Moisturizing and repairing composition as well as preparation method and application thereof

Abstract

The invention discloses a moisturizing and repairing composition as well as a preparation method and application thereof. The moisturizing and repairing composition is prepared from the following components in percentage by weight: 3.0 to 10.0 percent of European glauca buds, 3.0 to 10.0 percent of spanishneedles herb, 2.0 to 10.0 percent of spirulina maxima, 2.0 to 10.0 percent of raspberry fruit, 0.5 to 5.0 percent of aconite leaves and cowpea seeds, 0.5 to 5.0 percent of fructus evodiae, 0.1 to 1.0 percent of lecithin, 10.0 to 30.0 percent of solvent, 0.5 to 2.0 percent of preservative and the balance of deionized water. The moisturizing and repairing composition can effectively remove free radicals (DPPH) and increase the water content of skin through the coordination effect of the components, has the functions of reducing water loss, repairing and protecting skin barriers, reducing skin wrinkles and the like, and can improve the problems of dry skin, dull yellow skin, desquamation, sensitive skin, fine wrinkles and the like.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a moisturizing and repairing composition, its preparation method, and its application. Background Technology

[0002] Skin is the largest organ in the human body. As we age, environmental factors, improper skincare, lifestyle habits, damage to the skin barrier, or the effects of diseases and medications can lead to dryness, flaking, and tightness. Long-term dehydration may accelerate skin aging or cause sensitivity problems. As people become more aware of skin care, the market demand for moisturizing and repairing cosmetics is also expanding.

[0003] There are many commercially available moisturizing and repairing products, but they still have many shortcomings. Most of these products are not compatible with various skin types and can only moisturize normal skin. They are not suitable for dry or sensitive skin, which may result in poor skincare effects or even allergic reactions.

[0004] Currently, there is a lack of products on the market that can provide both significant moisturizing and repairing effects and are suitable for all skin types. This is mainly because the raw materials are too simple, and they mainly use occlusive oils and polyol moisturizers to improve moisturization, dryness, and peeling. However, occlusive oils are not friendly to sensitive skin and can easily cause skin blockage, resulting in symptoms such as redness, swelling, and itching. The amount of polyols added is too high, which has strong hygroscopicity and is irritating. Moreover, it cannot provide long-lasting protection and cannot solve multiple skin problems such as allergies and sensitive skin.

[0005] Therefore, this application proposes a moisturizing and repairing composition, its preparation method, and its application. Summary of the Invention

[0006] The purpose of this invention is to provide a moisturizing and repairing composition, its preparation method and application, which effectively removes free radicals (DPPH), increases skin moisture content, reduces moisture loss, enhances skin's water-locking ability, repairs and protects the skin barrier, and reduces skin wrinkles. It can improve problems such as dry skin, dullness, peeling, sensitive skin, and fine lines.

[0007] According to a first aspect of the present invention, a moisturizing and repairing composition is provided, the composition comprising the following components in weight percentages: 3.0-10.0% of European beech buds, 3.0-10.0% of Bidens trifoliata, 2.0-10.0% of Spirulina macrophylla, 2.0-10.0% of raspberry fruit, 0.5-5.0% of Aconitum carmichaelii seed, 0.5-5.0% of Evodia rutaecarpa fruit, 0.1-1.0% of lecithin, 10.0-30.0% of solvent, 0.5-2.0% of preservative, and the balance being deionized water.

[0008] Among them, the buds of European beech are from Hunan, and the extracted part is the buds; the three-leaved begonia are from Guangdong, and the extracted part is the whole plant; the giant spirulina are from Shandong, and the extracted part is the whole plant; the raspberry fruit is from Hubei, and the extracted part is the fruit; the cowpea seeds are from Yunnan, and the extracted part is the seeds; and the fruit of Evodia rutaecarpa is from Yunnan, and the extracted part is the fruit.

[0009] According to an embodiment of the present invention, the lecithin is one or more of lecithin, phosphatidylcholine and soybean lecithin.

[0010] According to an embodiment of the present invention: the solvent is one or more combinations of 1,3-butanediol, glycerol, 2,3-butanediol, isopentyl glycol, isosorbide dimethyl ether, ethoxydiethylene glycol and bis-diethoxydiethylene cyclohexane 1,4-dicarboxylic acid ester.

[0011] According to an embodiment of the present invention: the preservative is one or more combinations of decanediol, p-hydroxyacetophenone, phenoxyethanol, ethylhexylglycerin, 1,2-hexanediol, 1,2-pentanediol, octyl glycol and glyceryl caprylate.

[0012] According to a second aspect of the present invention, a method for preparing a moisturizing and repairing composition is provided, comprising the following steps:

[0013] Step 1: Clean the European beech buds, Bidens trifoliata, Spirulina macrophylla, raspberry fruit, aconite seed, cowpea seed, and Evodia fruit. Place them in a 50-55℃ forced-air constant temperature oven for 12-15 hours. Weigh them according to the required amount, then grind them to 80-250nm using a swing-type ultrafine grinder and mix them together for later use.

[0014] Step 2: Mix the mixture from Step 1 with 95% ethanol at a mass ratio of 1:10-30 until homogeneous. Then add 20-50 times the weight of deionized water and soak for 5-10 minutes. Add the mixture to an ultrasonic extractor and extract for 30-45 minutes. After extraction, cool to room temperature and filter into a storage tank to obtain Mixture I.

[0015] Step 3: Add deionized water to the filtered residue mixture at a weight ratio of 1:30-50, then add 0.5-1.0% of the compound enzyme by weight of the residue mixture and mix well. Adjust the pH to 7.0-8.0 with tromethorphan, place in a 30-40℃ water bath and extract for 180-200 minutes. Then place the mixture in a 60-65℃ water bath for 8-15 minutes, then homogenize it using a high-pressure micro-jet homogenizer. After cooling to room temperature, filter it into a storage tank to obtain mixture II.

[0016] Step 4: Mix the filtered residue with the solvent evenly, then homogenize it using a high-pressure micro-jet homogenizer, and then filter it into a storage tank to form mixture III.

[0017] Step 5: Combine mixtures I, II, and III, stir at 35-45 rpm, weigh out the preservative and lecithin, add them to the container, stir for 20-30 minutes, homogenize three times using a high-pressure microfluidic homogenizer at 30,000-35,000 psi, and then centrifuge at 20,000-25,000 rpm for 30 minutes. Take the clear liquid from the previous step; this is the moisturizing and repairing composition.

[0018] According to an embodiment of the present invention: the composite enzyme is composed of cellulase, amylase, pectinase, β-glucanase, and protease in a mass ratio of 2:1:1:1:1.

[0019] According to an embodiment of the present invention: in step 2, the stirring speed of the ultrasonic extractor is 25-35 r / min, the working frequency is 20-25 kHz, and the temperature is 55-60℃.

[0020] According to an embodiment of the present invention: In step 3, the homogenization by the high-pressure microjet homogenizer includes: first homogenizing once with a high-pressure microjet homogenizer at a working pressure of 5000-6000psi, and then adjusting the working pressure to 25000-30000psi for two cycles of homogenization.

[0021] According to an embodiment of the present invention: In step 4, the homogenization by the high-pressure microjet homogenizer includes: first homogenizing once with a high-pressure microjet homogenizer with a working pressure of 10000-15000psi, and then adjusting the working pressure to 25000-30000psi for two cycles of homogenization.

[0022] According to a third aspect of the present invention, an application of the above-described moisturizing and repairing composition is provided, wherein the moisturizing and repairing composition is mixed with one of commonly used toners, serums, creams, eye creams, and lotions to obtain a skin care product with moisturizing and repairing effects, wherein the amount of the moisturizing and repairing composition added is 1 to 10% of the weight of the skin care product.

[0023] Compared with the prior art, the beneficial effects of the present invention are:

[0024] 1. The moisturizing and repairing composition of the present invention can effectively remove free radicals (DPPH) and increase skin moisture content through the synergistic effect of its components. It has the functions of reducing moisture loss, repairing and protecting the skin barrier, and reducing skin wrinkles. It can improve problems such as dry skin, dullness, peeling, sensitive skin, and fine lines.

[0025] 2. The moisturizing and repairing composition provided by this invention has a synergistic effect, which can promptly remove free radicals from the skin, inhibit lipid peroxidation of erythrocytes, effectively improve dull skin caused by oxidative damage, brighten skin tone, and reduce skin sagging. It also soothes sensitive skin, repairs skin inflammation and allergy symptoms, strengthens the damaged skin barrier, and resists external environmental damage to the skin.

[0026] 3. The moisturizing and repairing composition provided by this invention has a synergistic effect, which can significantly increase skin moisture, improve dry skin, peeling, and sensitive skin, enhance the dryness moisturizing and water-locking function, reduce allergic reactions caused by inflammation, improve skin allergies caused by seasonality and environment, and enhance the skin barrier function.

[0027] 4. The moisturizing and repairing composition provided by this invention has a synergistic effect, which can promote the expression of HAS-2 enzyme in the dermis, thereby promoting the synthesis of macromolecular hyaluronic acid, improving skin plumpness, helping to improve dullness, enhance skin radiance, and reduce wrinkles.

[0028] 5. The moisturizing and repairing composition provided by this invention has a synergistic effect, improves skin hydration, maintains skin elasticity, reduces damage to the skin from external pressure, increases skin moisture, relieves dry, rough, and sensitive skin, maintains moisture in the skin, and increases the skin repair barrier.

[0029] 6. The moisturizing and repairing composition provided by the present invention can exist stably in various aqueous emulsion formulations. It can play a good moisturizing and repairing role in various different formulations such as toner, facial mask liquid, lotion, face cream, and hair conditioner. The efficacy remains stable, and it is safe and non-irritating. It can be used to prepare a variety of topical preparations with moisturizing and soothing repair effects. Detailed Implementation

[0030] The technical solution of the present invention will be further described in detail below with reference to specific embodiments, but this does not constitute any limitation on the present invention. Any limited modifications made by anyone within the scope of protection of the present invention are still within the scope of protection of the present invention. It should be noted that, unless otherwise specified, the raw materials used in the technical solution provided by the present invention are all purchased from the market through conventional means.

[0031] Example 1

[0032] A moisturizing and repairing composition comprising the following components in weight percentage: 3.5% European beech buds, 6.0% Bidens trifoliata, 5.0% Spirulina macrophylla, 5.0% raspberry fruit, 0.5% Aconitum carmichaelii seed, 0.5% Evodia rutaecarpa fruit, 0.5% lecithin, 20.0% solvent, 1.0% preservative, and the balance being deionized water.

[0033] The lecithin mentioned is lecithin.

[0034] The solvent is a mixture of 1,3-butanediol and isosorbide dimethyl ether in a mass ratio of 10:1.

[0035] The preservative is a compound of p-hydroxyacetophenone, ethylhexylglycerin, and 1,2-hexanediol in a mass ratio of 1:0.1:1.

[0036] Example 2

[0037] A moisturizing and repairing composition comprising the following components in weight percentage: 5.0% European beech buds, 4.5% Bidens trifoliata, 4.5% Spirulina macrophylla, 4.5% raspberry fruit, 1.0% cowpea seed, 0.5% Evodia rutaecarpa fruit, 0.35% lecithin, 30.0% solvent, 0.5% preservative, and the balance being deionized water.

[0038] The lecithin is phosphatidylcholine.

[0039] The solvent is a mixture of glycerol and ethoxydiethylene glycol in a mass ratio of 10:1.

[0040] The preservative is a compound of decanediol, phenoxyethanol, ethylhexylglycerin, and caprylyl glycol in a mass ratio of 0.1:1:0.1:0.1.

[0041] Example 3

[0042] A moisturizing and repairing composition comprising the following components in weight percentage: 9.5% European beech buds, 3.0% Bidens trifoliata, 2.5% Spirulina macrophylla, 2.5% raspberry fruit, 2.5% cowpea seed with Aconitum carmichaelii leaf, 0.5% Evodia rutaecarpa fruit, 0.6% lecithin, 22.0% solvent, 2.0% preservative, and the balance being deionized water.

[0043] The lecithin is a compound of phosphatidylcholine and soybean lecithin in a mass ratio of 1:1.

[0044] The solvent is a mixture of isopentyl glycol and bis-diethoxydiethylene glycol cyclohexane 1,4-dicarboxylic acid ester in a mass ratio of 1:0.2.

[0045] The preservative is a compound of p-hydroxyacetophenone, 1,2-pentanediol, and ethylhexylglycerin in a mass ratio of 1:2:0.2.

[0046] Example 4

[0047] A moisturizing and repairing composition comprising the following components in weight percentage: 6.0% European beech buds, 4.5% Bidens trifoliata, 6.0% Spirulina macrophylla, 2.0% raspberry fruit, 1.5% cowpea seed, 0.5% Evodia rutaecarpa fruit, 0.2% lecithin, 10.0% solvent, 1.2% preservative, and the balance being deionized water.

[0048] The lecithin mentioned is soybean lecithin.

[0049] The solvent is a mixture of 2,3-butanediol, isopentanediol, and ethoxydiethylene glycol in a mass ratio of 1:1:0.2.

[0050] The preservative is a compound of decanediol, 1,2-hexanediol, and glyceryl caprylate in a mass ratio of 0.2:1:0.2.

[0051] Example 5

[0052] A moisturizing and repairing composition comprising the following components in weight percentage: 3.5% European beech buds, 5.5% Bidens trifoliata, 3.5% Spirulina macrophylla, 2.5% raspberry fruit, 5.0% cowpea seed, 0.5% Evodia rutaecarpa fruit, 0.75% lecithin, 30.0% solvent, 1.5% preservative, and the balance being deionized water.

[0053] The lecithin is a compound of lecithin and soybean lecithin used in a 1:1 mass ratio.

[0054] The solvent is a mixture of 1,3-butanediol and isopentanediol in a mass ratio of 1:1.

[0055] The preservative is a compound of p-hydroxyacetophenone, caprylyl glycol, and glyceryl caprylate in a mass ratio of 1:0.1:0.2.

[0056] Preparation Example

[0057] A method for preparing a moisturizing and repairing composition, comprising the following steps:

[0058] Step 1: Clean the European beech buds, Bidens trifoliata, Spirulina macrophylla, raspberry fruit, aconite seed, cowpea seed, and Evodia fruit. Place them in a 54℃ forced-air constant temperature oven for 14 hours. Weigh them according to the required amount, then grind them to 150nm using a swing-type ultrafine grinder and mix them together for later use.

[0059] Step 2: Mix the mixture from Step 1 with 95% ethanol at a mass ratio of 1:15 until homogeneous. Then add 45 times the weight of deionized water and soak for 9 minutes. Add the mixture to an ultrasonic extractor with a stirring speed of 28 r / min, a working frequency of 22 kHz, and a temperature of 58°C. Extract for 38 minutes. After extraction, cool to room temperature and filter into a storage tank to obtain Mixture I.

[0060] Step 3: Add deionized water to the filtered residue mixture at a weight ratio of 1:45, then add 0.8% of the weight of the compound enzyme (composed of cellulase, amylase, pectinase, β-glucanase, and protease in a mass ratio of 2:1:1:1:1) and mix well. Adjust the pH to 7.6 with tromethamine, place in a 37°C water bath and extract for 190 minutes. Then place the mixture in a 64°C water bath for 13 minutes. First, homogenize once with a high-pressure microfluidic homogenizer at a working pressure of 5800 psi, then adjust the working pressure to 29000 psi and homogenize twice. Finally, cool to room temperature and filter into a storage tank to obtain mixture II.

[0061] Step 4: Mix the filtered residue with the solvent evenly. First, homogenize it once with a high-pressure microjet homogenizer with a working pressure of 12500psi. Then, adjust the working pressure to 29000psi and homogenize it twice. Finally, filter it into the storage tank to form mixture III.

[0062] Step 5: Combine mixtures I, II, and III, stir at 43 rpm, weigh out the preservative and lecithin, add them to the container, stir for 26 minutes, homogenize three times using a high-pressure microfluidic homogenizer at 34000 psi, and then centrifuge at 20000 rpm for 30 minutes. Take the clear liquid from the previous step; this is the moisturizing and repairing composition.

[0063] The technical standards for the above-prepared moisturizing and repairing liquid composition are as follows:

[0064] Appearance: Colorless to light brownish-yellow transparent liquid;

[0065] pH: 5.0-7.0;

[0066] Stability (cold resistance): After being kept in a refrigerator at (-8±2)℃ for 30 days, there was no significant difference from the pre-test level after being restored to room temperature;

[0067] Stability (heat resistance): After being kept in a constant temperature chamber at (45±1)℃ for 30 days, there was no significant difference from the pre-test level after returning to room temperature;

[0068] Total bacterial count: <10 CFU / g;

[0069] Molds and yeasts: <10 CFU / g;

[0070] Thermoresistant coliforms: Not detectable;

[0071] Staphylococcus aureus: Not detectable;

[0072] Pseudomonas aeruginosa: Not detectable;

[0073] Heavy metals (lead ≤10mg / kg, mercury ≤1mg / kg, arsenic ≤2mg / kg, cadmium ≤5mg / kg, dioxane ≤30mg / kg, diethylene glycol: not detectable).

[0074] Comparative Example 1

[0075] Compared with Example 1, European beech buds were replaced with Bidens trifoliata, while the content of other components and the process remained unchanged.

[0076] Comparative Example 2

[0077] Compared with Example 1, the European beech buds were replaced with Spirulina macrophylla, while the content of other components and the process remained unchanged.

[0078] Comparative Example 3

[0079] Compared with Example 1, European beech was replaced with raspberry fruit, while the content of other components and the process remained unchanged.

[0080] Comparative Example 4

[0081] Compared with Example 1, the content of other components and the process of using *Aconitum carmichaelii* leaf cowpea for European beech remained unchanged.

[0082] Comparative Example 5

[0083] Compared with Example 1, European beech was replaced with Evodia fruit, while the content of other components and the process remained unchanged.

[0084] Experimental Example

[0085] To demonstrate the key technical points of the technical solution provided in this application, experimental data of embodiments 1-5 and comparative examples 1-5 provided in this application are given below;

[0086] 1. In vitro test—DPPH free radical scavenging test

[0087] 1.1 Principle: 1,1-Diphenyl-2-trinitrophenylhydrazine (DPPH) is a stable, long-lived free radical with a maximum absorption peak at 517 nm in the visible light region. In ethanol solution, each DPPH molecule generates a stable nitrogen-containing free radical with a typical purple color. When it reacts with a free radical scavenger that donates one electron, a colorless product is formed, causing the typical purple color of the solution to lighten. The degree of fading is stoichiometrically related to the number of paired electrons. Therefore, spectrophotometry can be used for quantitative analysis to detect free radical scavenging and evaluate the sample's ability to scavenge free radicals. If the extract can scavenge it, it indicates that the extract has the effect of reducing the effective concentration of hydroxyl radicals, alkyl radicals, or oxidative free radicals and interrupting lipid peroxidation chain reactions. Its ability is expressed as a scavenging rate; the higher the scavenging rate, the stronger its antioxidant capacity for scavenging free radicals.

[0088] 1.1.1 Reagent Preparation

[0089] Test solution: Dilute the moisturizing and repairing composition with deionized water to a mass percentage concentration of 1.0%.

[0090] DPPH ethanol solution: Dilute 10 mg DPPH with anhydrous ethanol and bring the volume to 1000 mL in a volumetric flask to prepare a DPPH concentration of 10 mg / L. Store at 0–5 °C protected from light. Prepare fresh and use immediately. Effective within 6 hours.

[0091] 1.1.2 Experimental Methods

[0092] Take 0.5 mL of the test solution and mix it with 2.5 mL of 10 mg / L DPPH ethanol solution until the total volume is 3 mL. The resulting solution is the sample group, and the absorbance is recorded as A1.

[0093] Take 0.5 mL of anhydrous ethanol and 2.5 mL of 10 mg / L DPPH ethanol solution and mix well to a total volume of 3 mL. The resulting solution is the blank control group, and the absorbance is recorded as A2.

[0094] Take 2.5 mL of anhydrous ethanol and mix it with 0.5 mL of the test solution to a total volume of 3 mL. The resulting solution is the negative control group. The absorbance is recorded as A3.

[0095] After reacting for 30 minutes, take 1.0 ml and measure the absorbance values ​​of A1, A2, and A3 at 517 nm.

[0096] 1.1.3 The reaction system was prepared according to the amount of each reagent added in Table 1, and the absorbance was measured at a wavelength of 517 nm.

[0097] Table 1 Reaction system (mL)

[0098] Add reagents Test liquid Anhydrous ethanol DPPH solution Example 1 0.5 0 2.5 Example 2 0.5 0 2.5 Example 3 0.5 0 2.5 Example 4 0.5 0 2.5 Example 5 0.5 0 2.5 Comparative Example 1 0.5 0 2.5 Comparative Example 2 0.5 0 2.5 Comparative Example 3 0.5 0 2.5 Comparative Example 4 0.5 0 2.5 Comparative Example 5 0.5 0 2.5 <![CDATA[A3]]> 0.5 2.5 0 <![CDATA[A2]]> 0.5 0 2.5

[0099] 1.1.4 Calculation formula:

[0100] The formula for calculating the clearance rate (CR) is as follows:

[0101]

[0102] In the formula:

[0103] A1 is the absorbance of the sample, that is, the absorbance of the solution after the test solution reacts with DPPH;

[0104] A2 represents the absorbance of the blank control group, i.e., the absorbance of the solution after the test solution reacts with anhydrous ethanol.

[0105] A3 represents the absorbance of the negative control group, i.e., the absorbance of the solution after the reaction of anhydrous ethanol with DPPH.

[0106] 1.1.5 Test Results

[0107] The experimental data results are shown in Table 2.

[0108] Table 2 Data Results

[0109] Sample Name Clearance rate (%) Example 1 59±2.59 Example 2 61±2.62 Example 3 67±2.87 Example 4 63±2.78 Example 5 62±2.75 Comparative Example 1 27±0.82 Comparative Example 2 23±0.80 Comparative Example 3 29±0.85 Comparative Example 4 25±0.82 Comparative Example 5 23±0.81

[0110] 1.1.6 Conclusion:

[0111] The experimental results show that Examples 1-5 have outstanding DPPH free radical scavenging ability, with a DPPH free radical scavenging rate of over 59%. However, Comparative Examples 1-5, after using various ingredient substitutions, showed a significant decrease in DPPH free radical scavenging rate. This demonstrates that the moisturizing and repairing composition provided by this invention has a strong ability to scavenge DPPH free radicals, effectively removing free radicals from the skin, inhibiting lipid peroxidation of erythrocytes, and significantly improving dull skin caused by oxidative damage, brightening skin tone, and reducing skin sagging.

[0112] 1.2 Cosmetic Moisturizing Efficacy Test - Moisturizing Weight Method:

[0113] 1.2.1 Principle: Based on the different forces that different moisturizer molecules in cosmetics exert on water molecules, their ability to absorb and retain water also varies slightly. The moisturizing efficacy of a moisturizer can be characterized by testing its moisturizing rate using a laboratory method (gravimetric method).

[0114] 1.2.2 Instruments and Materials:

[0115] Instruments: constant temperature and humidity chamber, analytical balance.

[0116] Test materials: test substance, water, 10% glycerol solution.

[0117] 1.2.3 Test substance: The original test sample;

[0118] 1.2.4 Blank control: Water;

[0119] 1.2.5 Standard reference: 10% glycerol solution;

[0120] 1.2.6 Test environment conditions: The test environment temperature is 20℃±1℃, the humidity is 45±2RH%, and real-time dynamic monitoring is carried out.

[0121] 1.2.7 Test Method:

[0122] Accurately weigh the sample and place it in a constant temperature and humidity chamber. Weigh the sample at 2, 4, 12, and 24 hours; record the data and calculate the moisture retention rate. Select a reagent with known moisturizing ability as a standard reference.

[0123] 1.2.8 Calculation of test results:

[0124] According to the experimental design, the Mt, Yt, and Ht values ​​were measured at each time point, and the moisture retention rate was calculated using the formula:

[0125] Moisture retention rate Psample = (Mt / M0) × 100%;

[0126] Moisture retention rate Pempt = (Yt / Y0) × 100%;

[0127] Moisture retention rate P_standard = (Ht / H0) × 100%;

[0128] Relative moisturizing rate % = [P sample - P blank] / [P standard - P blank] × 100%;

[0129] The relative moisture content of the test sample, blank control, and standard reference was calculated for each time period.

[0130] 1.2.9 Evaluation Criteria:

[0131] Based on the calculation of the relative moisture retention rate of the samples after 24 hours of continuous placement, the data can be divided into four levels: good, high, medium, and low moisturizing effect.

[0132] A relative moisturizing rate of % below 0% to 50% indicates low moisturizing performance.

[0133] A relative moisturizing rate of 50% to 100% indicates moderate moisturizing performance; a relative moisturizing rate of 100% to 150% indicates high moisturizing performance; and a relative moisturizing rate of 150% or higher indicates good moisturizing performance.

[0134] 1.2.10 Test Results:

[0135] Table 3 Relative Moisturizing Rate (%)

[0136]

[0137] 1.2.11 Conclusion:

[0138] Based on the change in relative moisture content of the test samples after 24 hours of continuous placement, the relative moisture content of the test samples in Examples 1-5 remained between 168.47% and 171.38% within 24 hours, while the relative moisture content of the test samples in Comparative Examples 1-5 remained between 96.05% and 98.13% within 24 hours. Therefore, the moisturizing performance is moderate, indicating that Examples 1-5 show that the samples have good moisturizing performance.

[0139] 2. To demonstrate the advantages of the present invention, the moisturizing and repairing compositions of Examples 1-5 and Comparative Examples 1-5 were added at 1.0% to the following moisturizing and repairing cream formulations; the moisturizing and repairing cream formulations are shown in Table 4 below:

[0140] Table 4 Moisturizing Repair Cream

[0141]

[0142] 2.1 Human Testing—Skin Moisture Content Test:

[0143] 2.1.1 Test objective: To evaluate the skin moisture content of the moisturizing and repairing creams of Examples 1-5 and Comparative Examples 1-5 before use and 1 hour, 2 hours and 4 hours after product use through a 4-hour human body moisturizing test. The higher the skin moisture content value, the higher the skin moisture content.

[0144] 2.1.2 Test Requirements:

[0145] Number of volunteers: 60 (40 women; 20 men, aged 35-55). They must be in good health, have no skin problems, be neither pregnant nor breastfeeding, and have no withdrawals from the test. No drugs or cosmetics will be used during the test.

[0146] Test area: outer side of the calf;

[0147] Test duration: 4 hours;

[0148] 2.1.3 Testing Instruments:

[0149] Corneometer (CM 825, Courage and Khazaka, Germany) for measuring skin moisture content;

[0150] 2.1.4 Test Procedure:

[0151] 1) Control the indoor temperature at 20-22℃ and the humidity at 40-60%.

[0152] 2) Divide 60 people into 11 groups of 5 people each. One group used a moisturizing repair cream without moisturizing ingredients (as a blank group).

[0153] 3) Test the test area on the skin before use, record the data as 0h and mark it.

[0154] 4) Weigh the test sample and apply it to the test area marked on the subject at a dosage of (2.0±0.1) mg / cm2. Massage for 30 seconds until absorbed, and then follow up.

[0155] 5) Repeat tests were conducted at 1 hour, 2 hours, and 4 hours after absorption. The untreated area was used as a blank for testing.

[0156] 2.1.5 Formula for calculating test results:

[0157] Initial value: refers to the base value when no product is used.

[0158] Where x = individual parameter measurement value, and n = number of valid data.

[0159] The difference from the initial value = x T使用后 -x T使用前 .

[0160]

[0161] 2.1.6 Test Results:

[0162] 2.1.6.1 The skin moisture content test results before product use and 1 hour, 2 hours and 4 hours after product use are shown in Table 5.

[0163] Table 5. Average skin moisture content and statistical results before product use, and 1 hour, 2 hours, and 4 hours after product use (n=50)

[0164]

[0165] 2.1.6.2 Conclusion:

[0166] The results in Table 5 show that, compared with before use, Examples 1-5 increased skin moisture content by more than 21.11% after 4 hours of use, while Comparative Examples 1-5 increased skin moisture content by less than 9.91% after 4 hours of use. Compared with the blank group after 4 hours of use, the skin moisture content increased significantly. This indicates that the components of the moisturizing and repairing compositions of Examples 1-5 of the present invention have a synergistic effect, with significant skin moisturizing and soothing ability. They can effectively improve dry skin and flaking problems, repair damaged skin barriers, make skin moist and smooth, and achieve long-lasting moisturizing and water-locking effects.

[0167] 3. Human body testing - Skin barrier function test:

[0168] 3.1 Principle: Skin moisture loss volume (TEWL) is an important parameter for assessing the function of the skin's moisture barrier and can be used to evaluate the strength of the skin barrier function. The amount of TEWL loss was used to evaluate the skin barrier repair function of Examples 1-5 and Comparative Examples 1-5; the smaller the measured value, the less skin moisture loss. In the repair test, a smaller TEWL indicates improved skin barrier function. The unit of TEWL is g / hm². 2 .

[0169] 3.2 Test Requirements:

[0170] 3.2.1 Sixty volunteers who participated in the skin moisture content test completed the test simultaneously, with one group using a moisturizing and repairing cream that did not contain a moisturizing and repairing composition.

[0171] 3.2.1 Testing Instruments:

[0172] Skin moisture loss test probe (Tewameter TM300, Courage & Khazaka, Germany).

[0173] 3.3 Formula for calculating test results:

[0174] Where x = individual parameter measurement value, and n = number of valid data;

[0175]

[0176] Table 6. Mean values ​​and statistical results of transdermal water loss (TEWL) before use and after 1, 2 and 4 hours of use (n=50).

[0177] Table 6. Average transdermal water loss (TEWL) and statistical results before use and after 1, 2, and 4 hours of use (n = 50)

[0178]

[0179]

[0180] 3.5 Conclusion:

[0181] The results in Table 6 show that, compared with the control group, Examples 1-5 significantly reduced TEWL loss, with a reduction of more than 10.67% in transepidermal water loss after 4 hours of use. In contrast, Comparative Examples 1-5 reduced transepidermal water loss by less than 4.22% after 4 hours of use. Compared with the control group, Examples 1-5 have stronger moisturizing ability, indicating that Examples 1-5 have lower TEWL loss, which means they repair the skin barrier function better. This can help promote the repair of the stratum corneum, improve skin problems such as dryness, roughness, sensitivity, and flaking, lock in moisture, and reduce wrinkles.

[0182] In summary, the moisturizing and repairing composition of the present invention, through the synergistic effect of its components, can effectively eliminate free radicals (DPPH), increase skin moisture content, reduce moisture loss, repair the skin barrier, and reduce skin wrinkles. It can improve problems such as dry skin, dullness, peeling, sensitive skin, and fine lines.

[0183] Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

Claims

1. A moisturizing and repairing composition, characterized in that: The moisturizing and repairing composition comprises the following components in weight percentage: 3.0-10.0% European beech buds, 3.0-10.0% Bidens trifoliata, 2.0-10.0% Spirulina macrophylla, 2.0-10.0% raspberry fruit, 0.5-5.0% Aconitum carmichaelii seed, 0.5-5.0% Evodia rutaecarpa fruit, 0.1-1.0% lecithin, 10.0-30.0% solvent, 0.5-2.0% preservative, and the balance being deionized water.

2. The moisturizing and repairing composition according to claim 1, characterized in that, The lecithin is one or more combinations of lecithin, phosphatidylcholine, and soybean lecithin.

3. The moisturizing and repairing composition according to claim 1, characterized in that, The solvent is one or more combinations of 1,3-butanediol, glycerol, 2,3-butanediol, isopentylene glycol, isosorbide dimethyl ether, ethoxydiethylene glycol, and bis-diethoxydiethylene cyclohexane 1,4-dicarboxylic acid ester.

4. The moisturizing and repairing composition according to claim 1, characterized in that, The preservative is one or more combinations of decanediol, p-hydroxyacetophenone, phenoxyethanol, ethylhexylglycerin, 1,2-hexanediol, 1,2-pentanediol, octyl glycol, and glyceryl caprylate.

5. A method for preparing a moisturizing and repairing composition, used to prepare the moisturizing and repairing composition according to any one of claims 1-4, characterized in that, Includes the following steps: Step 1: Clean the European beech buds, Bidens trifoliata, Spirulina macrophylla, raspberry fruit, aconite seed, cowpea seed, and Evodia fruit. Place them in a 50-55℃ forced-air constant temperature oven for 12-15 hours. Weigh them according to the required amount, then grind them to 80-250nm using a swing-type ultrafine grinder and mix them together for later use. Step 2: Mix the mixture from Step 1 with 95% ethanol at a mass ratio of 1:10-30 until homogeneous. Then add 20-50 times the weight of deionized water and soak for 5-10 minutes. Add the mixture to an ultrasonic extractor and extract for 30-45 minutes. After extraction, cool to room temperature and filter into a storage tank to obtain Mixture I. Step 3: Add deionized water to the filtered residue mixture at a weight ratio of 1:30-50, then add 0.5-1.0% of the compound enzyme by weight of the residue mixture and mix well. Adjust the pH to 7.0-8.0 with tromethorphan, place in a 30-40℃ water bath and extract for 180-200 minutes. Then place the mixture in a 60-65℃ water bath for 8-15 minutes, then homogenize it using a high-pressure micro-jet homogenizer. After cooling to room temperature, filter it into a storage tank to obtain mixture II. Step 4: Mix the filtered residue with the solvent evenly, then homogenize it using a high-pressure micro-jet homogenizer, and then filter it into a storage tank to form mixture III. Step 5: Combine mixtures I, II, and III, stir at 35-45 rpm, weigh out the preservative and lecithin, add them to the container, stir for 20-30 minutes, homogenize three times using a high-pressure microfluidic homogenizer at 30,000-35,000 psi, and then centrifuge at 20,000-25,000 rpm for 30 minutes. Take the clear liquid from the previous step; this is the moisturizing and repairing composition.

6. The method for preparing a moisturizing and repairing composition according to claim 5, characterized in that, The complex enzyme is composed of cellulase, amylase, pectinase, β-glucanase, and protease in a mass ratio of 2:1:1:1:

1.

7. The method for preparing a moisturizing and repairing composition according to claim 5, characterized in that, In step 2, the ultrasonic extractor has a stirring speed of 25-35 r / min, a working frequency of 20-25 kHz, and a temperature of 55-60℃.

8. The method for preparing a moisturizing and repairing composition according to claim 5, characterized in that, In step 3, the homogenization process using the high-pressure microjet homogenizer includes: first, homogenizing once with a high-pressure microjet homogenizer at a working pressure of 5000-6000psi, and then adjusting the working pressure to 25000-30000psi for two more cycles of homogenization.

9. The method for preparing a moisturizing and repairing composition according to claim 5, characterized in that, In step 4, the homogenization process using the high-pressure microjet homogenizer includes: first, homogenizing once with a high-pressure microjet homogenizer at a working pressure of 10,000-15,000 psi, and then adjusting the working pressure to 25,000-30,000 psi for two more cycles of homogenization.

10. The application of the moisturizing and repairing composition according to any one of claims 1-4, characterized in that, The moisturizing and repairing composition is mixed with one of commonly used toners, serums, creams, eye creams, and lotions to obtain a skin care product with moisturizing and repairing effects. The amount of the moisturizing and repairing composition added is 1 to 10% of the weight of the skin care product.

Images