A hyaluronic acid purity detection device and a detection method

Abstract

The application relates to a hyaluronic acid purity detection device and a matching detection method thereof, and belongs to the technical field of hyaluronic acid purity detection. The device takes a detection box body as a core carrier, is internally divided into a sealed detection inner cavity used for placing a test tube and a working inner cavity used for installing functional components, and is externally provided with a precision controller. The device comprises a precision control pen, a multi-channel valve and a main control, supports handheld quantitative sampling, realizes function switching of hyaluronic acid delivery, cleaning liquid flushing and high-pressure gas drying through the multi-channel valve, cooperates with symmetrically distributed cleaning holes and drying holes, and can effectively remove pipeline residues. Therefore, positioning control of sample delivery, cleaning and detection is realized, pipeline internal cleaning and component external cleaning are greatly improved, and the goals of low sampling error, less residual pollution and high detection efficiency are further realized.

Description

Technical Field

[0001] This invention relates to the technical field of hyaluronic acid purity detection, and particularly to a hyaluronic acid purity detection device and detection method. Background Technology

[0002] Hyaluronic acid, also known as hyaluronic acid, has good biocompatibility, moisturizing properties and viscoelasticity, and is widely used in the fields of medicine, cosmetics, and food.

[0003] In different applications, the purity of hyaluronic acid directly determines product performance and safety: pharmaceutical-grade hyaluronic acid requires strict control of impurities such as proteins, nucleic acids, and microorganisms, while cosmetic-grade hyaluronic acid requires restrictions on heavy metal content. Therefore, accurate and efficient purity testing is a core requirement in the production, processing, and quality inspection of hyaluronic acid.

[0004] For example, Chinese patent CN220490784U discloses a device for efficiently detecting the content of hyaluronic acid composition. This device can use an electric telescopic rod to drive a piston to squeeze the liquid in the storage box, so as to achieve precise quantitative addition of the liquid according to the needs. In combination with the rotation of the stirring rod, the solution to be tested is prepared, thereby improving the preparation efficiency and facilitating efficient detection of the content of hyaluronic acid composition.

[0005] However, the above-mentioned device still has some shortcomings in actual use:

[0006] 1. In the aforementioned prior art, the sampling method relies on manual pouring or pipetting to extract hyaluronic acid, which cannot ensure that the sample volume is consistent each time, resulting in poor repeatability of the test results. Furthermore, during sampling, the existing device cannot quantitatively extract and test the material from different depth regions of the hyaluronic acid to be sampled. In particular, when extracting hyaluronic acid from the middle region of a whole tube of hyaluronic acid, the existing device requires the use of a dropper for extraction, and the amount extracted cannot be controlled. When performing purity testing, this will affect the accuracy of the test results.

[0007] 2. The existing equipment mainly uses manual rinsing and natural air drying to clean the sampling pipelines. This can easily lead to the residue of hyaluronic acid samples in the tiny gaps on the inner wall of the pipeline, which can contaminate the new sample during the next sampling and cause deviations in the test results. Furthermore, natural air drying takes a long time and microorganisms can easily grow inside the pipeline, affecting the safety of the test.

[0008] Therefore, based on the above-stated viewpoints, there is still room for improvement in existing devices. Summary of the Invention

[0009] To address the aforementioned problems, this invention provides a hyaluronic acid purity detection device and method, employing the following technical solution:

[0010] In a first aspect, this application provides a hyaluronic acid purity testing device, including a stationary testing chamber, a testing cavity inside the testing chamber that opens to one side and communicates with the outside, a mounting bracket for loading test tubes that is slidably inserted into the testing cavity along its length, and a working cavity inside the testing chamber.

[0011] One side of the testing chamber is equipped with a precision controller for accurately drawing and quantifying hyaluronic acid; another side of the testing chamber has an inward recess for housing the precision controller.

[0012] Detectors connected via a cross control system are also installed along the inner wall of the detection chamber.

[0013] The precision controller includes a movable precision pen and a multi-channel valve mounted on the top of the precision pen. The multi-channel valve is also equipped with a control tube, one end of which is connected to a nozzle that delivers hyaluronic acid to the test tube.

[0014] Preferably, the precision control pen has a delivery hole for drawing hyaluronic acid. On both sides of the delivery hole, there are symmetrically distributed cleaning holes and drying holes. The cleaning holes are used to deliver cleaning fluid, and the cleaning fluid enters the delivery hole along the cleaning holes to rinse the material to be tested remaining on the inner wall of the delivery hole. The drying holes are used to deliver gas, and high-pressure gas enters the delivery hole along the drying holes to dry the cleaning fluid remaining on the inner wall of the delivery hole. The switching between cleaning fluid and high-pressure gas is achieved through a multi-channel valve.

[0015] Preferably, the mounting bracket includes a vertical partition that isolates and seals the detection cavity, a sealing ring is installed on one side of the vertical partition, and the side of the vertical partition with the sealing ring is movably fitted onto the detection cavity of the detection chamber.

[0016] The vertical partition is also equipped with several sets of mounting plates at equal intervals along the height direction. The mounting plates have several mounting holes for inserting test tubes and filling hyaluronic acid. The mounting holes of the lower mounting plates are smaller than the diameter of the mounting holes on the other mounting plates.

[0017] Preferably, the multi-channel valve has a dedicated No. 1 port for conveying hyaluronic acid in the middle, which is connected to the conveying port. The multi-channel valve has a No. 2 and a No. 3 groove with an i-shaped structure on both sides, which are staggered and distributed. The No. 2 groove is connected to the cleaning port, and the No. 3 groove is connected to the drying port.

[0018] Preferably, an air pump that delivers gas through a pump body is installed in the working inner cavity of the detection chamber. The air pump is connected to an air pressure pipe that delivers high-pressure gas, and one end of the air pressure pipe extends to a multi-channel valve and is connected to its third slot. The third slot of the multi-channel valve is connected to the drying hole of the precision control pen.

[0019] Preferably, the working inner cavity of the detection chamber is also equipped with a cleaning chamber that delivers cleaning fluid through an infusion pump. The cleaning chamber is connected to an infusion tube via the infusion pump, and one end of the infusion tube extends to a multi-channel valve and is connected to the second slot. The second slot of the multi-channel valve is connected to the cleaning port of the precision control pen.

[0020] Preferably, the nozzle is also provided with a switching component, which includes a moving block that moves at multiple points on the cross control system. A switching shaft is rotatably mounted on the bottom of the moving block via a bearing. The nozzle is mounted on the switching shaft, and a servo motor is connected to one side of the switching shaft via a motor mount.

[0021] The moving block and the nozzle mounted on its upper end are controlled to switch 90 degrees along the rotation direction of the servo motor output shaft.

[0022] Preferably, the precision control pen is also equipped with a main control unit, which includes a No. 1 button and a No. 2 button that slide through the side wall. The No. 1 button passes through the precision control pen and extends to the drying hole inside it. The drying hole is equipped with a No. 1 passive contact piece and a No. 2 passive contact piece. The side wall of the No. 1 button is equipped with a No. 1 active contact piece and a No. 2 active contact piece. The No. 1 passive contact piece contacts the No. 1 active contact piece to trigger high-pressure gas, and the No. 2 passive contact piece contacts the No. 2 active contact piece to trigger cleaning fluid operation.

[0023] The precision control pen has a sliding sealing block inside both the cleaning hole and the drying hole, and a return spring is installed on the sealing block. An electromagnetic suction block is installed on the inner wall of the precision control pen.

[0024] Preferably, the recessed area on the outside of the detection chamber is provided with a tray with a U-shaped structure, and the edge of the tray is provided with a spray nozzle for spraying cleaning fluid and a jet nozzle for spraying high-pressure gas.

[0025] Secondly, this application provides a method for detecting the purity of hyaluronic acid, comprising:

[0026] S1. Device preparation: Pull out the mounting bracket, place the test tube on the mounting bracket and push it back into the detection chamber; place the precision controller on the detection chamber;

[0027] S2. Sampling and delivery: Remove the precision controller from the detection chamber, and at the same time, the cross control system moves the nozzle to the top of the test tube; then, hold the precision controller and insert it into the hyaluronic acid to be tested to extract a quantitative amount of hyaluronic acid, which is then delivered into the test tube through the nozzle.

[0028] S3. Cleaning and Drying: The precision controller that absorbs hyaluronic acid is placed back into the groove of the test chamber. Then, the cleaning solution is used to rinse the delivery port, and then the high-pressure gas is triggered to dry the delivery port, which makes it easier to extract other materials to be tested.

[0029] S4. Sample detection: After the sample has settled, the cross control system adjusts the detector position, starts the detector detection, and records the data.

[0030] S5. Cleaning and Resetting: Then clean the outside of the precision control pen; pull out the mounting bracket to remove the test tube, clean the inner cavity, reset, and turn off the power.

[0031] In summary, this application includes at least one of the following beneficial technical effects:

[0032] I. This invention, by setting a recessed precision controller on one side of the detection chamber, combined with the delivery hole inside the precision control pen, symmetrical cleaning holes and drying holes, and the switching control of cleaning fluid and high-pressure gas by a multi-channel valve, can thoroughly remove residual samples and cleaning fluid from the inner wall of the delivery hole, avoid cross-contamination, and significantly improve sampling and detection accuracy.

[0033] Second, this invention enables rapid loading and unloading of test tubes through a pull-out mounting bracket and a mounting plate with elastic mounting holes; it relies on a cross control system to drive the detector and nozzle to move precisely, and combines a switching component to drive the nozzle to switch positions at 90 degrees, reducing manual operation steps and greatly improving testing efficiency.

[0034] Third, the precision controller of the present invention can not only automatically draw up the hyaluronic acid to be tested, but also quickly extract and test hyaluronic acid at different depths and in different areas. Moreover, after extracting hyaluronic acid, the precision controller can automatically perform a cleaning operation to avoid cross-contamination between the hyaluronic acid to be tested. Attached Figure Description

[0035] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0036] Figure 1 This is a schematic diagram of the main structure of the present invention.

[0037] Figure 2 This is a schematic diagram of the internal structure of the detection chamber of the present invention.

[0038] Figure 3 This is a schematic diagram of the mounting bracket of the present invention.

[0039] Figure 4 This is a first-view structural diagram of the working cavity, precision controller, and cross control system of the present invention.

[0040] Figure 5This is a second-view structural diagram of the working cavity, precision controller, and cross control system of the present invention.

[0041] Figure 6 This is a schematic diagram of the structure of the precision controller of the present invention.

[0042] Figure 7 This is a schematic diagram of the structure of the air pump, air pressure pipe, cleaning box and infusion pipe of the present invention.

[0043] Figure 8 This is a schematic diagram of the switching component of the present invention.

[0044] Figure 9 This is a schematic diagram of the structure of the wastewater collection tank, collection pipe, collection interface, and telescopic spring of the present invention.

[0045] Figure 10 This is a schematic diagram of the structure between the switching component and the collection interface of the present invention.

[0046] Figure 11 This is a schematic diagram of the structure between the conveying hole, the cleaning hole, and the second and third grooves of the present invention.

[0047] Figure 12 This is a first-view structural diagram of the main control of the present invention.

[0048] Figure 13 This is a schematic diagram of the second-view structure of the main control of this invention.

[0049] Figure 14 This is the present invention. Figure 13 Enlarged view of the local structure at point A in the image.

[0050] Figure 15 This is a flowchart of a method for detecting the purity of hyaluronic acid according to the present invention.

[0051] Explanation of reference numerals in the attached figures:

[0052] 1. Inspection chamber; 10. Inspection cavity; 2. Mounting bracket; 11. Working cavity; 3. Precision controller; 4. Cross control system; 5. Detector; 30. Precision control pen; 31. Multi-channel valve; 32. Conveying port; 33. Cleaning port; 34. Drying port; 36. Control tube; 37. Nozzle; 20. Vertical partition; 21. Sealing ring; 22. Mounting plate; 23. Mounting hole; 310. Hole No. 1; 311. Slot No. 2; 312. Slot No. 3; 40. Air pump; 41. Air pressure pipe; 50. 51. Cleaning tank; 6. Infusion tube; 72. Switching component; 8. Moving block; 9. Switching shaft; 10. Servo motor; 11. Wastewater collection tank; 12. Collection pipe; 13. Collection interface; 14. Telescopic spring; 15. Main control; 16. Button 1; 17. Button 2; 18. Passive contact plate 1; 19. Passive contact plate 2; 10. Active contact plate 1; 10. Active contact plate 2; 11. Sealing block; 12. Electromagnetic suction block; 13. Support tray; 14. Injection nozzle; 15. Air jet nozzle. Detailed Implementation

[0053] The following combination Figures 1-15 This application will be described in further detail.

[0054] This application discloses a hyaluronic acid purity detection device and detection method, and firstly relates to a hyaluronic acid purity detection device.

[0055] Existing hyaluronic acid testing equipment typically uses a handheld sampling method. After the pipette and sampling needle are removed from the equipment, the sample needs to be manually transferred to a test tube in the fixed testing chamber. During manual operation, factors such as hand tremors and visual deviations can cause sample spillage or misalignment between the nozzle 37 and the test tube opening, which not only wastes the sample but also introduces external contamination.

[0056] Therefore, this application proposes a hyaluronic acid purity detection device to solve this problem.

[0057] Reference Figure 1 , Figure 2 and Figure 3 As shown, a hyaluronic acid purity testing device includes a stationary testing chamber 1. The testing chamber 1 has a testing cavity 10 that opens to one side and communicates with the outside. The testing cavity 10 is slidably connected to a mounting bracket 2 for loading test tubes. The testing chamber 1 also has a working cavity 11.

[0058] A detector 5 is also provided on the inner wall of the detection box 1, which is connected to the cross control system 4.

[0059] It should be noted that the testing chamber 1, as the main supporting structure of the device, is made of a material with excellent corrosion resistance. It not only has high strength characteristics, providing a stable installation foundation for the various components of the device, but also resists the erosion of chemicals such as hyaluronic acid samples and cleaning solutions, thus extending the overall service life of the device.

[0060] The testing chamber 1 has two functionally independent inner cavities, namely the testing inner cavity 10 and the working inner cavity 11. The two inner cavities are separated by a sealing partition to avoid mutual interference.

[0061] The inner cavity 10 is mainly used to place the mounting bracket 2 for loading test tubes, providing a relatively closed and clean environment for sample testing.

[0062] The working cavity 11 is used to install auxiliary equipment such as air pump 40, cleaning chamber 50, and wastewater collection tank 70, ensuring that the operation of these devices does not affect the stability of the testing process.

[0063] The exterior of the testing chamber 1 is also equipped with an operation panel and a display screen. The operation panel is equipped with controls such as a power switch and function switching buttons, which makes it easy for operators to control the operation of the device.

[0064] This hyaluronic acid purity testing device aims to achieve accurate and efficient detection of the purity of hyaluronic acid samples. The device uses the testing chamber 1 as its core supporting structure, integrating a mounting bracket 2 for loading test tubes, a precision controller 3 for quantitative extraction and delivery of hyaluronic acid, and a cross-shaped control system 4 for precisely moving the detector 5 and nozzle 37. It can complete the entire process from sample collection, delivery, cleaning and drying, to sample testing and equipment cleaning and reset.

[0065] Reference Figure 3 As shown, before conducting a purity test on hyaluronic acid, preparatory work is required. The test tube containing the hyaluronic acid is installed inside the entire testing chamber 1 to facilitate the purity testing of multiple sets of hyaluronic acid. Specifically, the mounting bracket 2 includes a vertical partition 20 that isolates and seals the inner cavity 10 of the testing chamber. A sealing ring 21 is installed on one side of the vertical partition 20, and the side of the vertical partition 20 with the sealing ring 21 is movably attached to the inner cavity 10 of the testing chamber 1.

[0066] The vertical partition 20 is also equipped with several sets of mounting plates 22 at equal intervals along the height direction. The mounting plates 22 have several mounting holes 23 for inserting test tubes and filling hyaluronic acid. The mounting holes 23 on the lower mounting plate 22 are smaller than the diameter of the mounting holes 23 on the other mounting plates 22.

[0067] The mounting bracket 2 is mainly composed of a vertical partition 20 and a mounting plate 22. The vertical partition 20 is made of high-strength engineering plastic material, which is lightweight and has good structural stability. This material can reduce the overall weight of the mounting bracket 2, making it easier for operators to pull it out, and can also prevent the guide rail of the inner cavity 10 from being worn due to excessive material weight.

[0068] A sealing ring 21 is installed on one side of the vertical partition 20. The sealing ring 21 is made of rubber and has excellent aging resistance and sealing performance. The sealing ring 21 is embedded into the edge of the vertical partition 20 through a groove, and its surface is in close contact with the opening edge of the detection cavity 10.

[0069] Along the height direction of the vertical partition 20, several sets of mounting plates 22 are installed at equal intervals. The mounting plates 22 are detachably connected to the vertical partition 20 by buckles. This connection method makes it easy for operators to replace mounting plates 22 of different heights according to the test tube length, thereby improving the adaptability of the mounting bracket 2.

[0070] In practice, first, grasp the handle on the vertical partition 20 and pull the mounting plate 22 out of the detection chamber 1. At this time, the mounting holes 23 on the mounting plate 22 are exposed. Then, adjust the height between the mounting plates 22 according to the size of the test tubes. Next, insert the test tubes into the mounting holes 23 of the mounting plates 22 one by one. Then, push the mounting plates 22, test tubes, and vertical partition 20 back into the detection chamber 1. When the mounting bracket 2 is pushed back into the detection chamber 10, the sealing ring 21 abuts against the side wall of the detection chamber 1. The sealing ring 21 can isolate and seal the detection chamber 10 from the external environment, preventing external dust and impurities from entering the detection chamber 10 and contaminating the sample. At the same time, it avoids the leakage of volatile substances in the detection chamber 10, ensuring the health and safety of the operators.

[0071] Once the test tube is installed inside the detection chamber 1, the hyaluronic acid to be tested can be extracted. The main component for extracting hyaluronic acid in this application is the precision controller 3. The precision controller 3 is the core component for realizing the quantitative extraction and transportation of hyaluronic acid samples, ensuring accurate sample volume, no leakage during transportation, and no residue during cleaning, as detailed below:

[0072] Reference Figure 4 , Figure 5 and Figure 6As shown, a precision controller 3 for precisely drawing and quantifying hyaluronic acid is provided on one side of the detection chamber 1; an inward recess is provided on one side of the detection chamber 1 to house the precision controller 3. The precision controller 3 includes a movable precision pen 30 and a multi-channel valve 31 mounted on the top of the precision pen 30. The precision pen 30 has a delivery hole 32 for drawing hyaluronic acid. On both sides of the delivery hole 32 of the precision pen 30, there are symmetrically distributed cleaning holes 33 and drying holes 34. The cleaning holes 33 are used for delivering cleaning fluid, and the cleaning fluid enters the delivery hole 32 along the cleaning holes 33 to rinse the material to be tested remaining on the inner wall of the delivery hole 32. The drying holes 34 are used for delivering gas, and high-pressure gas enters the delivery hole 32 along the drying holes 34 to dry the cleaning fluid remaining on the inner wall of the delivery hole 32. The smooth delivery of cleaning fluid and high-pressure gas is achieved by switching through the multi-channel valve 31.

[0073] The multi-channel valve 31 is also equipped with a control tube 36, one end of which is connected to a nozzle 37 for delivering hyaluronic acid to the test tube. One end of the control tube 36 is connected to the multi-channel valve 31, and the other end is connected to the nozzle 37.

[0074] The Precision Pen 30 features a cylindrical structure design, with its overall length and diameter conforming to ergonomic principles, making it easy for operators to hold and operate.

[0075] The precision control pen 30 has a delivery hole 32 along its axis inside. The delivery hole 32 is the main delivery channel for hyaluronic acid samples. The inlet end of the delivery hole 32 has a tapered structure, which makes it easy for the precision control pen 30 to be inserted into the hyaluronic acid sample to be tested, while avoiding sample accumulation at the inlet.

[0076] Along both sides of the delivery port 32, cleaning ports 33 and drying ports 34 are symmetrically distributed. After the cleaning fluid enters the delivery port 32 through the cleaning port 33, it forms a spiral flushing flow, fully covering the inner wall of the delivery port 32 and improving the flushing effect. After the high-pressure gas enters through the drying port 34, it can also form a uniform airflow along the inner wall of the delivery port 32, quickly drying the residual cleaning fluid. This facilitates the extraction of different hyaluronic acid samples by the precision control pen 30 and avoids contamination between multiple sets of hyaluronic acid samples.

[0077] Reference Figure 5 , Figure 6 and Figure 7 As shown, the multi-channel valve 31 has a first hole 310 in the middle for conveying hyaluronic acid. The first hole 310 is connected to the conveying hole 32. The two sides of the multi-channel valve 31 are respectively provided with a second groove 311 and a third groove 312 with a U-shaped structure. The second groove 311 and the third groove 312 are distributed in a staggered manner. The second groove 311 is connected to the cleaning hole 33, and the third groove 312 is connected to the drying hole 34.

[0078] The multi-channel valve 31 is installed on the top of the precision control pen 30 and is made of high-strength ceramic material. The ceramic material has excellent sealing performance, wear resistance and chemical stability. Its main purpose is to ensure the isolation and separation of the cleaning hole 33 and the drying hole 34 to prevent the two from mixing. In addition, multiple pipes are connected to the multi-channel valve 31. The pipes are vulnerable parts, and the multi-channel valve 31 improves the efficiency of their replacement.

[0079] The multi-channel valve 31 has a second groove 311 and a third groove 312 with a U-shaped structure on both sides. The second groove 311 is connected to the cleaning hole 33 and is used to transport the cleaning fluid; the third groove 312 is connected to the drying hole 34 and is used to transport high-pressure gas. The second groove 311 and the third groove 312 are designed to be staggered, which can prevent the cleaning fluid and high-pressure gas from mixing during the channel switching process.

[0080] Reference Figure 8 , Figure 9 and Figure 10 As shown, specifically, the nozzle 37 is also provided with a switching component 6. The switching component 6 includes a moving block 60 that moves at multiple points on the cross control system 4. The bottom of the moving block 60 is rotatably mounted with a switching shaft 61 via a bearing. The nozzle 37 is mounted on the switching shaft 61. A servo motor 62 is connected to one side of the switching shaft 61 via a motor mount.

[0081] The moving block 60 and the nozzle 37 mounted on its upper end are controlled to switch 90 degrees along the rotation direction of the output shaft of the servo motor 62.

[0082] The cross control system 4 and the detector 5 together constitute the core execution unit for sample detection. The cross control system 4 is responsible for driving the detector 5 and the nozzle 37 to achieve multi-dimensional precise movement, ensuring that the detector 5 can be accurately aligned with the sample and the nozzle 37 can be accurately aligned with the test tube opening. The detector 5 is responsible for collecting the detection signal of the sample, providing data support for the calculation of hyaluronic acid purity.

[0083] The cross control system 4 is mainly composed of X-axis guide rail, Y-axis guide rail and drive components. The guide rails are perpendicular to each other and realize horizontal and vertical movement respectively. They work together to drive the detector 5 and the nozzle 37 to any position within the detection range.

[0084] Detector 5 is a high-performance liquid chromatography detector 5. This type of detector 5 has the characteristics of high separation efficiency, high detection sensitivity and fast analysis speed. It can accurately detect the impurity content in hyaluronic acid samples, thereby calculating the purity of hyaluronic acid, which meets the technical requirements for hyaluronic acid purity detection.

[0085] Detector 5 is a known existing structure, mainly composed of a detection probe, an optical system, and a signal processing unit. The detection probe is the key component of detector 5 that contacts the sample. It is made of quartz material, which has good light transmittance, ensuring that the detection signal passes smoothly through the probe and the sample. At the same time, quartz material has excellent chemical stability and will not react with the sample. The detection probe is designed in a cylindrical shape, and its end is specially polished to reduce signal reflection interference.

[0086] The signal processing unit filters and performs analog-to-digital conversion on the electrical signal output by the photomultiplier tube, converting the analog signal into a digital signal, and then transmits it to the device's data analysis system. The data analysis system calculates the impurity content in the hyaluronic acid sample based on the intensity of the digital signal, combined with a preset standard curve and calculation model, thereby determining the purity of the hyaluronic acid, and storing and displaying the test results on the display screen.

[0087] Reference Figure 11 , Figure 12 , Figure 13 and Figure 14 As shown, specifically, the precision control pen 30 is also equipped with a main control 8. The main control 8 includes a first button 80 and a second button 81 that slide through the side wall. The first button 80 passes through the precision control pen 30 and extends to the drying hole 34 inside it. The drying hole 34 is equipped with a first passive contact 82 and a second passive contact 83. The side wall of the first button 80 is equipped with a first active contact 84 and a second active contact 85. The first passive contact 82 contacts the first active contact 84 to trigger high-pressure gas, and the second passive contact 83 contacts the second active contact 85 to trigger cleaning fluid operation.

[0088] The precision control pen 30 has a sealing block 86 slidably installed in both the cleaning hole 33 and the drying hole 34. The sealing block 86 is equipped with a reset spring. The precision control pen 30 has an electromagnetic suction block 87 installed on its inner wall.

[0089] The outer recess of the detection chamber 1 is provided with a U-shaped support tray 90, and the edge of the support tray 90 is provided with a liquid spray nozzle 91 for spraying cleaning fluid and a gas spray nozzle 92 for spraying high-pressure gas.

[0090] The core function of the switching component 6 is to enable the nozzle 37 to switch precisely between the two stations of "sample delivery" and "wastewater collection", ensuring that the wastewater generated during the cleaning process can flow smoothly into the wastewater collection tank 70, and avoiding wastewater contamination of the test tube or the inner cavity of the test chamber 10.

[0091] The switching shaft 61 is rotatably mounted on the bottom of the movable block 60 via bearings. The bearings ensure smooth and stable rotation of the switching shaft 61, reducing resistance and noise during rotation. The nozzle 37 is fixed to the lower end of the switching shaft 61 by a locking nut. The locking nut has an anti-loosening design to prevent the nozzle 37 from loosening due to vibration during use. A sealing gasket is provided between the nozzle 37 and the switching shaft 61 to ensure that cleaning fluid or samples do not leak from the connection.

[0092] In practice, when it is necessary to test the purity of the hyaluronic acid to be tested, the control pen 30 is first removed from the test chamber 1, and then the control pen 30 is inserted into the hyaluronic acid to be tested. Then, the second button 81 is pressed. At this time, the second button 81 powers on the control pen 30, which then draws the hyaluronic acid through the delivery hole 32 and sprays it out from the nozzle 37 through the control tube 36, so that the hyaluronic acid is sprayed into the corresponding test tube. Then, the detector 5 above the test tube is activated to test the purity of the hyaluronic acid in the test tube.

[0093] It is important to note that when the hyaluronic acid in the test tube is being tested for purity, the control pen 30 should be reattached to the testing chamber 1, with the end of the control pen 30 that absorbs the hyaluronic acid touching the support tray 90. The spray nozzle 91 on the support tray 90 will then spray cleaning fluid downwards at an angle. The cleaning fluid will clean the end of the control pen 30 that absorbs the hyaluronic acid, preventing the hyaluronic acid from adhering to the surface of the control pen 30. Furthermore, after the cleaning fluid has cleaned the surface of the control pen 30, the air jet nozzle 92 will start working, and high-pressure gas will spray downwards at an angle onto the surface of the control pen 30, peeling the cleaning fluid off the surface of the control pen 30 and ensuring the cleanliness of the control pen 30.

[0094] Furthermore, pressing button 80 causes the first passive contact 82 and the first active contact 84 to come into contact. At this time, the sealing block 86 on the precision control pen 30 located in the cleaning hole 33 is attracted by the corresponding electromagnetic suction block 87. Then, the cleaning fluid cleans the delivery hole 32 of the precision control pen 30. Then, pressing button 80 again causes the second passive contact 83 and the second active contact 85 to come into contact, causing the sealing block 86 on the drying hole 34 of the precision control pen 30 to be attracted by the corresponding electromagnetic suction block 87. At this time, the drying hole 34 is connected to the delivery hole 32, and then high-pressure gas is sprayed from the drying hole 34 into the delivery hole 32.

[0095] It should be noted that button 80 has a two-stage structure. The first stage is used to clean the inner wall of the control pen 30 and the control tube 36, and the second stage is used to dry the inner wall of the control pen 30 and the control tube 36.

[0096] Looking back Figure 7As shown, a cleaning tank 50 is also installed in the working inner cavity 11 of the detection box 1, which delivers cleaning fluid through an infusion pump. The cleaning tank 50 is connected to an infusion pipe 51 through the infusion pump, and one end of the infusion pipe 51 extends to the multi-channel valve 31 and is connected to the second slot 311. The second slot 311 of the multi-channel valve 31 is connected to the cleaning hole 33 of the precision control pen 30.

[0097] The cleaning chamber 50 and the infusion tube 51 provide cleaning fluid for the cleaning function of the precision controller 3, ensuring that the residual hyaluronic acid sample on the inner wall of the delivery hole 32 can be thoroughly rinsed away, avoiding cross-contamination between different samples.

[0098] The cleaning chamber 50 is installed in the working cavity 11 of the testing chamber 1. It is made of polyethylene, which has excellent chemical stability and can resist the corrosion of various cleaning solutions. At the same time, the material is lightweight and easy to process, making it suitable as a storage container for cleaning solutions. The top of the cleaning chamber 50 is equipped with a liquid filling port with a sealing cap. The sealing cap is threaded to prevent the cleaning solution from evaporating or external impurities from entering the chamber and contaminating the cleaning solution. The liquid filling port has a large diameter, which makes it easy for operators to add cleaning solution and also facilitates the cleaning of the inside of the cleaning chamber 50.

[0099] The cleaning tank 50 is equipped with a liquid level sensor, which can monitor the liquid level of the cleaning fluid in the tank in real time and transmit the liquid level signal to the control system of the device. When the liquid level of the cleaning fluid is lower than the preset lower limit, the control system will issue an alarm on the display screen to remind the operator to add cleaning fluid in time.

[0100] The cleaning chamber 50 is connected to the infusion tube 51 via an infusion pump. The infusion pump is a miniature peristaltic pump, which features high delivery accuracy, good sealing, and no contamination. The peristaltic pump delivers liquid by squeezing the infusion tube 51. The liquid only contacts the inner wall of the infusion tube 51 and does not contact the internal components of the pump body, thus avoiding contamination of the cleaning solution by the pump body. At the same time, the peristaltic pump can precisely control the delivery flow rate and volume of the cleaning solution by adjusting the speed. The operator can set appropriate delivery parameters according to factors such as the size of the delivery hole 32 and the amount of residual sample to ensure that the cleaning solution can thoroughly rinse the residual sample without waste.

[0101] In practice, first press button 80 to trigger the first stage of button 80. At this time, the infusion pump inside the cleaning tank is powered on and starts to deliver the cleaning solution through the infusion tube 51 to the cleaning hole 33 of the precision control pen 30. Then the cleaning solution passes through the sealing block 86 of the cleaning hole 33 and enters the delivery hole 32 of the precision control pen 30. Then the residual hyaluronic acid on the inner wall of the delivery hole 32 is rinsed clean. The wastewater after rinsing is sprayed out from the nozzle 37 and then collected.

[0102] Let's look again. Figure 7As shown, specifically, an air pump 40 is installed in the working cavity 11 of the detection chamber 1 to deliver gas through the pump body. The air pump 40 is connected to an air pressure pipe 41 for delivering high-pressure gas, and one end of the air pressure pipe 41 extends to the multi-channel valve 31 and is connected to its third slot 312. The third slot 312 of the multi-channel valve 31 is connected to the drying hole 34 of the precision control pen 30.

[0103] The air pump 40 and the air pressure pipe 41 together provide high-pressure gas for the drying function of the precision controller 3, ensuring that the cleaning liquid remaining on the inner wall of the delivery hole 32 can be thoroughly dried, and avoiding the residual cleaning liquid from affecting subsequent sample testing.

[0104] The air pump 40 is installed in the working cavity 11 of the test chamber 1. It is an oil-free silent air pump 40. The oil-free design can prevent oil stains generated during the operation of the air pump 40 from entering the delivery hole 32 with the gas.

[0105] In practice, after the cleaning fluid has cleaned the delivery hole 32 of the precision control pen 30, the first button 80 is pressed to trigger the second stage of the first button 80. At this time, the air pump 40 is powered on and starts. The air pump 40 delivers high-pressure gas through the air pressure pipe 41 to the drying hole 34 of the precision control pen 30. Then, the high-pressure gas enters the delivery hole 32 of the precision control pen 30 through the drying hole 34 to dry the cleaning fluid remaining on the inner wall of the delivery hole 32 and the control pipe 36, so that it enters the wastewater collection tank 70 for unified collection.

[0106] Reference Figure 7 , Figure 8 and Figure 9 As shown, specifically, a wastewater collection tank 70 is also installed in the working cavity 11 of the detection box 1. A collection pipe 71 is installed on the wastewater collection tank 70. One end of the collection pipe 71 extends to the vicinity of the moving block 60, and a collection interface 72 that is slidably connected to the moving block 60 is connected to the collection pipe 71. The end of the collection interface 72 near the switching shaft 61 is provided with a large inclined leak-proof opening.

[0107] A telescopic spring 73 connects the collection interface 72 to the moving block 60.

[0108] The wastewater collection tank 70, collection pipe 71, collection interface 72 and telescopic spring 73 together constitute a wastewater collection system, which is responsible for collecting the wastewater generated during the cleaning process, avoiding wastewater pollution of equipment or environment, and ensuring the smoothness and reliability of the wastewater collection process.

[0109] One end of the collection pipe 71 connects to the collection pipe 71 interface of the wastewater collection tank 70, and the other end extends to the vicinity of the movable block 60, serving as the conveying channel for wastewater to flow from the collection interface 72 into the wastewater collection tank 70. The collection pipe 71 is made of polypropylene, which possesses good chemical corrosion resistance and toughness, allowing for flexible arrangement within the working cavity 11 and preventing breakage due to aging. The smooth inner wall of the collection pipe 71 reduces wastewater residue inside the pipe, preventing bacterial growth or odor. All connections between the collection pipe 71 and the wastewater collection tank 70, as well as the collection interface 72, are sealed to ensure no wastewater leakage.

[0110] The collection interface 72 has a large, inclined, leak-proof opening at one end near the switching shaft 61. The angle of the opening is optimized to ensure that the outlet of the nozzle 37 is perfectly aligned with the opening of the collection interface 72 when the nozzle 37 is rotated to the cleaning station, while also preventing wastewater from overflowing during the inflow process. The inner wall of the collection interface 72 is polished to reduce wastewater residue. The other end of the collection interface 72 is connected to the collection pipe 71, and the collection interface 72 is slidably connected to the moving block 60. The collection interface 72 has a slider, and the moving block 60 has a corresponding slide rail. The slider can slide freely along the slide rail, allowing the collection interface 72 to move together with the moving block 60. This ensures that when the moving block 60 moves the nozzle 37, the collection interface 72 always maintains a proper relative position with the nozzle 37, preventing misalignment between the collection interface 72 and the nozzle 37 due to the movement of the moving block 60, which would affect wastewater collection.

[0111] Reference Figure 15 As shown, this application also provides a method for detecting the purity of hyaluronic acid, as detailed below:

[0112] S1. Device preparation: Pull out the mounting bracket 2 along the length of the detection chamber 10 of the detection box 1. Select the mounting plate 22 with the corresponding diameter mounting hole 23 according to the size of the test tube. After filling the test tube with the hyaluronic acid to be tested, push the mounting bracket 2 back into the detection chamber 10 so that the vertical partition 20 is sealed to the detection chamber 10 through the sealing ring 21. At the same time, place the precision controller 3 in the recess on one side of the detection box 1 and lock it onto the U-shaped support tray 90.

[0113] S2. Sampling and delivery: Operate the main control 8 on the precision control pen 30. In the initial state, the multi-channel valve 31 is switched to the first hole 310 in the middle. Press the initial position of the main control 8 to drive the precision control pen 30 to draw a quantitative amount of hyaluronic acid through the delivery hole 32. The cross control system 4 controls the moving block 60 to move the nozzle 37 to directly above the target test tube. The multi-channel valve 31 keeps the first hole 310 open. The hyaluronic acid is delivered into the test tube through the delivery hole 32, the control tube 36 and the nozzle 37.

[0114] S3. Cleaning and Drying: After a single sampling and delivery, the servo motor 62 is started to drive the switching shaft 61 to rotate the nozzle 37 by 90°, so that the nozzle 37 is aligned with the inclined large opening of the collection interface 72; press the first button 80 to trigger the cleaning chamber 50 to deliver cleaning fluid to the second tank 311 of the multi-channel valve 31 through the infusion pump and infusion pipe 51. The cleaning fluid enters the delivery hole 32 of the precision control pen 30 through the cleaning hole 33 to rinse the residual material to be tested on the inner wall. The rinsing wastewater flows into the collection interface 72 through the nozzle 37 and then into the wastewater collection tank 70 through the collection pipe 71; after cleaning, press the first button 80 again to trigger the second stage, triggering the air pump 40 to deliver high-pressure gas to the third tank 312 of the multi-channel valve 31 through the air pressure pipe 41. The high-pressure gas enters the delivery hole 32 through the drying hole 34 to dry the residual cleaning fluid on the inner wall.

[0115] S4. Cleaning and Drying: Sample Detection: After the hyaluronic acid sample in the test tube has been left to stand for the set time, the position of the detector 5 on the inner wall of the detection chamber 1 is adjusted by the cross control system 4 so that the detector 5 is aligned with the sample detection area in the test tube. The detector 5 is started according to the set parameters to detect the purity of the hyaluronic acid and the detection data is recorded.

[0116] S5. Cleaning and Reset: After all tests are completed, move the precision control pen 30 above the support tray 90, spray cleaning fluid through the spray nozzle 91 on the edge of the support tray 90 and spray high-pressure gas through the air nozzle 92 to clean the outside of the precision control pen 30; then pull out the mounting bracket 2, take out the test tube, clean the inner cavity 10 of the test, push the mounting bracket 2 back, and turn off the power of the device to complete the reset.

[0117] The embodiments described herein are preferred embodiments of the present invention and are not intended to limit the scope of protection of the present invention. Therefore, all equivalent changes made in accordance with the structure, shape, and principle of the present invention should be covered within the scope of protection of the present invention.

Claims

1. A device for detecting the purity of hyaluronic acid, characterized in that: include; The test chamber (1) has a test cavity (10) that opens to one side and communicates with the outside. The test cavity (10) is slidably connected to a mounting bracket (2) for loading test tubes along its length. The test chamber (1) also has a working cavity (11). The detection chamber (1) has a precision controller (3) for precisely drawing and quantifying hyaluronic acid on one side; the detection chamber (1) has an inward recess on one side, which is used to house the precision controller (3). A detector (5) connected to a cross control system (4) is also provided on the inner wall of the detection box (1). The precision controller (3) includes a movable precision pen (30) and a multi-channel valve (31) installed on the top of the precision pen (30). The multi-channel valve (31) is also provided with a control tube (36), one end of which is connected to a nozzle (37) for delivering hyaluronic acid to the test tube. The precision control pen (30) is provided with a delivery hole (32) for drawing hyaluronic acid. On both sides of the delivery hole (32) of the precision control pen (30), there are also symmetrically distributed cleaning holes (33) and drying holes (34). The cleaning hole (33) is used for the delivery of cleaning liquid, and the cleaning liquid enters the delivery hole (32) along the cleaning hole (33) to rinse the material to be tested remaining on the inner wall of the delivery hole (32). The drying hole (34) is used for the delivery of gas, and the high-pressure gas enters the delivery hole (32) along the drying hole (34) to dry the cleaning liquid remaining on the inner wall of the delivery hole (32). The switching of cleaning liquid and high-pressure gas is achieved by a multi-channel valve (31). The multi-channel valve (31) has a first hole (310) in the middle for conveying hyaluronic acid. The first hole (310) is connected to the conveying hole (32). The two sides of the multi-channel valve (31) are respectively provided with a second groove (311) and a third groove (312) with a U-shaped structure. The second groove (311) and the third groove (312) are staggered. The second groove (311) is connected to the cleaning hole (33), and the third groove (312) is connected to the drying hole (34). The nozzle (37) is also provided with a switching component (6), which includes a moving block (60) that moves at multiple points on the cross control system (4). The bottom of the moving block (60) is rotatably mounted with a switching shaft (61) via a bearing. The nozzle (37) is mounted on the switching shaft (61). A servo motor (62) is connected to one side of the switching shaft (61) via a motor mount. The moving block (60) and the nozzle (37) mounted on its upper end are controlled to switch ninety degrees along the rotation direction of the output shaft of the servo motor (62); The recessed area outside the test chamber (1) is provided with a tray (90) of the shape of a wedge. The edge of the tray (90) is provided with a spray nozzle (91) for spraying cleaning fluid and a jet nozzle (92) for spraying high-pressure gas.

2. The hyaluronic acid purity detection device according to claim 1, characterized in that: The mounting bracket (2) includes a vertical partition (20) that isolates and seals the detection cavity (10). A sealing ring (21) is installed on one side of the vertical partition (20), and the side of the vertical partition (20) with the sealing ring (21) is movably attached to the detection cavity (10) of the detection box (1). The vertical partition (20) is also equipped with several sets of mounting plates (22) at equal intervals along the height direction. The mounting plates (22) have several mounting holes (23) for inserting test tubes and filling hyaluronic acid. The diameter of the mounting hole (23) on the lower mounting plate (22) is smaller than the diameter of the mounting hole (23) on the other mounting plates (22).

3. The hyaluronic acid purity detection device according to claim 1, characterized in that: An air pump (40) that delivers gas through a pump body is installed in the working inner cavity (11) of the test chamber (1). The air pump (40) is connected to a pressure pipe (41) that delivers high-pressure gas. One end of the pressure pipe (41) extends to a multi-channel valve (31) and is connected to its third slot (312). The third slot (312) of the multi-channel valve (31) is connected to the drying hole (34) of the precision control pen (30).

4. The hyaluronic acid purity detection device according to claim 1, characterized in that: The working inner cavity (11) of the detection box (1) is also equipped with a cleaning box (50) that delivers cleaning fluid through an infusion pump. The cleaning box (50) is connected to an infusion tube (51) through the infusion pump, and one end of the infusion tube (51) extends to the multi-channel valve (31) and is connected to the second slot (311). The second slot (311) of the multi-channel valve (31) is connected to the cleaning hole (33) of the precision control pen (30).

5. The hyaluronic acid purity detection device according to claim 1, characterized in that: The precision control pen (30) is also provided with a main control (8). The main control (8) includes a first button (80) and a second button (81) that slide through the side wall. The first button (80) passes through the precision control pen (30) and extends to the drying hole (34) inside it. The drying hole (34) is provided with a first passive contact piece (82) and a second passive contact piece (83). The side wall of the first button (80) is provided with a first active contact piece (84) and a second active contact piece (85). When the first passive contact piece (82) contacts the first active contact piece (84), it triggers high-pressure gas. When the second passive contact piece (83) contacts the second active contact piece (85), it triggers cleaning fluid operation. The cleaning hole (33) and drying hole (34) of the precision control pen (30) are both equipped with a sealing block (86), and a reset spring is provided on the sealing block (86). An electromagnetic suction block (87) is installed on the inner wall of the precision control pen (30).

6. A method for detecting the purity of hyaluronic acid, using the hyaluronic acid purity detection device according to any one of claims 1-5, characterized in that: S1. Device preparation: Pull out the mounting bracket (2), place the test tube on the mounting bracket (2) and push it back into the detection chamber (10); place the precision controller (3) on the detection box (1); S2. Sampling and delivery: Remove the precision controller (3) from the detection box (1), and at the same time, the cross control system (4) controls the moving block (60) to move the nozzle (37) to the top of the target test tube; then hold the precision controller (3) and insert it into the hyaluronic acid to be tested, extract a quantitative amount of hyaluronic acid, and send the hyaluronic acid into the test tube through the nozzle (37); S3, Cleaning and Drying: Place the precision controller (3) that absorbs hyaluronic acid back into the groove of the test chamber (1), then rinse the delivery hole (32) with cleaning fluid, and then trigger the high-pressure gas to dry the delivery hole (32) to facilitate the extraction of other materials to be tested; S4. Sample detection: After the sample has settled, the cross control system (4) adjusts the position of the detector (5), starts the detector (5) to detect and record the data. S5. Cleaning and Resetting: Then clean the outside of the precision control pen (30); pull out the mounting bracket (2) to remove the test tube, clean the inner cavity and reset, then turn off the power.

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