Nucleic acid amplification detection module and device

Abstract

The application relates to a nucleic acid amplification detection module and device. The nucleic acid amplification detection module comprises a temperature adjusting mechanism and a fluorescence detection mechanism. The temperature adjusting mechanism is used for adjusting the temperature of a carrier so that the nucleic acid in the reaction sample in the carrier is amplified. The fluorescence detection mechanism is arranged opposite to the temperature adjusting mechanism with a spacing. The fluorescence detection mechanism is used for detecting the reaction sample after nucleic acid amplification. In use, the carrier to be subjected to fluorescence detection is placed between the temperature adjusting mechanism and the fluorescence detection mechanism. The temperature adjusting mechanism raises and lowers the temperature of the carrier so that the reaction sample in the carrier is subjected to reciprocating operation such as temperature rising and lowering, thereby realizing nucleic acid amplification. The fluorescence detection mechanism performs fluorescence detection on the nucleic acid amplification product in the carrier.

Description

Technical Field

[0001] This application relates to the field of in vitro diagnostic technology, and in particular to a nucleic acid amplification detection module and device. Background Technology

[0002] PCR (Polymerase Chain Reaction) is a molecular biology experimental method for the in vitro enzymatic synthesis of specific DNA fragments. It mainly consists of repeated thermal cycles of three steps: high-temperature denaturation, low-temperature annealing, and optimal extension. Before PCR amplification, the reaction sample needs to be placed in a vector. This sample comprises collected samples such as nasopharyngeal swabs, sputum, urine, feces, cervical / vaginal swabs, blood, cerebrospinal fluid, skin, and wound swabs, as well as reagents used for PCR amplification. During PCR amplification, the reaction sample is heated by a heater and cooled by a cooling mechanism, thus cycling the reaction sample through the high-temperature denaturation, low-temperature annealing, and optimal extension stages. Related nucleic acid amplification detection devices include a nucleic acid amplification detection module and a controller. The nucleic acid amplification detection module is electrically connected to the controller, and under the control of the controller, the nucleic acid amplification detection module completes amplification and optical detection.

[0003] However, in related nucleic acid amplification detection devices, miniaturized nucleic acid amplification modules have long amplification times, while those with slightly shorter amplification times are too bulky for large-scale deployment and use. Furthermore, the complex structure of nucleic acid amplification detection modules requires assembly by professionals, resulting in low assembly efficiency and high labor costs. Additionally, the need for specialized testing personnel to prepare reaction samples is inconvenient. Moreover, in rapid PCR, the accuracy and uniformity of temperature significantly impact amplification results. Rapid temperature control requires avoiding temperature overshoot that could affect reagent melting and extension, while temperature uniformity affects the consistency of amplification between wells or channels. However, existing PCR instruments cannot meet the temperature detection requirements for rapid PCR amplification, leading to inaccurate and biased fluorescence detection results.

[0004] Therefore, there is currently a lack of rapid PCR instruments and equipment that are easy to produce, easy to use, small in size, flexible in layout, and provide accurate fluorescence detection results. Summary of the Invention

[0005] To solve at least one of the above-mentioned technical problems, this application provides a nucleic acid amplification detection module and device, and the technical solution adopted is as follows.

[0006] On the one hand, this application provides a nucleic acid amplification detection module, including:

[0007] A temperature control mechanism for regulating the temperature of the carrier to amplify the nucleic acid of the reaction sample within the carrier; and

[0008] A fluorescence detection mechanism is provided, which is arranged at an interval relative to the temperature control mechanism. The fluorescence detection mechanism is used to detect the reaction sample after nucleic acid amplification.

[0009] In one embodiment, the nucleic acid amplification detection module further includes a support base located on the side of the temperature regulation mechanism facing the fluorescence detection mechanism, and the support base has a slot for inserting the carrier.

[0010] In one embodiment, the slot extends through the support along the arrangement direction of the fluorescence detection mechanism and the temperature regulation mechanism.

[0011] In one embodiment, the top of the slot is provided with a boss protruding toward the fluorescence detection mechanism on the side near the temperature regulation mechanism; the distance between the bottom surface of the boss and the bottom wall of the slot is greater than or equal to the length of the carrier.

[0012] In one embodiment, the top of the boss is provided with a first guide surface, and the distance between the first guide surface and the temperature adjustment mechanism increases along the insertion direction of the carrier; and / or, the bottom of the boss is provided with a second guide surface, and the distance between the second guide surface and the temperature adjustment mechanism increases along the removal direction of the carrier.

[0013] In one embodiment, the nucleic acid amplification detection module further includes a pressing mechanism for pressing the carrier against the temperature regulating mechanism or releasing the carrier along the arrangement direction.

[0014] In one embodiment, the nucleic acid amplification detection module further includes a first power mechanism, which is connected to the clamping mechanism and provides power to move the clamping mechanism to clamp or release the carrier; the clamping mechanism can avoid the fluorescence detection mechanism along its direction of movement.

[0015] In one embodiment, the nucleic acid amplification detection module further includes a first support member; the fluorescence detection mechanism is connected to the first support member; and the clamping mechanism is connected to the first support member.

[0016] In one embodiment, the first power mechanism includes a motor; the clamping mechanism includes:

[0017] Guide member, the guide member being connected to the first support member;

[0018] A movable frame, which is slidably disposed on the guide member;

[0019] A clamping element is connected to the movable frame, and the movable frame can drive the clamping element to move, so that the clamping element clamps the carrier or releases the carrier;

[0020] Nut, the nut being connected to the movable frame; and

[0021] A lead screw, which passes through the nut, and is connected to the shaft of the motor.

[0022] In one embodiment, the clamping mechanism further includes a coupling mounted on the first support member, the lead screw being coaxially connected to the coupling, and the coupling being connected to the shaft of the motor.

[0023] In one embodiment, one end of the guide is connected to the first support, and the other end of the guide is detachably connected to the temperature regulating mechanism or the support base.

[0024] In one embodiment, the clamping member includes a pressure head; the pressure head is made of a heat-insulating material and is used to clamp or loosen the carrier.

[0025] In one embodiment, the clamping member further includes a support plate connected between the movable frame and the pressure head.

[0026] In one embodiment, the support plate comprises a metal plate and / or a rigid non-metallic material plate.

[0027] In one embodiment, the nucleic acid amplification detection module further includes a second support member, which is connected between the fluorescence detection mechanism and the first support member; the movable frame is provided with an avoidance opening to avoid the second support member, and the second support member passes through the avoidance opening.

[0028] In one embodiment, the movable frame includes a movable plate and a third support member; the nut is connected to the movable plate, the movable plate is slidably disposed on the guide member, the movable plate and the first support member are spaced apart from each other, and the fluorescence detection mechanism is located on the side of the movable plate opposite to the first support member; the third support member is connected between the clamping member and the movable plate.

[0029] In one embodiment, the nucleic acid amplification detection module further includes a housing, the housing having a window and a cover plate slidably disposed at the window, the window being positioned opposite to the slot.

[0030] In one embodiment, the nucleic acid amplification detection module further includes a second power mechanism connected to the cover plate, which drives the cover plate to slide to open or close the window.

[0031] In one embodiment, the nucleic acid amplification detection module further includes a mounting frame connected to the housing, and the first power mechanism and the second power mechanism are mounted on the mounting frame.

[0032] In one embodiment, the mounting bracket is detachably connected to the first support member.

[0033] In one embodiment, the housing is disposed on top of the temperature regulation mechanism and the fluorescence detection mechanism; the nucleic acid amplification detection module further includes a light shield, which is connected to the housing, the first controller or the mounting bracket, and is disposed outside the fluorescence detection mechanism.

[0034] In one embodiment, the temperature regulating mechanism includes a heater for contacting the carrier to raise the temperature of the carrier. The heater includes a support layer, a main heating element, and a heat spreader plate. The main heating element is connected to the support layer, and the heat spreader plate is connected to the support layer.

[0035] In one embodiment, the heater further includes an auxiliary heating element connected to the temperature distribution plate.

[0036] In one embodiment, there are multiple auxiliary heating elements, which are respectively disposed at multiple different parts of the heat spreader.

[0037] In one embodiment, the main heating element includes a main heating coil; and / or, the auxiliary heating element includes an auxiliary heating coil.

[0038] In one embodiment, the main heating element is located between the support layer and the heat spreader; the auxiliary heating element is located on the side of the heat spreader opposite to the support layer.

[0039] In one embodiment, the heater further includes a first lead and a second lead, the first lead being electrically connected to the auxiliary heating element and used to connect to a voltage testing circuit; the second lead being electrically connected to the auxiliary heating element and used to connect to a current supply circuit.

[0040] In one embodiment, the material of the support layer includes a composite board of epoxy resin and glass fiber, ceramic, polyethylene terephthalate and / or glass.

[0041] In one embodiment, the temperature regulating mechanism further includes a radiator connected to the heater, the radiator being used to reduce the temperature of the heater.

[0042] In one embodiment, the temperature regulating mechanism further includes a heat-conducting element connected between the heater and the radiator.

[0043] In one embodiment, the temperature regulating mechanism further includes: a first circuit board and a support plate, wherein the first circuit board is electrically connected to the heater and the support plate is connected to the heater.

[0044] In one embodiment, the first circuit board is provided with a first clearance portion, the support plate is provided with a second clearance portion, the support plate is provided with a second clearance portion that is connected to the first clearance portion, and the heat-conducting component passes through the second clearance portion and the first clearance portion in sequence and is connected to the heat sink.

[0045] In one embodiment, the heat-conducting element has a protrusion that passes through the second clearance portion and the first clearance portion in sequence and is connected to the radiator; the support plate has a heat insulation component on the side near the heater; the heat insulation component is disposed between the heat-conducting element and the support plate.

[0046] In one embodiment, the nucleic acid amplification detection module further includes a first controller, and the temperature regulation mechanism, the fluorescence detection mechanism, the first power mechanism and the second power mechanism are all electrically connected to the first controller.

[0047] In one embodiment, the nucleic acid amplification detection module further includes a first circuit board, a second circuit board, and a third circuit board. The first circuit board is provided with a first electrical connector, and the second circuit board is provided with a second electrical connector. The first controller is provided with a third electrical connector and a fourth electrical connector. The first electrical connector is plugged into the third electrical connector, and the second electrical connector is plugged into the fourth electrical connector.

[0048] The third circuit board is provided with a fifth electrical connection connector, the first controller is provided with a sixth electrical connection connector, the first power mechanism and the second power mechanism are both electrically connected to the fifth electrical connection connector, and the fifth electrical connection connector and the sixth electrical connection connector are plugged into each other.

[0049] In one embodiment, the fluorescence detection mechanism is configured as a plurality of units; the plurality of fluorescence detection mechanisms are arranged sequentially.

[0050] On the other hand, this application provides a nucleic acid amplification detection device, including the aforementioned nucleic acid amplification detection module, and also includes a housing, wherein the housing is provided with a mounting part, and the nucleic acid amplification detection module is mounted on the mounting part.

[0051] In the aforementioned nucleic acid amplification detection module and device, the carrier to be detected for fluorescence is placed in the gap between the temperature control mechanism and the fluorescence detection mechanism. The temperature control mechanism raises and lowers the temperature of the carrier, causing the reaction sample within the carrier to undergo repeated heating and cooling cycles, thereby achieving nucleic acid amplification. The fluorescence detection mechanism then performs fluorescence detection on the nucleic acid amplification products within the carrier. Attached Figure Description

[0052] Figure 1 This is a structural diagram of a nucleic acid amplification and detection device according to an embodiment of this application.

[0053] Figure 2 for Figure 1 The exploded structure diagram shown.

[0054] Figure 3 for Figure 2 Enlarged structural diagram at point A.

[0055] Figure 4 This is a structural diagram of a first controller according to an embodiment of this application.

[0056] Figure 5 This is a structural diagram of a nucleic acid amplification detection module according to an embodiment of this application.

[0057] Figure 6 for Figure 5 The exploded structure diagram shown.

[0058] Figure 7 This is a structural diagram of a temperature control component according to an embodiment of this application.

[0059] Figure 8 for Figure 7 The exploded view of the temperature control component is shown.

[0060] Figure 9 This is a structural diagram showing the carrier prepared for insertion into a slot according to an embodiment of this application.

[0061] Figure 10 for Figure 9 Enlarged structural diagram at point B.

[0062] Figure 11 This is a structural diagram of a carrier being inserted into a slot according to an embodiment of this application.

[0063] Figure 12 for Figure 11 Enlarged structural diagram at point C.

[0064] Figure 13 This is a structural diagram of a heater according to an embodiment of this application.

[0065] Figure 14 This is a structural diagram of the auxiliary heating element and lead wire of a heater according to an embodiment of this application.

[0066] Figure 15 This is a structural diagram of a fluorescence detection component according to an embodiment of this application.

[0067] Figure 16 for Figure 15 The exploded structure diagram of the fluorescence detection component is shown.

[0068] Figure 17 This is a structural diagram of a power assembly according to an embodiment of this application.

[0069] Figure 18 for Figure 17 The diagram shows a cross-sectional view of the power assembly.

[0070] Figure 19 This is a structural view of a nucleic acid amplification and detection module according to an embodiment of this application.

[0071] Figure 20 for Figure 19 Enlarged structural diagram at point D.

[0072] Figure 21 This is a cross-sectional view of a nucleic acid amplification detection module according to an embodiment of this application.

[0073] Figure 22 for Figure 21 Enlarged structural diagram at point E.

[0074] Figure 23 This is a temperature-time curve of a nucleic acid amplification detection module according to an embodiment of this application operating under two different current conditions.

[0075] Explanation of reference numerals in the attached figures:

[0076] 10. Housing; 11. Mounting section; 20. Nucleic acid amplification and detection module; 21. First controller; 211. First electrical connector; 212. Third electrical connector; 213. Fourth electrical connector; 214. Sixth electrical connector; 22. Temperature control assembly; 221. First circuit board; 2211. First electrical connector; 222. Temperature adjustment mechanism; 2221. Support plate; 22211. First mounting hole; 2222. Heater; 222 21. Support layer; 22222. Main heating element; 22223. Heat spreader; 22224. Auxiliary heating element; 223. Support base; 2231. Slot; 2232. Boss; 22321. First guide surface; 22322. Second guide surface; 23. Fluorescence detection component; 2223. Heat sink; 2224. First lead wire; 2225. Second lead wire; 2226. Thermal conductive element; 2227. Thermal insulation element; 231. Second circuit board; 311. Second electrical connection connector; 232. First support member; 2321. Third mounting hole; 233. Fluorescence detection mechanism; 234. Clamping mechanism; 2341. Guide member; 23411. Second mounting hole; 2342. Movable frame; 23421. Clearance opening; 23422. Movable plate; 23423. Third support member; 2343. Clamping member; 23431. Bearing plate; 23432. Pressure head; 2344. Nut; 2345. 2346. Lead screw; 2347. Coupling; 2348. Linear bearing; 235. Second support member; 24. Power assembly; 241. First power mechanism; 242. Third circuit board; 2421. Fifth electrical connection connector; 243. Second power mechanism; 244. Housing; 2441. Window; 245. Cover plate; 246. Mounting bracket; 2461. With fourth mounting hole; 25. Sunshade; 251. Sunshade plate; 30. Touch screen; 40. Carrier. Detailed Implementation

[0077] To make the above-mentioned objectives, features, and advantages of this application more apparent and understandable, the specific embodiments of this application are described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of this application. However, this application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of this application. Therefore, this application is not limited to the specific embodiments disclosed below.

[0078] Please see Figure 1 and Figure 2 , Figure 1 A schematic diagram of the nucleic acid amplification and detection device according to one embodiment of this application is shown. Figure 2 It shows Figure 1The diagram shows an exploded view of the nucleic acid amplification detection device. One embodiment of this application provides a nucleic acid amplification detection device including a housing 10 and a nucleic acid amplification detection module 20. The housing 10 has a mounting portion 11, which includes, but is not limited to, a mounting slot. In this embodiment, the mounting portion 11 is specifically described as a mounting slot, but this is not a limitation. The nucleic acid amplification detection module 20 is correspondingly and detachably mounted in the mounting slot.

[0079] The number of nucleic acid amplification detection modules 20 is not limited to one; for example, there may be two, three, four, or more. The number of mounting slots is the same as the number of nucleic acid amplification detection modules 20. Each nucleic acid amplification detection module 20 is correspondingly inserted into its respective mounting slot.

[0080] Optionally, each nucleic acid amplification and detection module 20 can operate independently or in coordination. The individual nucleic acid amplification and detection modules 20 are equivalent, allowing for flexible replacement, maintenance, and adjustments to the deployment quantity based on actual needs.

[0081] For example, the nucleic acid amplification detection module 20 includes a first controller 21. The nucleic acid amplification detection device also includes a second controller, which is mounted in the housing 10 and electrically connected to the first controller 21. The second controller is used to control the operation of each first controller 21 and to supply power to each nucleic acid amplification detection module 20. It can also be used for user interaction and data storage, etc.

[0082] The nucleic acid amplification and detection module 20, as the core functional structure of the nucleic acid amplification and detection device, can be assembled from multiple functional components. Specifically, the first controller 21 receives control commands from the second controller, processes them, distributes them to each functional component of the nucleic acid amplification and detection module 20, and controls each functional component to perform its respective function.

[0083] For example, each nucleic acid amplification detection module 20 possesses complete functions such as temperature control and fluorescence detection. The second controller is only used to power each nucleic acid amplification detection module 20 and to enable interaction between the nucleic acid amplification detection module 20 and the user. That is, when the nucleic acid amplification detection module 20 receives, for example, an amplification command and / or a temperature adjustment command, it can adjust and control the temperature of the carrier 40 to complete the nucleic acid amplification operation of the reaction sample within the carrier 40; when the nucleic acid amplification detection module 20 receives, for example, a fluorescence detection command, it can perform fluorescence detection of the reaction sample. In this way, the number of nucleic acid amplification detection modules 20 that the in vitro analytical diagnostic device can carry is not limited, resulting in greater flexibility.

[0084] Specifically, in this embodiment, the first controller 21 has the ability to supply and control current, communicate with other functional components (such as temperature control component 22, fluorescence detection component 23 and power component 24), and has high-precision detection capabilities for current, voltage and resistance. It can supply power to each functional component and is used to control the temperature adjustment of the temperature control component 22, control the fluorescence excitation and reception of the fluorescence detection component 23, and control the operation of the first power mechanism 241 and the second power mechanism 243 of the power component 24.

[0085] Optionally, the mounting slot is adapted to the shape of the nucleic acid amplification detection module 20. When the nucleic acid amplification detection module 20 is inserted into the mounting slot, the mounting slot guides the nucleic acid amplification detection module 20, ensuring that the nucleic acid amplification detection module 20 is stably aligned and installed in the mounting slot. Please also refer to... Figures 2 to 4 The first controller 21 is provided with a first electrical connector 211, which is located, for example, at the bottom of the nucleic acid amplification detection module 20. The second controller is provided with a second electrical connector (not shown in the figure), which is located, for example, at the bottom of the mounting slot. When the nucleic acid amplification detection module 20 is inserted into the mounting slot, the first electrical connector 211 and the second electrical connector are connected to each other to realize the electrical connection between the first controller 21 and the second controller.

[0086] In order to ensure that the nucleic acid amplification detection module 20 is securely installed in the housing 10, optionally, after the nucleic acid amplification detection module 20 is installed in the mounting slot, the nucleic acid amplification detection module 20 and the housing 10 are also fixedly connected by screws, pins, rivets or snap-fit ​​components.

[0087] Optionally, the nucleic acid amplification detection device also includes a touch screen 30. The touch screen 30 is mounted on the housing 10. The touch screen 30 is electrically connected to the second controller. The touch screen 30 can be used to input various information, including but not limited to temperature, time, number of cycles, and sample type.

[0088] Of course, nucleic acid amplification and detection devices may also include various other functional components such as barcode scanners. The specific configuration and adjustment can be flexibly set according to actual needs, and no limitations are imposed here.

[0089] It should be noted that, Figure 1 and Figure 2 The purpose of this illustration is solely to depict the connection relationship between the nucleic acid amplification detection module 20 and the housing 10, and is not to specifically limit the connection positions, specific structures, or quantities of each device. In other embodiments of this application, the nucleic acid amplification detection device may include more or fewer components than illustrated, or combine some components, or split some components, or have different component arrangements. The illustrated components may be implemented in hardware, software, or a combination of software and hardware.

[0090] The nucleic acid amplification and detection module 20, as the core functional unit of the nucleic acid amplification and detection device, is mainly used to complete various operations such as nucleic acid amplification and fluorescence detection of the reaction sample. Its assembly and disassembly efficiency affects the assembly and disassembly efficiency of the nucleic acid amplification and detection device. When the nucleic acid amplification and detection module 20 is divided into multiple functional components according to function, and each functional component is manufactured separately and assembled together, the convenience and efficiency of the nucleic acid amplification and detection device production can be increased.

[0091] Please refer to the following: Figure 5 and Figure 6 Specifically, the nucleic acid amplification detection module 20 also includes a temperature control component 22 and a fluorescence detection component 23. The temperature control component 22 is used to raise or lower the temperature of the carrier 40, so that the reaction sample within the carrier 40 undergoes repeated heating and cooling cycles to achieve amplification. The fluorescence detection component 23 is used to detect the carrier 40 and perform fluorescence detection on the nucleic acid amplification products within the carrier 40.

[0092] To facilitate electrical connection between the first controller 21 and the temperature control component 22 and the fluorescence detection component 23, the temperature control component 22 may optionally have a first electrical connection connector 2211. The fluorescence detection component 23 may have a second electrical connection connector 2311. The first controller 21 may have a third electrical connection connector 212 and a fourth electrical connection connector 213. The first electrical connection connector 2211 and the third electrical connection connector 212 are plugged into each other to achieve electrical connection between the temperature control component 22 and the controller. The first controller 21 can supply power to the temperature control component 22 and can also transmit information with the temperature control component 22, acquiring temperature information from the temperature control component 22 and sending various control commands, including heating and / or cooling, to the temperature control component 22, thereby achieving precise adjustment and control of the temperature of the carrier 40. The second electrical connection connector 2311 and the fourth electrical connection connector 213 are plugged into each other to achieve electrical connection between the fluorescence detection component 23 and the controller. The first controller 21 can supply power to the fluorescence detection component 23, and can also transmit information with the fluorescence detection component 23. It can send various instructions such as fluorescence detection to the fluorescence detection component 23, and can acquire the fluorescence detection signal of the fluorescence detection component 23.

[0093] During assembly, the temperature control component 22 and the first controller 21 can be electrically connected by plugging in the first electrical connector 2211 and the third electrical connector 212, and the fluorescence detection component 23 and the first controller 21 can be electrically connected by plugging in the second electrical connector 2311 and the fourth electrical connector 213. The assembly efficiency is high and the labor cost is reduced.

[0094] The electrical connection between the first electrical connector 2211 and the third electrical connector 212 can be achieved by an easy-to-plug electrical connector, making the electrical connection simple and reliable.

[0095] For example, the temperature control component 22 and the fluorescence detection component 23 are both arranged on either side of the first controller 21, specifically on the top side of the first controller 21.

[0096] Of course, the temperature control component 22, the fluorescence detection component 23, and the first controller 21 can also be flexibly arranged and adjusted in various other ways, without limitation, as long as they meet the following requirements: on the one hand, the temperature control component 22 is in contact with one side of the carrier 40 to raise or lower the temperature of the carrier 40 and complete the nucleic acid amplification operation; on the other hand, the fluorescence detection component 23 is set opposite to the other side of the carrier 40 so as to realize the fluorescence detection operation of the reaction sample in the carrier 40.

[0097] Since the temperature control component 22 and the fluorescence detection component 23 are each connected to the electrical connection connector of the first controller 21 via electrical connectors, the temperature control component 22, the fluorescence detection component 23, and the first controller 21 are assembled together. To further improve the stability of the assembly of the temperature control component 22, the fluorescence detection component 23, and the first controller 21, they can be further connected and secured to each other using connectors. For example, the temperature control component 22 and the fluorescence detection component 23 are connected by fasteners such as screws, pins, rivets, or bolts. This strengthens the connection between the temperature control component 22 and the fluorescence detection component 23, making it less prone to loosening.

[0098] Please see Figure 5 and Figure 6 For example, the temperature control component 22 includes a first circuit board 221, and a first electrical connector 2211 is connected to the first circuit board 221. The fluorescence detection component 23 includes a second circuit board 231, and a second electrical connector 2311 is connected to the second circuit board 231. This simplifies the wiring and reduces the overall structure.

[0099] Please see Figure 6 , Figure 9 and Figure 10 For example, the carrier 40 is disposed between the temperature control component 22 and the fluorescence detection component 23. The side of the temperature control component 22 facing the fluorescence detection component 23 abuts against the carrier 40, enabling it to raise or lower the temperature of the carrier 40, thereby raising or lowering the temperature of the reaction sample within the carrier 40 to complete the nucleic acid amplification of the reaction sample. The other side of the carrier 40 is disposed opposite to the fluorescence detection component 23, which is used to emit excitation light to irradiate the reaction sample and to receive the light signal returned from the reaction sample and convert it into an electrical signal, thereby achieving fluorescence detection.

[0100] For example, a flat-structured carrier 40 is inserted between the fluorescence detection component 23 and the temperature control component 22. It is understood that a flat structure can refer to a carrier 40 whose thickness direction dimension is much smaller than its dimension perpendicular to the thickness direction. For example, the ratio of the dimension perpendicular to the thickness direction to the thickness direction dimension is greater than 5:1, such as a ratio of 50 to 100:1, or, for example, 90:1. For example, the carrier 40 is approximately a cuboid, and the ratio of its length to its thickness can be greater than 5:1, such as 90:1. For example, the thickness direction dimension of the carrier 40 can be 0.3 mm to 1.0 mm, the width of the carrier 40 can be 8 mm to 12 mm, and the length of the carrier 40 can be 18 mm to 22 mm. Of course, the cross-section of the carrier 40 can be cylindrical, polygonal, or elliptical, etc.

[0101] The flat structure of the carrier 40 results in a very thin reaction sample within it, with a small distance between the center of the reaction sample and the liquid surface. When the carrier 40 is heated or cooled by the temperature control component 22, the temperature of the reaction sample can reach uniformity in a very short time, resulting in high heat transfer efficiency and significantly improved heating / cooling rates and detection efficiency. In contrast, the inner diameter of the PCR tube is larger than that of the flat carrier 40, leading to a greater distance between the center of the reaction sample and the liquid surface. This results in a longer time for the temperature of the reaction sample to reach uniformity, slower heating / cooling rates, and lower detection efficiency.

[0102] Furthermore, the carrier 40 is inserted between the fluorescence detection component 23 and the temperature control component 22. Neither the fluorescence detection component 23 nor the temperature control component 22 needs to move to allow the carrier 40 to be placed or removed. This reduces the need for extra space for the fluorescence detection component 23 and the temperature control component 22, improving the compactness of the nucleic acid amplification fluorescence detection component 23. Simultaneously, the wiring connected to the fluorescence detection component 23 and the wiring harnesses and tubing connected to the temperature control component 22 do not require excess length, saving space. Additionally, since neither the fluorescence detection component 23 nor the temperature control component 22 needs to move, the nucleic acid amplification detection module 20 does not need to be configured with structures that allow for movement of the fluorescence detection component 23 or the temperature control component 22, simplifying the structure of the nucleic acid amplification detection module 20 and facilitating its miniaturization.

[0103] Please see Figure 7 , Figure 8 , Figure 10 and Figure 12Based on any of the foregoing embodiments, the temperature control component 22 includes a temperature adjustment mechanism 222 and a support base 223. The temperature adjustment mechanism 222 is spaced apart from the fluorescence detection component 23. The support base 223 is located on the side of the temperature adjustment mechanism 222 facing the fluorescence detection component 23, and the support base 223 has a slot 2231 for inserting the carrier 40. The slot 2231 passes through the support base 223 along the arrangement direction X of the fluorescence detection component 23 and the temperature adjustment mechanism 222. Thus, after the carrier 40 is inserted into the slot 2231, one side of the carrier 40 along the arrangement direction can abut against the temperature adjustment mechanism 222, and the fluorescence detection component 23 detects the reaction sample through the other side of the carrier 40 along the arrangement direction.

[0104] Optionally, the temperature control assembly 22 further includes a first mounting component, which includes, but is not limited to, screws. The support base 223 is mounted to the temperature regulating mechanism 222 via the first mounting component to achieve fixation to the temperature regulating mechanism 222. The carrier 40 is inserted between the temperature regulating mechanism 222 and the fluorescence detection assembly 23 by being inserted into the slot 2231. Optionally, the slot 2231 can provide approximate positioning and guidance for the insertion of the carrier 40. The upper end of the slot 2231 has an inlet end, and the carrier 40 is inserted into the slot 2231 vertically from top to bottom from the inlet end.

[0105] Please see Figure 15 and Figure 16 For example, the fluorescence detection component 23 includes a first support member 232, a fluorescence detection mechanism 233, and a pressing mechanism 234. Optionally, the first support member 232 may include, but is not limited to, a support plate, a support block, or a support frame. In this embodiment, a support plate is preferred because it occupies less space.

[0106] The number of fluorescence detection units 233 can be one or more, such as two, three, four, or other numbers. When there are multiple fluorescence detection units 233, each of them includes a light source. The light sources of the multiple fluorescence detection units 233 are light sources of different wavelengths. Thus, the wavelength of fluorescence generated by the fluorescence detection units 233 is determined according to the wavelength of the light source. Multiple light sources of different wavelengths correspond to multiple different wavelengths of fluorescence, that is, different wavelengths of fluorescence can be provided to perform fluorescence detection on the reaction sample of the carrier 40, ensuring fluorescence detection effect and efficiency, while meeting the needs of different detection items. In this embodiment, there are specifically five fluorescence detection units 233, which correspondingly generate five different wavelengths of fluorescence. The specific wavelengths of the five fluorescences are set according to actual needs and are not limited here.

[0107] As an alternative, among the multiple fluorescence detection units 233, two, three, or other numbers of fluorescence detection units 233 may provide light sources in the same wavelength band. Of course, the light sources of the multiple fluorescence detection units 233 may also be completely different wavelength bands.

[0108] It is understandable that the fluorescence detection mechanism 233 may also include components such as a fluorescence detector, a filter, a convex lens, and a dichroic mirror, which will not be described in detail here.

[0109] Please see Figure 6 , Figure 7 , Figure 15 and Figure 16 For example, the fluorescence detection mechanism 233 is disposed on the first support member 232, which serves to support and bear the fluorescence detection mechanism 233. The fluorescence detection mechanism 233 is used to detect the carrier 40, so as to perform fluorescence detection on the product of nucleic acid amplification of the reaction sample within the carrier 40. The clamping mechanism 234 is disposed on the first support member 232, which also serves to support and bear the clamping mechanism 234. The clamping mechanism 234 is used to press the carrier 40 against the temperature regulating mechanism 222 or to release the carrier 40. Both the clamping mechanism 234 and the fluorescence detection mechanism 233 are installed on the first support member 232, resulting in a compact overall structure and a small footprint. After the carrier 40 is inserted into the slot 2231, the clamping mechanism 234 presses the carrier 40 against the temperature regulating mechanism 222, resulting in good heat transfer. The temperature regulating mechanism 222 can more precisely control and adjust the temperature of the reaction sample inside the carrier 40. Furthermore, by reducing the gap between the carrier 40 and the temperature regulating mechanism 222, the distance between the carrier 40 and the fluorescence detection mechanism 233 remains constant, thereby ensuring the accuracy and reliability of the detection results of the fluorescence detection mechanism 233. After the fluorescence detection of the reaction sample inside the carrier 40 is completed, the clamping mechanism 234 releases the carrier 40.

[0110] Please see Figure 6 , Figure 17 and Figure 18 For example, the nucleic acid amplification detection module 20 also includes a power assembly 24. The power assembly 24 is primarily responsible for providing power to the various moving parts of the nucleic acid amplification detection module 20, and includes a first power mechanism 241. The first power mechanism 241 is connected to the clamping mechanism 234 and provides power to move the clamping mechanism 234 to clamp or release the carrier 40. The clamping mechanism 234 avoids the fluorescence detection mechanism 233 along its direction of movement. This ensures that the clamping mechanism 234 and the fluorescence detection mechanism 233 do not interfere with each other during movement, allowing the clamping or releasing of the carrier 40 to be carried out smoothly. Furthermore, the fluorescence detection of the amplified products within the carrier 40 by the fluorescence detection mechanism 233 is not interfered with by the clamping mechanism 234.

[0111] Optionally, the power assembly 24 further includes a fifth electrical connector 2421, to which the first power mechanism 241 is electrically connected. The first controller 21 has a sixth electrical connector 214, with the fifth electrical connector 2421 and the sixth electrical connector 214 engaging. Thus, similar to the temperature control assembly 22 and the fluorescence detection assembly 23, the electrical connection between the power assembly 24 and the first controller 21 is achieved through the engagement of the fifth and sixth electrical connection structures, resulting in higher assembly efficiency and reduced labor costs. Furthermore, the first controller 21 can supply power to the power assembly 24 and transmit control commands to it, allowing the power assembly 24 to operate according to the control commands from the first controller 21.

[0112] Optionally, the first power mechanism 241 may include, but is not limited to, a motor, a cylinder, a hydraulic cylinder, etc., as long as it can provide power to the clamping mechanism 234 so that the clamping mechanism 234 can clamp or release the carrier 40. In this embodiment, the first power mechanism 241 is specifically described using a motor as an example, but it is not limited thereto.

[0113] In one specific embodiment, the first power mechanism 241 includes a motor. The motor is, for example, a stepper motor.

[0114] Please see Figure 15 and Figure 16 The clamping mechanism 234 includes a guide member 2341, which is connected to the first support member 232. Optionally, the guide member 2341 may be, but is not limited to, a guide post or a guide rod, as long as it can guide the movement of the movable frame 2342 and facilitate the smooth movement of the movable frame 2342.

[0115] The clamping mechanism 234 also includes a movable frame 2342. The movable frame 2342 is slidably disposed on the guide member 2341.

[0116] The clamping mechanism 234 also includes a clamping member 2343. The clamping member 2343 is connected to the movable frame 2342, which can drive the clamping member 2343 to move, causing the clamping member 2343 to clamp or release the carrier 40. The direction of movement of the clamping member 2343 is as follows: Figure 15 As shown by the double arrow X in the diagram. In this embodiment, the movement direction of the clamping member 2343 is also set along the longitudinal direction of the housing 244, as shown in the diagram. Figure 1 The double arrow X is shown in the diagram.

[0117] The clamping mechanism 234 also includes a nut 2344 and a lead screw 2345. The nut 2344 is connected to the movable frame 2342. The lead screw 2345 passes through the nut 2344 and is connected to the motor shaft.

[0118] When the motor rotates, it drives the lead screw 2345 to rotate, causing the nut 2344 to reciprocate along the axial direction of the lead screw 2345. The nut 2344 synchronously drives the moving frame 2342 to move, and the moving frame 2342 correspondingly drives the clamping member 2343 to clamp the carrier 40 or loosen the carrier 40.

[0119] Please see Figures 15 to 18 For example, the clamping mechanism 234 also includes a coupling 2346. The coupling 2346 is mounted on the first support member 232, and the lead screw 2345 is coaxially connected to the coupling 2346, which is connected to the motor shaft. Thus, the coupling 2346 facilitates the connection between the lead screw 2345 and the motor shaft, improving assembly efficiency. The coupling 2346 receives power from the motor; when it rotates, it drives the lead screw 2345 to rotate synchronously, thereby causing the nut 2344 to reciprocate along the axial direction of the lead screw 2345, thus moving the movable frame 2342 and clamping or loosening the carrier 40.

[0120] To facilitate smooth sliding of the movable frame 2342 on the guide member 2341, the clamping mechanism 234 optionally includes a linear bearing 2347 connected to the movable frame 2342. The guide member 2341 passes through the linear bearing 2347. The number of guide members 2341 is not limited to one; for example, there may be multiple guide members 2341, and the number of linear bearings 2347 is the same as the number of guide members 2341. Each guide member 2341 passes through its respective linear bearing 2347.

[0121] Please see Figure 19 and Figure 20 For example, one end of the guide member 2341 is connected to the first support member 232, and the other end of the guide member 2341 is detachably connected to the temperature control component 22. Specifically, the guide member 2341 is connected to the support plate 2221. In this way, the guide member 2341 is detachably connected to the temperature control component 22, so that the temperature control component 22 and the fluorescence detection component 23 are interconnected, which can ensure the stability of the connection between the temperature control component 22 and the fluorescence detection component 23 and prevent displacement due to external environmental vibration.

[0122] Specifically, the clamping mechanism 234 also includes a second mounting component. The second mounting component includes, but is not limited to, screws, pins, or rivets. The other end of the guide member 2341 is connected and fixed to the temperature regulating mechanism 222 and / or the support base 223 via the second mounting component.

[0123] Optionally, please refer to Figure 19 and Figure 20The temperature regulating mechanism 222 is provided with a first mounting hole 22211, and the guide member 2341 is provided with a second mounting hole 23411. The second mounting member passes through the first mounting hole 22211 and the second mounting hole 23411 to connect and fix the guide member 2341 to the temperature regulating mechanism 222.

[0124] Please see Figure 15 For example, the clamping component 2343 includes a support plate 23431 and a pressure head 23432. The support plate 23431 is connected to the movable frame 2342 and reciprocates with the movable frame 2342. The pressure head 23432 is connected to the support plate 23431, and the support plate 23431 is used to support the pressure head 23432, providing structural support. Optionally, the support plate 23431 may be made of a rigid material, which is not easily deformed under stress and has a long service life. Specific examples include, but are not limited to, metal plates and / or rigid non-metallic plates, which can be flexibly selected according to actual needs. Optionally, the pressure head 23432 may be made of a heat-insulating material, specifically, non-metallic materials such as plastic. The pressure head 23432 is used to clamp or loosen the carrier 40. When the pressure head 23432 comes into contact with the carrier 40, it can effectively reduce the heat transfer from the carrier 40 to the pressure head 23432, so that the temperature of the carrier 40 can be precisely controlled and is not affected by external components.

[0125] Optionally, the pressure head 23432 may be annular, among other things, and is pressed against the outer periphery of the carrier 40, thereby avoiding the chamber of the carrier 40 and preventing obstruction of the detection light of the fluorescence detection component 23, ensuring that the fluorescence detection component 23 can normally detect the reaction sample inside the carrier 40. Furthermore, the support plate 23431 has an opening that prevents obstruction of the detection light of the fluorescence detection component 23, and the pressure head 23432 is arranged circumferentially around the opening. Additionally, when the carrier 40 is pressed by the clamping member 2343, the carrier 40 is subjected to uniform force around its periphery, allowing it to be smoothly pressed against the temperature regulating mechanism 222.

[0126] Please see Figure 15 and Figure 16For example, the fluorescence detection assembly 23 further includes a second support member 235. The second support member 235 is connected between the fluorescence detection mechanism 233 and the first support member 232. That is, the fluorescence detection mechanism 233 and the first support member 232 are indirectly connected. The first support member 232 supports the second support member 235, and the second support member 235 is installed and supports the fluorescence detection mechanism 233, so that there is a gap between the fluorescence detection mechanism 233 and the first support member 232. Optionally, the second support member 235 may include, but is not limited to, a support plate, a support block, or a support frame. In this embodiment, the second support member 235 is specifically a support frame, such as a U-shaped frame or a cover, which facilitates the formation of a receiving space between the fluorescence detection mechanism 233 and the first support member 232. This receiving space can accommodate the lead screw 2345 and the coupling 2346, thereby avoiding interference between the fluorescence detection mechanism 233 and the lead screw 2345 and the coupling 2346. Optionally, the movable frame 2342 is provided with a clearance opening 23421 to avoid the second support member 235, and the second support member 235 passes through the clearance opening 23421. In this way, when the movable frame 2342 is moved by the first power mechanism 241, it can avoid the second support member 235 and avoid interference with the second support member 235.

[0127] It should be noted that the number of fluorescence detection mechanisms 233 is not limited, for example, one or more. In this embodiment, in order to improve the fluorescence detection speed of the reaction sample in the carrier 40, multiple fluorescence detection mechanisms 233 are specifically configured. Optionally, the multiple fluorescence detection mechanisms 233 are arranged sequentially. Specifically, the multiple fluorescence detection mechanisms 233 can be stacked together sequentially in the vertical direction, that is, the arrangement direction of the multiple fluorescence detection mechanisms 233 is preferably consistent with the length direction of the carrier 40, so as to realize fluorescence detection of multiple different parts of the carrier 40 from top to bottom. Of course, the multiple fluorescence detection mechanisms 233 can also be arranged sequentially in the horizontal direction or other directions, or can be arranged in various ways such as regularity and irregularity, all of which are within the protection scope of this application. The specific arrangement method is not limited here, as long as the detection end of each fluorescence detection mechanism 233 is arranged opposite to the reaction chamber of the carrier 40, so as to be able to perform fluorescence detection of nucleic acid amplification products in the carrier 40.

[0128] For example, the movable frame 2342 includes a movable plate 23422 and a third support member 23423. A nut 2344 is connected to the movable plate 23422, the movable plate 23422 is slidably disposed on the guide member 2341, the movable plate 23422 and the first support member 232 are disposed at a distance from each other, and the fluorescence detection mechanism 233 is located on the side of the movable plate 23422 opposite to the first support member 232; the third support member 23423 is connected between the clamping member 2343 and the movable plate 23422.

[0129] Optionally, the third support member 23423 may include, but is not limited to, a support column or support rod. The number of third support members 23423 may include, but is not limited to, one or more. To stably support the clamping member 2343, multiple third support members 23423 may be provided. Furthermore, multiple third support members 23423 are arranged at intervals around the outer periphery of the second support member 235 and the fluorescence detection mechanism 233, thereby avoiding interference with the second support member 235 and the fluorescence detection mechanism 233 during movement, while maintaining a compact overall structure and occupying less space.

[0130] Please see Figure 17 and Figure 18 For example, the power assembly 24 also includes a second power mechanism 243. The second power mechanism 243 is electrically connected to the fifth electrical connection connector 2421. The power assembly 24 also includes a housing 244, which has a window 2441 and a cover plate 245 slidably disposed at the window 2441. The window 2441 and the slot 2231 are positioned opposite each other. The second power mechanism 243 is connected to the cover plate 245 and is used to drive the cover plate 245 to slide, thereby opening or closing the window 2441. Thus, when the second power mechanism 243 drives the cover plate 245 to open the window 2441, the carrier 40 can be placed into the slot 2231 of the support 223 through the window 2441, and the carrier 40 in the support 223 can be taken out through the window 2441. Furthermore, the opening and closing of the cover plate 245 and the pressing of the carrier 40 are driven by their respective power mechanisms, rather than sharing a single power mechanism. This allows the opening and closing of the cover plate 245 and the pressing of the carrier 40 to be achieved independently, avoiding mutual interference caused by sharing a single power mechanism and thus providing greater flexibility.

[0131] For example, the power assembly 24 also includes a third circuit board 242. A fifth electrical connector 2421 is connected to the third circuit board 242.

[0132] Optionally, the second power mechanism 243 may include, but is not limited to, a linear mechanism. There are many specific forms of linear mechanisms. For example, it may be similar to the form in which the motor, lead screw 2345 and nut 2344 cooperate with each other in the above embodiment. It may also be a cylinder or hydraulic cylinder, or a cam mechanism, gear mechanism or crank-slider mechanism, etc. There are no limitations here, as long as it can push the cover plate 245 to move.

[0133] In one embodiment, the second power mechanism 243 includes, for example, a push rod motor and a slider that can move linearly between two points, a distal end and a proximal end. The slider is slidably disposed on the top wall of the housing 244. The cover plate 245 is fixedly connected to the slider. The push rod motor is connected to the slider and is used to push the slider to slide, thereby realizing the opening and closing action of the cover plate 245.

[0134] In this embodiment, the opening and closing of the cover plate 245 and the loading and unloading of the carrier 40 are each driven by a power mechanism, which is highly flexible and can be easily automated.

[0135] For example, the power assembly 24 also includes a mounting bracket 246. The mounting bracket 246 is connected to the housing 244, and the first power mechanism 241 and the second power mechanism 243 are mounted on the mounting bracket 246.

[0136] Optionally, the mounting bracket 246 is detachably connected to the first support member 232. In this way, the power assembly 24 and the fluorescence detection assembly 23 are interconnected, which can ensure the stability of the connection between the power assembly 24 and the fluorescence detection assembly 23 and prevent displacement due to vibration of the external environment.

[0137] Specifically, the power assembly 24 also includes a third mounting component. The third mounting component includes, but is not limited to, screws, pins, or rivets. The mounting bracket 246 is connected and secured to the first support member 232 via the third mounting component.

[0138] Optionally, please refer to Figure 21 and Figure 22 The first support member 232 is provided with a third mounting hole 2321, and the mounting bracket 246 is provided with a fourth mounting hole 2461. The third mounting member passes through the third mounting hole 2321 and the fourth mounting hole to connect and fix the first support member 232 and the mounting bracket 246.

[0139] To improve the stability of the connection between the temperature control component 22, the fluorescence detection component 23, the power component 24, and the first controller 21, after the electrical connection connectors between the temperature control component 22 and the first controller 21 are plugged in and assembled, the first controller 21 and the temperature control component 22 can also be connected and fixed using fasteners such as screws, pins, or rivets. Similarly, after the electrical connection connectors between the fluorescence detection component 23 and the first controller 21 are plugged in and assembled, the first controller 21 and the fluorescence detection component 23 can also be connected and fixed using fasteners such as screws, pins, or rivets.

[0140] For example, the housing 244 covers the top of the temperature control component 22 and the fluorescence detection component 23. Thus, when the nucleic acid amplification detection module 20 is inserted into the mounting slot and installed in the housing 10, the temperature control component 22 and the fluorescence detection component 23 are protected by the housing 10 within the mounting slot, eliminating the need for the housing 244. Since the housing 244 is located on top of the temperature control component 22 and the fluorescence detection component 23, it seals the opening of the mounting slot, preventing the tops of the temperature control component 22 and the fluorescence detection component 23 from being exposed, thus protecting them.

[0141] When multiple nucleic acid amplification detection modules 20 are installed inside the housing 10, if the side of the fluorescence detection mechanism 233 is not covered by the housing 244, they will interfere with each other and affect the fluorescence detection results during the fluorescence detection process.

[0142] For example, the nucleic acid amplification detection module 20 also includes a light shield 25, which is connected to the housing 244, the first controller 21, or the mounting bracket 246, and is positioned over the fluorescence detection mechanism 233. Thus, the light shield 25 serves to block light, preventing the various nucleic acid amplification detection modules 20 from interfering with each other during fluorescence detection.

[0143] Among them, each nucleic acid amplification and detection module 20 is arranged sequentially along the Y direction inside the casing 10, for example, as shown in the figure. Figure 1 As shown in the figure. Optionally, the light shield 25 includes two light shields 251, which are located on opposite sides of the fluorescence detection mechanism 233 along the Y direction.

[0144] Please see Figure 8 , Figure 13 and Figure 14 For example, the temperature regulating mechanism 222 includes a heater 2222, which is used to contact the carrier 40 to raise the temperature of the carrier 40. The heater 2222 can be of various types, including but not limited to heating methods using resistance wires or using semiconductor coolers.

[0145] Specifically, the heater 2222 includes a support layer 22221, a main heating element 22222, and a heat spreader 22223. The main heating element 22222 includes, but is not limited to, a main heating coil, which is connected to the support layer 22221. The support layer 22221 serves to support and carry the main heating coil. Optionally, the main heating coil may be formed on the support layer 22221 using, but is not limited to, circuit board manufacturing or 3D printing processes.

[0146] For example, the temperature distribution plate 22223 is connected to the support layer 22221. When the main heating element 22222 is energized, it transfers temperature to the temperature distribution plate 22223. The temperature of different parts of the surface of the temperature distribution plate 22223 is relatively uniform, which can improve the temperature uniformity of the reaction sample in different parts of the carrier 40 when it comes into contact with the carrier 40.

[0147] For example, the temperature regulating mechanism 222 also includes a heat sink 2223. The heat sink 2223 is connected to the heater 2222 and is used to reduce the temperature of the heater 2222. Specifically, the heat sink 2223 is connected to the side of the support layer 22221 opposite to the heat spreader 22223. When the heat sink 2223 is working, the heat from the heater 2222 can be transferred to the heat sink 2223 through the support layer 22221, and the heat sink 2223 carries away the heat, thereby reducing the temperature of the heater 2222 and thus controlling the temperature of the carrier 40. In addition, when the operating power of the heater 2222 decreases, its own operating temperature also decreases, thereby reducing the temperature of the carrier 40; conversely, when the operating power of the heater 2222 increases, its temperature increases, thereby increasing the temperature of the carrier 40.

[0148] However, achieving temperature uniformity of 0.2℃ (the difference between the maximum and minimum temperatures) over a large area solely through the temperature equalization plate 22223 presents significant challenges, thus affecting the accuracy of fluorescence detection results. Therefore, in this embodiment, the heater 2222 further includes an auxiliary heating element 22224, which includes, but is not limited to, an auxiliary heating coil connected to the temperature equalization plate 22223. Applying different power to the auxiliary heating coil can achieve temperature uniformity over a large area. The number of auxiliary heating coils can be one or more, for example. Multiple coils can be two, three, four, or more, and can be flexibly adjusted and set according to actual needs, such as the size of the heating area / temperature uniformity requirements, and are not limited here.

[0149] During the temperature control process, the main heating element 22222 plays the main role in power regulation, realizing a wide range of temperature adjustment. After the auxiliary heating element 22224, which has been calibrated by an external sensor, senses the temperature difference, each auxiliary heating element 22224 applies different power (or the same power) to achieve a small range of temperature compensation, thereby achieving temperature uniformity.

[0150] The heating power of the main heating element 22222 mainly depends on the thermal resistance between the main heating element 22222 and the heat sink 2223. Therefore, the support layer 22221 is preferably made of a material with low thermal conductivity, such as epoxy resin and glass fiber composite board (FR4), ceramic, polyethylene terephthalate (PET), and / or glass. The thermal resistance of the support layer 22221 needs to balance the relationship between power and heating / cooling rate; the higher the thermal resistance, the lower the heating power and the slower the heating / cooling rate.

[0151] The shape of the temperature distribution plate 22223 is adapted to the shape of the reaction chamber of the carrier 40, which enables heat to be uniformly transferred to different parts of the reaction chamber, which helps to ensure temperature uniformity and thus improves the accuracy and reliability of fluorescence detection results.

[0152] For example, the outer periphery shape of the heat spreader 22223 may include, but is not limited to, a rectangle, a circle, or other irregular shapes. In this embodiment, a rectangular outer periphery shape of the heat spreader 22223 is used as an example, but it is not limited to this.

[0153] The arrangement of the multiple auxiliary heating elements 22224 on the surface of the heat spreader 22223 is not limited here. It can be adjusted and set according to actual needs, as long as it can heat different parts of the surface of the heat spreader 22223, thereby improving the temperature uniformity of different parts of the surface of the heat spreader 22223. Optionally, the multiple auxiliary heating elements 22224 are respectively arranged in multiple different parts of the heat spreader 22223. In other words, the auxiliary heating elements 22224 are arranged in different positions on the heat spreader 22223, thereby achieving temperature compensation and heating of different parts of the surface of the heat spreader 22223, thereby improving the temperature uniformity of the surface of the heat spreader 22223.

[0154] The outer periphery of the heat spreader 22223 is rectangular. For example, the number of auxiliary heating elements 22224 is three, arranged sequentially along the length of the heat spreader 22223. The three auxiliary heating elements 22224 are located in the middle of the heat spreader 22223. The heat spreader 22223 is made of, but is not limited to, ceramic material. Leads are also provided on the surface of the heat spreader 22223. The leads are electrically connected to the auxiliary heating elements 22224. The leads are also electrically connected to the first circuit board 221. The auxiliary heating elements 22224 can be externally connected to the first circuit board 221 via the leads, thereby enabling power supply to the auxiliary heating elements 22224 and control of the current magnitude of the auxiliary heating elements 22224.

[0155] Optionally, each auxiliary heating element 22224 has four leads. Two leads are first leads 2224, which are connected to a voltage testing circuit to measure the operating voltage of the auxiliary heating element 22224. The other two leads are second leads 2225, which are connected to a current supply circuit to supply current to the auxiliary heating element 22224, allowing it to heat up. Furthermore, the resistance value of the auxiliary heating element 22224 can be calculated based on its operating voltage and the current supplied. This resistance value can then be used to determine whether the temperature uniformity of different parts of the surface of the heat spreader 22223 meets the requirements. When the temperature uniformity of different parts of the surface of the heat spreader 22223 is low, the temperature uniformity can be increased by adjusting the current of one or more auxiliary heating elements 22224. For example, if the temperature of a certain part of the surface of the heat spreader 22223 is too high, the auxiliary heating element 22224 corresponding to the high-temperature part of the surface of the heat spreader 22223 will reduce its current value, thereby lowering the temperature of the high-temperature part of the surface of the heat spreader 22223; conversely, if the temperature of a certain part of the surface of the heat spreader 22223 is too low, the auxiliary heating element 22224 corresponding to the low-temperature part of the surface of the heat spreader 22223 will increase its current value, thereby increasing the temperature of the low-temperature part of the surface of the heat spreader 22223. Thus, the heat spreader 22223 can provide a surface with uniform temperature that can be precisely and rapidly controlled.

[0156] For example, the temperature regulating mechanism 222 also includes a heat-conducting element 2226. The heat-conducting element 2226 is made of a metallic material, such as copper, which has good thermal conductivity. The heat-conducting element 2226 connects the heater 2222 and the heat sink 2223. Thus, the heat-conducting element 2226 has good contact performance with the heater 2222 and the heat sink 2223, effectively conducting heat and supporting the heater 2222, improving the installation stability of the heater 2222, and preventing cracking defects caused by insufficient thickness of the heater 2222.

[0157] When cooling is required, the heat from the heater 2222 is quickly directed to the radiator 2223 through the heat conductor 2226 and dissipated by the radiator 2223. At the same time, due to its large heat capacity and the thermal resistance design of the heater 2222, it can be kept at a low temperature.

[0158] It should be noted that the heat sink 2223 in this embodiment includes, but is not limited to, various types such as air-cooled or water-cooled finned heat sink 2223, or semiconductor chip heat sink 2223, etc., and is not limited here. It can be selected according to actual needs. In addition, the cold end of the heat sink 2223 can either directly contact the heat conductor 2226 for heat dissipation, or an intermediate heat transfer medium can be provided between the cold end of the heat sink 2223 and the heat conductor 2226 to achieve indirect heat dissipation. The intermediate heat transfer medium is, for example, a heat exchange medium pipe, which can be a water-cooled copper pipe. The heat from the heater 2222 is transferred to the heat conductor 2226, then from the heat conductor 2226 to the intermediate heat transfer medium, and finally from the intermediate heat transfer medium to the heat sink 2223. As long as it has a good heat dissipation effect and can quickly remove the heat from the heater 2222 to adjust the temperature of the carrier 40 in conjunction with the heater 2222, the specific connection method between the heat sink 2223 and the heat conductor 2226 is not limited in this embodiment.

[0159] To reduce space usage while ensuring effective heat dissipation, the heat sink 2223 in this embodiment is specifically, for example, a tower-type heat pipe air-cooled heat sink 2223. Figure 8 or Figure 9 As shown, the cold end of the tower-type heat pipe air-cooled radiator 2223 is directly connected to the heat-conducting component 2226 via thermally conductive silicone grease. This results in a compact overall structure, with a small temperature regulation mechanism 222 occupying minimal space. Furthermore, the heat from the heater 2222 can be quickly transferred to the radiator 2223 via the heat-conducting component 2226 and dissipated by the radiator 2223, achieving good heat dissipation efficiency.

[0160] For example, the temperature regulating mechanism 222 also includes a first circuit board 221. The first circuit board 221 is electrically connected to the heater 2222. A first electrical connection connector 2211 is disposed on the first circuit board 221. Specifically, both the main heating element 22222 and the auxiliary heating element 22224 are electrically connected to the first circuit board 221. The first circuit board 221 is used to supply power to the main heating element 22222 and the auxiliary heating element 22224, and can control the main heating element 22222 and the auxiliary heating element 22224 to adjust their operating power.

[0161] Optionally, the heat sink 2223 includes a cooling fan to provide heat dissipation for cooling the surface of the vapor chamber 22223. The heat sink 2223 is electrically connected to the first circuit board 221. The first circuit board 221 can supply power to the cooling fan and can also control various operations such as starting the cooling fan, adjusting the airflow, and turning it off.

[0162] For example, the temperature regulating mechanism 222 also includes a support plate 2221. The first circuit board 221 is connected to the support plate 2221, which is used to support and carry the first circuit board 221. The support plate 2221 may be, but is not limited to, a metal plate or a rigid non-metallic plate, to ensure structural strength.

[0163] Optionally, the first circuit board 221 has a first clearance portion, and the support plate 2221 has a second clearance portion. The first clearance portion includes, but is not limited to, clearance holes or notches. The first clearance portion and the second clearance portion are aligned and connected along the thickness direction of the support plate 2221. The heat-conducting component 2226 passes through the second clearance portion and the first clearance portion in sequence and is connected to the heat sink 2223. Specifically, the heat-conducting component 2226 includes, but is not limited to, a heat-conducting block. Furthermore, the heat-conducting component 2226 has a protrusion, which passes through the second clearance portion and the first clearance portion in sequence and is connected to the heat sink 2223.

[0164] For example, a heat insulation member 2227 is provided on the support plate 2221, and the heat insulation member 2227 is located on the side of the support plate 2221 facing the heater 2222. The heat insulation member 2227 is arranged circumferentially around the relief portion, for example. The heat insulation member 2227 includes, but is not limited to, a heat insulation ring. The heat insulation member 2227 is disposed between the support plate 2221 and the heat conducting member 2226, which can prevent the heat conducting member 2226 from contacting the support plate 2221 and causing the temperature of the support plate 2221 to rise, and prevent heat from being conducted to the first circuit board 221. As a result, the temperature control of the carrier 40 is less affected by the first circuit board 221, and the temperature of the carrier 40 can be controlled more accurately.

[0165] The heat-conducting component 2226 includes, but is not limited to, a heat-conducting block. The heat-conducting block has a protrusion that passes through a clearance portion and connects to the heat sink 2223, for example, via thermally conductive adhesive. When the heat sink 2223 is operating, it reduces the temperature of the heat-conducting component 2226. Specifically, the periphery of the heat-conducting component 2226 abuts against the support plate 2221 via a heat insulation component 2227. Furthermore, the temperature control assembly 22 also includes a fourth mounting component. The heat-conducting block is connected and fixed to the heat insulation component 2227 via the fourth mounting component. The fourth mounting component includes, but is not limited to, screws, pins, or snap-fit ​​components.

[0166] Optionally, the heater 2222 is bonded and fixed to the heat-conducting element 2226.

[0167] Optionally, the support base 223 is connected to the heat-conducting element 2226, the heat-insulating element 2227, or the support plate 2221, and the support base 223 is used to support the carrier 40. When the carrier 40 is placed on the support base 223, one side of the carrier 40 abuts against the heater 2222, and the other side of the carrier 40 faces the optical detection component.

[0168] For example, the clamping member 2343 has a first position and a second position. When the clamping member 2343 moves to the first position, it clamps the carrier 40, causing the carrier 40 to abut against the temperature regulating mechanism 222, specifically, for example, in close contact with the heater 2222. When the clamping member 2343 moves to the second position, there is a gap between the clamping member 2343 and the carrier 40. Optionally, the gap between the clamping member 2343 and the carrier 40 may include, but is not limited to, 1 mm to 4 mm, specifically, for example, 1 mm, 2 mm, 3 mm, or 4 mm.

[0169] The material of the heat spreader 22223 is usually ceramic. During the process of inserting the carrier 40 into the slot 2231, the heat spreader 22223 is easily bumped and cracked, which seriously affects the uniformity of the surface temperature of the heat spreader 22223.

[0170] For example, the top of the slot 2231, near the temperature regulating mechanism 222, has a boss 2232 protruding towards the fluorescence detection assembly 23. The distance between the bottom surface of the boss 2232 and the bottom wall of the slot 2231 is greater than or equal to the length of the carrier 40. When the carrier 40 is inserted into the slot 2231 from top to bottom, the boss 2232 abuts against the carrier 40, creating a gap between the carrier 40 and the temperature regulating mechanism 222. Specifically, for example, a gap is formed with the surface of the heat spreader 22223. (See example for the gap.) Figure 10 As shown in S. Optionally, the spacing S is not limited to 1mm to 5mm, specifically 1mm, 2mm, 3mm, 4mm or 5mm, which can prevent the temperature regulating mechanism 222 from contacting the carrier 40, thereby avoiding damage to the temperature regulating mechanism 222 by collision with the carrier 40.

[0171] Furthermore, since the distance between the bottom surface of the boss 2232 and the bottom wall of the slot 2231 is greater than or equal to the length of the carrier 40, that is, when the carrier 40 is inserted into place, the carrier 40 can avoid the boss 2232. Under the clamping force of the clamping mechanism 234, the carrier 40 can abut against and press against the temperature regulating mechanism 222. In other words, the boss 2232 will not affect the heating of the carrier 40 by the temperature regulating mechanism 222, and the distance between the carrier 40 and the fluorescence detection component 23 remains constant.

[0172] It should be noted that the direction in which the carrier 40 is inserted into the slot 2231 and the direction in which it is removed from the slot 2231 are set vertically. See the following for details: Figure 9 The double arrow Z is shown in the diagram.

[0173] To facilitate the insertion of the carrier 40 into the slot 2231, for example, the top of the boss 2232 is provided with a first guide surface 22321, and the distance between the first guide surface 22321 and the temperature regulating mechanism 222 increases along the insertion direction of the carrier 40. Optionally, the first guide surface 22321 is, for example, an arc-shaped surface or a slope, etc. The first guide surface 22321 guides the carrier 40, so that the carrier 40 can be smoothly inserted into the slot 2231.

[0174] To facilitate the upward removal of the carrier 40 from the slot 2231, for example, the bottom end of the boss 2232 is provided with a second guide surface 22322. Along the removal direction of the carrier 40, the distance between the second guide surface 22322 and the temperature regulating mechanism 222 tends to increase. Optionally, the second guide surface 22322 may be, for example, an arc-shaped surface or a slope, etc. The second guide surface 22322 guides the carrier 40, allowing the carrier 40 to be smoothly removed from the slot 2231.

[0175] Please see Figure 13 and Figure 23 In one specific embodiment, the support layer 22221 is made of FR4 material, and its thickness is, for example, 0.2 mm. Three auxiliary heating elements 22224 are used, each with a room temperature resistance of 130Ω-160Ω. Figure 23 As shown, when 0 mA and 100 mA currents are applied to the intermediate coil respectively, it can be seen that the temperature adjustment range of the intermediate coil under the action of 100 mA current is about 3.6℃. That is to say, 100 mA current can adjust the temperature unevenness of 3.6℃ to make the temperature tend to be uniform.

[0176] In the description of this application, it should be understood that if terms such as "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" appear, these terms indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0177] Furthermore, where the terms "first" and "second" appear, these terms are for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined with "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, where the term "multiple" appears, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0178] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0179] In this application, unless otherwise expressly specified and limited, the use of descriptions such as "above" or "below" the second feature indicates that the first and second features are in direct contact or indirect contact via an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. Similarly, "below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0180] It should be noted that if an element is referred to as being "fixed to" or "set on" another element, it can be directly on the other element or there may be an intervening element. If an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intervening element. If so, the terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used in this application are for illustrative purposes only and do not represent the only possible implementation.

[0181] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0182] The embodiments described above are merely illustrative of several implementation methods of this application, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent application. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the protection scope of this application. Therefore, the protection scope of this patent application should be determined by the appended claims.

Claims

1. A nucleic acid amplification detection module, characterized in that, include: A temperature control mechanism is provided to regulate the temperature of the carrier so that the nucleic acid of the reaction sample within the carrier can be amplified. A fluorescence detection mechanism is provided, which is arranged at an interval relative to the temperature control mechanism. The fluorescence detection mechanism is used to detect the reaction sample after nucleic acid amplification. A pressing mechanism is used to press the carrier against the temperature regulating mechanism or release the carrier along the arrangement direction of the fluorescence detection mechanism and the temperature regulating mechanism; A first power mechanism is connected to the clamping mechanism and provides power to move the clamping mechanism to clamp or release the carrier; the clamping mechanism can avoid the fluorescence detection mechanism along its direction of movement. A first support member, the pressing mechanism being connected to the first support member; the pressing mechanism includes: a guide member, a movable frame, and a pressing member; the guide member is connected to the first support member; the movable frame is slidably disposed on the guide member; the pressing member is connected to the movable frame, the movable frame being able to drive the pressing member to move, causing the pressing member to press against or release the carrier; the pressing member includes a pressing head and a bearing plate; the bearing plate has an opening, the opening being able to avoid blocking the detection light of the fluorescence detection mechanism, the pressing head being arranged circumferentially around the opening; and A second support member is connected between the fluorescence detection mechanism and the first support member, with the fluorescence detection mechanism located between the clamping member and the second support member. The movable frame has an opening for avoiding the second support member, with the second support member passing through the opening. The movable frame includes a movable plate and a third support member. The third support member is connected between the clamping member and the movable plate. Multiple third support members are provided, and these multiple third support members are arranged at intervals around the outer periphery of the second support member and the fluorescence detection mechanism.

2. The nucleic acid amplification and detection module according to claim 1, characterized in that, It also includes a support base, which is located on the side of the temperature regulation mechanism facing the fluorescence detection mechanism, and the support base is provided with a slot for inserting the carrier.

3. The nucleic acid amplification and detection module according to claim 2, characterized in that, The slot extends through the support base along the arrangement direction of the fluorescence detection mechanism and the temperature regulation mechanism.

4. The nucleic acid amplification and detection module according to claim 3, characterized in that, The top of the slot, near the temperature regulation mechanism, has a protrusion facing the fluorescence detection mechanism; the distance between the bottom surface of the protrusion and the bottom wall of the slot is greater than or equal to the length of the carrier.

5. The nucleic acid amplification and detection module according to claim 4, characterized in that, The top of the boss is provided with a first guide surface, and the distance between the first guide surface and the temperature adjustment mechanism increases along the insertion direction of the carrier; and / or, the bottom of the boss is provided with a second guide surface, and the distance between the second guide surface and the temperature adjustment mechanism increases along the removal direction of the carrier.

6. The nucleic acid amplification and detection module according to claim 3, characterized in that, The first power mechanism includes a motor; the clamping mechanism further includes: Nut, the nut being connected to the movable frame; and A lead screw, which passes through the nut, and is connected to the shaft of the motor.

7. The nucleic acid amplification and detection module according to claim 6, characterized in that, The clamping mechanism also includes a coupling, which is installed on the first support member. The lead screw is coaxially connected to the coupling, and the coupling is connected to the shaft of the motor.

8. The nucleic acid amplification and detection module according to claim 6, characterized in that, One end of the guide is connected to the first support, and the other end of the guide is detachably connected to the temperature regulating mechanism or the support base.

9. The nucleic acid amplification and detection module according to claim 6, characterized in that, The pressure head is made of heat-insulating material and is used to press or release the carrier.

10. The nucleic acid amplification and detection module according to claim 6, characterized in that, The support plate includes a metal plate and / or a rigid non-metallic plate.

11. The nucleic acid amplification and detection module according to claim 6, characterized in that, The nut is connected to the movable plate, the movable plate is slidably disposed on the guide member, the movable plate is spaced apart from the first support member, and the fluorescence detection mechanism is located on the side of the movable plate opposite to the first support member.

12. The nucleic acid amplification and detection module according to claim 3, characterized in that, The nucleic acid amplification detection module also includes a housing, which has a window and a cover plate that is slidably disposed at the window, with the window positioned opposite to the slot.

13. The nucleic acid amplification and detection module according to claim 12, characterized in that, The nucleic acid amplification detection module also includes a second power mechanism, which is connected to the cover plate. The second power mechanism is used to drive the cover plate to slide, so as to open or close the window.

14. The nucleic acid amplification and detection module according to claim 13, characterized in that, The nucleic acid amplification detection module also includes a mounting frame, which is connected to the housing, and the first power mechanism and the second power mechanism are mounted on the mounting frame.

15. The nucleic acid amplification and detection module according to claim 14, characterized in that, The mounting bracket is detachably connected to the first support member.

16. The nucleic acid amplification and detection module according to claim 14, characterized in that, The housing is disposed on top of the temperature regulation mechanism and the fluorescence detection mechanism; the nucleic acid amplification detection module also includes a light shield, which is connected to the housing or the mounting bracket and is disposed outside the fluorescence detection mechanism.

17. The nucleic acid amplification detection module according to any one of claims 1 to 16, characterized in that, The temperature regulating mechanism includes a heater for contacting the carrier to raise the temperature of the carrier. The heater includes a support layer, a main heating element, and a heat spreader plate. The main heating element is connected to the support layer, and the heat spreader plate is connected to the support layer.

18. The nucleic acid amplification and detection module according to claim 17, characterized in that, The heater also includes an auxiliary heating element, which is connected to the temperature distribution plate.

19. The nucleic acid amplification and detection module according to claim 18, characterized in that, The auxiliary heating element is provided in multiple parts, and the multiple auxiliary heating elements are respectively disposed in multiple different parts of the heat exchange plate.

20. The nucleic acid amplification and detection module according to claim 18, characterized in that, The main heating element includes a main heating coil; and / or, the auxiliary heating element includes an auxiliary heating coil.

21. The nucleic acid amplification and detection module according to claim 18, characterized in that, The main heating element is located between the support layer and the heat exchange plate; the auxiliary heating element is located on the side of the heat exchange plate opposite to the support layer.

22. The nucleic acid amplification and detection module according to claim 18, characterized in that, The heater further includes a first lead and a second lead. The first lead is electrically connected to the auxiliary heating element and is used to connect to a voltage testing circuit. The second lead is electrically connected to the auxiliary heating element and is used to connect to a current supply circuit.

23. The nucleic acid amplification and detection module according to claim 18, characterized in that, The materials of the support layer include composite boards of epoxy resin and glass fiber, ceramics, polyethylene terephthalate and / or glass.

24. The nucleic acid amplification and detection module according to claim 17, characterized in that, The temperature regulating mechanism also includes a radiator connected to the heater, which is used to reduce the temperature of the heater.

25. The nucleic acid amplification and detection module according to claim 24, characterized in that, The temperature regulating mechanism also includes a heat-conducting element, which is connected between the heater and the radiator.

26. The nucleic acid amplification and detection module according to claim 25, characterized in that, The temperature regulating mechanism further includes: a first circuit board and a support plate, wherein the first circuit board is electrically connected to the heater and the support plate is connected to the heater.

27. The nucleic acid amplification and detection module according to claim 26, characterized in that, The first circuit board is provided with a first clearance portion, the support plate is provided with a second clearance portion, the support plate is provided with a second clearance portion that is connected to the first clearance portion, and the heat-conducting component passes through the second clearance portion and the first clearance portion in sequence and is connected to the heat sink.

28. The nucleic acid amplification and detection module according to claim 27, characterized in that, The heat-conducting component has a protrusion that passes through the second clearance portion and the first clearance portion in sequence and is connected to the radiator; the support plate has a heat insulation component on the side near the heater; the heat insulation component is disposed between the heat-conducting component and the support plate.

29. The nucleic acid amplification detection module according to any one of claims 13 to 16, characterized in that, The nucleic acid amplification and detection module also includes a first controller, and the temperature regulation mechanism, the fluorescence detection mechanism, the first power mechanism and the second power mechanism are all electrically connected to the first controller.

30. The nucleic acid amplification and detection module according to claim 29, characterized in that, The nucleic acid amplification detection module further includes a first circuit board, a second circuit board, and a third circuit board. The first circuit board is provided with a first electrical connector, and the second circuit board is provided with a second electrical connector. The first controller is provided with a third electrical connector and a fourth electrical connector. The first electrical connector and the third electrical connector are plugged into each other, and the second electrical connector and the fourth electrical connector are plugged into each other. The third circuit board is provided with a fifth electrical connection connector, the first controller is provided with a sixth electrical connection connector, the first power mechanism and the second power mechanism are both electrically connected to the fifth electrical connection connector, and the fifth electrical connection connector and the sixth electrical connection connector are plugged into each other.

31. The nucleic acid amplification and detection module according to claim 1, characterized in that, The fluorescence detection mechanism is configured as multiple; the multiple fluorescence detection mechanisms are arranged in sequence.

32. A nucleic acid amplification and detection device, characterized in that, The device includes a nucleic acid amplification detection module as described in any one of claims 1 to 31, and further includes a housing, wherein the housing is provided with a mounting portion, and the nucleic acid amplification detection module is mounted on the mounting portion.

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