Staphylococcus aureus and methicillin-resistant staphylococcus aureus detection primer group, kit and application thereof

Abstract

The invention discloses a primer group and a kit for detecting staphylococcus aureus and methicillin-resistant staphylococcus aureus and application of the primer group and the kit, and solves the problems that in the prior art, the detection sensitivity of the staphylococcus aureus (SA) and the methicillin-resistant staphylococcus aureus (MRSA) is low, false positive exists, and consumed time is long. The kit for detecting Staphylococcus aureus (SA) and methicillin-resistant Staphylococcus aureus (MRSA) provided by the invention adopts a method of combining isothermal amplification with immunochromatography, effectively solves the problem that a loop-mediated isothermal amplification fluorescent probe is not matched, has the detection limit sensitivity as low as 100 copies / mL, can complete detection within 35 minutes, and is visual in result and simple and convenient to interpret; the kit provided by the invention has the advantages of low single detection cost and low requirements on instruments and equipment, is suitable for primary medical units, can be used for home self-test, and is beneficial to early diagnosis of bacterial infection.

Images