一种鉴定柑橘硼毒害抗性的分子标记、引物及应用
By developing molecular markers and PCR detection primers for citrus boron toxicity resistance and designing 3-KAT promoter insertion sites, the problem of identifying citrus boron toxicity resistance was solved, enabling efficient and economical variety breeding.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- POMOLOGY RES INST FUJIAN ACAD OF AGRI SCI
- Filing Date
- 2025-10-10
- Publication Date
- 2026-07-17
AI Technical Summary
The lack of effective molecular markers for identifying boron toxicity resistance in citrus has limited the breeding of boron-resistant citrus varieties, resulting in widespread and difficult-to-control boron toxicity in citrus-producing areas.
A molecular marker and PCR detection primer for identifying boron resistance in citrus was developed. Primers BS-P2-F and BS-P2-R were designed using a specific insertion site on the 3-KAT promoter. The PCR products of 442 bp and 231 bp were identified by PCR amplification, distinguishing between boron-resistant and susceptible varieties.
This method enables high-throughput, low-cost identification of boron toxicity resistance in citrus, supports precision breeding, and improves the efficiency of breeding boron-resistant citrus varieties.
Smart Images
Figure CN120967056B_ABST