A device for simulating and identifying the salt-alkali tolerance gradient of wild soybean throughout its entire growth period

Abstract

This invention discloses a salt-alkali tolerance gradient simulation and identification device for wild soybean throughout its entire growth period, relating to the field of crop breeding technology. It includes: a planting simulation component for adaptively adjusting salt and alkali levels according to the growth characteristics of wild soybean; a cover that covers the planting simulation component to form a sealed space together, the cover also having an exhaust pipe; and a first gas tank connected to the sealed space, pre-filled with carbon dioxide gas. This device can monitor the amount of organic acid secreted by the roots in real time and dynamically match the salt-alkali tolerance of wild soybean at its current growth stage, providing high data precision.

Description

Technical Field

[0001] This invention relates to the field of crop breeding technology, specifically to a device for simulating and identifying the salt and alkali tolerance gradient of wild soybean throughout its entire growth period. Background Technology

[0002] Wild soybean, as an important resource of salt-alkali tolerance genes, requires effective identification of its salt-alkali tolerance throughout its entire growth cycle. This is crucial for breeding highly salt-alkali tolerant soybean varieties and alleviating the challenges of agricultural utilization of saline-alkali land. However, existing salt-alkali tolerance identification devices have significant limitations in terms of technology and cannot meet the needs of dynamic research throughout the entire growth cycle.

[0003] Currently, most mainstream salt-alkali tolerance assessment devices employ a "fixed salt-alkali gradient" testing method. This involves pre-setting several discrete salt-alkali gradients (low, medium, and high) and roughly determining the range of salt-alkali tolerance by comparing plant survival rates or growth indicators under different gradients. The core drawback of this method is that the regulation of salt-alkali is static and cannot be dynamically adjusted according to the plant's real-time physiological state. This easily leads to problems such as excessively high salt-alkali levels causing excessive stress or insufficiently low salt-alkali levels failing to fully activate stress tolerance. It can only define a general range of tolerance or intolerance, resulting in coarse and unreliable data, making it difficult to support in-depth research on stress tolerance mechanisms throughout the entire growth cycle.

[0004] In addition, the natural growing environment of wild soybeans is complex. Their salt and alkali tolerance is not only affected by salinity, but also closely related to factors such as temperature, humidity and gas environment. However, existing technologies usually only focus on the single factor of salinity.

[0005] The existing devices also have shortcomings in terms of structural design and operation. Using fixed-position spraying or soil replenishment can easily lead to uneven local supply due to differences in the position of the spray nozzles. The planting carriers are mostly fixed by simple clips or direct placement, which can easily be displaced by the impact of spraying, plant root growth, or equipment vibration, affecting the uniformity of supply or even interrupting the experiment.

[0006] Therefore, it is necessary to provide a device for simulating and identifying the salt and alkali gradient tolerance of wild soybean throughout its entire growth period in order to solve the above problems. Summary of the Invention

[0007] To address the above problems, the present invention provides the following technical solution: a wild soybean whole-growth-cycle salt-alkali gradient simulation identification device, comprising:

[0008] A planting simulation component is used to adaptively adjust salinity based on the growth characteristics of wild soybeans.

[0009] A cover is provided on the planting simulation component to form a sealed space together with the planting simulation component; the cover is also provided with an exhaust pipe.

[0010] A first gas cylinder, which is connected to the sealed space, is pre-filled with carbon dioxide gas.

[0011] Furthermore, preferably, the planting simulation component includes:

[0012] A base plate, together with the cover, forms the sealed space, and a drain pipe is provided on the base plate;

[0013] A toothed ring, which is rotatably mounted on the base plate;

[0014] Multiple circumferentially distributed rotary drive components are embedded in the base plate, and each rotary drive component has a rotary drive end.

[0015] Multiple support components are correspondingly installed on the rotary drive end, and the support components are used as carriers for wild soybean cultivation;

[0016] A spraying and supply assembly is also fixed on the toothed ring, which is used to assist in supplying water or soil to the bearing assembly.

[0017] Furthermore, preferably, the spraying and supply assembly includes:

[0018] Side seat, which is fixed to the toothed ring;

[0019] A vertical drive assembly is disposed on the side seat, and the vertical drive assembly has a vertical drive end;

[0020] The sprayer and soil supply chamber are installed at the vertical drive end.

[0021] Furthermore, preferably, the rotary drive assembly includes:

[0022] The outer ring is embedded in the base plate;

[0023] Multiple arc seats are distributed at circumferential intervals, and the arc seats are installed on the inner wall of the outer ring by springs;

[0024] A drive wheel is rotatably mounted on the arc seat.

[0025] Furthermore, preferably, the carrier component includes:

[0026] The carrying chamber has a partition fixed inside to divide it into an upper chamber and a lower chamber, and the partition has a through hole in the middle.

[0027] A net cylinder with a plate fixed in the middle, the top of which is used to support soil and wild soybean seeds;

[0028] A measuring rod, which penetrates the cylinder plate and passes through the through hole, is used to measure the organic acid secretion of the wild soybean seeds and the salinity of the liquid in the lower chamber;

[0029] Multiple liquid supply pipes are distributed in a circular pattern, the liquid supply pipes penetrate the partition, the bottom of the liquid supply pipe has an inlet and the top has an outlet, and a seepage ring is installed at the outlet.

[0030] Furthermore, as a preferred embodiment, a first liquid supply chamber and a second liquid supply chamber are provided below the bearing chamber for supplying liquid to the lower chamber, and the salinity of the liquid in the lower chamber is adjusted by regulating the supply volume of the two chambers.

[0031] Furthermore, as a preferred embodiment, the upper compartment is provided with a second drain valve, and the lower compartment is provided with a first drain valve.

[0032] Furthermore, preferably, the bottom of the mesh cylinder has a folded edge;

[0033] A hinged seat is detachably connected to the partition, and a locking claw is hinged to the hinged seat. One end of the locking claw abuts against the folded edge, and the other end abuts against the partition.

[0034] Furthermore, as a preferred embodiment, a heating plate is fixed below the partition.

[0035] Furthermore, as a preferred embodiment, a temperature and humidity sensor is provided on the inner wall of the housing;

[0036] A second gas cylinder for supplying dry gas to the sealed space is also provided on one side of the cover.

[0037] Compared with the prior art, the present invention provides a device for simulating and identifying the salt-alkali gradient tolerance of wild soybean throughout its entire growth period, which has the following beneficial effects:

[0038] In this invention, the amount of organic acid secreted by the roots is monitored in real time by measuring rod. Combined with the liquid supply ratio adjustment technology of the first and second liquid supply chambers, the salt and alkali tolerance of wild soybeans at the current growth stage can be dynamically matched. The data precision is far superior to traditional methods, providing a more reliable basis for studying the salt and alkali tolerance mechanism of wild soybeans throughout the entire growth period.

[0039] In this invention, the temperature and humidity within a sealed space are precisely controlled through the linkage of a temperature and humidity sensor, a heating plate, and a second gas tank, simulating the temperature and humidity changes during the natural growth period. Simultaneously, the first gas tank is pre-filled with carbon dioxide gas, which can inhibit microbial activity or simulate a specific gaseous environment. This synergistic simulation of multiple factors (salinity, temperature, humidity, and gas) more closely resembles the natural growth environment of wild soybeans, significantly improving the authenticity and applicability of the identification results.

[0040] In this invention, the sprayer and soil supply chamber rotate with the toothed ring, covering all the supporting components, realizing "mobile" uniform spraying and soil replenishment, while the supporting components can effectively carry wild soybean seeds and provide them with a real planting environment. Attached Figure Description

[0041] Figure 1 A schematic diagram of a planar structure for a wild soybean salt-alkali gradient simulation and identification device covering the entire growth period;

[0042] Figure 2 A three-dimensional schematic diagram of the planting simulation components and the spraying and supply components in a wild soybean full-growth-cycle salt-alkali gradient simulation identification device;

[0043] Figure 3 A three-dimensional schematic diagram of the spraying and supply components in a salt-alkali gradient simulation and identification device for the entire growth period of wild soybean;

[0044] Figure 4 A three-dimensional schematic diagram of the rotating drive component in a salt-alkali gradient simulation identification device for the entire growth period of wild soybean;

[0045] Figure 5 A three-dimensional schematic diagram of the supporting components in a salt-alkali gradient simulation and identification device for the entire growth period of wild soybean;

[0046] Figure 6 This is a schematic diagram of the planar structure of the supporting component in a salt-alkali gradient simulation and identification device for the entire growth period of wild soybean;

[0047] In the diagram: 1. First gas tank; 2. Second gas tank; 3. Planting simulation component; 4. Sewage pipe; 5. Cover; 6. Spraying and supply component; 7. Exhaust pipe; 31. Base plate; 32. Gear ring; 33. Gear; 34. First motor; 35. Rotary drive component; 36. Bearing component; 351. Outer ring; 352. Spring; 353. Arc seat; 354. Drive wheel; 361. Bearing chamber; 362. Net cylinder; 363. Locking claw 364. Hinge seat; 365. First liquid supply chamber; 366. Second liquid supply chamber; 367. Partition plate; 368. Heating plate; 369. First drain valve; 3610. Second drain valve; 3611. Cylindrical plate; 3612. Measuring rod; 3613. Liquid supply pipe; 3614. Seepage ring; 61. Side seat; 62. Guide rod; 63. Moving seat; 64. Lead screw; 65. Second motor; 66. Sprayer; 67. Soil supply chamber. Detailed Implementation

[0048] The terms "first," "second," etc., used in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such terms are interchangeable where appropriate; this is merely a way of distinguishing objects with the same attributes in the embodiments of this application. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion, so that a process, method, system, product, or apparatus that comprises a series of elements is not necessarily limited to those elements, but may include other elements not explicitly listed or inherent to those processes, methods, products, or apparatuses.

[0049] Example: Please refer to Figures 1-6 In this embodiment of the invention, a device for simulating and identifying the salt-alkali gradient tolerance of wild soybean throughout its entire growth period is provided, comprising:

[0050] Planting simulation component 3 is used to adaptively adjust salinity based on the growth characteristics of wild soybeans;

[0051] The cover 5 is placed on the planting simulation component 3 to form a sealed space together with the planting simulation component 3. The cover 5 is also provided with an exhaust pipe 7.

[0052] A first gas cylinder 1 is connected to the sealed space, and the first gas cylinder 1 is pre-filled with carbon dioxide gas.

[0053] This device uses a planting simulation component 3 to dynamically monitor and adaptively regulate the salt and alkali tolerance of wild soybeans throughout their entire growth cycle. Specifically, the casing 5 and the planting simulation component 3 together form a sealed space. The first gas tank 1 pre-fills this space with carbon dioxide gas to simulate a specific gas environment or inhibit microbial activity. The exhaust pipe 7 can regulate the gas pressure within the sealed space or expel excess gas, creating a relatively controllable simulated environment.

[0054] The planting simulation component 3 can monitor the amount of organic acids secreted by the roots in real time (organic acid secretion is an important mechanism for plants to cope with salt and alkali stress, and the amount of secretion can reflect the current salt and alkali tolerance status). Based on the monitored amount of organic acid secretion, the planting simulation component 3 dynamically adjusts the soil salinity to ensure that the salinity always matches the tolerance capacity of wild soybean at its current growth stage, avoiding the problems of excessive stress (too high salinity leading to plant death) or insufficient activation of stress tolerance (too low salinity making it impossible to measure the true tolerance limit) that may occur under the traditional "fixed gradient" method.

[0055] In other words, traditional fixed-gradient methods require setting up multiple control groups with different salinity levels (such as low, medium, and high gradients) and roughly judging the tolerance range by comparing plant survival rates, which is inefficient and produces coarse data. In contrast, this device dynamically adjusts salinity by monitoring plant feedback in real time, which can accurately measure the tolerance performance at each growth stage (such as germination, seedling, and flowering stages), providing more refined data and requiring a smaller sample size.

[0056] In this embodiment, the planting simulation component 3 includes:

[0057] The base plate 31, together with the cover 5, forms the sealed space, and a drain pipe 4 is provided on the base plate 31;

[0058] A toothed ring 32 is rotatably mounted on the base plate 31;

[0059] Multiple circumferentially distributed rotary drive components 35 are embedded in the base plate 31, and each rotary drive component 35 has a rotary drive end.

[0060] Multiple support components 36 are correspondingly installed on the rotary drive end, and the support components 36 are used as carriers for wild soybean cultivation;

[0061] A spraying and supplying component 6 is also fixed on the toothed ring 32. The spraying and supplying component 6 is used to assist in supplying water or soil to the bearing component 36.

[0062] The base plate 31 is equipped with a first motor 34, which drives a gear 33 to rotate. The gear 33 meshes with the gear ring 32. Therefore, the first motor 34 drives the gear ring 32 to rotate through the gear 33, and the gear ring 32 drives the spray and supply assembly 6 fixed thereon to rotate synchronously, realizing "rotational supply".

[0063] In this embodiment, the spraying and supply assembly 6 includes:

[0064] Side seat 61, which is fixed to the toothed ring 32;

[0065] A vertical drive assembly is disposed on the side seat 61, and the vertical drive assembly has a vertical drive end;

[0066] The sprayer 66 and the soil supply chamber 67 are installed at the vertical drive end.

[0067] Among them, the sprinkler 66 can be selected to spray at fixed times and in fixed quantities to maintain soil moisture.

[0068] The soil supply chamber 67 stores a small amount of spare soil (such as humus or standard test soil). When the soil in the net cylinder 362 is lost due to water erosion or plant root growth, it is rotated above the target bearing component 36 to reduce the height and release the soil to replenish the net cylinder 362, thus maintaining the stability of the planting carrier.

[0069] The vertical drive component includes:

[0070] At least two guide rods 62, the guide rods 62 being vertically fixed to the side seat 61;

[0071] The lead screw 64 is rotatably mounted on the side seat 61 and is driven by the second motor 65;

[0072] The movable seat 63 serves as the vertical drive end of the vertical drive assembly. The movable seat 63 is slidably disposed on the guide rod 62 and is connected to the lead screw 64 for transmission.

[0073] In this embodiment, the rotation drive assembly 35 includes:

[0074] The outer ring 351 is embedded in the base plate 31;

[0075] Multiple arc seats 353 are distributed in a circular interval, and the arc seats 353 are installed on the inner wall of the outer ring 351 by springs 352;

[0076] A drive wheel 354 is rotatably mounted on the arc seat 353.

[0077] When the rotary drive assembly 35 is working, the drive wheel 354 is driven to rotate by external power (such as a motor), and at the same time, the elasticity of the spring 352 causes the arc seat 353 to move towards the center (towards the bearing assembly 36), causing the drive wheel 354 to press tightly against the outer wall of the bearing assembly 36, forming an "elastic clamping".

[0078] The contact surface between the drive wheel 354 and the bearing component 36 generates friction, which pushes the bearing component 36 to rotate around its own axis. In this way, when in conjunction with the spraying and supply component 6, more comprehensive and uniform spraying and soil supply can be achieved.

[0079] In this embodiment, the carrier component 36 includes:

[0080] The carrying chamber 361 has a partition 367 fixed inside it, which is used to divide the carrying chamber 361 into an upper chamber and a lower chamber. The partition 367 has a through hole in the middle.

[0081] The net cylinder 362 has a cylinder plate 3611 fixed in its middle, and the top of the cylinder plate 3611 is used to support soil and wild soybean seeds;

[0082] Measuring rod 3612, which penetrates the cylinder plate 3611 and passes through the through hole, is used to measure the organic acid secretion of the wild soybean seeds and the salinity of the liquid in the lower chamber;

[0083] Multiple liquid supply pipes 3613 are distributed in a circular pattern, the liquid supply pipes 3613 penetrate the partition 367, the bottom of the liquid supply pipes 3613 has an inlet and the top has an outlet, and a seepage ring 3614 is installed at the outlet.

[0084] In other words, the bearing chamber 361 is divided into an upper chamber and a lower chamber by the partition 367. The mesh cylinder 362 is located in the upper chamber, and its cylinder plate 3611 supports soil and wild soybean seeds. The upper chamber is also filled with soil to simulate the natural soil environment. The measuring rod 3612 passes through the through hole of the cylinder plate 3611 and the partition 367, and can directly contact the wild soybean root system to monitor the amount of organic acid secreted by the root system in real time. At the same time, the bottom of the measuring rod 3612 extends to the lower chamber to monitor the salinity of the liquid. The seepage rings 3614 are distributed circumferentially at the top of the liquid supply pipe 3613. Through seepage, the liquid is evenly distributed to the soil around the mesh cylinder 362, avoiding excessively high or low local salinity concentrations and simulating the uniform distribution of salinity in natural soil.

[0085] It's important to explain that traditional fixed-gradient methods require setting up multiple experimental groups with different salinity levels (such as low, medium, and high gradients), and roughly determining the tolerance range by comparing plant survival rates. This is inefficient and produces coarse data. In contrast, this device dynamically adjusts salinity by monitoring plant feedback in real time, allowing for precise measurement of tolerance performance at each growth stage (such as germination, seedling, and flowering), resulting in more refined data.

[0086] In this embodiment, the upper and lower compartment separation structure isolates the soil from the liquid, preventing the liquid from directly soaking the seeds. At the same time, the seepage ring 3614 simulates the natural infiltration process of salt and alkali in the soil, which is closer to the saline-alkali environment in which wild soybeans grow naturally.

[0087] Among them, the seepage ring 3614 can be a "porous ceramic seepage ring", in which the ceramic micropores (pore diameter 0.1-1mm) allow the liquid to seep out slowly.

[0088] The measuring rod 3612 has an integrated electrochemical sensor at the top for measuring the organic acid secretions of the wild soybean seeds, and an integrated salinity sensor at the bottom for measuring the salinity of the liquid in the lower chamber.

[0089] In this embodiment, a first supply chamber 365 and a second supply chamber 366 are provided below the carrying chamber 361 for supplying liquid to the lower chamber. The salinity of the liquid in the lower chamber is adjusted by regulating the supply rates of both chambers. The first supply chamber 365 stores low-salinity liquids (such as clean water or slightly saline solution), and the second supply chamber 366 stores high-salinity liquids (such as concentrated saline solution). By adjusting the supply rates of both chambers, a liquid with the target salinity can be mixed in the lower chamber.

[0090] Based on the amount of organic acid secreted by the wild soybean roots as fed back by the measuring rod 3612, the system automatically adjusts the liquid supply ratio between the two chambers. For example, if an "overload" signal is detected, the supply of the first liquid supply chamber 365 is increased, and the supply of the second liquid supply chamber 366 is decreased, so that the salinity of the lower chamber drops to a range that the plant can tolerate.

[0091] In this embodiment, a second drain valve 3610 is provided in the upper compartment, and a first drain valve 369 is provided in the lower compartment.

[0092] When it is necessary to replace the liquid in the lower chamber (such as draining the old liquid after adjusting the salinity) or clean the lower chamber, open the first drain valve 369 to discharge the waste liquid to avoid concentration deviation caused by mixing liquids with different salinity.

[0093] Excess water may be generated in the upper storage area due to excessive spraying by the sprinkler 66 or soil expansion due to water absorption. Opening the second drain valve 3610 can drain the water accumulated in the upper storage area and prevent the soil from becoming too wet, which would affect the respiration of the wild soybean roots.

[0094] Furthermore, the second drain valve 3610 comes with a built-in screen.

[0095] It should also be explained that soil salinity and alkalinity can be adjusted in the following two ways:

[0096] The first method involves calculating the current soil salinity based on initial soil parameters (such as original salinity and soil bulk density) and parameters of the saline solution injected each time (such as concentration and supply volume), and then adjusting the subsequent saline solution supply strategy accordingly.

[0097] The second method involves installing a salinity sensor in the upper storage chamber to directly monitor the actual salinity of the soil, thereby adjusting the subsequent supply strategy of saline-alkali solution.

[0098] In addition, in scenarios where soil salinity needs to be reduced (which is relatively rare), clean water can be sprayed onto the soil layer through the sprayer 66 in the spray and supply component 6. The water flow dilutes the soil salts, and at the same time, saline wastewater is discharged through the second drain valve 3610, thus achieving an active reduction in salinity. This method complements the supply of saline solution for regulation, covering the salinity control needs throughout the entire experimental cycle.

[0099] In addition, the transfer of liquid from the lower compartment to the upper compartment can be achieved through the following two technical approaches:

[0100] The first method utilizes capillary action (such as the capillary force generated by the supply pipe 3613) to allow the saline solution in the lower chamber to rise naturally to the soil layer. Although this method can simulate the capillary rise process of saline solution in natural soil, the transport volume is affected by multiple factors such as soil porosity, liquid surface tension, and ambient temperature and humidity. It requires real-time calculation and adjustment of the supply parameters through complex models (such as the Darcy's law modified model), making control quite difficult.

[0101] The second method involves installing a miniature pump in the lower chamber to directly pump the brine solution from the lower chamber to the supply pipe 3613 via a pipeline. This method allows for real-time monitoring of the transmission volume using a flow sensor, enabling rapid and stable control of the transmission volume. It is simple to operate and highly reliable.

[0102] In actual experiments, the pump-based active transmission scheme is preferred.

[0103] In this embodiment, the bottom of the mesh cylinder 362 has a folded edge;

[0104] A hinge seat 364 is detachably connected to the partition 367. A locking claw 363 is hinged to the hinge seat 364. One end of the locking claw 363 abuts against the folded edge, and the other end abuts against the partition 367.

[0105] One end of the locking claw 363 abuts against the folded edge of the net cylinder 362, and the other end abuts against the partition plate 367, forming a "lever-type" clamp. Through leverage, the net cylinder 362 is pressed tightly against the partition plate 367, preventing displacement caused by spraying, rotation, or plant root growth. The hinge seat 364 is detachably connected to the partition plate 367 (e.g., via quick-release bolts). When the locking claw 363 is damaged or the net cylinder 362 needs to be replaced, the hinge seat 364 can be quickly disassembled for replacement or adjustment.

[0106] In this embodiment, a heating plate 368 is fixed below the partition 367. The heating plate 368 is fixed below the partition 367 and directly heats the partition 367. The heated partition 367 can increase the soil temperature, simulating the temperature environment of wild soybeans during their natural growth period (e.g., 20-25℃ is required during germination, and 25-30℃ is required during flowering).

[0107] In addition, the heating plate 368 can be a "ceramic heating plate" with heating wires evenly distributed on its surface and the power adjusted by a thermostat.

[0108] In this embodiment, a temperature and humidity sensor is provided on the inner wall of the cover 5;

[0109] A second gas tank 2 for supplying dry gas to the sealed space is also provided on one side of the cover 5.

[0110] The second gas tank 2 stores dry gas (such as dry air) and is connected to the casing 5 via a pipe. A solenoid valve controls the gas supply. When the humidity sensor detects that the humidity is too high, the control system opens the solenoid valve, and the second gas tank 2 injects dry gas into the sealed space to reduce the humidity to a suitable range. Once the humidity reaches the target range, the solenoid valve closes.

[0111] The temperature and humidity sensor can be a "digital temperature and humidity sensor (such as DHT22)" with a measurement range of -40 to 80℃ and 0 to 100%RH, and an accuracy of ±0.5℃ and ±2%RH, which can meet the experimental accuracy requirements.

[0112] The above description is merely a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A device for simulating and identifying the salt-alkali gradient tolerance of wild soybean throughout its entire growth period, characterized in that, include: Planting simulation component (3) is used to adaptively adjust salinity based on the growth characteristics of wild soybeans; A cover (5) is provided on the planting simulation component (3) to form a sealed space together with the planting simulation component (3). An exhaust pipe (7) is also provided on the cover (5). A first gas cylinder (1) is connected to the sealed space; The planting simulation component (3) includes: A base plate (31) together with the cover (5) forms the sealed space, and a drain pipe (4) is provided on the base plate (31); A toothed ring (32) is rotatably mounted on the base plate (31); Multiple circumferentially distributed rotary drive components (35) are embedded in the base plate (31), and each rotary drive component (35) has a rotary drive end. Multiple carrier components (36) are correspondingly installed on the rotary drive end, and the carrier components (36) are used as carriers for planting wild soybeans; A spraying and supplying assembly (6) is also fixed on the toothed ring (32), which is used to assist in supplying water or soil to the bearing assembly (36). The carrier component (36) includes: The carrying chamber (361) has a partition (367) fixed inside it to divide the carrying chamber (361) into an upper chamber and a lower chamber. The partition (367) has a through hole in the middle. A net cylinder (362) with a cylindrical plate (3611) fixed in the middle, the upper part of which is used to support soil and wild soybean seeds; A measuring rod (3612) passes through the cylinder plate (3611) and through the through hole. The measuring rod (3612) is used to measure the organic acid secretion of the wild soybean seeds and to measure the salinity of the liquid in the lower chamber. Multiple liquid supply pipes (3613) are distributed in a circular interval. The liquid supply pipes (3613) penetrate the partition (367). The bottom of the liquid supply pipe (3613) has a liquid inlet and the top has a liquid outlet. A seepage ring (3614) is installed at the liquid outlet.

2. The wild soybean salt-alkali gradient simulation identification device according to claim 1, characterized in that, The spraying and supply assembly (6) includes: Side seat (61), which is fixed to the toothed ring (32); A vertical drive assembly is disposed on the side seat (61), and the vertical drive assembly has a vertical drive end; The sprayer (66) and the soil supply chamber (67) are installed at the vertical drive end.

3. The wild soybean salt-alkali gradient simulation and identification device according to claim 1, characterized in that, The rotary drive assembly (35) includes: The outer ring (351) is embedded in the base plate (31); Multiple arc seats (353) are distributed in a circular interval, and the arc seats (353) are installed on the inner wall of the outer ring (351) by springs (352); A drive wheel (354) is rotatably mounted on the arc seat (353).

4. The wild soybean salt-alkali gradient simulation and identification device according to claim 1, characterized in that, Below the bearing chamber (361) are a first liquid supply chamber (365) and a second liquid supply chamber (366) for supplying liquid to the lower chamber. The salinity of the liquid in the lower chamber can be adjusted by adjusting the supply of the two chambers.

5. The wild soybean full-growth-cycle salt-alkali gradient simulation identification device according to claim 1, characterized in that, The upper chamber is equipped with a second drain valve (3610), and the lower chamber is equipped with a first drain valve (369).

6. The wild soybean salt-alkali gradient simulation and identification device according to claim 1, characterized in that, The bottom of the mesh tube (362) has a folded edge; A hinge seat (364) is detachably connected to the partition (367), and a locking claw (363) is hinged to the hinge seat (364). One end of the locking claw (363) abuts against the folded edge, and the other end abuts against the partition (367).

7. The wild soybean salt-alkali gradient simulation and identification device according to claim 1, characterized in that, A heating plate (368) is fixed below the partition (367).

8. The wild soybean salt-alkali gradient simulation identification device according to claim 1, characterized in that, A temperature and humidity sensor is provided on the inner wall of the cover (5); A second gas tank (2) for supplying dry gas to the sealed space is also provided on one side of the cover (5).

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