Compositions for inhibiting propionibacterium acnes and methods for making same, acne cosmetic

Abstract

The application relates to the field of cosmetics, in particular to a composition for inhibiting propionibacterium acnes, a preparation method thereof and an acne-removing cosmetic. The composition for inhibiting propionibacterium acnes is composed of a solution of manglietia extract and a solution of paeonia extract; the mass ratio of the solution of manglietia extract and the solution of paeonia extract is (1-4):(4-1). The technical scheme provides a composition containing a solution of manglietia extract and a solution of paeonia extract, the composition can stably inhibit the growth of propionibacterium acnes, thereby achieving the effect of acne removal. The two components in the composition can produce a synergistic effect within the above-mentioned ratio range, thereby improving the effect of inhibiting propionibacterium acnes. Moreover, the two components in the composition are green and environmentally friendly, the acne-removing effect is stable, and the acne-removing components such as salicylic acid, azelaic acid, benzoyl peroxide and retinoids are green, natural, non-irritating and free of side effects.

Description

Technical Field

[0001] This application relates to the field of cosmetics, and more specifically, to a composition for inhibiting Propionibacterium acnes, a method for preparing the same, and acne-removing cosmetics. Background Technology

[0002] With the fast pace of life and increasing social pressure, acne has become a significant factor affecting the skin health of a wide range of people.

[0003] Currently, most acne treatment products on the market use chemical ingredients such as salicylic acid, azelaic acid, benzoyl peroxide, and retinoids to treat acne. However, these acne treatment ingredients have unstable effects, are highly irritating, and are prone to causing side effects. Summary of the Invention

[0004] The purpose of this application is to provide a composition for inhibiting Propionibacterium acnes, a method for preparing the same, and an acne-removing cosmetic.

[0005] In a first aspect, this application provides a composition for inhibiting Propionibacterium acnes, comprising the following components: Magnolia extract solution and Pioneerine solution; The mass ratio of the magnolia extract solution to the piocin solution is (1~4):(4~1).

[0006] The above technical solution provides a composition containing magnolia extract solution and piocinol solution, which can stably inhibit the growth of Propionibacterium acnes, thereby achieving an acne-removing effect. Within the aforementioned ratio range, the two components in this composition can produce a synergistic effect, enhancing the inhibition of Propionibacterium acnes. Furthermore, the two components in this composition are environmentally friendly, and compared to chemical acne-removing ingredients such as salicylic acid, azelaic acid, benzoyl peroxide, and retinoids, they offer stable acne-removing effects and are natural, non-irritating, and have no side effects.

[0007] In other embodiments of this application, the mass ratio of the above-mentioned magnolia extract solution and piocin solution is 1:1.

[0008] In other embodiments of this application, the concentration of the magnolia extract solution is 1% to 3% by mass percentage.

[0009] In other embodiments of this application, the mass ratio of the magnolia extract solution and the piocin solution is 1:1.

[0010] In other embodiments of this application, the concentration of the magnolia extract solution is 1% to 3% by mass percentage.

[0011] In other embodiments of this application, the concentration of the piodin solution is 0.5% to 1% by mass percentage.

[0012] In other embodiments of this application, the magnolia extract solution comprises: an alcoholic extract of the magnolia plant.

[0013] In other embodiments of this application, the magnolia extract solution comprises: an ethanol extract of the magnolia plant.

[0014] In other embodiments of this application, the mass ratio of the magnolia extract solution to the piocin solution is (1~3):(3~1).

[0015] Secondly, this application provides a method for preparing a composition that inhibits Propionibacterium acnes, comprising: Magnolia plant was extracted with alcohol, and the extract was collected to obtain Magnolia extract; the Magnolia extract was diluted to obtain Magnolia extract solution; Piocin is mixed with water to obtain a piocin solution; Mix the magnolia extract solution and the piocin solution at a mass ratio of (1~4):(4~1) until homogeneous.

[0016] In other embodiments of this application, diluting the magnolia extract into a magnolia extract solution includes diluting the magnolia extract to a concentration of 1% to 3%.

[0017] In other embodiments of this application, the step of mixing the piodin with water to obtain a piodin solution includes: diluting the piodin to a concentration of 0.5% to 1%.

[0018] Thirdly, this application provides an acne-removing cosmetic, comprising the composition for inhibiting Propionibacterium acnes provided in any of the first aspects; or the acne-removing cosmetic comprises the composition for inhibiting Propionibacterium acnes prepared in any of the second aspects. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions in the embodiments of this application will be clearly and completely described below. Obviously, the described embodiments are some embodiments of this application, but not all embodiments.

[0020] Therefore, the following detailed description of the embodiments of this application is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments of the application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

[0021] Research has shown that pimples, especially acne, are mainly caused by the overgrowth of Propionibacterium acnes; therefore, inhibiting the growth of Propionibacterium acnes can achieve the effect of removing acne.

[0022] Based on this, embodiments of this application provide a composition for inhibiting Propionibacterium acnes, comprising the following components: Magnolia extract solution and Pioneerine solution; The mass ratio of the magnolia extract solution to the piocin solution is (1~4):(4~1).

[0023] The above technical solution provides a composition containing a magnolia extract solution and a piodin (quaternary ammonium salt-73) extract solution. This composition can stably inhibit the growth of Propionibacterium acnes, thereby achieving an acne-removing effect. Within the aforementioned ratio range, the two components in this composition can produce a synergistic effect, enhancing the inhibition of Propionibacterium acnes. Furthermore, the two components in this composition are environmentally friendly. Compared to chemical acne-removing ingredients such as salicylic acid, azelaic acid, benzoyl peroxide, and retinoids, this composition offers stable acne-removing effects and is natural, non-irritating, and has no side effects.

[0024] Further optionally, and exemplary, in some embodiments of this application, the mass ratio of the magnolia extract solution and the piodin solution is 1:4, 1:3.5, 1:3, 1:2.5, 1:2, 1:1.5, 1:1, 1.5:1, 2:1, 2.5:1, 3:1, 3.5:1, 4:1, or any range between the aforementioned two values.

[0025] Further, optionally, in some embodiments of this application, the mass ratio of the magnolia extract solution and the piocin solution is 1:1.

[0026] Furthermore, in some embodiments of this application, the concentration of the magnolia extract solution is 1% to 3% by mass percentage.

[0027] For example, in some embodiments of this application, the concentration of the above-mentioned magnolia extract solution, by mass percentage, is 1%, 1.5%, 1.8%, 2%, 2.1%, 2.5%, 2.8%, 3%, or any two of the aforementioned values.

[0028] Furthermore, in some embodiments of this application, the concentration of the piodin solution is 0.5% to 1% by mass percentage.

[0029] For example, in some embodiments of this application, the concentration of the above-mentioned piodin solution, by mass percentage, is 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, or a range between any two of the aforementioned values.

[0030] Furthermore, in some embodiments of this application, the magnolia extract solution comprises: an alcoholic extract of the magnolia plant.

[0031] Furthermore, in some embodiments of this application, the magnolia extract includes: an ethanol extract of the magnolia plant.

[0032] Furthermore, in some embodiments of this application, the mass ratio of the magnolia extract solution to the piocin solution is (1~3):(3~1).

[0033] Some embodiments of this application provide a method for preparing a composition that inhibits Propionibacterium acnes, comprising: Magnolia plant was extracted with alcohol, and the extract was collected to obtain Magnolia extract; the Magnolia extract was diluted to obtain Magnolia extract solution; Piocin is mixed with water to obtain a piocin solution; Mix the magnolia extract solution and the piocin solution at a mass ratio of (1~4):(4~1) until homogeneous.

[0034] The above technical solution can effectively prepare a composition containing magnolia extract solution and piocin solution, which can stably inhibit the growth of Propionibacterium acnes, thereby achieving the effect of acne removal.

[0035] Further, optionally, in some embodiments of this application, the magnolia plant is extracted with alcohol, and the extract is obtained to obtain a magnolia extract, comprising: The magnolia plant was extracted with ethanol, and the extract was collected.

[0036] Alternatively, in some embodiments of this application, after extracting the magnolia plant with solvents such as ethanol, the effective components are further extracted through steps such as concentration and extraction.

[0037] Further optionally, in some embodiments of this application, pionin (quaternary ammonium salt-73) is a synthetically produced chemical substance obtained by pure chemical synthesis, which can be synthesized using conventional methods in the art.

[0038] Further, alternatively, in some embodiments of this application, the above-mentioned pionin (quaternary ammonium salt-73) can be obtained by purchasing commercially available products.

[0039] Further optionally, and exemplary, in some embodiments of this application, the magnolia extract solution and the piodin solution are mixed evenly in a mass ratio of 1:4, 1:3.5, 1:3, 1:2.5, 1:2, 1:1.5, 1:1, 1.5:1, 2:1, 2.5:1, 3:1, 3.5:1, 4:1 or any range between the aforementioned two values.

[0040] Furthermore, in some embodiments of this application, the magnolia extract is diluted into a magnolia extract solution, including diluting the magnolia extract to a concentration of 1% to 3%.

[0041] For example, in some embodiments of this application, diluting the magnolia extract into a magnolia extract solution includes diluting the magnolia extract to a concentration of 1%, 1.2%, 1.5%, 1.8%, 2%, 2.2%, 2.5%, 2.8%, 2.9%, 3%, or any two of the aforementioned values.

[0042] Furthermore, in some embodiments of this application, piocin is diluted to a concentration of 0.5% to 1%.

[0043] For example, in some embodiments of this application, diluting piocin to a concentration of 0.5% to 1% includes diluting piocin to a concentration of 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, or any two of the aforementioned values.

[0044] Some embodiments of this application provide an acne-removing cosmetic, including the composition for inhibiting Propionibacterium acnes provided in any of the foregoing embodiments; or the acne-removing cosmetic includes the composition for inhibiting Propionibacterium acnes prepared in any of the foregoing embodiments.

[0045] In the above technical solution, the acne-removing cosmetic can effectively improve the acne-removing effect of the cosmetic by adding the above-mentioned composition that inhibits Propionibacterium acnes; and the acne-removing active ingredients in the acne-removing cosmetic are green and natural, and compared with chemical acne-removing ingredients such as salicylic acid, azelaic acid, benzoyl peroxide, and retinoic acid, the acne-removing effect is stable, and it is green, natural, non-irritating, and has no side effects.

[0046] The features and performance of this application will be further described in detail below with reference to embodiments: Example 1 A composition for inhibiting Propionibacterium acnes is provided, the composition comprising the following components: Magnolia extract solution and Pioneerine solution; The mass ratio of the magnolia extract solution to the piocin solution is 1:1.

[0047] The composition was prepared according to the following steps: (1) Extract the magnolia plant with alcohol and obtain the extract to obtain magnolia extract; dilute the magnolia extract with water to a concentration of 3% by mass percentage; obtain the diluted magnolia extract solution. (2) Prepare a solution by mixing Pionin (quaternary ammonium salt-73) with water; the concentration of the Pionin solution is 1% by mass percentage. (3) Mix the magnolia extract solution obtained in step (1) and the pionemine solution obtained in step (2) at a mass ratio of 1:1.

[0048] Example 2 A composition for inhibiting Propionibacterium acnes is provided, the composition comprising the following components: Magnolia extract solution and Pioneerine solution; The mass ratio of the magnolia extract solution to the piocin solution is 2:1.

[0049] The composition was prepared according to the following steps: (1) Extract the magnolia plant with alcohol and obtain the extract to obtain magnolia extract; dilute the magnolia extract with water to a concentration of 3% by mass percentage; obtain the diluted magnolia extract solution. (2) Prepare a solution by mixing Pionin (quaternary ammonium salt-73) with water; the concentration of the Pionin solution is 1% by mass percentage. (3) Mix the magnolia extract solution obtained in step (1) and the piocin solution obtained in step (2) at a mass ratio of 2:1.

[0050] Example 3 A composition for inhibiting Propionibacterium acnes is provided, the composition comprising the following components: Magnolia extract solution and Pioneerine solution; The mass ratio of the magnolia extract solution to the piocin solution is 1:2.

[0051] The composition was prepared according to the following steps: (1) Extract the magnolia plant with alcohol and obtain the extract to obtain magnolia extract; dilute the magnolia extract with water to a concentration of 3% by mass percentage; obtain the diluted magnolia extract solution. (2) Prepare a solution by mixing Pionin (quaternary ammonium salt-73) with water; the concentration of the Pionin solution is 1% by mass percentage. (3) Mix the magnolia extract solution obtained in step (1) and the piocin solution obtained in step (2) at a mass ratio of 1:2.

[0052] Example 4 A composition for inhibiting Propionibacterium acnes is provided, the composition comprising the following components: Magnolia extract solution and Pioneerine solution; The mass ratio of the magnolia extract solution to the piocin solution is 1:3.

[0053] The composition was prepared according to the following steps: (1) Extract the magnolia plant with alcohol and obtain the extract to obtain magnolia extract; dilute the magnolia extract with water to a concentration of 3% by mass percentage; obtain the diluted magnolia extract solution. (2) Prepare a solution by mixing Pionin (quaternary ammonium salt-73) with water; the concentration of the Pionin solution is 1% by mass percentage. (3) Mix the magnolia extract solution obtained in step (1) and the piocin solution obtained in step (2) at a mass ratio of 1:3.

[0054] Example 5 A composition for inhibiting Propionibacterium acnes is provided, the composition comprising the following components: Magnolia extract solution and Pioneerine solution; The mass ratio of the magnolia extract solution to the piocin solution is 3:1.

[0055] The composition was prepared according to the following steps: (1) Extract the magnolia plant with alcohol and obtain the extract to obtain magnolia extract; dilute the magnolia extract with water to a concentration of 3% by mass percentage; obtain the diluted magnolia extract solution. (2) Prepare a solution by mixing Pionin (quaternary ammonium salt-73) with water; the concentration of the Pionin solution is 1% by mass percentage. (3) Mix the magnolia extract solution obtained in step (1) and the piocin solution obtained in step (2) at a mass ratio of 3:1.

[0056] Example 6 A composition for inhibiting Propionibacterium acnes is provided, the composition comprising the following components: Magnolia extract solution and Pioneerine solution; The mass ratio of the magnolia extract solution to the piocin solution is 1:4.

[0057] The composition was prepared according to the following steps: (1) Extract the magnolia plant with alcohol and obtain the extract to obtain magnolia extract; dilute the magnolia extract with water to a concentration of 3% by mass percentage; obtain the diluted magnolia extract solution. (2) Prepare a solution by mixing Pionin (quaternary ammonium salt-73) with water; the concentration of the Pionin solution is 1% by mass percentage. (3) Mix the magnolia extract solution obtained in step (1) and the piocin solution obtained in step (2) at a mass ratio of 1:4.

[0058] Example 7 A composition for inhibiting Propionibacterium acnes is provided, the composition comprising the following components: Magnolia extract solution and Pioneerine solution; The mass ratio of the magnolia extract solution to the piocin solution is 4:1.

[0059] The composition was prepared according to the following steps: (1) Extract the magnolia plant with alcohol and obtain the extract to obtain magnolia extract; dilute the magnolia extract with water to a concentration of 3% by mass percentage; obtain the diluted magnolia extract solution. (2) Prepare a solution by mixing Pionin (quaternary ammonium salt-73) with water; the concentration of the Pionin solution is 1% by mass percentage. (3) Mix the magnolia extract solution obtained in step (1) and the piocin solution obtained in step (2) at a mass ratio of 4:1.

[0060] Comparative Example 1 A magnolia extract solution is provided, prepared according to the following steps: Magnolia plant was extracted with alcohol to obtain Magnolia extract; water was then added to dilute the Magnolia extract to a concentration of 1% by mass to obtain a diluted Magnolia extract solution.

[0061] Comparative Example 2 A magnolia extract solution is provided, prepared according to the following steps: Magnolia plant was extracted with alcohol to obtain Magnolia extract; water was then added to dilute the Magnolia extract to a concentration of 2% by mass percentage to obtain a diluted Magnolia extract solution.

[0062] Comparative Example 3 A magnolia extract solution is provided, prepared according to the following steps: Magnolia plant was extracted with alcohol to obtain Magnolia extract; water was then added to dilute the Magnolia extract to a concentration of 3% by mass percentage to obtain a diluted Magnolia extract solution.

[0063] Comparative Example 4 A magnolia extract solution is provided, prepared according to the following steps: Magnolia plant was extracted with alcohol to obtain Magnolia extract; water was then added to dilute the Magnolia extract to a concentration of 3.5% by mass to obtain a diluted Magnolia extract solution.

[0064] Comparative Example 5 A piodin solution is provided, prepared according to the following steps: Prepare a solution by mixing piodin (quaternary ammonium salt-73) with water; the concentration of the piodin solution is 0.5% by mass percentage.

[0065] Comparative Example 6 A piodin solution is provided, prepared according to the following steps: Prepare a solution by mixing piodin (quaternary ammonium salt-73) with water; the concentration of the piodin solution is 0.8% by mass percentage.

[0066] Comparative Example 7 A piodin solution is provided, prepared according to the following steps: Prepare a solution by mixing piodin (quaternary ammonium salt-73) with water; the concentration of the piodin solution is 1% by mass percentage.

[0067] Comparative Example 8 A piodin solution is provided, prepared according to the following steps: Prepare a solution by mixing piodin (quaternary ammonium salt-73) with water; the concentration of the piodin solution is 1.5% by mass percentage.

[0068] The inhibitory performance of the samples prepared in each embodiment and comparative example on Propionibacterium acnes was tested.

[0069] Experimental Example 1 (1) Activate Propionibacterium acnes and prepare a bacterial suspension, and adjust its bacterial concentration to 1.0~2.0*10. 6 CFU / mL, take 100μL of the above bacterial suspension and inoculate it into the prepared enhanced Clostridium agar plate, and spread it evenly with a spreader; (2) Preparation of antibacterial tablets and control samples: Take sterile dry filter paper (5 mm in diameter), add 20 μL of the composition solution to each paper, place it in a sterile petri dish, open the lid and dry it in a constant temperature oven at 36°C for later use. The control sample paper is prepared with sterile distilled water. (3) Placement of antibacterial patches: Place 4 test patches from each example or comparative example, and 1 control patch on each contaminated plate, for a total of 5 patches. Use sterile forceps to place the patches on the plate surface, with the negative control patch placed in the center and the test patches placed around the perimeter. The center of each patch should be at least 25 mm apart, and the patch should be at least 15 mm away from the perimeter of the plate. Cover the plate and incubate at 36℃ for 18 hours to observe the results. Repeat the experiment 3 times. (4) Measure and record the diameter of the inhibition zone (including the patch). When measuring the inhibition zone, an inhibition zone with completely sterile growth should be selected.

[0070] The test results are shown in Tables 1-3.

[0071] Table 1

[0072] As can be seen from the test results in Table 1 above, the diameter of the inhibition zone in each embodiment is larger than that in the control sample; this demonstrates that the solutions in each embodiment of this application can effectively improve the antibacterial effect.

[0073] Furthermore, the results of the comparative tests (antibacterial test results of magnolia extract) for Examples 1-3 are shown in Table 2: Table 2

[0074] The test results above show that the diameter of the inhibition zone in each comparative example is smaller than that in each embodiment; this indicates that, compared with the single Magnolia extract, the components in each embodiment produce a synergistic effect, thus improving the antibacterial effect.

[0075] Furthermore, Comparative Examples 4-6 (results of antibacterial test of piocin solution) are shown in Table 3: Table 3

[0076] The test results above show that the diameter of the inhibition zone in each comparative example is smaller than that in each embodiment; this indicates that, compared with piocin alone, the components in each embodiment produce a synergistic effect, thus improving the antibacterial effect.

[0077] In summary, the components of the composition provided in this application have a synergistic effect, effectively enhancing the inhibition of Propionibacterium acnes.

[0078] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A composition for inhibiting Propionibacterium acnes, characterized by comprising, It consists of the following components: Magnolia extract solution and piocin solution; the magnolia extract is obtained by extracting the magnolia plant with alcohol and taking the extract. The mass ratio of the magnolia extract solution to the piocin solution is 1:1 or 1:2; the concentration of the magnolia extract solution is 1% to 3% by mass percentage; and the concentration of the piocin solution is 0.5% to 1% by mass percentage.

2. The composition for inhibiting Propionibacterium acnes according to claim 1, characterized in that, The magnolia extract includes: an ethanol extract of the magnolia plant.

3. The method of preparing a composition for inhibiting P. acnes according to any one of claims 1-2, wherein, include: Magnolia plant was extracted with alcohol, and the extract was collected to obtain Magnolia extract; The magnolia extract was diluted to prepare a magnolia extract solution; Piocin is mixed with water to obtain a piocin solution; Mix the magnolia extract solution and the piocin solution evenly.

4. An anti-acne cosmetic, characterized by, The composition comprising the Propionibacterium acnes inhibitory composition according to any one of claims 1-2; or the acne-removing cosmetic comprising the Propionibacterium acnes inhibitory composition prepared by the method for preparing the Propionibacterium acnes inhibitory composition according to claim 3.

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