Engineering bacterium for efficiently producing adenine as well as construction method and application of engineering bacterium

Abstract

The invention belongs to the technical field of genetic engineering, and particularly relates to an engineering bacterium for efficiently producing adenine as well as a construction method and application of the engineering bacterium. According to the invention, the corynebacterium glutamicum ATCC 13032 is subjected to multi-target gene modification, including the knockout of PknG, purR, pta and apt, the knockout of PknG, purR, pta and apt are respectively replaced with glnA, PRPP, zwf and ppk, and the purA and purB are over-expressed by virtue of a recombinant plasmid pXMJ19-purA-purB, so that the high-yield adenine engineering bacterium is successfully constructed. The engineering bacterium is combined with a specific whole-cell catalysis technology, so that the yield of adenine reaches 6.7 g / L which is obviously higher than that in the prior art, the biosynthesis efficiency of adenine is greatly improved, a feasible technical scheme is provided for large-scale biological preparation of adenine, and the engineering bacterium has important industrial application value.

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