Gastrointestinal ultrasonic developing aid and preparation method thereof

Abstract

The invention provides a gastrointestinal ultrasonic developing aid and a preparation method thereof. The gastrointestinal ultrasonic developing aid comprises a fructus amomi extract, rice, soybeans, coix seeds and Chinese yams. Wherein the fructus amomi extract is prepared by adopting a specific method, the developing aid provided by the invention can be used for rapidly filling a gastral cavity and an enteric cavity, so that an acoustic impedance difference is formed on a medium surface, and ultrasonic artifacts and interferences such as reverberation effect generated in ultrasonic examination and artifacts and pattern distortion caused by high and low attenuation areas of a medium are eliminated; therefore, the sound image definition of the examined part and peripheral organs is improved, and the sound image resolution of the focus is enhanced, so that the system is suitable for ultrasonic examination and diagnosis of gastrointestinal tracts; according to the auxiliary developing agent provided by the invention, the fructus amomi extract is prepared by adopting a specific method, and the fructus amomi extract is matched with the rice, the soybeans, the coix seeds and the Chinese yams for use, so that the gastric mucosal injury can be relieved, the influence of gastric mucus on mucosal layer development is effectively relieved, and the sound image definition of a detected part is remarkably improved.

Description

Technical Field

[0001] This invention belongs to the field of ultrasound contrast imaging technology, specifically relating to a gastrointestinal ultrasound contrast agent and its preparation method. Background Technology

[0002] Nowadays, the immense pressure of work and life, coupled with unhealthy dietary habits, has led to many people suffering from stomach ailments. Currently, the clinical diagnosis of many gastrointestinal diseases relies primarily on X-ray barium meal examination and gastroscopy, such as for gastric ulcers and duodenal ulcers. However, both of these examinations involve some trauma and pain, have limitations, and are not easily popularized, especially among children, pregnant women, and the elderly or those with weakened immune systems. This can also be difficult for asymptomatic individuals to accept, leading to misdiagnosis and mistreatment, and sometimes even delaying treatment until gastrointestinal perforation or massive bleeding occurs. Gastric ultrasound, with its advantages of being simple, effective, painless, and quick, is the preferred initial screening method for stomach diseases.

[0003] Ultrasound imaging is one of the most important diagnostic methods in medicine. Ultrasound waves have three main effects on matter: First, mechanical effect: due to their enormous energy, ultrasound waves often cause particles to vibrate at high frequencies, resulting in fragmentation, aggregation, and agitation effects. Second, thermal effect: when ultrasound waves act on matter, their energy is absorbed by the matter, causing an increase in temperature. Third, cavitation effect: when ultrasound waves act on a liquid, areas with weak internal stress (such as microscopic bubbles) become less dense due to the significant change in sound pressure. Under the pulling force of surrounding liquid molecules, a small cavity is formed, which is called cavitation. These effects form the physical basis of ultrasound imaging and may also trigger potential effects such as particle aggregation, tissue displacement, local overheating, cell damage, or cavitation artifacts, affecting the diagnostic accuracy of ultrasound.

[0004] In gastrointestinal ultrasound examinations, gastrointestinal gas has a strong reflective and scattering effect on ultrasound waves. When ultrasound encounters gas, most of it is reflected or scattered, resulting in the inability of the structures behind the gas to be displayed through ultrasound echoes, leading to poor image quality and low lesion detection rate.

[0005] Gastrointestinal filling ultrasound is a popular screening method for gastrointestinal diseases in recent years. It involves filling the gastrointestinal lumen with an ultrasound contrast agent via oral or intravenous administration, eliminating interference from gas and contents within the stomach and improving the internal environment for ultrasound imaging. This allows for a clearer view of the gastrointestinal wall structure and any lesions. The procedure is painless, radiation-free, safe, and convenient. Highly effective contrast agents directly enhance the diagnostic capabilities for gastrointestinal lesions.

[0006] CN1073859A discloses a fast-dissolving gastrointestinal ultrasound enhancement agent and its preparation method. It is a food and medicinal food mixed formula composed of lipid-containing plant protein, rice and wheat starch, yam, Job's tears, dried tangerine peel, and flavoring agents. The ingredients are puffed, cooked, and dried at low temperature, then pulverized and mixed to form fast-dissolving granules. This enhancement agent has been proven in over 500 clinical trials to meet the clinical requirements for gastrointestinal ultrasound diagnosis, effectively eliminate gastrointestinal artifacts, significantly improve the clarity of the examined area, and provide rapid imaging. It is also convenient to prepare, has a good taste, and causes no trauma or side effects to the human body.

[0007] CN111053924A discloses a gastrointestinal ultrasound contrast agent, comprising chicken gizzard lining extract and burdock extract. Using these extracts can eliminate interference from gas and mucus in the gastrointestinal tract, allowing for sufficient retention time in the stomach and duodenum. This enables thorough observation of the stomach and duodenal wall layers, structure, size, peristalsis, adjacent organs, and lymph nodes. Furthermore, the chicken gizzard lining extract and burdock extract also possess certain therapeutic effects for gastrointestinal diseases.

[0008] CN105106978A discloses a traditional Chinese medicine preparation for ultrasound-assisted imaging and its preparation method. The preparation comprises the following raw materials: coriander seed, rose leaf, cyperus rhizome, Evodia fruit, Solanum nigrum seed, bitter herb, Vitex leaf, Chaenomeles speciosa root, Acorus tatarinowii, Albizia bark, otolith, red-backed vine, Bauhinia fruit, winter melon seed, cattail seed husk, primrose root, *Physalis alkekengi*, and jasmine flower. This preparation is low-cost, convenient to use, safe, and has no toxic side effects. It can improve the proportion of high-quality imaging, enhance examination accuracy, and enable more gastrointestinal diseases to be examined via ultrasound, providing strong evidence for clinical diagnosis. Furthermore, the preparation process is simple and practical.

[0009] Therefore, developing a gastrointestinal ultrasound imaging aid that can effectively eliminate gastrointestinal artifacts, reduce the influence of mucus on mucosal layer imaging, significantly improve the sonographic clarity of the examined area, and provide rapid imaging is a key research focus in this field. Summary of the Invention

[0010] To address the shortcomings of existing technologies, the present invention aims to provide a gastrointestinal ultrasound imaging aid and its preparation method.

[0011] To achieve this objective, the present invention adopts the following technical solution:

[0012] In a first aspect, the present invention provides a gastrointestinal ultrasound imaging aid, the gastrointestinal ultrasound imaging aid comprising Amomum villosum extract, rice, soybean, Coix seed, and yam.

[0013] Preferably, by weight, the gastrointestinal ultrasound enhancement agent comprises 3-8 parts of Amomum villosum extract (e.g., 4, 5, 6, 7 parts, etc.), 10-25 parts of rice (e.g., 12, 14, 16, 18, 20, 22, 24 parts, etc.), 5-15 parts of soybeans (e.g., 6, 8, 10, 12, 14 parts, etc.), 10-20 parts of Coix seed (e.g., 12, 14, 16, 18 parts, etc.), and 15-25 parts of yam (e.g., 16, 18, 20, 22, 24 parts, etc.).

[0014] Preferably, the gastrointestinal ultrasound enhancement agent further includes 0-10 parts of dried tangerine peel (e.g., 2 parts, 4 parts, 6 parts, 8 parts, etc.) and 0-10 parts of corn (e.g., 2 parts, 4 parts, 6 parts, 8 parts, etc.).

[0015] Preferably, the amomum extract is prepared by the following method, the method comprising:

[0016] (1) The dried Amomum villosum powder was extracted with ethanol solution I 2-4 times, the extracts were combined and concentrated under reduced pressure to obtain Amomum villosum extract;

[0017] (2) The amomum extract was dissolved in ethanol solution II and added to a polyamide column. The column was then eluted with ethanol solution III and ethanol solution IV in sequence. The eluent of ethanol solution IV was collected, concentrated, and freeze-dried to obtain the amomum extract.

[0018] Preferably, the particle size of the cardamom powder is 50-100 mesh, for example, it can be 60 mesh, 70 mesh, 80 mesh, 90 mesh, etc.

[0019] Preferably, the mass ratio of the amomum extract to ethanol solution II is 1:(3-5), for example, it can be 1:3.5, 1:4, 1:4.5, etc.

[0020] Preferably, the particle size of the polyamide dry powder in the polyamide column is 50-100 mesh, for example, it can be 60 mesh, 70 mesh, 80 mesh, 90 mesh, etc.

[0021] Preferably, the mass ratio of polyamide powder to amomum extract in the polyamide column is (5-10):1, for example, it can be 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, etc.

[0022] Preferably, the ethanol solution I contains 85-95% ethanol by mass, for example, 86%, 88%, 90%, 92%, 94%, etc.

[0023] Preferably, the ethanol solution II contains 10-30% ethanol by mass, for example, 15%, 20%, 25%, etc.

[0024] Preferably, the ethanol content in the ethanol solution III is 20-35% by mass, for example, 22%, 24%, 26%, 28%, 30%, 32%, 34%, etc.

[0025] Preferably, the ethanol content in the ethanol solution IV is 40-80% by mass, for example, it can be 45%, 50%, 55%, 60%, 65%, 70%, 75%, etc.

[0026] Preferably, the amount of ethanol solution III is 2-3 times the column volume, for example, 2.2 times, 2.4 times, 2.6 times, 2.8 times, etc.

[0027] Preferably, the amount of ethanol solution IV used is 3-5 times the column volume, for example, 3.5 times, 4 times, 4.5 times, etc.

[0028] Preferably, the preparation method of the rice, soybeans, coix seed, yam and optionally dried tangerine peel and corn is as follows: the raw materials are crushed, dried and sieved to obtain the final product.

[0029] Preferably, the drying temperature is 80-120℃, such as 85℃, 90℃, 95℃, 100℃, 105℃, 110℃, 115℃, etc., and the time is 5-6h, such as 5.2h, 5.4h, 5.6h, 5.8h, etc.

[0030] Preferably, the sieve used for sieving is 80-150 mesh, for example, it can be 85 mesh, 90 mesh, 95 mesh, 100 mesh, 110 mesh, 120 mesh, 130 mesh, 140 mesh, etc.

[0031] Secondly, the present invention provides a method for preparing a gastrointestinal ultrasound imaging aid as described in the first aspect, the method comprising: dissolving Amomum villosum extract, rice, soybean, Coix seed, and yam in water, sterilizing, and thus obtaining the aid.

[0032] The numerical range described in this invention includes not only the point values ​​listed above, but also any point values ​​within the numerical ranges not listed above. Due to space limitations and for the sake of brevity, this invention will not exhaustively list all the specific point values ​​included in the range.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] (1) The contrast agent provided by the present invention can quickly fill the gastric cavity and intestinal cavity, so that the medium surface forms an acoustic impedance difference, which eliminates the reverberation effect and artifacts and image distortion caused by the high and low attenuation zones of the medium during ultrasound examination, thereby improving the clarity of the ultrasound image of the examined site and surrounding organs, and enhancing the resolution of the lesion ultrasound image, thus making it suitable for ultrasound examination and diagnosis of the gastrointestinal tract.

[0035] (2) The imaging aid provided by the present invention uses a specific method to prepare Amomum villosum extract. When it is used in combination with rice, soybean, coix seed and yam, it can alleviate gastric mucosal damage, effectively reduce the influence of gastric mucus on mucosal layer imaging, and significantly improve the sonographic clarity of the examined area. Attached Figure Description

[0036] Figure 1 The image shows the appearance of the liquid formulation obtained by dissolving the gastrointestinal ultrasound enhancement agent prepared in Example 1 in water.

[0037] Figure 2 Ultrasound scans of the stomach at different angles using the gastrointestinal ultrasound enhancement agent prepared in Example 1.

[0038] Figure 3 An ultrasound scan of the stomach using the gastrointestinal ultrasound enhancement agent prepared for Comparative Example 1.

[0039] Figure 4 Ultrasound scan of the stomach using the gastrointestinal ultrasound enhancement agent prepared for Comparative Example 2. Detailed Implementation

[0040] The technical solution of the present invention will be further illustrated below through specific embodiments. Those skilled in the art should understand that the embodiments described are merely illustrative of the present invention and should not be construed as limiting the invention in any way.

[0041] The terms “comprising,” “including,” “having,” “containing,” or any other variations thereof, as used herein, are intended to cover non-exclusive inclusion. For example, a composition, step, method, article, or apparatus that includes the listed elements is not limited to those elements and may also include other elements not expressly listed or elements inherent to such composition, step, method, article, or apparatus.

[0042] "Optional" or "any one" means that the matter or event described thereafter may or may not occur, and the description includes both the possibility that the event may occur and the possibility that the event may not occur.

[0043] The indefinite articles “a” and “an” preceding an element or component of this invention do not impose any limitation on the quantity (i.e., number of times) of the element or component. Therefore, “an” or “a” should be interpreted as including one or at least one, and the singular form of an element or component also includes the plural form, unless the quantity clearly refers only to the singular form.

[0044] The terms "one embodiment," "some embodiments," "exemplary," "specific example," or "some examples," etc., used in this invention refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the invention. In this document, the illustrative expressions of the above terms are not necessarily directed at the same embodiment or example.

[0045] In this invention, unless otherwise explicitly stated, percentages and contents are all by mass. Unless otherwise specified, the experimental methods used are conventional methods, and the materials and reagents used are commercially available.

[0046] The rice, soybeans, Job's tears, yam, dried tangerine peel, and corn mentioned in the following examples refer to the raw material powder obtained by drying the raw materials at 100℃ for 6 hours, pulverizing them, and passing them through a 100-mesh sieve.

[0047] Preparation Example 1

[0048] This preparation example provides a cardamom extract, prepared by the following method:

[0049] (1) Extract 100-mesh dried Amomum villosum powder three times with three times the amount of 90% ethanol solution, combine the extracts, and concentrate under reduced pressure to obtain Amomum villosum extract;

[0050] (2) Dissolve the Amomum villosum extract in 4 times the amount of 20% ethanol solution, add it to an 80-mesh polyamide column, and use 8 times the amount of polyamide dry powder. Elute sequentially with 2 column volumes of 30% ethanol solution and 4 column volumes of 60% ethanol solution. Collect the eluent of 60% ethanol solution, concentrate, freeze dry, and pulverize into fine powder to obtain the Amomum villosum extract.

[0051] Preparation Example 2

[0052] This preparation example provides a cardamom extract, prepared by the following method:

[0053] (1) Extract 100-mesh dried Amomum villosum powder three times with twice the amount of 95% ethanol solution, combine the extracts, and concentrate under reduced pressure to obtain Amomum villosum extract;

[0054] (2) Dissolve the Amomum villosum extract in 5 times the amount of 10% ethanol solution, add it to a 100-mesh polyamide column, and use 5 times the amount of polyamide dry powder. Elute sequentially with 3 column volumes of 20% ethanol solution and 3 column volumes of 80% ethanol solution. Collect the eluent of 80% ethanol solution, concentrate, freeze dry, and pulverize into fine powder to obtain the Amomum villosum extract.

[0055] Preparation Example 3

[0056] This preparation example provides a cardamom extract, prepared by the following method:

[0057] (1) Extract 100-mesh dried Amomum villosum powder three times with 4 times the amount of 85% ethanol solution, combine the extracts, and concentrate under reduced pressure to obtain Amomum villosum extract.

[0058] (2) Dissolve the Amomum villosum extract in 3 times the amount of 30% ethanol solution, add it to a 50-mesh polyamide column, and use 10 times the amount of polyamide dry powder. Elute sequentially with 2 column volumes of 35% ethanol solution and 5 column volumes of 40% ethanol solution. Collect the eluent of 40% ethanol solution, concentrate, freeze dry, and pulverize into fine powder to obtain the Amomum villosum extract.

[0059] Comparative Preparation Example 1

[0060] This comparative preparation example provides a cardamom extract, prepared by the following method:

[0061] (1) Extract 100-mesh dried Amomum villosum powder three times with 4 times the amount of 85% ethanol solution, combine the extracts, and concentrate under reduced pressure to obtain Amomum villosum extract.

[0062] (2) The amomum extract is freeze-dried and pulverized into fine powder to obtain the amomum extract.

[0063] Example 1

[0064] This embodiment provides a gastrointestinal ultrasound enhancement agent, which, by weight, comprises: 20 parts rice, 10 parts soybeans, 15 parts coix seed, 20 parts yam, 5 parts dried tangerine peel, 3 parts corn, and 5 parts of Amomum villosum extract from Preparation Example 1.

[0065] The above raw materials are mixed evenly according to the formula to obtain the gastrointestinal ultrasound enhancement agent.

[0066] Example 2

[0067] This embodiment provides a gastrointestinal ultrasound enhancement agent, which, by weight, comprises: 10 parts rice, 15 parts soybeans, 10 parts coix seed, 25 parts yam, 5 parts dried tangerine peel, 6 parts corn, and 8 parts of the amomum villosum extract from Preparation Example 2.

[0068] The above raw materials are mixed evenly according to the formula to obtain the gastrointestinal ultrasound enhancement agent.

[0069] Example 3

[0070] This embodiment provides a gastrointestinal ultrasound enhancement agent, which, by weight, comprises: 25 parts rice, 5 parts soybeans, 20 parts coix seed, 15 parts yam, 2 parts dried tangerine peel, 3 parts corn, and 3 parts of Amomum villosum extract from Preparation Example 3.

[0071] The above raw materials are mixed evenly according to the formula to obtain the gastrointestinal ultrasound enhancement agent.

[0072] Example 4

[0073] This embodiment provides a gastrointestinal ultrasound enhancement agent, which, by weight, comprises: 20 parts rice, 10 parts soybeans, 15 parts coix seed, 20 parts yam, and 5 parts of the amomum villosum extract from Preparation Example 1;

[0074] The above raw materials are mixed evenly according to the formula to obtain the gastrointestinal ultrasound enhancement agent.

[0075] Comparative Example 1

[0076] This comparative example provides a gastrointestinal ultrasound enhancement agent, which differs from Example 1 only in that the Amomum villosum extract of Preparation Example 1 is replaced with an equal amount of Amomum villosum extract of Comparative Preparation Example 1; other raw materials, dosages and preparation methods are the same as in Example 1.

[0077] Comparative Example 2

[0078] This comparative example provides a gastrointestinal ultrasound enhancement agent, which differs from Example 1 only in that the Amomum villosum extract of Preparation Example 1 is not added; other raw materials, dosages and preparation methods are the same as in Example 1.

[0079] Comparative Example 3

[0080] This comparative example provides a gastrointestinal ultrasound enhancement agent, which differs from Example 1 only in that rice is not added; other raw materials, dosages and preparation methods are the same as in Example 1.

[0081] Comparative Example 4

[0082] This comparative example provides a gastrointestinal ultrasound enhancement agent, which differs from Example 1 only in that soybeans are not added; other raw materials, dosages and preparation methods are the same as in Example 1.

[0083] Comparative Example 5

[0084] This comparative example provides a gastrointestinal ultrasound enhancement agent, which differs from Example 1 only in that it does not contain coix seed; other raw materials, dosages and preparation methods are the same as in Example 1.

[0085] Comparative Example 6

[0086] This comparative example provides a gastrointestinal ultrasound enhancement agent, which differs from Example 1 only in that it does not contain yam; other raw materials, dosages and preparation methods are the same as in Example 1.

[0087] Test Example 1

[0088] For example, a liquid formulation is obtained by dissolving the gastrointestinal ultrasound enhancement agent prepared in Example 1 in water. The liquid formulation has a water content of 90 wt% and its appearance is as follows. Figure 1 As shown in the figure, the ultrasound enhancement agent provided by the present invention, when mixed with water, forms a thick liquid with good adhesion to the wall.

[0089] Test Example 2

[0090] Acute toxicity test

[0091] Test liquid formulation: The liquid formulation (water content of 90 wt%) obtained by dissolving the gastrointestinal ultrasound enhancement agents prepared in each example and comparative example in water, is prepared for use.

[0092] Experimental animals: ordinary grade mice, weighing 20g±5g, half male and half female, and none of the female mice were pregnant.

[0093] When mice were administered each of the tested liquid preparations by gavage, when the gavage dose reached 30 mL / mouse, the mice showed a slight decrease in activity after administration, which returned to normal in about 1 hour. After 7 days of continuous observation after administration, no animals died. There were no significant differences in blood counts, liver function, total protein, albumin, globulin and kidney function before and after administration. The mice's overall condition, diet, water intake, urination and weight gain were all normal.

[0094] The experimental results show that the gastrointestinal ultrasound imaging agent provided by this invention, administered to mice by gavage, exhibits extremely low or no acute toxicity and is safe for clinical use.

[0095] Test Example 3

[0096] Anti-gastric mucosal injury experiment

[0097] Test liquid formulation: The liquid formulation (water content of 90 wt%) obtained by dissolving the gastrointestinal ultrasound enhancement agents prepared in each example and comparative example in water, is prepared for use.

[0098] Experimental Methods: SPF-grade SD rats, half male and half female, weighing 150-200g, were randomly divided into a control group, Example 1-4 groups, and Comparative Example 1-6 groups, with 6 rats in each group. Animals were administered the drug by gavage once daily for 3 consecutive days, while the control group received an equal volume of distilled water. Before the experiment, rats were fasted for 24 hours but had free access to water. One hour after the last administration, each rat was administered 1 mL of a hydrochloric acid-ethanol mixture (150 mmol / L hydrochloric acid - 60% ethanol) by gavage. One hour later, the animals were sacrificed, and the entire stomach was removed. After fixation with 1% formaldehyde for 15 minutes, the stomach wall was cut along the greater curvature, and the lesion length was measured. The total ulcer length in millimeters was used as the ulcer index, and the inhibition rate was calculated.

[0099] Inhibition rate (%) = (Ulcer index of control group - Ulcer index of treatment group) / Ulcer index of control group × 100%. The test results are shown in Table 1.

[0100] Table 1

[0101] sample Inhibition rate (%) Example 1 70％ Example 2 68％ Example 3 65％ Example 4 62％ Comparative Example 1 50％ Comparative Example 2 34％ Comparative Example 3 60％ Comparative Example 4 58％ Comparative Example 5 56％ Comparative Example 6 50％ control group 0

[0102] According to the data in the table, the Amomum villosum extract prepared by the method provided by this invention plays an important role in alleviating gastric mucosal damage in ultrasound-assisted imaging agents. When prepared by ordinary alcohol extraction or water extraction, its efficacy in alleviating gastric mucosal damage will decrease. When Amomum villosum extract is missing from the ultrasound-assisted imaging agent, its efficacy in alleviating gastric mucosal damage will be greatly reduced. When rice, soybean, coix seed, yam and other ingredients are missing, its efficacy in alleviating gastric mucosal damage will be reduced.

[0103] Test Example 4

[0104] Animal ultrasound testing

[0105] Test liquid formulation: The liquid formulation (water content of 90 wt%) obtained by dissolving the gastrointestinal ultrasound enhancement agents prepared in each example and comparative example in water, is prepared for use.

[0106] Experimental methods: SPF-grade SD rats, half male and half female, weighing 200-250g, were randomly divided into Example 1-4 groups and Comparative Example 1-6 groups. After fasting for 8 hours, they were fed 10mL / kg of the test liquid preparation in four divided doses over 2 hours.

[0107] Scanning method: The scan was performed using the SonoScan painless in vitro gastrointestinal imaging scanner from the United States.

[0108] Scan results:

[0109] When using the gastrointestinal ultrasound contrast agents prepared in Examples 1-4 for ultrasound detection, they can remain and fill the gastrointestinal tract for a longer period of time, producing a uniformly distributed hypoechoic interface area on the ultrasound image. This can effectively display the layers and structure of the gastrointestinal wall, enhance the display of retroperitoneal organs and the delineation of the edges of surrounding structures, and provide insight into the peristalsis and emptying function of the gastrointestinal tract. At the same time, it can eliminate the interference of gas and mucus in the gastrointestinal lumen, effectively reduce and eliminate artifacts and interference in the examination area, and facilitate the determination of the location, shape, size, extent and internal structure of lesions.

[0110] Exemplary, a gastric ultrasound scan image obtained using the gastrointestinal ultrasound contrast agent prepared in Example 1 is shown below. Figure 2 As shown, the stomach is well-filled, and the stomach wall and mucosa are clearly visible.

[0111] For example, a gastric ultrasound scan image obtained using the gastrointestinal ultrasound contrast agent prepared in Comparative Example 1 is shown below. Figure 3 As shown, the addition of traditionally prepared Amomum villosum extract as a contrast agent can help reduce ultrasound artifacts and interference caused by reverberation, artifacts caused by high and low attenuation zones of the medium, and image distortion. However, the clarity of the ultrasound images of the examined area and surrounding organs is poor, the resolution of the lesion is low, and the gastric mucus has a significant impact on the mucosal layer imaging, resulting in poor clarity of the ultrasound images of the examined area.

[0112] For example, a gastric ultrasound scan image obtained using the gastrointestinal ultrasound enhancement agent prepared in Comparative Example 2 is shown below. Figure 4 As shown, without the addition of Amomum villosum extract as a highlighting agent, ultrasound artifacts and interferences such as reverberation effect, artifacts caused by high and low attenuation zones of the medium, and image distortion are obvious during ultrasound examination. The clarity of the ultrasound images of the examined area and surrounding organs is low, the resolution of the lesion is poor, and the gastric mucus has a significant impact on the imaging of the mucosal layer, resulting in very poor clarity of the ultrasound images of the examined area.

[0113] The applicant declares that the present invention is illustrated by the above embodiments, but the present invention is not limited to the above process steps, that is, it does not mean that the present invention must rely on the above process steps to be implemented. Those skilled in the art should understand that any improvements to the present invention, equivalent substitutions of the raw materials used in the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0114] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details of the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0115] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. A gastrointestinal ultrasound enhancement agent, characterized in that, The gastrointestinal ultrasound enhancement agent includes Amomum villosum extract, rice, soybean, Job's tears, and yam.

2. The gastrointestinal ultrasound imaging aid according to claim 1, characterized in that, By weight, the gastrointestinal ultrasound enhancement agent comprises 3-8 parts of Amomum villosum extract, 10-25 parts of rice, 5-15 parts of soybean, 10-20 parts of Coix lacryma-jobi, and 15-25 parts of Dioscorea opposita. Preferably, the gastrointestinal ultrasound enhancement agent further includes 0-10 parts of dried tangerine peel and 0-10 parts of corn.

3. The gastrointestinal ultrasound imaging aid according to claim 1 or 2, characterized in that, The Amomum villosum extract is prepared by the following method, the method comprising: (1) The dried Amomum villosum powder was extracted with ethanol solution I 2-4 times, the extracts were combined and concentrated under reduced pressure to obtain Amomum villosum extract; (2) The amomum extract was dissolved in ethanol solution II and added to a polyamide column. The column was then eluted with ethanol solution III and ethanol solution IV in sequence. The eluent of ethanol solution IV was collected, concentrated, and freeze-dried to obtain the amomum extract.

4. The gastrointestinal ultrasound imaging aid according to claim 3, characterized in that, The particle size of the cardamom powder is 50-100 mesh; Preferably, the mass ratio of the amomum extract to ethanol solution II is 1:(3-5).

5. The gastrointestinal ultrasound imaging aid according to claim 3 or 4, characterized in that, The particle size of the polyamide dry powder in the polyamide column is 50-100 mesh; Preferably, the mass ratio of polyamide dry powder to cardamom extract in the polyamide column is (5-10):

1.

6. The gastrointestinal ultrasound imaging aid according to any one of claims 3-5, characterized in that, The ethanol solution I contains 85-95% ethanol by mass. Preferably, the ethanol solution II contains 10-30% ethanol by mass.

7. The gastrointestinal ultrasound imaging aid according to any one of claims 3-6, characterized in that, The ethanol solution III contains 20-35% ethanol by mass. Preferably, the ethanol solution IV contains 40-80% ethanol by mass.

8. The gastrointestinal ultrasound imaging aid according to any one of claims 3-7, characterized in that, The amount of ethanol solution III used is 2-3 times the column volume; Preferably, the amount of ethanol solution IV used is 3-5 times the column volume.

9. The gastrointestinal ultrasound imaging aid according to any one of claims 1-8, characterized in that, The preparation method of the rice, soybeans, coix seed, yam, and optionally dried tangerine peel and corn is as follows: the raw materials are crushed, dried, and sieved to obtain the final product; Preferably, the drying temperature is 80-120℃ and the time is 5-6 hours; Preferably, the sieve used for sieving has a mesh size of 80-150.

10. A method for preparing a gastrointestinal ultrasound enhancement agent as described in any one of claims 1-9, characterized in that, The preparation method includes: dissolving Amomum villosum extract, rice, soybeans, Job's tears, and yam in water, sterilizing them, and then obtaining the product.

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