the protection scope of the appended claims.

Claims

1. An object recognition device for detecting cognitive function in rats with vascular cognitive impairment, characterized in that, The system includes a mounting column, a base plate fixed to the lower end of the mounting column, and a top plate fixed to the upper end of the mounting column. A new object recognition box is mounted on the upper surface of the base plate, and a movable door is located on the side of the new object recognition box closest to the mounting column. A turntable and a rotating mechanism for rotating the turntable are mounted on the mounting column. Two telescopic units are mounted on the turntable, and a set of new objects is positioned at the front end of each telescopic unit. The two sets of new objects share only one common new object. A temporary feeding cage and a fixing groove for fixing the temporary feeding cage are mounted on the top plate. A through hole is opened at the bottom of the fixing groove. Multiple iron pieces are arranged around the edge of the floor of the temporary feeding cage, and multiple corresponding suction devices are installed on the cage body. The cage contains magnets for iron parts; the floor can pass longitudinally through the through hole; a camera is detachably installed on the inner top of the cage; a floor replacement unit is provided on the top plate for replacing the floor; the bottom of the new object recognition box is hollowed out; a movable plate corresponding to the new object recognition box and a lifting unit for raising and lowering the movable plate are provided on the bottom plate; electromagnets corresponding to attracting multiple iron parts are provided on the movable plate; an alcohol atomizing unit and a first nozzle assembly and a second nozzle assembly connected to the alcohol atomizing unit are provided on the mounting column; the first nozzle assembly is used to spray the new object; the second nozzle assembly is partially installed on the movable plate and is used to spray the inner wall of the new object recognition box.

2. The object recognition device for detecting cognitive function in rats with vascular cognitive impairment according to claim 1, characterized in that, The temporary feeding cage has a movable groove on the lower end surface of the cage body, and both ends of the movable groove are connected to the outer surface of the cage body; the floor is movably disposed in the movable groove; a plurality of magnets are embedded in the inner wall of the movable groove; and a plurality of iron pieces are longitudinally inserted through both ends of the floor.

3. The object recognition device for detecting cognitive function in rats with vascular cognitive impairment according to claim 2, characterized in that, The floor replacement unit includes a stacking cylinder for stacking floorboards disposed on the top plate. The top plate is provided with a replacement slot communicating with the fixing slot, and the stacking cylinder is mounted on the replacement slot. The floor replacement unit also includes a first cylinder that pushes the bottommost floor of the stack into a movable slot to replace the current floor.

4. The object recognition device for detecting cognitive function in rats with vascular cognitive impairment according to claim 3, characterized in that, The top plate is equipped with a receiving box for receiving the replaced floorboards.

5. The object recognition device for detecting cognitive function in rats with vascular cognitive impairment according to claim 1, characterized in that, The telescopic unit includes a second cylinder, the movable end of which is provided with a horizontal plate for installing a set of new objects; the inner wall of the new object identification box is provided with a slot that cooperates with the movable door, the upper end of the movable door is rotatably connected to the inner top of the slot through a rotating shaft, and a torsion spring is sleeved on the rotating shaft.

6. The object recognition device for detecting cognitive function in rats with vascular cognitive impairment according to claim 1, characterized in that, The lifting unit includes a third cylinder, and the movable plate is fixed to the movable end of the third cylinder.

7. The object recognition device for detecting cognitive function in rats with vascular cognitive impairment according to claim 6, characterized in that, The second nozzle assembly includes a multi-port pipe, the main pipe of which is equipped with a solenoid valve and communicates with the mist outlet of the alcohol atomizing unit. The sub-pipes of the multi-port pipe are arranged in a ring on the bottom surface of the movable plate. The electromagnet is fixed on the surface of the movable plate.

8. A method for detecting the cognitive function of rats with vascular cognitive impairment, using the object recognition device for detecting the cognitive function of rats with vascular cognitive impairment as described in any one of claims 1-7, characterized in that, The method includes the following steps: Phase 1: Rats were placed in temporary feeding cages, and then the temporary feeding cages were placed in the fixing slots on the top plate to fix the cages. The lifting unit raises the movable plate until it is in contact with the lower surface of the floor of the temporary feeding cage. The electromagnet is energized to attract iron parts. Then the lifting unit lowers the movable plate to reset, completing the operation of moving the rat into the new object recognition box. The electromagnet is de-energized, and the rat is allowed to explore freely after 10 minutes. Once the familiarization time is up, the lifting unit drives the movable plate to rise to the magnet on the cage body to attract the iron parts. Then the lifting unit drives the movable plate to fall back to its original position, completing the operation of removing the rat from the new object recognition box. The alcohol atomization unit operates, and the second nozzle assembly is activated to disinfect and deodorize the new object recognition box. Phase Two: The floor replacement unit replaces the flooring of temporary rearing cages; The lifting unit raises the movable plate until it is in contact with the lower surface of the floor of the temporary feeding cage. The electromagnet is energized to attract iron parts. Then the lifting unit lowers the movable plate to reset, completing the operation of moving the rat into the new object recognition box. The electromagnet is then de-energized. The telescopic unit operates, pushing a group of new objects through the movable door and extending them into the open space 5cm away from the inner wall of the new object recognition box; Allow the rats to explore freely for 5 minutes; When the time is up, the telescopic unit operates to reset a new set of objects. The lifting unit then lifts the movable plate to the magnet on the cage to attract the iron parts. After that, the lifting unit lowers the movable plate to reset, completing the operation of removing the rat from the new object recognition box. The alcohol atomization unit operates, and the first and second nozzle assemblies are activated simultaneously to disinfect and deodorize a group of new objects and the new object recognition box. Phase Three: The third phase can begin one hour after the second phase ends; The floor replacement unit replaces the flooring of temporary rearing cages; The lifting unit raises the movable plate until it is in contact with the lower surface of the floor of the temporary feeding cage. The electromagnet is energized to attract iron parts. Then the lifting unit lowers the movable plate to reset, completing the operation of moving the rat into the new object recognition box. The electromagnet is then de-energized. The rotating mechanism operates, driving the turntable to switch to a new set of adjacent objects facing the movable door; The telescopic unit operates, causing a group of new objects currently facing the movable door to push open the movable door and extend into the open space 5cm away from the inner wall of the new object recognition box; Allow the rats to explore freely for 5 minutes; The camera starts recording for 5 minutes and saves the recorded video. When the time is up, the telescopic unit operates to reset the current set of new objects. The lifting unit then lifts the movable plate to the magnet on the cage to attract the iron parts. After that, the lifting unit lowers the movable plate to reset, completing the operation of removing the rat from the new object recognition box. The alcohol atomization unit operates, and the first and second nozzle assemblies are activated simultaneously to disinfect and deodorize a group of new objects and the new object identification box.

Images