Method for improving dehydrogenase activity based on nano bubble water

Abstract

The invention relates to the technical field of application of water treatment, biology, health and the like, in particular to a method for improving dehydrogenase activity based on nano bubble water. The method comprises the following steps: (1) preparing nano bubble water in water through a pressurization and decompression method; and (2) respectively adding ethanol dehydrogenase and acetaldehyde dehydrogenase into the nano bubble water to form an enzyme-nano bubble composite system. The nano bubble water prepared by the invention has ultrahigh stability, high specific surface area, high interface energy and unique interface characteristics, and can promote enzyme conformation stability, substrate recognition and electron transfer under the condition of not introducing any chemical modifier, thereby synergistically enhancing the catalytic activity of ethanol dehydrogenase and acetaldehyde dehydrogenase. The method is simple and convenient to operate and mild in condition, and the obtained system is clear, transparent and good in stability, has the advantages of being green, safe, free of additives, high in adaptability and the like, and is suitable for various biological enzyme catalysis application scenes.

Images