Nanodispersion for improving hyperactivity, anxiety and memory and preparation method thereof

Abstract

This invention belongs to the field of nanoemulsion technology, specifically relating to a nanoemulsion for improving hyperactivity, anxiety, and memory, and its preparation method. The composition comprises the following raw materials: by weight, 8-27 parts of nutrient oils, 0.57-1.18 parts of emulsifier, 2-4 parts of processing aid, 0.71-2.58 parts of functional components, 0.01-0.02 parts of antioxidant, and 70-85 parts of water. The nutrient oils include DHA algal oil and salmon oil. The above raw materials exhibit a synergistic effect, as verified in open field experiments to improve hyperactivity in rats; in water maze experiments to improve memory in rats; and to increase 5-HT levels in rats; and in zebrafish anxiety model experiments to improve anxiety behavior in zebrafish. These experiments confirm that the nanoemulsion prepared by this invention can effectively improve hyperactivity, anxiety, and memory.

Description

Technical Field

[0001] This invention belongs to the field of nanoemulsion technology, specifically relating to a nanoemulsion for improving hyperactivity, anxiety, and memory, and its preparation method. Background Technology

[0002] In the field of child health, the causes of anxiety and hyperactivity in children are complex and multifaceted. From a neurobiological perspective, dopamine / norepinephrine can cause systemic dysregulation, leading to insufficient inhibitory control function in the prefrontal cortex, manifesting as inattention and impulsive behavior. Anxiety disorders are associated with polymorphisms in the serotonin transporter (SERT) gene and abnormalities in the amygdala-prefrontal neural circuit, causing hypervigilance and mood regulation disorders. Among environmental factors, unstable family environments, inappropriate parenting styles (such as over-control or neglect of emotions), academic pressure, and social setbacks can all induce symptoms. Psychologically, children's own sensitivity, negative cognitive biases, and low self-efficacy can also exacerbate anxiety and hyperactivity tendencies. These multidimensional factors interact, making the prevention and intervention of childhood anxiety and hyperactivity problems face many challenges.

[0003] DHA (docosahexaenoic acid), an important omega-3 polyunsaturated fatty acid, plays a crucial role in brain development and nerve function regulation. EPA, the abbreviation for eicosapentaenoic acid, is a major component of fish oil and also belongs to the omega-3 polyunsaturated fatty acid family, making it an essential nutrient for the human body.

[0004] However, due to the reactive chemical properties of polyunsaturated fatty acids such as DHA and EPA, they are easily oxidized, resulting in poor stability and low bioavailability. Traditional dosage forms, such as soft capsules and oral liquids, cannot effectively solve this problem. Nanoemulsion technology can disperse the oil phase into nanoscale droplets, improving its stability and bioavailability. Summary of the Invention

[0005] To this end, the present invention provides a nanoemulsion prepared by rationally combining DHA algal oil, salmon oil, emulsifier, and functional ingredients. This nanoemulsion can effectively improve symptoms of hyperactivity and anxiety in children, while also improving memory. It also has a good taste and stability, making it easy for children to accept and take.

[0006] On the other hand, the present invention also provides a method for preparing the nanoemulsion, which is simple and easy to industrialize.

[0007] To solve the above-mentioned technical problems, the technical solution of the present invention is as follows:

[0008] On the one hand, the present invention provides a nanoemulsion for improving hyperactivity, anxiety and enhancing memory, comprising the following components by weight: 8-27 parts of nutritional oils, 0.57-1.18 parts of emulsifier, 2-4 parts of processing aid, 0.71-2.58 parts of functional ingredients, 0.01-0.02 parts of antioxidant and 70-85 parts of water.

[0009] Preferably, the nutritional oils include DHA algal oil and salmon oil.

[0010] More preferably, the weight ratio of the DHA algal oil to the salmon oil is (3-15):(5-12).

[0011] More preferably, the weight ratio of the DHA algal oil to the salmon oil is (8-15):(6-12).

[0012] More preferably, the weight ratio of the DHA algal oil to the salmon oil is 10:8.

[0013] Preferably, the DHA algal oil contains ≥40% DHA; more preferably, the DHA algal oil contains ≥50% DHA.

[0014] Preferably, the salmon oil contains ≥3.0% DHA and ≥3.0% EPA; more preferably, the salmon oil contains ≥3.9% DHA and ≥3.2% EPA.

[0015] Preferably, the functional ingredients include bird's nest peptides, bovine colostrum, mushroom powder, and fermented Alpinia oxyphylla powder; more preferably, the weight ratio of bird's nest peptides, bovine colostrum, mushroom powder, and fermented Alpinia oxyphylla powder in the functional ingredients is (0.01-0.08):(0.1-1.0):(0.5-1.0):(0.1-0.5).

[0016] More preferably, the weight ratio of the bird's nest peptide, bovine colostrum, mushroom powder, and fermented Alpinia oxyphylla powder is (0.03-0.08):(0.4-1.0):(0.6-1.0):(0.2-0.5).

[0017] More preferably, the weight ratio of the bird's nest peptide, bovine colostrum, mushroom powder, and fermented Alpinia oxyphylla powder is 0.05:0.6:0.75:0.3.

[0018] Preferably, the bird's nest peptide is a small molecule peptide extracted from bird's nest after pretreatment (crushing, soaking) and hydrolysis.

[0019] Preferably, the bovine colostrum contains 10%-20% immunoglobulins.

[0020] Preferably, the mushroom powder is prepared by selecting raw materials, cleaning, cutting, blanching, cooling and draining, and drying.

[0021] Preferably, the fermented Alpinia oxyphylla powder is obtained by drying the supernatant obtained after Alpinia oxyphylla is fermented by cellulase and pectinase followed by the addition of Lactobacillus plantarum and Lactobacillus rhamnosus.

[0022] Preferably, the weight ratio of Alpinia oxyphylla to cellulase is (3-6):1; the weight ratio of Alpinia oxyphylla to pectinase is (6-8):1; the weight ratio of Alpinia oxyphylla to Lactobacillus rhamnosus is (400-500):1; and the weight ratio of Alpinia oxyphylla to Lactobacillus plantarum is (700-800):1.

[0023] Further preferred preparation process of fermented Alpinia oxyphylla powder is as follows: (1) Alpinia oxyphylla and water are mixed, and cellulase and pectinase are added for enzymatic hydrolysis to obtain enzymatic hydrolysate; (2) The enzymatic hydrolysate is heated to inactivate enzymes, cooled and then fermented with Lactobacillus plantarum and Lactobacillus rhamnosus to obtain fermentation liquid; (3) The fermentation liquid is separated into solid and liquid, and the supernatant is dried to obtain fermented Alpinia oxyphylla powder.

[0024] More preferably, the ratio of the Alpinia oxyphylla powder to water is 1:(25-40).

[0025] More preferably, the enzymatic hydrolysis temperature is 50-60℃ and the time is 80-100 min; the temperature for inactivating the enzyme is 95-110℃ and the time is 55-65 min; and the fermentation temperature is 25-30℃ and the time is 20-30 h.

[0026] Preferably, the emulsifier includes glyceryl citrate, diacetyl tartaric acid mono- and diglycerides, and Tween 80.

[0027] The weight ratio of the citrate fatty acid glyceride, diacetyl tartaric acid mono- and diglycerides and Tween 80 is (0.5-1.0):(0.05-0.12):(0.02-0.06).

[0028] More preferably, the weight ratio of the citrate fatty acid glyceride, diacetyl tartaric acid mono- and diglycerides and Tween 80 is 0.7:0.09:0.035.

[0029] Preferably, the nanoemulsion for improving hyperactivity, anxiety and memory also includes fruit juice; specifically, it includes 0.1-0.15 parts by weight of the fruit juice.

[0030] More preferably, the juice includes one or more of apple concentrate, strawberry concentrate, and citrus concentrate; even more preferably, the juice in this invention is strawberry concentrate.

[0031] Preferably, the pH of the nanoemulsion is less than 5.0, and the pH is adjusted using a phosphate buffer solution.

[0032] More preferably, the phosphate buffer is a phosphate buffer (pH 5.0), which is prepared by taking a certain amount of 0.2 mol / L sodium dihydrogen phosphate solution and adjusting the pH value to 5.0 with sodium hydroxide test solution.

[0033] Preferably, the processing aid is glycerin, and the antioxidant is vitamin E.

[0034] More preferably, the glycerol is 2-4 parts by weight, and the vitamin E is 0.01-0.02 parts by weight.

[0035] Preferably, the present invention provides a nanoemulsion for improving anxiety and hyperactivity and enhancing memory, comprising, by weight, the following components: 10 parts DHA algal oil, 8 parts salmon oil, 0.7 parts citric acid fatty acid glycerides, 0.09 parts diacetyl tartaric acid mono- and diglycerides, 0.035 parts Tween 80, 3 parts glycerin, 0.05 parts bird's nest peptide, 0.6 parts bovine colostrum, 0.75 parts mushroom powder, 0.3 parts fermented Alpinia oxyphylla powder, 0.015 parts vitamin E, 0.12 parts strawberry concentrate, and 76.34 parts water.

[0036] On the other hand, the present invention provides a method for preparing a nanoemulsion that improves hyperactivity, anxiety, and enhances memory, comprising the following steps:

[0037] (1) Mix emulsifier, processing aid and water, heat and stir to fully dissolve each component to obtain crude emulsion A; (2) After crude emulsion A is cooled to room temperature, add oil and antioxidant to crude emulsion A, stir and mix evenly to obtain crude emulsion B; (3) Add functional ingredients and fruit juice to crude emulsion B, and shear to obtain emulsion C; (4) Adjust the pH of emulsion C to less than 5.0 to obtain emulsion D; (5) After high pressure homogenization of emulsion D, cool to room temperature to obtain nano emulsion.

[0038] Preferably, the heating and stirring temperature in step (1) is 60-70℃ and the time is 15-30min; more preferably, the temperature is 65℃ and the stirring time is 20min.

[0039] Preferably, the stirring time in step (2) is 20-40 min; more preferably, the stirring time is 30 min.

[0040] Preferably, the shearing speed in step (3) is 6500-8500 rpm and the shearing time is 15-30 min; more preferably, the speed is 7500 rpm and the shearing time is 20 min.

[0041] Preferably, the homogenization pressure in step (5) is 200-400 bar and the homogenization time is 20-100 min; more preferably, the homogenization pressure is 300 bar and the homogenization time is 60 min.

[0042] The above-mentioned nanoemulsions are used in the preparation of products that improve hyperactivity, anxiety, and enhance memory.

[0043] Compared with the prior art, the present invention has the following advantages:

[0044] 1. The nanoemulsion provided by this invention for improving hyperactivity, anxiety, and enhancing memory uses DHA algal oil and salmon oil as its main raw materials. DHA algal oil is a crucial source of high-purity DHA, offering advantages such as natural origin, safety, controllability, and lack of pollution, and it is virtually odorless. Salmon oil is not only rich in DHA but also contains a certain amount of EPA. Both are rich in various unsaturated fatty acids and active ingredients, which can synergistically enhance the effects of functional components, effectively improving symptoms of anxiety and hyperactivity while simultaneously improving memory.

[0045] 2. The nanoemulsion provided by this invention, which improves hyperactivity, anxiety, and enhances memory, is obtained by synergistically combining functional ingredients such as bird's nest peptide, bovine colostrum, mushroom powder, and fermented Alpinia oxyphylla powder with nutritional oils. Open field experiments have shown that it can improve hyperactivity in rats; water maze experiments have shown that it can improve memory in rats; and it can increase 5-HT levels in rats. Zebrafish anxiety model experiments have also shown that it can improve anxiety behavior in zebrafish.

[0046] 3. This invention provides a nanoemulsion that improves hyperactivity, anxiety, and memory. It has a pleasant taste, is suitable for children, and has a simple preparation method that is applicable to industrial production. Detailed Implementation

[0047] The following non-limiting embodiments are intended to enable those skilled in the art to gain a more comprehensive understanding of the present invention, but do not limit the invention in any way. The following content is merely an exemplary description of the scope of protection claimed by the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, and such changes should also fall within the scope of protection claimed by the present invention.

[0048] When numerical ranges are given in the embodiments, it should be understood that, unless otherwise stated in the invention, both endpoints of each numerical range and any value between the two endpoints may be selected. Unless otherwise defined, all technical and scientific terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0049] The present invention will be further described below by way of specific embodiments. Unless otherwise specified, all chemical reagents used in the embodiments of the present invention are obtained through conventional commercial channels. Products from different manufacturers do not have a significant impact on the effectiveness.

[0050] DHA algal oil: DHA content ≥50%, purchased from Qingdao Haizhiyuan Life Science Technology Co., Ltd.; Salmon oil: DHA content ≥3.9%, EPA content ≥3.2%, purchased from Noah Saint-Noah (Taicang) Biotechnology Co., Ltd.; Citric acid fatty acid glycerides: food grade, purchased from Shenzhen Antai Biotechnology Co., Ltd., item number 080; Diacetyl tartrate mono- and diglycerides: food grade, purchased from Guangzhou Jinqixin Chemical New Materials Co., Ltd., CAS No.: 977051-29-8; Tween 80: purchased from Guangdong Runhua Chemical Co., Ltd., model: T80; Bird's nest peptides: food grade, purchased from Hubei Ruibang Biotechnology Co., Ltd., item number 08; Bovine colostrum: food grade, containing 10-20% immunoglobulins, purchased from Guangzhou Tianwu Bioengineering Co., Ltd., item number: 038; Mushroom powder: food grade. Grade A, purchased from Xinghua Changhong Food Co., Ltd., item number a-04; Alpinia oxyphylla powder, purchased from Bozhou Bowantang Pharmaceutical Co., Ltd., item number 137; Vitamin E, purchased from Zhejiang Pharmaceutical Co., Ltd., CAS number: 59-02-09; Strawberry concentrate: purchased from Fujian Luquan Food Co., Ltd., model 49-52BX; Cellulase (enzyme activity 20,000 U / g) and pectinase (enzyme activity 30,000 U / g) were both purchased from Shandong Longkete Enzyme Preparation Co., Ltd.; Lactobacillus rhamnosus and Lactobacillus plantarum were both purchased from Jinqiao Biotechnology Co., Ltd. Lactobacillus rhamnosus F-1 (live count 300 billion CFU / g) and Lactobacillus plantarum LPL28 (live count 700 billion CFU / g); Flaxseed oil: α-linolenic acid content >50%, purchased from Qingdao Haizhiyuan Life Technology Co., Ltd.

[0051] Preparation example: Fermented Alpinia oxyphylla powder

[0052] (1) Mix 30 parts by weight of Alpinia oxyphylla powder with 1000 parts by weight of water to obtain a traditional Chinese medicine mixture; (2) Add sodium bicarbonate to the traditional Chinese medicine mixture to adjust the pH value to 5.6, then add 7 parts by weight of cellulase and 5 parts by weight of pectinase, and enzymatically hydrolyze at 55℃ for 90 min to obtain an enzymatic hydrolysate; (3) Heat the enzymatic hydrolysate to 100℃ and inactivate the enzyme for 60 min to obtain an enzyme-inactivated extract; (4) After the enzyme-inactivated extract is cooled to 30℃, add 0.07 parts by weight of Lactobacillus rhamnosus and 0.04 parts by weight of Lactobacillus plantarum, and ferment at 30℃ for 24 h to obtain a fermentation liquid; (5) Separate the solid and liquid of the fermentation liquid to obtain the upper fermentation clear liquid, and spray dry to obtain fermented Alpinia oxyphylla powder.

[0053] Example 1

[0054] This embodiment provides a nanoemulsion for improving hyperactivity, anxiety, and enhancing memory. By weight, it comprises 10 parts DHA algal oil, 8 parts salmon oil, 0.7 parts citric acid fatty acid glycerides, 0.09 parts diacetyl tartaric acid mono- and diglycerides, 0.035 parts Tween 80, 3 parts glycerol, 0.05 parts bird's nest peptide, 0.6 parts bovine colostrum, 0.75 parts mushroom powder, 0.3 parts fermented Alpinia oxyphylla powder, 0.015 parts vitamin E, 0.12 parts strawberry concentrate, and 76.34 parts water.

[0055] Its preparation method is as follows:

[0056] (1) Weighing and mixing: Weigh all materials according to the proportion and set aside; (2) Mix citric acid fatty acid glycerides, diacetyl tartaric acid mono- and diglycerides, Tween 80, glycerol and water, and stir at 65°C for 20 min until uniform to obtain crude emulsion A; (3) After crude emulsion A is cooled to room temperature, add DHA algal oil, salmon oil and vitamin E to crude emulsion A, stir for 30 min and mix evenly to obtain crude emulsion B; (4) Add bird's nest peptide, bovine colostrum, mushroom powder, fermented Alpinia oxyphylla powder and strawberry concentrate to crude emulsion B, and shear at 7500 rpm for 20 min to obtain emulsion C; (5) Add phosphate buffer solution to emulsion C to adjust pH to 4.5 to obtain emulsion D; (6) Homogenize emulsion D through a circulating high pressure homogenizer, and homogenize at 300 bar for 60 min, cool to room temperature to obtain nano emulsion.

[0057] Example 2

[0058] This embodiment provides a nanoemulsion for improving hyperactivity, anxiety, and enhancing memory. By weight, it comprises 3 parts DHA algal oil, 12 parts salmon oil, 0.5 parts citric acid fatty acid glycerides, 0.05 parts diacetyl tartaric acid mono- and diglycerides, 0.03 parts Tween 80, 2 parts glycerol, 0.01 parts bird's nest peptide, 0.1 parts bovine colostrum, 0.5 parts mushroom powder, 0.1 parts fermented Alpinia oxyphylla powder, 0.01 parts vitamin E, 0.1 parts strawberry concentrate, and 81.60 parts water.

[0059] Its preparation method is as follows:

[0060] (1) Weighing and mixing: Weigh all materials according to the proportion and set aside; (2) Mix citric acid fatty acid glycerides, diacetyl tartaric acid mono- and diglycerides, Tween 80, glycerol and water, and stir at 60℃ for 30 min until uniform to obtain crude emulsion A; (3) After crude emulsion A is cooled to room temperature, add DHA algal oil, salmon oil and vitamin E to crude emulsion A, stir for 35 min and mix evenly to obtain crude emulsion B; (4) Add bird's nest peptide, bovine colostrum, mushroom powder, fermented Alpinia oxyphylla powder and strawberry concentrate to crude emulsion B, and shear at 7000 rpm for 15 min to obtain emulsion C; (5) Add phosphate buffer solution to emulsion C to adjust pH to 4.5 to obtain emulsion D; (6) Homogenize emulsion D through a circulating high pressure homogenizer, and homogenize at 200 bar for 100 min, cool to room temperature to obtain nano emulsion.

[0061] Example 3

[0062] This embodiment provides a nanoemulsion for improving hyperactivity, anxiety, and enhancing memory. By weight, it comprises 15 parts DHA algal oil, 6 parts salmon oil, 1.0 part citric acid fatty acid glyceride, 0.08 parts diacetyl tartaric acid mono- and diglycerides, 0.06 parts Tween 80, 4 parts glycerol, 0.03 parts bird's nest peptide, 0.4 parts bovine colostrum, 0.6 parts mushroom powder, 0.5 parts fermented Alpinia oxyphylla powder, 0.018 parts vitamin E, 0.15 parts strawberry concentrate, and 72.162 parts water.

[0063] Its preparation method is as follows:

[0064] (1) Weighing and mixing: Weigh all materials according to the proportion and set aside; (2) Mix citric acid fatty acid glycerides, diacetyl tartaric acid mono- and diglycerides, Tween 80, glycerol and water, and stir at 63°C for 15 min until uniform to obtain crude emulsion A; (3) After crude emulsion A is cooled to room temperature, add DHA algal oil, salmon oil and vitamin E to crude emulsion A, stir for 40 min and mix evenly to obtain crude emulsion B; (4) Add bird's nest peptide, bovine colostrum, mushroom powder, fermented Alpinia oxyphylla powder and strawberry concentrate to crude emulsion B, and shear at 6500 rpm for 30 min to obtain emulsion C; (5) Add phosphate buffer solution to emulsion C to adjust pH to 4.5 to obtain emulsion D; (6) Homogenize emulsion D through a circulating high pressure homogenizer, and homogenize at 400 bar for 20 min, cool to room temperature to obtain nano emulsion.

[0065] Example 4

[0066] This embodiment provides a nanoemulsion for improving hyperactivity, anxiety, and enhancing memory. By weight, it comprises 8 parts DHA algal oil, 5 parts salmon oil, 0.55 parts citric acid fatty acid glycerides, 0.12 parts diacetyl tartaric acid mono- and diglycerides, 0.02 parts Tween 80, 3 parts glycerol, 0.08 parts bird's nest peptide, 1.0 part bovine colostrum, 1.0 part mushroom powder, 0.2 parts fermented Alpinia oxyphylla powder, 0.02 parts vitamin E, 0.14 parts strawberry concentrate, and 80.87 parts water.

[0067] Its preparation method is as follows:

[0068] (1) Weighing and mixing: Weigh all materials according to the proportion and set aside; (2) Mix citric acid fatty acid glycerides, diacetyl tartaric acid mono- and diglycerides, Tween 80, glycerol and water, and stir at 70℃ for 25 min until uniform to obtain crude emulsion A; (3) After crude emulsion A is cooled to room temperature, add DHA algal oil, salmon oil and vitamin E to crude emulsion A, stir for 20 min and mix evenly to obtain crude emulsion B; (4) Add bird's nest peptide, bovine colostrum, mushroom powder, fermented Alpinia oxyphylla powder and strawberry concentrate to crude emulsion B, and shear at 8500 rpm for 25 min to obtain emulsion C; (5) Add phosphate buffer solution to emulsion C to adjust pH to 4.5 to obtain emulsion D; (6) Homogenize emulsion D through a circulating high pressure homogenizer, and homogenize at 350 bar for 40 min, cool to room temperature to obtain nano emulsion.

[0069] Comparative Example 1

[0070] This comparative example provides a nanoemulsion for improving hyperactivity, anxiety, and memory. The only difference between this example and Example 1 is that it does not include salmon oil. Instead, it includes 18 parts by weight of DHA algal oil, 0.7 parts by weight of citric acid fatty acid glycerides, 0.09 parts by weight of diacetyl tartaric acid mono- and diglycerides, 0.035 parts by weight of Tween 80, 3 parts by weight of glycerin, 0.05 parts by weight of bird's nest peptide, 0.6 parts by weight of bovine colostrum, 0.75 parts by weight of mushroom powder, 0.3 parts by weight of fermented Alpinia oxyphylla powder, 0.015 parts by weight of vitamin E, 0.12 parts by weight of strawberry concentrate, and 76.34 parts by weight of water.

[0071] Its preparation method is the same as that in Example 1.

[0072] Comparative Example 2

[0073] This comparative example provides a nanoemulsion for improving hyperactivity, anxiety, and enhancing memory. The only difference from Example 1 is that it does not include DHA algal oil. Instead, it comprises 18 parts by weight of salmon oil, 0.7 parts by weight of citric acid fatty acid glycerides, 0.09 parts by weight of diacetyl tartaric acid mono- and diglycerides, 0.035 parts by weight of Tween 80, 3 parts by weight of glycerin, 0.05 parts by weight of bird's nest peptides, 0.6 parts by weight of bovine colostrum, 0.75 parts by weight of mushroom powder, 0.3 parts by weight of fermented Alpinia oxyphylla powder, 0.015 parts by weight of vitamin E, 0.12 parts by weight of strawberry concentrate, and 76.34 parts by weight of water.

[0074] Its preparation method is the same as that in Example 1.

[0075] Comparative Example 3

[0076] This comparative example provides a nanoemulsion for improving hyperactivity, anxiety, and enhancing memory. The only difference from Example 1 is that it does not include bird's nest peptides. Instead, it includes 10 parts by weight of DHA algal oil, 8 parts by weight of salmon oil, 0.7 parts by weight of citric acid fatty acid glycerides, 0.09 parts by weight of diacetyl tartaric acid mono- and diglycerides, 0.035 parts by weight of Tween 80, 3 parts by weight of glycerol, 0.6 parts by weight of bovine colostrum, 0.75 parts by weight of mushroom powder, 0.3 parts by weight of fermented Alpinia oxyphylla powder (the total weight of bovine colostrum, mushroom powder, and fermented Alpinia oxyphylla powder is the same as the total weight of bird's nest peptides, bovine colostrum, mushroom powder, and fermented Alpinia oxyphylla powder in Example 1), 0.015 parts by weight of vitamin E, 0.12 parts by weight of strawberry concentrate, and 76.34 parts by weight of water.

[0077] Its preparation method is the same as that in Example 1.

[0078] Comparative Example 4

[0079] This comparative example provides a nanoemulsion for improving hyperactivity, anxiety, and memory. The only difference from Example 1 is that it does not include bovine colostrum. Instead, it includes 10 parts by weight of DHA algal oil, 8 parts by weight of salmon oil, 0.7 parts by weight of citric acid fatty acid glycerides, 0.09 parts by weight of diacetyl tartaric acid mono- and diglycerides, 0.035 parts by weight of Tween 80, 3 parts by weight of glycerol, 0.05 parts by weight of bird's nest peptide, 0.75 parts by weight of mushroom powder, 0.3 parts by weight of fermented Alpinia oxyphylla powder (the total weight of bird's nest peptide, mushroom powder, and fermented Alpinia oxyphylla powder is the same as the total weight of bird's nest peptide, bovine colostrum, mushroom powder, and fermented Alpinia oxyphylla powder in Example 1), 0.015 parts by weight of vitamin E, 0.12 parts by weight of strawberry concentrate, and 76.34 parts by weight of water.

[0080] Its preparation method is the same as that in Example 1.

[0081] Comparative Example 5

[0082] This comparative example provides a nanoemulsion for improving hyperactivity, anxiety, and enhancing memory. The only difference from Example 1 is that it does not include mushroom powder. Instead, it comprises 10 parts by weight of DHA algal oil, 8 parts by weight of salmon oil, 0.7 parts by weight of citric acid fatty acid glycerides, 0.09 parts by weight of diacetyl tartaric acid mono- and diglycerides, 0.035 parts by weight of Tween 80, 3 parts by weight of glycerol, 0.05 parts by weight of bird's nest peptide, 0.6 parts by weight of bovine colostrum, 0.3 parts by weight of fermented Alpinia oxyphylla powder (the total weight of bird's nest peptide, bovine colostrum, and fermented Alpinia oxyphylla powder is the same as the total weight of bird's nest peptide, bovine colostrum, mushroom powder, and fermented Alpinia oxyphylla powder in Example 1), 0.015 parts by weight of vitamin E, 0.12 parts by weight of strawberry concentrate, and 76.34 parts by weight of water.

[0083] Its preparation method is the same as that in Example 1.

[0084] Comparative Example 6

[0085] This comparative example provides a nanoemulsion for improving hyperactivity, anxiety, and enhancing memory. The only difference from Example 1 is that it does not include fermented Alpinia oxyphylla powder. Instead, it comprises 10 parts by weight of DHA algal oil, 8 parts by weight of salmon oil, 0.7 parts by weight of citric acid fatty acid glycerides, 0.09 parts by weight of diacetyl tartaric acid mono- and diglycerides, 0.035 parts by weight of Tween 80, 3 parts by weight of glycerol, 0.05 parts by weight of bird's nest peptide, 0.6 parts by weight of bovine colostrum, 0.75 parts by weight of mushroom powder (the total weight of bird's nest peptide, bovine colostrum, and mushroom powder is the same as the total weight of bird's nest peptide, bovine colostrum, mushroom powder, and fermented Alpinia oxyphylla powder in Example 1), 0.015 parts by weight of vitamin E, 0.12 parts by weight of strawberry concentrate, and 76.34 parts by weight of water.

[0086] Its preparation method is the same as that in Example 1.

[0087] Comparative Example 7

[0088] This comparative example provides a nanoemulsion for improving hyperactivity, anxiety, and enhancing memory. The only difference from Example 1 is that salmon oil is replaced with flaxseed oil. The nanoemulsion comprises 10 parts by weight of DHA algal oil, 8 parts by weight of flaxseed oil, 0.7 parts by weight of citric acid fatty acid glycerides, 0.09 parts by weight of diacetyl tartaric acid mono- and diglycerides, 0.035 parts by weight of Tween 80, 3 parts by weight of glycerol, 0.05 parts by weight of bird's nest peptide, 0.6 parts by weight of bovine colostrum, 0.75 parts by weight of mushroom powder, 0.3 parts by weight of fermented Alpinia oxyphylla powder, 0.015 parts by weight of vitamin E, 0.12 parts by weight of strawberry concentrate, and 76.34 parts by weight of water.

[0089] Its preparation method is the same as that in Example 1.

[0090] Comparative Example 8

[0091] This comparative example provides a nanoemulsion for improving hyperactivity, anxiety, and enhancing memory. The only difference from Example 1 is that it does not include diacetyl tartrate mono- and diglycerides. Instead, it comprises 10 parts by weight of DHA algal oil, 8 parts by weight of salmon oil, 0.79 parts by weight of citrate fatty acid glycerides, 0.035 parts by weight of Tween 80, 3 parts by weight of glycerol, 0.05 parts by weight of bird's nest peptide, 0.6 parts by weight of bovine colostrum, 0.75 parts by weight of mushroom powder, 0.3 parts by weight of fermented Alpinia oxyphylla powder, 0.015 parts by weight of vitamin E, 0.12 parts by weight of strawberry concentrate, and 76.34 parts by weight of water.

[0092] Its preparation method is the same as that in Example 1.

[0093] Experimental Example 1: Determination of Particle Size and Stability of Nanoemulsions

[0094] 1.1 Determination of average particle size and polydispersity index (PDI) of nanoemulsions

[0095] The nanoemulsions of Examples 1-4 and Comparative Examples 1-8 were dispensed into 50 mL glass bottles, with three parallel groups per group, and allowed to stand at room temperature (25°C) for 15 days. After 15 days, 5 mL of the nanoemulsion samples from Examples 1-4 and Comparative Examples 1-8 were placed in a constant temperature water bath (70°C) and diluted 500 times with 70°C deionized water, then cooled to room temperature. The diluted samples were added to the sample cell of the nanoparticle analyzer, with the sample volume not less than 2 / 3. The sample cell was then inserted into the sample slot, and the nanoparticle analyzer's chamber lid was closed. The particle size and PDI were measured. Each sample was measured three times, and the average value was taken. The results are shown in Table 1.

[0096] Table 1. Average particle size and PDI determination results of nanoemulsions

[0097]

[0098] Note: Compared with Example 1, +P<0.05, ++P<0.01.

[0099] Results analysis:

[0100] As shown in Table 1, the nanoemulsions prepared in Examples 1-4 have lower particle size and PDI values, indicating that the prepared nanoemulsions have better stability. In particular, the nanoemulsion prepared in Example 1 has a smaller average particle size and PDI value, and better stability. The larger the nanoemulsion particle size, the larger its PDI value, and the worse the stability of the nanoemulsion. Among them, the nanoemulsion prepared in Comparative Example 8 showed a significant increase in particle size and PDI value after 15 days of storage, indicating that the combination of citric acid fatty acid glycerides, diacetyl tartaric acid mono- and diglycerides, and Tween 80 as emulsifiers and other components can better achieve the stability of the nanoemulsion system.

[0101] Experiment Example 2: Rat Experiment

[0102] To verify that the nanoemulsion of this invention has the effect of improving hyperactivity and enhancing memory, the following animal experiments are described:

[0103] Lead acetate: 99% purity, purchased from Hebei Mojin Biotechnology Co., Ltd., CAS No.: 301-04-2; Rat 5-HT (5-HT) ELISA kit (catalog number: SP12579) was purchased from Wuhan Saipei Biotechnology Co., Ltd.

[0104] The open field test chamber (model: XR-XZ 301) and the Morris water maze (model: XR-XM101) were both purchased from Shanghai Xinruan Information Technology Co., Ltd.; the enzyme-linked immunosorbent assay (ELISA) analyzer was purchased from Shenzhen Leidu Life Science Co., Ltd., model: RT-6100.

[0105] Preparation of lead acetate solution: Dissolve 0.2g of lead acetate in 50 mL of distilled water, stir to dissolve, and pour into a 1000 mL volumetric flask. Rinse the beaker with distilled water while pouring the washing solution into the 1000 mL volumetric flask. Repeat 2-3 times, and make up to 1000 mL with distilled water.

[0106] Animals: Four-week-old SPF-grade male SD rats, weighing (60±10) g.

[0107] Rats were fed standard feed and drinking water at a temperature of 20-26℃ and humidity of 40%-70%, with alternating light and dark periods of 12 hours each, and free access to food and water for 3 days for acclimatization. The rats were then divided into three groups: one group for the open field test, one for the water maze test, and one for neurotransmitter measurement in the rat brain; each group was randomly divided into 13 subgroups (n=10 per subgroup): normal group, model group, Examples 1-4, and Comparative Examples 1-7. All animals were fed standard feed and had free access to water under uniform conditions. At the start of the experiment, the normal group had free access to purified water, while the model group, Examples 1-4, and Comparative Examples 1-7 had free access to a 0.2 mg / mL lead acetate solution to establish the model. Simultaneously, Examples 1-4 and Comparative Examples 1-7 were administered the corresponding nanoemulsion by gavage at the same time each day, while the model group and normal group were administered an equal volume of physiological saline once daily for 4 weeks.

[0108] The administration regimen was to administer the medication by gavage once a day at a fixed time. Based on the human body's daily dose of 120 mL, the gavage dose for rats was calculated to be 10.8 mL / kg using the equivalent dose conversion method based on body surface area ratio. This dose was administered by gavage.

[0109] 2.1 Behavioral testing of rats

[0110] 2.1.1 Open Field Experiment

[0111] Four weeks after gavage, rats underwent an open field test. An open field test chamber (50cm x 50cm x 40cm) was used to assess the rats' behavior and spontaneous activity. The test chamber had a 3x3 grid design. Before the test, the rats were brought to the laboratory to familiarize themselves with the environment. At the start of the test, the rats were placed in the center and quickly moved away, the curtain was drawn, and another experimenter simultaneously pressed the start button on the monitoring system to observe the rats' activity within the open field chamber for 5 minutes, monitoring the resting time, movement time, and movement distance. After each rat experiment, the test chamber was thoroughly cleaned, sprayed with 75% alcohol, and dried to eliminate odor and prevent interference with the next animal.

[0112] 2.1.2 Morris Water Maze Experiment

[0113] Three weeks after gavage, a water maze test was conducted on the rats. The Morris water maze video analysis system was used to evaluate the rats' learning and memory abilities. The rats completed learning and memory training in a water tank (1.6 m in diameter) with the water temperature maintained at (25±1)℃. The water was dyed black with added ink. The tank was divided into four quadrants, with a platform placed in the second quadrant. On day 1, the rats underwent an adaptive swimming exercise for 60 seconds. From day 2 to day 5, the rats underwent a hidden platform space experiment. Four times a day, the rats were randomly placed into the maze from the four quadrants. The actual time taken to climb onto the platform within 120 seconds was recorded. If the rats did not find the platform, 120 seconds was recorded, and the rats were guided to climb onto the platform. On day 6, the platform was removed, and the rats underwent a space exploration experiment. The percentage of distance traveled to the target quadrant, the percentage of time taken, and the number of times the rats entered the target quadrant within 120 seconds were recorded.

[0114] 2.2 Determination of neurotransmitters in the rat brain

[0115] Twenty-four hours after the last administration, rats were decapitated and placed on ice to collect brain tissue. The brain tissue was homogenized into a 10% homogenate using ice-cold saline in an ice bath. This homogenate was then centrifuged at 4°C and 2500 rpm for 20 minutes in a low-temperature high-speed centrifuge. The supernatant was collected and placed on ice for later use. The 5-hydroxytryptamine (5-HT) content was detected according to the assay kit instructions. The absorbance was measured at 450 nm using an ELISA reader to calculate the sample concentration.

[0116] 2.3 Experimental Results

[0117] 2.3.1 Results of the open field experiment

[0118] Table 2 Results of open field experiments in each group of rats

[0119]

[0120] Note: In the table, if there is only one letter in the same column, the difference is not significant; if there are different letters in the same column, the difference is significant (P<0.05).

[0121] Results analysis:

[0122] A hyperactivity disorder (ADHD) model was established using lead acetate, with the open field test used to observe the voluntary movement ability of the experimental animals. According to the results in Table 2, compared with the normal group, the model group rats showed a decrease in rest time and an increase in movement time and total movement distance in the open field test after modeling, and the differences were statistically significant (P<0.05), indicating that the hyperactivity ability of the model group rats was enhanced, demonstrating successful modeling.

[0123] Compared with the model group, the resting time of rats in Examples 1-4 was significantly prolonged, while the movement time and total movement distance were significantly reduced, indicating that the nanoemulsion provided by the present invention can improve the hyperactive behavior of rats.

[0124] The difference between Comparative Examples 1-2 and 7 and Example 1 is that they use different oils. As shown in Table 2, compared with Example 1, after rats were fed the nanoemulsion, the resting time of Comparative Examples 1-2 and 7 decreased to varying degrees, while the movement time and total movement distance increased to varying degrees, and the differences were significant. This indicates that the DHA algal oil and salmon oil provided by the present invention can better improve the hyperactive behavior of rats.

[0125] Compared with Example 1, in Comparative Examples 3-6, rats fed with nanoemulsion showed a significant decrease in rest time and a significant increase in movement time and total movement distance, indicating that the functional ingredients affect the hyperactive behavior of rats. Therefore, the functional ingredients of bird's nest peptide, bovine colostrum, mushroom powder and fermented Alpinia oxyphylla powder provided by the present invention, when used in combination with nutritional oils, can better improve the hyperactive behavior of rats.

[0126] 2.3.2 Results of the Water Maze Experiment

[0127] Table 3 Results of the water maze experiment in each group of rats

[0128]

[0129] Note: In the table, if there is only one letter in the same column, the difference is not significant; if there are different letters in the same column, the difference is significant (P<0.05).

[0130] Results analysis:

[0131] The water maze test is used to assess the spatial learning and memory abilities of animals. As shown in Table 3, compared with the model group, Examples 1-4 exhibited varying degrees of increase in the target quadrant movement time ratio, target quadrant run ratio, and number of entries into the target quadrant, with significant differences. This indicates that the nanoemulsion prepared in this invention can improve the learning and memory abilities of rats.

[0132] The target quadrant movement time ratio, target quadrant run ratio, and number of times entering the target quadrant of rats in Comparative Examples 1-2 and 7 were all lower than those in Example 1, indicating that nutritional oils can affect the learning and memory abilities of rats. The synergistic effect of DHA algal oil and salmon oil in this invention can effectively improve the memory ability of rats.

[0133] Compared with Example 1, Comparative Examples 3-6 showed varying degrees of reduction in the target quadrant movement time ratio, target quadrant run ratio, and number of times entering the target quadrant, indicating that the functional components and nutrient oils provided by this invention can better improve the learning and memory abilities of rats.

[0134] 2.3.3 Results of Neurotransmitter Level Experiments

[0135] Table 4. Results of neurotransmitter level detection in rat brain

[0136]

[0137] Note: In the table, if there is only one letter in the same column, the difference is not significant; if there are different letters in the same column, the difference is significant (P<0.05).

[0138] Results analysis:

[0139] 5-Hydroxytryptamine (5-HT) is a regulator of various functions of the nervous system and can regulate human mood. It has been shown to be closely related to depression, anxiety, and other conditions. In Examples 1-4, compared with the model group, the 5-HT content in the rat brain tissue increased to varying degrees, indicating that the nanoemulsion prepared in this invention can affect the 5-HT content in the rat brain, thereby improving symptoms of anxiety and hyperactivity.

[0140] Compared with Example 1, Comparative Examples 1-7 showed varying degrees of reduction in 5-HT content in rat brain tissue, which was significant. This indicates that the synergistic effect of the nutritional oils and functional components provided by the present invention can better improve the 5-HT content in the rat brain, thereby improving anxiety and hyperactivity.

[0141] Experiment Example 3: Zebrafish Experiment

[0142] To verify that the nanoemulsion of this invention has the effect of improving anxiety, the following animal experiments are described:

[0143] 3.1 Reproduction of Zebrafish Juveniles

[0144] Wild-caught AB-type zebrafish, aged 12 months, were cultured at approximately 26°C and fed daily with brine shrimp. The night before spawning, they were transferred to 2L spawning tanks, with two males and two females separated by a partition. At 7:30 AM the following day, the partition was removed, and 30 minutes later, the spawned eggs were aspirated and sterilized with 100mg / L methylene blue before being transferred to 90mm culture dishes (50 eggs / dish). The water was changed on the second night and the third morning, and dead eggs were removed. Fry began to hatch after 72 hours and were fed shrimp paste. Once all the fish had hatched, the fry were transferred to fish tanks (50 fish / tank) and fed and had their water changed morning and evening. The light cycle was 14 hours of light / 10 hours of dark.

[0145] 3.2 Monitoring the movement distance of zebrafish

[0146] Zebrafish 30 days post-fertilization were divided into a control group, a model group, Examples 1-4, and Comparative Examples 1-7. In 90mm culture dishes, the control group was cultured in embryonic water, the model group in 0.1 nmol / L 1-(3-chlorophenyl)piperazine hydrochloride (mCPP), and Examples 1-4 and Comparative Examples 1-7 in a nanoemulsion containing 0.1 nmol / L mCPP (dilution ratio 1:100) for 30 min. Zebrafish of uniform size were then selected from each group and placed in 12-well plates. After acclimatization for 6 min, they were immediately placed in a ZebraLab analysis system for 10 min of tracking and recording of their swimming distance and trajectory. The ZebraLab system recorded data every 20 seconds for a total of 10 min. Ten zebrafish were placed in each group.

[0147] 3.3 Experimental Results

[0148] Table 5. Movement distance of zebrafish 30 days after fertilization

[0149]

[0150] Note: Compared with the blank group, △P<0.05, △△P<0.01; compared with the model group, +P<0.05, ++P<0.01; compared with Example 1, #P<0.05, ##P<0.01.

[0151] Results analysis:

[0152] This study investigated the therapeutic effect of nanoemulsions on anxiety induced by 1-(3-chlorophenyl)piperazine hydrochloride (mCPP) in zebrafish. Compared with the control group, the model group showed a significant increase in movement distance, demonstrating that the zebrafish were in an anxiety state and the model was successfully established.

[0153] Compared with the model group, the zebrafish movement distance in Examples 1-4 decreased to varying degrees, indicating that the nanoemulsion prepared in this invention can affect the movement distance of zebrafish, thereby improving anxiety symptoms.

[0154] Compared with Example 1, Comparative Examples 1-7 showed varying degrees of increase in the movement distance of zebrafish, which was significant, indicating that the synergistic effect of the nutritional oils and functional ingredients provided by the present invention can better improve anxiety behavior.

[0155] Experiment Example 4: Taste Evaluation

[0156] The taste of the nanoemulsions of Examples 1-4 was evaluated.

[0157] Experimental Methods: Twenty volunteers were recruited, with requirements including: age 19-30 years, sensitive taste and smell, clear sensory description, no recent history of colds, fevers, oral inflammation, or allergies. All 20 volunteers consumed the nanoemulsions prepared in Examples 1-4 of this invention, and their taste evaluations were recorded. The taste evaluation criteria for the nanoemulsions are shown in Table 6.

[0158] Data Processing: Individual Score: Remove the highest and lowest scores from all individual scores, and calculate using the following formula.

[0159]

[0160] The taste evaluation results of Examples 1-4 are shown in Table 7.

[0161] Table 6. Taste Evaluation Criteria for Nanoemulsions

[0162]

[0163] Table 7. Taste Evaluation Results of Nanoemulsions

[0164]

[0165] As shown in Tables 6 and 7, for the nanoemulsions prepared in Examples 1-4, the total scores of the 20 volunteers in terms of color, texture, and taste were all above 37 points, which is very good. This indicates that the nanoemulsions prepared in Examples 1-4 of this invention have a good taste.

[0166] In summary, the nanoemulsion provided by this invention can improve anxiety and hyperactivity and enhance memory by influencing hyperactivity behavior, learning and memory ability, neurotransmitter levels, and zebrafish movement distance in rats.

[0167] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A nanoemulsion for improving hyperactivity, anxiety, and enhancing memory, characterized in that, By weight, it comprises the following components: 8-27 parts of nutritional oils, 0.57-1.18 parts of emulsifiers, 2-4 parts of processing aids, 0.71-2.58 parts of functional ingredients, 0.01-0.02 parts of antioxidants, and 70-85 parts of water; the nutritional oils include DHA algal oil and salmon oil. The weight ratio of DHA algal oil to salmon oil is (3-15):(5-12). The functional ingredients include bird's nest peptides, bovine colostrum, mushroom powder, and fermented Alpinia oxyphylla powder; the weight ratio of bird's nest peptides, bovine colostrum, mushroom powder, and fermented Alpinia oxyphylla powder in the functional ingredients is (0.01-0.08):(0.1-1.0):(0.5-1.0):(0.1-0.5). The bird's nest peptide is a small molecule peptide extracted from bird's nest after it has been crushed, soaked, and hydrolyzed; the manufacturer is Hubei Ruibang Biotechnology Co., Ltd., and the product number is 08. The bovine colostrum contains 10%-20% immunoglobulins; The mushroom powder is prepared by selecting raw materials, cleaning, cutting, blanching, cooling and draining, and drying; the manufacturer is Xinghua Changhong Food Co., Ltd., and the product number is a-04; The fermented Alpinia oxyphylla powder is obtained by drying the supernatant after Alpinia oxyphylla has been fermented by cellulase and pectinase, followed by the addition of Lactobacillus plantarum and Lactobacillus rhamnosus for solid-liquid separation. The emulsifier includes glyceryl citrate, diacetyl tartaric acid mono- and diglycerides, and Tween 80; the weight ratio of glyceryl citrate, diacetyl tartaric acid mono- and diglycerides, and Tween 80 in the emulsifier is (0.5-1.0):(0.05-0.12):(0.02-0.06).

2. The nanoemulsion for improving hyperactivity, anxiety, and memory according to claim 1, wherein The weight ratio of Alpinia oxyphylla to cellulase is (3-6):1; the weight ratio of Alpinia oxyphylla to pectinase is (6-8):1; the weight ratio of Alpinia oxyphylla to Lactobacillus rhamnosus is (400-500):1; and the weight ratio of Alpinia oxyphylla to Lactobacillus plantarum is (700-800):

1.

3. The nanoemulsion for improving hyperactivity, anxiety, and memory according to claim 1, wherein The preparation process of the fermented Alpinia oxyphylla powder is as follows: (1) Alpinia oxyphylla and water are mixed, and cellulase and pectinase are added for enzymatic hydrolysis to obtain an enzymatic hydrolysate; (2) The enzymatic hydrolysate is heated to inactivate the enzyme, cooled and then fermented with Lactobacillus plantarum and Lactobacillus rhamnosus to obtain a fermentation liquid; (3) The fermentation liquid is separated into solid and liquid, and the supernatant is dried to obtain fermented Alpinia oxyphylla powder.

4. The nanoemulsion for improving hyperactivity, anxiety, improving memory according to claim 3, characterized by, The ratio of the Alpinia oxyphylla powder to water is 1:(25-40).

5. The nanoemulsion for improving hyperactivity, anxiety, improving memory according to claim 3, wherein The enzymatic hydrolysis is performed at a temperature of 50-60℃ for 80-100 min; the enzyme inactivation is performed at a temperature of 95-110℃ for 55-65 min; and the fermentation is performed at a temperature of 25-30℃ for 20-30 h.

6. The nanoemulsion for improving hyperactivity, anxiety, improving memory according to claim 1, wherein, By weight, the processing aid includes 2-4 parts glycerin, the antioxidant includes 0.01-0.02 parts vitamin E, and also includes 0.1-0.15 parts fruit juice.

7. The nanoemulsion for improving hyperactivity, anxiety, and enhancing memory according to claim 6, characterized in that, By weight, it includes the following ingredients: 10 parts DHA algal oil, 8 parts salmon oil, 0.7 parts citric acid fatty acid glycerides, 0.09 parts diacetyl tartaric acid mono- and diglycerides, 0.035 parts Tween 80, 3 parts glycerin, 0.05 parts bird's nest peptide, 0.6 parts bovine colostrum, 0.75 parts mushroom powder, 0.3 parts fermented Alpinia oxyphylla powder, 0.015 parts vitamin E, 0.12 parts strawberry concentrate, and 76.34 parts water.

8. A method for preparing the nanoemulsion for improving hyperactivity, anxiety, and enhancing memory as described in any one of claims 1-7, characterized in that, Includes the following steps: (1) Mix emulsifier, processing aid and water, heat and stir to fully dissolve each component to obtain crude emulsion A; (2) After crude emulsion A is cooled to room temperature, add oil and antioxidant to crude emulsion A, stir and mix evenly to obtain crude emulsion B; (3) Add functional ingredients and fruit juice to crude emulsion B, and shear to obtain emulsion C; (4) Adjust the pH of emulsion C to less than 5.0 to obtain emulsion D; (5) After high pressure homogenization of emulsion D, cool to room temperature to obtain nano emulsion.

9. The method for preparing the nanoemulsion for improving hyperactivity, anxiety, and enhancing memory as described in claim 8, characterized in that, The heating and stirring temperature in step (1) is 60-70℃, and the time is 15-30 min; The stirring time in step (2) is 20-40 min; In step (3), the shearing speed is 6500-8500 rpm and the shearing time is 15-30 min; In step (5), the homogenization pressure is 200-400 bar and the homogenization time is 20-100 min.

10. The application of the nanoemulsion according to any one of claims 1-7 and the nanoemulsion obtained by the preparation method according to any one of claims 8-9 in the preparation of products for improving hyperactivity, anxiety and enhancing memory.

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