Flow path system for gene sequencing, control method of flow path system and gene sequencer

Abstract

A flow path system for gene sequencing, comprising: a sequencing slide (13) comprising a plurality of mutually independent sample channels (C1-C4); at least one of: a biological sample loading flow path configured to be able to load different biological samples in at least two sample channels, respectively, and / or selectively load one biological sample into a portion of the plurality of sample channels; a sequencing reagent loading flow path configured to selectively load a plurality of different sequencing reagents into a portion of the sample channel, respectively; and a cleaning flow path configured to be able to clean components in the flow path system using a plurality of cleaning agents, respectively, therefore, different samples are respectively loaded in the at least two sample channels, and various different cleaning agents are used for respectively cleaning each flow path component in the flow path system.

Description

Flow path system for gene sequencing, control method thereof, and gene sequencer Technical Field

[0001] The present disclosure relates to the technical field of gene sequencers, and in particular to a flow system for gene sequencing, an operating method thereof, and a gene sequencer. Background Art

[0002] The test slide in a gene sequencer is divided into at least two sample channels. In theory, each independent channel can be loaded with a different biological sample. However, due to the complexity of the fluidic system, existing equipment does not have the function of loading different biological samples into each sample channel separately. This requires a dedicated loading tool and cannot automatically use multiple different cleaning agents to clean each flow path component. This makes the cleaning process inefficient and time-consuming.

[0003] Summary of the Invention

[0004] The present disclosure provides a flow path system for gene sequencing, comprising: a sequencing slide including multiple independent sample channels; and at least one of the following: a biological sample loading flow path for loading different biological samples into at least two sample channels, respectively, and / or selectively loading a biological sample into a portion of the multiple sample channels; a sequencing reagent loading flow path for selectively loading multiple different sequencing reagents into a portion of the sample channels, respectively; and a cleaning flow path for cleaning components in the flow path system using multiple cleaning agents, respectively.

[0005] In some embodiments, the flow system further includes at least one of the following: a biological sample area, provided with a plurality of sample storage chambers for storing a variety of different biological samples; a sequencing reagent area, storing a variety of different sequencing reagents; a cleaning agent area, storing a variety of different cleaning agents for cleaning the flow path components of the flow system; and a waste liquid area, connected to the biological sample loading flow path, the sequencing reagent loading flow path, and the cleaning flow path to receive waste liquid.

[0006] In some embodiments, the flow path system includes: a suction device, including multiple suction components that work independently of each other and are used to aspirate biological samples; a first reversing valve assembly, including multiple first reversing valves that work independently of each other; and three ports of each first reversing valve are respectively connected to a corresponding biological sample storage chamber, a corresponding suction component and a corresponding sample channel to achieve selective communication of the suction component between the biological sample storage chamber and the sample channel.

[0007] In some embodiments, the first port of each first reversing valve is connected to a corresponding biological sample storage chamber, the second port of each first reversing valve is connected to the I port of a corresponding suction component, and the third port of each first reversing valve is connected to the inlet of a corresponding sample channel. When the first reversing valve is energized, the first port is connected to the second port, and the second port and the third port are disconnected. When the first reversing valve is de-energized, the second port and the third port are connected, and the first port and the second port are disconnected.

[0008] In some embodiments, the flow path system also includes a rotary valve and a fifth reversing valve, the first port of the fifth reversing valve is connected to the rotary valve, the second port of the fifth reversing valve is connected to a corresponding biological sample storage bin, and the third port of the fifth reversing valve is connected to the first port of a reversing valve among multiple first reversing valves; the rotary valve is connected to the outlet ends of multiple sample channels and to the waste liquid area. When the fifth reversing valve is energized, the first port and the second port are connected, and the second port and the third port are disconnected. When the fifth reversing valve is de-energized, the first port and the second port are disconnected, and the second port and the third port are connected.

[0009] In some embodiments, each O port of the plurality of suction components is connected to the waste liquid area so that the waste liquid can be discharged into the waste liquid area when the plurality of suction components are switched to the O port.

[0010] The present disclosure also provides a gene sequencer, comprising the above-mentioned flow system.

[0011] The present disclosure also provides a method for controlling a flow system for gene sequencing, wherein the flow system includes a biological sample area, a first reversing valve assembly, a sequencing slide, a suction device, and a waste liquid area. The biological sample area is provided with a plurality of sample storage bins for accommodating a plurality of biological samples respectively. The first reversing valve assembly includes a plurality of first reversing valves. The sequencing slide is provided with a plurality of sample channels. The suction device includes a plurality of suction components. The first ports of the plurality of first reversing valves are respectively connected to the plurality of sample storage bins through a plurality of first pipelines. The second ports of the plurality of first reversing valves are respectively connected to the I ports of the plurality of suction components through a plurality of second pipelines. The third port of the first reversing valve is connected to the inlet ends of the multiple sample channels through multiple third pipelines, and a rotary valve is provided between the outlet ends of the multiple sample channels and the waste liquid area to control the connection and disconnection between the outlet ends of the multiple sample channels and the waste liquid area; the method includes: energizing the multiple first reversing valves so that the first ports of the multiple first reversing valves are connected to the second ports respectively; generating negative pressure at the I ports of the multiple suction components to respectively draw the multiple samples from the multiple sample storage bins through the first pipeline, the multiple first reversing valves, and the second pipeline to the I ports of the multiple suction components, and finally temporarily storing the multiple biological samples in the second pipeline.

[0012] The present disclosure also provides a method for controlling a flow system for gene sequencing, wherein the flow system includes a biological sample area, a first reversing valve assembly, a sequencing slide, a suction device and a waste liquid area, wherein a plurality of sample storage chambers for respectively accommodating a plurality of biological samples are provided in the biological sample area, the first reversing valve assembly includes a plurality of first reversing valves, the sequencing slide is provided with a plurality of sample channels, the suction device includes a plurality of suction components, the first ports of the plurality of first reversing valves are respectively connected to the plurality of sample storage chambers through a plurality of first pipelines, the second ports of the plurality of first reversing valves are respectively connected to the I ports of the plurality of suction components through a plurality of second pipelines, the third ports of the plurality of first reversing valves are respectively connected to the inlet ends of the plurality of sample channels through a plurality of third pipelines, and a rotary valve is provided between the outlet ends of the plurality of sample channels and the waste liquid area to control the flow between the outlet ends of the plurality of sample channels and the waste liquid area. The method is characterized in that the first reversing valve assembly of the flow path system includes a second reversing valve, the first port of the second reversing valve is connected to the first sequencing reagent needle in the sequencing reagent area, the second port of the second reversing valve is connected to the B port of the multiple suction components, and the third port of the second reversing valve is respectively connected to the outlet end of the multiple sample channels and the rotary valve through a three-way connection. When each of the first and second reversing valves is energized, the first port is connected to the second port, and the second port and the third port are disconnected. When each of the first and second reversing valves is de-energized, the second port is connected to the third port, and the first port and the second port are disconnected. The method comprises the following steps: the multiple first reversing valves are de-energized and the second reversing valves are energized; and the multiple suction components provide negative pressure, so that the sequencing reagent passes from the sequencing reagent area through the second reversing valve and the B port of the multiple suction components and enters the multiple suction components for temporary storage.

[0013] The present disclosure also provides a method for controlling a flow system for gene sequencing, wherein the flow system includes a biological sample area, a first reversing valve assembly, a sequencing slide, a suction device and a waste liquid area, wherein a plurality of sample storage chambers for respectively accommodating a plurality of biological samples are provided in the biological sample area, the first reversing valve assembly includes a plurality of first reversing valves, the sequencing slide is provided with a plurality of sample channels, the suction device includes a plurality of suction components, the first ports of the plurality of first reversing valves are respectively connected to the plurality of sample storage chambers through a plurality of first pipelines, the second ports of the plurality of first reversing valves are respectively connected to the I ports of the plurality of suction components through a plurality of second pipelines, the third ports of the plurality of first reversing valves are respectively connected to the inlet ends of the plurality of sample channels through a plurality of third pipelines, a rotary valve is provided on the pipeline between the outlet ends of the plurality of sample channels and the waste liquid area, for controlling the on-off between the outlet ends of the plurality of sample channels and the waste liquid area; wherein the first reversing valve assembly of the flow system further includes a second reversing valve, the first port of the second reversing valve is connected to the first sequencing reagent needle in the sequencing reagent area, the second port of the second reversing valve is connected to the B ports of the plurality of suction components, the third port of the second reversing valve is connected to the inlet ends of the plurality of sample channels The ports are connected to the outlet ends of the plurality of sample channels and the rotary valves through three-way joints; a fifth reversing valve is provided in a first pipeline between one of the plurality of first reversing valves and a corresponding sample storage chamber, the first port of the fifth reversing valve being connected to the rotary valve, the second port of the fifth reversing valve being connected to the fourth sample storage chamber, and the third port of the fifth reversing valve being connected to the first port of one of the plurality of first reversing valves. When each of the first, second, and fifth reversing valves is energized, the first port is connected to the second port, and the second port and the third port are disconnected; when each of the first, second, and fifth reversing valves is de-energized, the second port is connected to the third port, and the first port and the second port are disconnected. The method comprises the following steps: energizing the plurality of first reversing valves and the fifth reversing valve and de-energizing the second reversing valve; one of the plurality of suction components providing negative pressure to aspirate the biological sample from a sample storage chamber, the sample sample passing through the sample needle, the fifth reversing valve, the rotary valve, and the second reversing valve in sequence to reach port B of the plurality of suction components, at which point the fifth pipeline between the rotary valve and the outlet ends of the plurality of sample channels is filled with the biological sample.

[0014] The present disclosure provides a method for controlling a flow path system for gene sequencing, wherein the flow path system includes a biological sample area, a first reversing valve assembly, a sequencing slide, a suction device, and a waste liquid area. A plurality of sample storage bins are provided in the biological sample area to respectively accommodate a plurality of biological samples. The first reversing valve assembly includes a plurality of first reversing valves. The sequencing slide is provided with a plurality of sample channels. The suction device includes a plurality of suction components. The first ports of the plurality of first reversing valves are respectively connected to the plurality of sample storage bins through a plurality of first pipelines. The second ports of the plurality of first reversing valves are respectively connected to the I ports of the plurality of suction components through a plurality of second pipelines. The third port is connected to the inlet ends of the multiple sample channels respectively through multiple third pipelines, and a rotary valve is provided between the outlet ends of the multiple sample channels and the waste liquid area to control the on-off between the outlet ends of the multiple sample channels and the waste liquid area; wherein the first reversing valve assembly of the flow path system further comprises a second reversing valve, the first port of the second reversing valve is connected to the first sequencing reagent needle in the sequencing reagent area, the second port of the second reversing valve is connected to the B port of the multiple suction components, and the third port of the second reversing valve is connected to the outlet ends of the multiple sample channels and the rotary valve respectively through a three-way connection; the sequencing reagent area is provided with multiple second sequencing reagent needles, respectively corresponding to the multiple Different sequencing reagents, the rotary valve is provided with a plurality of holes, respectively connected to a plurality of second sequencing reagent needles, when the rotary valve is switched to one of the hole positions, the rotary valve will be connected to one of the second sequencing reagent needles corresponding to the hole position, when each of the first and second reversing valves is powered, the first port is connected to the second port, and the second port and the third port are disconnected, when each of the first and second reversing valves is powered off, the second port is connected to the third port, and the first port and the second port are disconnected; the method includes at least one of a bypass operation and a byship operation: the bypass operation includes the following steps: (1) The plurality of first reversing valves are energized and the second reversing valves are de-energized, the rotary valve is switched to connect with the desired sequencing reagent through a second sequencing reagent needle, the plurality of suction components are switched to port B, negative pressure is provided to suck the desired sequencing reagent from the sequencing reagent area, and the sequencing reagent finally enters the plurality of suction components through the second sequencing reagent needle, the rotary valve, the fifth pipeline, the second reversing valve, and the port B of the plurality of suction components; (2) the sequencing reagent enters the plurality of suction components, or as long as it passes through the second reversing valve; (3) the plurality of suction components are switched to port O, positive pressure is provided to transfer the sequencing reagent from the plurality of suction components to the waste liquid area via the fourth pipeline;The Bychip operation includes the following steps: (1) the plurality of first reversing valves lose power, the second reversing valves gain power, the rotary valves are switched to communicate with the desired sequencing reagent through a second sequencing reagent needle, the plurality of suction components are switched to port I, the plurality of suction components provide negative pressure to push the desired sequencing reagent through the second sequencing reagent needle, the rotary valve, the fifth channel and the outlet end of the sequencing slide into the biological channel, replacing the old sequencing reagent therein, and the old sequencing reagent and the excess new sequencing reagent are discharged from the inlet end of the sequencing slide, through the third pipeline, the plurality of suction components, the second pipeline and the port I of the plurality of suction components, and enter the plurality of suction components; (2) the plurality of suction components are switched to port O, providing positive pressure thrust to push out the sequencing reagent inside thereof, and discharge it into the waste liquid area through the fourth pipeline.

[0015] The present disclosure provides a method for controlling a flow system for gene sequencing, wherein the flow system includes a biological sample area, a first reversing valve assembly, a sequencing slide, a suction device, a waste liquid area, and a cleaning agent area. The biological sample area is provided with a plurality of sample storage bins for accommodating a plurality of biological samples respectively. The first reversing valve assembly includes a plurality of first reversing valves and a second reversing valve. The sequencing slide is provided with a plurality of sample channels. The suction device includes a plurality of suction components. The first ports of the plurality of first reversing valves are respectively connected to the plurality of sample storage bins through a plurality of first pipelines. The second ports of the plurality of first reversing valves are respectively connected to the I ports of the plurality of suction components through a plurality of second pipelines. The third ports of the plurality of first reversing valves are respectively connected to the I ports of the plurality of suction components through a plurality of third pipelines. The first and second reversing valves are connected to the inlet ends of the multiple sample channels, respectively. A rotary valve is provided between the outlet ends of the multiple sample channels and the waste liquid area to control the on-off between the outlet ends of the multiple sample channels and the waste liquid area. The first port of the second reversing valve is connected to the first sequencing reagent needle in the sequencing reagent area, the second port of the second reversing valve is connected to the B port of the multiple suction components, and the third port of the second reversing valve is connected to the outlet ends of the multiple sample channels and the rotary valve through a three-way connection. A fifth reversing valve is provided on the first pipeline between one of the multiple first reversing valves and a corresponding sample storage bin. The first port of the fifth reversing valve is connected to the rotary valve, and the second port of the fifth reversing valve is connected to a sample storage bin. The third port of the fifth reversing valve is connected to the first port of a reversing valve; the flow path system also includes a cleaning agent area and a second valve group, the cleaning agent area stores a variety of different types of cleaning agents, the second valve group includes multiple inlets and a common outlet, the multiple inlets are respectively connected to the multiple different types of cleaning agents, the common outlet can be selectively connected to the outer wall of the second sequencing reagent needle and the pipeline between the second reversing valve and the B port of the multiple suction components; the common outlet of the second valve group is connected to the second port of the fourth reversing valve, the third port of the fourth reversing valve is connected to the inlet of the pump, the outlet of the pump is connected to the second port of the third reversing valve, the third port of the third reversing valve is connected to the outer wall of the multiple second sequencing reagent needles, and the The first ports of the third reversing valve and the fourth reversing valve are connected, and the pipeline between the first port of the third reversing valve and the first port of the fourth reversing valve is selectively connected to the pipeline between the second reversing valve and the B port of the plurality of suction components through the stop valve. When each of the first to fifth reversing valves is energized, the first port is connected to the second port, and the second port and the third port are disconnected. When each of the first to fifth reversing valves is de-energized, the second port is connected to the third port, and the first port and the second port are disconnected. The method includes at least one of the following steps: Step a: When one of the two-way valves is turned on, a corresponding cleaning agent is selected. If the cleaning agent is air, it can be used to empty the liquid in the pipeline;Step b: The third reversing valve and the fourth reversing valve are de-energized, and the pump rotates forward to transport the selected cleaning agent from the cleaning agent area, through the second valve group, the fourth reversing valve, the pump, and the third reversing valve to the outer wall of the second sequencing reagent needle in the sequencing reagent area, thereby cleaning the outer wall of the second sequencing reagent needle; Step c: The third reversing valve is energized and the fourth reversing valve is de-energized, and the pump rotates forward to extract the required cleaning agent, pass through the second valve group, the fourth reversing valve, the pump, the third reversing valve, and reach the stop valve; Step d: The stop valve is connected, multiple first reversing valves are energized and the second reversing valve is de-energized, and the selected cleaning agent passes through the stop valve, the second reversing valve, the fifth pipeline, the rotary valve, the second sequencing reagent needle in sequence. , and finally enters the sequencing reagent tank in the sequencing reagent area; step e: the rotary valve is switched to each hole position in sequence, and step d is repeated to complete the cleaning of the inner wall of each second sequencing reagent needle and its connecting pipeline; step f: step a is performed multiple times to select different cleaning agents respectively, and step e is performed to clean the inner wall of each second sequencing reagent needle and its connecting pipeline multiple times with different cleaning agents; step g: the third reversing valve is energized and the fourth reversing valve is de-energized, multiple first reversing valves are energized and the second reversing valves are connected, the pump rotates in the opposite direction, and one inlet of the second valve group is connected to the waste liquid area, and the waste liquid in the sequencing reagent tank in the sequencing reagent area is sucked into the waste liquid area through one inlet; step h: cut off The valve is connected, multiple first reversing valves are energized and the second reversing valve is de-energized, the rotary valve is switched to a blocked hole position, the B and O ports of multiple suction components are opened at the same time, and the cleaning agent output in step c passes through the multi-liquid dividing block, the B and O ports of multiple suction components in sequence, and is finally discharged from the waste liquid area, completing the cleaning of related pipelines and valves; step i: the third reversing valve is de-energized and the fourth reversing valve is energized, the pump is closed, and the selected cleaning agent passes through the outlet of the second valve group and the fourth reversing valve to reach the stop valve; step j: the stop valve is connected, multiple first reversing valves and the second reversing valve are de-energized, the rotary valve is switched to a designated blocked hole position, and multiple suction components are switched to port I to provide negative pressure suction cleaning. The cleaning agent passes through the stop valve, the second reversing valve, the multiple biological channels of the test slide, the multiple first reversing valves, the I ports of the multiple suction components, and finally enters the interior of the multiple suction components to complete the cleaning of the relevant pipelines and valves; step k: the multiple suction components are switched to the O port, and positive pressure is provided to discharge the cleaning agent therein into the waste liquid area through the fourth pipeline; step l: the stop valve is turned on, the multiple first reversing valves are energized, the second reversing valve is de-energized, the rotary valve 15 is switched to a designated blocked hole position, and the multiple suction components are switched to the B port to provide negative pressure to suck the cleaning agent, which passes through the stop valve, the one-point multi-point liquid block, the B ports of the multiple suction components, and finally enters the interior of the multiple suction components for temporary storage;Step m: Multiple first reversing valves are energized, and the second reversing valves are de-energized. Multiple suction components are switched to port I, providing positive pressure to push the cleaning agent, pushing out the cleaning agent temporarily stored in the multiple suction components in steps j and i. The cleaning agent passes through the ports I of the multiple suction components and the multiple first reversing valves, and finally passes through the multiple sample needles to be discharged into the multiple sample storage bins in the sample storage area, completing the cleaning of the relevant pipes and valves. Step n: The rotary valve is switched to a hole position connected to the fifth reversing valve. Multiple first reversing valves are energized, and the second reversing valves are de-energized. The fifth reversing valve is energized, and multiple suction components are switched to port B. Positive pressure is provided to push out the cleaning agent temporarily stored in the multiple suction components in steps j or l. The cleaning agent passes through the ports B of the multiple suction components, the one-split multi-liquid block, the second reversing valve, the rotary valve, the fifth reversing valve, and finally passes through the sample needle to be discharged into a sample storage bin, completing the cleaning of the relevant pipes and valves.

[0016] The flow path system for gene sequencing disclosed herein can achieve at least one of the following technical effects: different biological samples can be independently loaded into multiple sample channels of the same sequencing slide to better meet the sequencing requirements of different biological samples; multiple different sequencing reagents can be selectively loaded into selected sample channels to meet the need for using different sequencing reagents in sequencing; and multiple different cleaning agents can be used to clean the flow path components in the flow path system to meet the different cleaning requirements of each component. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] FIG1 is a first exemplary diagram of a flow system for gene sequencing disclosed herein;

[0018] FIG2 is a second exemplary diagram of a fluid system for gene sequencing according to the present disclosure;

[0019] FIG3 is a third exemplary diagram of a fluid system for gene sequencing according to the present disclosure;

[0020] FIG4 is a fourth exemplary diagram of a fluid system for gene sequencing according to the present disclosure;

[0021] FIG5 is a fifth exemplary diagram of a fluid system for gene sequencing according to the present disclosure;

[0022] FIG6 is a sixth exemplary diagram of a fluid system for gene sequencing according to the present disclosure;

[0023] FIG7 is a seventh exemplary diagram of a fluid system for gene sequencing according to the present disclosure;

[0024] FIG8 is an eighth exemplary diagram of a fluid system for gene sequencing according to the present disclosure;

[0025] FIG9 is a ninth exemplary diagram of a fluid system for gene sequencing according to the present disclosure;

[0026] FIG10 is a tenth exemplary diagram of a fluid system for gene sequencing according to the present disclosure;

[0027] FIG11 is an eleventh exemplary diagram of a fluid system for gene sequencing according to the present disclosure;

[0028] FIG12 is a twelfth exemplary diagram of a fluid system for gene sequencing according to the present disclosure;

[0029] FIG13 is a thirteenth exemplary diagram of a fluid system for gene sequencing according to the present disclosure;

[0030] FIG14 is a fourteenth exemplary diagram of a fluid system for gene sequencing according to the present disclosure;

[0031] FIG15 is a fifteenth exemplary diagram of a fluid system for gene sequencing according to the present disclosure; and

[0032] FIG. 16 is a sixteenth exemplary diagram of a fluid system for gene sequencing according to the present disclosure. DETAILED DESCRIPTION

[0033] The present disclosure will be further described in detail below in conjunction with specific embodiments. The examples given are only for illustrating the present disclosure but not for limiting the scope of the present disclosure.

[0034] Loading flow path

[0035] The disclosed flow system 10 for gene sequencing includes a biological sample area 11, a first reversing valve assembly 12, a sequencing slide 13, and an aspiration device 14. The biological sample area 11 is provided with multiple sample storage compartments for accommodating different biological samples, and the sequencing slide 13 is provided with multiple independent sample channels. The first reversing valve assembly 12 is in communication with the biological sample area 11, the sequencing slide 13, and the aspiration device 14, respectively. The aspiration device 14 is switchably connected between the biological sample area 11 and the sequencing slide 13 through the first reversing valve assembly 12, thereby aspirating and transferring different biological samples into the multiple sample channels of the sequencing slide 13. The biological sample area 11 can be divided into a plurality of independent sample storage compartments as needed; the dividing outlines are not shown in the figures.

[0036] The present disclosure will be described below using four components as an example. It will be understood that the above-mentioned embodiments of the present disclosure may also select more or fewer components, and each component includes at least a sample storage chamber, a reversing valve, a suction component of a suction device, and a sample channel, etc. It is also possible to select more or fewer of the components to selectively load biological samples into some sample channels. The "multiple" here means at least two, and the "first" and "second" are only for textual distinction and do not have any limiting effect. "Connected" means that they are connected in the flow path.

[0037] In some embodiments, as shown in FIG1 , the biological sample area 11 is provided with first to fourth sample storage chambers for accommodating first to fourth biological samples respectively, the first reversing valve assembly 12 includes four first reversing valves T1-T4, the sequencing slide 13 is provided with four independent first to fourth sample channels C1-C4, the suction device 14 includes first to fourth suction components P1-P4, the first ports of the four first reversing valves T1-T4 are connected to the first to fourth sample storage chambers through four first pipelines L1 respectively through four sample needles, and the four The second ports of the first reversing valves T1-T4 are connected to the inlets (Inlets) of the first to fourth suction components P1-P4 through four second pipelines L2, and the third ports of the four first reversing valves T1-T4 are connected to the inlets of the first to fourth sample channels C1-C4 through four third pipelines L3. A rotary valve 15 is provided between the outlets of the first to fourth sample channels C1-C4 and the waste liquid area 16 to control the flow between the outlets of the first to fourth sample channels C1-C4 and the waste liquid area 16. Optionally, the first reversing valve assembly 12 includes four three-way solenoid valves and a base manifold. Optionally, the suction device 14 includes four suction pumps, each of which includes a syringe and a valve head, and the syringe can be selectively connected to one of the multiple interfaces of the valve head.

[0038] In some embodiments, different biological samples are placed in the first to fourth sample storage chambers, respectively.

[0039] In some embodiments, as shown in FIG1 , the four first reversing valves T1-T4 are energized, that is, the first ports of the four first reversing valves T1-T4 are connected to the second ports respectively, and the second ports are disconnected from the third ports. The I ports of the first to fourth suction components P1-P4 provide negative pressure to draw the first to fourth samples from the first to fourth sample storage bins, through the first pipeline L1, the four first reversing valves T1-T4, and the second pipeline L2 to the I ports of the first to fourth suction components P1-P4 respectively. Finally, the biological samples (for example, DNB1-DNB4) are temporarily stored in the four second pipelines L2 respectively.

[0040] In some embodiments, as shown in FIG2 , the four first reversing valves T1-T4 lose power, the second ports of the four first reversing valves T1-T4 are connected to the third ports respectively, the first ports and the second ports are disconnected, and the first to fourth suction components P1-P4 provide positive pressure to push the biological samples temporarily stored in the second pipeline L2 through the four first reversing valves T1-T4, the third pipeline L3, and the first to fourth sample channels C1-C4. The excess biological samples are discharged from the outlet ends of the first to fourth sample channels C1-C4 and enter the waste liquid area 16 through the rotary valve 15.

[0041] In some embodiments, as shown in FIG3 , the disclosed flow system for gene sequencing includes a sequencing reagent area 17 for storing sequencing reagents. The first reversing valve assembly 12 includes a second reversing valve T5. The first port of the second reversing valve T5 communicates with a first sequencing reagent needle 171 in the sequencing reagent area 17. The first sequencing reagent needle 171 communicates with a sequencing reagent, such as an auxiliary reagent. The second port of the second reversing valve T5 communicates with ports B of the first to fourth pumping components P1-P4. The third port of the second reversing valve T5 communicates via a tee with the outlets of the first to fourth sample channels C1-C4 and the rotary valve 15, respectively. The sequencing reagent area 17 can be divided into several independent reagent storage compartments as needed; the dividing outlines are not shown in the figure.

[0042] In some embodiments, as shown in FIG3 , the four first reversing valves T1-T4 are de-energized, the second reversing valve T5 is energized, and the first to fourth suction components P1-P4 provide negative pressure, allowing the sequencing reagents to flow from the sequencing reagent area 17 through the second reversing valve T5 and the ports B of the first to fourth suction components P1-P4 and enter the first to fourth suction components P1-P4 for temporary storage to expel air from the pipelines; the O ports (Outlet) of the first to fourth suction components P1-P4 are connected to the waste liquid area 16 via four fourth pipelines L4.

[0043] In some embodiments, as shown in FIG4 , when the first to fourth suction components P1-P4 are switched to port O, the first to fourth suction components P1-P4 provide a positive pressure driving force to discharge the sequencing reagents temporarily stored in the first to fourth suction components P1-P4 into the waste liquid area 16 through the fourth pipeline L4.

[0044] In some embodiments, the four first reversing valves T1-T4 are energized, the second reversing valve T5 is de-energized, and the first to fourth suction components P1-P4 are switched to port I (Inlet), providing positive pressure to push the sequencing reagents temporarily stored in the first to fourth suction components P1-P4 through the second pipeline L2, the four first reversing valves T1-T4, and the first pipeline L1 to reach the sample needles in the biological sample area 11, thereby filling the first and second pipelines L1 and L2 with sequencing reagents and exhausting the air in the pipelines. Please refer to Figure 1 for the flow path.

[0045] In some embodiments, the four first reversing valves T1-T4 are de-energized, the second reversing valve T5 is energized, the rotary valve 15 is switched to a position communicating with the waste liquid area 16, and the first to fourth pumping components P1-P4 are switched to port I. This provides positive pressure to push the sequencing reagents temporarily stored in the first to fourth pumping components P1-P4 through the second line L2, the four first reversing valves T1-T4, the third line L3, the first to fourth sample channels C1-C4, the fifth line L5, the rotary valve 15, and finally into the waste liquid area 16. This replaces the old sequencing reagents in the first to fourth sample channels C1-C4. The replaced old sequencing reagents and excess sequencing reagents are discharged from the outlets of the first to fourth sample channels C1-C4 to the waste liquid area 16, preventing contamination of the sequencing reagents in the first to fourth sample channels C1-C4 during subsequent testing steps. The flow diagram is similar to that in FIG2 .

[0046] In some embodiments, biological samples are added only to the fourth biological storage compartment.

[0047] In some embodiments, as shown in FIG5 , a fifth reversing valve T9 is provided on the first pipeline L1 between the first reversing valve T4 and the fourth sample storage chamber. The first port of the fifth reversing valve T9 is connected to the rotary valve 15, the second port of the fifth reversing valve T9 is connected to the fourth sample storage chamber, and the third port of the fifth reversing valve T9 is connected to the first port of the first reversing valve T4. When the four first reversing valves T1-T4 and T9 are energized and the second reversing valve T5 is de-energized, the fourth suction component P4 applies negative pressure to aspirate the fourth biological sample from the fourth sample storage chamber. The sample is then drawn through the sample needle, the fifth reversing valve T9, the rotary valve 15, and the second reversing valve T5 to ports B of the first to fourth suction components P1-P4. At this point, the fifth pipeline L5 between the rotary valve 15 and the outlets of the first to fourth sample channels C1-C4 is filled with the fourth biological sample, thereby purging and filling the pipeline with the biological sample.

[0048] In some embodiments, as shown in FIG6 , the fifth reversing valve T9 is energized, the four first reversing valves T1-T4 are de-energized, and the second reversing valve T5 is energized. The first to fourth suction components P1-P4 are switched to port I, providing negative pressure to draw the fourth biological sample in the fifth line L5 from the outlet of the sequencing slide 13 into the first to fourth biological sample channels C1-C4, respectively. Excess fourth biological sample is discharged from the inlet of the sequencing slide 13, passes through the third line L3, the four first reversing valves T1-T4, the second line L2, and through port I of the first to fourth suction components P1-P4 into the first to fourth suction components P1-P4, thereby completing the loading of the fourth biological sample into the first to fourth sample channels C1-C4. Referring to FIG4 , the first to fourth suction components P1-P4 are switched to port O, providing positive pressure to push the biological sample in the first to fourth suction components P1-P4 through the fourth line L4 into the waste liquid area 16.

[0049] In some embodiments, as shown in FIG7 , sequencing reagent area 17 further includes multiple second sequencing reagent needles 172 , each corresponding to a plurality of different sequencing reagents. Rotary valve 15 includes multiple apertures, each communicating one-to-one with each of the plurality of second sequencing reagent needles 172 . When rotary valve 15 is switched to one aperture, rotary valve 15 communicates with the second sequencing reagent needle 172 corresponding to that aperture.

[0050] In some embodiments, as shown in FIG7 , a sequencing reagent pre-treatment device 24 is provided in the pipeline between the plurality of second sequencing reagent needles 172 and the rotary valve 15 , and has at least one of filtering, removing dissolved oxygen, and degassing the sequencing reagent drawn from the sequencing reagent area 17 .

[0051] There are two ways to deliver sequencing reagents: Bypass and Bychip.

[0052] The steps for Bypass are as follows:

[0053] (1) As shown in FIG7 , the four first reversing valves T1-T4 are energized, the second reversing valve T5 is de-energized, the rotary valve 15 is switched to communicate with the desired sequencing reagent through a second sequencing reagent needle 172, the first to fourth suction components P1-P4 are switched to port B, providing negative pressure to draw the desired sequencing reagent from the sequencing reagent area 17, and the desired sequencing reagent finally enters the first to fourth suction components P1-P4 after passing through the second sequencing reagent needle 172, the rotary valve 15, the fifth pipeline L5, the second reversing valve T5, and the port B of the first to fourth suction components P1-P4;

[0054] (2) The sequencing reagents may also enter the first to fourth pumping components P1-P4, or may exceed the second reversing valve T5, as long as the sequencing reagents completely fill the fifth pipeline L5;

[0055] (3) The first to fourth suction components P1-P4 are switched to port O, providing positive pressure to transfer the sequencing reagents from the first to fourth suction components P1-P4 to the waste liquid area 16 via the fourth pipeline L4.

[0056] The operation steps of Bychip are as follows:

[0057] (1) As shown in FIG8 , the four first reversing valves T1-T4 lose power, the second reversing valve T5 gains power, the first rotary valve 15 is switched to connect with the required sequencing reagent through a second sequencing reagent needle 172, the first to fourth suction components P1-P4 are switched to port I, the first to fourth suction components P1-P4 provide negative pressure to move the required sequencing reagent through the second sequencing reagent needle 172, the rotary valve 15, the fifth channel L5 and the outlet end of the sequencing slide 13 into the biological channel C1-C4, replacing the old sequencing reagent therein, the old sequencing reagent and the excess new sequencing reagent are discharged from the inlet end of the sequencing slide 13, through the third pipeline L3, the first to fourth suction components P1-P4, the second pipeline L2 and port I of the first to fourth suction components P1-P4, and enter the first to fourth suction components P1-P4.

[0058] (2) The first to fourth suction components P1-P4 switch to port O, providing positive pressure to push out the sequencing reagents inside, and discharge them into the waste liquid area 16 through the fourth pipeline L4.

[0059] You can take one of the following steps:

[0060] 1. Follow all the steps of Bypass, then follow all the steps of Bychip;

[0061] 2. Execute steps (1) and (2) of Bypass, and then execute steps (1) and (2) of Bychip;

[0062] Execute steps (1) and (2) of Bypass, then execute step (1) of Bychip, rotate valve 15 to switch to another desired sequencing reagent, then execute steps (1) and (2) of Bypass, and then execute steps (1) and (2) of Bychip.

[0063] The loading flow path disclosed herein can bring at least one of the following technical effects:

[0064] Different biological samples can be loaded independently in multiple sample channels of the same sequencing slide to better meet the sequencing needs of different biological samples and improve the flexibility of gene sequencing;

[0065] A plurality of different sequencing reagents are selectively loaded into the selected sample channels respectively to meet the need of using different sequencing reagents in different channels in gene sequencing.

[0066] Cleaning flow path

[0067] In some embodiments, as shown in FIG9 , the flow system for gene sequencing further includes a cleaning flow path, comprising a cleaning agent region 18 and a second valve assembly 19. Cleaning agent region 18 stores multiple different types of cleaning agents, such as a first cleaning agent W1, a second cleaning agent W2, a third cleaning agent W3, a fourth cleaning agent W4, and a fifth cleaning agent W5. Second valve assembly 18 includes multiple inlets and a common outlet, each of which communicates with the different types of cleaning agents. For example, second valve assembly 19 includes four two-way valves T10-T13, each having an inlet, each communicating with a corresponding cleaning agent. The outlets of each two-way valve are connected to form a common outlet, which selectively communicates with the pipeline between the second reversing valve T5 and ports B of the first to fourth pumping components P1-P4, and the outer walls of multiple second sequencing reagent needles 172. Cleaning agent region 18 can be divided into several independent cleaning agent storage compartments as needed; the dividing outlines are not shown in the figure.

[0068] In some embodiments, as shown in Figure 9, the cleaning flow path includes a second reversing valve assembly, which is respectively connected to the common outlet of the second valve group 19, the pipeline between the second reversing valve T5 and the B port of the first to fourth suction parts P1-P4, and the outer walls of multiple second sequencing reagent needles 172, so as to selectively connect the cleaning agent from the common outlet of the second valve group 19 to the pipeline between the second reversing valve T5 and the B port of the first to fourth suction parts P1-P4 and one of the outer walls of the multiple second sequencing reagent needles 172.

[0069] In some embodiments, as shown in Figure 9, the second reversing valve assembly includes a third reversing valve T7, a fourth reversing valve T8 and a pump 21, the common outlet of the second valve group 19 is connected to the second port of the fourth reversing valve T8, the third port of the fourth reversing valve T8 is connected to the inlet of the pump 21, and the outlet of the pump 21 is connected to the second port of the third reversing valve T7, the third port of the third reversing valve T7 is connected to the outer wall of the plurality of second sequencing reagent needles 172, the third reversing valve T7 is connected to the first port of the fourth reversing valve T8, and the pipeline between the first port of the third reversing valve T7 and the first port of the fourth reversing valve T8 is selectively connected to the pipeline between the second reversing valve T5 and the B ports of the first to fourth suction components P1-P4 through the stop valve T6. Optionally, a multi-liquid distributor 22 is provided in the pipeline between the third port of the third reversing valve T7 and the outer walls of the plurality of second sequencing reagent needles 172. This distributor 22 distributes the cleaning agent from the third port of the third reversing valve T7 to the outer walls of the selected second sequencing reagent needles 172 for cleaning. Optionally, the pump 21 can be speed-adjustable and capable of forward and reverse rotation, and may include, for example, a diaphragm pump or a peristaltic pump.

[0070] In some embodiments, as shown in FIG9 , the second valve group 19 further includes another two-way valve T14 , the inlet of the two-way valve T14 is connected to the waste liquid area 16 , and the outlet of the two-way valve T14 is connected to the common outlet of the second valve group 19 ;

[0071] Clean the flow path by performing at least one of the following steps:

[0072] Step a: One of the two-way valves is selected to be connected, and the corresponding cleaning agent is selected. If the cleaning agent is air, it can be used to empty the liquid in the pipeline.

[0073] Step b: As shown in FIG9 , the third reversing valve T7 and the fourth reversing valve T8 are de-energized, and the pump 21 rotates forward to transport the selected cleaning agent from the cleaning agent area 18 , through the second valve group 19 , the fourth reversing valve T8 , the pump 21 , and the third reversing valve T7 , to the outer wall of the second sequencing reagent needle 172 in the sequencing reagent area 17 , thereby cleaning the outer wall of the second sequencing reagent needle 172 .

[0074] Step c: As shown in Figure 10, the third reversing valve T7 is energized and the fourth reversing valve T8 is de-energized, and the pump 21 rotates forward so that the required cleaning agent is extracted and passes through the second valve group 19, the fourth reversing valve T8, the pump 21, and the third reversing valve T7 to reach the stop valve T6.

[0075] Step d: As shown in 10 , the cutoff valve T6 is connected, the four first reversing valves T1-T4 are energized, and the second reversing valve T5 is de-energized. The selected cleaning agent passes through the cutoff valve T6, the second reversing valve T5, the fifth pipeline L5, the rotary valve 15, the second sequencing reagent needle 172, and finally enters the sequencing reagent tank of the sequencing reagent area 17.

[0076] Step e: The rotary valve 15 is switched to each well position in sequence, and step d is repeated to complete the cleaning of the inner wall of each second sequencing reagent needle 172 and its connecting pipeline.

[0077] Step f: Perform step a multiple times, select different cleaning agents respectively, and perform step e to clean the inner wall of each second sequencing reagent needle 172 and its connecting pipe multiple times with different cleaning agents;

[0078] Step g: As shown in FIG11 , the third reversing valve T7 is energized and the fourth reversing valve T8 is de-energized. The four first reversing valves T1 to T4 are energized and the second reversing valve T5 is de-energized. The pump 21 rotates in the reverse direction, and only the other two-way valve T14 of the second valve group is connected. The waste liquid in the sequencing reagent tank of the sequencing reagent area 17 is sucked into the waste liquid area 16.

[0079] Step h: As shown in Figure 12, the stop valve T6 is turned on, the reversing valves T1-4 are energized and the second reversing valve T5 is de-energized, the rotary valve 15 is switched to a blocked hole position, and the B and O ports of the first to fourth suction components P1-P4 are opened at the same time. The cleaning agent output in step c passes through the first to fourth liquid dividing blocks, the B and O ports of the first to fourth suction components P1-P4 in sequence, and is finally discharged from the waste liquid area 16, completing the cleaning of the relevant pipelines and valves.

[0080] Step i: As shown in FIG13 , the third reversing valve T7 is de-energized and the fourth reversing valve T8 is energized, the pump 21 is turned off, and the selected cleaning agent passes through the outlet of the second valve group 19 and the fourth reversing valve T8 to reach the stop valve T6;

[0081] Step j: As shown in Figure 13, the stop valve T6 is turned on, the first to second reversing valves T1-T5 are de-energized, the rotary valve 15 is switched to a designated blocked hole, and the first to fourth suction components P1-P4 are switched to port I to provide negative pressure to suction the cleaning agent, which passes through the stop valve T6, the second reversing valve T5, the four biological channels C1-C4 of the test slide 13, the four first reversing valves T1-T4, the port I of the first to fourth suction components P1-P4, and finally enters the interior of the first to fourth suction components P1-P4 to complete the cleaning of the relevant pipes and valves.

[0082] Step k: The first to fourth suction components P1 - P4 are switched to port O to provide positive pressure to discharge the cleaning agent therein into the waste liquid area 16 through the fourth pipeline L4.

[0083] Step 1: As shown in Figure 14, the stop valve T6 is turned on, the four first reversing valves T1-T4 are energized, the second reversing valve T5 is de-energized, the rotary valve 15 is switched to a designated blocked hole position, and the first to fourth suction components P1-P4 are switched to port B, providing negative pressure to suck the cleaning agent, which passes through the stop valve T6, the one-fourth liquid block 23, the B ports of the first to fourth suction components P1-P4, and finally enters the first to fourth suction components P1-P4 for temporary storage.

[0084] Step m: As shown in Figure 15, the four first reversing valves T1-T4 are energized and the second reversing valve T5 is de-energized, and the first to fourth suction components P1-P4 are switched to port I, providing positive pressure to push the cleaning agent, and the cleaning agent temporarily stored in the first to fourth suction components P1-P4 in steps j and i is pushed out, in turn, through the port I of the first to fourth suction components P1-P4 and the four first reversing valves T1-T4, and finally through the four sample needles to be discharged into the first to fourth sample storage bins in the sample storage area 11, completing the cleaning of the relevant pipelines and valves.

[0085] Step n: As shown in Figure 16, the rotary valve 15 is switched to the hole position connected to the fifth reversing valve T9, the four first reversing valves T1-4 are energized and the second reversing valve T5 is de-energized, the fifth reversing valve T9 is energized, and the first to fourth suction components P1-P4 are switched to port B, providing positive pressure to push out the cleaning agent temporarily stored in the first to fourth suction components P1-P4 in step j or l, and pass through the B ports of the first to fourth suction components P1-P4, the one-fourth liquid block, the second reversing valve T5, the rotary valve 15, the fifth reversing valve T9, and finally through a sample needle to be discharged to the fourth sample storage bin, completing the cleaning of related pipelines and valves.

[0086] According to the cleaning flow path disclosed in the present invention, a variety of different cleaning agents can be used to clean the flow path components in the flow path system to meet the different cleaning requirements of each component, improve cleaning efficiency, save labor, and achieve better cleaning effects.

[0087] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present disclosure, rather than to limit them. Although the present disclosure has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or perform equivalent replacements on some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present disclosure.

Claims

1. A flow system for gene sequencing, comprising: A sequencing slide (13), comprising a plurality of independent sample channels (C1-C4); as well as At least one of the following: A biological sample loading flow path, configured to be capable of loading different biological samples into at least two sample channels respectively, and / or selectively loading a biological sample into a portion of the plurality of sample channels; A sequencing reagent loading flow path, configured to selectively load a plurality of different sequencing reagents into a portion of the sample channel; as well as The cleaning flow path is configured to be able to use a plurality of cleaning agents to clean the components in the flow path system respectively.

2. The flow path system according to claim 1, further comprising at least one of the following: A biological sample area (11) is provided with a plurality of sample storage compartments for storing a variety of different biological samples; A sequencing reagent area (17), storing a variety of different sequencing reagents; a cleaning agent area (18) storing a plurality of different cleaning agents for cleaning the flow path components of the flow path system; and The waste liquid area (16) is connected to the biological sample loading flow path, the sequencing reagent loading flow path and the cleaning flow path so as to receive the waste liquid.

3. The flow path system according to claim 2, comprising: A suction device (14) comprising a plurality of suction components (P1-P4) operating independently of each other, for suctioning biological samples; A first reversing valve assembly (12) includes a plurality of first reversing valves (T1-T4) that operate independently of each other; and The three ports of each first reversing valve are respectively connected to a corresponding biological sample storage chamber, a corresponding suction component and a corresponding sample channel to achieve selective communication between the suction component and the biological sample storage chamber and the sample channel.

4. The flow path system according to claim 3, wherein the first port of each first reversing valve is connected to a corresponding biological sample storage bin, the second port of each first reversing valve is connected to port I of a corresponding suction component, and the third port of each first reversing valve is connected to an inlet of a corresponding sample channel; when the first reversing valve is energized, the first port is connected to the second port, and the second port is disconnected from the third port; when the first reversing valve is de-energized, the second port is connected to the third port, and the first port is disconnected from the second port.

5. The flow path system according to claim 4 further includes a rotary valve (15) and a fifth reversing valve T9, wherein the first port of the fifth reversing valve T9 is connected to the rotary valve (15), the second port of the fifth reversing valve T9 is connected to a corresponding biological sample storage bin, and the third port of the fifth reversing valve T9 is connected to the first port of one of the multiple first reversing valves; the rotary valve (15) is connected to the outlet ends of the multiple sample channels and to the waste liquid area (16); when the fifth reversing valve T9 is energized, the first port and the second port are connected, and the second port and the third port are disconnected; when the fifth reversing valve T9 is de-energized, the first port and the second port are disconnected, and the second port and the third port are connected.

6. A flow path system according to any one of claims 3 to 5, wherein each O port of the multiple suction components (P1-P4) is connected to the waste liquid area (16) so that the multiple suction components (P1-P4) can discharge the waste liquid into the waste liquid area (16) when switching to the O port.

7. The flow path system according to any one of claims 3 to 6, wherein the first reversing valve assembly (12) further comprises a second reversing valve T5, a first port of the second reversing valve T5 being connected to a first sequencing reagent needle (171) of the sequencing reagent area (17), a second port of the second reversing valve T5 being connected to each B port of the plurality of suction components via a one-to-many liquid dividing block (23), and a third port of the second reversing valve T5 being connected to the rotary valve (15) and the outlet ends of the plurality of sample channels respectively via a three-way connection.

8. The flow path system according to any one of claims 5 to 7, wherein the sequencing reagent area (17) is provided with a plurality of second sequencing reagent needles (172), and the plurality of second sequencing reagent needles (172) are respectively connected to a plurality of different sequencing reagents in the sequencing reagent area (17), and the rotary valve (15) has a plurality of hole positions, each of the plurality of hole positions is connected to a corresponding second sequencing reagent needle (172), and the rotary valve (15) can be switched to any one of the plurality of hole positions so as to be connected to a corresponding second sequencing reagent needle (172).

9. The flow system according to claim 8, wherein a sequencing reagent pre-treatment device (24) is provided on the pipeline between the second sequencing reagent needle (172) and the rotary valve (15), for performing at least one of filtering, removing dissolved oxygen, and degassing on the sequencing reagent from the sequencing reagent area (17).

10. The flow path system according to claim 8 or 9 comprises a second valve group (19) and a second reversing valve assembly (20), wherein the second valve group (19) comprises a plurality of inlets and a common outlet, wherein the plurality of inlets are respectively connected to a plurality of cleaning agents, and any one of the plurality of inlets can be connected to obtain a desired cleaning agent, and the common outlet is selectively connected to one of the outer walls of the second sequencing reagent needle (172) of the sequencing reagent area (17) through the second reversing valve assembly (20) and the pipeline between the second reversing valve T5 and the B ports of the plurality of suction components.

11. The flow path system according to claim 10, wherein the second reversing valve assembly comprises a third reversing valve T7, a fourth reversing valve T8 and a pump (21), the common outlet of the second valve group (19) is connected to the second port of the fourth reversing valve T8, the third port of the fourth reversing valve T8 is connected to the inlet of the pump (21), the outlet of the pump (21) is connected to the second port of the third reversing valve T7, the third port of the third reversing valve T7 is connected to the outer wall of a plurality of second sequencing reagent needles (172), the third reversing valve T7 is connected to the first port of the fourth reversing valve T8, the pipeline between the first port of the third reversing valve T7 and the first port of the fourth reversing valve T8 is selectively connected to the pipeline between the second reversing valve T5 and the B port of a plurality of suction components through a stop valve T6, when the seventh and fourth reversing valves are powered, the first port and the second port are connected, and the second port and the third port are disconnected, when the seventh and fourth reversing valves are powered off, the first port and the second port are disconnected, and the second port and the third port are connected.

12. The flow path system according to claim 11, wherein a multi-liquid distribution block (22) is provided in the pipeline between the third port of the third reversing valve T7 and the outer walls of the plurality of second sequencing reagent needles (172) so as to distribute the cleaning agent from the third reversing valve T7 to the outer walls of the selected second sequencing reagent needles (172).

13. The flow path system according to claim 10, wherein the second valve group (19) comprises a plurality of first two-way valves, the inlet of each first two-way valve being respectively connected to different cleaning agents in the cleaning agent area (18), and the outlet of each first two-way valve being connected to each other.

14. The flow path system according to any one of claims 10 to 13, wherein the second valve group (19) further comprises a second two-way valve T14, an inlet of the second two-way valve T14 being connected to the waste liquid area (16), and an outlet of the second two-way valve T14 being connected to outlets of a plurality of first two-way valves, so that when the pump (21) is reversed, the waste liquid in the sequencing reagent area (17) can be discharged to the waste liquid area (16) via the inlet of the second two-way valve T14.

15. A gene sequencer comprising the flow system described in any one of claims 1-14.

16. A method for controlling a flow path system for gene sequencing, the flow path system comprising a biological sample area (11), a first reversing valve assembly (12), a sequencing slide (13), a suction device (14) and a waste liquid area (16), wherein the biological sample area (11) is provided with a plurality of sample storage bins for accommodating a plurality of biological samples respectively, the first reversing valve assembly (12) comprises a plurality of first reversing valves (T1-T4), the sequencing slide (13) is provided with a plurality of sample channels (C1-C4), the suction device (14) comprises a plurality of suction components (P1-P4), and the first ports of the plurality of first reversing valves (T1-T4) are connected to the reversing valve assembly (12). The plurality of sample storage bins are respectively connected through a plurality of first pipelines L1, the second ports of the plurality of first reversing valves (T1-T4) are respectively connected with the I ports of the plurality of suction components (P1-P4) through a plurality of second pipelines L2, the third ports of the plurality of first reversing valves (T1-T4) are respectively connected with the inlet ends of the plurality of sample channels (C1-C4) through a plurality of third pipelines L3, and a rotary valve (15) is provided between the outlet ends of the plurality of sample channels (C1-C4) and the waste liquid area (16) to control the connection and disconnection between the outlet ends of the plurality of sample channels (C1-C4) and the waste liquid area (16); the method comprises: The plurality of first reversing valves (T1-T4) are energized so that the first ports of the plurality of first reversing valves (T1-T4) are respectively connected to the second ports; The I ports of the multiple suction components (P1-P4) generate negative pressure to draw the multiple samples from the multiple sample storage bins through the first pipeline L1, the multiple first reversing valves (T1-T4), and the second pipeline L2 to the I ports of the multiple suction components. Finally, the multiple biological samples are temporarily stored in the second pipeline L2.

17. The method according to claim 16, comprising: The plurality of first reversing valves (T1-T4) are de-energized, and the second ports of the plurality of first reversing valves (T1-T4) are respectively connected to the third Port connectivity; A plurality of suction components (P1-P4) provide positive pressure to push the biological samples temporarily stored in the second pipeline L2 through a plurality of first reversing valves (T1-T4), a third pipeline L3, and a plurality of sample channels (C1-C4). Excess biological samples are discharged from the outlet ends of the plurality of sample channels (C1-C4) and enter the waste liquid area (16) through a rotary valve (15).

18. A method for controlling a flow path system for gene sequencing, the flow path system comprising a biological sample area (11), a first reversing valve assembly (12), a sequencing slide (13), a suction device (14) and a waste liquid area (16), wherein a plurality of sample storage bins for respectively accommodating a plurality of biological samples are provided in the biological sample area (11), the first reversing valve assembly (12) comprises a plurality of first reversing valves (T1-T4), the sequencing slide (13) comprises a plurality of sample channels (C1-C4), the suction device (14) comprises a plurality of suction components (P1-P4), the first ports of the plurality of first reversing valves (T1-T4) are respectively connected to the plurality of sample storage bins through a plurality of first pipelines L1, the second ports of the plurality of first reversing valves (T1-T4) are respectively connected to the I ports of the plurality of suction components (P1-P4) through a plurality of second pipelines L2, and the third ports of the plurality of first reversing valves (T1-T4) are respectively connected to the plurality of sample channels (C1-C4) through a plurality of third pipelines L3. The inlet ends of the plurality of sample channels (C1-C4) are connected, and a rotary valve (15) is provided between the outlet ends of the plurality of sample channels (C1-C4) and the waste liquid area (16) to control the opening and closing between the outlet ends of the plurality of sample channels (C1-C4) and the waste liquid area (16); wherein the first reversing valve assembly (12) of the flow path system comprises a second reversing valve T5, the first port of the second reversing valve T5 is connected to the first sequencing reagent needle (171) of the sequencing reagent area (17), the second port of the second reversing valve T5 is connected to the B ports of the plurality of suction components P1-P4, the third port of the second reversing valve T5 is connected to the outlet ends of the plurality of sample channels and the rotary valve (15) through a three-way connection, when each of the first and second reversing valves is powered, the first port is connected to the second port, and the second port is disconnected from the third port, and when each of the first and second reversing valves is powered off, the second port is connected to the third port, and the first port is disconnected from the second port; the method comprises the following steps: Multiple first reversing valves (T1-T4) lose power, and the second reversing valve T5 gains power; as well as The multiple suction components (P1-P4) provide negative pressure, so that the sequencing reagents pass from the sequencing reagent area (17) through the second reversing valve T5 and the B ports of the multiple suction components (P1-P4) and enter the multiple suction components (P1-P4) for temporary storage.

19. The method according to claim 18, wherein in the flow path system, the O ports of the plurality of suction components (P1-P4) are connected to the waste liquid area (16) through four fourth pipelines L4 respectively; the method comprises: When the multiple suction components are switched to port O, the multiple suction components provide a positive pressure driving force to discharge the sequencing reagents temporarily stored in the multiple suction components (P1-P4) into the waste liquid area (16) through the fourth pipeline L4.

20. The method according to claim 18 or 19, comprising the steps of: The plurality of first reversing valves are powered and the second reversing valve T5 is de-energized; The plurality of suction components are switched to port I to provide positive pressure to push the sequencing reagents temporarily stored in the plurality of suction components (P1-P4) through the second pipeline L2, the plurality of first reversing valves (T1-T4), and the first pipeline L1 to reach the biological sample area (11) Each sample needle is inserted into the first and second tubes L1 and L2, thereby filling the sequencing reagents.

21. The method according to claim 20, comprising the steps of: De-energize the plurality of first reversing valves (T1-T4) and energize the second reversing valve T5; The rotary valve (15) is switched to a hole position connected to the waste liquid area (16), and the multiple suction components (P1-P4) are switched to port I, providing positive pressure to push the sequencing reagents temporarily stored in the multiple suction components (P1-P4), and pass through the second pipeline L2, the multiple first reversing valves (T1-T4), the third pipeline L3, the multiple sample channels (C1-C4), the fifth pipeline L5, the rotary valve (15), and finally enter the waste liquid area (16).

22. A method for controlling a flow path system for gene sequencing, the flow path system comprising a biological sample area (11), a first reversing valve assembly (12), a sequencing slide (13), a suction device (14) and a waste liquid area (16), wherein the biological sample area (11) is provided with a plurality of sample storage bins for respectively accommodating a plurality of biological samples, the first reversing valve assembly (12) comprises a plurality of first reversing valves (T1-T4), the sequencing slide (13) is provided with a plurality of sample channels (C1-C4), the suction device (14) comprises a plurality of suction components (P1-P4), the plurality of first reversing valves (T1 The first ports of the plurality of first reversing valves (T1-T4) are respectively connected to the plurality of sample storage bins through a plurality of first pipelines L1, the second ports of the plurality of first reversing valves (T1-T4) are respectively connected to the I ports of the plurality of suction components (P1-P4) through a plurality of second pipelines L2, the third ports of the plurality of first reversing valves (T1-T4) are respectively connected to the inlet ends of the plurality of sample channels (C1-C4) through a plurality of third pipelines L3, and a rotary valve (15) is provided on the pipeline between the outlet ends of the plurality of sample channels (C1-C4) and the waste liquid area (16) to control the outlets of the plurality of sample channels (C1-C4) The first reversing valve assembly (12) of the flow path system further comprises a second reversing valve T5, wherein the first port of the second reversing valve T5 is connected to the first sequencing reagent needle (171) of the sequencing reagent area (17), the second port of the second reversing valve T5 is connected to the B port of the plurality of suction components (P1-P4), and the third port of the second reversing valve T5 is respectively connected to the outlet end of the plurality of sample channels (C1-C4) and the rotary valve (15) through a three-way connection; the first pipeline between a reversing valve T4 in the plurality of first reversing valves and a corresponding sample storage bin A fifth reversing valve T9 is provided on L1, wherein the first port of the fifth reversing valve T9 is connected to the rotary valve (15), the second port of the fifth reversing valve T9 is connected to the fourth sample storage bin, and the third port of the fifth reversing valve T9 is connected to the first port of a reversing valve T4 among the plurality of first reversing valves. When each of the first, second and fifth reversing valves is powered, the first port is connected to the second port, and the second port is disconnected from the third port. When each of the first, second and fifth reversing valves is powered off, the second port is connected to the third port, and the first port is disconnected from the second port. The method comprises the following steps: The plurality of first reversing valves (T1-T4) and the fifth reversing valve T9 are powered and the second reversing valve T5 is de-energized; A suction component (P4) of the plurality of suction components provides negative pressure to suck the biological sample out of a sample storage bin, and the sample sample passes through the sample needle, the fifth reversing valve T9, the rotary valve (15) and the second reversing valve T5 in sequence to reach the B port of the plurality of suction components (P1-P4). At this time, the fifth pipeline L5 between the rotary valve (15) and the outlet end of the plurality of sample channels (C1-C4) is filled. The biological sample.

23. The method according to claim 22, comprising the steps of: The fifth reversing valve T9 is energized, the first reversing valves (T1-T4) are de-energized, and the second reversing valve T5 is energized; The multiple suction components (P1-P4) are switched to port I, providing negative pressure to suck the biological sample in the fifth pipeline L5 from the outlet end of the sequencing slide (13) into the multiple biological sample channels (C1-C4) respectively, and the excess biological sample is discharged from the inlet end of the sequencing slide (13), passes through the third pipeline L3, the multiple first reversing valves (T1-T4), the second pipeline L2, and enters the multiple suction components (P1-P4) through port I of the multiple suction components (P1-P4).

24. A method for controlling a flow path system for gene sequencing, the flow path system comprising a biological sample area (11), a first reversing valve assembly (12), a sequencing slide (13), a suction device (14) and a waste liquid area (16), wherein the biological sample area (11) is provided with a plurality of sample storage bins for accommodating a plurality of biological samples respectively, the first reversing valve assembly (12) comprises a plurality of first reversing valves (T1-T4), the sequencing slide (13) is provided with a plurality of sample channels (C1-C4), the suction device (14) comprises a plurality of suction components (P1-P4), and the plurality of first reversing valves (T1-T4) are provided. The first ports of the valves (T1-T4) are respectively connected to the plurality of sample storage bins through a plurality of first pipelines L1, the second ports of the plurality of first reversing valves (T1-T4) are respectively connected to the I ports of the plurality of suction components (P1-P4) through a plurality of second pipelines L2, the third ports of the plurality of first reversing valves (T1-T4) are respectively connected to the inlet ends of the plurality of sample channels (C1-C4) through a plurality of third pipelines L3, and a rotary valve (15) is provided between the outlet ends of the plurality of sample channels (C1-C4) and the waste liquid area (16) for controlling the outlet ends of the plurality of sample channels (C1-C4) and the waste liquid area (16). 4) and the waste liquid area (16); wherein the first reversing valve assembly (12) of the flow path system further comprises a second reversing valve T5, the first port of the second reversing valve T5 is in communication with the first sequencing reagent needle (171) of the sequencing reagent area (17), the second port of the second reversing valve T5 is in communication with the B ports of the plurality of suction components (P1-P4), the third port of the second reversing valve T5 is in communication with the outlet ends of the plurality of sample channels (C1-C4) and the rotary valve (15) through a three-way connection; the sequencing reagent area (17) is provided with a plurality of second sequencing reagent needles, which respectively correspond to For multiple different sequencing reagents, the rotary valve (15) is provided with multiple hole positions, which are respectively connected with multiple second sequencing reagent needles (172). When the rotary valve (15) is switched to one of the hole positions, the rotary valve (15) will be connected with one of the second sequencing reagent needles (172) corresponding to the hole position. When each of the first and second reversing valves is powered, the first port is connected with the second port, and the second port is disconnected from the third port. When each of the first and second reversing valves is powered off, the second port is connected with the third port, and the first port and the second port are disconnected. The method includes at least one of a Bypass operation and a Byship operation: The Bypass operation includes the following steps: (1) The plurality of first reversing valves (T1-T4) are energized and the second reversing valve T5 is de-energized, the rotary valve (15) is switched to be connected to the desired sequencing reagent through a second sequencing reagent needle (172), the plurality of suction components (P1-P4) are switched to port B, negative pressure is provided to suck the desired sequencing reagent from the sequencing reagent area (17), and the second sequencing reagent needle (172), the rotary valve (15) and the reversing valve (15) are connected to the sequencing reagent area (17). The rotary valve (15), the fifth pipeline L5, the second reversing valve T5, the B port of the plurality of suction components (P1-P4), and the sequencing reagent finally enters the plurality of suction components (P1-P4); (2) The sequencing reagents enter the multiple pumping components (P1-P4), or just pass through the second reversing valve T5; (3) the plurality of suction components (P1-P4) are switched to port O to provide positive pressure to transfer the sequencing reagents from the plurality of suction components (P1-P4) to the waste liquid area (16) via the fourth pipeline L4; and Bychip operation includes the following steps: (1) The plurality of first reversing valves (T1-T4) lose power, the second reversing valve T5 gains power, the rotary valve (15) is switched to communicate with the required sequencing reagent through a second sequencing reagent needle (172), the plurality of suction components (P1-P4) are switched to port I, the plurality of suction components (P1-P4) provide negative pressure to move the required sequencing reagent through the second sequencing reagent needle (171), the rotary valve (15), the fifth channel L5 and the outlet end of the sequencing carrier (13) into the biological channel (C1-C4), replacing the old sequencing reagent therein, the old sequencing reagent and the excess new sequencing reagent are discharged from the inlet end of the sequencing carrier (13), through the third pipeline L3, the plurality of suction components (P1-P4), the second pipeline L2 and port I of the plurality of suction components (P1-P4), and enter the plurality of suction components (P1-P4); (2) Switch the O ports of the multiple suction components (P1-P4) to provide positive pressure to push out the sequencing reagents inside, and discharge them into the waste liquid area (16) through the fourth pipeline L4.

25. The method according to claim 24: Include one of the following steps: Execute all the steps of Bypass, and then execute all the steps of Bychip; Execute steps (1) and (2) of Bypass, and then execute steps (1) and (2) of Bychip; as well as Execute steps (1) and (2) of Bypass, then execute step (1) of Bychip, rotate valve (15) to switch to another desired sequencing reagent, then execute steps (1) and (2) of Bypass, and then execute steps (1) and (2) of Bychip.

26. A method for controlling a flow path system for gene sequencing, the flow path system comprising a biological sample area (11), a first reversing valve assembly (12), a sequencing slide (13), an aspiration device (14), a waste liquid area (16) and a cleaning agent area (18), the biological sample area (11) being provided with a plurality of sample storage bins for accommodating a plurality of biological samples respectively, the first reversing valve assembly (12) comprising a plurality of first reversing valves (T1-T4) and a second reversing valve T5, the sequencing slide (13) being provided with The plurality of sample channels (C1-C4), the suction device (14) comprises a plurality of suction components (P1-P4), the first ports of the plurality of first reversing valves (T1-T4) are respectively connected to the plurality of sample storage bins through a plurality of first pipelines L1, the second ports of the plurality of first reversing valves (T1-T4) are respectively connected to the I ports of the plurality of suction components (P1-P4) through a plurality of second pipelines L2, the third ports of the plurality of first reversing valves (T1-T4) are respectively connected to the plurality of sample storage bins through a plurality of third pipelines L3 The inlet ends of the channels (C1-C4) are connected, and a rotary valve (15) is provided between the outlet ends of the multiple sample channels (C1-C4) and the waste liquid area (16) to control the connection and disconnection between the outlet ends of the multiple sample channels (C1-C4) and the waste liquid area (16); wherein the first port of the second reversing valve T5 is connected to the first sequencing reagent needle (171) of the sequencing reagent area (17), the second port of the second reversing valve T5 is connected to the B port of the multiple suction components (P1-P4), and the second reversing valve T5 is connected to the B port of the multiple suction components (P1-P4). The third port of the valve T5 is respectively connected to the outlet ends of the plurality of sample channels (C1-C4) and the rotary valve (15) through a three-way connection; a fifth reversing valve T9 is provided on the first pipeline L1 between a reversing valve T4 among the plurality of first reversing valves and a corresponding sample storage bin, the first port of the fifth reversing valve T9 is connected to the rotary valve (15), the second port of the fifth reversing valve T9 is connected to a sample storage bin, and the third port of the fifth reversing valve T9 is connected to the first port of the reversing valve T4; The flow path system also includes a cleaning agent area (18) and a second valve group (19), wherein the cleaning agent area (18) stores a plurality of different types of cleaning agents, and the second valve group (19) includes a plurality of inlets and a common outlet, wherein the plurality of inlets are respectively connected to the plurality of different types of cleaning agents, and the common outlet can be selectively connected to the outer wall of the second sequencing reagent needle (172) and the pipeline between the second reversing valve T5 and the B ports of the plurality of suction components (P1-P4); The common outlet of the second valve group (19) is connected to the second port of the fourth reversing valve T8, the third port of the fourth reversing valve T8 is connected to the inlet of the pump (21), the outlet of the pump (21) is connected to the second port of the third reversing valve T7, the third port of the third reversing valve T7 is connected to the outer wall of the plurality of second sequencing reagent needles (172), the third reversing valve T7 is connected to the first port of the fourth reversing valve T8, the pipeline between the first port of the third reversing valve T7 and the first port of the fourth reversing valve T8 is selectively connected to the pipeline between the second reversing valve T5 and the B port of the plurality of suction components (P1-P4) through the stop valve T6, when each of the first to fifth reversing valves is powered, the first port is connected to the second port, and the second port is disconnected from the third port, when each of the first to fifth reversing valves is powered off, the second port is connected to the third port, and the first port is disconnected from the second port; The method comprises at least one of the following steps: Step a: Connect one of the two-way valves and select the corresponding cleaning agent. If the cleaning agent is air, it can be used to drain the liquid in the pipeline; Step b: the third reversing valve T7 and the fourth reversing valve T8 lose power, and the pump (21) rotates forward to transport the selected cleaning agent from the cleaning agent area (18) through the second valve group (19), the fourth reversing valve T8, the pump (21), and the third reversing valve T7 to the outer wall of the second sequencing reagent needle (172) in the sequencing reagent area (17), so as to clean the outer wall of the second sequencing reagent needle (172); Step c: the third reversing valve T7 is energized and the fourth reversing valve T8 is de-energized, and the pump (21) rotates in the forward direction so that the required cleaning agent is extracted and passes through the second valve group (19), the fourth reversing valve T8, the pump (21), the third reversing valve T7 to reach the stop valve T6; Step d: When the stop valve T6 is turned on, the first reversing valves (T1-T4) are energized and the second reversing valve T5 is de-energized, and the selected cleaning agent passes through the stop valve T6, the second reversing valve T5, the fifth pipeline L5, the rotary valve (15), the second sequencing reagent needle (172), and finally enters the sequencing reagent tank of the sequencing reagent area (17); Step e: the rotary valve (15) is switched to each hole position in turn, and step d is repeated to complete the cleaning of the inner wall of each second sequencing reagent needle (172) and its connecting pipeline; Step f: performing step a multiple times to select different cleaning agents respectively, and performing step e to use different cleaning agents to clean the inner wall of each second sequencing reagent needle (172) and its connecting pipeline multiple times; Step g: the third reversing valve T7 is energized and the fourth reversing valve T8 is de-energized, the plurality of first reversing valves (T1-T4) are energized and the second reversing valve T5, the pump (21) rotates in the opposite direction, and one inlet of the second valve group (19) is connected to the waste liquid area (16), and the waste liquid in the sequencing reagent tank of the sequencing reagent area (17) is sucked into the waste liquid area (16) through the one inlet; Step h: the stop valve T6 is turned on, the first reversing valves (T1-T4) are energized and the second reversing valve T5 is de-energized, the rotary valve (15) is switched to a blocked hole position, the B and O ports of the plurality of suction components (P1-P4) are opened simultaneously, and the cleaning agent output in step c passes through the one-point-multiple liquid dividing block (23), the B and O ports of the plurality of suction components (P1-P4) in sequence, and is finally discharged from the waste liquid area (16), thereby completing the cleaning of the relevant pipelines and valves; Step i: the third reversing valve T7 loses power and the fourth reversing valve T8 gains power, the pump (21) is turned off, and the selected cleaning agent passes through the outlet of the second valve group (19), the fourth reversing valve T8, and reaches the stop valve T6; Step j: the shut-off valve T6 is turned on, the first reversing valves (T1-T4) and the second reversing valve T5 are de-energized, the rotary valve (15) is switched to a designated blocked hole, the plurality of suction components (P1-P4) are switched to port I, and negative pressure is provided to suck the cleaning agent, which passes through the shut-off valve T6, the second reversing valve T5, the plurality of biological channels (C1-C4) of the test slide (13), the plurality of first reversing valves (T1-T4), the port I of the plurality of suction components (P1-P4), and finally enters the interior of the plurality of suction components (P1-P4), thereby completing the cleaning of the relevant pipelines and valves; Step k: multiple suction components (P1-P4) are switched to port O to provide positive pressure to discharge the cleaning agent therein into the waste liquid area (16) through the fourth pipeline L4; Step 1: the stop valve T6 is turned on, the first reversing valves (T1-T4) are energized, the second reversing valve T5 is de-energized, the rotary valve 15 is switched to a designated blocked hole, the plurality of suction components (P1-P4) are switched to the B port, and negative pressure is provided to suck the cleaning agent, which passes through the stop valve T6, the one-dividing-multi-dividing liquid block (23), the B ports of the plurality of suction components (P1-P4) in sequence, and finally enters the plurality of suction components (P1-P4) for temporary storage; Step m: the plurality of first reversing valves (T1-T4) are energized, the second reversing valve T5 is de-energized, the plurality of suction components (P1-P4) are switched to port I, positive pressure is provided to push the cleaning agent, and the cleaning agent temporarily stored in the plurality of suction components (P1-P4) in steps j and i is pushed out, and in turn the ports I of the plurality of suction components (P1-P4) and the plurality of first reversing valves T1-T4 are discharged into the plurality of sample storage bins in the sample storage area (11) through the plurality of sample needles, thereby completing the cleaning of the relevant pipelines and valves; Step n: the rotary valve (15) is switched to the hole position connected to the fifth reversing valve T9, the first reversing valves (T1-T4) are energized and the second reversing valve T5 is de-energized, the fifth reversing valve T9 is energized, and the suction components (P1-P4) are switched to the B port. Positive pressure is provided to push out the cleaning agent temporarily stored in the plurality of suction components (P1-P4) in step j or l, and the cleaning agent passes through the B ports of the plurality of suction components (P1-P4), the one-dividing-multi-dividing liquid block (23), the second reversing valve T5, the rotary valve (15), the fifth reversing valve T9, and finally is discharged to a sample storage bin through the sample needle, thereby completing the cleaning of the relevant pipelines and valves.

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