Ganoderma lucidum beta-glucan high-throughput rapid detection method

Abstract

The invention discloses a ganoderma lucidum beta-glucan high-flux rapid detection method, and belongs to the technical field of polysaccharide detection.The ganoderma lucidum beta-glucan high-flux rapid detection method is characterized in that graphene oxide serves as a carrier and a fluorescence quencher, a fluorescence-modified nucleic acid aptamer and the specific binding capacity of the aptamer to beta-1, 3-1, 6-glucan are utilized, and the specific binding capacity of the aptamer to beta-1, 3-1, 6-glucan is improved; according to the method, beta-1, 3-glucan and beta-1, 6-glucan in the ganoderma lucidum sample can be rapidly detected, and the detection method is simple in step, easy and convenient to operate, high in detection rate, good in detection stability and easy to popularize and use.

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