一种大熊猫主食竹叶际微生物获得方法

By combining physical treatment with vortexing and ultrasound, and multi-step centrifugation purification, along with a culture medium containing bamboo leaf extract and plant hormone analogs, the problem of incomplete acquisition of microbial communities from the bamboo leaf, the staple food of giant pandas, was solved. This enabled the complete analysis of the microbial community and the efficient culture and identification of functional bacteria.

CN120905072BActive Publication Date: 2026-07-17CHINA WEST NORMAL UNIVERSITY

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA WEST NORMAL UNIVERSITY
Filing Date
2025-08-07
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing technologies are insufficient to effectively obtain the microbial community of bamboo, the staple food of giant pandas, resulting in distorted analysis of microbial composition, a low proportion of culturable microorganisms, and a lack of multi-dimensional analysis systems.

Method used

Microbial enrichment was achieved through a physical treatment method combining vortexing and ultrasound, combined with multi-step centrifugation purification. Bamboo leaf extract and plant hormone analogs were added to the culture medium to establish a culture mode combining solid plate and liquid shaking, thus constructing a technical system of "sampling-enrichment-culture-identification" and "sampling-enrichment-identification".

Benefits of technology

This approach enables the complete analysis of phyllosphere microbial communities and the targeted discovery of functional bacteria, improving the success rate of microbial culture and the coverage of identification, and ensuring the diversity and accuracy of identification results.

✦ Generated by Eureka AI based on patent content.

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Abstract

本发明公开了一种大熊猫主食竹叶际微生物获得方法,属于微生物鉴定技术领域;包括步骤:S1.采样;S2.微生物富集:将竹叶样品放入无菌塑封袋中,加入缓冲液对样品进行涡旋和超声处理。洗涤结束取出竹叶并将无菌塑封袋内的液体进行离心,弃上清收集菌体,其后加入缓冲液重悬菌体,后进行二次离心,最后去上清,再次重悬并离心,收集菌泥,‑80℃冷冻保存备用。S3.微生物培养:取步骤S2中的菌泥采用固体培养平板培养和液体振荡培养相结合的方式进行培养;固体培养基和液体培养基中均加有竹叶浸出液、植物激素类似物等;S4.微生物鉴定。本发明实现了对竹叶际微生物从原始样本到培养菌群的全面分析,确保鉴定结果覆盖群落多样性。
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