Nutrient injection for fruit tree trunk and preparation method thereof
Patent Information
- Application Number
- CN202511058939.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-30
- Publication Date
- 2026-08-21
- Estimated Expiration
- 2045-07-30
AI Technical Summary
[0003]现有技术中,营养注射液通常适用于移栽后果树的快速营养供给,然而直接注入枝干的营养液对果树的根系营养供给效果不足,使得移栽后的果树无法快速的恢复生长状态,影响了果树移栽的生活率
[0021]1、本发明中,在果树树干用营养注射液的制备过程中,通过银杏叶、迷迭香和荷叶的组合添加,其中,利用银杏叶的银杏内酯成分可促进果树根系细胞的分裂与伸长,使得果树的根系更加发达,增强果树在移栽后对水分和养分的吸收能力,迷迭香酸具有调节植物激素平衡的作用,能刺激果树在移栽后的根系受损位置再次生根,提高根系的活力和吸收效率,荷叶碱成分有助于改善果树根系的生长环境,促进果树根系的微生物活动,为果树根系生长提供良好的条件,进而有效提升了果树的产量以及移栽成活率。
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Abstract
Description
Technical Field
[0001] This invention relates to the field of nutrient injection technology, specifically to a nutrient injection for fruit tree trunks and its preparation method. Background Technology
[0002] Fruit tree trunk nutrient injection is a preparation that supplements fruit trees with nutrients. It is injected into the trunk through a special needle, allowing the fruit tree to absorb it directly and quickly replenish nutrients.
[0003] In existing technologies, nutrient injection solutions are generally suitable for rapid nutrient supply to transplanted fruit trees. However, nutrient solutions injected directly into the branches and trunks are insufficient for root nutrition, preventing transplanted fruit trees from quickly recovering their growth and affecting their survival rate. Therefore, this invention provides a nutrient injection solution for fruit tree trunks and its preparation method. Summary of the Invention
[0004] The purpose of this invention is to provide a nutrient injection solution for fruit tree trunks and its preparation method. The nutrient injection solution for fruit tree trunks prepared by this invention not only has good yield-increasing performance, but also excellent survival rate performance, effectively improving the performance of the nutrient injection solution.
[0005] To achieve the above objectives, the present invention provides the following technical solution:
[0006] In a first aspect, the present invention provides a nutrient injection solution for fruit tree trunks, comprising the following raw materials: urea, compound nutrient agent, additive solution, sucrose, and water, wherein the weight percentage of each raw material is: 4-6% urea, 8-10% compound nutrient agent, 6-10% additive solution, 4-6% sucrose, and the remainder is supplemented by water to 100%.
[0007] The preparation of the additive solution includes the following steps:
[0008] S1: Preparation of the mixture. The raw materials of the mixture include ginkgo leaves, rosemary, lotus leaves and water. Before the preparation of the mixture, the ginkgo leaves, rosemary and lotus leaves are pretreated to obtain component B material and extract.
[0009] S2: Culture medium preparation, the raw materials of which include malt extract, wheat bran, glucose and agar;
[0010] S3: Fermentation treatment, the mixture and culture medium are fermented with microbial agents to obtain the additive solution.
[0011] Further, the pretreatment method for the ginkgo leaves, rosemary, and lotus leaves is as follows: After washing and drying, the ginkgo leaves, rosemary, and lotus leaves are placed in an oven at 40-50°C for 2-4 hours. Afterward, they are pulverized to a particle size of 60-100 μm to obtain coarse material. This coarse material is divided into component A and component B. Component A and an ethanol-water solution are added to a water bath at 50-60°C, and a magnetic stirrer is connected to the bath. The stirring speed was set to 200-300 r / min, and the mixture was kept at a constant temperature for 2-4 hours. The resulting product was then added to a centrifuge and centrifuged at 4000-6000 r / min for 6-10 minutes. The supernatant was obtained after centrifugation and filtered to obtain the extract. This completed the pretreatment of ginkgo leaves, rosemary, and lotus leaves. The mass ratio of component A material to ethanol aqueous solution was 1:(4-6), and the mass concentration of ethanol aqueous solution was 60-70%.
[0012] Furthermore, the mass ratio of component A to component B is 1:(0.1-0.2), and the mass ratio of extract to additive is 1:(2-3).
[0013] Furthermore, the method for preparing the mixture is as follows: the extract and water are added to a mixer, the mixer is set to 300-400 r / min and stirred for 10-20 min, and the resulting product is subjected to high-temperature sterilization to obtain the mixture.
[0014] Further, the method for preparing the culture medium is as follows: malt extract and 2 to 4 times its weight of water are added to a pot and boiled for 16 to 20 minutes. After cooling to room temperature, the mixture is added to a water bath. Component B, wheat bran, glucose, and agar are added to the water bath. The water bath temperature is set to 50 to 60°C. A magnetic stirrer is connected and set to a speed of 200 to 300 r / min. The mixture is stirred at a constant temperature for 20 to 30 minutes. The resulting product is then subjected to high-temperature sterilization to obtain the culture medium.
[0015] Further, the fermentation treatment method is as follows: the mixture and culture medium are mixed and added to an incubator, the incubator is added with bacterial agent and supplement, the incubator is placed in a constant temperature shaker, the constant temperature shaker is set to 28-32℃, 180-200r / min, and cultured for 4-6h to obtain a culture medium, the culture medium is added to a refrigerated centrifuge, and centrifuged at 2-4℃, 4000-6000r / min for 10-20min to obtain a supernatant, the supernatant is filtered to complete the fermentation treatment and obtain the additive solution.
[0016] Furthermore, the mass of the microbial agent is 0.06-0.08% of the mass of the mixture, the mass of the supplement is 1-3% of the mass of the mixture, and the microbial agent is a mixture of Paecilomyces lilacinus and actinomycetes of equal mass.
[0017] Furthermore, the supplement is prepared by mixing indoleacetic acid, nano-chitosan, vitamin C, sodium naphthaleneacetate and deionized water, with the mass ratio of each raw material being 1:(0.6-0.8):(1.4-1.6):(0.6-0.8):(2-4).
[0018] Furthermore, the compound nutrient is prepared by mixing ferrous sulfate, zinc sulfate, manganese sulfate, potassium nitrate, calcium nitrate, potassium hydroxide, potassium dihydrogen phosphate, humic acid and water, with the mass ratio of each raw material being 1:(0.4-0.6):(0.2-0.4):1:(0.1-0.3):(0.1-0.3):(0.2-0.4):(0.8-1.2):(6-8).
[0019] Secondly, the present invention also provides a method for preparing a nutrient injection for fruit tree trunks, comprising the following steps: weighing urea, compound nutrient, additive liquid, sucrose and water as needed and adding them to a mixer, setting the mixer to 600-800 r / min and stirring for 20-30 min to obtain the nutrient injection for fruit tree trunks.
[0020] Compared with the prior art, the beneficial effects of the present invention are:
[0021] 1. In the preparation of the nutrient injection solution for fruit tree trunks in this invention, ginkgo leaves, rosemary, and lotus leaves are added in combination. Among them, the ginkgolide component of ginkgo leaves can promote the division and elongation of root cells in fruit trees, making the root system of fruit trees more developed and enhancing the ability of fruit trees to absorb water and nutrients after transplanting. Rosmarinic acid has the effect of regulating the balance of plant hormones, which can stimulate the fruit trees to re-root at the damaged root sites after transplanting, improve the vitality and absorption efficiency of the root system. The lotus leaf alkaloid component helps to improve the growth environment of the fruit tree root system, promote the microbial activity of the fruit tree root system, and provide good conditions for the growth of the fruit tree root system, thereby effectively improving the yield of fruit trees and the survival rate of transplanting.
[0022] 2. In this invention, during the preparation of the nutrient injection solution for fruit tree trunks, the culture medium provides nutrients and energy, and the extract is fermented using a microbial agent. After fermentation, *Paecilomyces lilacinus* secretes plant growth hormones, which promote root development. The actinomycetes produce antibiotics, inhibiting pathogenic microorganisms, and simultaneously decompose organic matter to release nutrients, which has a good growth recovery effect on transplanted fruit trees. Indoleacetic acid and sodium naphthaleneacetate can directly stimulate plant cell division and root formation in fruit trees. At the same time, the large amount of antioxidants in the fermentation products can reduce oxidative damage during fruit tree transplantation, effectively improving the survival rate of transplanted fruit trees. Attached Figure Description
[0023] Figure 1The invention presents a flowchart of a nutrient injection solution for fruit tree trunks and its preparation method. Detailed Implementation
[0024] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.
[0025] It should be noted that the raw materials used in the following embodiments are all commercially available.
[0026] Example 1: Composed of the following raw materials: urea, compound nutrient, additive solution, sucrose, and water. The weight percentage of each raw material is: 4% urea, 8% compound nutrient, 6% additive solution, 4% sucrose, and the remainder is made up to 100% by water.
[0027] The preparation of the additive solution includes the following steps:
[0028] S1: Preparation of the mixture. The raw materials of the mixture include ginkgo leaves, rosemary, lotus leaves and water. Before the preparation of the mixture, the ginkgo leaves, rosemary and lotus leaves are pretreated to obtain component B material and extract.
[0029] The pretreatment method for ginkgo leaves, rosemary, and lotus leaves is as follows: After washing and drying, the ginkgo leaves, rosemary, and lotus leaves are placed in an oven at 40℃ for 2 hours. After drying, they are pulverized to a particle size of 60μm to obtain coarse material. The coarse material is divided into component A and component B. Component A and an ethanol aqueous solution are added to a water bath at 50℃ and connected to a magnetic stirrer at 200r / min. The mixture is stirred at a constant temperature for 2 hours. The resulting product is then added to a centrifuge at 4000r / min for 6 minutes. After centrifugation, the supernatant is obtained and filtered to obtain the extract. This completes the pretreatment of ginkgo leaves, rosemary, and lotus leaves. The mass ratio of component A to ethanol aqueous solution is 1:4, the mass concentration of ethanol aqueous solution is 60%, the mass ratio of component A to component B is 1:0.1, and the mass ratio of extract to additive solution is 1:2.
[0030] The method for preparing the mixture is as follows: the extract and water are added to a mixer, the mixer is set to 300 r / min and stirred for 10 min, and the resulting product is subjected to high-temperature sterilization to obtain the mixture;
[0031] S2: Culture medium preparation, the raw materials of which include malt extract, wheat bran, glucose and agar;
[0032] The method for preparing the culture medium is as follows: malt extract and twice its weight of water are added to a pot and boiled for 16 minutes. After cooling to room temperature, the mixture is added to a water bath. Component B, wheat bran, glucose, and agar are added to the water bath. The water bath temperature is set to 50°C. A magnetic stirrer is connected and set to a speed of 200 r / min. The mixture is stirred at a constant temperature for 20 minutes. The resulting product is then subjected to high-temperature sterilization to obtain the culture medium.
[0033] S3: Fermentation treatment, the mixture and culture medium are fermented with microbial agents to obtain the additive solution;
[0034] The fermentation process is as follows: the mixture and culture medium are added to an incubator, where microbial agents and supplements are added. The incubator is placed in a constant-temperature shaker, which is set to 28℃ and 180 r / min for 4 hours to obtain a culture medium. The culture medium is then added to a refrigerated centrifuge and centrifuged at 2℃ and 4000 r / min for 10 minutes to obtain a supernatant. The supernatant is filtered to complete the fermentation process and obtain an additive solution. The microbial agent accounts for 0.06% of the mass of the mixture, and the supplement accounts for 1% of the mass of the mixture. The microbial agent is a mixture of Paecilomyces lilacinus and actinomycetes of equal mass. The supplement is prepared by mixing indoleacetic acid, nano-chitosan, vitamin C, sodium naphthaleneacetate, and deionized water in a mass ratio of 1:0.6:1.4:0.6:2.
[0035] The compound nutrient solution is prepared by mixing ferrous sulfate, zinc sulfate, manganese sulfate, potassium nitrate, calcium nitrate, potassium hydroxide, potassium dihydrogen phosphate, fulvic acid and water, with the mass ratio of each raw material being 1:0.4:0.2:1:0.1:0.1:0.2:0.8:6.
[0036] The preparation method of nutrient injection for fruit tree trunks includes the following steps: weigh out urea, compound nutrient, additive solution, sucrose and water as needed and add them to a mixer. Set the mixer to 600 r / min and stir for 20 min to obtain nutrient injection for fruit tree trunks.
[0037] Example 2: Composed of the following raw materials: urea, compound nutrient, additive solution, sucrose, and water. The weight percentage of each raw material is: 5% urea, 9% compound nutrient, 8% additive solution, 5% sucrose, and the remainder is made up to 100% by water.
[0038] The preparation of the additive solution includes the following steps:
[0039] S1: Preparation of the mixture. The raw materials of the mixture include ginkgo leaves, rosemary, lotus leaves and water. Before the preparation of the mixture, the ginkgo leaves, rosemary and lotus leaves are pretreated to obtain component B material and extract.
[0040] The pretreatment method for ginkgo leaves, rosemary, and lotus leaves is as follows: After washing and drying, the ginkgo leaves, rosemary, and lotus leaves are placed in an oven at 45℃ for 3 hours. After drying, they are pulverized to a particle size of 80μm to obtain coarse material. The coarse material is divided into component A and component B. Component A and ethanol aqueous solution are added to a water bath at 55℃ and connected to a magnetic stirrer at 250r / min. The mixture is stirred at a constant temperature for 3 hours. The resulting product is then added to a centrifuge at 5000r / min for 8 minutes. After centrifugation, the supernatant is obtained and filtered to obtain the extract. This completes the pretreatment of ginkgo leaves, rosemary, and lotus leaves. The mass ratio of component A to ethanol aqueous solution is 1:5, the mass concentration of ethanol aqueous solution is 65%, the mass ratio of component A to component B is 1:0.15, and the mass ratio of extract to additive solution is 1:2.5.
[0041] The method for preparing the mixture is as follows: the extract and water are added to a mixer, the mixer is set to 350 r / min and stirred for 15 min, and the resulting product is subjected to high-temperature sterilization to obtain the mixture;
[0042] S2: Culture medium preparation, the raw materials of which include malt extract, wheat bran, glucose and agar;
[0043] The method for preparing the culture medium is as follows: malt extract and three times its weight of water are added to a pot and boiled for 18 minutes. After cooling to room temperature, the mixture is added to a water bath. Component B, wheat bran, glucose, and agar are added to the water bath. The water bath temperature is set to 55°C. A magnetic stirrer is connected and set to a speed of 250 r / min. The mixture is stirred at a constant temperature for 25 minutes. The resulting product is then subjected to high-temperature sterilization to obtain the culture medium.
[0044] S3: Fermentation treatment, the mixture and culture medium are fermented with microbial agents to obtain the additive solution;
[0045] The fermentation process is as follows: the mixture and culture medium are added to an incubator, where microbial agents and supplements are added. The incubator is placed in a constant-temperature shaker, which is set to 30℃ and 190 r / min for 5 hours to obtain a culture solution. The culture solution is then added to a refrigerated centrifuge and centrifuged at 3℃ and 5000 r / min for 15 minutes to obtain the supernatant. The supernatant is then filtered to complete the fermentation process and obtain the additive solution. The microbial agent accounts for 0.07% of the mass of the mixture, and the supplement accounts for 2% of the mass of the mixture. The microbial agent is a mixture of Paecilomyces lilacinus and actinomycetes of equal mass. The supplement is prepared by mixing indoleacetic acid, nano-chitosan, vitamin C, sodium naphthaleneacetate, and deionized water in a mass ratio of 1:0.7:1.5:0.7:3.
[0046] The compound nutrient solution is prepared by mixing ferrous sulfate, zinc sulfate, manganese sulfate, potassium nitrate, calcium nitrate, potassium hydroxide, potassium dihydrogen phosphate, humic acid and water, with the mass ratio of each raw material being 1:0.5:0.3:1:0.2:0.2:0.3:1:7.
[0047] The preparation method of nutrient injection for fruit tree trunks includes the following steps: weigh out urea, compound nutrient, additive solution, sucrose and water as needed and add them to a mixer. Set the mixer to 700 r / min and stir for 25 min to obtain nutrient injection for fruit tree trunks.
[0048] Example 3: Composed of the following raw materials: urea, compound nutrient, additive solution, sucrose, and water. The weight percentage of each raw material is: 6% urea, 10% compound nutrient, 10% additive solution, 6% sucrose, and the remainder is made up to 100% by water.
[0049] The preparation of the additive solution includes the following steps:
[0050] S1: Preparation of the mixture. The raw materials of the mixture include ginkgo leaves, rosemary, lotus leaves and water. Before the preparation of the mixture, the ginkgo leaves, rosemary and lotus leaves are pretreated to obtain component B material and extract.
[0051] The pretreatment method for ginkgo leaves, rosemary, and lotus leaves is as follows: After washing and drying, the ginkgo leaves, rosemary, and lotus leaves are placed in an oven at 50°C for 4 hours. Afterward, they are pulverized to a particle size of 100 μm to obtain coarse material. The coarse material is divided into component A and component B. Component A and an ethanol aqueous solution are added to a water bath at 60°C, and a magnetic stirrer is connected at 300 r / min. The mixture is stirred at this constant temperature for 4 hours. The resulting product is then added to a centrifuge at 6000 r / min for 10 minutes. After centrifugation, the supernatant is obtained and filtered to obtain the extract. This completes the pretreatment of ginkgo leaves, rosemary, and lotus leaves. The mass ratio of component A to the ethanol aqueous solution is 1:6, the mass concentration of the ethanol aqueous solution is 70%, the mass ratio of component A to component B is 1:0.2, and the mass ratio of the extract to the additive solution is 1:3.
[0052] The method for preparing the mixture is as follows: the extract and water are added to a mixer, the mixer is set to 400 r / min and stirred for 20 min, and the resulting product is subjected to high-temperature sterilization to obtain the mixture;
[0053] S2: Culture medium preparation, the raw materials of which include malt extract, wheat bran, glucose and agar;
[0054] The method for preparing the culture medium is as follows: malt extract and 4 times its weight of water are added to a pot and boiled for 20 minutes. After cooling to room temperature, it is added to a water bath. Component B, wheat bran, glucose, and agar are added to the water bath. The water bath temperature is set to 60°C. A magnetic stirrer is connected and set to a speed of 300 r / min. The mixture is stirred at a constant temperature for 30 minutes. The resulting product is then subjected to high-temperature sterilization to obtain the culture medium.
[0055] S3: Fermentation treatment, the mixture and culture medium are fermented with microbial agents to obtain the additive solution;
[0056] The fermentation process is as follows: the mixture and culture medium are added to an incubator, where microbial agents and supplements are added. The incubator is placed in a constant-temperature shaker, which is set to 32℃ and 200 rpm for 6 hours to obtain a culture broth. The culture broth is then added to a refrigerated centrifuge and centrifuged at 4℃ and 6000 rpm for 20 minutes to obtain the supernatant. The supernatant is then filtered to complete the fermentation process and obtain the additive solution. The microbial agent accounts for 0.08% of the mass of the mixture, and the supplement accounts for 3% of the mass of the mixture. The microbial agent is a mixture of Paecilomyces lilacinus and actinomycetes of equal mass. The supplement is prepared by mixing indoleacetic acid, nano-chitosan, vitamin C, sodium naphthaleneacetate, and deionized water in a mass ratio of 1:0.8:1.6:0.8:4.
[0057] The compound nutrient solution is prepared by mixing ferrous sulfate, zinc sulfate, manganese sulfate, potassium nitrate, calcium nitrate, potassium hydroxide, potassium dihydrogen phosphate, humic acid and water, with the mass ratio of each raw material being 1:0.6:0.4:1:0.3:0.3:0.4:1.2:8.
[0058] The preparation method of nutrient injection for fruit tree trunks includes the following steps: weigh out urea, compound nutrient, additive solution, sucrose and water as needed and add them to a mixer. Set the mixer to 800 r / min and stir for 30 min to obtain nutrient injection for fruit tree trunks.
[0059] Comparative Example 1: The difference between this comparative example and Example 1 is that an equal amount of indoleacetic acid was used as a substitute for the supplement in this comparative example.
[0060] Comparative Example 2 differs from Example 1 in that it does not contain any supplements.
[0061] Comparative Example 3 differs from Example 1 in that an equal amount of malt extract is used to replace the additive liquid in this comparative example.
[0062] Comparative Example 4 differs from Example 1 in that it does not contain any additives.
[0063] Performance testing: The performance of the nutrient injection solutions used to prepare fruit tree trunks in Examples 1, 2, 3, 1, 2, 3, and 4 was tested, and the test data are recorded in the table below:
[0064]
[0065] In the performance test, the trunks of the fruit trees prepared in Examples 1, 2, 3, 1, 2, 3 and 4 were injected with nutrient injection solution into the transplanted apple trees in batches. The survival rate of the apple trees was recorded 100 days after transplanting. Each batch of apple trees consisted of 100 trees. The yield of each batch of apple trees was recorded within one planting cycle after transplanting, and the average yield per tree was calculated.
[0066] It is evident that the yield performance and survival rate of the fruit tree trunk nutrient injections prepared in Comparative Examples 1, 2, 3, and 4 are all lower than those in Examples 1, 2, and 3. This indicates that during the preparation of the fruit tree trunk nutrient injection, the combined addition of ginkgo leaves, rosemary, and lotus leaves has several advantages. Ginkgolide from ginkgo leaves can promote the division and elongation of root cells in fruit trees, resulting in a more developed root system and enhanced absorption of water and nutrients after transplanting. Rosmarinic acid plays a role in regulating plant hormone balance, stimulating the re-rooting of damaged roots after transplanting, and improving root vitality and absorption efficiency. The alkaloid component of lotus leaves helps improve the growth environment of fruit tree roots, promotes microbial activity in the roots, and provides favorable conditions for root growth, thereby effectively increasing the yield and transplant survival rate of fruit trees.
[0067] In the preparation of nutrient injection solution for fruit tree trunks, the culture medium provides nutrients and energy, and the extract is fermented with the help of microbial agents. After fermentation, Paecilomyces lilacinus secretes plant growth hormones, which promotes root development. Actinomycetes produce antibiotics to inhibit pathogenic microorganisms and decompose organic matter to release nutrients, which has a good effect on the growth recovery of transplanted fruit trees. Indoleacetic acid and sodium naphthaleneacetate can directly stimulate plant cell division and root formation of fruit trees. At the same time, the large amount of antioxidants in the fermentation products can reduce oxidative damage during fruit tree transplantation and effectively improve the survival rate of transplanted fruit trees.
[0068] By comparing and analyzing the relevant data in the table, it can be seen that the nutrient injection for fruit tree trunks prepared by this invention not only has good yield-increasing performance but also excellent survival rate performance. This indicates that the nutrient injection for fruit tree trunks provided by this invention has a broader market prospect and is more suitable for widespread application.
[0069] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.
[0070] The preferred embodiments of the present invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims
1. A nutrient injection solution for fruit tree trunks, characterized in that: It consists of the following raw materials: urea, compound nutrient, additive solution, sucrose, and water. The weight percentage of each raw material is: 4-6% urea, 8-10% compound nutrient, 6-10% additive solution, and 4-6% sucrose, with the remainder made up to 100% by water. The preparation of the additive solution includes the following steps: S1: Preparation of mixture: The extract and water are added to a mixer and mixed. The resulting product is then sterilized at high temperature to obtain the mixture. The extract was prepared by pretreatment of ginkgo leaves, rosemary, and lotus leaves. The pretreatment method was as follows: Ginkgo leaves, rosemary and lotus leaves were washed, dried and then placed in an oven for drying. After that, they were crushed to obtain coarse material. The coarse material was divided into component A and component B. Component A and ethanol aqueous solution were added to a water bath and stirred at a constant temperature. The resulting product was centrifuged to obtain the supernatant. The supernatant was filtered to obtain the extract. S2: Culture medium preparation: malt extract and water are added to a pot and boiled. After cooling to room temperature, the mixture is added to a water bath. Component B, bran, glucose, and agar are added to the water bath. The mixture is stirred at a constant temperature. The resulting product is then sterilized at high temperature to obtain the culture medium. S3: Fermentation treatment: After mixing the mixture and culture medium, add them to the incubator. Add the inoculum and supplement to the incubator. Place the incubator in a constant temperature shaker and incubate for 4-6 hours to obtain the culture medium. Add the culture medium to a refrigerated centrifuge for centrifugation and obtain the supernatant. Filter the supernatant to complete the fermentation treatment and obtain the additive solution. The supplement is prepared by mixing indoleacetic acid, nano-chitosan, vitamin C, sodium naphthaleneacetate and deionized water, with the mass ratio of each raw material being 1:(0.6-0.8):(1.4-1.6):(0.6-0.8):(2-4). The inoculant is a mixture of equal masses of Paecilomyces lilacinus and actinomycetes; The compound nutrient is prepared by mixing ferrous sulfate, zinc sulfate, manganese sulfate, potassium nitrate, calcium nitrate, potassium hydroxide, potassium dihydrogen phosphate, humic acid and water, with the mass ratio of each raw material being 1:(0.4-0.6):(0.2-0.4):1:(0.1-0.3):(0.1-0.3):(0.2-0.4):(0.8-1.2):(6-8).
2. The nutrient injection solution for fruit tree trunks according to claim 1, characterized in that, The pretreatment method for ginkgo leaves, rosemary, and lotus leaves is as follows: After washing and drying, the ginkgo leaves, rosemary, and lotus leaves are placed in an oven at 40-50℃ for 2-4 hours. Afterward, they are pulverized to a particle size of 60-100 μm to obtain coarse material. This coarse material is divided into component A and component B. Component A and an ethanol-water solution are added to a water bath at 50-60℃, with a magnetic stirrer connected at a speed of 200-300 r / min. The mixture is stirred at this constant temperature for 2-4 hours. The resulting product is then added to a centrifuge at 4000-6000 r / min for 6-10 minutes. After centrifugation, the supernatant is obtained and filtered to obtain an extract, thus completing the pretreatment of ginkgo leaves, rosemary, and lotus leaves. The mass ratio of component A to the ethanol-water solution is 1:(4-6), and the mass concentration of the ethanol-water solution is 60-70%.
3. The nutrient injection solution for fruit tree trunks according to claim 2, characterized in that, The mass ratio of component A to component B is 1:(0.1 to 0.2).
4. The nutrient injection solution for fruit tree trunks according to claim 1, characterized in that, The method for preparing the mixture is as follows: the extract and water are added to a mixer, the mixer is set to 300-400 r / min and stirred for 10-20 min, and the resulting product is subjected to high-temperature sterilization to obtain the mixture.
5. The nutrient injection solution for fruit tree trunks according to claim 1, characterized in that, The method for preparing the culture medium is as follows: malt extract and 2-4 times its weight of water are added to a pot and boiled for 16-20 minutes. After cooling to room temperature, the mixture is added to a water bath. Component B, wheat bran, glucose, and agar are added to the water bath. The water bath temperature is set to 50-60°C. A magnetic stirrer is connected and set to a speed of 200-300 r / min. The mixture is stirred at a constant temperature for 20-30 minutes. The resulting product is then subjected to high-temperature sterilization to obtain the culture medium.
6. The nutrient injection solution for fruit tree trunks according to claim 1, characterized in that, The fermentation process is as follows: the mixture and culture medium are added to an incubator, and the incubator is filled with bacterial agent and supplement. The incubator is placed in a constant temperature shaker, which is set to 28-32°C and 180-200 r / min for 4-6 hours to obtain a culture solution. The culture solution is then added to a refrigerated centrifuge and centrifuged at 2-4°C and 4000-6000 r / min for 10-20 minutes to obtain a supernatant. The supernatant is then filtered to complete the fermentation process and obtain the additive solution.
7. The nutrient injection solution for fruit tree trunks according to claim 6, characterized in that, The mass of the microbial agent is 0.06-0.08% of the mass of the mixture, and the mass of the supplement is 1-3% of the mass of the mixture.
8. A method for preparing a nutrient injection solution for fruit tree trunks according to any one of claims 1 to 7, characterized in that, The process includes the following steps: Weigh out urea, compound nutrient, additive solution, sucrose and water as needed and add them to a mixer. Set the mixer to 600-800 r / min and stir for 20-30 minutes to obtain a nutrient injection solution for fruit tree trunks.
Citation Information
Patent Citations
Nutrient solution for transplanting trees
CN106588264A
Materials and Methods for Delivering a Substance to a Plant
US20200037607A1