Low glycemic index sucrose granules for improving brain nerve function and preparation method and application thereof

Abstract

This invention discloses a low glycemic index (GI) sucrose granules for improving brain nerve function, its preparation method, and its applications, belonging to the field of biomedicine. Using sucrose as a carrier and sugarcane polyphenols as functional factors, this invention produces low GI sucrose granules with polyphenol-coated sucrose crystals. Experimental results show that the GI of these low GI sucrose granules is ≤45, causing no post-consumption blood glucose fluctuations, making them suitable for diabetic patients. Furthermore, these low GI sucrose granules can slowly release glucose and sugarcane polyphenols, effectively penetrating the blood-brain barrier, synergistically improving energy deficiency in brain neurons, inhibiting β-amyloid protein (Aβ) deposition, reducing neuroinflammation, and improving brain nerve activity. This invention provides a new adjunctive treatment for improving brain nerve function in diabetic patients, possessing significant practical application value and broad application prospects.

Description

Technical Field

[0001] This invention relates to the field of biomedicine, and in particular to a low glycemic index sucrose granule for improving brain nerve function, its preparation method, and its application. Background Technology

[0002] Diabetes mellitus is a chronic disease characterized by hyperglycemia, caused by absolute or relative insulin deficiency and impaired insulin utilization. In recent years, the incidence of diabetes has been increasing annually. Diabetic patients need to strictly limit their sugar intake, but the brain is highly dependent on glucose for energy, and long-term sugar deficiency can easily lead to neuronal damage and cognitive decline. For diabetic patients, consuming regular sucrose (glycemic index ≥ 65) can easily cause a sharp rise in blood sugar; while consuming low-glycemic index (GI) foods such as artificial sweeteners (e.g., aspartame) or dietary fiber does not cause a sharp rise in blood sugar, it cannot simultaneously meet the brain's energy needs and neuroprotective requirements. Furthermore, there are currently no effective dietary interventions for the accumulation of β-amyloid protein (Aβ) in neurodegenerative diseases such as Alzheimer's disease, and polyphenols such as tea polyphenols often have limited effects due to low bioavailability or lack of synergistic effects with glucose metabolism.

[0003] Therefore, there is an urgent need for a product with a low glycemic index that can provide energy to the brain, clear amyloid deposits, and improve brain nerve function. Summary of the Invention

[0004] The purpose of this invention is to provide a low glycemic index sucrose granule for improving brain nerve function, its preparation method, and its application, in order to solve the problems existing in the prior art. This invention uses sucrose as a carrier and sugarcane polyphenols as functional factors to prepare low glycemic index sucrose granules coated with polyphenols. These low glycemic index sucrose granules can effectively improve the energy deficiency of brain neurons in diabetic patients, inhibit the deposition of β-amyloid protein (Aβ), reduce neuroinflammation, and improve brain nerve activity.

[0005] To achieve the above objectives, the present invention provides the following solution:

[0006] This invention provides a method for preparing low glycemic index sucrose granules that improve brain nerve function, comprising the following steps:

[0007] Take sugarcane, wash it, extract the juice, and collect the sugarcane juice and sugarcane residue separately;

[0008] The sugarcane juice is purified of impurities, and then subjected to electrodialysis to obtain a sucrose solution.

[0009] The sugarcane bagasse was subjected to polyphenol enrichment and extraction to obtain a sugarcane polyphenol extract.

[0010] The sugarcane polyphenol extract is mixed with the sucrose solution to obtain a mixture. The mixture is then mixed with calcium chloride and compounded using a co-crystallization method to obtain the low glycemic index sucrose granules.

[0011] Furthermore, the polyphenol enrichment and extraction includes the following steps: mixing the sugarcane bagasse with an ethanol solution, extracting, filtering and collecting the filtrate, and concentrating to obtain the sugarcane polyphenol extract.

[0012] Furthermore, the extraction temperature is 45°C and the extraction time is 3 hours.

[0013] Furthermore, the concentration was carried out at a temperature of 70°C for 2 hours.

[0014] Furthermore, the mass ratio of the mixture to the calcium chloride is 50:1.

[0015] The present invention also provides a low glycemic index sucrose granules obtained according to the above preparation method.

[0016] The present invention also provides the application of the above-mentioned low glycemic index sucrose granules in the preparation of drugs that improve brain nerve function.

[0017] The present invention also provides a drug for improving brain nerve function, with the above-mentioned low glycemic index sucrose granules as the main active ingredient.

[0018] Furthermore, it also includes pharmaceutically acceptable excipients.

[0019] Furthermore, the dosage form of the drug is tablets, granules, capsules, or pills.

[0020] The present invention discloses the following technical effects:

[0021] This invention utilizes sucrose as a carrier and sugarcane polyphenols as a functional factor to produce low glycemic index (GI) sucrose granules coated with polyphenols. Experimental results show that these GI granules have a GI of ≤45, do not cause post-consumption blood glucose fluctuations, and are suitable for diabetic patients. Furthermore, these GI granules can slowly release glucose and sugarcane polyphenols, effectively penetrate the blood-brain barrier, synergistically improve energy deficiency in brain neurons, inhibit β-amyloid (Aβ) deposition, reduce neuroinflammation, and improve brain nerve activity. This invention provides a new adjunctive treatment for improving brain nerve function in diabetic patients, possessing significant practical application value and broad application prospects. Attached Figure Description

[0022] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0023] Figure 1 A schematic diagram illustrating the synthesis method of low glycemic index sucrose granules;

[0024] Figure 2 The figure shows the experimental results of polyphenols crossing the blood-brain barrier in low glycemic index sucrose granules.

[0025] Figure 3 Figure showing the experimental results of using low glycemic index sucrose particles to clear β-amyloid protein deposits in the brain. Detailed Implementation

[0026] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0027] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any stated value or intermediate value within a stated range, as well as each smaller range between any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0028] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0029] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0030] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0031] The low glycemic index (GI) sucrose granules of the present invention use refined sucrose (providing glucose molecules) as a carrier and load sugarcane polyphenols (≥3% w / w, including active ingredients such as ferulic acid and proanthocyanidins) as functional factors to form a polyphenol-coated sucrose crystal complex, which exerts a synergistic mechanism of dual physiological effects.

[0032] At the metabolic level, sugarcane polyphenols inhibit the activity of α-glucosidase and sucrase, delaying the breakdown of sucrose. After taking it, the GI value is ≤45, which can avoid postprandial blood sugar fluctuations and is suitable for diabetic patients. At the neuroprotective level, the low glycemic index sucrose particles slowly release glucose, allowing glucose to continuously cross the blood-brain barrier, improving neuronal energy deficiency. At the same time, sugarcane polyphenols effectively penetrate the blood-brain barrier, inhibit the deposition of β-amyloid protein (Aβ), reduce neuroinflammation, and improve brain nerve activity.

[0033] Example 1: Preparation of low glycemic index sucrose granules

[0034] Preparation process as follows Figure 1 As shown.

[0035] Sugarcane is harvested, thoroughly cleaned, pressed, and the juice is collected. The juice is then passed through a ceramic membrane (5nm) to remove impurities and through an electrodialysis unit (operating voltage 30V, flow rate 60L / h) to obtain a sucrose solution. For the pressed bagasse, 80% ethanol (by volume) is added for polyphenol extraction at 45℃ for 3 hours. The mixture is filtered to remove the bagasse, yielding a polyphenol extract. This extract is concentrated at 70℃ for 2 hours, and the ethanol is removed. The enriched polyphenol extract is mixed with the sucrose solution, and 2% calcium chloride (by mass) is added to chelate the sucrose and polyphenols. The sugar solution concentration is controlled at 75%, and the temperature at 65℃ for co-crystallization to obtain low-glycemic sucrose granules.

[0036] Example 2 Characterization of low glycemic index sucrose granules

[0037] 1. Composition of sugarcane polyphenols in low glycemic index sucrose granules

[0038] Nine major polyphenols and flavonoid monomers in sugarcane polyphenols were systematically identified using the HPLC internal standard method. The results are shown in Table 1.

[0039] Table 1. Statistics on the content of polyphenols and flavonoid monomers in low glycemic index sucrose granules.

[0040]

[0041]

[0042] 2. Glycemic index (GI) value of low glycemic index sucrose granules

[0043] Preparation method of mixture of sucrose and sugarcane polyphenols:

[0044] The pressed bagasse was added with 80% ethanol by volume for polyphenol extraction at 45°C for 3 hours. After filtration to remove the bagasse, the mixture was concentrated at 70°C for 2 hours to remove the ethanol and then freeze-dried to obtain the sugarcane polyphenol extract.

[0045] Dissolve 1000g of sucrose in water, then add 10g of sugarcane polyphenol extract, stir thoroughly for 1 hour to mix, and freeze dry to obtain a mixture of sucrose and sugarcane polyphenols.

[0046] Twelve healthy adult volunteers (half male and half female) were selected for the test. The volunteers were aged 23-33 years, with a mean age of 26.50 ± 4.12 years and a mean body mass index (BMI) of 20.72 ± 1.15 kg / m². 2 The participants were light manual laborers with no history of diabetes, other metabolic diseases, digestive system diseases, endocrine system diseases, or mental illnesses. The glycemic index (GI) test was conducted according to WS / T 652-2019, "Methods for Determination of Glycemic Index of Foods." All experiments related to this invention were approved by the ethics committee, and informed consent was obtained from the volunteers.

[0047] Twelve participants were randomly divided into three groups, adjusted for gender and age. Participants fasted from 8:00 PM the night before the test until the following morning, when fasting blood glucose was measured using the fingertip capillary method. They then consumed sucrose (50g of carbohydrates), a mixture of sucrose and sugarcane polyphenols (50g of carbohydrates), or low glycemic index sucrose granules (50g of carbohydrates). Blood glucose was measured at 15, 30, 45, 60, 90, and 120 minutes after each food intake using the fingertip capillary method. Blood glucose levels after consuming 50g of glucose were used as a control. The testing interval between the different foods was greater than 72 hours.

[0048] Calculate the area under the glycemic response curve for each subject to the two foods, and calculate the glycemic index (GI) using the following formula:

[0049]

[0050] Among them, AUC S Area under the blood glucose response curve after a meal for the food being tested; AUC G The area under the glucose response curve after a glucose meal is denoted as .

[0051] The results are shown in Table 2. The experimental results show that the low glycemic index sucrose granules prepared in Example 1 have the lowest GI value of 45. The low glycemic index sucrose granules prepared in this invention are low GI foods and are suitable for diabetics.

[0052] Table 2. Statistical results of glycemic index of different foods

[0053]

[0054]

[0055] Example 3: Animal experiment on the improvement of brain nerve function by low glycemic index sucrose granules

[0056] 1. Experimental Methods

[0057] Thirty-six mice were divided into three groups of 12 each. The first group was administered a mixture of 100 mg / kg sucrose and sugarcane polyphenols by gavage as SP. The second group was administered 100 mg / kg low glycemic index sucrose granules by gavage as SP-NDs. The third group was administered the same volume of physiological saline by gavage. The mice were sacrificed after 2 hours, dissected, and their organs and tissues were collected. The polyphenol content in each tissue was detected by the Folin-phenol method.

[0058] Forty-eight mice were divided into four groups of 12 each. Groups 1, 2, and 3 were used to induce an STZ-induced mouse model of oxidative damage to brain neurons, while group 4 served as a normal control group. Group 1 was administered 300 mg / kg sucrose by gavage; Group 2 was administered a mixture of 300 mg / kg sucrose and sugarcane polyphenols by gavage; Group 3 was administered 300 mg / kg low glycemic index sucrose granules by gavage; and Group 4 was administered an equal volume of physiological saline by gavage. After 8 weeks, all mice were connected to an EEG acquisition system to record EEG spectral data. Delta and Theta values ​​were analyzed using an EEG analysis module to assess brain neuron activity. After the tests, the mice were euthanized, and brain tissue was harvested. The morphology of astrocytes and glial cells was observed using an optical microscope, and the Aβ aggregation status was observed using a fluorescence microscope.

[0059] 2. Experimental Results

[0060] 2.1 Polyphenols in low glycemic index sucrose particles cross the blood-brain barrier

[0061] like Figure 2 As shown, combined with in vivo imaging technology, it was confirmed that sugarcane polyphenols can efficiently penetrate the blood-brain barrier, with a distribution rate of up to 28% in brain tissue.

[0062] 2.2 Low glycemic index sucrose particles clear β-amyloid (Aβ) deposits in the brain

[0063] like Figure 3As shown, green fluorescence represents Aβ expression. After intervention with low glycemic index sucrose particles, Aβ deposition in the mouse brain significantly disappeared.

[0064] 2.3 The effect of low glycemic index sucrose particles on improving brain nerve activity

[0065] After intervention with sucrose, sucrose + polyphenols, and low glycemic index sucrose granules in diabetic mice, the activity of the mice's brain nerves was detected using a mouse brain nerve signal recording system. It was found that the signal heatmap of low glycemic index sucrose granules was stronger. Based on the heatmap, Delta and Theta values ​​were obtained, as shown in Table 3. The Delta and Theta values ​​of low glycemic index sucrose granules were closer to those of normal mice, indicating that low glycemic index sucrose granules can improve the activity of brain nerve cells and alleviate brain nerve dysfunction.

[0066] Table 3. Delta and Theta values ​​of mice in different experimental groups.

[0067] Delta 1 1.68 1.51 1.06 Theta 1 1.58 1.49 1.15

[0068] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A method for preparing a low glycemic index sucrose particle, characterized by, Includes the following steps: Take sugarcane, wash it, extract the juice, and collect the sugarcane juice and sugarcane residue separately; The sugarcane juice is purified of impurities, and then subjected to electrodialysis to obtain a sucrose solution. The sugarcane bagasse was subjected to polyphenol enrichment and extraction to obtain a sugarcane polyphenol extract. The sugarcane polyphenol extract is mixed with the sucrose solution to obtain a mixture. The mixture is then mixed with calcium chloride and compounded by co-crystallization to obtain the low glycemic index sucrose granules. The polyphenol enrichment and extraction includes the following steps: mixing the sugarcane bagasse with an ethanol solution, extracting, filtering and collecting the filtrate, and concentrating to obtain the sugarcane polyphenol extract. The mass ratio of the mixture to the calcium chloride is 50:

1.

2. The preparation method according to claim 1, characterized in that, The extraction temperature was 45℃ and the time was 3 hours.

3. The preparation method according to claim 1, characterized in that, The concentration was carried out at a temperature of 70°C for 2 hours.

4. A low glycemic index sucrose granule obtained by the preparation method according to any one of claims 1-3.

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