A SCAR marker for identifying the purity of seeds from two Allium species and its application

By using SCAR marker technology and specific primer set PCR amplification, onion and scallion seeds were distinguished using 1388bp and 760bp SCAR marker fragments, solving the problem of seed contamination and achieving efficient and accurate seed purity identification.

CN120967058BActive Publication Date: 2026-07-17HENAN KANGDA SEED TECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HENAN KANGDA SEED TECHNOLOGY CO LTD
Filing Date
2025-10-11
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing technologies cannot accurately distinguish between onion and scallion seeds, resulting in seed mixing. Traditional field identification methods are greatly affected by environmental and human factors, making it difficult to efficiently identify seed purity.

Method used

Using SCAR marker technology, specific primer sets were designed to amplify onion and scallion seeds by PCR. Seed purity was identified by agarose gel electrophoresis or first-generation sequencing. Onions and scallions were distinguished by specific SCAR marker fragments of 1388bp and 760bp.

Benefits of technology

It enables accurate identification of onion and scallion seeds, improves identification efficiency, reduces the influence of human and environmental factors, and can stably distinguish seeds of different varieties in batch experiments.

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Abstract

This invention belongs to the field of biotechnology, specifically relating to a SCAR marker for identifying the purity of seeds from two Allium species and its application. The SCAR marker-specific fragments for onion are 1388 bp and 760 bp in length, with nucleotide sequences shown in SEQ ID NO: 1 and SEQ ID NO: 2, respectively. The SCAR marker-specific fragment for leek is 760 bp in length, with nucleotide sequences shown in SEQ ID NO: 3, respectively. The SCAR markers identified in this invention exhibit high specificity and stability. Multiple biological replications on seeds from 10 varieties of onion and 10 varieties of leek, sourced from a wide range of sources, demonstrate that they can reliably distinguish between onion and leek seeds. This overcomes the shortcomings of traditional morphological identification methods, which are inaccurate and time-consuming, significantly improving the efficiency of identifying the purity of onion and leek seeds. It provides a rapid and feasible method for identifying the purity of onion and leek seeds in production.
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