一种基于SSR标记与光温调控技术的小麦重组自交系群体高效构建方法

The method of constructing wheat recombinant inbred lines by optimizing the screening of SSR markers and light and temperature regulation technology has solved the problems of parental screening errors and difficulties in hybridization identification, shortened the population construction cycle and improved genetic stability, and is applicable to wheat breeding and genetic improvement.

CN121006413BActive Publication Date: 2026-07-17YANTAI ACAD OF AGRI SCI SHANDONG PROVINCE (YANTAI BRANCH OF SHANDONG ACAD OF AGRI SCI)

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
YANTAI ACAD OF AGRI SCI SHANDONG PROVINCE (YANTAI BRANCH OF SHANDONG ACAD OF AGRI SCI)
Filing Date
2025-08-20
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

In the current process of constructing wheat recombinant inbred line populations, parental selection is easily affected by the environment, the authenticity of hybridization is difficult to identify, the population purification cycle is long, and the efficiency is low.

Method used

SSR markers were used to screen parental combinations, and photothermal regulation technology was combined to optimize the hybridization verification and generation progression process. 21 pairs of SSR primers were used to detect genomic differential sites to achieve rapid and accurate hybridization and population purification.

Benefits of technology

It shortens the population construction cycle, improves genetic stability, and enables the efficient construction of wheat recombinant inbred line populations. It is suitable for genetic map construction, quantitative trait locus localization, gene cloning, and molecular design breeding.

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Abstract

本发明公开了一种基于SSR标记与光温调控技术的小麦重组自交系群体高效构建方法,属于作物遗传育种技术领域。所述构建方法包括:采用序列为SEQ ID NO.1‑42所示的21对SSR引物PM01‑PM21对亲本进行PCR检测,筛选基因组差异位点≥4个的亲本组合进行杂交获得F1代;在F1代群体中,利用亲本间差异位点对应的引物检测杂合位点,筛选符合双亲遗传互补特征的植株自交获得F2代;采用连续6代单粒传法结合光温调控技术,完成F2至F8代的世代推进,获得小麦重组自交系群体。春小麦每代繁育约需2个月,冬小麦每代约需3个月,结合SSR标记筛选F1代,可提升RIL群体构建的效率和准确率。
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