Headspace-solid phase microextraction-based nondestructive detection method for poria cocos doped with cassava

Abstract

The invention relates to a nondestructive testing method for poria cocos doped with cassava based on headspace-solid phase microextraction. The method comprises the following steps: (1) pre-treating a sample; (2) micro-extraction adsorption: adopting a DVB / CAR / PDMS three-component composite solid-phase micro-extraction needle aged at 250 DEG C, and adsorbing for 40 + / -2 minutes at the constant temperature of 25 + / -1 DEG C; (3) thermal desorption analysis: inserting the extraction needle after adsorption into a gas chromatograph-mass spectrometer equipped with a cold trap focusing module, carrying out desorption at a 260 DEG C sample inlet for 300 seconds, and improving peak shape sharpness through secondary focusing; (4) setting chromatographic separation conditions; (5) determining mass spectrum detection conditions; and (6) carrying out combined judgment on the characteristic markers: when anethole and decanol are detected at the same time, judging that the cassava is doped in the poria cocos sample. The method can be used for non-destructive identification.

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