基于数字PCR检测HIV-1核酸的引物探针组合和检测方法
By designing primer-probe combinations based on digital PCR, the problems of limited detection types and insufficient sensitivity of existing HIV nucleic acid detection kits have been solved. This enables coverage detection of major prevalent HIV-1 subtypes both domestically and internationally, improving detection sensitivity and specificity, and shortening the detection window period.
CN120945122BActive Publication Date: 2026-07-17深圳市宝安区疾病预防控制中心(深圳市宝安区卫生监督所)
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- 深圳市宝安区疾病预防控制中心(深圳市宝安区卫生监督所)
- Filing Date
- 2025-07-25
- Publication Date
- 2026-07-17
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Figure CN120945122B_ABST
Abstract
本发明公开了基于数字PCR检测HIV‑1核酸的引物探针组合和检测方法。该引物探针组合包括：(1)POL区引物探针组合：序列如SEQ ID NO.1和SEQ ID NO.2所示的POL区引物及序列如SEQ ID NO.3所示的POL区探针；(2)LTR区引物探针组合：序列如SEQ ID NO.4和SEQ ID NO.5所示的LTR区引物及序列如SEQ ID NO.6所示的POL区探针；(3)GAG区引物探针组合：序列如SEQ ID NO.7和SEQ ID NO.8所示的GAG区下游引物及序列如SEQ ID NO.9所示的GAG区探针。该引物探针组合能够实现对目前HIV‑1主要流行型别的覆盖检测。
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